AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24320.t1 MTR_1g021855 97.820 367 8 0 1 367 1 367 0.0 745
MS.gene24320.t1 MTR_5g019220 76.205 332 68 3 46 367 37 367 0.0 530
MS.gene24320.t1 MTR_8g078240 73.860 329 77 4 40 366 23 344 0.0 515
MS.gene24320.t1 MTR_3g111600 86.525 282 37 1 86 367 1 281 0.0 514
MS.gene24320.t1 MTR_3g092310 62.145 317 101 4 46 360 34 333 1.86e-146 418
MS.gene24320.t1 MTR_6g008900 55.432 359 141 6 10 358 10 359 2.07e-140 404
MS.gene24320.t1 MTR_4g071620 55.432 359 141 6 10 358 10 359 2.07e-140 404
MS.gene24320.t1 MTR_7g094230 59.048 315 108 2 46 360 51 344 1.63e-138 398
MS.gene24320.t1 MTR_7g109210 54.775 356 137 6 12 358 10 350 1.79e-137 395
MS.gene24320.t1 MTR_5g036580 55.072 345 142 5 20 360 25 360 1.39e-136 395
MS.gene24320.t1 MTR_4g118030 58.675 317 112 4 46 361 42 340 3.20e-135 389
MS.gene24320.t1 MTR_1g089820 58.466 313 109 2 46 358 54 345 7.75e-133 384
MS.gene24320.t1 MTR_2g461670 32.642 193 118 6 165 356 395 576 4.71e-21 95.1
MS.gene24320.t1 MTR_4g109890 33.161 193 117 6 165 356 340 521 3.19e-20 92.4
MS.gene24320.t1 MTR_3g107930 27.843 255 171 8 111 363 337 580 4.72e-19 89.0
MS.gene24320.t1 MTR_3g107930 27.843 255 171 8 111 363 414 657 5.27e-19 89.0
MS.gene24320.t1 MTR_2g082650 30.151 199 122 8 165 356 209 397 4.36e-18 85.5
MS.gene24320.t1 MTR_2g015550 31.351 185 119 5 165 349 339 515 8.28e-18 85.1
MS.gene24320.t1 MTR_2g082650 30.151 199 122 8 165 356 338 526 8.80e-18 85.1
MS.gene24320.t1 MTR_8g092000 29.444 180 114 5 165 341 384 553 1.46e-17 84.3
MS.gene24320.t1 MTR_7g055600 28.643 199 132 6 165 363 238 426 3.48e-16 79.7
MS.gene24320.t1 MTR_7g057530 28.643 199 132 6 165 363 238 426 3.48e-16 79.7
MS.gene24320.t1 MTR_2g027740 29.444 180 114 5 165 341 471 640 4.55e-16 80.1
MS.gene24320.t1 MTR_7g075840 27.136 199 135 5 165 363 468 656 8.59e-16 79.3
MS.gene24320.t1 MTR_7g012370 28.571 182 116 6 165 339 334 508 9.46e-16 79.0
MS.gene24320.t1 MTR_4g133570 29.648 199 123 8 165 356 261 449 1.52e-15 78.2
MS.gene24320.t1 MTR_3g452360 21.835 316 188 10 86 356 65 366 1.03e-14 75.1
MS.gene24320.t1 MTR_6g016380 28.646 192 127 6 165 356 359 540 1.60e-14 75.1
MS.gene24320.t1 MTR_3g452360 21.835 316 188 10 86 356 214 515 1.77e-14 75.1
MS.gene24320.t1 MTR_8g033360 30.137 146 98 3 165 310 437 578 2.63e-14 74.7
MS.gene24320.t1 MTR_7g096160 26.441 295 185 14 64 345 356 631 1.24e-13 72.8
MS.gene24320.t1 MTR_6g013840 28.947 114 73 3 245 356 410 517 2.08e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24320.t1 AT3G50760 68.539 356 87 6 13 365 5 338 2.55e-178 498
MS.gene24320.t1 AT1G19300 76.061 330 76 2 40 367 23 351 1.10e-175 492
MS.gene24320.t1 AT4G02130 60.759 316 110 3 46 360 41 343 5.62e-145 414
MS.gene24320.t1 AT4G02130 60.759 316 110 3 46 360 41 343 5.62e-145 414
MS.gene24320.t1 AT4G02130 60.759 316 110 3 46 360 41 343 5.62e-145 414
MS.gene24320.t1 AT3G62660 67.138 283 89 2 76 358 77 355 4.19e-142 407
MS.gene24320.t1 AT1G02720 56.213 338 136 3 24 360 32 358 2.07e-139 400
MS.gene24320.t1 AT1G02720 56.213 338 136 3 24 360 32 358 2.07e-139 400
MS.gene24320.t1 AT1G70090 52.575 369 160 5 1 360 1 363 1.75e-138 399
MS.gene24320.t1 AT1G70090 52.575 369 160 5 1 360 1 363 1.75e-138 399
MS.gene24320.t1 AT1G13250 60.185 324 118 4 46 367 31 345 6.99e-138 396
MS.gene24320.t1 AT3G06260 57.729 317 115 3 46 361 46 344 1.53e-137 395
MS.gene24320.t1 AT1G24170 57.994 319 128 3 46 360 50 366 3.02e-136 394
MS.gene24320.t1 AT3G28340 57.595 316 124 4 48 361 44 351 1.62e-135 391
MS.gene24320.t1 AT1G18580 32.258 186 117 5 165 349 342 519 6.50e-21 94.4
MS.gene24320.t1 AT5G47780 31.667 180 110 5 165 341 420 589 1.19e-19 90.9
MS.gene24320.t1 AT5G54690 30.151 199 122 8 165 356 331 519 7.90e-19 88.2
MS.gene24320.t1 AT3G61130 30.198 202 125 7 165 363 477 665 9.86e-18 85.1
MS.gene24320.t1 AT4G38270 27.869 244 167 6 115 357 430 665 1.81e-17 84.3
MS.gene24320.t1 AT4G38270 27.869 244 167 6 115 357 434 669 2.02e-17 84.3
MS.gene24320.t1 AT3G25140 29.000 200 123 5 165 358 363 549 7.73e-17 82.4
MS.gene24320.t1 AT3G01040 25.287 261 172 9 110 356 233 484 7.98e-17 82.0
MS.gene24320.t1 AT2G30575 27.715 267 166 12 95 345 332 587 9.31e-17 82.0
MS.gene24320.t1 AT3G01040 27.778 198 128 7 165 356 329 517 1.70e-16 81.3
MS.gene24320.t1 AT3G01040 27.778 198 128 7 165 356 329 517 1.70e-16 81.3
MS.gene24320.t1 AT3G58790 30.000 200 122 8 165 356 204 393 1.70e-16 80.9
MS.gene24320.t1 AT3G58790 30.000 200 122 8 165 356 336 525 1.77e-16 81.3
MS.gene24320.t1 AT1G06780 27.586 261 162 9 95 339 311 560 2.08e-16 80.9
MS.gene24320.t1 AT1G06780 27.586 261 162 9 95 339 324 573 2.19e-16 80.9
MS.gene24320.t1 AT5G15470 26.768 198 130 7 165 356 329 517 6.31e-15 76.3
MS.gene24320.t1 AT2G46480 24.042 287 181 9 78 363 282 532 1.04e-14 75.9
MS.gene24320.t1 AT2G46480 24.042 287 181 9 78 363 295 545 1.06e-14 75.9
MS.gene24320.t1 AT2G20810 32.663 199 123 7 165 363 342 529 5.20e-13 70.5
MS.gene24320.t1 AT3G02350 29.167 192 126 6 165 356 365 546 6.96e-12 67.0
MS.gene24320.t1 AT2G46480 22.300 287 161 10 78 363 282 507 4.23e-11 64.7
MS.gene24320.t1 AT2G46480 22.300 287 161 10 78 363 295 520 5.39e-11 64.3

Find 72 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTCCTTTGGATGCTCTT+TGG 0.179612 1.1:+2584442 MS.gene24320:CDS
TTGTCCTTTGGATGCTCTTT+GGG 0.232558 1.1:+2584443 MS.gene24320:CDS
TTCGATGATTCAATCGTTTC+GGG 0.244627 1.1:+2583835 MS.gene24320:CDS
CAATTATGCAAGAAGCTATT+TGG 0.253341 1.1:+2583900 MS.gene24320:CDS
TTTACTTCACACCAACATTT+TGG 0.256817 1.1:+2584073 MS.gene24320:CDS
TCATGTTCGGTGTCTTCTTC+TGG 0.259724 1.1:-2583595 None:intergenic
AATCAAGAATCAAGAGAAAA+TGG 0.308067 1.1:-2584489 None:intergenic
TTACTTTAATACCGGGGTTA+TGG 0.328231 1.1:+2584140 MS.gene24320:CDS
TTTCGATGATTCAATCGTTT+CGG 0.340870 1.1:+2583834 MS.gene24320:CDS
TGCTAATAGACCTTGTCCTT+TGG 0.372206 1.1:+2584431 MS.gene24320:CDS
AAACCTTGTTACTTTAATAC+CGG 0.397438 1.1:+2584132 MS.gene24320:CDS
TCACTGATGGAGGCACAATT+TGG 0.401749 1.1:-2583571 None:intergenic
GTCTTCTTCTGGTTCACTGA+TGG 0.403834 1.1:-2583584 None:intergenic
TTGTTCCTGTGGATCATAGA+TGG 0.412121 1.1:+2584295 MS.gene24320:CDS
GTCCAGTGAGTTTATTGCAT+TGG 0.440935 1.1:+2584376 MS.gene24320:CDS
TGCAATAAACTCACTGGACC+TGG 0.441630 1.1:-2584372 None:intergenic
AATTGTAGACTTGGAATTTG+AGG 0.447752 1.1:-2583813 None:intergenic
AGGTAAACCATGGGTGAGAT+TGG 0.454495 1.1:+2584407 MS.gene24320:CDS
GCGAATGTTAAAGACAAAGA+TGG 0.456493 1.1:-2584102 None:intergenic
TTATTGCATTGGAGTGGAAA+AGG 0.458081 1.1:+2584387 MS.gene24320:CDS
TGTAAGAAAAGTTGTGTACT+TGG 0.460356 1.1:+2583942 MS.gene24320:CDS
TTTGGTGAGTTGTAGAATTG+AGG 0.465286 1.1:-2583553 None:intergenic
GACAGAGAGGATTGCAGCCA+TGG 0.467068 1.1:-2583692 None:intergenic
GGTGTCTGCAAAAGATCATA+AGG 0.476961 1.1:-2584468 None:intergenic
AGATGGAATCAACATGGTCT+TGG 0.481062 1.1:+2584312 MS.gene24320:CDS
TTGGTGAGTTGTAGAATTGA+GGG 0.481706 1.1:-2583552 None:intergenic
TGCAGGGGAAATTGTTCCTG+TGG 0.488544 1.1:+2584284 MS.gene24320:CDS
GTGGAGAAGTGAAGTGTTTG+AGG 0.488621 1.1:-2583764 None:intergenic
TTCTTCTGGTTCACTGATGG+AGG 0.490980 1.1:-2583581 None:intergenic
AATCATCGAAATTGTAGACT+TGG 0.491592 1.1:-2583822 None:intergenic
TAGACTTGGAATTTGAGGTA+AGG 0.496272 1.1:-2583808 None:intergenic
AACCTTGTTACTTTAATACC+GGG 0.498666 1.1:+2584133 MS.gene24320:CDS
CTTTGTCGAGATCTTCATCC+AGG 0.499442 1.1:+2584354 MS.gene24320:CDS
AGACTTGGAATTTGAGGTAA+GGG 0.499769 1.1:-2583807 None:intergenic
AACATATATCCGAGGTTCCA+TGG 0.527756 1.1:+2583675 MS.gene24320:CDS
AACAAAGATTGAGGAATGGA+TGG 0.528450 1.1:+2584200 MS.gene24320:CDS
CGATATCATCAACTAAGATA+AGG 0.533837 1.1:-2583972 None:intergenic
CGAATGTTAAAGACAAAGAT+GGG 0.534888 1.1:-2584101 None:intergenic
TGTTAAAGACAAAGATGGGT+TGG 0.537673 1.1:-2584097 None:intergenic
CTCCAATGCAATAAACTCAC+TGG 0.539210 1.1:-2584378 None:intergenic
AGGATGAGAATCTATGAACT+TGG 0.551100 1.1:+2584234 MS.gene24320:CDS
TTTGTGGACATGATGAGTGT+TGG 0.558842 1.1:-2583717 None:intergenic
GTGAGTTTATTGCATTGGAG+TGG 0.561039 1.1:+2584381 MS.gene24320:CDS
ATCAAGTGCTGATCGAATTG+AGG 0.561859 1.1:-2583869 None:intergenic
GTGGACATGATGAGTGTTGG+AGG 0.566977 1.1:-2583714 None:intergenic
AATGGATGGAATTACAAAAG+AGG 0.570341 1.1:+2584214 MS.gene24320:CDS
TGATTCCATCTATGATCCAC+AGG 0.576078 1.1:-2584300 None:intergenic
TGGAATCAACATGGTCTTGG+TGG 0.582134 1.1:+2584315 MS.gene24320:CDS
TTAGCATCCAATCTCACCCA+TGG 0.582810 1.1:-2584414 None:intergenic
TGGTGAGTTGTAGAATTGAG+GGG 0.584816 1.1:-2583551 None:intergenic
TAGCTTCTTGCATAATTGAG+TGG 0.588805 1.1:-2583895 None:intergenic
ATATGTGTCAGTGTCATGTT+CGG 0.593341 1.1:-2583608 None:intergenic
TGATTACACAACAAAGATTG+AGG 0.596745 1.1:+2584191 MS.gene24320:CDS
CAAAGAGCATCCAAAGGACA+AGG 0.605477 1.1:-2584441 None:intergenic
GGATTGCAGCCATGGAACCT+CGG 0.623683 1.1:-2583684 None:intergenic
TAGAGATTGAAGACACAAGA+GGG 0.627737 1.1:-2583458 None:intergenic
ACCCCGGTATTAAAGTAACA+AGG 0.631984 1.1:-2584135 None:intergenic
AAATTTGCATTGCAATACTC+CGG 0.633009 1.1:-2584045 None:intergenic
ATGAGTGTTGGAGGACAGAG+AGG 0.637026 1.1:-2583705 None:intergenic
GGAGTGGAAAAGGTAAACCA+TGG 0.642822 1.1:+2584397 MS.gene24320:CDS
CTCTGAGGAAGCAGTCCATG+TGG 0.643941 1.1:+2583636 MS.gene24320:CDS
TAGATCAATTACCATAACCC+CGG 0.646191 1.1:-2584151 None:intergenic
GATGGGTTGGACCAAAATGT+TGG 0.647131 1.1:-2584084 None:intergenic
ACACAACAAAGATTGAGGAA+TGG 0.651197 1.1:+2584196 MS.gene24320:CDS
TGTCTAATGTCATTGCCACA+TGG 0.655175 1.1:-2583651 None:intergenic
ATAGAGATTGAAGACACAAG+AGG 0.656250 1.1:-2583459 None:intergenic
GAGTGGAAAAGGTAAACCAT+GGG 0.662161 1.1:+2584398 MS.gene24320:CDS
ACCTTGTTACTTTAATACCG+GGG 0.664037 1.1:+2584134 MS.gene24320:CDS
GGTGCCCAAAGAGCATCCAA+AGG 0.694592 1.1:-2584447 None:intergenic
GATCATAGATGGAATCAACA+TGG 0.708687 1.1:+2584306 MS.gene24320:CDS
TTAGACACAACATATATCCG+AGG 0.716181 1.1:+2583667 MS.gene24320:CDS
TGACACATATATCTGCTCTG+AGG 0.739745 1.1:+2583621 MS.gene24320:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAATAAGAGTTTTGGTTT+TGG - chr1.1:2583424-2583443 None:intergenic 20.0%
!!! AATTGTTTTTCTTGTTTTTG+AGG - chr1.1:2583509-2583528 None:intergenic 20.0%
!!! AGAAAGAAAAATAAGAGTTT+TGG - chr1.1:2583430-2583449 None:intergenic 20.0%
! TGGTAATTGATCTAGAAAAA+TGG + chr1.1:2584160-2584179 MS.gene24320:CDS 25.0%
!! AAGTGAAAAATGATGTTTTG+TGG - chr1.1:2583736-2583755 None:intergenic 25.0%
!! ACAACTTTTCTTACACATAA+TGG - chr1.1:2583937-2583956 None:intergenic 25.0%
!!! AAACCTTGTTACTTTAATAC+CGG + chr1.1:2584132-2584151 MS.gene24320:CDS 25.0%
!!! TGTTTTTGAGGCATTTTTTT+TGG - chr1.1:2583497-2583516 None:intergenic 25.0%
AAATTTGCATTGCAATACTC+CGG - chr1.1:2584048-2584067 None:intergenic 30.0%
AATCATCGAAATTGTAGACT+TGG - chr1.1:2583825-2583844 None:intergenic 30.0%
AATGGATGGAATTACAAAAG+AGG + chr1.1:2584214-2584233 MS.gene24320:CDS 30.0%
AATTGTAGACTTGGAATTTG+AGG - chr1.1:2583816-2583835 None:intergenic 30.0%
CAATTATGCAAGAAGCTATT+TGG + chr1.1:2583900-2583919 MS.gene24320:CDS 30.0%
CGAATGTTAAAGACAAAGAT+GGG - chr1.1:2584104-2584123 None:intergenic 30.0%
CGATATCATCAACTAAGATA+AGG - chr1.1:2583975-2583994 None:intergenic 30.0%
TGATTACACAACAAAGATTG+AGG + chr1.1:2584191-2584210 MS.gene24320:CDS 30.0%
TGTAAGAAAAGTTGTGTACT+TGG + chr1.1:2583942-2583961 MS.gene24320:CDS 30.0%
TTTCGATGATTCAATCGTTT+CGG + chr1.1:2583834-2583853 MS.gene24320:CDS 30.0%
! TTTACTTCACACCAACATTT+TGG + chr1.1:2584073-2584092 MS.gene24320:CDS 30.0%
!! AACCTTGTTACTTTAATACC+GGG + chr1.1:2584133-2584152 MS.gene24320:CDS 30.0%
!!! AGGGAATGATTTTGAGATTA+TGG - chr1.1:2583791-2583810 None:intergenic 30.0%
!!! ATGATTTTGAGATTATGGAG+TGG - chr1.1:2583786-2583805 None:intergenic 30.0%
!!! CTTTTTTGCTTGTTTTTGCA+GGG + chr1.1:2584268-2584287 MS.gene24320:CDS 30.0%
!!! TGCCAAAACTGCATTTTTTT+CGG - chr1.1:2584022-2584041 None:intergenic 30.0%
!!! TTTTTTGCTTGTTTTTGCAG+GGG + chr1.1:2584269-2584288 MS.gene24320:CDS 30.0%
AACAAAGATTGAGGAATGGA+TGG + chr1.1:2584200-2584219 MS.gene24320:CDS 35.0%
ACACAACAAAGATTGAGGAA+TGG + chr1.1:2584196-2584215 MS.gene24320:CDS 35.0%
AGGATGAGAATCTATGAACT+TGG + chr1.1:2584234-2584253 MS.gene24320:CDS 35.0%
ATAGAGATTGAAGACACAAG+AGG - chr1.1:2583462-2583481 None:intergenic 35.0%
ATATGTGTCAGTGTCATGTT+CGG - chr1.1:2583611-2583630 None:intergenic 35.0%
CCTGCAAAAACAAGCAAAAA+AGG - chr1.1:2584270-2584289 None:intergenic 35.0%
GATCATAGATGGAATCAACA+TGG + chr1.1:2584306-2584325 MS.gene24320:CDS 35.0%
GCAAAAACAAGCAAAAAAGG+TGG - chr1.1:2584267-2584286 None:intergenic 35.0%
GCGAATGTTAAAGACAAAGA+TGG - chr1.1:2584105-2584124 None:intergenic 35.0%
TAGAGATTGAAGACACAAGA+GGG - chr1.1:2583461-2583480 None:intergenic 35.0%
TAGATCAATTACCATAACCC+CGG - chr1.1:2584154-2584173 None:intergenic 35.0%
TAGCTTCTTGCATAATTGAG+TGG - chr1.1:2583898-2583917 None:intergenic 35.0%
TGTTAAAGACAAAGATGGGT+TGG - chr1.1:2584100-2584119 None:intergenic 35.0%
TTACTTTAATACCGGGGTTA+TGG + chr1.1:2584140-2584159 MS.gene24320:CDS 35.0%
TTAGACACAACATATATCCG+AGG + chr1.1:2583667-2583686 MS.gene24320:CDS 35.0%
TTATTGCATTGGAGTGGAAA+AGG + chr1.1:2584387-2584406 MS.gene24320:CDS 35.0%
TTCGATGATTCAATCGTTTC+GGG + chr1.1:2583835-2583854 MS.gene24320:CDS 35.0%
TTGGTGAGTTGTAGAATTGA+GGG - chr1.1:2583555-2583574 None:intergenic 35.0%
TTTGGTGAGTTGTAGAATTG+AGG - chr1.1:2583556-2583575 None:intergenic 35.0%
! AGACTTGGAATTTGAGGTAA+GGG - chr1.1:2583810-2583829 None:intergenic 35.0%
! AGCCGAAAAAAATGCAGTTT+TGG + chr1.1:2584017-2584036 MS.gene24320:CDS 35.0%
! TAGACTTGGAATTTGAGGTA+AGG - chr1.1:2583811-2583830 None:intergenic 35.0%
!! ACCTTGTTACTTTAATACCG+GGG + chr1.1:2584134-2584153 MS.gene24320:CDS 35.0%
!!! ACTGCATTTTTTTCGGCTAA+TGG - chr1.1:2584015-2584034 None:intergenic 35.0%
!!! CCTTTTTTGCTTGTTTTTGC+AGG + chr1.1:2584267-2584286 MS.gene24320:CDS 35.0%
!!! TGAGGCATTTTTTTTGGTGT+TGG - chr1.1:2583491-2583510 None:intergenic 35.0%
AACATATATCCGAGGTTCCA+TGG + chr1.1:2583675-2583694 MS.gene24320:CDS 40.0%
ACCCCGGTATTAAAGTAACA+AGG - chr1.1:2584138-2584157 None:intergenic 40.0%
AGATGGAATCAACATGGTCT+TGG + chr1.1:2584312-2584331 MS.gene24320:CDS 40.0%
CTCCAATGCAATAAACTCAC+TGG - chr1.1:2584381-2584400 None:intergenic 40.0%
GAGTGGAAAAGGTAAACCAT+GGG + chr1.1:2584398-2584417 MS.gene24320:CDS 40.0%
GGTGTCTGCAAAAGATCATA+AGG - chr1.1:2584471-2584490 None:intergenic 40.0%
GTCCAGTGAGTTTATTGCAT+TGG + chr1.1:2584376-2584395 MS.gene24320:CDS 40.0%
GTGAGTTTATTGCATTGGAG+TGG + chr1.1:2584381-2584400 MS.gene24320:CDS 40.0%
TGACACATATATCTGCTCTG+AGG + chr1.1:2583621-2583640 MS.gene24320:CDS 40.0%
TGATTCCATCTATGATCCAC+AGG - chr1.1:2584303-2584322 None:intergenic 40.0%
TGCTAATAGACCTTGTCCTT+TGG + chr1.1:2584431-2584450 MS.gene24320:CDS 40.0%
TGGTGAGTTGTAGAATTGAG+GGG - chr1.1:2583554-2583573 None:intergenic 40.0%
TGTCTAATGTCATTGCCACA+TGG - chr1.1:2583654-2583673 None:intergenic 40.0%
TTGTCCTTTGGATGCTCTTT+GGG + chr1.1:2584443-2584462 MS.gene24320:CDS 40.0%
TTGTTCCTGTGGATCATAGA+TGG + chr1.1:2584295-2584314 MS.gene24320:CDS 40.0%
TTTGTGGACATGATGAGTGT+TGG - chr1.1:2583720-2583739 None:intergenic 40.0%
!! ATCAAGTGCTGATCGAATTG+AGG - chr1.1:2583872-2583891 None:intergenic 40.0%
!!! CTTGGTGGTGATAATTTTCG+AGG + chr1.1:2584330-2584349 MS.gene24320:CDS 40.0%
!!! GGCATTTTTTTTGGTGTTGG+AGG - chr1.1:2583488-2583507 None:intergenic 40.0%
AGGTAAACCATGGGTGAGAT+TGG + chr1.1:2584407-2584426 MS.gene24320:CDS 45.0%
CTTGTCCTTTGGATGCTCTT+TGG + chr1.1:2584442-2584461 MS.gene24320:CDS 45.0%
CTTTGTCGAGATCTTCATCC+AGG + chr1.1:2584354-2584373 MS.gene24320:CDS 45.0%
GATCTAGAAAAATGGCGCGA+CGG + chr1.1:2584168-2584187 MS.gene24320:CDS 45.0%
GGAGTGGAAAAGGTAAACCA+TGG + chr1.1:2584397-2584416 MS.gene24320:CDS 45.0%
TGCAATAAACTCACTGGACC+TGG - chr1.1:2584375-2584394 None:intergenic 45.0%
TTAGCATCCAATCTCACCCA+TGG - chr1.1:2584417-2584436 None:intergenic 45.0%
! GTGGAGAAGTGAAGTGTTTG+AGG - chr1.1:2583767-2583786 None:intergenic 45.0%
! TCACTGATGGAGGCACAATT+TGG - chr1.1:2583574-2583593 None:intergenic 45.0%
! TGGAATCAACATGGTCTTGG+TGG + chr1.1:2584315-2584334 MS.gene24320:CDS 45.0%
!! AAATGCAGTTTTGGCAGCAC+CGG + chr1.1:2584026-2584045 MS.gene24320:CDS 45.0%
!! CAAAGAGCATCCAAAGGACA+AGG - chr1.1:2584444-2584463 None:intergenic 45.0%
!! GATGGGTTGGACCAAAATGT+TGG - chr1.1:2584087-2584106 None:intergenic 45.0%
!! GTCTTCTTCTGGTTCACTGA+TGG - chr1.1:2583587-2583606 None:intergenic 45.0%
!! TCATGTTCGGTGTCTTCTTC+TGG - chr1.1:2583598-2583617 None:intergenic 45.0%
!! TTCTTCTGGTTCACTGATGG+AGG - chr1.1:2583584-2583603 None:intergenic 45.0%
TGCAGGGGAAATTGTTCCTG+TGG + chr1.1:2584284-2584303 MS.gene24320:CDS 50.0%
!! ATGAGTGTTGGAGGACAGAG+AGG - chr1.1:2583708-2583727 None:intergenic 50.0%
!! GTGGACATGATGAGTGTTGG+AGG - chr1.1:2583717-2583736 None:intergenic 50.0%
GACAGAGAGGATTGCAGCCA+TGG - chr1.1:2583695-2583714 None:intergenic 55.0%
GGATTGCAGCCATGGAACCT+CGG - chr1.1:2583687-2583706 None:intergenic 55.0%
! CTCTGAGGAAGCAGTCCATG+TGG + chr1.1:2583636-2583655 MS.gene24320:CDS 55.0%
!! GGTGCCCAAAGAGCATCCAA+AGG - chr1.1:2584450-2584469 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 2583406 2584509 2583406 ID=MS.gene24320
chr1.1 mRNA 2583406 2584509 2583406 ID=MS.gene24320.t1;Parent=MS.gene24320
chr1.1 exon 2583406 2584509 2583406 ID=MS.gene24320.t1.exon1;Parent=MS.gene24320.t1
chr1.1 CDS 2583406 2584509 2583406 ID=cds.MS.gene24320.t1;Parent=MS.gene24320.t1
Gene Sequence

>MS.gene24320

ATGAAGATCAATTTTCCAAAACCAAAACTCTTATTTTTCTTTCTCATTCTCTCCCTCTTGTGTCTTCAATCTCTATCATCCTCCAACACCAAAAAAAATGCCTCAAAAACAAGAAAAACAATTATCAATGATCAATTCAAAGAAGCCCCTCAATTCTACAACTCACCAAATTGTGCCTCCATCAGTGAACCAGAAGAAGACACCGAACATGACACTGACACATATATCTGCTCTGAGGAAGCAGTCCATGTGGCAATGACATTAGACACAACATATATCCGAGGTTCCATGGCTGCAATCCTCTCTGTCCTCCAACACTCATCATGTCCACAAAACATCATTTTTCACTTTGTTTGTTCCTCAAACACTTCACTTCTCCACTCCATAATCTCAAAATCATTCCCTTACCTCAAATTCCAAGTCTACAATTTCGATGATTCAATCGTTTCGGGATTAATCTCAACCTCAATTCGATCAGCACTTGATTGTCCACTCAATTATGCAAGAAGCTATTTGGCAAATCTAATTCCATTATGTGTAAGAAAAGTTGTGTACTTGGATTCAGACCTTATCTTAGTTGATGATATCGCAAAACTTGCATCAACACCATTAGCCGAAAAAAATGCAGTTTTGGCAGCACCGGAGTATTGCAATGCAAATTTCACATTTTACTTCACACCAACATTTTGGTCCAACCCATCTTTGTCTTTAACATTCGCGAATCGAAAACCTTGTTACTTTAATACCGGGGTTATGGTAATTGATCTAGAAAAATGGCGCGACGGTGATTACACAACAAAGATTGAGGAATGGATGGAATTACAAAAGAGGATGAGAATCTATGAACTTGGTTCATTGCCACCTTTTTTGCTTGTTTTTGCAGGGGAAATTGTTCCTGTGGATCATAGATGGAATCAACATGGTCTTGGTGGTGATAATTTTCGAGGTCTTTGTCGAGATCTTCATCCAGGTCCAGTGAGTTTATTGCATTGGAGTGGAAAAGGTAAACCATGGGTGAGATTGGATGCTAATAGACCTTGTCCTTTGGATGCTCTTTGGGCACCTTATGATCTTTTGCAGACACCATTTTCTCTTGATTCTTGA
Protein sequence

>MS.gene24320.t1

MKINFPKPKLLFFFLILSLLCLQSLSSSNTKKNASKTRKTIINDQFKEAPQFYNSPNCASISEPEEDTEHDTDTYICSEEAVHVAMTLDTTYIRGSMAAILSVLQHSSCPQNIIFHFVCSSNTSLLHSIISKSFPYLKFQVYNFDDSIVSGLISTSIRSALDCPLNYARSYLANLIPLCVRKVVYLDSDLILVDDIAKLASTPLAEKNAVLAAPEYCNANFTFYFTPTFWSNPSLSLTFANRKPCYFNTGVMVIDLEKWRDGDYTTKIEEWMELQKRMRIYELGSLPPFLLVFAGEIVPVDHRWNQHGLGGDNFRGLCRDLHPGPVSLLHWSGKGKPWVRLDANRPCPLDALWAPYDLLQTPFSLDS