Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060376.t1 | XP_013458228.1 | 98.6 | 347 | 5 | 0 | 1 | 347 | 1 | 347 | 2.60E-201 | 711.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060376.t1 | Q9M8J2 | 75.1 | 342 | 76 | 5 | 14 | 347 | 11 | 351 | 7.2e-152 | 538.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060376.t1 | A0A072UQZ6 | 98.6 | 347 | 5 | 0 | 1 | 347 | 1 | 347 | 1.9e-201 | 711.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene060376 | MS.gene24320 | PPI |
| MS.gene062721 | MS.gene060376 | PPI |
| MS.gene27350 | MS.gene060376 | PPI |
| MS.gene69122 | MS.gene060376 | PPI |
| MS.gene060376 | MS.gene014546 | PPI |
| MS.gene060376 | MS.gene047738 | PPI |
| MS.gene26925 | MS.gene060376 | PPI |
| MS.gene060376 | MS.gene42362 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060376.t1 | MTR_4g118030 | 98.559 | 347 | 5 | 0 | 1 | 347 | 1 | 347 | 0.0 | 714 |
| MS.gene060376.t1 | MTR_7g094230 | 74.837 | 306 | 73 | 2 | 42 | 347 | 51 | 352 | 1.26e-174 | 489 |
| MS.gene060376.t1 | MTR_1g089820 | 73.065 | 323 | 83 | 2 | 25 | 347 | 37 | 355 | 3.56e-171 | 480 |
| MS.gene060376.t1 | MTR_3g092310 | 61.424 | 337 | 120 | 4 | 8 | 341 | 6 | 335 | 5.55e-155 | 439 |
| MS.gene060376.t1 | MTR_7g109210 | 54.651 | 344 | 144 | 5 | 10 | 344 | 17 | 357 | 2.86e-137 | 394 |
| MS.gene060376.t1 | MTR_5g036580 | 54.571 | 361 | 134 | 6 | 10 | 344 | 9 | 365 | 7.98e-137 | 394 |
| MS.gene060376.t1 | MTR_1g021855 | 58.044 | 317 | 114 | 4 | 42 | 340 | 46 | 361 | 6.56e-133 | 384 |
| MS.gene060376.t1 | MTR_3g111600 | 61.429 | 280 | 106 | 2 | 66 | 344 | 1 | 279 | 3.19e-130 | 374 |
| MS.gene060376.t1 | MTR_8g078240 | 56.090 | 312 | 123 | 4 | 42 | 340 | 29 | 339 | 9.77e-130 | 375 |
| MS.gene060376.t1 | MTR_5g019220 | 54.434 | 327 | 121 | 4 | 41 | 340 | 36 | 361 | 4.13e-127 | 369 |
| MS.gene060376.t1 | MTR_6g008900 | 53.651 | 315 | 127 | 5 | 42 | 338 | 47 | 360 | 6.55e-126 | 366 |
| MS.gene060376.t1 | MTR_4g071620 | 53.651 | 315 | 127 | 5 | 42 | 338 | 47 | 360 | 6.55e-126 | 366 |
| MS.gene060376.t1 | MTR_4g133570 | 30.729 | 192 | 122 | 5 | 135 | 320 | 251 | 437 | 5.18e-25 | 105 |
| MS.gene060376.t1 | MTR_7g012370 | 31.694 | 183 | 108 | 6 | 145 | 318 | 334 | 508 | 1.65e-24 | 104 |
| MS.gene060376.t1 | MTR_2g082650 | 29.255 | 188 | 110 | 6 | 145 | 320 | 209 | 385 | 6.13e-24 | 102 |
| MS.gene060376.t1 | MTR_4g109890 | 32.768 | 177 | 111 | 5 | 145 | 320 | 340 | 509 | 1.21e-23 | 102 |
| MS.gene060376.t1 | MTR_2g082650 | 29.255 | 188 | 110 | 6 | 145 | 320 | 338 | 514 | 1.50e-23 | 101 |
| MS.gene060376.t1 | MTR_6g016380 | 30.890 | 191 | 123 | 5 | 145 | 335 | 359 | 540 | 3.33e-23 | 100 |
| MS.gene060376.t1 | MTR_2g461670 | 31.073 | 177 | 114 | 5 | 145 | 320 | 395 | 564 | 5.37e-23 | 100 |
| MS.gene060376.t1 | MTR_7g055600 | 32.124 | 193 | 118 | 6 | 145 | 335 | 238 | 419 | 6.27e-23 | 99.4 |
| MS.gene060376.t1 | MTR_7g057530 | 32.124 | 193 | 118 | 6 | 145 | 335 | 238 | 419 | 6.27e-23 | 99.4 |
| MS.gene060376.t1 | MTR_3g452360 | 29.319 | 191 | 109 | 7 | 145 | 320 | 175 | 354 | 3.58e-22 | 96.7 |
| MS.gene060376.t1 | MTR_3g452360 | 29.319 | 191 | 109 | 7 | 145 | 320 | 324 | 503 | 6.35e-22 | 97.1 |
| MS.gene060376.t1 | MTR_8g092000 | 30.508 | 177 | 115 | 5 | 145 | 320 | 384 | 553 | 1.77e-21 | 95.9 |
| MS.gene060376.t1 | MTR_2g027740 | 31.073 | 177 | 114 | 5 | 145 | 320 | 471 | 640 | 2.10e-21 | 95.9 |
| MS.gene060376.t1 | MTR_2g015550 | 27.839 | 273 | 157 | 10 | 62 | 331 | 285 | 520 | 3.63e-21 | 94.7 |
| MS.gene060376.t1 | MTR_7g075840 | 26.482 | 253 | 151 | 7 | 69 | 320 | 419 | 637 | 1.57e-19 | 90.1 |
| MS.gene060376.t1 | MTR_8g033360 | 27.193 | 228 | 138 | 6 | 62 | 289 | 379 | 578 | 3.64e-19 | 89.0 |
| MS.gene060376.t1 | MTR_3g107930 | 24.710 | 259 | 164 | 7 | 62 | 320 | 334 | 561 | 8.11e-19 | 87.8 |
| MS.gene060376.t1 | MTR_3g107930 | 24.710 | 259 | 164 | 7 | 62 | 320 | 411 | 638 | 1.04e-18 | 87.8 |
| MS.gene060376.t1 | MTR_6g013840 | 32.418 | 182 | 112 | 5 | 145 | 320 | 329 | 505 | 5.78e-18 | 85.1 |
| MS.gene060376.t1 | MTR_7g096160 | 29.213 | 178 | 115 | 6 | 145 | 318 | 455 | 625 | 9.01e-16 | 79.0 |
| MS.gene060376.t1 | MTR_2g006790 | 29.032 | 217 | 118 | 9 | 119 | 331 | 312 | 496 | 1.16e-13 | 72.0 |
| MS.gene060376.t1 | MTR_7g012370 | 32.432 | 111 | 68 | 3 | 145 | 250 | 334 | 442 | 5.39e-13 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060376.t1 | AT3G06260 | 76.923 | 325 | 71 | 4 | 26 | 347 | 28 | 351 | 0.0 | 533 |
| MS.gene060376.t1 | AT4G02130 | 56.805 | 338 | 132 | 5 | 10 | 341 | 16 | 345 | 2.11e-141 | 404 |
| MS.gene060376.t1 | AT4G02130 | 56.805 | 338 | 132 | 5 | 10 | 341 | 16 | 345 | 2.11e-141 | 404 |
| MS.gene060376.t1 | AT4G02130 | 56.805 | 338 | 132 | 5 | 10 | 341 | 16 | 345 | 2.11e-141 | 404 |
| MS.gene060376.t1 | AT1G13250 | 62.154 | 325 | 112 | 3 | 27 | 341 | 16 | 339 | 3.53e-141 | 404 |
| MS.gene060376.t1 | AT1G70090 | 60.252 | 317 | 109 | 4 | 38 | 338 | 47 | 362 | 3.36e-140 | 403 |
| MS.gene060376.t1 | AT1G70090 | 60.252 | 317 | 109 | 4 | 38 | 338 | 47 | 362 | 3.36e-140 | 403 |
| MS.gene060376.t1 | AT1G24170 | 54.795 | 365 | 128 | 7 | 8 | 339 | 6 | 366 | 4.43e-140 | 403 |
| MS.gene060376.t1 | AT3G62660 | 56.395 | 344 | 132 | 5 | 10 | 338 | 16 | 356 | 3.49e-138 | 397 |
| MS.gene060376.t1 | AT1G02720 | 58.599 | 314 | 121 | 3 | 36 | 341 | 48 | 360 | 2.46e-137 | 395 |
| MS.gene060376.t1 | AT1G02720 | 58.599 | 314 | 121 | 3 | 36 | 341 | 48 | 360 | 2.46e-137 | 395 |
| MS.gene060376.t1 | AT3G28340 | 55.901 | 322 | 128 | 4 | 36 | 346 | 39 | 357 | 9.49e-137 | 393 |
| MS.gene060376.t1 | AT3G50760 | 56.604 | 318 | 125 | 4 | 42 | 347 | 25 | 341 | 3.13e-129 | 373 |
| MS.gene060376.t1 | AT1G19300 | 58.176 | 318 | 113 | 4 | 42 | 340 | 29 | 345 | 8.94e-126 | 365 |
| MS.gene060376.t1 | AT3G01040 | 31.868 | 182 | 113 | 5 | 145 | 320 | 296 | 472 | 2.10e-26 | 109 |
| MS.gene060376.t1 | AT3G01040 | 31.868 | 182 | 113 | 5 | 145 | 320 | 329 | 505 | 3.04e-26 | 109 |
| MS.gene060376.t1 | AT3G01040 | 31.868 | 182 | 113 | 5 | 145 | 320 | 329 | 505 | 3.04e-26 | 109 |
| MS.gene060376.t1 | AT5G15470 | 32.973 | 185 | 107 | 6 | 145 | 320 | 329 | 505 | 2.89e-25 | 107 |
| MS.gene060376.t1 | AT3G25140 | 32.828 | 198 | 117 | 7 | 145 | 337 | 363 | 549 | 1.86e-24 | 104 |
| MS.gene060376.t1 | AT5G54690 | 30.556 | 180 | 114 | 5 | 145 | 318 | 331 | 505 | 2.59e-24 | 103 |
| MS.gene060376.t1 | AT1G18580 | 32.203 | 177 | 112 | 5 | 145 | 320 | 342 | 511 | 5.79e-24 | 103 |
| MS.gene060376.t1 | AT5G47780 | 32.386 | 176 | 113 | 5 | 145 | 320 | 420 | 589 | 6.97e-24 | 103 |
| MS.gene060376.t1 | AT4G38270 | 32.961 | 179 | 108 | 5 | 145 | 320 | 484 | 653 | 2.08e-22 | 99.0 |
| MS.gene060376.t1 | AT4G38270 | 32.961 | 179 | 108 | 5 | 145 | 320 | 480 | 649 | 2.29e-22 | 98.6 |
| MS.gene060376.t1 | AT3G58790 | 30.000 | 200 | 121 | 8 | 145 | 335 | 204 | 393 | 5.12e-21 | 93.6 |
| MS.gene060376.t1 | AT3G58790 | 30.000 | 200 | 121 | 8 | 145 | 335 | 336 | 525 | 6.30e-21 | 94.4 |
| MS.gene060376.t1 | AT1G06780 | 33.333 | 177 | 109 | 5 | 145 | 318 | 403 | 573 | 3.40e-20 | 92.0 |
| MS.gene060376.t1 | AT1G06780 | 33.333 | 177 | 109 | 5 | 145 | 318 | 390 | 560 | 4.16e-20 | 92.0 |
| MS.gene060376.t1 | AT3G61130 | 29.379 | 177 | 117 | 5 | 145 | 320 | 477 | 646 | 3.80e-19 | 89.0 |
| MS.gene060376.t1 | AT2G30575 | 31.844 | 179 | 113 | 6 | 145 | 320 | 411 | 583 | 1.53e-18 | 87.0 |
| MS.gene060376.t1 | AT3G02350 | 29.319 | 191 | 126 | 5 | 145 | 335 | 365 | 546 | 1.34e-17 | 84.3 |
| MS.gene060376.t1 | AT2G46480 | 25.940 | 266 | 160 | 9 | 62 | 324 | 286 | 517 | 2.10e-16 | 80.5 |
| MS.gene060376.t1 | AT2G46480 | 25.954 | 262 | 157 | 9 | 62 | 320 | 299 | 526 | 2.90e-16 | 80.1 |
| MS.gene060376.t1 | AT2G20810 | 29.091 | 275 | 150 | 11 | 69 | 340 | 295 | 527 | 1.24e-15 | 78.2 |
Find 95 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTATTTCACAAAACAATTT+TGG | 0.231657 | 8.4:+13052482 | MS.gene060376:CDS |
| CTCCAATGCAATAAACTAAT+TGG | 0.232412 | 8.4:-13052790 | None:intergenic |
| CATGCATTGTGGACCATCTA+TGG | 0.254614 | 8.4:+13052854 | MS.gene060376:CDS |
| ATCCAAGAGGATATATCATT+TGG | 0.280778 | 8.4:+13052645 | MS.gene060376:CDS |
| TTTAAGACAGGATGGTCTTC+AGG | 0.316583 | 8.4:+13051870 | None:intergenic |
| TTTATGCTTGTGAATAGTTC+AGG | 0.327122 | 8.4:-13052190 | None:intergenic |
| TGGTGATGGATGTGGATAAA+TGG | 0.329278 | 8.4:+13052569 | MS.gene060376:CDS |
| AGATGGAACCAACATGGTTT+AGG | 0.357389 | 8.4:+13052724 | MS.gene060376:CDS |
| AAATCTGTATGACAATATTC+TGG | 0.369299 | 8.4:-13052454 | None:intergenic |
| CTTGATGTTAATTACCTTCG+TGG | 0.380377 | 8.4:+13052082 | MS.gene060376:CDS |
| TTAAAGCAGTTGATCATAGA+TGG | 0.380750 | 8.4:+13052707 | MS.gene060376:CDS |
| TAATGGGGTGGAGGAAGGAC+AGG | 0.388988 | 8.4:-13051931 | None:intergenic |
| GTTCTCGCAAAGACGTTTGA+TGG | 0.395785 | 8.4:+13052514 | MS.gene060376:CDS |
| TCCTCGCCGTTCCGGAAAGC+GGG | 0.396369 | 8.4:-13052016 | None:intergenic |
| CATGGTTGAGGTTGGATTCA+AGG | 0.396771 | 8.4:+13052827 | MS.gene060376:CDS |
| AGCCACATTCCTCGCCGTTC+CGG | 0.410897 | 8.4:-13052024 | None:intergenic |
| GTCCAATTAGTTTATTGCAT+TGG | 0.412346 | 8.4:+13052788 | MS.gene060376:CDS |
| TTGATGATATAGCTAAGCTT+TGG | 0.412649 | 8.4:+13052398 | MS.gene060376:CDS |
| TTTAATTCTGATCGTGTTCG+CGG | 0.414607 | 8.4:+13052250 | MS.gene060376:CDS |
| CGTGTAGTTGTCATACTCCT+TGG | 0.415246 | 8.4:+13051905 | MS.gene060376:CDS |
| TCAAACGTCTTTGCGAGAAC+CGG | 0.418289 | 8.4:-13052511 | None:intergenic |
| CCAAGACGGATAGAGGTAAT+GGG | 0.421902 | 8.4:-13051947 | None:intergenic |
| TGATGATATAGCTAAGCTTT+GGG | 0.428107 | 8.4:+13052399 | MS.gene060376:CDS |
| GGAGGAAGGACAGGAGGCCA+AGG | 0.431784 | 8.4:-13051922 | None:intergenic |
| GGGCTCCTTACGATTTGTAT+AGG | 0.441558 | 8.4:+13052875 | MS.gene060376:CDS |
| AGAGGTAATGGGGTGGAGGA+AGG | 0.442243 | 8.4:-13051936 | None:intergenic |
| GCCAAGACGGATAGAGGTAA+TGG | 0.446240 | 8.4:-13051948 | None:intergenic |
| ATGAGCAGAAAGGAAGTAGA+AGG | 0.446739 | 8.4:-13052161 | None:intergenic |
| TTATTTCAACACAGGTGTAA+TGG | 0.450858 | 8.4:+13052549 | MS.gene060376:CDS |
| TTATTGCATTGGAGTGGAAA+AGG | 0.458081 | 8.4:+13052799 | MS.gene060376:CDS |
| CAGAAGAGGGTAGGCGGACA+AGG | 0.465642 | 8.4:-13051970 | None:intergenic |
| TCCAAGAGGATATATCATTT+GGG | 0.465682 | 8.4:+13052646 | MS.gene060376:CDS |
| GGATAGAGGTAATGGGGTGG+AGG | 0.471735 | 8.4:-13051940 | None:intergenic |
| AATCGTAAGGAGCCCATAGA+TGG | 0.474933 | 8.4:-13052867 | None:intergenic |
| AAATCGTCATGTTTAGATAA+GGG | 0.479874 | 8.4:-13052222 | None:intergenic |
| CACGAAGGTAATTAACATCA+AGG | 0.480331 | 8.4:-13052081 | None:intergenic |
| GACAAGGCCAAGACGGATAG+AGG | 0.483676 | 8.4:-13051954 | None:intergenic |
| CCCATTACCTCTATCCGTCT+TGG | 0.486806 | 8.4:+13051947 | MS.gene060376:CDS |
| ATACAGAGAAAGTTGAAGAA+TGG | 0.487108 | 8.4:+13052605 | MS.gene060376:CDS |
| TAAATCGTCATGTTTAGATA+AGG | 0.495139 | 8.4:-13052223 | None:intergenic |
| AAAGTCAATTAGAAAAGCAT+TGG | 0.497024 | 8.4:+13052282 | MS.gene060376:CDS |
| TTCCTCGCCGTTCCGGAAAG+CGG | 0.498683 | 8.4:-13052017 | None:intergenic |
| ACCCAAATGATATATCCTCT+TGG | 0.500116 | 8.4:-13052647 | None:intergenic |
| TATATCCTTGCATAATTCAA+AGG | 0.509392 | 8.4:-13052310 | None:intergenic |
| TGCAATAAACTAATTGGACC+AGG | 0.513743 | 8.4:-13052784 | None:intergenic |
| TTAGGTGGAGATAACATTGA+AGG | 0.533787 | 8.4:+13052742 | MS.gene060376:CDS |
| CATGGACAACACTGCAGCCA+TGG | 0.535328 | 8.4:-13052107 | None:intergenic |
| AGGTGTAATGGTGATGGATG+TGG | 0.537826 | 8.4:+13052561 | MS.gene060376:CDS |
| AGGTAAACCATGGTTGAGGT+TGG | 0.540509 | 8.4:+13052819 | MS.gene060376:CDS |
| CTTGAATCCAACCTCAACCA+TGG | 0.544223 | 8.4:-13052826 | None:intergenic |
| ATTAGTTTATTGCATTGGAG+TGG | 0.546400 | 8.4:+13052793 | MS.gene060376:CDS |
| TTCCGGAACGGCGAGGAATG+TGG | 0.547862 | 8.4:+13052022 | MS.gene060376:CDS |
| ATCAACCTTTGAATTATGCA+AGG | 0.548743 | 8.4:+13052305 | MS.gene060376:CDS |
| CAAACGTCTTTGCGAGAACC+GGG | 0.553347 | 8.4:-13052510 | None:intergenic |
| ATGCATTGTGGACCATCTAT+GGG | 0.557021 | 8.4:+13052855 | MS.gene060376:CDS |
| CCTCGCCGTTCCGGAAAGCG+GGG | 0.557081 | 8.4:-13052015 | None:intergenic |
| GTAATTAACATCAAGGGTCA+TGG | 0.557438 | 8.4:-13052074 | None:intergenic |
| TTTAGATTCTGACCTTGTTG+TGG | 0.558089 | 8.4:+13052375 | MS.gene060376:CDS |
| GGAGGTCAGAAGAGGGTAGG+CGG | 0.560759 | 8.4:-13051976 | None:intergenic |
| AGAGAAAGTTGAAGAATGGA+TGG | 0.562628 | 8.4:+13052609 | MS.gene060376:CDS |
| CGTCATGTTTAGATAAGGGA+AGG | 0.565903 | 8.4:-13052218 | None:intergenic |
| ATTTATACGATCAGCTTCTG+TGG | 0.568749 | 8.4:-13052047 | None:intergenic |
| CAGGAGCATCATGAGCAGAA+AGG | 0.571515 | 8.4:-13052171 | None:intergenic |
| CATAGATGGTCCACAATGCA+TGG | 0.571731 | 8.4:-13052853 | None:intergenic |
| AAGCCATGTTATTTCAACAC+AGG | 0.579959 | 8.4:+13052541 | MS.gene060376:CDS |
| CTCGCCGTTCCGGAAAGCGG+GGG | 0.586591 | 8.4:-13052014 | None:intergenic |
| GATGACCTATACAAATCGTA+AGG | 0.587720 | 8.4:-13052880 | None:intergenic |
| ACACCTGTGTTGAAATAACA+TGG | 0.589070 | 8.4:-13052544 | None:intergenic |
| TGGAACCAACATGGTTTAGG+TGG | 0.594893 | 8.4:+13052727 | MS.gene060376:CDS |
| CTTTGGGGTGTAGACATGGA+AGG | 0.596287 | 8.4:+13052415 | MS.gene060376:CDS |
| TGGGGTGGAGGAAGGACAGG+AGG | 0.599559 | 8.4:-13051928 | None:intergenic |
| GAGGGAGGTCAGAAGAGGGT+AGG | 0.601209 | 8.4:-13051979 | None:intergenic |
| TTCAAGGAAGCCATGCATTG+TGG | 0.601269 | 8.4:+13052843 | MS.gene060376:CDS |
| CAACACAGGTGTAATGGTGA+TGG | 0.613711 | 8.4:+13052555 | MS.gene060376:CDS |
| TAAGCTTTGGGGTGTAGACA+TGG | 0.613816 | 8.4:+13052411 | MS.gene060376:CDS |
| TAATTACCTTCGTGGAACCA+TGG | 0.617230 | 8.4:+13052090 | MS.gene060376:CDS |
| TTTGGGGTGTAGACATGGAA+GGG | 0.626150 | 8.4:+13052416 | MS.gene060376:CDS |
| AAAAGAGGGAGGTCAGAAGA+GGG | 0.626688 | 8.4:-13051983 | None:intergenic |
| ACGAAGGTAATTAACATCAA+GGG | 0.627230 | 8.4:-13052080 | None:intergenic |
| TATCTCCACCTAAACCATGT+TGG | 0.627381 | 8.4:-13052732 | None:intergenic |
| GATGATATAGCTAAGCTTTG+GGG | 0.638565 | 8.4:+13052400 | MS.gene060376:CDS |
| GATCATAGATGGAACCAACA+TGG | 0.640572 | 8.4:+13052718 | MS.gene060376:CDS |
| GAAAAGGTAAACCATGGTTG+AGG | 0.644192 | 8.4:+13052815 | MS.gene060376:CDS |
| GTAGGCGGACAAGGCCAAGA+CGG | 0.645210 | 8.4:-13051961 | None:intergenic |
| ACATGTTGAATGTTGTAACA+TGG | 0.656912 | 8.4:-13052125 | None:intergenic |
| CCCCGCTTTCCGGAACGGCG+AGG | 0.661029 | 8.4:+13052015 | MS.gene060376:CDS |
| GACGGATAGAGGTAATGGGG+TGG | 0.666414 | 8.4:-13051943 | None:intergenic |
| GCAATAAACTAATTGGACCA+GGG | 0.673769 | 8.4:-13052783 | None:intergenic |
| CAAGACGGATAGAGGTAATG+GGG | 0.681380 | 8.4:-13051946 | None:intergenic |
| CTATATCATCAACCACAACA+AGG | 0.685272 | 8.4:-13052387 | None:intergenic |
| AGACATGGAAGGGAAAGTAG+TGG | 0.686653 | 8.4:+13052426 | MS.gene060376:CDS |
| GGAGTGGAAAAGGTAAACCA+TGG | 0.689517 | 8.4:+13052809 | MS.gene060376:CDS |
| AAATGTAGAAGCTTACACCC+TGG | 0.716978 | 8.4:+13052766 | MS.gene060376:CDS |
| CTGCAGCCATGGTTCCACGA+AGG | 0.735508 | 8.4:-13052096 | None:intergenic |
| CTGTACAAAAGAAATCCAAG+AGG | 0.743316 | 8.4:+13052632 | MS.gene060376:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTATTTCACAAAACAATTT+TGG | + | chr8.4:13052482-13052501 | MS.gene060376:CDS | 20.0% |
| ! | AAATCGTCATGTTTAGATAA+GGG | - | chr8.4:13052225-13052244 | None:intergenic | 25.0% |
| ! | AAATCTGTATGACAATATTC+TGG | - | chr8.4:13052457-13052476 | None:intergenic | 25.0% |
| ! | TAAATCGTCATGTTTAGATA+AGG | - | chr8.4:13052226-13052245 | None:intergenic | 25.0% |
| ! | TATATCCTTGCATAATTCAA+AGG | - | chr8.4:13052313-13052332 | None:intergenic | 25.0% |
| !! | AAAGTCAATTAGAAAAGCAT+TGG | + | chr8.4:13052282-13052301 | MS.gene060376:CDS | 25.0% |
| !!! | TTATTCTTCAAAAAAGTGTC+TGG | - | chr8.4:13052905-13052924 | None:intergenic | 25.0% |
| !!! | TTTCACAAAACAATTTTGGT+CGG | + | chr8.4:13052486-13052505 | MS.gene060376:CDS | 25.0% |
| ACATGTTGAATGTTGTAACA+TGG | - | chr8.4:13052128-13052147 | None:intergenic | 30.0% | |
| ACGAAGGTAATTAACATCAA+GGG | - | chr8.4:13052083-13052102 | None:intergenic | 30.0% | |
| ATACAGAGAAAGTTGAAGAA+TGG | + | chr8.4:13052605-13052624 | MS.gene060376:CDS | 30.0% | |
| ATCAACCTTTGAATTATGCA+AGG | + | chr8.4:13052305-13052324 | MS.gene060376:CDS | 30.0% | |
| ATCCAAGAGGATATATCATT+TGG | + | chr8.4:13052645-13052664 | MS.gene060376:CDS | 30.0% | |
| CTCCAATGCAATAAACTAAT+TGG | - | chr8.4:13052793-13052812 | None:intergenic | 30.0% | |
| TCCAAGAGGATATATCATTT+GGG | + | chr8.4:13052646-13052665 | MS.gene060376:CDS | 30.0% | |
| TGATGATATAGCTAAGCTTT+GGG | + | chr8.4:13052399-13052418 | MS.gene060376:CDS | 30.0% | |
| TTAAAGCAGTTGATCATAGA+TGG | + | chr8.4:13052707-13052726 | MS.gene060376:CDS | 30.0% | |
| TTATTTCAACACAGGTGTAA+TGG | + | chr8.4:13052549-13052568 | MS.gene060376:CDS | 30.0% | |
| TTTATGCTTGTGAATAGTTC+AGG | - | chr8.4:13052193-13052212 | None:intergenic | 30.0% | |
| ! | ATTAGTTTATTGCATTGGAG+TGG | + | chr8.4:13052793-13052812 | MS.gene060376:CDS | 30.0% |
| ! | GTCCAATTAGTTTATTGCAT+TGG | + | chr8.4:13052788-13052807 | MS.gene060376:CDS | 30.0% |
| ! | TTGATGATATAGCTAAGCTT+TGG | + | chr8.4:13052398-13052417 | MS.gene060376:CDS | 30.0% |
| ACACCTGTGTTGAAATAACA+TGG | - | chr8.4:13052547-13052566 | None:intergenic | 35.0% | |
| ACCCAAATGATATATCCTCT+TGG | - | chr8.4:13052650-13052669 | None:intergenic | 35.0% | |
| AGAGAAAGTTGAAGAATGGA+TGG | + | chr8.4:13052609-13052628 | MS.gene060376:CDS | 35.0% | |
| ATTTATACGATCAGCTTCTG+TGG | - | chr8.4:13052050-13052069 | None:intergenic | 35.0% | |
| CACGAAGGTAATTAACATCA+AGG | - | chr8.4:13052084-13052103 | None:intergenic | 35.0% | |
| CTATATCATCAACCACAACA+AGG | - | chr8.4:13052390-13052409 | None:intergenic | 35.0% | |
| CTGTACAAAAGAAATCCAAG+AGG | + | chr8.4:13052632-13052651 | MS.gene060376:CDS | 35.0% | |
| CTTGATGTTAATTACCTTCG+TGG | + | chr8.4:13052082-13052101 | MS.gene060376:CDS | 35.0% | |
| GATGACCTATACAAATCGTA+AGG | - | chr8.4:13052883-13052902 | None:intergenic | 35.0% | |
| GATGATATAGCTAAGCTTTG+GGG | + | chr8.4:13052400-13052419 | MS.gene060376:CDS | 35.0% | |
| GCAATAAACTAATTGGACCA+GGG | - | chr8.4:13052786-13052805 | None:intergenic | 35.0% | |
| GTAATTAACATCAAGGGTCA+TGG | - | chr8.4:13052077-13052096 | None:intergenic | 35.0% | |
| TGCAATAAACTAATTGGACC+AGG | - | chr8.4:13052787-13052806 | None:intergenic | 35.0% | |
| TTAGGTGGAGATAACATTGA+AGG | + | chr8.4:13052742-13052761 | MS.gene060376:CDS | 35.0% | |
| TTATTGCATTGGAGTGGAAA+AGG | + | chr8.4:13052799-13052818 | MS.gene060376:CDS | 35.0% | |
| TTTAATTCTGATCGTGTTCG+CGG | + | chr8.4:13052250-13052269 | MS.gene060376:CDS | 35.0% | |
| TTTAGATTCTGACCTTGTTG+TGG | + | chr8.4:13052375-13052394 | MS.gene060376:CDS | 35.0% | |
| ! | AAGTAGAAGGCAAGATTTTC+TGG | - | chr8.4:13052151-13052170 | None:intergenic | 35.0% |
| ! | ATCACACGTTGTACATTTTC+TGG | - | chr8.4:13052352-13052371 | None:intergenic | 35.0% |
| !! | AAGCCATGTTATTTCAACAC+AGG | + | chr8.4:13052541-13052560 | MS.gene060376:CDS | 35.0% |
| !! | TTCTCTTCCACCATTTTTGT+TGG | + | chr8.4:13052669-13052688 | MS.gene060376:CDS | 35.0% |
| !!! | CATTTTTGTTGGTTTTGGCA+GGG | + | chr8.4:13052680-13052699 | MS.gene060376:CDS | 35.0% |
| !!! | TCCACCATTTTTGTTGGTTT+TGG | + | chr8.4:13052675-13052694 | MS.gene060376:CDS | 35.0% |
| AAATGTAGAAGCTTACACCC+TGG | + | chr8.4:13052766-13052785 | MS.gene060376:CDS | 40.0% | |
| AGATGGAACCAACATGGTTT+AGG | + | chr8.4:13052724-13052743 | MS.gene060376:CDS | 40.0% | |
| ATGAGCAGAAAGGAAGTAGA+AGG | - | chr8.4:13052164-13052183 | None:intergenic | 40.0% | |
| ATGCATTGTGGACCATCTAT+GGG | + | chr8.4:13052855-13052874 | MS.gene060376:CDS | 40.0% | |
| CCTGCCAAAACCAACAAAAA+TGG | - | chr8.4:13052682-13052701 | None:intergenic | 40.0% | |
| CGTCATGTTTAGATAAGGGA+AGG | - | chr8.4:13052221-13052240 | None:intergenic | 40.0% | |
| GAAAAGGTAAACCATGGTTG+AGG | + | chr8.4:13052815-13052834 | MS.gene060376:CDS | 40.0% | |
| GATCATAGATGGAACCAACA+TGG | + | chr8.4:13052718-13052737 | MS.gene060376:CDS | 40.0% | |
| GCCAAAACCAACAAAAATGG+TGG | - | chr8.4:13052679-13052698 | None:intergenic | 40.0% | |
| TAATTACCTTCGTGGAACCA+TGG | + | chr8.4:13052090-13052109 | MS.gene060376:CDS | 40.0% | |
| TATCTCCACCTAAACCATGT+TGG | - | chr8.4:13052735-13052754 | None:intergenic | 40.0% | |
| !! | AAAACAATTTTGGTCGGACC+CGG | + | chr8.4:13052492-13052511 | MS.gene060376:CDS | 40.0% |
| !! | TGGTGATGGATGTGGATAAA+TGG | + | chr8.4:13052569-13052588 | MS.gene060376:CDS | 40.0% |
| !!! | CCATTTTTGTTGGTTTTGGC+AGG | + | chr8.4:13052679-13052698 | MS.gene060376:CDS | 40.0% |
| AAAAAGAGGGAGGTCAGAAG+AGG | - | chr8.4:13051987-13052006 | None:intergenic | 45.0% | |
| AAAAGAGGGAGGTCAGAAGA+GGG | - | chr8.4:13051986-13052005 | None:intergenic | 45.0% | |
| AATCGTAAGGAGCCCATAGA+TGG | - | chr8.4:13052870-13052889 | None:intergenic | 45.0% | |
| AGACATGGAAGGGAAAGTAG+TGG | + | chr8.4:13052426-13052445 | MS.gene060376:CDS | 45.0% | |
| AGGTAAACCATGGTTGAGGT+TGG | + | chr8.4:13052819-13052838 | MS.gene060376:CDS | 45.0% | |
| CAACACAGGTGTAATGGTGA+TGG | + | chr8.4:13052555-13052574 | MS.gene060376:CDS | 45.0% | |
| CAAGACGGATAGAGGTAATG+GGG | - | chr8.4:13051949-13051968 | None:intergenic | 45.0% | |
| CATAGATGGTCCACAATGCA+TGG | - | chr8.4:13052856-13052875 | None:intergenic | 45.0% | |
| CATGCATTGTGGACCATCTA+TGG | + | chr8.4:13052854-13052873 | MS.gene060376:CDS | 45.0% | |
| CATGGTTGAGGTTGGATTCA+AGG | + | chr8.4:13052827-13052846 | MS.gene060376:CDS | 45.0% | |
| CCAAGACGGATAGAGGTAAT+GGG | - | chr8.4:13051950-13051969 | None:intergenic | 45.0% | |
| CGTGTAGTTGTCATACTCCT+TGG | + | chr8.4:13051905-13051924 | MS.gene060376:CDS | 45.0% | |
| CTTGAATCCAACCTCAACCA+TGG | - | chr8.4:13052829-13052848 | None:intergenic | 45.0% | |
| GGAGTGGAAAAGGTAAACCA+TGG | + | chr8.4:13052809-13052828 | MS.gene060376:CDS | 45.0% | |
| GGGCTCCTTACGATTTGTAT+AGG | + | chr8.4:13052875-13052894 | MS.gene060376:CDS | 45.0% | |
| GTTCTCGCAAAGACGTTTGA+TGG | + | chr8.4:13052514-13052533 | MS.gene060376:CDS | 45.0% | |
| TCAAACGTCTTTGCGAGAAC+CGG | - | chr8.4:13052514-13052533 | None:intergenic | 45.0% | |
| TGGAACCAACATGGTTTAGG+TGG | + | chr8.4:13052727-13052746 | MS.gene060376:CDS | 45.0% | |
| TTCAAGGAAGCCATGCATTG+TGG | + | chr8.4:13052843-13052862 | MS.gene060376:CDS | 45.0% | |
| ! | TTTGGGGTGTAGACATGGAA+GGG | + | chr8.4:13052416-13052435 | MS.gene060376:CDS | 45.0% |
| !! | AGGTGTAATGGTGATGGATG+TGG | + | chr8.4:13052561-13052580 | MS.gene060376:CDS | 45.0% |
| !! | TAAGCTTTGGGGTGTAGACA+TGG | + | chr8.4:13052411-13052430 | MS.gene060376:CDS | 45.0% |
| CAAACGTCTTTGCGAGAACC+GGG | - | chr8.4:13052513-13052532 | None:intergenic | 50.0% | |
| CCCATTACCTCTATCCGTCT+TGG | + | chr8.4:13051947-13051966 | MS.gene060376:CDS | 50.0% | |
| GCCAAGACGGATAGAGGTAA+TGG | - | chr8.4:13051951-13051970 | None:intergenic | 50.0% | |
| !! | CAGGAGCATCATGAGCAGAA+AGG | - | chr8.4:13052174-13052193 | None:intergenic | 50.0% |
| !! | CTTTGGGGTGTAGACATGGA+AGG | + | chr8.4:13052415-13052434 | MS.gene060376:CDS | 50.0% |
| CATGGACAACACTGCAGCCA+TGG | - | chr8.4:13052110-13052129 | None:intergenic | 55.0% | |
| CGGGGGCTTCACGAAAAAGA+GGG | - | chr8.4:13052000-13052019 | None:intergenic | 55.0% | |
| GACAAGGCCAAGACGGATAG+AGG | - | chr8.4:13051957-13051976 | None:intergenic | 55.0% | |
| GGGCTTCACGAAAAAGAGGG+AGG | - | chr8.4:13051997-13052016 | None:intergenic | 55.0% | |
| TAATGGGGTGGAGGAAGGAC+AGG | - | chr8.4:13051934-13051953 | None:intergenic | 55.0% | |
| !! | AGAGGTAATGGGGTGGAGGA+AGG | - | chr8.4:13051939-13051958 | None:intergenic | 55.0% |
| !! | GACGGATAGAGGTAATGGGG+TGG | - | chr8.4:13051946-13051965 | None:intergenic | 55.0% |
| !! | GGATAGAGGTAATGGGGTGG+AGG | - | chr8.4:13051943-13051962 | None:intergenic | 55.0% |
| AGCCACATTCCTCGCCGTTC+CGG | - | chr8.4:13052027-13052046 | None:intergenic | 60.0% | |
| CAGAAGAGGGTAGGCGGACA+AGG | - | chr8.4:13051973-13051992 | None:intergenic | 60.0% | |
| CTGCAGCCATGGTTCCACGA+AGG | - | chr8.4:13052099-13052118 | None:intergenic | 60.0% | |
| GAGGGAGGTCAGAAGAGGGT+AGG | - | chr8.4:13051982-13052001 | None:intergenic | 60.0% | |
| GCGGGGGCTTCACGAAAAAG+AGG | - | chr8.4:13052001-13052020 | None:intergenic | 60.0% | |
| GGAGGTCAGAAGAGGGTAGG+CGG | - | chr8.4:13051979-13051998 | None:intergenic | 60.0% | |
| GTAGGCGGACAAGGCCAAGA+CGG | - | chr8.4:13051964-13051983 | None:intergenic | 60.0% | |
| TTCCGGAACGGCGAGGAATG+TGG | + | chr8.4:13052022-13052041 | MS.gene060376:CDS | 60.0% | |
| TTCCTCGCCGTTCCGGAAAG+CGG | - | chr8.4:13052020-13052039 | None:intergenic | 60.0% | |
| TTCGTGAAGCCCCCGCTTTC+CGG | + | chr8.4:13052005-13052024 | MS.gene060376:CDS | 60.0% | |
| GAAGCCCCCGCTTTCCGGAA+CGG | + | chr8.4:13052010-13052029 | MS.gene060376:CDS | 65.0% | |
| GGAGGAAGGACAGGAGGCCA+AGG | - | chr8.4:13051925-13051944 | None:intergenic | 65.0% | |
| TCCTCGCCGTTCCGGAAAGC+GGG | - | chr8.4:13052019-13052038 | None:intergenic | 65.0% | |
| TGGGGTGGAGGAAGGACAGG+AGG | - | chr8.4:13051931-13051950 | None:intergenic | 65.0% | |
| CCTCGCCGTTCCGGAAAGCG+GGG | - | chr8.4:13052018-13052037 | None:intergenic | 70.0% | |
| CTCGCCGTTCCGGAAAGCGG+GGG | - | chr8.4:13052017-13052036 | None:intergenic | 70.0% | |
| CCCCGCTTTCCGGAACGGCG+AGG | + | chr8.4:13052015-13052034 | MS.gene060376:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 13051881 | 13052924 | 13051881 | ID=MS.gene060376 |
| chr8.4 | mRNA | 13051881 | 13052924 | 13051881 | ID=MS.gene060376.t1;Parent=MS.gene060376 |
| chr8.4 | exon | 13051881 | 13052924 | 13051881 | ID=MS.gene060376.t1.exon1;Parent=MS.gene060376.t1 |
| chr8.4 | CDS | 13051881 | 13052924 | 13051881 | ID=cds.MS.gene060376.t1;Parent=MS.gene060376.t1 |
| Gene Sequence |
| Protein sequence |
