Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24583.t1 | XP_003590505.1 | 93.7 | 222 | 14 | 0 | 1 | 222 | 1 | 222 | 4.20E-120 | 440.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24583.t1 | P08688 | 44.1 | 222 | 116 | 6 | 6 | 220 | 7 | 227 | 2.2e-45 | 183.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24583.t1 | G7IBL7 | 93.7 | 222 | 14 | 0 | 1 | 222 | 1 | 222 | 3.0e-120 | 440.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052223 | MS.gene24583 | 0.811978 | 5.34E-51 | -1.69E-46 |
| MS.gene055595 | MS.gene24583 | 0.809693 | 1.67E-50 | -1.69E-46 |
| MS.gene055597 | MS.gene24583 | 0.810291 | 1.24E-50 | -1.69E-46 |
| MS.gene055601 | MS.gene24583 | 0.800124 | 1.67E-48 | -1.69E-46 |
| MS.gene059892 | MS.gene24583 | 0.805838 | 1.10E-49 | -1.69E-46 |
| MS.gene060087 | MS.gene24583 | 0.802118 | 6.53E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24583.t1 | MTR_1g066530 | 93.694 | 222 | 14 | 0 | 1 | 222 | 1 | 222 | 1.24e-158 | 437 |
| MS.gene24583.t1 | MTR_6g073540 | 50.435 | 230 | 105 | 2 | 1 | 221 | 1 | 230 | 8.26e-76 | 228 |
| MS.gene24583.t1 | MTR_6g072470 | 52.000 | 200 | 90 | 1 | 1 | 194 | 1 | 200 | 4.65e-69 | 211 |
| MS.gene24583.t1 | MTR_6g048620 | 49.550 | 222 | 105 | 5 | 6 | 220 | 5 | 226 | 8.24e-67 | 205 |
| MS.gene24583.t1 | MTR_6g045077 | 45.455 | 220 | 115 | 3 | 4 | 220 | 3 | 220 | 6.99e-65 | 200 |
| MS.gene24583.t1 | MTR_6g048910 | 45.909 | 220 | 112 | 4 | 6 | 220 | 5 | 222 | 2.03e-64 | 199 |
| MS.gene24583.t1 | MTR_0695s0030 | 44.091 | 220 | 118 | 4 | 6 | 220 | 65 | 284 | 3.44e-62 | 196 |
| MS.gene24583.t1 | MTR_8g038100 | 44.545 | 220 | 117 | 4 | 6 | 220 | 281 | 500 | 5.95e-60 | 196 |
| MS.gene24583.t1 | MTR_8g038070 | 44.091 | 220 | 118 | 4 | 6 | 220 | 219 | 438 | 6.55e-60 | 195 |
| MS.gene24583.t1 | MTR_0057s0100 | 41.364 | 220 | 124 | 5 | 6 | 220 | 7 | 226 | 4.66e-54 | 173 |
| MS.gene24583.t1 | MTR_0057s0170 | 41.364 | 220 | 124 | 5 | 6 | 220 | 7 | 226 | 2.24e-53 | 171 |
| MS.gene24583.t1 | MTR_7g103990 | 42.152 | 223 | 119 | 8 | 6 | 220 | 7 | 227 | 3.39e-51 | 166 |
| MS.gene24583.t1 | MTR_6g040290 | 39.910 | 223 | 126 | 6 | 6 | 220 | 7 | 229 | 8.62e-46 | 152 |
| MS.gene24583.t1 | MTR_0807s0020 | 37.054 | 224 | 131 | 5 | 1 | 220 | 1 | 218 | 1.10e-45 | 151 |
| MS.gene24583.t1 | MTR_0216s0030 | 38.889 | 198 | 113 | 3 | 4 | 197 | 1 | 194 | 1.05e-44 | 148 |
| MS.gene24583.t1 | MTR_6g048880 | 41.011 | 178 | 95 | 5 | 50 | 220 | 4 | 178 | 1.64e-40 | 137 |
| MS.gene24583.t1 | MTR_7g012000 | 39.423 | 208 | 96 | 5 | 6 | 209 | 7 | 188 | 1.91e-39 | 135 |
| MS.gene24583.t1 | MTR_6g048810 | 51.064 | 141 | 63 | 4 | 84 | 220 | 162 | 300 | 1.03e-37 | 133 |
| MS.gene24583.t1 | MTR_4g102470 | 41.111 | 180 | 83 | 5 | 6 | 182 | 7 | 166 | 2.35e-34 | 122 |
| MS.gene24583.t1 | MTR_6g049040 | 40.876 | 137 | 64 | 3 | 1 | 135 | 1 | 122 | 5.62e-27 | 100 |
| MS.gene24583.t1 | MTR_6g049040 | 34.188 | 117 | 66 | 2 | 72 | 187 | 16 | 122 | 5.87e-16 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCGGTACGGTCTTTGAAAA+TGG | 0.203555 | 1.3:+46765246 | MS.gene24583:CDS |
| ATCCGGAGAGAAATCTATTT+TGG | 0.256716 | 1.3:-46765013 | None:intergenic |
| TTTAACAACACGATCCTTCT+TGG | 0.279901 | 1.3:-46764869 | None:intergenic |
| TAGATCTCTAGAAGTTTAAT+AGG | 0.303436 | 1.3:-46765419 | None:intergenic |
| ACTCATTACTCCATTCGTTC+TGG | 0.346885 | 1.3:+46765210 | MS.gene24583:CDS |
| AACATGGTAGTGATTGGAAC+AGG | 0.421529 | 1.3:-46765060 | None:intergenic |
| AATGGTGTTTCATCAAGAAA+TGG | 0.425643 | 1.3:-46764919 | None:intergenic |
| GTTACGTGTTCAAGAAGAAA+AGG | 0.446425 | 1.3:+46764818 | MS.gene24583:CDS |
| CAATGGAACATGGTAGTGAT+TGG | 0.462327 | 1.3:-46765066 | None:intergenic |
| CAATCTACTTCCTTCTCAAA+TGG | 0.462912 | 1.3:-46764937 | None:intergenic |
| GAATAAGAAAACATTATGTC+CGG | 0.469058 | 1.3:-46765139 | None:intergenic |
| ACCGAAGCAATCATCTTCTC+TGG | 0.471293 | 1.3:+46764979 | MS.gene24583:CDS |
| TGCAGTTGTGAATTATGCTC+CGG | 0.478704 | 1.3:+46764843 | MS.gene24583:CDS |
| TTTAATAGGCTCTGAAGAGC+AGG | 0.498729 | 1.3:-46765405 | None:intergenic |
| GCCAGAGAAGATGATTGCTT+CGG | 0.505795 | 1.3:-46764980 | None:intergenic |
| AACAGGGCCATCGAGTAAAT+CGG | 0.507747 | 1.3:-46765043 | None:intergenic |
| AGTAAATCGGTATCTGAATC+CGG | 0.514277 | 1.3:-46765030 | None:intergenic |
| ACACGATCCTTCTTGGCTCC+CGG | 0.519674 | 1.3:-46764862 | None:intergenic |
| GACAATACAGTCGCCTTCGT+TGG | 0.522082 | 1.3:-46765349 | None:intergenic |
| AAACCATCGGAAGTATGAAT+CGG | 0.529141 | 1.3:-46764895 | None:intergenic |
| TTCGGTGCCTTCTGTCTCAA+AGG | 0.532520 | 1.3:-46764962 | None:intergenic |
| TGTCAATGGCAGAGTAAAGC+TGG | 0.534562 | 1.3:+46765368 | MS.gene24583:CDS |
| AGGAAATGAGTATGTTCGTA+TGG | 0.536361 | 1.3:+46765164 | MS.gene24583:CDS |
| TACCATGTTCCATTGTCTCA+AGG | 0.539896 | 1.3:+46765074 | MS.gene24583:CDS |
| GAAGAGCAGGCCATTCATCA+TGG | 0.540017 | 1.3:-46765392 | None:intergenic |
| TGCCAAAATAGATTTCTCTC+CGG | 0.543347 | 1.3:+46765011 | MS.gene24583:CDS |
| GAATTGACGCTGCAATTAAG+TGG | 0.544957 | 1.3:+46765115 | MS.gene24583:CDS |
| AAGCCGATTCATACTTCCGA+TGG | 0.550807 | 1.3:+46764892 | MS.gene24583:CDS |
| TAAAGCTGGTCCATGATGAA+TGG | 0.552708 | 1.3:+46765382 | MS.gene24583:CDS |
| TTATGCTCCGGGAGCCAAGA+AGG | 0.560419 | 1.3:+46764855 | MS.gene24583:CDS |
| AAAACGTTCCCTTGAGACAA+TGG | 0.561335 | 1.3:-46765083 | None:intergenic |
| TGATGAAACACCATTTGAGA+AGG | 0.562306 | 1.3:+46764927 | MS.gene24583:CDS |
| ATCAAGAAATGGAAAACCAT+CGG | 0.570603 | 1.3:-46764908 | None:intergenic |
| GATTGTGCCTTTGAGACAGA+AGG | 0.572261 | 1.3:+46764955 | MS.gene24583:CDS |
| GCAGTTGTGAATTATGCTCC+GGG | 0.572517 | 1.3:+46764844 | MS.gene24583:CDS |
| ACCATGTTCCATTGTCTCAA+GGG | 0.576700 | 1.3:+46765075 | MS.gene24583:CDS |
| GACGCTGCAATTAAGTGGAC+CGG | 0.578299 | 1.3:+46765120 | MS.gene24583:CDS |
| GAAATGCAGTATCAATGACT+TGG | 0.591249 | 1.3:-46764774 | None:intergenic |
| TCAGATACCGATTTACTCGA+TGG | 0.596226 | 1.3:+46765036 | MS.gene24583:CDS |
| GAAGGCGACTGTATTGTCAA+TGG | 0.599538 | 1.3:+46765354 | MS.gene24583:CDS |
| CGTATTGATATTTCCAACGA+AGG | 0.614900 | 1.3:+46765336 | MS.gene24583:CDS |
| TCCCTTGAGACAATGGAACA+TGG | 0.652701 | 1.3:-46765076 | None:intergenic |
| ACATGGTAGTGATTGGAACA+GGG | 0.671114 | 1.3:-46765059 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATAATGTTTTCTTATTCAA+AGG | + | chr1.3:46765144-46765163 | MS.gene24583:CDS | 20.0% |
| ! | GAATAAGAAAACATTATGTC+CGG | - | chr1.3:46765142-46765161 | None:intergenic | 25.0% |
| AATGGTGTTTCATCAAGAAA+TGG | - | chr1.3:46764922-46764941 | None:intergenic | 30.0% | |
| ATCAAGAAATGGAAAACCAT+CGG | - | chr1.3:46764911-46764930 | None:intergenic | 30.0% | |
| ! | AATCAAGCTTACGTTTTCAA+AGG | + | chr1.3:46765300-46765319 | MS.gene24583:CDS | 30.0% |
| !! | AAGGGAACGTTTTTAGAAAA+TGG | + | chr1.3:46765093-46765112 | MS.gene24583:CDS | 30.0% |
| !! | TTTTAAAAGTTTGCTCGGTA+CGG | + | chr1.3:46765233-46765252 | MS.gene24583:CDS | 30.0% |
| !!! | ACTTTTAAAACCAGAACGAA+TGG | - | chr1.3:46765223-46765242 | None:intergenic | 30.0% |
| !!! | TCTGGTTTTAAAAGTTTGCT+CGG | + | chr1.3:46765228-46765247 | MS.gene24583:CDS | 30.0% |
| AGGAAATGAGTATGTTCGTA+TGG | + | chr1.3:46765164-46765183 | MS.gene24583:CDS | 35.0% | |
| AGTAAATCGGTATCTGAATC+CGG | - | chr1.3:46765033-46765052 | None:intergenic | 35.0% | |
| CAATCTACTTCCTTCTCAAA+TGG | - | chr1.3:46764940-46764959 | None:intergenic | 35.0% | |
| GAAATGCAGTATCAATGACT+TGG | - | chr1.3:46764777-46764796 | None:intergenic | 35.0% | |
| GTTACGTGTTCAAGAAGAAA+AGG | + | chr1.3:46764818-46764837 | MS.gene24583:CDS | 35.0% | |
| TGATGAAACACCATTTGAGA+AGG | + | chr1.3:46764927-46764946 | MS.gene24583:CDS | 35.0% | |
| TGCCAAAATAGATTTCTCTC+CGG | + | chr1.3:46765011-46765030 | MS.gene24583:CDS | 35.0% | |
| TTTAACAACACGATCCTTCT+TGG | - | chr1.3:46764872-46764891 | None:intergenic | 35.0% | |
| ! | AAACCATCGGAAGTATGAAT+CGG | - | chr1.3:46764898-46764917 | None:intergenic | 35.0% |
| ! | ATCCGGAGAGAAATCTATTT+TGG | - | chr1.3:46765016-46765035 | None:intergenic | 35.0% |
| ! | CGTATTGATATTTCCAACGA+AGG | + | chr1.3:46765336-46765355 | MS.gene24583:CDS | 35.0% |
| AAAACGTTCCCTTGAGACAA+TGG | - | chr1.3:46765086-46765105 | None:intergenic | 40.0% | |
| ACCATGTTCCATTGTCTCAA+GGG | + | chr1.3:46765075-46765094 | MS.gene24583:CDS | 40.0% | |
| ACTCATTACTCCATTCGTTC+TGG | + | chr1.3:46765210-46765229 | MS.gene24583:CDS | 40.0% | |
| GAATTGACGCTGCAATTAAG+TGG | + | chr1.3:46765115-46765134 | MS.gene24583:CDS | 40.0% | |
| TAAAGCTGGTCCATGATGAA+TGG | + | chr1.3:46765382-46765401 | MS.gene24583:CDS | 40.0% | |
| TACCATGTTCCATTGTCTCA+AGG | + | chr1.3:46765074-46765093 | MS.gene24583:CDS | 40.0% | |
| TGCAGTTGTGAATTATGCTC+CGG | + | chr1.3:46764843-46764862 | MS.gene24583:CDS | 40.0% | |
| ! | AACATGGTAGTGATTGGAAC+AGG | - | chr1.3:46765063-46765082 | None:intergenic | 40.0% |
| ! | ACATGGTAGTGATTGGAACA+GGG | - | chr1.3:46765062-46765081 | None:intergenic | 40.0% |
| ! | CAATGGAACATGGTAGTGAT+TGG | - | chr1.3:46765069-46765088 | None:intergenic | 40.0% |
| !! | TCAGATACCGATTTACTCGA+TGG | + | chr1.3:46765036-46765055 | MS.gene24583:CDS | 40.0% |
| !! | TTTAATAGGCTCTGAAGAGC+AGG | - | chr1.3:46765408-46765427 | None:intergenic | 40.0% |
| AACAGGGCCATCGAGTAAAT+CGG | - | chr1.3:46765046-46765065 | None:intergenic | 45.0% | |
| ACCGAAGCAATCATCTTCTC+TGG | + | chr1.3:46764979-46764998 | MS.gene24583:CDS | 45.0% | |
| GAAGGCGACTGTATTGTCAA+TGG | + | chr1.3:46765354-46765373 | MS.gene24583:CDS | 45.0% | |
| GCAGTTGTGAATTATGCTCC+GGG | + | chr1.3:46764844-46764863 | MS.gene24583:CDS | 45.0% | |
| GCCAGAGAAGATGATTGCTT+CGG | - | chr1.3:46764983-46765002 | None:intergenic | 45.0% | |
| TCCCTTGAGACAATGGAACA+TGG | - | chr1.3:46765079-46765098 | None:intergenic | 45.0% | |
| TGTCAATGGCAGAGTAAAGC+TGG | + | chr1.3:46765368-46765387 | MS.gene24583:CDS | 45.0% | |
| ! | AAGCCGATTCATACTTCCGA+TGG | + | chr1.3:46764892-46764911 | MS.gene24583:CDS | 45.0% |
| ! | CTCGGTACGGTCTTTGAAAA+TGG | + | chr1.3:46765246-46765265 | MS.gene24583:CDS | 45.0% |
| !! | GATTGTGCCTTTGAGACAGA+AGG | + | chr1.3:46764955-46764974 | MS.gene24583:CDS | 45.0% |
| GAAGAGCAGGCCATTCATCA+TGG | - | chr1.3:46765395-46765414 | None:intergenic | 50.0% | |
| GACAATACAGTCGCCTTCGT+TGG | - | chr1.3:46765352-46765371 | None:intergenic | 50.0% | |
| GACGCTGCAATTAAGTGGAC+CGG | + | chr1.3:46765120-46765139 | MS.gene24583:CDS | 50.0% | |
| !! | TTCGGTGCCTTCTGTCTCAA+AGG | - | chr1.3:46764965-46764984 | None:intergenic | 50.0% |
| ACACGATCCTTCTTGGCTCC+CGG | - | chr1.3:46764865-46764884 | None:intergenic | 55.0% | |
| TTATGCTCCGGGAGCCAAGA+AGG | + | chr1.3:46764855-46764874 | MS.gene24583:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 46764766 | 46765434 | 46764766 | ID=MS.gene24583 |
| chr1.3 | mRNA | 46764766 | 46765434 | 46764766 | ID=MS.gene24583.t1;Parent=MS.gene24583 |
| chr1.3 | exon | 46764766 | 46765434 | 46764766 | ID=MS.gene24583.t1.exon1;Parent=MS.gene24583.t1 |
| chr1.3 | CDS | 46764766 | 46765434 | 46764766 | ID=cds.MS.gene24583.t1;Parent=MS.gene24583.t1 |
| Gene Sequence |
| Protein sequence |
