Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24977.t1 | XP_003604502.1 | 93 | 114 | 8 | 0 | 1 | 114 | 1 | 114 | 7.20E-52 | 213 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24977.t1 | P81748 | 28.2 | 103 | 73 | 1 | 1 | 103 | 1 | 102 | 1.2e-07 | 57.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24977.t1 | G7JJG3 | 93.0 | 114 | 8 | 0 | 1 | 114 | 1 | 114 | 5.2e-52 | 213.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051560 | MS.gene24977 | -0.808944 | 2.41E-50 | -1.69E-46 |
| MS.gene056310 | MS.gene24977 | 0.80615 | 9.46E-50 | -1.69E-46 |
| MS.gene056313 | MS.gene24977 | 0.838548 | 2.69E-57 | -1.69E-46 |
| MS.gene058171 | MS.gene24977 | 0.824727 | 6.90E-54 | -1.69E-46 |
| MS.gene058173 | MS.gene24977 | 0.822897 | 1.85E-53 | -1.69E-46 |
| MS.gene058175 | MS.gene24977 | 0.825773 | 3.90E-54 | -1.69E-46 |
| MS.gene059044 | MS.gene24977 | 0.800209 | 1.60E-48 | -1.69E-46 |
| MS.gene059045 | MS.gene24977 | 0.804775 | 1.84E-49 | -1.69E-46 |
| MS.gene059223 | MS.gene24977 | 0.810455 | 1.14E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24977.t1 | MTR_4g013730 | 92.982 | 114 | 8 | 0 | 1 | 114 | 1 | 114 | 8.35e-75 | 217 |
| MS.gene24977.t1 | MTR_0562s0020 | 92.982 | 114 | 8 | 0 | 1 | 114 | 1 | 114 | 8.35e-75 | 217 |
| MS.gene24977.t1 | MTR_7g070250 | 80.392 | 102 | 20 | 0 | 1 | 102 | 1 | 102 | 8.06e-58 | 174 |
| MS.gene24977.t1 | MTR_4g014040 | 51.327 | 113 | 55 | 0 | 1 | 113 | 1 | 113 | 4.18e-40 | 129 |
| MS.gene24977.t1 | MTR_4g014040 | 56.863 | 102 | 44 | 0 | 1 | 102 | 1 | 102 | 2.73e-39 | 127 |
| MS.gene24977.t1 | MTR_4g014040 | 56.863 | 102 | 44 | 0 | 1 | 102 | 1 | 102 | 6.10e-39 | 128 |
| MS.gene24977.t1 | MTR_7g059460 | 44.860 | 107 | 59 | 0 | 1 | 107 | 1 | 107 | 3.92e-30 | 104 |
| MS.gene24977.t1 | MTR_7g059460 | 44.660 | 103 | 57 | 0 | 1 | 103 | 1 | 103 | 1.55e-29 | 103 |
| MS.gene24977.t1 | MTR_4g127370 | 46.078 | 102 | 55 | 0 | 1 | 102 | 739 | 840 | 9.20e-25 | 97.8 |
| MS.gene24977.t1 | MTR_4g127360 | 41.748 | 103 | 60 | 0 | 1 | 103 | 1 | 103 | 1.30e-23 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24977.t1 | AT3G51660 | 59.223 | 103 | 42 | 0 | 1 | 103 | 1 | 103 | 1.54e-41 | 133 |
| MS.gene24977.t1 | AT5G01650 | 55.882 | 102 | 45 | 0 | 1 | 102 | 1 | 102 | 4.09e-40 | 130 |
| MS.gene24977.t1 | AT5G01650 | 55.882 | 102 | 45 | 0 | 1 | 102 | 1 | 102 | 4.09e-40 | 130 |
| MS.gene24977.t1 | AT5G01650 | 55.882 | 102 | 45 | 0 | 1 | 102 | 1 | 102 | 7.11e-40 | 129 |
| MS.gene24977.t1 | AT5G01650 | 55.882 | 102 | 45 | 0 | 1 | 102 | 1 | 102 | 1.08e-39 | 128 |
| MS.gene24977.t1 | AT5G57170 | 46.078 | 102 | 55 | 0 | 1 | 102 | 1 | 102 | 2.52e-26 | 94.7 |
| MS.gene24977.t1 | AT5G57170 | 46.078 | 102 | 55 | 0 | 1 | 102 | 1 | 102 | 5.43e-26 | 94.0 |
Find 23 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTACCAACGTTAATTTAGA+TGG | 0.290910 | 4.1:-82268066 | MS.gene24977:CDS |
| CAGTACCATTGGCACAATAT+TGG | 0.301904 | 4.1:-82266570 | MS.gene24977:CDS |
| ACTGCTGTTTCCACAATCAT+AGG | 0.405897 | 4.1:-82268009 | MS.gene24977:CDS |
| TCAAAAGCATCACTACAGTT+TGG | 0.409372 | 4.1:+82266469 | None:intergenic |
| TCTCAAAGTCTTTGACATCA+TGG | 0.431478 | 4.1:-82266513 | MS.gene24977:CDS |
| GAAGAGTGACATACCTTGTC+GGG | 0.442658 | 4.1:+82267982 | None:intergenic |
| GTTCTAGCTTGATCAAAGAT+AGG | 0.446427 | 4.1:+82268030 | None:intergenic |
| GTTTGTGATGGTTCTATTGA+AGG | 0.461686 | 4.1:-82266693 | MS.gene24977:CDS |
| TGTTCTTGGTATAGAGAGGT+TGG | 0.461721 | 4.1:+82266544 | None:intergenic |
| AACAAAGAACCAGCTGCATA+TGG | 0.465591 | 4.1:-82266644 | MS.gene24977:CDS |
| TTTGTGATGGTTCTATTGAA+GGG | 0.504158 | 4.1:-82266692 | MS.gene24977:CDS |
| TAACGTTGGTAGAGATGTAA+AGG | 0.513197 | 4.1:+82268076 | None:intergenic |
| ATATGGTGAACTAGTTTCAA+TGG | 0.521191 | 4.1:-82266627 | MS.gene24977:CDS |
| AAGAATCTAATCAGTACCAT+TGG | 0.529298 | 4.1:-82266581 | MS.gene24977:CDS |
| GGATTCCAATATTGTGCCAA+TGG | 0.539625 | 4.1:+82266565 | None:intergenic |
| ATCTTGTTCTTGGTATAGAG+AGG | 0.571975 | 4.1:+82266540 | None:intergenic |
| ACTAGTTCACCATATGCAGC+TGG | 0.580662 | 4.1:+82266635 | None:intergenic |
| TTGTGATGGTTCTATTGAAG+GGG | 0.584227 | 4.1:-82266691 | MS.gene24977:CDS |
| TATGGTGAACTAGTTTCAAT+GGG | 0.596371 | 4.1:-82266626 | MS.gene24977:CDS |
| AGAAGAGTGACATACCTTGT+CGG | 0.618285 | 4.1:+82267981 | None:intergenic |
| GGTGAACTAGTTTCAATGGG+TGG | 0.635436 | 4.1:-82266623 | MS.gene24977:CDS |
| TTGGTAGAGATGTAAAGGCA+AGG | 0.650566 | 4.1:+82268081 | None:intergenic |
| AATCATAGGAAAACCCGACA+AGG | 0.692062 | 4.1:-82267995 | MS.gene24977:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAATAAACTAATTTCAAT+GGG | - | chr4.1:82267098-82267117 | MS.gene24977:intron | 10.0% |
| !! | AAATAAAAAAGAATTTCACT+TGG | + | chr4.1:82267433-82267452 | None:intergenic | 15.0% |
| !! | GTTAAATAAACTAATTTCAA+TGG | - | chr4.1:82267097-82267116 | MS.gene24977:intron | 15.0% |
| !!! | ATTTATTTCTCAAAATCAAA+TGG | - | chr4.1:82266989-82267008 | MS.gene24977:intron | 15.0% |
| !!! | CACAAAATTAATATGAATTT+TGG | - | chr4.1:82266679-82266698 | MS.gene24977:CDS | 15.0% |
| !!! | ATTTTGGATTTTGTTGATTA+TGG | - | chr4.1:82266695-82266714 | MS.gene24977:CDS | 20.0% |
| !!! | TTGTTATTTTCAAACGATAT+TGG | + | chr4.1:82267906-82267925 | None:intergenic | 20.0% |
| ! | AAGTAAGTAAATAGCCAAAT+TGG | - | chr4.1:82267544-82267563 | MS.gene24977:intron | 25.0% |
| ! | ACATACATAGTGATATTTCA+AGG | - | chr4.1:82267693-82267712 | MS.gene24977:intron | 25.0% |
| ! | CCTCTAATGATAAAAATTGA+TGG | + | chr4.1:82267663-82267682 | None:intergenic | 25.0% |
| ! | GCATGGTATAAAAATTTATG+AGG | - | chr4.1:82267755-82267774 | MS.gene24977:intron | 25.0% |
| ! | TTGAGAAAAAATCTTGTTCT+TGG | + | chr4.1:82268038-82268057 | None:intergenic | 25.0% |
| !! | ACTGATTCATAATGTTTGAT+TGG | - | chr4.1:82266764-82266783 | MS.gene24977:intron | 25.0% |
| !! | CCATCAATTTTTATCATTAG+AGG | - | chr4.1:82267660-82267679 | MS.gene24977:intron | 25.0% |
| !! | TGTTTTACAAATAAAGCTTG+TGG | - | chr4.1:82266616-82266635 | MS.gene24977:CDS | 25.0% |
| !! | TTTTCCATCTAAATTAACGT+TGG | + | chr4.1:82266506-82266525 | None:intergenic | 25.0% |
| !!! | ATACCATGCAATTTAGTTTT+GGG | + | chr4.1:82267744-82267763 | None:intergenic | 25.0% |
| !!! | TATACCATGCAATTTAGTTT+TGG | + | chr4.1:82267745-82267764 | None:intergenic | 25.0% |
| AAGAATCTAATCAGTACCAT+TGG | - | chr4.1:82267984-82268003 | MS.gene24977:intron | 30.0% | |
| CAAATAAAGCTTGTGGAATT+TGG | - | chr4.1:82266623-82266642 | MS.gene24977:CDS | 30.0% | |
| GTTGTGTGAAAATCCTTTAA+GGG | - | chr4.1:82266652-82266671 | MS.gene24977:CDS | 30.0% | |
| TAACCCAAAACTAAATTGCA+TGG | - | chr4.1:82267738-82267757 | MS.gene24977:intron | 30.0% | |
| TCTACCAACGTTAATTTAGA+TGG | - | chr4.1:82266499-82266518 | MS.gene24977:CDS | 30.0% | |
| ! | AATTTTGTGTCAACCCTTAA+AGG | + | chr4.1:82266668-82266687 | None:intergenic | 30.0% |
| ! | ATATGGTGAACTAGTTTCAA+TGG | - | chr4.1:82267938-82267957 | MS.gene24977:intron | 30.0% |
| ! | GCTCTTTGATGCAATTTTTA+GGG | - | chr4.1:82267207-82267226 | MS.gene24977:intron | 30.0% |
| ! | GTAATTTTTGCAGTTTGTGA+TGG | - | chr4.1:82267860-82267879 | MS.gene24977:intron | 30.0% |
| ! | TATGGTGAACTAGTTTCAAT+GGG | - | chr4.1:82267939-82267958 | MS.gene24977:intron | 30.0% |
| ! | TGACAAGTTTTCTACAGAAA+AGG | + | chr4.1:82266919-82266938 | None:intergenic | 30.0% |
| ! | TGCTCTTTGATGCAATTTTT+AGG | - | chr4.1:82267206-82267225 | MS.gene24977:intron | 30.0% |
| ! | TTTGTGATGGTTCTATTGAA+GGG | - | chr4.1:82267873-82267892 | MS.gene24977:intron | 30.0% |
| !! | CATAATGTTTGATTGGTTTC+TGG | - | chr4.1:82266771-82266790 | MS.gene24977:intron | 30.0% |
| !! | TGATTGGTTTCTGGTATAAT+TGG | - | chr4.1:82266780-82266799 | MS.gene24977:intron | 30.0% |
| !!! | CAATTTAGTTTTGGGTTATG+TGG | + | chr4.1:82267736-82267755 | None:intergenic | 30.0% |
| AAATAAGACAGCAGACTAGT+TGG | - | chr4.1:82267145-82267164 | MS.gene24977:intron | 35.0% | |
| ACTTGGAACAAAGTACAACA+TGG | + | chr4.1:82267416-82267435 | None:intergenic | 35.0% | |
| AGAAAAGGCAGAGAGTAAAA+GGG | + | chr4.1:82266904-82266923 | None:intergenic | 35.0% | |
| ATCTTGTTCTTGGTATAGAG+AGG | + | chr4.1:82268028-82268047 | None:intergenic | 35.0% | |
| CTTGGAACAAAGTACAACAT+GGG | + | chr4.1:82267415-82267434 | None:intergenic | 35.0% | |
| GGTTGTGTGAAAATCCTTTA+AGG | - | chr4.1:82266651-82266670 | MS.gene24977:CDS | 35.0% | |
| TCTCAAAGTCTTTGACATCA+TGG | - | chr4.1:82268052-82268071 | MS.gene24977:CDS | 35.0% | |
| TCTTTGTGCGCAAAGTTAAT+AGG | + | chr4.1:82267515-82267534 | None:intergenic | 35.0% | |
| ! | AGCTTGTGGAATTTGGTTTA+AGG | - | chr4.1:82266630-82266649 | MS.gene24977:CDS | 35.0% |
| ! | GTTTGTGATGGTTCTATTGA+AGG | - | chr4.1:82267872-82267891 | MS.gene24977:intron | 35.0% |
| ! | TAACGTTGGTAGAGATGTAA+AGG | + | chr4.1:82266492-82266511 | None:intergenic | 35.0% |
| ! | TTGTGATGGTTCTATTGAAG+GGG | - | chr4.1:82267874-82267893 | MS.gene24977:intron | 35.0% |
| ! | TTTGCGCACAAAGAATTTTG+CGG | - | chr4.1:82267521-82267540 | MS.gene24977:intron | 35.0% |
| !! | GTTCTAGCTTGATCAAAGAT+AGG | + | chr4.1:82266538-82266557 | None:intergenic | 35.0% |
| !!! | CACAGTTTTGAGTACCAATT+TGG | + | chr4.1:82267561-82267580 | None:intergenic | 35.0% |
| AACAAAGAACCAGCTGCATA+TGG | - | chr4.1:82267921-82267940 | MS.gene24977:intron | 40.0% | |
| AATCATAGGAAAACCCGACA+AGG | - | chr4.1:82266570-82266589 | MS.gene24977:CDS | 40.0% | |
| AGAAGAGTGACATACCTTGT+CGG | + | chr4.1:82266587-82266606 | None:intergenic | 40.0% | |
| CAGAAAAGGCAGAGAGTAAA+AGG | + | chr4.1:82266905-82266924 | None:intergenic | 40.0% | |
| CAGTACCATTGGCACAATAT+TGG | - | chr4.1:82267995-82268014 | MS.gene24977:intron | 40.0% | |
| CCAGCACATAGAAGTTATCA+TGG | - | chr4.1:82267471-82267490 | MS.gene24977:intron | 40.0% | |
| CCATGATAACTTCTATGTGC+TGG | + | chr4.1:82267474-82267493 | None:intergenic | 40.0% | |
| GATAACTTCTATGTGCTGGA+CGG | + | chr4.1:82267470-82267489 | None:intergenic | 40.0% | |
| GGATTCCAATATTGTGCCAA+TGG | + | chr4.1:82268003-82268022 | None:intergenic | 40.0% | |
| TGTTCTTGGTATAGAGAGGT+TGG | + | chr4.1:82268024-82268043 | None:intergenic | 40.0% | |
| TTGGTAGAGATGTAAAGGCA+AGG | + | chr4.1:82266487-82266506 | None:intergenic | 40.0% | |
| ! | CGATGTGATAGTCTTAGAGT+TGG | - | chr4.1:82267805-82267824 | MS.gene24977:intron | 40.0% |
| !! | ACTGCTGTTTCCACAATCAT+AGG | - | chr4.1:82266556-82266575 | MS.gene24977:CDS | 40.0% |
| ACTAGTTCACCATATGCAGC+TGG | + | chr4.1:82267933-82267952 | None:intergenic | 45.0% | |
| GAAGAGTGACATACCTTGTC+GGG | + | chr4.1:82266586-82266605 | None:intergenic | 45.0% | |
| GAAGTGTACACTTCCCTGTT+TGG | - | chr4.1:82266849-82266868 | MS.gene24977:intron | 45.0% | |
| GCTCAGTGACAAACCAAACA+GGG | + | chr4.1:82266865-82266884 | None:intergenic | 45.0% | |
| GGGTTATGTGGCATTGTTGT+AGG | + | chr4.1:82267724-82267743 | None:intergenic | 45.0% | |
| TGCTCAGTGACAAACCAAAC+AGG | + | chr4.1:82266866-82266885 | None:intergenic | 45.0% | |
| ! | GGTGAACTAGTTTCAATGGG+TGG | - | chr4.1:82267942-82267961 | MS.gene24977:intron | 45.0% |
| ! | GTCGGGTTTTCCTATGATTG+TGG | + | chr4.1:82266569-82266588 | None:intergenic | 45.0% |
| ! | TAAGACAGCAGACTAGTTGG+AGG | - | chr4.1:82267148-82267167 | MS.gene24977:intron | 45.0% |
| ! | TTGGAGGCTTTTCTGTCTGT+AGG | - | chr4.1:82267164-82267183 | MS.gene24977:intron | 45.0% |
| !!! | TTTTTATTGTATAAATATAA+TGG | - | chr4.1:82267072-82267091 | MS.gene24977:intron | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 82266481 | 82268106 | 82266481 | ID=MS.gene24977 |
| chr4.1 | mRNA | 82266481 | 82268106 | 82266481 | ID=MS.gene24977.t1;Parent=MS.gene24977 |
| chr4.1 | exon | 82267996 | 82268106 | 82267996 | ID=MS.gene24977.t1.exon1;Parent=MS.gene24977.t1 |
| chr4.1 | CDS | 82267996 | 82268106 | 82267996 | ID=cds.MS.gene24977.t1;Parent=MS.gene24977.t1 |
| chr4.1 | exon | 82266481 | 82266714 | 82266481 | ID=MS.gene24977.t1.exon2;Parent=MS.gene24977.t1 |
| chr4.1 | CDS | 82266481 | 82266714 | 82266481 | ID=cds.MS.gene24977.t1;Parent=MS.gene24977.t1 |
| Gene Sequence |
| Protein sequence |
