AlfalfaGEDB | MS.gene059044

M. sativa cultivar XinJiangDaYe / MS.gene059044

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004864 GO:0005488 GO:0006950 GO:0006952 GO:0007165 GO:0008150 GO:0008289 GO:0009738 GO:0009755 GO:0009987 GO:0010427 GO:0019208 GO:0019212 GO:0019840 GO:0019888 GO:0030234 GO:0031406 GO:0033293 GO:0036094 GO:0038023 GO:0042562 GO:0043167 GO:0043168 GO:0043177 GO:0043178 GO:0050789 GO:0050794 GO:0050896 GO:0060089 GO:0065007 GO:0098772
PFAM: PF00407.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059044.t1	SNQ45214.1	98.1	158	3	0	1	158	1	158	2.70E-81	311.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059044.t1	P14710	88.0	158	19	0	1	158	1	158	8.5e-76	284.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059044.t1	A0A238U3J0	98.1	158	3	0	1	158	1	158	2.0e-81	311.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050381	MS.gene059044	0.810376	1.19E-50	-1.69E-46
MS.gene055124	MS.gene059044	0.82816	1.05E-54	-1.69E-46
MS.gene056313	MS.gene059044	0.829497	4.98E-55	-1.69E-46
MS.gene058093	MS.gene059044	0.816604	5.08E-52	-1.69E-46
MS.gene058099	MS.gene059044	0.811622	6.38E-51	-1.69E-46
MS.gene058108	MS.gene059044	0.81219	4.80E-51	-1.69E-46
MS.gene058171	MS.gene059044	0.837081	6.41E-57	-1.69E-46
MS.gene058173	MS.gene059044	0.839687	1.37E-57	-1.69E-46
MS.gene058175	MS.gene059044	0.833073	6.57E-56	-1.69E-46
MS.gene058176	MS.gene059044	0.944093	3.67E-103	-1.69E-46
MS.gene058179	MS.gene059044	0.858816	6.35E-63	-1.69E-46
MS.gene058180	MS.gene059044	0.830721	2.50E-55	-1.69E-46
MS.gene059037	MS.gene059044	0.846697	1.85E-59	-1.69E-46
MS.gene059038	MS.gene059044	0.826864	2.15E-54	-1.69E-46
MS.gene059044	MS.gene059045	0.834336	3.18E-56	-1.69E-46
MS.gene059044	MS.gene059223	0.802816	4.69E-49	-1.69E-46
MS.gene059044	MS.gene068296	0.820305	7.36E-53	-1.69E-46
MS.gene059044	MS.gene068297	0.858343	8.79E-63	-1.69E-46
MS.gene059044	MS.gene071838	0.832944	7.08E-56	-1.69E-46
MS.gene059044	MS.gene071842	0.936136	2.81E-97	-1.69E-46
MS.gene059044	MS.gene071843	0.833865	4.17E-56	-1.69E-46
MS.gene059044	MS.gene072806	0.816727	4.76E-52	-1.69E-46
MS.gene059044	MS.gene21757	0.816094	6.60E-52	-1.69E-46
MS.gene059044	MS.gene21758	0.800982	1.12E-48	-1.69E-46
MS.gene059044	MS.gene21867	0.809409	1.92E-50	-1.69E-46
MS.gene059044	MS.gene24974	0.864885	8.81E-65	-1.69E-46
MS.gene059044	MS.gene24977	0.800209	1.60E-48	-1.69E-46
MS.gene059044	MS.gene26403	0.818835	1.59E-52	-1.69E-46
MS.gene059044	MS.gene27550	0.815763	7.83E-52	-1.69E-46
MS.gene059044	MS.gene27779	0.829119	6.15E-55	-1.69E-46
MS.gene059044	MS.gene28724	0.80504	1.62E-49	-1.69E-46
MS.gene059044	MS.gene33428	0.832504	9.10E-56	-1.69E-46
MS.gene059044	MS.gene33429	0.837413	5.28E-57	-1.69E-46
MS.gene059044	MS.gene33682	0.828028	1.13E-54	-1.69E-46
MS.gene059044	MS.gene33683	0.841568	4.39E-58	-1.69E-46
MS.gene059044	MS.gene35105	0.827343	1.65E-54	-1.69E-46
MS.gene059044	MS.gene38097	0.808321	3.28E-50	-1.69E-46
MS.gene059044	MS.gene38155	0.81742	3.33E-52	-1.69E-46
MS.gene059044	MS.gene38173	0.811084	8.35E-51	-1.69E-46
MS.gene059044	MS.gene39533	0.831119	2.00E-55	-1.69E-46
MS.gene059044	MS.gene44720	0.817547	3.12E-52	-1.69E-46
MS.gene059044	MS.gene44722	0.814549	1.46E-51	-1.69E-46
MS.gene059044	MS.gene46024	0.830982	2.16E-55	-1.69E-46
MS.gene059044	MS.gene46025	0.850007	2.24E-60	-1.69E-46
MS.gene059044	MS.gene46027	0.816409	5.61E-52	-1.69E-46
MS.gene059044	MS.gene48921	0.80846	3.06E-50	-1.69E-46
MS.gene059044	MS.gene49886	0.8038	2.93E-49	-1.69E-46
MS.gene059044	MS.gene59001	0.80461	1.99E-49	-1.69E-46
MS.gene059044	MS.gene59005	0.806158	9.42E-50	-1.69E-46
MS.gene059044	MS.gene59394	0.880529	5.09E-70	-1.69E-46
MS.gene059044	MS.gene59395	0.871035	9.28E-67	-1.69E-46
MS.gene059044	MS.gene59396	0.860962	1.43E-63	-1.69E-46
MS.gene059044	MS.gene61194	0.804133	2.50E-49	-1.69E-46
MS.gene059044	MS.gene62120	0.812131	4.95E-51	-1.69E-46
MS.gene059044	MS.gene64686	0.850058	2.17E-60	-1.69E-46
MS.gene059044	MS.gene64687	0.853497	2.30E-61	-1.69E-46
MS.gene059044	MS.gene70420	0.838753	2.38E-57	-1.69E-46
MS.gene059044	MS.gene71525	0.827784	1.29E-54	-1.69E-46
MS.gene059044	MS.gene75392	0.824546	7.61E-54	-1.69E-46
MS.gene059044	MS.gene75396	0.805975	1.03E-49	-1.69E-46
MS.gene059044	MS.gene77471	0.824143	9.47E-54	-1.69E-46
MS.gene059044	MS.gene84568	0.848941	4.45E-60	-1.69E-46
MS.gene059044	MS.gene91973	0.810003	1.43E-50	-1.69E-46
MS.gene059044	MS.gene91976	0.812774	3.58E-51	-1.69E-46
MS.gene059044	MS.gene91977	0.818909	1.53E-52	-1.69E-46
MS.gene059044	MS.gene92808	0.80459	2.01E-49	-1.69E-46
MS.gene059044	MS.gene94790	0.856728	2.64E-62	-1.69E-46
MS.gene059044	MS.gene96173	0.816964	4.21E-52	-1.69E-46
MS.gene059044	MS.gene97499	0.800807	1.21E-48	-1.69E-46
MS.gene059044	MS.gene97500	0.808855	2.52E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059044.t1	MTR_2g035150	96.835	158	5	0	1	158	1	158	8.52e-108	304
MS.gene059044.t1	MTR_2g035130	89.172	157	17	0	1	157	1	157	1.96e-99	283
MS.gene059044.t1	MTR_2g035120	87.342	158	20	0	1	158	1	158	1.78e-97	278
MS.gene059044.t1	MTR_2g035100	85.350	157	23	0	1	157	1	157	2.43e-94	270
MS.gene059044.t1	MTR_2g035105	84.076	157	25	0	1	157	1	157	5.85e-93	266
MS.gene059044.t1	MTR_6g033450	74.684	158	40	0	1	158	1	158	1.19e-82	240
MS.gene059044.t1	MTR_4g120970	74.684	158	40	0	1	158	1	158	1.19e-82	240
MS.gene059044.t1	MTR_2g035320	66.242	157	53	0	1	157	1	157	1.25e-69	207
MS.gene059044.t1	MTR_2g035190	64.331	157	56	0	1	157	1	157	4.83e-67	201
MS.gene059044.t1	MTR_2g035210	63.057	157	58	0	1	157	1	157	1.35e-65	197
MS.gene059044.t1	MTR_2g035220	63.057	157	58	0	1	157	1	157	8.72e-64	192
MS.gene059044.t1	MTR_2g035170	58.599	157	65	0	1	157	1	157	1.31e-61	187
MS.gene059044.t1	MTR_2g035320	60.606	99	39	0	59	157	24	122	7.10e-37	123
MS.gene059044.t1	MTR_2g435310	39.103	156	94	1	1	155	1	156	9.16e-37	124
MS.gene059044.t1	MTR_4g120950	45.062	162	82	5	1	158	1	159	5.44e-36	122
MS.gene059044.t1	MTR_4g120760	39.744	156	93	1	1	155	1	156	1.22e-35	121
MS.gene059044.t1	MTR_4g120760	39.744	156	93	1	1	155	1	156	1.31e-35	121
MS.gene059044.t1	MTR_4g120940	64.045	89	29	1	70	158	35	120	3.34e-32	111

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 37 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAATTTCAACACTCTTGA+TGG	0.245294	2.4:+56016247	None:intergenic
TGATGGCATCAATAACCTTT+GGG	0.301288	2.4:+56016264	None:intergenic
TTGATGGCATCAATAACCTT+TGG	0.345179	2.4:+56016263	None:intergenic
GGCTATAGAGGGTTACTGTT+TGG	0.394448	2.4:-56015739	MS.gene059044:CDS
CTTAGTAGATGATGCTAACT+TGG	0.397103	2.4:-56015958	MS.gene059044:CDS
GCTTACAACTACAGCATTGT+TGG	0.405129	2.4:-56015936	MS.gene059044:CDS
TTTGCAATGGATCCTCCATT+TGG	0.420662	2.4:+56015852	None:intergenic
TATATGCTTTAGTTGTAGTC+AGG	0.421813	2.4:+56015711	None:intergenic
TACAGCATTGTTGGAGGTGT+TGG	0.427537	2.4:-56015927	MS.gene059044:CDS
GAGATCTTCTCTATTGTGTC+TGG	0.431898	2.4:+56015900	None:intergenic
CATCAATAACCTTTGGGGTA+AGG	0.433870	2.4:+56016270	None:intergenic
AAGAGTGTTGAAATTGTTGA+AGG	0.434561	2.4:-56016242	MS.gene059044:CDS
CACTTTGTGCAAGACATGCT+TGG	0.461268	2.4:+56015983	None:intergenic
AGAGCTTTGTAAAGTGTAGC+AGG	0.477836	2.4:+56016311	None:intergenic
AAATTGTCTGCAGGTCCAAA+TGG	0.485800	2.4:-56015867	MS.gene059044:CDS
ACTTTACAAAGCTCTAGTTA+AGG	0.493500	2.4:-56016303	MS.gene059044:CDS
AATAGAGAAGATCTCATTTG+AGG	0.494265	2.4:-56015892	MS.gene059044:CDS
CTCTTGAGTTCCTCTTCACT+AGG	0.501934	2.4:+56015792	None:intergenic
AATGTGAAATACTTCACAAA+AGG	0.503374	2.4:-56015825	MS.gene059044:CDS
TTTCACATTAAGCTTTGCAA+TGG	0.512266	2.4:+56015839	None:intergenic
GTTGAAGGAAACGGTGGTGC+CGG	0.528042	2.4:-56016227	MS.gene059044:CDS
ACTCAAGAGTGGCAAAGCTA+AGG	0.529920	2.4:-56015778	MS.gene059044:CDS
CTCAAGAGTGGCAAAGCTAA+GGG	0.532020	2.4:-56015777	MS.gene059044:CDS
TTTGAGGCTAAATTGTCTGC+AGG	0.535432	2.4:-56015876	MS.gene059044:CDS
GTTGAAATTGTTGAAGGAAA+CGG	0.548798	2.4:-56016236	MS.gene059044:CDS
AGCAGGAGCTACAGTGGAGG+TGG	0.552452	2.4:+56016328	None:intergenic
AAGTGTAGCAGGAGCTACAG+TGG	0.556963	2.4:+56016322	None:intergenic
TACAACTACAGCATTGTTGG+AGG	0.566488	2.4:-56015933	MS.gene059044:CDS
GATGGCATCAATAACCTTTG+GGG	0.578696	2.4:+56016265	None:intergenic
CAAGCATGTCTTGCACAAAG+TGG	0.581918	2.4:-56015982	MS.gene059044:CDS
AGTGGCAAAGCTAAGGGTGA+TGG	0.593000	2.4:-56015771	MS.gene059044:CDS
TTCTGACAACCTTACCCCAA+AGG	0.596498	2.4:-56016279	MS.gene059044:CDS
AGATGTTACTCCTAGTGAAG+AGG	0.628737	2.4:-56015802	MS.gene059044:CDS
TTGTCTGCAGGTCCAAATGG+AGG	0.646240	2.4:-56015864	MS.gene059044:CDS
TGTAGCAGGAGCTACAGTGG+AGG	0.673221	2.4:+56016325	None:intergenic
AGTGAAGAGGAACTCAAGAG+TGG	0.684104	2.4:-56015789	MS.gene059044:CDS
GAAATTGTTGAAGGAAACGG+TGG	0.749751	2.4:-56016233	MS.gene059044:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATGTGAAATACTTCACAAA+AGG	-	chr2.4:56016250-56016269	MS.gene059044:CDS	25.0%
	AAAAAACTCACATTCGTTGA+AGG	-	chr2.4:56015878-56015897	MS.gene059044:CDS	30.0%
	AACAATTTCAACACTCTTGA+TGG	+	chr2.4:56015831-56015850	None:intergenic	30.0%
	AATAGAGAAGATCTCATTTG+AGG	-	chr2.4:56016183-56016202	MS.gene059044:intron	30.0%
	CTTCAAATATGAAGAGATGT+AGG	+	chr2.4:56016019-56016038	None:intergenic	30.0%
	GTTGAAATTGTTGAAGGAAA+CGG	-	chr2.4:56015839-56015858	MS.gene059044:CDS	30.0%
	TGATGTTTGTGATTGTGAAA+CGG	-	chr2.4:56016048-56016067	MS.gene059044:intron	30.0%
	TTTCACATTAAGCTTTGCAA+TGG	+	chr2.4:56016239-56016258	None:intergenic	30.0%
!	ACTTTACAAAGCTCTAGTTA+AGG	-	chr2.4:56015772-56015791	MS.gene059044:CDS	30.0%
!!	AAGAGTGTTGAAATTGTTGA+AGG	-	chr2.4:56015833-56015852	MS.gene059044:CDS	30.0%
!!!	TGTGAGTTTTTTGATAGTTC+CGG	+	chr2.4:56015870-56015889	None:intergenic	30.0%
	CTTAGTAGATGATGCTAACT+TGG	-	chr2.4:56016117-56016136	MS.gene059044:intron	35.0%
	TGATGGCATCAATAACCTTT+GGG	+	chr2.4:56015814-56015833	None:intergenic	35.0%
!	TTGATGGCATCAATAACCTT+TGG	+	chr2.4:56015815-56015834	None:intergenic	35.0%
	AAATTGTCTGCAGGTCCAAA+TGG	-	chr2.4:56016208-56016227	MS.gene059044:CDS	40.0%
	AACTCACATTCGTTGAAGGT+CGG	-	chr2.4:56015882-56015901	MS.gene059044:CDS	40.0%
	AGATGTTACTCCTAGTGAAG+AGG	-	chr2.4:56016273-56016292	MS.gene059044:CDS	40.0%
	GAAATTGTTGAAGGAAACGG+TGG	-	chr2.4:56015842-56015861	MS.gene059044:CDS	40.0%
	GAGATCTTCTCTATTGTGTC+TGG	+	chr2.4:56016178-56016197	None:intergenic	40.0%
	GATGGCATCAATAACCTTTG+GGG	+	chr2.4:56015813-56015832	None:intergenic	40.0%
	GCTTACAACTACAGCATTGT+TGG	-	chr2.4:56016139-56016158	MS.gene059044:intron	40.0%
	TACAACTACAGCATTGTTGG+AGG	-	chr2.4:56016142-56016161	MS.gene059044:intron	40.0%
	TTTGCAATGGATCCTCCATT+TGG	+	chr2.4:56016226-56016245	None:intergenic	40.0%
!	AGAGCTTTGTAAAGTGTAGC+AGG	+	chr2.4:56015767-56015786	None:intergenic	40.0%
!	TTTGAGGCTAAATTGTCTGC+AGG	-	chr2.4:56016199-56016218	MS.gene059044:CDS	40.0%
!!	CATCAATAACCTTTGGGGTA+AGG	+	chr2.4:56015808-56015827	None:intergenic	40.0%
!!	GGTCTTTTCAAGGCTATAGA+GGG	-	chr2.4:56016325-56016344	MS.gene059044:CDS	40.0%
!!	TAAGGGTGATGGTCTTTTCA+AGG	-	chr2.4:56016315-56016334	MS.gene059044:CDS	40.0%
!!	TGGTCTTTTCAAGGCTATAG+AGG	-	chr2.4:56016324-56016343	MS.gene059044:CDS	40.0%
	ACTCAAGAGTGGCAAAGCTA+AGG	-	chr2.4:56016297-56016316	MS.gene059044:CDS	45.0%
	AGTGAAGAGGAACTCAAGAG+TGG	-	chr2.4:56016286-56016305	MS.gene059044:CDS	45.0%
	CAAGCATGTCTTGCACAAAG+TGG	-	chr2.4:56016093-56016112	MS.gene059044:intron	45.0%
	CACTTTGTGCAAGACATGCT+TGG	+	chr2.4:56016095-56016114	None:intergenic	45.0%
	CTCAAGAGTGGCAAAGCTAA+GGG	-	chr2.4:56016298-56016317	MS.gene059044:CDS	45.0%
	CTCTTGAGTTCCTCTTCACT+AGG	+	chr2.4:56016286-56016305	None:intergenic	45.0%
	GAAACGGATCAATGTGCAGA+TGG	-	chr2.4:56016064-56016083	MS.gene059044:intron	45.0%
	GGCTATAGAGGGTTACTGTT+TGG	-	chr2.4:56016336-56016355	MS.gene059044:CDS	45.0%
	TTCTGACAACCTTACCCCAA+AGG	-	chr2.4:56015796-56015815	MS.gene059044:CDS	45.0%
!	TACAGCATTGTTGGAGGTGT+TGG	-	chr2.4:56016148-56016167	MS.gene059044:intron	45.0%
	AAGTGTAGCAGGAGCTACAG+TGG	+	chr2.4:56015756-56015775	None:intergenic	50.0%
	AGTGGCAAAGCTAAGGGTGA+TGG	-	chr2.4:56016304-56016323	MS.gene059044:CDS	50.0%
	TTGTCTGCAGGTCCAAATGG+AGG	-	chr2.4:56016211-56016230	MS.gene059044:CDS	50.0%
	GTTGAAGGAAACGGTGGTGC+CGG	-	chr2.4:56015848-56015867	MS.gene059044:CDS	55.0%
	TGTAGCAGGAGCTACAGTGG+AGG	+	chr2.4:56015753-56015772	None:intergenic	55.0%
	AGCAGGAGCTACAGTGGAGG+TGG	+	chr2.4:56015750-56015769	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	56015719	56016378	56015719	ID=MS.gene059044
chr2.4	mRNA	56015719	56016378	56015719	ID=MS.gene059044.t1;Parent=MS.gene059044
chr2.4	exon	56016198	56016378	56016198	ID=MS.gene059044.t1.exon1;Parent=MS.gene059044.t1
chr2.4	CDS	56016198	56016378	56016198	ID=cds.MS.gene059044.t1;Parent=MS.gene059044.t1
chr2.4	exon	56015719	56016014	56015719	ID=MS.gene059044.t1.exon2;Parent=MS.gene059044.t1
chr2.4	CDS	56015719	56016014	56015719	ID=cds.MS.gene059044.t1;Parent=MS.gene059044.t1

Gene Sequence

>MS.gene059044

ATGGGTGTGTTCAATTTTGAGGATGAAACCACCTCCACTGTAGCTCCTGCTACACTTTACAAAGCTCTAGTTAAGGATTCTGACAACCTTACCCCAAAGGTTATTGATGCCATCAAGAGTGTTGAAATTGTTGAAGGAAACGGTGGTGCCGGAACTATCAAAAAACTCACATTCGTTGAAGGTCGGTATAAATATATTTATGTTATTTTTATAGTCAAATATTAATAATTAATTGTTACGTTAGTGTTTAAAAAAATTATGTTTTCTACATCGAGTCTAAGTTCATCGTCTGAACTACCTACATCTCTTCATATTTGAAGTAATGCTGATGATGTTTGTGATTGTGAAACGGATCAATGTGCAGATGGTGAGACCAAGCATGTCTTGCACAAAGTGGACTTAGTAGATGATGCTAACTTGGCTTACAACTACAGCATTGTTGGAGGTGTTGGTCTTCCAGACACAATAGAGAAGATCTCATTTGAGGCTAAATTGTCTGCAGGTCCAAATGGAGGATCCATTGCAAAGCTTAATGTGAAATACTTCACAAAAGGAGATGTTACTCCTAGTGAAGAGGAACTCAAGAGTGGCAAAGCTAAGGGTGATGGTCTTTTCAAGGCTATAGAGGGTTACTGTTTGGCTAATCCTGACTACAACTAA

Protein sequence

>MS.gene059044.t1

MGVFNFEDETTSTVAPATLYKALVKDSDNLTPKVIDAIKSVEIVEGNGGAGTIKKLTFVEDGETKHVLHKVDLVDDANLAYNYSIVGGVGLPDTIEKISFEAKLSAGPNGGSIAKLNVKYFTKGDVTPSEEELKSGKAKGDGLFKAIEGYCLANPDYN