AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25133


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25133.t1 MTR_8g020750 89.151 212 22 1 1 211 1 212 1.61e-132 371
MS.gene25133.t1 MTR_8g020770 66.981 212 69 1 1 211 1 212 7.38e-101 291
MS.gene25133.t1 MTR_4g010340 64.762 210 68 4 3 211 1 205 9.40e-87 255
MS.gene25133.t1 MTR_8g020760 59.434 212 84 2 1 211 1 211 1.41e-80 239
MS.gene25133.t1 MTR_2g044040 55.450 211 92 2 1 211 1 209 2.91e-78 233
MS.gene25133.t1 MTR_2g019780 59.259 216 79 5 1 211 1 212 3.92e-77 231
MS.gene25133.t1 MTR_8g020630 54.028 211 95 2 1 211 1 209 6.27e-77 230
MS.gene25133.t1 MTR_0284s0010 54.976 211 93 2 1 211 1 209 1.32e-76 229
MS.gene25133.t1 MTR_3g019710 54.502 211 94 2 1 211 1 209 1.35e-76 229
MS.gene25133.t1 MTR_3g073170 57.944 214 83 4 1 211 1 210 9.11e-76 227
MS.gene25133.t1 MTR_2g036120 54.286 210 94 2 1 210 1 208 1.16e-74 224
MS.gene25133.t1 MTR_3g019680 55.122 205 90 2 7 211 6 208 6.82e-74 222
MS.gene25133.t1 MTR_3g019650 54.634 205 91 2 7 211 6 208 1.22e-73 222
MS.gene25133.t1 MTR_8g020650 55.851 188 82 1 24 211 23 209 8.50e-72 217
MS.gene25133.t1 MTR_2g045100 52.683 205 96 1 7 211 6 209 6.91e-71 214
MS.gene25133.t1 MTR_7g011340 56.383 188 81 1 24 211 23 209 8.79e-71 214
MS.gene25133.t1 MTR_8g020763 57.692 182 77 0 30 211 9 190 2.58e-70 213
MS.gene25133.t1 MTR_5g031460 51.185 211 101 2 1 211 1 209 2.04e-69 211
MS.gene25133.t1 MTR_8g020670 50.000 208 103 1 4 211 3 209 1.26e-67 206
MS.gene25133.t1 MTR_2g041550 47.642 212 108 2 1 211 1 210 7.03e-65 199
MS.gene25133.t1 MTR_8g020610 48.544 206 103 2 7 211 4 207 1.46e-62 193
MS.gene25133.t1 MTR_2g041570 44.811 212 114 2 1 211 1 210 3.43e-62 192
MS.gene25133.t1 MTR_8g020590 45.238 210 112 2 3 211 1 208 2.42e-61 190
MS.gene25133.t1 MTR_2g041580 43.868 212 116 2 1 211 1 210 3.17e-61 190
MS.gene25133.t1 MTR_3g019810 43.781 201 106 2 4 204 109 302 3.88e-50 165
MS.gene25133.t1 MTR_6g005330 43.128 211 99 6 3 200 1 203 7.04e-43 144
MS.gene25133.t1 MTR_6g005360 43.814 194 91 5 3 183 1 189 3.15e-41 139
MS.gene25133.t1 MTR_6g005310 44.211 190 88 5 7 183 5 189 3.63e-41 139
MS.gene25133.t1 MTR_6g005340 43.684 190 89 5 7 183 5 189 2.50e-40 137
MS.gene25133.t1 MTR_4g027415 55.372 121 53 1 24 144 23 142 3.65e-40 134
MS.gene25133.t1 MTR_1g079490 39.024 205 110 5 14 207 17 217 1.97e-37 130
MS.gene25133.t1 MTR_1g085140 40.415 193 112 2 15 204 20 212 3.66e-37 129
MS.gene25133.t1 MTR_2g030855 42.487 193 96 4 16 202 19 202 4.43e-36 126
MS.gene25133.t1 MTR_2g031270 43.243 185 90 4 24 202 28 203 1.60e-35 124
MS.gene25133.t1 MTR_1g023770 40.704 199 106 3 7 202 10 199 1.92e-35 124
MS.gene25133.t1 MTR_7g111240 47.260 146 76 1 24 168 25 170 5.03e-35 123
MS.gene25133.t1 MTR_6g005380 43.094 181 90 4 3 172 1 179 6.88e-35 123
MS.gene25133.t1 MTR_1g060120 34.158 202 124 3 11 204 18 218 2.12e-34 122
MS.gene25133.t1 MTR_7g111240 47.260 146 73 2 24 168 25 167 4.03e-34 121
MS.gene25133.t1 MTR_4g017040 38.863 211 115 5 1 202 1 206 7.27e-34 120
MS.gene25133.t1 MTR_5g036570 35.789 190 118 3 24 209 32 221 1.62e-33 120
MS.gene25133.t1 MTR_2g019250 39.336 211 111 6 4 202 1 206 3.67e-33 119
MS.gene25133.t1 MTR_4g017050 38.389 211 113 6 4 202 1 206 3.91e-33 119
MS.gene25133.t1 MTR_5g046410 38.389 211 113 6 4 202 1 206 4.40e-33 119
MS.gene25133.t1 MTR_5g046430 38.389 211 113 6 4 202 1 206 4.40e-33 119
MS.gene25133.t1 MTR_4g017030 38.389 211 113 6 4 202 1 206 4.40e-33 119
MS.gene25133.t1 MTR_1g085500 37.255 204 123 3 6 204 8 211 5.29e-33 118
MS.gene25133.t1 MTR_2g072560 51.754 114 54 1 4 117 3 115 7.01e-32 113
MS.gene25133.t1 MTR_1g112200 33.333 210 130 4 4 210 8 210 5.69e-31 113
MS.gene25133.t1 MTR_2g030895 42.424 165 88 3 42 202 7 168 4.08e-30 110
MS.gene25133.t1 MTR_6g005350 38.500 200 105 5 3 189 1 195 2.86e-29 108
MS.gene25133.t1 MTR_2g030865 42.424 165 88 3 42 202 7 168 1.35e-28 105
MS.gene25133.t1 MTR_8g007300 44.030 134 73 2 38 170 47 179 1.65e-28 107
MS.gene25133.t1 MTR_3g019610 48.515 101 51 1 7 107 6 105 9.92e-27 99.0
MS.gene25133.t1 MTR_2g031300 48.113 106 52 1 42 144 7 112 1.30e-23 92.4
MS.gene25133.t1 MTR_3g096330 32.227 211 130 4 8 206 560 769 7.89e-23 96.3
MS.gene25133.t1 MTR_2g086620 38.168 131 79 2 79 207 74 204 9.69e-20 83.6
MS.gene25133.t1 MTR_2g086630 38.168 131 79 2 79 207 74 204 1.30e-19 83.2
MS.gene25133.t1 MTR_2g096110 36.184 152 70 5 49 196 10 138 6.86e-19 79.7
MS.gene25133.t1 MTR_4g052770 37.313 134 82 2 76 207 72 205 1.20e-18 80.9
MS.gene25133.t1 MTR_2g086640 36.842 133 80 4 78 207 72 203 1.95e-18 80.1
MS.gene25133.t1 MTR_4g052780 37.313 134 82 2 76 207 72 205 2.20e-18 80.1
MS.gene25133.t1 MTR_7g029500 66.038 53 18 0 152 204 38 90 4.49e-15 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25133.t1 AT5G20630 46.445 211 111 2 1 210 1 210 1.03e-59 186
MS.gene25133.t1 AT1G72610 47.090 189 99 1 24 211 20 208 3.33e-57 180
MS.gene25133.t1 AT5G38960 45.366 205 93 6 20 208 22 223 3.33e-46 152
MS.gene25133.t1 AT5G38940 42.500 200 102 4 18 207 25 221 6.17e-42 141
MS.gene25133.t1 AT5G38940 42.500 200 102 4 18 207 49 245 1.21e-41 141
MS.gene25133.t1 AT5G39160 40.526 190 104 3 25 208 30 216 2.33e-41 140
MS.gene25133.t1 AT5G39110 45.509 167 85 2 25 185 30 196 2.41e-40 137
MS.gene25133.t1 AT3G04200 42.051 195 100 4 24 208 32 223 2.88e-40 137
MS.gene25133.t1 AT5G38930 40.394 203 108 4 15 207 22 221 6.52e-40 136
MS.gene25133.t1 AT5G39190 40.314 191 107 3 25 208 30 220 8.87e-40 136
MS.gene25133.t1 AT5G39120 41.624 197 99 4 1 185 3 195 1.03e-39 135
MS.gene25133.t1 AT5G39160 40.314 191 107 3 25 208 30 220 1.09e-39 135
MS.gene25133.t1 AT5G39180 41.026 195 101 3 1 185 5 195 1.92e-39 135
MS.gene25133.t1 AT5G26700 40.394 203 115 3 3 204 9 206 3.11e-39 134
MS.gene25133.t1 AT3G05950 46.061 165 78 3 25 181 32 193 4.94e-39 134
MS.gene25133.t1 AT5G39150 41.026 195 101 3 1 185 5 195 6.76e-39 134
MS.gene25133.t1 AT4G14630 41.538 195 98 4 25 207 32 222 1.20e-38 133
MS.gene25133.t1 AT5G39130 39.791 191 108 3 25 208 30 220 1.81e-38 132
MS.gene25133.t1 AT5G38910 42.029 207 101 7 14 207 19 219 1.85e-38 132
MS.gene25133.t1 AT1G18980 38.537 205 121 3 6 207 16 218 1.59e-37 130
MS.gene25133.t1 AT3G05930 44.149 188 101 3 24 207 28 215 3.56e-37 129
MS.gene25133.t1 AT1G09560 43.678 174 94 2 16 185 18 191 7.46e-36 125
MS.gene25133.t1 AT1G18970 37.915 211 123 4 3 207 10 218 8.81e-36 125
MS.gene25133.t1 AT1G02335 37.838 185 112 2 24 205 29 213 3.32e-35 124
MS.gene25133.t1 AT3G04190 39.267 191 109 3 24 207 31 221 5.52e-35 124
MS.gene25133.t1 AT5G39190 37.037 189 113 2 26 208 10 198 7.30e-35 122
MS.gene25133.t1 AT5G39160 37.037 189 113 2 26 208 10 198 8.86e-35 122
MS.gene25133.t1 AT3G04150 36.410 195 117 3 24 211 31 225 3.75e-34 122
MS.gene25133.t1 AT5G61750 44.118 136 75 1 38 172 40 175 6.73e-34 120
MS.gene25133.t1 AT3G62020 36.321 212 126 4 5 207 4 215 1.46e-33 120
MS.gene25133.t1 AT3G04150 36.932 176 105 2 42 211 63 238 1.49e-33 120
MS.gene25133.t1 AT3G04180 37.895 190 111 3 25 207 32 221 1.60e-31 114
MS.gene25133.t1 AT3G62020 37.931 174 104 3 38 207 13 186 4.09e-30 110
MS.gene25133.t1 AT3G04170 38.372 172 97 4 42 207 50 218 4.58e-29 108
MS.gene25133.t1 AT1G10460 35.928 167 96 4 21 176 16 182 8.55e-28 105
MS.gene25133.t1 AT1G74820 39.877 163 85 4 4 155 11 171 1.72e-27 104
MS.gene25133.t1 AT3G10080 33.854 192 116 4 24 207 31 219 2.29e-27 104
MS.gene25133.t1 AT3G10080 34.375 192 115 4 24 207 102 290 1.18e-26 103
MS.gene25133.t1 AT5G38910 33.824 204 85 7 14 207 19 182 6.76e-21 86.3

Find 34 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAACTAGTGCTTTGTTATT+TGG 0.151155 8.4:+72853963 MS.gene25133:CDS
TGAAAATGTAATAGTCCTTT+TGG 0.222682 8.4:-72853873 None:intergenic
GAGGTTAATCCAACTTTAAA+AGG 0.227849 8.4:-72853642 None:intergenic
AGAATAGATATTGGTATAAA+TGG 0.231195 8.4:+72853717 MS.gene25133:CDS
CTCTTAACCCTGGTGATGTT+TGG 0.270560 8.4:+72853844 MS.gene25133:CDS
TATCACACCTACAAAACTTT+TGG 0.293680 8.4:+72853815 MS.gene25133:CDS
TCTTAACCCTGGTGATGTTT+GGG 0.343326 8.4:+72853845 MS.gene25133:CDS
TTGCTTTCAGACACCCCTTC+AGG 0.354847 8.4:+72853537 MS.gene25133:CDS
CATTGGAGGTAAATGAGATT+AGG 0.367587 8.4:-72853479 None:intergenic
ATTGGAGGTAAATGAGATTA+GGG 0.404625 8.4:-72853478 None:intergenic
TGCACAAATTATGAAGCTTA+AGG 0.415304 8.4:+72854034 MS.gene25133:CDS
ATTAGTAATTCAGTTGCATC+AGG 0.442131 8.4:-72853762 None:intergenic
TACTCTAGCTTAGCAGCTGC+TGG 0.445392 8.4:+72853606 MS.gene25133:CDS
TTTCAAGTTAACTCTGGTGC+TGG 0.446623 8.4:+72853891 MS.gene25133:CDS
GAATTACTAATCGTTGTTCA+AGG 0.451387 8.4:+72853774 MS.gene25133:CDS
AAGGATGTGTTTGGTGGCAG+TGG 0.462003 8.4:+72854053 MS.gene25133:CDS
ATGAAGCTTAAGGATGTGTT+TGG 0.464532 8.4:+72854044 MS.gene25133:CDS
TCTGCAGCAAGAATAGATAT+TGG 0.476981 8.4:+72853708 MS.gene25133:CDS
GCACTAGTTACATGTATGCT+AGG 0.496669 8.4:-72853951 None:intergenic
AGGTTAATCCAACTTTAAAA+GGG 0.506034 8.4:-72853641 None:intergenic
AGTAATTTACCAGGTCTCAA+TGG 0.507923 8.4:+72853675 MS.gene25133:CDS
ATCATCAGACTCAATAGCAT+TGG 0.511181 8.4:-72853496 None:intergenic
ATCAGACTCAATAGCATTGG+AGG 0.512491 8.4:-72853493 None:intergenic
TGTGTTTGGTGGCAGTGGCT+AGG 0.536076 8.4:+72854058 MS.gene25133:CDS
CACTAGTTACATGTATGCTA+GGG 0.551616 8.4:-72853950 None:intergenic
GATTTGCATGGATAACCTGA+AGG 0.562918 8.4:-72853552 None:intergenic
AAGCTTAAGGATGTGTTTGG+TGG 0.598066 8.4:+72854047 MS.gene25133:CDS
TTGGTGAAAACTCTTAACCC+TGG 0.612922 8.4:+72853834 MS.gene25133:CDS
GTAAATGTCAGTAATTTACC+AGG 0.629129 8.4:+72853666 MS.gene25133:CDS
AACTTAAGTCCATTGAGACC+TGG 0.634682 8.4:-72853684 None:intergenic
TAAATTACTGACATTTACTG+AGG 0.644593 8.4:-72853661 None:intergenic
GCATCAGGATGAGTGTGCAT+TGG 0.654477 8.4:-72853747 None:intergenic
ATTTGCATGGATAACCTGAA+GGG 0.657926 8.4:-72853551 None:intergenic
TTTGCATGGATAACCTGAAG+GGG 0.726823 8.4:-72853550 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATAGATATTGGTATAAA+TGG + chr8.4:72853717-72853736 MS.gene25133:CDS 20.0%
!! CAAAAAAGAAAAGAACAATT+CGG - chr8.4:72853458-72853477 None:intergenic 20.0%
! TAAATTACTGACATTTACTG+AGG - chr8.4:72853664-72853683 None:intergenic 25.0%
!! AGGTTAATCCAACTTTAAAA+GGG - chr8.4:72853644-72853663 None:intergenic 25.0%
!! TGAAAATGTAATAGTCCTTT+TGG - chr8.4:72853876-72853895 None:intergenic 25.0%
!!! ACAAATTTTTCTGATTTGCA+TGG - chr8.4:72853567-72853586 None:intergenic 25.0%
!!! TTACATTTTCAAGTTAACTC+TGG + chr8.4:72853885-72853904 MS.gene25133:CDS 25.0%
ATTAGTAATTCAGTTGCATC+AGG - chr8.4:72853765-72853784 None:intergenic 30.0%
ATTGGAGGTAAATGAGATTA+GGG - chr8.4:72853481-72853500 None:intergenic 30.0%
GAATTACTAATCGTTGTTCA+AGG + chr8.4:72853774-72853793 MS.gene25133:CDS 30.0%
GAGGTTAATCCAACTTTAAA+AGG - chr8.4:72853645-72853664 None:intergenic 30.0%
GTAAATGTCAGTAATTTACC+AGG + chr8.4:72853666-72853685 MS.gene25133:CDS 30.0%
TGCACAAATTATGAAGCTTA+AGG + chr8.4:72854034-72854053 MS.gene25133:CDS 30.0%
! AAGAGTAGTCTTTTGAATTG+TGG - chr8.4:72854006-72854025 None:intergenic 30.0%
! TATCACACCTACAAAACTTT+TGG + chr8.4:72853815-72853834 MS.gene25133:CDS 30.0%
!! GTAACTAGTGCTTTGTTATT+TGG + chr8.4:72853963-72853982 MS.gene25133:CDS 30.0%
!! GTTTTCACCAAAAGTTTTGT+AGG - chr8.4:72853825-72853844 None:intergenic 30.0%
!!! GTTCAAGGTCAATTTTTTGT+TGG + chr8.4:72853789-72853808 MS.gene25133:CDS 30.0%
AGTAATTTACCAGGTCTCAA+TGG + chr8.4:72853675-72853694 MS.gene25133:CDS 35.0%
ATCATCAGACTCAATAGCAT+TGG - chr8.4:72853499-72853518 None:intergenic 35.0%
ATGAAGCTTAAGGATGTGTT+TGG + chr8.4:72854044-72854063 MS.gene25133:CDS 35.0%
ATTTGCATGGATAACCTGAA+GGG - chr8.4:72853554-72853573 None:intergenic 35.0%
CACTAGTTACATGTATGCTA+GGG - chr8.4:72853953-72853972 None:intergenic 35.0%
CATTGGAGGTAAATGAGATT+AGG - chr8.4:72853482-72853501 None:intergenic 35.0%
TCTGCAGCAAGAATAGATAT+TGG + chr8.4:72853708-72853727 MS.gene25133:CDS 35.0%
! GTCTTTTGAATTGTGGAAGT+TGG - chr8.4:72853999-72854018 None:intergenic 35.0%
!! GATGTTTGGGTTTTTCCAAA+AGG + chr8.4:72853858-72853877 MS.gene25133:CDS 35.0%
!!! ACAGCAAACCCTTTTAAAGT+TGG + chr8.4:72853633-72853652 MS.gene25133:CDS 35.0%
AACTTAAGTCCATTGAGACC+TGG - chr8.4:72853687-72853706 None:intergenic 40.0%
AAGCTTAAGGATGTGTTTGG+TGG + chr8.4:72854047-72854066 MS.gene25133:CDS 40.0%
GAAAAACCCAAACATCACCA+GGG - chr8.4:72853854-72853873 None:intergenic 40.0%
GATTTGCATGGATAACCTGA+AGG - chr8.4:72853555-72853574 None:intergenic 40.0%
GCACTAGTTACATGTATGCT+AGG - chr8.4:72853954-72853973 None:intergenic 40.0%
TTGGTGAAAACTCTTAACCC+TGG + chr8.4:72853834-72853853 MS.gene25133:CDS 40.0%
TTTGCATGGATAACCTGAAG+GGG - chr8.4:72853553-72853572 None:intergenic 40.0%
! ATCAGACTCAATAGCATTGG+AGG - chr8.4:72853496-72853515 None:intergenic 40.0%
! TCTTAACCCTGGTGATGTTT+GGG + chr8.4:72853845-72853864 MS.gene25133:CDS 40.0%
!! TTTCAAGTTAACTCTGGTGC+TGG + chr8.4:72853891-72853910 MS.gene25133:CDS 40.0%
GGAAAAACCCAAACATCACC+AGG - chr8.4:72853855-72853874 None:intergenic 45.0%
! CTCTTAACCCTGGTGATGTT+TGG + chr8.4:72853844-72853863 MS.gene25133:CDS 45.0%
GCATCAGGATGAGTGTGCAT+TGG - chr8.4:72853750-72853769 None:intergenic 50.0%
TTGCTTTCAGACACCCCTTC+AGG + chr8.4:72853537-72853556 MS.gene25133:CDS 50.0%
! AAGGATGTGTTTGGTGGCAG+TGG + chr8.4:72854053-72854072 MS.gene25133:CDS 50.0%
! TACTCTAGCTTAGCAGCTGC+TGG + chr8.4:72853606-72853625 MS.gene25133:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 72853444 72854079 72853444 ID=MS.gene25133
chr8.4 mRNA 72853444 72854079 72853444 ID=MS.gene25133.t1;Parent=MS.gene25133
chr8.4 exon 72853444 72854079 72853444 ID=MS.gene25133.t1.exon1;Parent=MS.gene25133.t1
chr8.4 CDS 72853444 72854079 72853444 ID=cds.MS.gene25133.t1;Parent=MS.gene25133.t1
Gene Sequence

>MS.gene25133

ATGAATATGATCCGAATTGTTCTTTTCTTTTTTGCCCTAATCTCATTTACCTCCAATGCTATTGAGTCTGATGATTTCTGTGTAGCAAACTTATTGCTTTCAGACACCCCTTCAGGTTATCCATGCAAATCAGAAAAATTTGTAACAGCGAATGATTTCGCGTACTCTAGCTTAGCAGCTGCTGGAAGCACAGCAAACCCTTTTAAAGTTGGATTAACCTCAGTAAATGTCAGTAATTTACCAGGTCTCAATGGACTTAAGTTATCTGCAGCAAGAATAGATATTGGTATAAATGGAAGTGTACCAATGCACACTCATCCTGATGCAACTGAATTACTAATCGTTGTTCAAGGTCAATTTTTTGTTGGATTTATCACACCTACAAAACTTTTGGTGAAAACTCTTAACCCTGGTGATGTTTGGGTTTTTCCAAAAGGACTATTACATTTTCAAGTTAACTCTGGTGCTGGAAATGCTATTGCTTATGCTGCTTTTAGTAGCTCAAACCCTAGCATACATGTAACTAGTGCTTTGTTATTTGGTAACAACTTACCAACTTCCACAATTCAAAAGACTACTCTTCTTGATGCTGCACAAATTATGAAGCTTAAGGATGTGTTTGGTGGCAGTGGCTAG

Protein sequence

>MS.gene25133.t1

MNMIRIVLFFFALISFTSNAIESDDFCVANLLLSDTPSGYPCKSEKFVTANDFAYSSLAAAGSTANPFKVGLTSVNVSNLPGLNGLKLSAARIDIGINGSVPMHTHPDATELLIVVQGQFFVGFITPTKLLVKTLNPGDVWVFPKGLLHFQVNSGAGNAIAYAAFSSSNPSIHVTSALLFGNNLPTSTIQKTTLLDAAQIMKLKDVFGGSG