AlfalfaGEDB | MS.gene25238

M. sativa cultivar XinJiangDaYe / MS.gene25238

KEGG: -
GO: GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:1901564 GO:1901566 GO:1901576
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25238.t1	XP_004139812.1	100	85	0	0	1	85	123	207	2.40E-36	161

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25238.t1	Q9ZUT9	98.8	85	1	0	1	85	123	207	1.7e-38	159.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene25238.t1	A0A1S3UYH4	100.0	85	0	0	1	85	123	207	1.8e-36	161.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050134	MS.gene25238	0.813976	1.95E-51	-1.69E-46
MS.gene050791	MS.gene25238	0.827353	1.64E-54	-1.69E-46
MS.gene052664	MS.gene25238	0.817492	3.21E-52	-1.69E-46
MS.gene053224	MS.gene25238	0.836938	6.98E-57	-1.69E-46
MS.gene057990	MS.gene25238	0.809231	2.10E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGCTATGCGATCAAGAAAA+AGG	0.316264	7.1:+5914375	MS.gene25238:intron
TCCTCTTCGTCGGGTTAATC+AGG	0.327028	7.1:+5914139	MS.gene25238:CDS
ACCAGTTGTTAGAAGATATA+TGG	0.352278	7.1:-5914162	None:intergenic
AGAGAAGATGCAACCAGAAT+TGG	0.353281	7.1:+5914077	MS.gene25238:CDS
GTGCCCGTGAAGCTGCATTT+AGG	0.361118	7.1:+5914183	MS.gene25238:CDS
TTGGATCTGCTGGAGTTGTT+AGG	0.369348	7.1:+5914096	MS.gene25238:CDS
GCCATATATCTTCTAACAAC+TGG	0.404354	7.1:+5914161	MS.gene25238:CDS
GCAACCAGAATTGGATCTGC+TGG	0.442717	7.1:+5914086	MS.gene25238:CDS
TGGATATCTCTCCTCTTCGT+CGG	0.449849	7.1:+5914129	MS.gene25238:CDS
TGCTGGAGTTGTTAGGAGGC+AGG	0.469034	7.1:+5914103	MS.gene25238:CDS
TGATGCTTAACGATTGGCCT+TGG	0.471931	7.1:-5914416	None:intergenic
CTGCCAAGGGTTCATCCAAC+AGG	0.475684	7.1:+5914252	MS.gene25238:CDS
ATTCTGGTTGCATCTTCTCT+AGG	0.476377	7.1:-5914074	None:intergenic
TGTTCCTAAATGCAGCTTCA+CGG	0.488373	7.1:-5914187	None:intergenic
AACTCCAGCAGATCCAATTC+TGG	0.495047	7.1:-5914090	None:intergenic
TCCAAATGATGCTTAACGAT+TGG	0.503018	7.1:-5914422	None:intergenic
TAAGACAATTGCTGAATGCT+TGG	0.507627	7.1:+5914211	MS.gene25238:CDS
GGATATCTCTCCTCTTCGTC+GGG	0.512011	7.1:+5914130	MS.gene25238:CDS
GTTCCTAAATGCAGCTTCAC+GGG	0.512374	7.1:-5914186	None:intergenic
AGTTGTTAGGAGGCAGGCTG+TGG	0.516790	7.1:+5914109	MS.gene25238:CDS
TGAGATTGAGAGAGTTGCCA+AGG	0.553654	7.1:+5914399	MS.gene25238:CDS
ATACCTGTTGGATGAACCCT+TGG	0.559348	7.1:-5914255	None:intergenic
GATCTGCTGGAGTTGTTAGG+AGG	0.561323	7.1:+5914099	MS.gene25238:CDS
TGAACTCATTAACGCTGCCA+AGG	0.562075	7.1:+5914238	MS.gene25238:CDS
GAACTCATTAACGCTGCCAA+GGG	0.615553	7.1:+5914239	MS.gene25238:CDS
GCCTGATTAACCCGACGAAG+AGG	0.622409	7.1:-5914140	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAACTTTAAAGATTGAATAA+TGG	-	chr7.1:5914285-5914304	None:intergenic	15.0%
!	AGAAAACAAATTCAGTACAT+TGG	-	chr7.1:5914308-5914327	None:intergenic	25.0%
!!	CTTTAAAGATTGAATAATGG+TGG	-	chr7.1:5914282-5914301	None:intergenic	25.0%
	ACCAGTTGTTAGAAGATATA+TGG	-	chr7.1:5914165-5914184	None:intergenic	30.0%
!!	GTACTGAATTTGTTTTCTCT+TGG	+	chr7.1:5914310-5914329	MS.gene25238:intron	30.0%
	AATAATGGTGGTATACCTGT+TGG	-	chr7.1:5914270-5914289	None:intergenic	35.0%
	GCCATATATCTTCTAACAAC+TGG	+	chr7.1:5914161-5914180	MS.gene25238:CDS	35.0%
	TAAGACAATTGCTGAATGCT+TGG	+	chr7.1:5914211-5914230	MS.gene25238:CDS	35.0%
	TGTTAGCAAAGAACCAAACA+AGG	-	chr7.1:5914342-5914361	None:intergenic	35.0%
!	CTCTTGGATTTTTCCTTGTT+TGG	+	chr7.1:5914326-5914345	MS.gene25238:intron	35.0%
	AGAGAAGATGCAACCAGAAT+TGG	+	chr7.1:5914077-5914096	MS.gene25238:CDS	40.0%
	CAGCTATGCGATCAAGAAAA+AGG	+	chr7.1:5914375-5914394	MS.gene25238:intron	40.0%
	TGTTCCTAAATGCAGCTTCA+CGG	-	chr7.1:5914190-5914209	None:intergenic	40.0%
!	ATTCTGGTTGCATCTTCTCT+AGG	-	chr7.1:5914077-5914096	None:intergenic	40.0%
	AACTCCAGCAGATCCAATTC+TGG	-	chr7.1:5914093-5914112	None:intergenic	45.0%
	ATACCTGTTGGATGAACCCT+TGG	-	chr7.1:5914258-5914277	None:intergenic	45.0%
	GAACTCATTAACGCTGCCAA+GGG	+	chr7.1:5914239-5914258	MS.gene25238:CDS	45.0%
	GTTCCTAAATGCAGCTTCAC+GGG	-	chr7.1:5914189-5914208	None:intergenic	45.0%
	TGAACTCATTAACGCTGCCA+AGG	+	chr7.1:5914238-5914257	MS.gene25238:CDS	45.0%
	TGAGATTGAGAGAGTTGCCA+AGG	+	chr7.1:5914399-5914418	MS.gene25238:CDS	45.0%
	TGGATATCTCTCCTCTTCGT+CGG	+	chr7.1:5914129-5914148	MS.gene25238:CDS	45.0%
	TTGCCAAGGCCAATCGTTAA+TGG	+	chr7.1:5914413-5914432	MS.gene25238:CDS	45.0%
	TTGGATCTGCTGGAGTTGTT+AGG	+	chr7.1:5914096-5914115	MS.gene25238:CDS	45.0%
	GATCTGCTGGAGTTGTTAGG+AGG	+	chr7.1:5914099-5914118	MS.gene25238:CDS	50.0%
	GCAACCAGAATTGGATCTGC+TGG	+	chr7.1:5914086-5914105	MS.gene25238:CDS	50.0%
	GGATATCTCTCCTCTTCGTC+GGG	+	chr7.1:5914130-5914149	MS.gene25238:CDS	50.0%
	TCCTCTTCGTCGGGTTAATC+AGG	+	chr7.1:5914139-5914158	MS.gene25238:CDS	50.0%
	AGTTGTTAGGAGGCAGGCTG+TGG	+	chr7.1:5914109-5914128	MS.gene25238:CDS	55.0%
	CTGCCAAGGGTTCATCCAAC+AGG	+	chr7.1:5914252-5914271	MS.gene25238:CDS	55.0%
	GCCTGATTAACCCGACGAAG+AGG	-	chr7.1:5914143-5914162	None:intergenic	55.0%
	GTGCCCGTGAAGCTGCATTT+AGG	+	chr7.1:5914183-5914202	MS.gene25238:CDS	55.0%
	TGCTGGAGTTGTTAGGAGGC+AGG	+	chr7.1:5914103-5914122	MS.gene25238:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	5914070	5914432	5914070	ID=MS.gene25238
chr7.1	mRNA	5914070	5914432	5914070	ID=MS.gene25238.t1;Parent=MS.gene25238
chr7.1	exon	5914070	5914273	5914070	ID=MS.gene25238.t1.exon1;Parent=MS.gene25238.t1
chr7.1	CDS	5914070	5914273	5914070	ID=cds.MS.gene25238.t1;Parent=MS.gene25238.t1
chr7.1	exon	5914378	5914432	5914378	ID=MS.gene25238.t1.exon2;Parent=MS.gene25238.t1
chr7.1	CDS	5914378	5914432	5914378	ID=cds.MS.gene25238.t1;Parent=MS.gene25238.t1

Gene Sequence	>MS.gene25238 TGGTCCTAGAGAAGATGCAACCAGAATTGGATCTGCTGGAGTTGTTAGGAGGCAGGCTGTGGATATCTCTCCTCTTCGTCGGGTTAATCAGGCCATATATCTTCTAACAACTGGTGCCCGTGAAGCTGCATTTAGGAACATTAAGACAATTGCTGAATGCTTGGCTGATGAACTCATTAACGCTGCCAAGGGTTCATCCAACAGGTATACCACCATTATTCAATCTTTAAAGTTTCCAATGTACTGAATTTGTTTTCTCTTGGATTTTTCCTTGTTTGGTTCTTTGCTAACAAAATGTATCATTTCAGCTATGCGATCAAGAAAAAGGATGAGATTGAGAGAGTTGCCAAGGCCAATCGTTAA
Protein sequence	>MS.gene25238.t1 WSURRCNQNWICWSCUEAGCGYLSSSSGUSGHISSNNWCPUSCIUEHUDNCUMLGUUTHURCQGFIQQLCDQEKGUDUESCQGQSL