AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26122

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26122.t1 MTR_5g078030 98.347 242 4 0 1 242 37 278 9.24e-176 484
MS.gene26122.t1 MTR_3g065370 68.033 244 70 3 2 242 38 276 2.07e-112 324
MS.gene26122.t1 MTR_8g104990 51.220 246 113 2 2 240 35 280 1.54e-86 258
MS.gene26122.t1 MTR_1g080350 30.952 252 150 11 8 242 48 292 3.52e-23 95.5
MS.gene26122.t1 MTR_1g044580 31.020 245 160 5 4 241 48 290 6.12e-23 97.4
MS.gene26122.t1 MTR_7g098410 27.386 241 158 5 11 240 47 281 1.46e-22 93.6
MS.gene26122.t1 MTR_1g073320 28.627 255 161 6 4 240 46 297 6.79e-22 92.8
MS.gene26122.t1 MTR_2g088980 29.500 200 130 2 2 190 39 238 7.37e-22 90.9
MS.gene26122.t1 MTR_2g088930 28.358 201 133 4 2 191 39 239 6.59e-20 85.9
MS.gene26122.t1 MTR_5g005480 29.528 254 148 7 2 233 41 285 1.04e-18 85.1
MS.gene26122.t1 MTR_6g055960 26.389 216 119 3 3 190 34 237 8.68e-18 82.4
MS.gene26122.t1 MTR_1g105650 28.155 206 129 4 3 191 45 248 1.05e-16 79.3
MS.gene26122.t1 MTR_3g011890 25.389 193 133 2 8 190 47 238 1.24e-16 77.0
MS.gene26122.t1 MTR_1g105655 26.070 257 164 8 2 235 47 300 2.84e-16 78.2
MS.gene26122.t1 MTR_3g011910 25.490 204 133 4 4 192 55 254 1.48e-15 75.9
MS.gene26122.t1 MTR_1g104890 23.664 262 178 6 2 242 51 311 1.48e-15 75.1
MS.gene26122.t1 MTR_3g011910 25.490 204 133 4 4 192 29 228 1.80e-15 75.5
MS.gene26122.t1 MTR_8g041690 24.365 197 137 2 6 190 43 239 1.83e-15 75.5
MS.gene26122.t1 MTR_7g052020 26.768 198 122 5 10 191 42 232 2.42e-15 73.6
MS.gene26122.t1 MTR_1g104890 23.664 262 178 6 2 242 51 311 3.90e-15 74.3
MS.gene26122.t1 MTR_6g463400 26.733 202 133 3 4 190 42 243 6.04e-15 73.9
MS.gene26122.t1 MTR_1g104890 23.664 262 178 6 2 242 51 311 6.40e-15 73.9
MS.gene26122.t1 MTR_1g104890 23.664 262 178 6 2 242 51 311 7.80e-15 73.6
MS.gene26122.t1 MTR_5g068260 26.341 205 132 4 2 191 39 239 5.24e-14 71.2
MS.gene26122.t1 MTR_5g005450 28.042 189 107 3 2 169 41 221 1.01e-13 70.5
MS.gene26122.t1 MTR_1g021610 26.316 209 133 4 2 193 44 248 1.16e-13 70.5
MS.gene26122.t1 MTR_1g021635 27.619 210 131 7 1 192 41 247 1.16e-13 70.5
MS.gene26122.t1 MTR_6g463710 28.230 209 124 5 3 190 42 245 1.35e-13 70.1
MS.gene26122.t1 MTR_6g057770 28.230 209 124 5 3 190 42 245 1.35e-13 70.1
MS.gene26122.t1 MTR_6g053000 26.404 178 117 4 3 168 39 214 3.21e-13 68.9
MS.gene26122.t1 MTR_5g068250 26.214 206 133 4 2 192 44 245 4.17e-13 68.6
MS.gene26122.t1 MTR_7g033135 32.692 156 97 4 42 192 80 232 5.05e-13 68.6
MS.gene26122.t1 MTR_8g041710 22.925 253 178 5 5 241 43 294 1.07e-12 67.4
MS.gene26122.t1 MTR_8g041650 25.749 167 110 3 8 162 50 214 2.24e-12 66.6
MS.gene26122.t1 MTR_1g105615 25.837 209 133 5 2 191 42 247 2.85e-12 66.2
MS.gene26122.t1 MTR_8g041880 26.344 186 124 2 3 175 41 226 2.98e-12 66.2
MS.gene26122.t1 MTR_5g068190 26.667 210 132 5 2 192 37 243 7.17e-12 65.1
MS.gene26122.t1 MTR_0007s0020 26.316 209 131 5 3 190 39 245 1.52e-11 64.3
MS.gene26122.t1 MTR_8g028065 26.136 176 124 1 21 190 61 236 3.45e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26122.t1 AT5G37660 62.810 242 89 1 2 242 47 288 7.67e-101 295
MS.gene26122.t1 AT5G37660 62.656 241 89 1 2 241 47 287 5.62e-100 293
MS.gene26122.t1 AT2G01660 55.870 247 100 4 2 239 37 283 4.99e-91 270
MS.gene26122.t1 AT2G01660 55.870 247 100 4 2 239 37 283 4.99e-91 270
MS.gene26122.t1 AT2G01660 59.615 208 80 3 2 205 37 244 4.67e-83 249
MS.gene26122.t1 AT1G70690 45.418 251 124 4 2 239 41 291 2.44e-66 207
MS.gene26122.t1 AT3G60720 39.095 243 142 3 2 239 39 280 8.36e-53 172
MS.gene26122.t1 AT3G60720 39.669 242 136 4 2 238 39 275 9.99e-53 172
MS.gene26122.t1 AT3G60720 39.130 207 121 2 2 203 39 245 6.15e-46 154
MS.gene26122.t1 AT1G04520 27.481 262 159 4 4 240 48 303 1.19e-20 89.0
MS.gene26122.t1 AT2G33330 26.877 253 168 3 4 240 49 300 1.46e-20 88.6
MS.gene26122.t1 AT5G48540 27.000 200 131 3 5 190 37 235 1.55e-18 82.4
MS.gene26122.t1 AT5G43980 28.077 260 165 6 4 242 41 299 4.07e-18 82.0
MS.gene26122.t1 AT1G63550 26.570 207 135 4 2 193 42 246 2.29e-17 80.1
MS.gene26122.t1 AT1G63580 25.210 238 153 7 8 222 37 272 1.66e-15 74.3
MS.gene26122.t1 AT3G22060 30.542 203 122 6 2 190 46 243 1.20e-14 71.6
MS.gene26122.t1 AT5G41290 24.883 213 127 6 3 192 41 243 2.26e-14 71.2
MS.gene26122.t1 AT5G40380 29.353 201 118 7 42 231 84 271 9.81e-14 70.5
MS.gene26122.t1 AT5G40380 29.353 201 118 7 42 231 84 271 1.17e-13 70.5
MS.gene26122.t1 AT5G40380 29.353 201 118 7 42 231 84 271 1.20e-13 70.5
MS.gene26122.t1 AT5G41300 23.645 203 138 3 3 190 45 245 2.05e-13 68.6
MS.gene26122.t1 AT4G23310 28.440 218 138 5 1 203 38 252 3.54e-13 68.9
MS.gene26122.t1 AT4G23310 28.440 218 138 5 1 203 38 252 4.67e-13 68.6
MS.gene26122.t1 AT1G63570 26.794 209 130 5 2 192 38 241 6.98e-13 67.0
MS.gene26122.t1 AT1G63570 26.794 209 130 5 2 192 38 241 6.98e-13 67.0
MS.gene26122.t1 AT1G61750 20.755 265 174 5 2 231 40 303 7.20e-13 67.4
MS.gene26122.t1 AT1G63570 26.794 209 130 5 2 192 91 294 1.22e-12 66.6
MS.gene26122.t1 AT3G22010 25.581 215 126 9 2 191 51 256 3.66e-12 64.7
MS.gene26122.t1 AT3G21990 25.962 208 133 7 2 191 45 249 1.34e-11 63.2
MS.gene26122.t1 AT4G23280 26.432 227 137 8 2 204 39 259 1.42e-11 64.3
MS.gene26122.t1 AT4G23180 29.843 191 106 7 2 172 50 232 1.44e-11 64.3
MS.gene26122.t1 AT4G23280 26.432 227 137 8 2 204 39 259 1.49e-11 64.3
MS.gene26122.t1 AT4G23180 29.843 191 106 7 2 172 50 232 1.51e-11 63.9
MS.gene26122.t1 AT4G23280 26.432 227 137 8 2 204 39 259 1.80e-11 63.9
MS.gene26122.t1 AT4G23180 29.843 191 106 7 2 172 50 232 2.20e-11 63.5
MS.gene26122.t1 AT4G20640 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene26122.t1 AT4G20580 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene26122.t1 AT4G20610 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene26122.t1 AT4G20630 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene26122.t1 AT4G20620 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene26122.t1 AT4G20590 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene26122.t1 AT4G20600 24.155 207 138 5 2 191 45 249 5.35e-11 61.2
MS.gene26122.t1 AT4G20570 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene26122.t1 AT4G20560 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene26122.t1 AT4G20645 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene26122.t1 AT4G20550 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene26122.t1 AT4G20530 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene26122.t1 AT4G20540 24.155 207 138 5 2 191 59 263 6.75e-11 61.2
MS.gene26122.t1 AT4G20670 24.155 207 138 5 2 191 45 249 8.83e-11 60.8

Find 78 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTGAGCCCATGGCTTC+TGG 0.211124 5.4:+56921018 None:intergenic
TGTACTGGGGATCTTAGTTT+TGG 0.219626 5.4:-56920916 MS.gene26122:CDS
CCCGAACCACCAACCCTAAA+AGG 0.258817 5.4:+56920961 None:intergenic
TGGATGGCTTGCAGGAAAAT+TGG 0.277770 5.4:-56920003 MS.gene26122:intron
CTTTCTTGAGCAAGATTTGT+AGG 0.299010 5.4:-56919905 MS.gene26122:CDS
CTTGGTGGGCTTGTGGGCTT+AGG 0.300238 5.4:-56920988 MS.gene26122:CDS
CGTGGTGTTGCCCAGTGTAC+TGG 0.317322 5.4:-56920931 MS.gene26122:CDS
GCTGTGCTTGGTGGGCTTGT+GGG 0.350767 5.4:-56920994 MS.gene26122:CDS
GATTGTGGTACTGCAGATTA+TGG 0.358387 5.4:-56920847 MS.gene26122:CDS
GCACGCGCTGTCACACGTGC+TGG 0.363096 5.4:-56921177 MS.gene26122:CDS
AAGAGATTTGCATTAATCAT+TGG 0.369856 5.4:-56919966 MS.gene26122:CDS
GATTATGGAGATTTGTTCTT+GGG 0.375248 5.4:-56920832 MS.gene26122:CDS
AGCAATGAGAATAACAAGTA+TGG 0.392517 5.4:+56919926 None:intergenic
TTTGACTCGTAGGGTGAGTT+TGG 0.395292 5.4:+56921351 None:intergenic
TGCTGTGCTTGGTGGGCTTG+TGG 0.401298 5.4:-56920995 MS.gene26122:CDS
GGTGGGCTTGTGGGCTTAGG+TGG 0.405467 5.4:-56920985 MS.gene26122:CDS
TTAATCATTGGTTTATTAGC+AGG 0.406034 5.4:-56919954 MS.gene26122:CDS
TCAAGAGATGCTGTGCTTGG+TGG 0.408146 5.4:-56921003 MS.gene26122:CDS
GGTCGCCACGGCATTGATAT+AGG 0.413623 5.4:+56921229 None:intergenic
GGTCCAGCTCGACGGTTGTT+TGG 0.415998 5.4:-56921124 MS.gene26122:CDS
GGCCATAGATTATGTCTTGT+TGG 0.417754 5.4:+56921250 None:intergenic
AGATTATGGAGATTTGTTCT+TGG 0.419653 5.4:-56920833 MS.gene26122:CDS
CAAGAGATGCTGTGCTTGGT+GGG 0.419763 5.4:-56921002 MS.gene26122:CDS
GTGGGCTTGTGGGCTTAGGT+GGG 0.426219 5.4:-56920984 MS.gene26122:CDS
GCCATAGATTATGTCTTGTT+GGG 0.429320 5.4:+56921251 None:intergenic
TAGGGTTGGTGGTTCGGGTA+TGG 0.430373 5.4:-56920956 MS.gene26122:CDS
TGGGGCCCATGATACGTCTA+AGG 0.432532 5.4:-56920782 MS.gene26122:CDS
CACGCGCTGTCACACGTGCT+GGG 0.445092 5.4:-56921176 MS.gene26122:CDS
ACCCAACAAGACATAATCTA+TGG 0.445749 5.4:-56921252 MS.gene26122:CDS
GTGGTGTTGCCCAGTGTACT+GGG 0.465182 5.4:-56920930 MS.gene26122:CDS
TACAACAACTTCACCGTCCT+AGG 0.469752 5.4:-56921279 MS.gene26122:CDS
GCAGGAAAATTGGGTATAGT+AGG 0.470565 5.4:-56919993 MS.gene26122:intron
CATGGGCCTTAGACGTATCA+TGG 0.471929 5.4:+56920776 None:intergenic
GGCTCAAGAGATGCTGTGCT+TGG 0.472019 5.4:-56921006 MS.gene26122:CDS
GTGAGGTGAGGAGTGAATTG+AGG 0.473079 5.4:+56921328 None:intergenic
GGATGGCTTGCAGGAAAATT+GGG 0.474228 5.4:-56920002 MS.gene26122:intron
TCGCGACTCGTGCGGAGGCA+CGG 0.481840 5.4:-56921145 MS.gene26122:CDS
TGTCTTGTTGGGTGGACCCT+AGG 0.483605 5.4:+56921262 None:intergenic
TGAATTGAGGTTTGACTCGT+AGG 0.499158 5.4:+56921341 None:intergenic
CCGTGGCGACCTCGCTATGC+CGG 0.500432 5.4:-56921217 MS.gene26122:CDS
CACAGCATCTCTTGAGCCCA+TGG 0.506789 5.4:+56921011 None:intergenic
AACTAAGATCCCCAGTACAC+TGG 0.510980 5.4:+56920920 None:intergenic
TAGGACGGTGAAGTTGTTGT+AGG 0.515242 5.4:+56921281 None:intergenic
TCACCAAACAACCGTCGAGC+TGG 0.516521 5.4:+56921121 None:intergenic
ATAGATTATGTCTTGTTGGG+TGG 0.527972 5.4:+56921254 None:intergenic
GGGCTATAATCCAGAAGCCA+TGG 0.539733 5.4:-56921028 MS.gene26122:CDS
ACTAAGATCCCCAGTACACT+GGG 0.557663 5.4:+56920921 None:intergenic
GCGACACAGGAGGCACAGTC+CGG 0.560545 5.4:+56921198 None:intergenic
TGTGACAGCGCGTGCGACAC+AGG 0.562753 5.4:+56921185 None:intergenic
GGCTATAATCCAGAAGCCAT+GGG 0.566857 5.4:-56921027 MS.gene26122:CDS
AGACGTATCATGGGCCCCAC+CGG 0.577254 5.4:+56920786 None:intergenic
ATGCAAGATATTCTACCGGT+GGG 0.580075 5.4:-56920801 MS.gene26122:CDS
GTGTTGTAGAAGCGATCGGA+CGG 0.581568 5.4:-56920879 MS.gene26122:CDS
GAATTGAGGTTTGACTCGTA+GGG 0.593749 5.4:+56921342 None:intergenic
ACAACAACTTCACCGTCCTA+GGG 0.596633 5.4:-56921278 MS.gene26122:CDS
GGAGGCACGGTCCAGCTCGA+CGG 0.596985 5.4:-56921132 MS.gene26122:CDS
GGTGGTTCGGGTATGGTACG+TGG 0.597946 5.4:-56920949 MS.gene26122:CDS
AGCAAGATTTGTAGGAGACA+AGG 0.603122 5.4:-56919897 MS.gene26122:intron
ACGCGCTGTCACACGTGCTG+GGG 0.608382 5.4:-56921175 MS.gene26122:CDS
CCGGCATAGCGAGGTCGCCA+CGG 0.614398 5.4:+56921217 None:intergenic
ATGTGTCGCGACTCGTGCGG+AGG 0.615055 5.4:-56921150 MS.gene26122:CDS
GGCTTCTGGATTATAGCCCA+CGG 0.615132 5.4:+56921032 None:intergenic
GGACGGTTGAAAAGTGATTG+TGG 0.615271 5.4:-56920862 MS.gene26122:CDS
TGTTATGCAAGATATTCTAC+CGG 0.616220 5.4:-56920805 MS.gene26122:CDS
AGGCACAGTCCGGCATAGCG+AGG 0.619370 5.4:+56921208 None:intergenic
GATTGTGTTGTAGAAGCGAT+CGG 0.619387 5.4:-56920883 MS.gene26122:CDS
TATGCAAGATATTCTACCGG+TGG 0.627330 5.4:-56920802 MS.gene26122:CDS
CATGATACGTCTAAGGCCCA+TGG 0.629745 5.4:-56920775 MS.gene26122:intron
ATGGGCCTTAGACGTATCAT+GGG 0.630265 5.4:+56920777 None:intergenic
TCTGGATTATAGCCCACGGA+AGG 0.644583 5.4:+56921036 None:intergenic
TGCAAGATATTCTACCGGTG+GGG 0.653008 5.4:-56920800 MS.gene26122:CDS
TTGTTGGGTGGACCCTAGGA+CGG 0.669929 5.4:+56921266 None:intergenic
CTGAGTTGACTAGTGAGGTG+AGG 0.674733 5.4:+56921316 None:intergenic
GACATGTGTCGCGACTCGTG+CGG 0.678683 5.4:-56921153 MS.gene26122:CDS
TATGGCCTATATCAATGCCG+TGG 0.684424 5.4:-56921234 MS.gene26122:CDS
GACAGCGCGTGCGACACAGG+AGG 0.694407 5.4:+56921188 None:intergenic
TGGTGTTGCCCAGTGTACTG+GGG 0.727512 5.4:-56920929 MS.gene26122:CDS
TGTTGCTGAGTTGACTAGTG+AGG 0.747592 5.4:+56921311 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAAACATATAACCAT+TGG - chr5.4:56921297-56921316 MS.gene26122:CDS 15.0%
!! AATGTTTACAAATTACAAAT+TGG + chr5.4:56920622-56920641 None:intergenic 15.0%
!! ATGTTGAAATAATAAAACAT+TGG + chr5.4:56920699-56920718 None:intergenic 15.0%
!! ATTTACTTGTGAAAATATTA+GGG - chr5.4:56920777-56920796 MS.gene26122:CDS 15.0%
!! TATTTACTTGTGAAAATATT+AGG - chr5.4:56920776-56920795 MS.gene26122:CDS 15.0%
!! TGAATTGATTATATTAGTTA+AGG + chr5.4:56921268-56921287 None:intergenic 15.0%
!! TGTTGAAATAATAAAACATT+GGG + chr5.4:56920698-56920717 None:intergenic 15.0%
!! AGAGATTTGTAAGAAAAAAA+AGG - chr5.4:56920726-56920745 MS.gene26122:intron 20.0%
!!! ATGTTTTCTTTTACTTTGTT+AGG - chr5.4:56920557-56920576 MS.gene26122:intron 20.0%
!!! CTACTTCTTTTTTAATCAAA+AGG - chr5.4:56920383-56920402 MS.gene26122:intron 20.0%
!!! TGTTTTCTTTTACTTTGTTA+GGG - chr5.4:56920558-56920577 MS.gene26122:intron 20.0%
!!! TTTTTTCTTACAAATCTCTT+TGG + chr5.4:56920725-56920744 None:intergenic 20.0%
! AAGAGATTTGCATTAATCAT+TGG - chr5.4:56921126-56921145 MS.gene26122:CDS 25.0%
! ACATGTGATCAAAATTCAAA+GGG + chr5.4:56921043-56921062 None:intergenic 25.0%
! AGGAATTAATTGAAGAGTAA+TGG - chr5.4:56920403-56920422 MS.gene26122:intron 25.0%
! TTACAAATTACAAATTGGCA+TGG + chr5.4:56920617-56920636 None:intergenic 25.0%
!! GTAATTTGTAAACATTGACT+TGG - chr5.4:56920626-56920645 MS.gene26122:intron 25.0%
!!! AATGTTTTGTTGAAAAAGTG+TGG - chr5.4:56920032-56920051 MS.gene26122:intron 25.0%
!!! AATTACCTGACTTATTTTGT+TGG - chr5.4:56921069-56921088 MS.gene26122:CDS 25.0%
!!! ATGACTTTTTTTCTTTTGAC+CGG + chr5.4:56920539-56920558 None:intergenic 25.0%
!!! ATGTTTTGTTGAAAAAGTGT+GGG - chr5.4:56920033-56920052 MS.gene26122:intron 25.0%
!!! TTAATCATTGGTTTATTAGC+AGG - chr5.4:56921138-56921157 MS.gene26122:CDS 25.0%
!!! TTTCAAGTATGTTTTTCTTC+CGG - chr5.4:56920517-56920536 MS.gene26122:intron 25.0%
AGCAATGAGAATAACAAGTA+TGG + chr5.4:56921169-56921188 None:intergenic 30.0%
CAAGATATTGCTTAATCCAA+TGG + chr5.4:56921316-56921335 None:intergenic 30.0%
CACATGTGATCAAAATTCAA+AGG + chr5.4:56921044-56921063 None:intergenic 30.0%
TGTTATGCAAGATATTCTAC+CGG - chr5.4:56920287-56920306 MS.gene26122:intron 30.0%
! AATTGAAGAGTAATGGAGAT+TGG - chr5.4:56920410-56920429 MS.gene26122:intron 30.0%
! AGATTATGGAGATTTGTTCT+TGG - chr5.4:56920259-56920278 MS.gene26122:intron 30.0%
! ATTGAAGAGTAATGGAGATT+GGG - chr5.4:56920411-56920430 MS.gene26122:intron 30.0%
! GATTATGGAGATTTGTTCTT+GGG - chr5.4:56920260-56920279 MS.gene26122:intron 30.0%
!! CTTTGAATTTTGATCACATG+TGG - chr5.4:56921042-56921061 MS.gene26122:CDS 30.0%
!!! TATGACAATGCTACTTTTCT+AGG - chr5.4:56919996-56920015 MS.gene26122:intron 30.0%
AAAAGAAGTAGAAGCAGCAA+AGG + chr5.4:56920374-56920393 None:intergenic 35.0%
ACCCAACAAGACATAATCTA+TGG - chr5.4:56919840-56919859 MS.gene26122:intron 35.0%
CTTTCTTGAGCAAGATTTGT+AGG - chr5.4:56921187-56921206 MS.gene26122:CDS 35.0%
GAGACAAGGTAAGTAGTATT+AGG - chr5.4:56921209-56921228 MS.gene26122:CDS 35.0%
! ATAGATTATGTCTTGTTGGG+TGG + chr5.4:56919841-56919860 None:intergenic 35.0%
! GCCATAGATTATGTCTTGTT+GGG + chr5.4:56919844-56919863 None:intergenic 35.0%
!! AAACATTGACTTGGAGAATG+TGG - chr5.4:56920635-56920654 MS.gene26122:intron 35.0%
!!! ACCTGACTTATTTTGTTGGA+TGG - chr5.4:56921073-56921092 MS.gene26122:CDS 35.0%
ACATGAGAAAGAACCCAAGA+TGG + chr5.4:56920349-56920368 None:intergenic 40.0%
AGCAAGATTTGTAGGAGACA+AGG - chr5.4:56921195-56921214 MS.gene26122:CDS 40.0%
ATGCAAGATATTCTACCGGT+GGG - chr5.4:56920291-56920310 MS.gene26122:intron 40.0%
CATGAGAAAGAACCCAAGAT+GGG + chr5.4:56920348-56920367 None:intergenic 40.0%
GATTGTGGTACTGCAGATTA+TGG - chr5.4:56920245-56920264 MS.gene26122:intron 40.0%
GATTGTGTTGTAGAAGCGAT+CGG - chr5.4:56920209-56920228 MS.gene26122:intron 40.0%
GCAGGAAAATTGGGTATAGT+AGG - chr5.4:56921099-56921118 MS.gene26122:CDS 40.0%
GCCATCCAACAAAATAAGTC+AGG + chr5.4:56921077-56921096 None:intergenic 40.0%
GGCCATAGATTATGTCTTGT+TGG + chr5.4:56919845-56919864 None:intergenic 40.0%
TATGCAAGATATTCTACCGG+TGG - chr5.4:56920290-56920309 MS.gene26122:intron 40.0%
! GAATTGAGGTTTGACTCGTA+GGG + chr5.4:56919753-56919772 None:intergenic 40.0%
! TGAATTGAGGTTTGACTCGT+AGG + chr5.4:56919754-56919773 None:intergenic 40.0%
! TGTACTGGGGATCTTAGTTT+TGG - chr5.4:56920176-56920195 MS.gene26122:intron 40.0%
!! GTACTGGGGATCTTAGTTTT+GGG - chr5.4:56920177-56920196 MS.gene26122:intron 40.0%
!! TATTTTGTTGGATGGCTTGC+AGG - chr5.4:56921081-56921100 MS.gene26122:CDS 40.0%
!!! ACAGCGTTTGTCGTTTTTTC+AGG - chr5.4:56920582-56920601 MS.gene26122:intron 40.0%
!!! CAATGCTACTTTTCTAGGAG+TGG - chr5.4:56920001-56920020 MS.gene26122:intron 40.0%
!!! CTTGTATCTTTTTTCCGTGC+AGG - chr5.4:56921353-56921372 MS.gene26122:CDS 40.0%
!!! TACTGGGGATCTTAGTTTTG+GGG - chr5.4:56920178-56920197 MS.gene26122:intron 40.0%
AACTAAGATCCCCAGTACAC+TGG + chr5.4:56920175-56920194 None:intergenic 45.0%
ACAACAACTTCACCGTCCTA+GGG - chr5.4:56919814-56919833 MS.gene26122:intron 45.0%
ACTAAGATCCCCAGTACACT+GGG + chr5.4:56920174-56920193 None:intergenic 45.0%
ATGGGCCTTAGACGTATCAT+GGG + chr5.4:56920318-56920337 None:intergenic 45.0%
GGACGGTTGAAAAGTGATTG+TGG - chr5.4:56920230-56920249 MS.gene26122:intron 45.0%
GGCTATAATCCAGAAGCCAT+GGG - chr5.4:56920065-56920084 MS.gene26122:intron 45.0%
TACAACAACTTCACCGTCCT+AGG - chr5.4:56919813-56919832 MS.gene26122:intron 45.0%
TAGGACGGTGAAGTTGTTGT+AGG + chr5.4:56919814-56919833 None:intergenic 45.0%
TATGGCCTATATCAATGCCG+TGG - chr5.4:56919858-56919877 MS.gene26122:intron 45.0%
TGCAAGATATTCTACCGGTG+GGG - chr5.4:56920292-56920311 MS.gene26122:intron 45.0%
! GAAGAGTAATGGAGATTGGG+TGG - chr5.4:56920414-56920433 MS.gene26122:intron 45.0%
! GGATGGCTTGCAGGAAAATT+GGG - chr5.4:56921090-56921109 MS.gene26122:CDS 45.0%
! TGGATGGCTTGCAGGAAAAT+TGG - chr5.4:56921089-56921108 MS.gene26122:CDS 45.0%
! TGTTGCTGAGTTGACTAGTG+AGG + chr5.4:56919784-56919803 None:intergenic 45.0%
! TTTGACTCGTAGGGTGAGTT+TGG + chr5.4:56919744-56919763 None:intergenic 45.0%
!!! TGCTACTTTTCTAGGAGTGG+AGG - chr5.4:56920004-56920023 MS.gene26122:intron 45.0%
CAAGAGATGCTGTGCTTGGT+GGG - chr5.4:56920090-56920109 MS.gene26122:intron 50.0%
CATGGGCCTTAGACGTATCA+TGG + chr5.4:56920319-56920338 None:intergenic 50.0%
CCCATGGTAAAGCCCATCTT+GGG - chr5.4:56920333-56920352 MS.gene26122:intron 50.0%
GGCTTCTGGATTATAGCCCA+CGG + chr5.4:56920063-56920082 None:intergenic 50.0%
GGGCTATAATCCAGAAGCCA+TGG - chr5.4:56920064-56920083 MS.gene26122:intron 50.0%
GTGAGGTGAGGAGTGAATTG+AGG + chr5.4:56919767-56919786 None:intergenic 50.0%
GTGTTGTAGAAGCGATCGGA+CGG - chr5.4:56920213-56920232 MS.gene26122:intron 50.0%
TCAAGAGATGCTGTGCTTGG+TGG - chr5.4:56920089-56920108 MS.gene26122:intron 50.0%
TCTGGATTATAGCCCACGGA+AGG + chr5.4:56920059-56920078 None:intergenic 50.0%
! AAAAAGTGTGGGCCTTCCGT+GGG - chr5.4:56920044-56920063 MS.gene26122:intron 50.0%
! ACCCAAGATGGGCTTTACCA+TGG + chr5.4:56920337-56920356 None:intergenic 50.0%
! CATGATACGTCTAAGGCCCA+TGG - chr5.4:56920317-56920336 MS.gene26122:intron 50.0%
! CCCAAGATGGGCTTTACCAT+GGG + chr5.4:56920336-56920355 None:intergenic 50.0%
! CTGAGTTGACTAGTGAGGTG+AGG + chr5.4:56919779-56919798 None:intergenic 50.0%
! GAGTAATGGAGATTGGGTGG+TGG - chr5.4:56920417-56920436 MS.gene26122:intron 50.0%
!!! CCTTTTAGGGTTGGTGGTTC+GGG - chr5.4:56920131-56920150 MS.gene26122:intron 50.0%
!!! GCCTTTTAGGGTTGGTGGTT+CGG - chr5.4:56920130-56920149 MS.gene26122:intron 50.0%
!!! TTAGGTGGGCCTTTTAGGGT+TGG - chr5.4:56920122-56920141 MS.gene26122:intron 50.0%
CACAGCATCTCTTGAGCCCA+TGG + chr5.4:56920084-56920103 None:intergenic 55.0%
CCCGAACCACCAACCCTAAA+AGG + chr5.4:56920134-56920153 None:intergenic 55.0%
GCCCATGGTAAAGCCCATCT+TGG - chr5.4:56920332-56920351 MS.gene26122:intron 55.0%
GGCTCAAGAGATGCTGTGCT+TGG - chr5.4:56920086-56920105 MS.gene26122:intron 55.0%
TCACCAAACAACCGTCGAGC+TGG + chr5.4:56919974-56919993 None:intergenic 55.0%
TCTCTTGAGCCCATGGCTTC+TGG + chr5.4:56920077-56920096 None:intergenic 55.0%
TGGGGCCCATGATACGTCTA+AGG - chr5.4:56920310-56920329 MS.gene26122:intron 55.0%
TTGTTGGGTGGACCCTAGGA+CGG + chr5.4:56919829-56919848 None:intergenic 55.0%
! GAAAAAGTGTGGGCCTTCCG+TGG - chr5.4:56920043-56920062 MS.gene26122:intron 55.0%
! GGGCTTAGGTGGGCCTTTTA+GGG - chr5.4:56920118-56920137 MS.gene26122:intron 55.0%
! TGGGCTTAGGTGGGCCTTTT+AGG - chr5.4:56920117-56920136 MS.gene26122:intron 55.0%
! TGTCTTGTTGGGTGGACCCT+AGG + chr5.4:56919833-56919852 None:intergenic 55.0%
!! GGTCGCCACGGCATTGATAT+AGG + chr5.4:56919866-56919885 None:intergenic 55.0%
!! GTGGTGTTGCCCAGTGTACT+GGG - chr5.4:56920162-56920181 MS.gene26122:intron 55.0%
!! TAGGGTTGGTGGTTCGGGTA+TGG - chr5.4:56920136-56920155 MS.gene26122:intron 55.0%
!! TGGTGTTGCCCAGTGTACTG+GGG - chr5.4:56920163-56920182 MS.gene26122:intron 55.0%
AGACGTATCATGGGCCCCAC+CGG + chr5.4:56920309-56920328 None:intergenic 60.0%
GACATGTGTCGCGACTCGTG+CGG - chr5.4:56919939-56919958 MS.gene26122:CDS 60.0%
GGTCCAGCTCGACGGTTGTT+TGG - chr5.4:56919968-56919987 MS.gene26122:CDS 60.0%
GTGGGCTTGTGGGCTTAGGT+GGG - chr5.4:56920108-56920127 MS.gene26122:intron 60.0%
! GGTGGTTCGGGTATGGTACG+TGG - chr5.4:56920143-56920162 MS.gene26122:intron 60.0%
!! CGTGGTGTTGCCCAGTGTAC+TGG - chr5.4:56920161-56920180 MS.gene26122:intron 60.0%
!! CTTGGTGGGCTTGTGGGCTT+AGG - chr5.4:56920104-56920123 MS.gene26122:intron 60.0%
!! GCTGTGCTTGGTGGGCTTGT+GGG - chr5.4:56920098-56920117 MS.gene26122:intron 60.0%
!! TGCTGTGCTTGGTGGGCTTG+TGG - chr5.4:56920097-56920116 MS.gene26122:intron 60.0%
!!! GGTGGGCCTTTTAGGGTTGG+TGG - chr5.4:56920125-56920144 MS.gene26122:intron 60.0%
ACGCGCTGTCACACGTGCTG+GGG - chr5.4:56919917-56919936 MS.gene26122:CDS 65.0%
AGGCACAGTCCGGCATAGCG+AGG + chr5.4:56919887-56919906 None:intergenic 65.0%
ATGTGTCGCGACTCGTGCGG+AGG - chr5.4:56919942-56919961 MS.gene26122:CDS 65.0%
CACGCGCTGTCACACGTGCT+GGG - chr5.4:56919916-56919935 MS.gene26122:CDS 65.0%
TGTGACAGCGCGTGCGACAC+AGG + chr5.4:56919910-56919929 None:intergenic 65.0%
! GCGACACAGGAGGCACAGTC+CGG + chr5.4:56919897-56919916 None:intergenic 65.0%
! GGTGGGCTTGTGGGCTTAGG+TGG - chr5.4:56920107-56920126 MS.gene26122:intron 65.0%
CCGGCATAGCGAGGTCGCCA+CGG + chr5.4:56919878-56919897 None:intergenic 70.0%
CCGTGGCGACCTCGCTATGC+CGG - chr5.4:56919875-56919894 MS.gene26122:intron 70.0%
GACAGCGCGTGCGACACAGG+AGG + chr5.4:56919907-56919926 None:intergenic 70.0%
GCACGCGCTGTCACACGTGC+TGG - chr5.4:56919915-56919934 MS.gene26122:CDS 70.0%
TCGCGACTCGTGCGGAGGCA+CGG - chr5.4:56919947-56919966 MS.gene26122:CDS 70.0%
! GGAGGCACGGTCCAGCTCGA+CGG - chr5.4:56919960-56919979 MS.gene26122:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 56919732 56921382 56919732 ID=MS.gene26122
chr5.4 mRNA 56919732 56921382 56919732 ID=MS.gene26122.t1;Parent=MS.gene26122
chr5.4 exon 56920776 56921382 56920776 ID=MS.gene26122.t1.exon1;Parent=MS.gene26122.t1
chr5.4 CDS 56920776 56921382 56920776 ID=cds.MS.gene26122.t1;Parent=MS.gene26122.t1
chr5.4 exon 56919898 56920011 56919898 ID=MS.gene26122.t1.exon2;Parent=MS.gene26122.t1
chr5.4 CDS 56919898 56920011 56919898 ID=cds.MS.gene26122.t1;Parent=MS.gene26122.t1
chr5.4 exon 56919732 56919739 56919732 ID=MS.gene26122.t1.exon3;Parent=MS.gene26122.t1
chr5.4 CDS 56919732 56919739 56919732 ID=cds.MS.gene26122.t1;Parent=MS.gene26122.t1
Gene Sequence

>MS.gene26122

ATGTACACACCAAACTCACCCTACGAGTCAAACCTCAATTCACTCCTCACCTCACTAGTCAACTCAGCAACATACTCATCCTACAACAACTTCACCGTCCTAGGGTCCACCCAACAAGACATAATCTATGGCCTATATCAATGCCGTGGCGACCTCGCTATGCCGGACTGTGCCTCCTGTGTCGCACGCGCTGTCACACGTGCTGGGGACATGTGTCGCGACTCGTGCGGAGGCACGGTCCAGCTCGACGGTTGTTTGGTGAAGTATGACAATGCTACTTTTCTAGGAGTGGAGGATAAGAATGTTTTGTTGAAAAAGTGTGGGCCTTCCGTGGGCTATAATCCAGAAGCCATGGGCTCAAGAGATGCTGTGCTTGGTGGGCTTGTGGGCTTAGGTGGGCCTTTTAGGGTTGGTGGTTCGGGTATGGTACGTGGTGTTGCCCAGTGTACTGGGGATCTTAGTTTTGGGGAGTGTCAAGATTGTGTTGTAGAAGCGATCGGACGGTTGAAAAGTGATTGTGGTACTGCAGATTATGGAGATTTGTTCTTGGGTAAATGTTATGCAAGATATTCTACCGGTGGGGCCCATGATACGTCTAAGGCCCATGGTAAAGCCCATCTTGGGTTCTTTCTCATGTTACCTTTGCTGCTTCTACTTCTTTTTTAATCAAAAGGAATTAATTGAAGAGTAATGGAGATTGGGTGGTGGTGTAACTTAATTTGAATTGTAAATTTGTTTTTGATCAACTTTTTTTTTTTTTCATTTGCAAACTGTAATATGCAAAATTTCAAGTATGTTTTTCTTCCGGTCAAAAGAAAAAAAGTCATGTTTTCTTTTACTTTGTTAGGGCACAGCGTTTGTCGTTTTTTCAGGATGATTCTTCCATGCCAATTTGTAATTTGTAAACATTGACTTGGAGAATGTGGTAGATTTAACTAAAATAAGTAAAAATATATATATATACCCAATGTTTTATTATTTCAACATGTTCCAAAGAGATTTGTAAGAAAAAAAAGGAACACACTTATTTTTCTTCTAATAGTTTATTTACTTGTGAAAATATTAGGGTTTGTTCTTTTATTAAAATATAAAAACATATTATGTTTTGTTATGCATATATTTTATTTGTACATTTTTTACTTTAATTGATATTACTTTGTTTCAGTTTTATAACATTCATATTATGCATGTATATAAATTTTAGTTTCAATATTCTAGTTGTATGTTTTTAAATTCAATTTTTTTTTTTAAATTATGTTATCAAAATAAAATTTATAGTACAAAAATATAATCAAAGTTGATGTTATTCCCTTTGAATTTTGATCACATGTGGTATAAATTACCTGACTTATTTTGTTGGATGGCTTGCAGGAAAATTGGGTATAGTAGGTGAAAAGAGATTTGCATTAATCATTGGTTTATTAGCAGGAGCAGCCATACTTGTTATTCTCATTGCTTTCTTGAGCAAGATTTGTAGGAGACAAGGTAAGTAGTATTAGGCTATTTCTTTTCTTTTTTTGTCTCTTTGATTTTCCTTAACTAATATAATCAATTCAGATCATAAAAAATTAAAACATATAACCATTGGATTAAGCAATATCTTGACATGATTTTTTTTGTGCTTGTATCTTTTTTCCGTGCAGGTAAATAA
Protein sequence

>MS.gene26122.t1

MYTPNSPYESNLNSLLTSLVNSATYSSYNNFTVLGSTQQDIIYGLYQCRGDLAMPDCASCVARAVTRAGDMCRDSCGGTVQLDGCLVKYDNATFLGVEDKNVLLKKCGPSVGYNPEAMGSRDAVLGGLVGLGGPFRVGGSGMVRGVAQCTGDLSFGECQDCVVEAIGRLKSDCGTADYGDLFLGKCYARYSTGGAHDTSKAHGKLGIVGEKRFALIIGLLAGAAILVILIAFLSKICRRQGK