AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054850


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054850.t1 MTR_7g089400 87.200 125 15 1 1 125 1 124 1.78e-74 223
MS.gene054850.t1 MTR_1g093750 49.215 191 90 3 59 243 141 330 3.96e-47 159
MS.gene054850.t1 MTR_3g070970 35.714 252 136 6 14 243 7 254 3.42e-42 144
MS.gene054850.t1 MTR_2g091190 45.810 179 90 3 71 243 54 231 4.37e-33 120
MS.gene054850.t1 MTR_2g015890 38.068 176 100 3 71 241 62 233 5.07e-30 113
MS.gene054850.t1 MTR_4g079760 42.553 188 91 5 68 243 60 242 3.49e-28 108
MS.gene054850.t1 MTR_2g015890 41.880 117 64 1 71 187 62 174 2.06e-24 97.4
MS.gene054850.t1 MTR_5g066080 38.506 174 79 3 59 206 19 190 7.14e-23 94.0
MS.gene054850.t1 MTR_5g032375 32.558 215 117 6 32 233 7 206 4.87e-22 90.9
MS.gene054850.t1 MTR_4g087920 38.415 164 96 4 70 230 69 230 1.48e-21 90.9
MS.gene054850.t1 MTR_2g015890 33.333 138 83 3 109 241 2 135 1.71e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054850.t1 AT2G41130 61.187 219 79 5 18 233 10 225 1.96e-79 239
MS.gene054850.t1 AT3G56770 61.140 193 61 4 70 250 39 229 1.80e-72 221
MS.gene054850.t1 AT1G68810 45.729 199 96 3 57 243 151 349 1.06e-45 157
MS.gene054850.t1 AT3G25710 58.571 140 56 1 72 211 128 265 8.74e-44 151
MS.gene054850.t1 AT2G40200 41.579 190 94 4 71 245 58 245 1.83e-34 124
MS.gene054850.t1 AT4G38070 34.254 181 103 5 26 206 50 214 2.09e-21 90.5

Find 47 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACTGTTATTTCGTCGGTTT+CGG 0.235930 7.3:+20158913 None:intergenic
TTTCGATTAGGTCGGGAATT+AGG 0.256589 7.3:+20158813 None:intergenic
CAACAGATTCAGAATAATAT+TGG 0.265015 7.3:-20160270 MS.gene054850:CDS
GTAATAGTTATTGTTGTTAT+CGG 0.378138 7.3:+20160137 None:intergenic
GAATTCACTCAGGTCTTTGT+TGG 0.378871 7.3:-20158663 MS.gene054850:CDS
GAGACTTGAGGATTTCGATT+AGG 0.380801 7.3:+20158801 None:intergenic
AACAGATTCAGAATAATATT+GGG 0.426141 7.3:-20160269 MS.gene054850:CDS
TGTTGCATCATAGTGCTACT+AGG 0.431842 7.3:+20160177 None:intergenic
GCTGAAATGGCCACACTTGG+TGG 0.453567 7.3:-20158758 MS.gene054850:CDS
GTCGAAAAGGCGTCGAGGGT+TGG 0.453710 7.3:-20158615 MS.gene054850:CDS
GTTGAGTATTCAAAGAATGA+AGG 0.456323 7.3:+20160201 None:intergenic
CACTCAGGTCTTTGTTGGAT+AGG 0.462227 7.3:-20158658 MS.gene054850:CDS
AGGAAGCTGAGAAGAGAAGA+AGG 0.471315 7.3:-20160038 MS.gene054850:CDS
GGCAATGACATCTCCGACGA+AGG 0.483297 7.3:-20158881 MS.gene054850:CDS
GTTATTTCGTCGGTTTCGGA+TGG 0.483862 7.3:+20158917 None:intergenic
GTAATTTCAGAAACTTCAAA+GGG 0.483898 7.3:+20160111 None:intergenic
TTGAGGATTTCGATTAGGTC+GGG 0.493776 7.3:+20158806 None:intergenic
AGTAATTTCAGAAACTTCAA+AGG 0.496460 7.3:+20160110 None:intergenic
CTTGAGGATTTCGATTAGGT+CGG 0.504746 7.3:+20158805 None:intergenic
CCTTGGAGTTGCAAGGGAGA+AGG 0.512266 7.3:+20159980 None:intergenic
CTTGGAGTTGCAAGGGAGAA+GGG 0.521866 7.3:+20159981 None:intergenic
TTCGTCGGAGATGTCATTGC+CGG 0.522101 7.3:+20158883 None:intergenic
TTGAGTATTCAAAGAATGAA+GGG 0.546799 7.3:+20160202 None:intergenic
AAAGCTGAAATGGCCACACT+TGG 0.548268 7.3:-20158761 MS.gene054850:CDS
CCTTCTCCCTTGCAACTCCA+AGG 0.549041 7.3:-20159980 MS.gene054850:intron
ATAATAACCTTGGAGTTGCA+AGG 0.549750 7.3:+20159973 None:intergenic
TCTTTGTTGGATAGGTCTAG+TGG 0.556474 7.3:-20158650 MS.gene054850:CDS
AGATATGACTGTTATTTCGT+CGG 0.558305 7.3:+20158907 None:intergenic
GGTCTAGTGGTTGTAACGAT+AGG 0.565435 7.3:-20158637 MS.gene054850:CDS
TAATAACCTTGGAGTTGCAA+GGG 0.573577 7.3:+20159974 None:intergenic
AAGGGAGAAGGGTACGAAGA+TGG 0.575464 7.3:+20159992 None:intergenic
ACGTGTTCTTCCACCAAGTG+TGG 0.575747 7.3:+20158748 None:intergenic
GAATATGAGTCTGCCTTCGT+CGG 0.576237 7.3:+20158868 None:intergenic
ATAGGTCGAAAAGGCGTCGA+GGG 0.576982 7.3:-20158619 MS.gene054850:CDS
AGAGCTCTATCTTGCTGAGA+GGG 0.579252 7.3:+20160081 None:intergenic
GTTGTAACGATAGGTCGAAA+AGG 0.580273 7.3:-20158628 MS.gene054850:CDS
TTTCAGAAACTTCAAAGGGG+TGG 0.587837 7.3:+20160115 None:intergenic
GAAATAACAGTCATATCTGC+CGG 0.595239 7.3:-20158902 MS.gene054850:CDS
GATTCAGAATAATATTGGGA+TGG 0.603750 7.3:-20160265 MS.gene054850:CDS
GGAAGCTGAGAAGAGAAGAA+GGG 0.604105 7.3:-20160037 MS.gene054850:CDS
GAAGGGTACGAAGATGGTCA+AGG 0.633890 7.3:+20159998 None:intergenic
AGCTGCTTTGAAGAATCACA+AGG 0.645458 7.3:-20160058 MS.gene054850:CDS
TAGAGCTCTATCTTGCTGAG+AGG 0.647696 7.3:+20160080 None:intergenic
ATGAAGGGTTGTTAGCAACA+AGG 0.651347 7.3:+20160217 None:intergenic
GATAGGTCGAAAAGGCGTCG+AGG 0.658588 7.3:-20158620 MS.gene054850:CDS
TGATTGATTTCAGACAGACA+AGG 0.674472 7.3:-20159020 MS.gene054850:intron
TAATTTCAGAAACTTCAAAG+GGG 0.757536 7.3:+20160112 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTTTTTACCATTTA+AGG + chr7.3:20159454-20159473 None:intergenic 10.0%
!! AACAGATTCAGAATAATATT+GGG - chr7.3:20158605-20158624 MS.gene054850:CDS 20.0%
!! AATGATGAAAATAATAACCT+TGG + chr7.3:20158914-20158933 None:intergenic 20.0%
!! AATTATTTGCAAATTAAGCT+AGG - chr7.3:20159478-20159497 MS.gene054850:intron 20.0%
!! ATTATTTGCAAATTAAGCTA+GGG - chr7.3:20159479-20159498 MS.gene054850:intron 20.0%
!! GATGTTCCAAAAAAAAATTA+AGG + chr7.3:20159262-20159281 None:intergenic 20.0%
!! TCATTCTTAATTATTTGTCT+TGG - chr7.3:20158929-20158948 MS.gene054850:CDS 20.0%
!!! CTAGATCCTTAATTTTTTTT+TGG - chr7.3:20159253-20159272 MS.gene054850:intron 20.0%
!!! GCTTAATTTGCAAATAATTT+TGG + chr7.3:20159477-20159496 None:intergenic 20.0%
!!! GTAATAGTTATTGTTGTTAT+CGG + chr7.3:20158740-20158759 None:intergenic 20.0%
!!! TTGGATTACATATTTTTTGT+AGG + chr7.3:20159411-20159430 None:intergenic 20.0%
! AATCACTCAAAATCCTTAAA+TGG - chr7.3:20159438-20159457 MS.gene054850:intron 25.0%
! AGTAATTTCAGAAACTTCAA+AGG + chr7.3:20158767-20158786 None:intergenic 25.0%
! CAACAGATTCAGAATAATAT+TGG - chr7.3:20158604-20158623 MS.gene054850:CDS 25.0%
! CTTAATTACTAAAGTGAGAA+AGG + chr7.3:20159152-20159171 None:intergenic 25.0%
! GTAATTTCAGAAACTTCAAA+GGG + chr7.3:20158766-20158785 None:intergenic 25.0%
! TAATTTCAGAAACTTCAAAG+GGG + chr7.3:20158765-20158784 None:intergenic 25.0%
! TAGTCATGTATAAAACTTGA+TGG + chr7.3:20159568-20159587 None:intergenic 25.0%
! TTGAGTATTCAAAGAATGAA+GGG + chr7.3:20158675-20158694 None:intergenic 25.0%
!! ACATGTTTTCACTCTTTTTT+TGG + chr7.3:20159097-20159116 None:intergenic 25.0%
!! TCAATTTTACAAAACCTACA+AGG - chr7.3:20159686-20159705 MS.gene054850:intron 25.0%
!! TTTTGTTCTTTTGTTCTCAA+AGG - chr7.3:20158995-20159014 MS.gene054850:CDS 25.0%
!!! TTGTAGGTTTTGTAAAATTG+AGG + chr7.3:20159687-20159706 None:intergenic 25.0%
!!! TTTTGAGTGATTAAGTTCAT+TGG + chr7.3:20159430-20159449 None:intergenic 25.0%
AAAAACTCTCAAAGCTGAAA+TGG - chr7.3:20160103-20160122 MS.gene054850:CDS 30.0%
AAACCATTGTGATTTAGAAG+AGG + chr7.3:20159714-20159733 None:intergenic 30.0%
GATTCAGAATAATATTGGGA+TGG - chr7.3:20158609-20158628 MS.gene054850:CDS 30.0%
GTTGAGTATTCAAAGAATGA+AGG + chr7.3:20158676-20158695 None:intergenic 30.0%
! ATTTTCTTCAGAATTCACTC+AGG - chr7.3:20160201-20160220 MS.gene054850:CDS 30.0%
! CTTTAGTAATTAAGTTGCCA+TGG - chr7.3:20159158-20159177 MS.gene054850:intron 30.0%
!! AGATATGACTGTTATTTCGT+CGG + chr7.3:20159970-20159989 None:intergenic 30.0%
!!! GTTTTCACTCTTTTTTTGGA+TGG + chr7.3:20159093-20159112 None:intergenic 30.0%
!!! TTTCAGCTTTGAGAGTTTTT+AGG + chr7.3:20160103-20160122 None:intergenic 30.0%
ACACACACAATTATCTTTGC+TGG + chr7.3:20159036-20159055 None:intergenic 35.0%
ATAATAACCTTGGAGTTGCA+AGG + chr7.3:20158904-20158923 None:intergenic 35.0%
CTAGTACATGTTTGCATATG+TGG + chr7.3:20159749-20159768 None:intergenic 35.0%
GAAATAACAGTCATATCTGC+CGG - chr7.3:20159972-20159991 MS.gene054850:intron 35.0%
TAATAACCTTGGAGTTGCAA+GGG + chr7.3:20158903-20158922 None:intergenic 35.0%
TCAATCTTGTCACAAAACCT+AGG + chr7.3:20159791-20159810 None:intergenic 35.0%
TGATGAAGAAAGCAAAACCT+AGG - chr7.3:20159771-20159790 MS.gene054850:intron 35.0%
! AGGCATATTTTCACTCATTC+TGG + chr7.3:20159132-20159151 None:intergenic 35.0%
! AGTATCGAACTCAAAACATC+CGG + chr7.3:20159386-20159405 None:intergenic 35.0%
! GTAAAGTACTACATGTTCCA+TGG + chr7.3:20159178-20159197 None:intergenic 35.0%
! TGATTGATTTCAGACAGACA+AGG - chr7.3:20159854-20159873 MS.gene054850:intron 35.0%
!!! TTTTTAGGTGAAGAGACTTG+AGG + chr7.3:20160088-20160107 None:intergenic 35.0%
ATGAAGGGTTGTTAGCAACA+AGG + chr7.3:20158660-20158679 None:intergenic 40.0%
ATTTAGAAGAGGCTCCTTGT+AGG + chr7.3:20159703-20159722 None:intergenic 40.0%
CTTGAGGATTTCGATTAGGT+CGG + chr7.3:20160072-20160091 None:intergenic 40.0%
GAATTCACTCAGGTCTTTGT+TGG - chr7.3:20160211-20160230 MS.gene054850:CDS 40.0%
GAGACTTGAGGATTTCGATT+AGG + chr7.3:20160076-20160095 None:intergenic 40.0%
GAGCCTCTTCTAAATCACAA+TGG - chr7.3:20159708-20159727 MS.gene054850:intron 40.0%
GTTGTAACGATAGGTCGAAA+AGG - chr7.3:20160246-20160265 MS.gene054850:CDS 40.0%
TCTTATGCGTTAAGCACAAG+TGG - chr7.3:20159326-20159345 MS.gene054850:intron 40.0%
TCTTTGTTGGATAGGTCTAG+TGG - chr7.3:20160224-20160243 MS.gene054850:CDS 40.0%
TTAAGCTAGGGAAGTCACTT+AGG - chr7.3:20159491-20159510 MS.gene054850:intron 40.0%
TTGAGGATTTCGATTAGGTC+GGG + chr7.3:20160071-20160090 None:intergenic 40.0%
TTTCAGAAACTTCAAAGGGG+TGG + chr7.3:20158762-20158781 None:intergenic 40.0%
TTTCGATTAGGTCGGGAATT+AGG + chr7.3:20160064-20160083 None:intergenic 40.0%
! AGGGAAGTCACTTAGGTTTT+AGG - chr7.3:20159498-20159517 MS.gene054850:intron 40.0%
! TAGCTTTTGCAAAATCGTGG+TGG + chr7.3:20159659-20159678 None:intergenic 40.0%
! TCGTAGCTTTTGCAAAATCG+TGG + chr7.3:20159662-20159681 None:intergenic 40.0%
! TGTCTGAAGTGGTGTATATC+CGG - chr7.3:20159364-20159383 MS.gene054850:intron 40.0%
! TGTTGCATCATAGTGCTACT+AGG + chr7.3:20158700-20158719 None:intergenic 40.0%
!! AGCTGCTTTGAAGAATCACA+AGG - chr7.3:20158816-20158835 MS.gene054850:CDS 40.0%
!! GACTGTTATTTCGTCGGTTT+CGG + chr7.3:20159964-20159983 None:intergenic 40.0%
AAAGCTGAAATGGCCACACT+TGG - chr7.3:20160113-20160132 MS.gene054850:CDS 45.0%
AGAGCTCTATCTTGCTGAGA+GGG + chr7.3:20158796-20158815 None:intergenic 45.0%
AGGAAGCTGAGAAGAGAAGA+AGG - chr7.3:20158836-20158855 MS.gene054850:CDS 45.0%
ATGCGTTAAGCACAAGTGGT+CGG - chr7.3:20159330-20159349 MS.gene054850:intron 45.0%
CACTCAGGTCTTTGTTGGAT+AGG - chr7.3:20160216-20160235 MS.gene054850:CDS 45.0%
GAATATGAGTCTGCCTTCGT+CGG + chr7.3:20160009-20160028 None:intergenic 45.0%
GGAAGCTGAGAAGAGAAGAA+GGG - chr7.3:20158837-20158856 MS.gene054850:CDS 45.0%
GGTCTAGTGGTTGTAACGAT+AGG - chr7.3:20160237-20160256 MS.gene054850:CDS 45.0%
TAGAGCTCTATCTTGCTGAG+AGG + chr7.3:20158797-20158816 None:intergenic 45.0%
TGCGTAATGTGTGTCTGAAG+TGG - chr7.3:20159353-20159372 MS.gene054850:intron 45.0%
!! GTTATTTCGTCGGTTTCGGA+TGG + chr7.3:20159960-20159979 None:intergenic 45.0%
ACGTGTTCTTCCACCAAGTG+TGG + chr7.3:20160129-20160148 None:intergenic 50.0%
CTTGGAGTTGCAAGGGAGAA+GGG + chr7.3:20158896-20158915 None:intergenic 50.0%
TTCGTCGGAGATGTCATTGC+CGG + chr7.3:20159994-20160013 None:intergenic 50.0%
! AAGGGAGAAGGGTACGAAGA+TGG + chr7.3:20158885-20158904 None:intergenic 50.0%
! GAAGGGTACGAAGATGGTCA+AGG + chr7.3:20158879-20158898 None:intergenic 50.0%
!! ATAGGTCGAAAAGGCGTCGA+GGG - chr7.3:20160255-20160274 MS.gene054850:CDS 50.0%
CCTTCTCCCTTGCAACTCCA+AGG - chr7.3:20158894-20158913 MS.gene054850:CDS 55.0%
CCTTGGAGTTGCAAGGGAGA+AGG + chr7.3:20158897-20158916 None:intergenic 55.0%
GCTGAAATGGCCACACTTGG+TGG - chr7.3:20160116-20160135 MS.gene054850:CDS 55.0%
GGCAATGACATCTCCGACGA+AGG - chr7.3:20159993-20160012 MS.gene054850:CDS 55.0%
!! GATAGGTCGAAAAGGCGTCG+AGG - chr7.3:20160254-20160273 MS.gene054850:CDS 55.0%
!! GTCGAAAAGGCGTCGAGGGT+TGG - chr7.3:20160259-20160278 MS.gene054850:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 20158595 20160301 20158595 ID=MS.gene054850
chr7.3 mRNA 20158595 20160301 20158595 ID=MS.gene054850.t1;Parent=MS.gene054850
chr7.3 exon 20159981 20160301 20159981 ID=MS.gene054850.t1.exon1;Parent=MS.gene054850.t1
chr7.3 CDS 20159981 20160301 20159981 ID=cds.MS.gene054850.t1;Parent=MS.gene054850.t1
chr7.3 exon 20158595 20159029 20158595 ID=MS.gene054850.t1.exon2;Parent=MS.gene054850.t1
chr7.3 CDS 20158595 20159029 20158595 ID=cds.MS.gene054850.t1;Parent=MS.gene054850.t1
Gene Sequence

>MS.gene054850

ATGCTTCAGCAACAGATTCAGAATAATATTGGGATGGAAAACAACTCTGAACTCTTTCAATTCCTTGTTGCTAACAACCCTTCATTCTTTGAATACTCAACACCTAGTAGCACTATGATGCAACAAAGTTTCTGCAGTTCTTCCGATAACAACAATAACTATTACCACCCCTTTGAAGTTTCTGAAATTACTGATACACCCTCTCAGCAAGATAGAGCTCTAGCTGCTTTGAAGAATCACAAGGAAGCTGAGAAGAGAAGAAGGGAAAGAATTAACTCTCACCTTGACCATCTTCGTACCCTTCTCCCTTGCAACTCCAAGGTTATTATTTTCATCATTCTTAATTATTTGTCTTGGACAAATTATCTTAAAGCATATTTCTTTTTCTTTAGATTTTCTTTTTTGTTCTTTTGTTCTCAAAGGTTAACTACTTTTTGACCAGCAAAGATAATTGTGTGTGTTTTAGTTGACTAAATAATTATGATTTACTTCATTCCATCCAAAAAAAGAGTGAAAACATGTAGTACTTTTCTACCAGAATGAGTGAAAATATGCCTTTCTCACTTTAGTAATTAAGTTGCCATGGAACATGTAGTACTTTACAATAGTAGAATGAGTGAAAATGAAAAAGAAAATAGTAAATAAAATTAAAGAAACTCTAGATCCTTAATTTTTTTTTGGAACATCAACAAAATTGAAAGAAATATTAGCTGATGTATTTATAAAAAAAATCTTATGCGTTAAGCACAAGTGGTCGGTGCGTAATGTGTGTCTGAAGTGGTGTATATCCGGATGTTTTGAGTTCGATACTTACCTACAAAAAATATGTAATCCAATGAACTTAATCACTCAAAATCCTTAAATGGTAAAAAAATAAAACCAAAATTATTTGCAAATTAAGCTAGGGAAGTCACTTAGGTTTTAGGAGACTACTTTCTTCACTCAAACATCACTTTTGCATAAAAAATAACCATCAAGTTTTATACATGACTATACATATTTTCTAGATAATTTGAGTTGTTAAGATGAGATTCATGACGAGTCTCTCAGAATTACATTCACCACCACGATTTTGCAAAAGCTACGACACCTCAATTTTACAAAACCTACAAGGAGCCTCTTCTAAATCACAATGGTTTACTTTGAACTCACCACATATGCAAACATGTACTAGAATGATGAAGAAAGCAAAACCTAGGTTTTGTGACAAGATTGATTCTACATGTTAATAGTTAATTCTTTATTTTAATATTTGCGTTTGATTGATTTCAGACAGACAAGGCTTCACTTCTAGCAAAAGTAGTAGAACGTGTGAAAGAATTAAAGCAACAAACATCTCAAATCACACAACTCGAAACTGTTCCATCCGAAACCGACGAAATAACAGTCATATCTGCCGGCAATGACATCTCCGACGAAGGCAGACTCATATTCAAAGCTTCATTATGCTGCGAAGATCGCTTCGACCTAATTCCCGACCTAATCGAAATCCTCAAGTCTCTTCACCTAAAAACTCTCAAAGCTGAAATGGCCACACTTGGTGGAAGAACACGTAACGTTCTTGTTGTTGCTGCTGAGAAAGAACATAATAGCATTGAATCTATTCATTTTCTTCAGAATTCACTCAGGTCTTTGTTGGATAGGTCTAGTGGTTGTAACGATAGGTCGAAAAGGCGTCGAGGGTTGGATAGAAGAATGATGCCTTAA

Protein sequence

>MS.gene054850.t1

MLQQQIQNNIGMENNSELFQFLVANNPSFFEYSTPSSTMMQQSFCSSSDNNNNYYHPFEVSEITDTPSQQDRALAALKNHKEAEKRRRERINSHLDHLRTLLPCNSKTDKASLLAKVVERVKELKQQTSQITQLETVPSETDEITVISAGNDISDEGRLIFKASLCCEDRFDLIPDLIEILKSLHLKTLKAEMATLGGRTRNVLVVAAEKEHNSIESIHFLQNSLRSLLDRSSGCNDRSKRRRGLDRRMMP