AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054941


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054941.t1 MTR_3g095980 98.876 178 2 0 1 178 1 178 9.36e-130 361
MS.gene054941.t1 MTR_4g122740 29.310 174 114 4 5 177 13 178 8.76e-17 74.3
MS.gene054941.t1 MTR_2g033630 32.335 167 106 5 12 176 15 176 5.18e-16 72.0
MS.gene054941.t1 MTR_4g122890 30.726 179 105 6 7 176 7 175 7.86e-15 68.9
MS.gene054941.t1 MTR_8g009300 26.012 173 116 6 13 177 13 181 4.72e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054941.t1 AT5G64620 50.000 180 87 1 1 177 1 180 3.03e-63 193
MS.gene054941.t1 AT3G17220 27.879 165 114 3 12 176 14 173 2.24e-13 65.1
MS.gene054941.t1 AT4G15750 29.197 137 95 1 9 145 8 142 5.01e-13 64.3
MS.gene054941.t1 AT5G46930 28.313 166 104 6 5 161 1 160 3.87e-11 59.3

Find 39 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACATAGATTGTAGTATT+TGG 0.248640 3.1:+76448423 None:intergenic
ATTCAAAAGGGTTCCTGATT+TGG 0.274182 3.1:-76448104 MS.gene054941:CDS
GAGCTTGCCACTAGAGAAAA+TGG 0.275669 3.1:-76448070 MS.gene054941:CDS
GCACACTATGCATTTGTAAA+TGG 0.303461 3.1:-76448481 MS.gene054941:CDS
CCAAGCTTCAGTACAAGATT+TGG 0.336276 3.1:-76448200 MS.gene054941:CDS
AGGCCCTTTGTGTCTGCGTT+TGG 0.336742 3.1:+76448373 None:intergenic
GGTTGGAGTAAGAGATTTAG+AGG 0.338526 3.1:+76448291 None:intergenic
CAACAACACTACCTTGAAAA+AGG 0.368143 3.1:-76448269 MS.gene054941:CDS
ACAAGCATTTGGGTAGTCTT+TGG 0.377171 3.1:+76448135 None:intergenic
ACTTATCTGCACACTCTTTG+AGG 0.396879 3.1:+76448245 None:intergenic
AATGCATTGTGACAAGCATT+TGG 0.398656 3.1:+76448124 None:intergenic
CTTGTCACAATGCATTCAAA+AGG 0.421269 3.1:-76448117 MS.gene054941:CDS
TCAGGAGGGTAAACCAAATC+AGG 0.429293 3.1:+76448091 None:intergenic
GCCTAGCTGTGATCATGGTT+GGG 0.442882 3.1:-76448354 MS.gene054941:CDS
TGCGTTTGGACTGCTAGGAT+TGG 0.446964 3.1:+76448387 None:intergenic
TTCAAGGTAGTGTTGTTGGT+TGG 0.453115 3.1:+76448274 None:intergenic
CCAAATCTTGTACTGAAGCT+TGG 0.454754 3.1:+76448200 None:intergenic
GTGTCTGCGTTTGGACTGCT+AGG 0.461087 3.1:+76448382 None:intergenic
GGCCTAGCTGTGATCATGGT+TGG 0.485514 3.1:-76448355 MS.gene054941:CDS
CATATATGTGATGTAGCTAT+GGG 0.497869 3.1:-76448040 MS.gene054941:CDS
ATGCATTGTGACAAGCATTT+GGG 0.500606 3.1:+76448125 None:intergenic
ACATATATGTGATGTAGCTA+TGG 0.505961 3.1:-76448041 MS.gene054941:CDS
GGATGAGAAATAGTGAGAAG+TGG 0.509606 3.1:+76448512 None:intergenic
TGATAATCTCATCAATATGT+AGG 0.512388 3.1:-76448011 None:intergenic
TTACAAATGCATAGTGTGCT+AGG 0.521367 3.1:+76448485 None:intergenic
AAAGGGCCTAGCTGTGATCA+TGG 0.521391 3.1:-76448359 MS.gene054941:CDS
GCTGTGATCATGGTTGGGAT+TGG 0.523662 3.1:-76448349 MS.gene054941:CDS
TCTCTAGTGGCAAGCTCAGG+AGG 0.523727 3.1:+76448076 None:intergenic
AATCTTGTACTGAAGCTTGG+AGG 0.528434 3.1:+76448203 None:intergenic
ATGCATAGTGTGCTAGGAAG+AGG 0.536460 3.1:+76448491 None:intergenic
AGTCCAAACGCAGACACAAA+GGG 0.542036 3.1:-76448376 MS.gene054941:CDS
ATCTTGTACTGAAGCTTGGA+GGG 0.545648 3.1:+76448204 None:intergenic
AGAGGACAAATATGAAGAAG+TGG 0.553621 3.1:+76448309 None:intergenic
TTGTCACAATGCATTCAAAA+GGG 0.564017 3.1:-76448116 MS.gene054941:CDS
CTCTAGTGGCAAGCTCAGGA+GGG 0.593107 3.1:+76448077 None:intergenic
CAGTCCAAACGCAGACACAA+AGG 0.620929 3.1:-76448377 MS.gene054941:CDS
TCCCAACCATGATCACAGCT+AGG 0.662886 3.1:+76448353 None:intergenic
GCAGATAAGTACAACTATGC+TGG 0.666996 3.1:-76448232 MS.gene054941:CDS
ATATATGTGATGTAGCTATG+GGG 0.736245 3.1:-76448039 MS.gene054941:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAAACATAGATTGTAGTATT+TGG + chr3.1:76448118-76448137 None:intergenic 25.0%
ACATATATGTGATGTAGCTA+TGG - chr3.1:76448497-76448516 MS.gene054941:CDS 30.0%
ATATATGTGATGTAGCTATG+GGG - chr3.1:76448499-76448518 MS.gene054941:CDS 30.0%
CATATATGTGATGTAGCTAT+GGG - chr3.1:76448498-76448517 MS.gene054941:CDS 30.0%
TTGTCACAATGCATTCAAAA+GGG - chr3.1:76448422-76448441 MS.gene054941:CDS 30.0%
! TTTCAAACCATTTTCTCTAG+TGG + chr3.1:76448478-76448497 None:intergenic 30.0%
AATGCATTGTGACAAGCATT+TGG + chr3.1:76448417-76448436 None:intergenic 35.0%
AGAGGACAAATATGAAGAAG+TGG + chr3.1:76448232-76448251 None:intergenic 35.0%
ATGCATTGTGACAAGCATTT+GGG + chr3.1:76448416-76448435 None:intergenic 35.0%
ATTCAAAAGGGTTCCTGATT+TGG - chr3.1:76448434-76448453 MS.gene054941:CDS 35.0%
CAACAACACTACCTTGAAAA+AGG - chr3.1:76448269-76448288 MS.gene054941:CDS 35.0%
CTTGTCACAATGCATTCAAA+AGG - chr3.1:76448421-76448440 MS.gene054941:CDS 35.0%
GCACACTATGCATTTGTAAA+TGG - chr3.1:76448057-76448076 MS.gene054941:CDS 35.0%
TTACAAATGCATAGTGTGCT+AGG + chr3.1:76448056-76448075 None:intergenic 35.0%
!!! CTTTTTCAAGGTAGTGTTGT+TGG + chr3.1:76448271-76448290 None:intergenic 35.0%
AATCTTGTACTGAAGCTTGG+AGG + chr3.1:76448338-76448357 None:intergenic 40.0%
ACAAGCATTTGGGTAGTCTT+TGG + chr3.1:76448406-76448425 None:intergenic 40.0%
ACTTATCTGCACACTCTTTG+AGG + chr3.1:76448296-76448315 None:intergenic 40.0%
ATCTTGTACTGAAGCTTGGA+GGG + chr3.1:76448337-76448356 None:intergenic 40.0%
CCAAATCTTGTACTGAAGCT+TGG + chr3.1:76448341-76448360 None:intergenic 40.0%
CCAAGCTTCAGTACAAGATT+TGG - chr3.1:76448338-76448357 MS.gene054941:CDS 40.0%
GCAGATAAGTACAACTATGC+TGG - chr3.1:76448306-76448325 MS.gene054941:CDS 40.0%
GGATGAGAAATAGTGAGAAG+TGG + chr3.1:76448029-76448048 None:intergenic 40.0%
GGTTGGAGTAAGAGATTTAG+AGG + chr3.1:76448250-76448269 None:intergenic 40.0%
! GCAAGTGCTTTTGATCAAAG+TGG + chr3.1:76448088-76448107 None:intergenic 40.0%
! TTCAAGGTAGTGTTGTTGGT+TGG + chr3.1:76448267-76448286 None:intergenic 40.0%
!! CTCTTTGAGGACCTTTTTCA+AGG + chr3.1:76448283-76448302 None:intergenic 40.0%
AGTCCAAACGCAGACACAAA+GGG - chr3.1:76448162-76448181 MS.gene054941:CDS 45.0%
ATGCATAGTGTGCTAGGAAG+AGG + chr3.1:76448050-76448069 None:intergenic 45.0%
GAGCTTGCCACTAGAGAAAA+TGG - chr3.1:76448468-76448487 MS.gene054941:CDS 45.0%
TCAGGAGGGTAAACCAAATC+AGG + chr3.1:76448450-76448469 None:intergenic 45.0%
! TTTTCTCTAGTGGCAAGCTC+AGG + chr3.1:76448468-76448487 None:intergenic 45.0%
AAAGGGCCTAGCTGTGATCA+TGG - chr3.1:76448179-76448198 MS.gene054941:CDS 50.0%
CAGTCCAAACGCAGACACAA+AGG - chr3.1:76448161-76448180 MS.gene054941:CDS 50.0%
GCCTAGCTGTGATCATGGTT+GGG - chr3.1:76448184-76448203 MS.gene054941:CDS 50.0%
GCTGTGATCATGGTTGGGAT+TGG - chr3.1:76448189-76448208 MS.gene054941:CDS 50.0%
TCCCAACCATGATCACAGCT+AGG + chr3.1:76448188-76448207 None:intergenic 50.0%
TGCGTTTGGACTGCTAGGAT+TGG + chr3.1:76448154-76448173 None:intergenic 50.0%
AGGCCCTTTGTGTCTGCGTT+TGG + chr3.1:76448168-76448187 None:intergenic 55.0%
CTCTAGTGGCAAGCTCAGGA+GGG + chr3.1:76448464-76448483 None:intergenic 55.0%
GGCCTAGCTGTGATCATGGT+TGG - chr3.1:76448183-76448202 MS.gene054941:CDS 55.0%
GTGTCTGCGTTTGGACTGCT+AGG + chr3.1:76448159-76448178 None:intergenic 55.0%
TCTCTAGTGGCAAGCTCAGG+AGG + chr3.1:76448465-76448484 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 76448012 76448548 76448012 ID=MS.gene054941
chr3.1 mRNA 76448012 76448548 76448012 ID=MS.gene054941.t1;Parent=MS.gene054941
chr3.1 exon 76448012 76448548 76448012 ID=MS.gene054941.t1.exon1;Parent=MS.gene054941.t1
chr3.1 CDS 76448012 76448548 76448012 ID=cds.MS.gene054941.t1;Parent=MS.gene054941.t1
Gene Sequence

>MS.gene054941

ATGGCTTCTAAGATCCACTTCTCACTATTTCTCATCCTCTTCCTAGCACACTATGCATTTGTAAATGGAGATGCCACTTTGATCAAAAGCACTTGCAAGAACACCAAATACTACAATCTATGTTTCTCTTCTCTCAAATCCAATCCTAGCAGTCCAAACGCAGACACAAAGGGCCTAGCTGTGATCATGGTTGGGATTGGAATGACAAATGCTACTTCCACTTCTTCATATTTGTCCTCTAAATCTCTTACTCCAACCAACAACACTACCTTGAAAAAGGTCCTCAAAGAGTGTGCAGATAAGTACAACTATGCTGGTGATTCCCTCCAAGCTTCAGTACAAGATTTGGCTAATGAAGAATATGATTATGCTTACATACACATCACTGCAGCCAAAGACTACCCAAATGCTTGTCACAATGCATTCAAAAGGGTTCCTGATTTGGTTTACCCTCCTGAGCTTGCCACTAGAGAAAATGGTTTGAAACATATATGTGATGTAGCTATGGGGATCATTGATAATCTCATCAATATGTAG

Protein sequence

>MS.gene054941.t1

MASKIHFSLFLILFLAHYAFVNGDATLIKSTCKNTKYYNLCFSSLKSNPSSPNADTKGLAVIMVGIGMTNATSTSSYLSSKSLTPTNNTTLKKVLKECADKYNYAGDSLQASVQDLANEEYDYAYIHITAAKDYPNACHNAFKRVPDLVYPPELATRENGLKHICDVAMGIIDNLINM