Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049298.t1 | XP_003602589.1 | 98.3 | 178 | 3 | 0 | 1 | 178 | 1 | 178 | 1.30E-92 | 349 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049298.t1 | O49603 | 50.3 | 179 | 86 | 1 | 1 | 176 | 1 | 179 | 5.7e-44 | 178.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049298.t1 | G7JBI0 | 98.3 | 178 | 3 | 0 | 1 | 178 | 1 | 178 | 9.5e-93 | 349.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049298 | MS.gene051539 | 0.805678 | 1.19E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene051914 | 0.800551 | 1.37E-48 | -1.69E-46 |
| MS.gene049298 | MS.gene052169 | 0.812871 | 3.41E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene052209 | 0.843826 | 1.10E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene052308 | 0.819648 | 1.04E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene054662 | 0.80419 | 2.43E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene054850 | 0.809329 | 2.00E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene054941 | 0.947716 | 3.91E-106 | -1.69E-46 |
| MS.gene049298 | MS.gene056387 | -0.821336 | 4.26E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene05652 | 0.862443 | 5.05E-64 | -1.69E-46 |
| MS.gene049298 | MS.gene056618 | 0.834915 | 2.27E-56 | -1.69E-46 |
| MS.gene049298 | MS.gene057356 | 0.818225 | 2.19E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene057367 | 0.817829 | 2.69E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene057669 | 0.872309 | 3.51E-67 | -1.69E-46 |
| MS.gene049298 | MS.gene057717 | 0.83815 | 3.41E-57 | -1.69E-46 |
| MS.gene049298 | MS.gene058128 | 0.863479 | 2.42E-64 | -1.69E-46 |
| MS.gene049298 | MS.gene058853 | 0.859209 | 4.84E-63 | -1.69E-46 |
| MS.gene049298 | MS.gene05926 | 0.846565 | 2.01E-59 | -1.69E-46 |
| MS.gene049298 | MS.gene05960 | 0.804067 | 2.58E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene059616 | 0.84321 | 1.61E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene061357 | 0.809635 | 1.72E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene063035 | 0.808125 | 3.61E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene063235 | 0.827142 | 1.84E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene063279 | 0.841608 | 4.29E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene063280 | 0.845082 | 5.07E-59 | -1.69E-46 |
| MS.gene049298 | MS.gene063281 | 0.816446 | 5.51E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene063734 | 0.811749 | 5.99E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene06421 | 0.946963 | 1.69E-105 | -1.69E-46 |
| MS.gene049298 | MS.gene064328 | 0.854281 | 1.37E-61 | -1.69E-46 |
| MS.gene049298 | MS.gene064509 | 0.808358 | 3.22E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene065314 | 0.810445 | 1.15E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene065682 | 0.80025 | 1.57E-48 | -1.69E-46 |
| MS.gene049298 | MS.gene066026 | 0.82159 | 3.72E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene066265 | 0.816062 | 6.71E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene070268 | 0.802517 | 5.40E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene070324 | 0.851858 | 6.75E-61 | -1.69E-46 |
| MS.gene049298 | MS.gene070800 | 0.80337 | 3.60E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene071403 | 0.804695 | 1.91E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene071405 | 0.830363 | 3.06E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene071422 | 0.824851 | 6.45E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene071713 | 0.818263 | 2.15E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene072255 | 0.808059 | 3.73E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene072366 | 0.818016 | 2.44E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene073059 | 0.807166 | 5.77E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene073062 | 0.80997 | 1.45E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene074201 | 0.839272 | 1.75E-57 | -1.69E-46 |
| MS.gene049298 | MS.gene074380 | -0.812277 | 4.60E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene074515 | 0.874794 | 5.11E-68 | -1.69E-46 |
| MS.gene049298 | MS.gene08362 | 0.807423 | 5.09E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene21734 | 0.854688 | 1.04E-61 | -1.69E-46 |
| MS.gene049298 | MS.gene21888 | 0.85852 | 7.79E-63 | -1.69E-46 |
| MS.gene049298 | MS.gene22759 | 0.800247 | 1.58E-48 | -1.69E-46 |
| MS.gene049298 | MS.gene23050 | 0.800493 | 1.40E-48 | -1.69E-46 |
| MS.gene049298 | MS.gene23416 | 0.828202 | 1.02E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene25726 | 0.851796 | 7.03E-61 | -1.69E-46 |
| MS.gene049298 | MS.gene26122 | 0.800367 | 1.49E-48 | -1.69E-46 |
| MS.gene049298 | MS.gene26406 | 0.811597 | 6.47E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene26681 | 0.850905 | 1.25E-60 | -1.69E-46 |
| MS.gene049298 | MS.gene27560 | 0.853554 | 2.21E-61 | -1.69E-46 |
| MS.gene049298 | MS.gene27771 | 0.84779 | 9.25E-60 | -1.69E-46 |
| MS.gene049298 | MS.gene27811 | 0.818857 | 1.57E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene27812 | 0.814778 | 1.30E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene28158 | -0.823108 | 1.65E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene28334 | -0.827901 | 1.21E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene28494 | -0.802453 | 5.57E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene28923 | 0.829311 | 5.53E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene29215 | 0.840262 | 9.67E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene29939 | 0.829724 | 4.39E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene29940 | 0.82916 | 6.01E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene29942 | 0.829816 | 4.16E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene30743 | -0.803495 | 3.39E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene31917 | 0.818927 | 1.52E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene31973 | 0.836755 | 7.77E-57 | -1.69E-46 |
| MS.gene049298 | MS.gene33809 | 0.822029 | 2.95E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene34503 | 0.818505 | 1.89E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene34573 | 0.810103 | 1.36E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene34976 | 0.811826 | 5.76E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene35777 | 0.843471 | 1.37E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene35794 | 0.855229 | 7.26E-62 | -1.69E-46 |
| MS.gene049298 | MS.gene35795 | 0.877124 | 8.08E-69 | -1.69E-46 |
| MS.gene049298 | MS.gene35853 | 0.869843 | 2.29E-66 | -1.69E-46 |
| MS.gene049298 | MS.gene35931 | 0.876794 | 1.05E-68 | -1.69E-46 |
| MS.gene049298 | MS.gene36778 | 0.845256 | 4.55E-59 | -1.69E-46 |
| MS.gene049298 | MS.gene36820 | 0.847226 | 1.32E-59 | -1.69E-46 |
| MS.gene049298 | MS.gene36957 | 0.841698 | 4.06E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene36961 | 0.830632 | 2.63E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene38102 | 0.849283 | 3.57E-60 | -1.69E-46 |
| MS.gene049298 | MS.gene38166 | 0.852095 | 5.78E-61 | -1.69E-46 |
| MS.gene049298 | MS.gene39469 | 0.831097 | 2.02E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene39911 | 0.833323 | 5.70E-56 | -1.69E-46 |
| MS.gene049298 | MS.gene40241 | 0.827395 | 1.60E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene40630 | 0.823965 | 1.04E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene40689 | 0.814662 | 1.37E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene41190 | 0.853005 | 3.18E-61 | -1.69E-46 |
| MS.gene049298 | MS.gene43727 | 0.830772 | 2.43E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene43975 | 0.816028 | 6.83E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene45445 | 0.82307 | 1.69E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene46880 | -0.82379 | 1.15E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene49165 | 0.829797 | 4.21E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene49349 | 0.84088 | 6.66E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene49425 | 0.827003 | 1.99E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene50576 | 0.820526 | 6.55E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene51554 | 0.839041 | 2.01E-57 | -1.69E-46 |
| MS.gene049298 | MS.gene52072 | 0.834086 | 3.67E-56 | -1.69E-46 |
| MS.gene049298 | MS.gene52084 | 0.825913 | 3.62E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene52113 | 0.872017 | 4.39E-67 | -1.69E-46 |
| MS.gene049298 | MS.gene52363 | 0.808487 | 3.02E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene52403 | 0.824745 | 6.83E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene52980 | 0.83454 | 2.83E-56 | -1.69E-46 |
| MS.gene049298 | MS.gene53366 | 0.816922 | 4.31E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene53408 | 0.82055 | 6.47E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene54855 | 0.811771 | 5.93E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene58849 | 0.821939 | 3.09E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene60041 | 0.819083 | 1.40E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene60269 | 0.835036 | 2.12E-56 | -1.69E-46 |
| MS.gene049298 | MS.gene60853 | 0.800423 | 1.45E-48 | -1.69E-46 |
| MS.gene049298 | MS.gene61094 | 0.843529 | 1.32E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene61684 | 0.841269 | 5.26E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene61745 | 0.829637 | 4.61E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene62884 | 0.817992 | 2.47E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene63011 | 0.801994 | 6.92E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene64726 | 0.819464 | 1.15E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene65431 | 0.804082 | 2.56E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene66043 | 0.813416 | 2.59E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene66273 | 0.807395 | 5.16E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene68832 | 0.842453 | 2.56E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene69607 | 0.85809 | 1.05E-62 | -1.69E-46 |
| MS.gene049298 | MS.gene69722 | 0.808004 | 3.83E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene70491 | 0.846749 | 1.79E-59 | -1.69E-46 |
| MS.gene049298 | MS.gene71261 | 0.822074 | 2.88E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene72164 | 0.828719 | 7.69E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene72916 | -0.824974 | 6.03E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene72923 | 0.802831 | 4.65E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene73893 | 0.855943 | 4.49E-62 | -1.69E-46 |
| MS.gene049298 | MS.gene74548 | 0.819188 | 1.32E-52 | -1.69E-46 |
| MS.gene049298 | MS.gene75179 | 0.871192 | 8.24E-67 | -1.69E-46 |
| MS.gene049298 | MS.gene75365 | 0.833311 | 5.74E-56 | -1.69E-46 |
| MS.gene049298 | MS.gene75704 | 0.838566 | 2.67E-57 | -1.69E-46 |
| MS.gene049298 | MS.gene75761 | 0.806077 | 9.80E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene75915 | 0.805889 | 1.07E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene75918 | 0.813343 | 2.69E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene77500 | 0.828786 | 7.41E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene77503 | 0.827644 | 1.40E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene77904 | 0.803755 | 3.00E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene78560 | 0.80139 | 9.21E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene78624 | 0.812798 | 3.54E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene79461 | 0.843959 | 1.02E-58 | -1.69E-46 |
| MS.gene049298 | MS.gene79864 | 0.822037 | 2.93E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene80899 | 0.882478 | 1.01E-70 | -1.69E-46 |
| MS.gene049298 | MS.gene87345 | 0.806861 | 6.70E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene88616 | 0.831313 | 1.79E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene89090 | 0.8055 | 1.30E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene89653 | 0.806391 | 8.42E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene90842 | 0.876885 | 9.78E-69 | -1.69E-46 |
| MS.gene049298 | MS.gene91390 | 0.805889 | 1.07E-49 | -1.69E-46 |
| MS.gene049298 | MS.gene91871 | 0.807328 | 5.33E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene91968 | 0.83078 | 2.42E-55 | -1.69E-46 |
| MS.gene049298 | MS.gene92313 | 0.860414 | 2.10E-63 | -1.69E-46 |
| MS.gene049298 | MS.gene92996 | 0.821953 | 3.07E-53 | -1.69E-46 |
| MS.gene049298 | MS.gene93058 | 0.808773 | 2.63E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene93105 | 0.826587 | 2.50E-54 | -1.69E-46 |
| MS.gene049298 | MS.gene93762 | 0.848189 | 7.18E-60 | -1.69E-46 |
| MS.gene049298 | MS.gene94553 | 0.851379 | 9.22E-61 | -1.69E-46 |
| MS.gene049298 | MS.gene94954 | 0.877007 | 8.87E-69 | -1.69E-46 |
| MS.gene049298 | MS.gene95672 | 0.808412 | 3.14E-50 | -1.69E-46 |
| MS.gene049298 | MS.gene99885 | 0.841618 | 4.26E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049298.t1 | MTR_3g095980 | 98.315 | 178 | 3 | 0 | 1 | 178 | 1 | 178 | 4.07e-129 | 359 |
| MS.gene049298.t1 | MTR_4g122740 | 29.310 | 174 | 114 | 4 | 5 | 177 | 13 | 178 | 9.53e-17 | 74.3 |
| MS.gene049298.t1 | MTR_4g122890 | 32.778 | 180 | 100 | 7 | 7 | 176 | 7 | 175 | 1.35e-15 | 70.9 |
| MS.gene049298.t1 | MTR_2g033630 | 31.737 | 167 | 107 | 5 | 12 | 176 | 15 | 176 | 1.68e-15 | 70.9 |
| MS.gene049298.t1 | MTR_8g009300 | 26.012 | 173 | 116 | 6 | 13 | 177 | 13 | 181 | 4.77e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049298.t1 | AT5G64620 | 50.000 | 180 | 87 | 1 | 1 | 177 | 1 | 180 | 4.06e-63 | 192 |
| MS.gene049298.t1 | AT4G15750 | 29.787 | 141 | 95 | 1 | 5 | 145 | 6 | 142 | 1.10e-13 | 65.9 |
| MS.gene049298.t1 | AT3G17220 | 27.879 | 165 | 114 | 3 | 12 | 176 | 14 | 173 | 3.00e-13 | 64.7 |
Find 39 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAACATAGATTGTAGTATT+TGG | 0.248640 | 3.3:+79627463 | None:intergenic |
| ATTCAAAAGGGTTCCTGATT+TGG | 0.274182 | 3.3:-79627144 | MS.gene049298:CDS |
| GAGCTTGCCACTAGAGAAAA+TGG | 0.275669 | 3.3:-79627110 | MS.gene049298:CDS |
| CCAAGCTTCAGTACAAGATT+TGG | 0.336276 | 3.3:-79627240 | MS.gene049298:CDS |
| AGGCCCTTTGTGTCTGCGTT+TGG | 0.336742 | 3.3:+79627413 | None:intergenic |
| GGTTGGAGTAAGAGATTTAG+AGG | 0.338526 | 3.3:+79627331 | None:intergenic |
| CAACAACACTACCTTGAAAA+AGG | 0.368143 | 3.3:-79627309 | MS.gene049298:CDS |
| ACAAGCATTTGGGTAGTCTT+TGG | 0.377171 | 3.3:+79627175 | None:intergenic |
| ACTTATCTGCACACTCTTTG+AGG | 0.396879 | 3.3:+79627285 | None:intergenic |
| AATGCATTGTGACAAGCATT+TGG | 0.398656 | 3.3:+79627164 | None:intergenic |
| CTTGTCACAATGCATTCAAA+AGG | 0.421269 | 3.3:-79627157 | MS.gene049298:CDS |
| TCAGGAGGGTAAACCAAATC+AGG | 0.429293 | 3.3:+79627131 | None:intergenic |
| GCCTAGCTGTGATCATGGTT+GGG | 0.442882 | 3.3:-79627394 | MS.gene049298:CDS |
| TGCGTTTGGACTGCTAGGAT+TGG | 0.446964 | 3.3:+79627427 | None:intergenic |
| TTCAAGGTAGTGTTGTTGGT+TGG | 0.453115 | 3.3:+79627314 | None:intergenic |
| CCAAATCTTGTACTGAAGCT+TGG | 0.454754 | 3.3:+79627240 | None:intergenic |
| GTGTCTGCGTTTGGACTGCT+AGG | 0.461087 | 3.3:+79627422 | None:intergenic |
| GGCCTAGCTGTGATCATGGT+TGG | 0.485514 | 3.3:-79627395 | MS.gene049298:CDS |
| CATATATGTGATGTAGCTAT+GGG | 0.497869 | 3.3:-79627080 | MS.gene049298:CDS |
| ATGCATTGTGACAAGCATTT+GGG | 0.500606 | 3.3:+79627165 | None:intergenic |
| ACATATATGTGATGTAGCTA+TGG | 0.505961 | 3.3:-79627081 | MS.gene049298:CDS |
| TCACAAATGCATAGTGTGCT+AGG | 0.510583 | 3.3:+79627525 | None:intergenic |
| TGATAATCTCATCAATATGT+AGG | 0.512388 | 3.3:-79627051 | None:intergenic |
| AAAGGGCCTAGCTGTGATCA+TGG | 0.521391 | 3.3:-79627399 | MS.gene049298:CDS |
| GGAAGAGAAATAGTGAGAAG+TGG | 0.522237 | 3.3:+79627552 | None:intergenic |
| GCTGTGATCATGGTTGGGAT+TGG | 0.523662 | 3.3:-79627389 | MS.gene049298:CDS |
| TCTCTAGTGGCAAGCTCAGG+AGG | 0.523727 | 3.3:+79627116 | None:intergenic |
| AATCTTGTACTGAAGCTTGG+AGG | 0.528434 | 3.3:+79627243 | None:intergenic |
| GCACACTATGCATTTGTGAA+TGG | 0.533265 | 3.3:-79627521 | MS.gene049298:CDS |
| ATGCATAGTGTGCTAGGAAG+AGG | 0.536460 | 3.3:+79627531 | None:intergenic |
| AGTCCAAACGCAGACACAAA+GGG | 0.542036 | 3.3:-79627416 | MS.gene049298:CDS |
| ATCTTGTACTGAAGCTTGGA+GGG | 0.545648 | 3.3:+79627244 | None:intergenic |
| AGAGGACAAATATGAAGAAG+TGG | 0.553621 | 3.3:+79627349 | None:intergenic |
| TTGTCACAATGCATTCAAAA+GGG | 0.564017 | 3.3:-79627156 | MS.gene049298:CDS |
| CTCTAGTGGCAAGCTCAGGA+GGG | 0.593107 | 3.3:+79627117 | None:intergenic |
| CAGTCCAAACGCAGACACAA+AGG | 0.620929 | 3.3:-79627417 | MS.gene049298:CDS |
| TCCCAACCATGATCACAGCT+AGG | 0.662886 | 3.3:+79627393 | None:intergenic |
| GCAGATAAGTACAACTATGC+TGG | 0.666996 | 3.3:-79627272 | MS.gene049298:CDS |
| ATATATGTGATGTAGCTATG+GGG | 0.736245 | 3.3:-79627079 | MS.gene049298:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAACATAGATTGTAGTATT+TGG | + | chr3.3:79627158-79627177 | None:intergenic | 25.0% |
| ACATATATGTGATGTAGCTA+TGG | - | chr3.3:79627537-79627556 | MS.gene049298:CDS | 30.0% | |
| ATATATGTGATGTAGCTATG+GGG | - | chr3.3:79627539-79627558 | MS.gene049298:CDS | 30.0% | |
| CATATATGTGATGTAGCTAT+GGG | - | chr3.3:79627538-79627557 | MS.gene049298:CDS | 30.0% | |
| TTGTCACAATGCATTCAAAA+GGG | - | chr3.3:79627462-79627481 | MS.gene049298:CDS | 30.0% | |
| ! | TTTCAAACCATTTTCTCTAG+TGG | + | chr3.3:79627518-79627537 | None:intergenic | 30.0% |
| AATGCATTGTGACAAGCATT+TGG | + | chr3.3:79627457-79627476 | None:intergenic | 35.0% | |
| AGAGGACAAATATGAAGAAG+TGG | + | chr3.3:79627272-79627291 | None:intergenic | 35.0% | |
| ATGCATTGTGACAAGCATTT+GGG | + | chr3.3:79627456-79627475 | None:intergenic | 35.0% | |
| ATTCAAAAGGGTTCCTGATT+TGG | - | chr3.3:79627474-79627493 | MS.gene049298:CDS | 35.0% | |
| CAACAACACTACCTTGAAAA+AGG | - | chr3.3:79627309-79627328 | MS.gene049298:CDS | 35.0% | |
| CTTGTCACAATGCATTCAAA+AGG | - | chr3.3:79627461-79627480 | MS.gene049298:CDS | 35.0% | |
| !! | GCAAGTGCTTTTTATCAAAG+TGG | + | chr3.3:79627128-79627147 | None:intergenic | 35.0% |
| !!! | CTTTTTCAAGGTAGTGTTGT+TGG | + | chr3.3:79627311-79627330 | None:intergenic | 35.0% |
| AATCTTGTACTGAAGCTTGG+AGG | + | chr3.3:79627378-79627397 | None:intergenic | 40.0% | |
| ACAAGCATTTGGGTAGTCTT+TGG | + | chr3.3:79627446-79627465 | None:intergenic | 40.0% | |
| ACTTATCTGCACACTCTTTG+AGG | + | chr3.3:79627336-79627355 | None:intergenic | 40.0% | |
| ATCTTGTACTGAAGCTTGGA+GGG | + | chr3.3:79627377-79627396 | None:intergenic | 40.0% | |
| CCAAATCTTGTACTGAAGCT+TGG | + | chr3.3:79627381-79627400 | None:intergenic | 40.0% | |
| CCAAGCTTCAGTACAAGATT+TGG | - | chr3.3:79627378-79627397 | MS.gene049298:CDS | 40.0% | |
| GCACACTATGCATTTGTGAA+TGG | - | chr3.3:79627097-79627116 | MS.gene049298:CDS | 40.0% | |
| GCAGATAAGTACAACTATGC+TGG | - | chr3.3:79627346-79627365 | MS.gene049298:CDS | 40.0% | |
| GGAAGAGAAATAGTGAGAAG+TGG | + | chr3.3:79627069-79627088 | None:intergenic | 40.0% | |
| GGTTGGAGTAAGAGATTTAG+AGG | + | chr3.3:79627290-79627309 | None:intergenic | 40.0% | |
| TCACAAATGCATAGTGTGCT+AGG | + | chr3.3:79627096-79627115 | None:intergenic | 40.0% | |
| ! | TTCAAGGTAGTGTTGTTGGT+TGG | + | chr3.3:79627307-79627326 | None:intergenic | 40.0% |
| !! | CTCTTTGAGGACCTTTTTCA+AGG | + | chr3.3:79627323-79627342 | None:intergenic | 40.0% |
| AGTCCAAACGCAGACACAAA+GGG | - | chr3.3:79627202-79627221 | MS.gene049298:CDS | 45.0% | |
| ATGCATAGTGTGCTAGGAAG+AGG | + | chr3.3:79627090-79627109 | None:intergenic | 45.0% | |
| GAGCTTGCCACTAGAGAAAA+TGG | - | chr3.3:79627508-79627527 | MS.gene049298:CDS | 45.0% | |
| TCAGGAGGGTAAACCAAATC+AGG | + | chr3.3:79627490-79627509 | None:intergenic | 45.0% | |
| ! | TTTTCTCTAGTGGCAAGCTC+AGG | + | chr3.3:79627508-79627527 | None:intergenic | 45.0% |
| AAAGGGCCTAGCTGTGATCA+TGG | - | chr3.3:79627219-79627238 | MS.gene049298:CDS | 50.0% | |
| CAGTCCAAACGCAGACACAA+AGG | - | chr3.3:79627201-79627220 | MS.gene049298:CDS | 50.0% | |
| GCCTAGCTGTGATCATGGTT+GGG | - | chr3.3:79627224-79627243 | MS.gene049298:CDS | 50.0% | |
| GCTGTGATCATGGTTGGGAT+TGG | - | chr3.3:79627229-79627248 | MS.gene049298:CDS | 50.0% | |
| TCCCAACCATGATCACAGCT+AGG | + | chr3.3:79627228-79627247 | None:intergenic | 50.0% | |
| TGCGTTTGGACTGCTAGGAT+TGG | + | chr3.3:79627194-79627213 | None:intergenic | 50.0% | |
| AGGCCCTTTGTGTCTGCGTT+TGG | + | chr3.3:79627208-79627227 | None:intergenic | 55.0% | |
| CTCTAGTGGCAAGCTCAGGA+GGG | + | chr3.3:79627504-79627523 | None:intergenic | 55.0% | |
| GGCCTAGCTGTGATCATGGT+TGG | - | chr3.3:79627223-79627242 | MS.gene049298:CDS | 55.0% | |
| GTGTCTGCGTTTGGACTGCT+AGG | + | chr3.3:79627199-79627218 | None:intergenic | 55.0% | |
| TCTCTAGTGGCAAGCTCAGG+AGG | + | chr3.3:79627505-79627524 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 79627052 | 79627588 | 79627052 | ID=MS.gene049298 |
| chr3.3 | mRNA | 79627052 | 79627588 | 79627052 | ID=MS.gene049298.t1;Parent=MS.gene049298 |
| chr3.3 | exon | 79627052 | 79627588 | 79627052 | ID=MS.gene049298.t1.exon1;Parent=MS.gene049298.t1 |
| chr3.3 | CDS | 79627052 | 79627588 | 79627052 | ID=cds.MS.gene049298.t1;Parent=MS.gene049298.t1 |
| Gene Sequence |
| Protein sequence |
