AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27107

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27107.t1 MTR_1g026410 98.895 543 5 1 1 542 1 543 0.0 1093
MS.gene27107.t1 MTR_3g115050 84.461 547 77 2 1 542 1 544 0.0 966
MS.gene27107.t1 MTR_4g075690 76.667 540 119 2 3 542 11 543 0.0 863
MS.gene27107.t1 MTR_4g049390 74.225 516 133 0 23 538 22 537 0.0 814
MS.gene27107.t1 MTR_4g048350 72.674 516 140 1 23 538 22 536 0.0 793
MS.gene27107.t1 MTR_2g088080 71.455 536 153 0 2 537 4 539 0.0 789
MS.gene27107.t1 MTR_4g132320 70.336 536 158 1 7 542 11 545 0.0 763
MS.gene27107.t1 MTR_4g075690 74.775 444 105 2 3 446 11 447 0.0 685
MS.gene27107.t1 MTR_2g088080 71.397 458 131 0 80 537 1 458 0.0 675
MS.gene27107.t1 MTR_8g479390 60.348 517 202 2 23 538 26 540 0.0 650
MS.gene27107.t1 MTR_5g018270 59.266 518 207 2 21 538 24 537 0.0 625
MS.gene27107.t1 MTR_8g479380 56.415 530 222 5 6 534 13 534 0.0 619
MS.gene27107.t1 MTR_4g083190 56.343 536 222 5 5 532 7 538 0.0 597
MS.gene27107.t1 MTR_3g083440 54.444 540 228 5 1 532 7 536 0.0 577
MS.gene27107.t1 MTR_3g083390 54.444 540 228 5 1 532 7 536 0.0 576
MS.gene27107.t1 MTR_3g083430 54.259 540 229 5 1 532 7 536 0.0 574
MS.gene27107.t1 MTR_3g083400 54.259 540 229 5 1 532 7 536 0.0 573
MS.gene27107.t1 MTR_3g083450 55.470 521 223 4 20 532 18 537 0.0 572
MS.gene27107.t1 MTR_4g083210 53.510 527 240 4 10 532 7 532 0.0 570
MS.gene27107.t1 MTR_3g083410 54.878 492 213 4 49 532 10 500 0.0 531
MS.gene27107.t1 MTR_4g101650 51.554 547 244 6 7 533 6 551 0.0 530
MS.gene27107.t1 MTR_3g008580 48.825 553 261 7 1 532 1 552 1.54e-176 511
MS.gene27107.t1 MTR_7g051440 52.174 529 227 10 25 532 26 549 2.82e-175 508
MS.gene27107.t1 MTR_1g090707 45.404 544 278 8 3 533 1 538 1.14e-156 460
MS.gene27107.t1 MTR_4g133150 58.173 208 68 2 132 337 21 211 6.42e-72 229
MS.gene27107.t1 MTR_5g020620 29.806 567 344 18 3 531 11 561 3.41e-59 206
MS.gene27107.t1 MTR_5g020600 28.345 568 352 16 6 531 12 566 2.55e-57 201
MS.gene27107.t1 MTR_3g462760 30.237 506 308 15 28 497 26 522 1.68e-54 193
MS.gene27107.t1 MTR_5g081810 27.561 537 340 12 33 531 4 529 8.03e-54 191
MS.gene27107.t1 MTR_5g069680 27.119 531 343 14 1 497 4 524 2.97e-51 184
MS.gene27107.t1 MTR_4g015120 27.639 521 345 12 22 511 18 537 2.71e-48 176
MS.gene27107.t1 MTR_8g091390 26.910 576 327 18 23 536 29 572 4.31e-47 173
MS.gene27107.t1 MTR_3g078740 25.894 587 335 20 28 537 26 589 7.63e-47 173
MS.gene27107.t1 MTR_7g458880 26.117 582 364 17 1 531 14 580 8.34e-45 167
MS.gene27107.t1 MTR_3g078700 26.678 581 322 23 5 532 7 536 1.20e-44 166
MS.gene27107.t1 MTR_5g073210 26.132 574 358 16 7 531 4 560 5.83e-41 155
MS.gene27107.t1 MTR_7g058690 26.871 521 323 18 28 497 33 546 9.21e-41 155
MS.gene27107.t1 MTR_8g027375 25.414 543 361 14 3 504 5 544 9.40e-41 155
MS.gene27107.t1 MTR_3g101640 27.675 542 327 17 6 497 11 537 2.76e-40 154
MS.gene27107.t1 MTR_3g071890 26.946 501 318 14 49 508 54 547 7.92e-40 152
MS.gene27107.t1 MTR_2g008330 26.376 527 338 14 49 531 59 579 1.81e-39 152
MS.gene27107.t1 MTR_7g065980 29.225 503 303 20 42 496 39 536 1.84e-39 152
MS.gene27107.t1 MTR_7g062310 25.720 521 328 15 28 497 47 559 7.07e-39 150
MS.gene27107.t1 MTR_7g060460 26.095 548 338 18 8 497 9 547 1.67e-38 149
MS.gene27107.t1 MTR_7g062250 25.439 570 372 17 11 531 13 578 1.75e-38 149
MS.gene27107.t1 MTR_5g083360 25.852 499 316 14 46 497 42 533 1.93e-38 149
MS.gene27107.t1 MTR_3g101635 28.189 486 311 14 49 497 48 532 3.00e-37 145
MS.gene27107.t1 MTR_4g064530 25.000 540 359 17 2 497 14 551 2.39e-36 143
MS.gene27107.t1 MTR_7g065970 26.508 547 329 18 1 491 1 530 3.37e-36 142
MS.gene27107.t1 MTR_3g078730 25.342 584 334 21 5 537 7 539 4.52e-36 142
MS.gene27107.t1 MTR_4g064530 25.000 536 354 18 2 491 14 547 3.84e-34 136
MS.gene27107.t1 MTR_4g019225 24.954 549 357 15 32 531 30 572 3.68e-29 122
MS.gene27107.t1 MTR_4g133160 52.294 109 38 2 413 521 1 95 5.99e-27 104
MS.gene27107.t1 MTR_5g018290 63.636 66 24 0 473 538 5 70 2.53e-24 96.7
MS.gene27107.t1 MTR_5g081790 26.538 260 158 7 256 489 168 420 5.52e-14 74.7
MS.gene27107.t1 MTR_5g081790 24.242 165 116 3 10 166 6 169 3.54e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27107.t1 AT1G76160 84.310 529 78 2 14 542 18 541 0.0 928
MS.gene27107.t1 AT1G41830 81.041 538 96 2 5 542 11 542 0.0 911
MS.gene27107.t1 AT1G21850 78.137 526 113 2 14 539 15 538 0.0 861
MS.gene27107.t1 AT1G21860 73.913 529 135 3 10 538 12 537 0.0 828
MS.gene27107.t1 AT4G22010 71.241 532 152 1 12 542 10 541 0.0 795
MS.gene27107.t1 AT4G38420 67.890 545 169 3 3 541 5 549 0.0 760
MS.gene27107.t1 AT4G28090 62.454 538 186 7 12 538 9 541 0.0 689
MS.gene27107.t1 AT5G66920 59.266 518 209 1 21 538 30 545 0.0 651
MS.gene27107.t1 AT4G38420 66.247 477 155 3 3 473 5 481 0.0 639
MS.gene27107.t1 AT1G55570 56.410 546 225 5 1 537 5 546 0.0 607
MS.gene27107.t1 AT4G38420 66.444 450 145 3 3 446 5 454 0.0 601
MS.gene27107.t1 AT3G13390 58.046 522 209 4 24 536 24 544 0.0 591
MS.gene27107.t1 AT3G13400 54.833 538 234 4 7 536 6 542 0.0 585
MS.gene27107.t1 AT3G13400 54.833 538 234 4 7 536 6 542 0.0 585
MS.gene27107.t1 AT1G55560 54.089 538 238 4 7 536 5 541 0.0 582
MS.gene27107.t1 AT4G37160 54.802 531 233 4 14 542 16 541 0.0 581
MS.gene27107.t1 AT2G23630 55.682 528 231 2 13 540 15 539 0.0 579
MS.gene27107.t1 AT2G23630 55.682 528 231 2 13 540 20 544 0.0 579
MS.gene27107.t1 AT5G48450 51.763 539 246 9 7 533 8 544 0.0 548
MS.gene27107.t1 AT5G48450 51.571 541 248 9 7 535 8 546 0.0 548
MS.gene27107.t1 AT4G12420 50.906 552 247 8 1 532 1 548 0.0 535
MS.gene27107.t1 AT4G12420 50.906 552 247 8 1 532 1 548 0.0 535
MS.gene27107.t1 AT5G51480 50.000 530 244 8 23 532 23 551 0.0 526
MS.gene27107.t1 AT1G75790 50.462 541 252 5 10 534 4 544 0.0 521
MS.gene27107.t1 AT4G25240 50.000 552 255 8 1 532 1 551 1.68e-178 516
MS.gene27107.t1 AT4G25240 50.000 552 255 8 1 532 1 551 1.68e-178 516
MS.gene27107.t1 AT4G37160 55.172 435 192 3 14 446 16 449 2.22e-167 482
MS.gene27107.t1 AT4G39830 27.698 556 336 14 2 504 13 555 5.76e-56 197
MS.gene27107.t1 AT5G21105 27.148 582 336 19 12 532 22 576 1.05e-53 191
MS.gene27107.t1 AT5G58910 28.785 535 328 16 7 497 8 533 3.45e-53 190
MS.gene27107.t1 AT2G38080 27.357 541 357 13 23 531 22 558 1.50e-52 188
MS.gene27107.t1 AT5G58910 28.785 535 327 17 7 497 8 532 1.55e-51 185
MS.gene27107.t1 AT5G21105 27.829 539 306 18 12 489 22 538 1.85e-51 184
MS.gene27107.t1 AT5G21100 27.143 560 323 19 32 532 28 561 3.83e-50 181
MS.gene27107.t1 AT5G03260 27.641 568 354 17 5 531 6 557 4.74e-50 181
MS.gene27107.t1 AT5G01190 27.087 539 353 12 28 531 25 558 1.37e-48 177
MS.gene27107.t1 AT5G01190 27.237 514 342 11 49 531 32 544 4.78e-47 172
MS.gene27107.t1 AT5G58910 29.472 492 288 16 49 497 14 489 6.01e-46 169
MS.gene27107.t1 AT4G39830 27.549 461 272 12 96 504 8 458 4.51e-40 152
MS.gene27107.t1 AT4G39830 27.549 461 272 12 96 504 10 460 6.20e-40 152
MS.gene27107.t1 AT2G29130 26.335 543 343 17 2 496 5 538 8.90e-40 152
MS.gene27107.t1 AT2G30210 27.108 498 308 16 46 496 46 535 1.03e-39 152
MS.gene27107.t1 AT2G46570 23.757 543 357 14 28 525 32 562 4.53e-39 150
MS.gene27107.t1 AT5G05390 27.054 499 313 14 42 497 41 531 6.78e-39 150
MS.gene27107.t1 AT3G09220 26.365 531 340 14 1 491 8 527 3.47e-38 148
MS.gene27107.t1 AT5G48100 26.800 500 318 16 49 505 43 537 1.15e-37 146
MS.gene27107.t1 AT1G18140 24.952 525 333 17 28 497 29 547 2.41e-36 143
MS.gene27107.t1 AT5G09360 26.136 528 344 16 7 496 15 534 2.55e-36 142
MS.gene27107.t1 AT5G01040 27.435 503 300 17 39 491 39 526 3.37e-36 142
MS.gene27107.t1 AT5G01050 26.335 543 341 17 39 533 39 570 3.92e-36 142
MS.gene27107.t1 AT4G39830 26.681 461 263 12 96 504 10 447 2.23e-35 139
MS.gene27107.t1 AT2G40370 23.850 587 384 15 1 531 1 580 6.21e-35 139
MS.gene27107.t1 AT5G21105 27.720 386 231 12 173 532 22 385 2.48e-34 134
MS.gene27107.t1 AT5G07130 24.955 553 333 18 14 504 10 542 5.59e-34 136
MS.gene27107.t1 AT2G46570 24.017 483 316 15 85 525 4 477 5.72e-30 123
MS.gene27107.t1 AT3G09220 25.814 430 278 11 101 491 3 430 1.94e-26 112
MS.gene27107.t1 AT5G60020 25.846 325 206 7 1 306 1 309 1.80e-25 110
MS.gene27107.t1 AT5G60020 25.846 325 206 7 1 306 1 309 1.87e-25 110
MS.gene27107.t1 AT5G60020 26.568 271 179 5 49 306 14 277 2.85e-24 107
MS.gene27107.t1 AT5G07130 24.790 476 276 18 91 504 2 457 3.52e-23 103
MS.gene27107.t1 AT5G07130 24.790 476 276 18 91 504 2 457 3.52e-23 103

Find 166 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGGATGTCTTCATCATTT+TGG 0.141546 1.1:-189954 None:intergenic
GGAACTTGAGGTCAGAATTT+TGG 0.153063 1.1:+190230 MS.gene27107:CDS
CACAAAGCTGCTGGTGGTTT+TGG 0.164848 1.1:+188888 MS.gene27107:CDS
TGCTGTCAGGTTTGTCCTAA+TGG 0.243582 1.1:-189644 None:intergenic
AACTCTGCAGGTCCAGTTTC+AGG 0.260810 1.1:+189565 MS.gene27107:CDS
ATGTTACAGGAAAGACTTAC+AGG 0.284854 1.1:+189296 MS.gene27107:intron
GGCTACTCTTTCTTTGTTGT+TGG 0.288891 1.1:+189994 MS.gene27107:CDS
TTCTATTATTTCCCATCTCT+TGG 0.289041 1.1:+188861 MS.gene27107:CDS
AGTTCAGCTGGACAAGTTAA+TGG 0.296097 1.1:+189742 MS.gene27107:CDS
CCACAAAGTAATGCATTCTT+AGG 0.312465 1.1:-190331 None:intergenic
GACAATGGACAAGTGATAAA+AGG 0.313235 1.1:+190107 MS.gene27107:CDS
CGTATGAGTTCCTTCTTTGC+TGG 0.315087 1.1:-188752 None:intergenic
GGTGATTACACTGTCCTTAT+TGG 0.319377 1.1:+188966 MS.gene27107:CDS
GGTTCAAGGACCAATCAATT+TGG 0.323811 1.1:-189612 None:intergenic
AGTAATGCATTCTTAGGAAT+AGG 0.325123 1.1:-190325 None:intergenic
GGTCTATCAGAGATGCTTCC+AGG 0.328166 1.1:-189859 None:intergenic
TTACTTTGTGGTAGGGCAAC+TGG 0.340111 1.1:+190343 MS.gene27107:CDS
TCGAAGGGAACTGGAATTCT+TGG 0.348615 1.1:-188933 None:intergenic
CCGCAAGGATCATACCATTA+TGG 0.358471 1.1:+189685 MS.gene27107:CDS
GATCCTTGCGGGTTTGGTCT+TGG 0.363379 1.1:-189673 None:intergenic
GCTACTCTTTCTTTGTTGTT+GGG 0.364251 1.1:+189995 MS.gene27107:CDS
CCTAAGAATGCATTACTTTG+TGG 0.376644 1.1:+190331 MS.gene27107:CDS
CTTATCAATGGTCGTGGGTC+TGG 0.377274 1.1:+189141 MS.gene27107:CDS
AAGAAGTCAGCCAGCTTAAG+AGG 0.378912 1.1:-189814 None:intergenic
TCCACTTGGTGTTCGCCAAC+AGG 0.379493 1.1:+187748 MS.gene27107:CDS
GTCTTGGTCCACTTGCTGTC+AGG 0.380810 1.1:-189657 None:intergenic
CATAATGGTATGATCCTTGC+GGG 0.382551 1.1:-189684 None:intergenic
CTTCAGAGCTATCATCTTGA+TGG 0.384388 1.1:+189973 MS.gene27107:CDS
TGAACCAAGCCCGCTCCATT+AGG 0.386633 1.1:+189629 MS.gene27107:CDS
CTTCAAGTGAAAGATCAAAT+TGG 0.388160 1.1:+188834 MS.gene27107:CDS
TGGTATGATCCTTGCGGGTT+TGG 0.392790 1.1:-189679 None:intergenic
CAAAACCACCAGCAGCTTTG+TGG 0.393298 1.1:-188887 None:intergenic
ACACTGTCCTTATTGGTGAT+TGG 0.395975 1.1:+188973 MS.gene27107:CDS
TCATACGAAGATGGAGTGTT+TGG 0.398452 1.1:+188768 MS.gene27107:CDS
GTTGCTGTAGCGAAGAATTC+CGG 0.398646 1.1:-189545 None:intergenic
CTTGTTTCCGTTATCAAGTT+GGG 0.404501 1.1:-189097 None:intergenic
ACTTGTACCAATCACCAATA+AGG 0.411862 1.1:-188980 None:intergenic
ATGTATGTAAAGTTCTTTCC+AGG 0.413929 1.1:-188810 None:intergenic
ATCCATACAGGTTCTTCAAC+TGG 0.414214 1.1:+187702 MS.gene27107:CDS
GTAATGCATTCTTAGGAATA+GGG 0.417767 1.1:-190324 None:intergenic
ATTTGGATGGTGGGTCCACC+AGG 0.418556 1.1:-189595 None:intergenic
TTGTCCTAATGGAGCGGGCT+TGG 0.419822 1.1:-189633 None:intergenic
TTAAGTTTATCATGCCATAA+TGG 0.419861 1.1:-189699 None:intergenic
GAATGTCTGGTCCTGGGAAT+TGG 0.420914 1.1:-187911 None:intergenic
AATGGAGCGGGCTTGGTTCA+AGG 0.421159 1.1:-189626 None:intergenic
CCTGATGGAATTCTTATCAA+TGG 0.421680 1.1:+189129 MS.gene27107:CDS
GCGGGATCATCGAAGGGAAC+TGG 0.422110 1.1:-188942 None:intergenic
CTGGAATTCTTGGCCTACTT+AGG 0.428217 1.1:-188923 None:intergenic
TAGAATATCAAATGTGGGAT+TGG 0.430691 1.1:+189321 MS.gene27107:CDS
GCCAGCTTAAGAGGAGTATC+GGG 0.437831 1.1:-189805 None:intergenic
AAGTTCTTTCCAGGTGGAAT+GGG 0.445944 1.1:-188801 None:intergenic
GTTCAGCTGGACAAGTTAAT+GGG 0.447873 1.1:+189743 MS.gene27107:CDS
CAAGGGAGGAGTAGGTGGTT+TGG 0.449186 1.1:-189414 None:intergenic
AGCCAGCTTAAGAGGAGTAT+CGG 0.452171 1.1:-189806 None:intergenic
TAGATAGCAGTCCATGACTT+TGG 0.469788 1.1:-190181 None:intergenic
TATTATGTGACAGGATTTGA+AGG 0.472072 1.1:+189074 MS.gene27107:intron
TGGATGGTGGGTCCACCAGG+GGG 0.473394 1.1:-189592 None:intergenic
GACCCACCATCCAAATTGAT+TGG 0.477439 1.1:+189602 MS.gene27107:CDS
CAGTGAAATGGCACTGAACT+TGG 0.480159 1.1:+187622 None:intergenic
CTTGGATTCCACAAAGCTGC+TGG 0.481877 1.1:+188879 MS.gene27107:CDS
TTTGTCACAGAATGAATGTC+TGG 0.484656 1.1:-187924 None:intergenic
CAGGTTTGTCCTAATGGAGC+GGG 0.488484 1.1:-189638 None:intergenic
AAAGTTCTTTCCAGGTGGAA+TGG 0.489538 1.1:-188802 None:intergenic
GTGGTAGCTAAGTCATCAAA+GGG 0.489623 1.1:-190380 None:intergenic
TTGAAGGCCCAACTTGATAA+CGG 0.491854 1.1:+189090 MS.gene27107:CDS
CCCCGATACTCCTCTTAAGC+TGG 0.492307 1.1:+189804 MS.gene27107:CDS
CCATTGATAAGAATTCCATC+AGG 0.493362 1.1:-189129 None:intergenic
ACTTGTTTCCGTTATCAAGT+TGG 0.493576 1.1:-189098 None:intergenic
ATTCCGGTGGAGGTGAGTAC+AGG 0.496771 1.1:-189529 None:intergenic
CTCCTCCCTTGACGTTCACG+TGG 0.496861 1.1:+189426 MS.gene27107:CDS
ACAGAATGAATGTCTGGTCC+TGG 0.504301 1.1:-187918 None:intergenic
ATAGCAGGTGTTTACCGTCC+TGG 0.506103 1.1:+189841 MS.gene27107:CDS
ACTAACCATGAAATGAGAAA+AGG 0.507335 1.1:-187987 None:intergenic
GGAATGGAGTCCAGCAAAGA+AGG 0.507749 1.1:+188742 MS.gene27107:intron
TTCCAGTTGAAGAACCTGTA+TGG 0.507790 1.1:-187704 None:intergenic
GGGAGGAGTAGGTGGTTTGG+AGG 0.508210 1.1:-189411 None:intergenic
TATCAATGTCTTCAACAGCT+TGG 0.512023 1.1:+187959 MS.gene27107:CDS
GGAATTCTTATCAATGGTCG+TGG 0.513900 1.1:+189135 MS.gene27107:CDS
AGAATGCATTACTTTGTGGT+AGG 0.515449 1.1:+190335 MS.gene27107:CDS
CTGCGTCTCTAAGATTGTAC+TGG 0.516471 1.1:-190132 None:intergenic
TTGGATGGTGGGTCCACCAG+GGG 0.516542 1.1:-189593 None:intergenic
CAAGATGAAGCTGGTAGAAG+TGG 0.521035 1.1:+189375 MS.gene27107:CDS
AACAAGTTACCTTTCCCTGA+TGG 0.521178 1.1:+189114 MS.gene27107:CDS
GAGGAATTAGAATCCTAAGT+AGG 0.523452 1.1:+188910 MS.gene27107:CDS
GCTGACTTCTTCAAGATAGC+AGG 0.524240 1.1:+189826 MS.gene27107:CDS
TTCAACTGGAACGTTACCTA+TGG 0.528833 1.1:+187716 MS.gene27107:CDS
TTTGGATGGTGGGTCCACCA+GGG 0.529837 1.1:-189594 None:intergenic
TTGTTGCTGAAGATCCATAC+AGG 0.530698 1.1:+187690 MS.gene27107:CDS
GAATGCATTACTTTGTGGTA+GGG 0.534147 1.1:+190336 MS.gene27107:CDS
GGATTCCACAAAGCTGCTGG+TGG 0.534304 1.1:+188882 MS.gene27107:CDS
GGAGGAGTAGGTGGTTTGGA+GGG 0.534506 1.1:-189410 None:intergenic
ATCAATGGTCGTGGGTCTGG+AGG 0.535103 1.1:+189144 MS.gene27107:CDS
CTTATCAATGGCCAATTCCC+AGG 0.536067 1.1:+187900 MS.gene27107:CDS
CTTCGCTACAGCAACTCTGC+AGG 0.536476 1.1:+189553 MS.gene27107:CDS
ATGATTAGTTACCGTGTGGT+TGG 0.536620 1.1:-189003 None:intergenic
TTTGGGCACGACAATATCTC+GGG 0.539491 1.1:+190248 MS.gene27107:CDS
TATGGTGACATTTATCCACT+TGG 0.540771 1.1:+187734 MS.gene27107:CDS
CATCAAGATGATAGCTCTGA+AGG 0.545903 1.1:-189972 None:intergenic
TATGTTGCACTTGACAATGT+AGG 0.546497 1.1:+190202 MS.gene27107:CDS
TCTGATAGACCTAGTGGTGG+TGG 0.549192 1.1:+189871 MS.gene27107:CDS
AATCCTGTACTCACCTCCAC+CGG 0.553249 1.1:+189526 MS.gene27107:CDS
ATCACCAGCGGGATCATCGA+AGG 0.554567 1.1:-188949 None:intergenic
CAGGCTTAGAATATCAAATG+TGG 0.556229 1.1:+189315 MS.gene27107:CDS
ATTGTGCTATCAAGTTCAGC+TGG 0.557942 1.1:+189730 MS.gene27107:CDS
AGAAGGAACTCATACGAAGA+TGG 0.560891 1.1:+188759 MS.gene27107:CDS
GACCAATCAATTTGGATGGT+GGG 0.562075 1.1:-189604 None:intergenic
AGCAACAATAGTAGTAGTGA+TGG 0.562486 1.1:-187675 None:intergenic
ATGCAGGGAATACTTATCAA+TGG 0.562736 1.1:+187888 MS.gene27107:intron
CAGAATGAATGTCTGGTCCT+GGG 0.565547 1.1:-187917 None:intergenic
GCTTTGTGGAATCCAAGAGA+TGG 0.565601 1.1:-188873 None:intergenic
CAAGGACCAATCAATTTGGA+TGG 0.569010 1.1:-189608 None:intergenic
AGGACAAACCTGACAGCAAG+TGG 0.574032 1.1:+189649 MS.gene27107:CDS
GTGAACGTCAAGGGAGGAGT+AGG 0.576942 1.1:-189422 None:intergenic
CTAGTAACCGCGGATCAACC+TGG 0.581592 1.1:+189466 MS.gene27107:CDS
GGACCAATCAATTTGGATGG+TGG 0.582450 1.1:-189605 None:intergenic
CTCAGGTATATCCAAAGTCA+TGG 0.583355 1.1:+190170 MS.gene27107:CDS
GGTGGAGGTGAGTACAGGAT+TGG 0.585429 1.1:-189524 None:intergenic
GTAGCGAAGAATTCCGGTGG+AGG 0.585607 1.1:-189539 None:intergenic
ACAACATGTCCCATTCCACC+TGG 0.586121 1.1:+188792 MS.gene27107:CDS
CTTTGTGGAATCCAAGAGAT+GGG 0.586569 1.1:-188872 None:intergenic
ACCTGTTGGCGAACACCAAG+TGG 0.587332 1.1:-187749 None:intergenic
GTTCCCTTCGATGATCCCGC+TGG 0.588900 1.1:+188945 MS.gene27107:CDS
ATGTAGGAATGTGGAACTTG+AGG 0.589636 1.1:+190218 MS.gene27107:CDS
ACAGAATCACAAGATGAAGC+TGG 0.591529 1.1:+189366 MS.gene27107:CDS
AGGCTTAGAATATCAAATGT+GGG 0.595758 1.1:+189316 MS.gene27107:CDS
TTGACCCACGTGAACGTCAA+GGG 0.599164 1.1:-189431 None:intergenic
AATGTTGCATGCAGTATGGA+TGG 0.602475 1.1:+190082 MS.gene27107:intron
ATAAGAATTCCATCAGGGAA+AGG 0.603702 1.1:-189123 None:intergenic
TCAGGTTTGTCCTAATGGAG+CGG 0.604135 1.1:-189639 None:intergenic
AAGTGGATAAATGTCACCAT+AGG 0.607359 1.1:-187732 None:intergenic
AGGACAGTGTAATCACCAGC+GGG 0.611484 1.1:-188960 None:intergenic
CCATAATGGTATGATCCTTG+CGG 0.613798 1.1:-189685 None:intergenic
TACAGGATTGGTAAATCGAG+AGG 0.614756 1.1:-189512 None:intergenic
AACGTCAAGGGAGGAGTAGG+TGG 0.614850 1.1:-189419 None:intergenic
TCATCTCTCAATGTGGAGCA+AGG 0.615730 1.1:+189168 MS.gene27107:CDS
AGATAAATACCACCACCACT+AGG 0.622790 1.1:-189880 None:intergenic
ATTGACCCACGTGAACGTCA+AGG 0.633112 1.1:-189432 None:intergenic
TAGTAGTAGTGATGGCAAAG+AGG 0.634339 1.1:-187667 None:intergenic
GCAGTATGGATGGAGGACAA+TGG 0.636774 1.1:+190092 MS.gene27107:intron
ATGAAGCTGGTAGAAGTGGA+AGG 0.637021 1.1:+189379 MS.gene27107:CDS
TCCTCCCTTGACGTTCACGT+GGG 0.641683 1.1:+189427 MS.gene27107:CDS
ATCTCTGATAGACCTAGTGG+TGG 0.645124 1.1:+189868 MS.gene27107:CDS
TATGTAAAGTTCTTTCCAGG+TGG 0.645224 1.1:-188807 None:intergenic
GAATTCTTATCAATGGTCGT+GGG 0.645558 1.1:+189136 MS.gene27107:CDS
AGTTTCGCGTTCCACCACTC+AGG 0.647895 1.1:+190153 MS.gene27107:CDS
GGACCAAGACCAAACCCGCA+AGG 0.650703 1.1:+189670 MS.gene27107:CDS
TCACCAGCGGGATCATCGAA+GGG 0.652297 1.1:-188948 None:intergenic
CACTTGACAATGTAGGAATG+TGG 0.655047 1.1:+190209 MS.gene27107:CDS
CATTGATAAGAATTCCATCA+GGG 0.656365 1.1:-189128 None:intergenic
TATCAGAGATGCTTCCAGGA+CGG 0.660561 1.1:-189855 None:intergenic
GTCTTGACCAGGTTGATCCG+CGG 0.661746 1.1:-189473 None:intergenic
ACAATATAATAGTCTTGACC+AGG 0.663963 1.1:-189484 None:intergenic
TGGAGGATCATCTCTCAATG+TGG 0.668048 1.1:+189161 MS.gene27107:CDS
AGCATCTCTGATAGACCTAG+TGG 0.673066 1.1:+189865 MS.gene27107:CDS
TTGGTACAAGTCCAACCACA+CGG 0.685221 1.1:+188992 MS.gene27107:CDS
CCAGCTTAAGAGGAGTATCG+GGG 0.687155 1.1:-189804 None:intergenic
ACCCACGTGAACGTCAAGGG+AGG 0.690184 1.1:-189428 None:intergenic
AAGGACAGTGTAATCACCAG+CGG 0.694966 1.1:-188961 None:intergenic
GCTGTAGCGAAGAATTCCGG+TGG 0.700350 1.1:-189542 None:intergenic
CTTTGGATATACCTGAGTGG+TGG 0.703031 1.1:-190164 None:intergenic
ATACTCAGTTCTAGTAACCG+CGG 0.703123 1.1:+189456 MS.gene27107:CDS
CAGCTTAAGAGGAGTATCGG+GGG 0.703277 1.1:-189803 None:intergenic
GTTGCATGCAGTATGGATGG+AGG 0.723579 1.1:+190085 MS.gene27107:intron
ATGGCATGATAAACTTAACG+AGG 0.737321 1.1:+189704 MS.gene27107:CDS
TGGTAGCTAAGTCATCAAAG+GGG 0.773118 1.1:-190379 None:intergenic
TGACTTTGGATATACCTGAG+TGG 0.778186 1.1:-190167 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATATAACAAATTAAGTT+TGG - chr1.1:187857-187876 None:intergenic 15.0%
!!! CACTTTTATATTCATTTATA+TGG + chr1.1:188370-188389 MS.gene27107:intron 15.0%
!! AAAGCATGAATAAAGTAAAT+GGG - chr1.1:188539-188558 None:intergenic 20.0%
!! AATGAAAATGATCTATATGA+TGG - chr1.1:190034-190053 None:intergenic 20.0%
!! CACAATAATTTAAAAAGTAG+TGG - chr1.1:188637-188656 None:intergenic 20.0%
!! TATATTCATTTATATGGAGA+TGG + chr1.1:188376-188395 MS.gene27107:intron 20.0%
!! TCAATTCCAAATTAAGTAAT+TGG + chr1.1:188427-188446 MS.gene27107:intron 20.0%
!! TCATTTATTAATCAATTGCA+AGG - chr1.1:188694-188713 None:intergenic 20.0%
!! TGAAATATAAATATACCTGT+TGG - chr1.1:187766-187785 None:intergenic 20.0%
!!! TTGACACAACTAATATTTTT+TGG + chr1.1:189261-189280 MS.gene27107:intron 20.0%
! AAAATGATTCAGATCCAATA+AGG - chr1.1:187803-187822 None:intergenic 25.0%
! AAGCATGAATAAAGTAAATG+GGG - chr1.1:188538-188557 None:intergenic 25.0%
! ACGTGAAATAGTAGTATATT+AGG + chr1.1:188609-188628 MS.gene27107:intron 25.0%
! AGATAATTAGCTCAATTTGA+TGG - chr1.1:188581-188600 None:intergenic 25.0%
! ATTGTATTGTATTATGTGAC+AGG + chr1.1:189065-189084 MS.gene27107:intron 25.0%
! CAAAGCATGAATAAAGTAAA+TGG - chr1.1:188540-188559 None:intergenic 25.0%
! CAAATTTAGTACTAATGTTG+AGG - chr1.1:189244-189263 None:intergenic 25.0%
! CGTGAAATAGTAGTATATTA+GGG + chr1.1:188610-188629 MS.gene27107:intron 25.0%
! GACTCATATAATGTTTCATT+TGG + chr1.1:189214-189233 MS.gene27107:intron 25.0%
! GATGAAAAAAGAAATGTGAA+AGG - chr1.1:188018-188037 None:intergenic 25.0%
! TTAAGTTTATCATGCCATAA+TGG - chr1.1:189702-189721 None:intergenic 25.0%
!! TATAGTATGTGTGTTTTGTA+AGG - chr1.1:188281-188300 None:intergenic 25.0%
!! TGTTATTTACACATAGATAG+AGG - chr1.1:188049-188068 None:intergenic 25.0%
!!! CCTCAAAAGTCTTTTTAATT+GGG + chr1.1:188508-188527 MS.gene27107:intron 25.0%
!!! TATATGAGTCTTATTCATGT+AGG - chr1.1:189204-189223 None:intergenic 25.0%
ACATGACATAAAAACAGGAA+TGG + chr1.1:188726-188745 MS.gene27107:intron 30.0%
ACATGACATGACATAAAAAC+AGG + chr1.1:188721-188740 MS.gene27107:intron 30.0%
ACTAACCATGAAATGAGAAA+AGG - chr1.1:187990-188009 None:intergenic 30.0%
ACTACTATTTCACGTAATCA+AGG - chr1.1:188604-188623 None:intergenic 30.0%
AGGCTTAGAATATCAAATGT+GGG + chr1.1:189316-189335 MS.gene27107:CDS 30.0%
AGTAATGCATTCTTAGGAAT+AGG - chr1.1:190328-190347 None:intergenic 30.0%
ATATGAAGATATCAAACGTG+TGG - chr1.1:188151-188170 None:intergenic 30.0%
ATGTATGTAAAGTTCTTTCC+AGG - chr1.1:188813-188832 None:intergenic 30.0%
CATTGATAAGAATTCCATCA+GGG - chr1.1:189131-189150 None:intergenic 30.0%
CTTCAAGTGAAAGATCAAAT+TGG + chr1.1:188834-188853 MS.gene27107:CDS 30.0%
CTTTCATCCTTATACCTTAT+TGG + chr1.1:187786-187805 MS.gene27107:intron 30.0%
GTAATGCATTCTTAGGAATA+GGG - chr1.1:190327-190346 None:intergenic 30.0%
TAGAATATCAAATGTGGGAT+TGG + chr1.1:189321-189340 MS.gene27107:CDS 30.0%
TATTATGTGACAGGATTTGA+AGG + chr1.1:189074-189093 MS.gene27107:intron 30.0%
TTCAGATCCAATAAGGTATA+AGG - chr1.1:187796-187815 None:intergenic 30.0%
TTCTATTATTTCCCATCTCT+TGG + chr1.1:188861-188880 MS.gene27107:CDS 30.0%
! ACAATATAATAGTCTTGACC+AGG - chr1.1:189487-189506 None:intergenic 30.0%
! ACCTAGTACTACTACATTTT+GGG + chr1.1:188186-188205 MS.gene27107:intron 30.0%
! CCCAATTAAAAAGACTTTTG+AGG - chr1.1:188511-188530 None:intergenic 30.0%
! GCCTCAAAAGTCTTTTTAAT+TGG + chr1.1:188507-188526 MS.gene27107:intron 30.0%
! TACCTAGTACTACTACATTT+TGG + chr1.1:188185-188204 MS.gene27107:intron 30.0%
!! CAAAAGTCTTTTTAATTGGG+TGG + chr1.1:188511-188530 MS.gene27107:intron 30.0%
!!! ATTAAAAAGACTTTTGAGGC+AGG - chr1.1:188507-188526 None:intergenic 30.0%
!!! ATTTTGGGATGTTTGTTTGT+TGG + chr1.1:188201-188220 MS.gene27107:intron 30.0%
!!! TTTTGGGATGTTTGTTTGTT+GGG + chr1.1:188202-188221 MS.gene27107:intron 30.0%
AAGTGGATAAATGTCACCAT+AGG - chr1.1:187735-187754 None:intergenic 35.0%
ACTTGTACCAATCACCAATA+AGG - chr1.1:188983-189002 None:intergenic 35.0%
ACTTGTTTCCGTTATCAAGT+TGG - chr1.1:189101-189120 None:intergenic 35.0%
AGAATGCATTACTTTGTGGT+AGG + chr1.1:190335-190354 MS.gene27107:CDS 35.0%
AGCAACAATAGTAGTAGTGA+TGG - chr1.1:187678-187697 None:intergenic 35.0%
ATAAGAATTCCATCAGGGAA+AGG - chr1.1:189126-189145 None:intergenic 35.0%
ATGCAGGGAATACTTATCAA+TGG + chr1.1:187888-187907 MS.gene27107:intron 35.0%
ATGGCATGATAAACTTAACG+AGG + chr1.1:189704-189723 MS.gene27107:CDS 35.0%
ATGTTACAGGAAAGACTTAC+AGG + chr1.1:189296-189315 MS.gene27107:intron 35.0%
CAGGCTTAGAATATCAAATG+TGG + chr1.1:189315-189334 MS.gene27107:CDS 35.0%
CCACAAAGTAATGCATTCTT+AGG - chr1.1:190334-190353 None:intergenic 35.0%
CCATTGATAAGAATTCCATC+AGG - chr1.1:189132-189151 None:intergenic 35.0%
CCTAAGAATGCATTACTTTG+TGG + chr1.1:190331-190350 MS.gene27107:CDS 35.0%
CCTGATGGAATTCTTATCAA+TGG + chr1.1:189129-189148 MS.gene27107:CDS 35.0%
CTTGTTTCCGTTATCAAGTT+GGG - chr1.1:189100-189119 None:intergenic 35.0%
GAATGCATTACTTTGTGGTA+GGG + chr1.1:190336-190355 MS.gene27107:CDS 35.0%
GAATTCTTATCAATGGTCGT+GGG + chr1.1:189136-189155 MS.gene27107:CDS 35.0%
GACAATGGACAAGTGATAAA+AGG + chr1.1:190107-190126 MS.gene27107:CDS 35.0%
GAGGAATTAGAATCCTAAGT+AGG + chr1.1:188910-188929 MS.gene27107:CDS 35.0%
GCATGTTATGTTGTTAGAGT+TGG + chr1.1:188088-188107 MS.gene27107:intron 35.0%
TATCAATGTCTTCAACAGCT+TGG + chr1.1:187959-187978 MS.gene27107:CDS 35.0%
TATCTCTTGTAGTACTATGC+TGG + chr1.1:188307-188326 MS.gene27107:intron 35.0%
TATGAATGTTGCATGCAGTA+TGG + chr1.1:190078-190097 MS.gene27107:intron 35.0%
TATGGTGACATTTATCCACT+TGG + chr1.1:187734-187753 MS.gene27107:CDS 35.0%
TATGTAAAGTTCTTTCCAGG+TGG - chr1.1:188810-188829 None:intergenic 35.0%
TATGTTGCACTTGACAATGT+AGG + chr1.1:190202-190221 MS.gene27107:CDS 35.0%
TCCCAAAATGTAGTAGTACT+AGG - chr1.1:188190-188209 None:intergenic 35.0%
TCCTGTAACATCAATGACAA+GGG - chr1.1:189287-189306 None:intergenic 35.0%
TGCTGGCCAATTACTTAATT+TGG - chr1.1:188436-188455 None:intergenic 35.0%
TTCCTGTAACATCAATGACA+AGG - chr1.1:189288-189307 None:intergenic 35.0%
TTTACACATAGATAGAGGCT+AGG - chr1.1:188044-188063 None:intergenic 35.0%
TTTGTCACAGAATGAATGTC+TGG - chr1.1:187927-187946 None:intergenic 35.0%
! ACGAACCTTTTCTCATTTCA+TGG + chr1.1:187982-188001 MS.gene27107:CDS 35.0%
! ATGTTTTGTGACAAACATGC+AGG + chr1.1:187872-187891 MS.gene27107:intron 35.0%
! GAACTTGAGGTCAGAATTTT+GGG + chr1.1:190231-190250 MS.gene27107:CDS 35.0%
! GAAGGATGTCTTCATCATTT+TGG - chr1.1:189957-189976 None:intergenic 35.0%
! GTAAATGATTAGTTACCGTG+TGG - chr1.1:189010-189029 None:intergenic 35.0%
! TCAATTTGATGGTTAGCTAG+AGG - chr1.1:188570-188589 None:intergenic 35.0%
! TGTTTTGTGACAAACATGCA+GGG + chr1.1:187873-187892 MS.gene27107:intron 35.0%
!! GCTACTCTTTCTTTGTTGTT+GGG + chr1.1:189995-190014 MS.gene27107:CDS 35.0%
AAAAGTGAAGCTAGCAGATC+TGG - chr1.1:188357-188376 None:intergenic 40.0%
AAAGTGAAGCTAGCAGATCT+GGG - chr1.1:188356-188375 None:intergenic 40.0%
AAAGTTCTTTCCAGGTGGAA+TGG - chr1.1:188805-188824 None:intergenic 40.0%
AACAAGTTACCTTTCCCTGA+TGG + chr1.1:189114-189133 MS.gene27107:CDS 40.0%
AAGTTCTTTCCAGGTGGAAT+GGG - chr1.1:188804-188823 None:intergenic 40.0%
AATGTTGCATGCAGTATGGA+TGG + chr1.1:190082-190101 MS.gene27107:intron 40.0%
ACAGAATCACAAGATGAAGC+TGG + chr1.1:189366-189385 MS.gene27107:CDS 40.0%
ACCACTTGTATTGTGTTGTG+TGG + chr1.1:188229-188248 MS.gene27107:intron 40.0%
AGAAGGAACTCATACGAAGA+TGG + chr1.1:188759-188778 MS.gene27107:CDS 40.0%
AGATAAATACCACCACCACT+AGG - chr1.1:189883-189902 None:intergenic 40.0%
AGTTCAGCTGGACAAGTTAA+TGG + chr1.1:189742-189761 MS.gene27107:CDS 40.0%
ATCCATACAGGTTCTTCAAC+TGG + chr1.1:187702-187721 MS.gene27107:CDS 40.0%
ATGTAGGAATGTGGAACTTG+AGG + chr1.1:190218-190237 MS.gene27107:CDS 40.0%
ATTGTGCTATCAAGTTCAGC+TGG + chr1.1:189730-189749 MS.gene27107:CDS 40.0%
CAAGGACCAATCAATTTGGA+TGG - chr1.1:189611-189630 None:intergenic 40.0%
CACTTGACAATGTAGGAATG+TGG + chr1.1:190209-190228 MS.gene27107:CDS 40.0%
CATAATGGTATGATCCTTGC+GGG - chr1.1:189687-189706 None:intergenic 40.0%
CCATAATGGTATGATCCTTG+CGG - chr1.1:189688-189707 None:intergenic 40.0%
CTCAGGTATATCCAAAGTCA+TGG + chr1.1:190170-190189 MS.gene27107:CDS 40.0%
CTTCAGAGCTATCATCTTGA+TGG + chr1.1:189973-189992 MS.gene27107:CDS 40.0%
CTTTGTGGAATCCAAGAGAT+GGG - chr1.1:188875-188894 None:intergenic 40.0%
GACCAATCAATTTGGATGGT+GGG - chr1.1:189607-189626 None:intergenic 40.0%
GGAATTCTTATCAATGGTCG+TGG + chr1.1:189135-189154 MS.gene27107:CDS 40.0%
GGTGATTACACTGTCCTTAT+TGG + chr1.1:188966-188985 MS.gene27107:CDS 40.0%
GGTTCAAGGACCAATCAATT+TGG - chr1.1:189615-189634 None:intergenic 40.0%
GTTCAGCTGGACAAGTTAAT+GGG + chr1.1:189743-189762 MS.gene27107:CDS 40.0%
TAGATAGCAGTCCATGACTT+TGG - chr1.1:190184-190203 None:intergenic 40.0%
TAGTAGTAGTGATGGCAAAG+AGG - chr1.1:187670-187689 None:intergenic 40.0%
TGACTTTGGATATACCTGAG+TGG - chr1.1:190170-190189 None:intergenic 40.0%
TTCAACTGGAACGTTACCTA+TGG + chr1.1:187716-187735 MS.gene27107:CDS 40.0%
TTCCAGTTGAAGAACCTGTA+TGG - chr1.1:187707-187726 None:intergenic 40.0%
TTGAAGGCCCAACTTGATAA+CGG + chr1.1:189090-189109 MS.gene27107:CDS 40.0%
TTGTAGTACTATGCTGGTCA+TGG + chr1.1:188313-188332 MS.gene27107:intron 40.0%
TTGTTGCTGAAGATCCATAC+AGG + chr1.1:187690-187709 MS.gene27107:CDS 40.0%
! ACACTGTCCTTATTGGTGAT+TGG + chr1.1:188973-188992 MS.gene27107:CDS 40.0%
! ATACTCAGTTCTAGTAACCG+CGG + chr1.1:189456-189475 MS.gene27107:CDS 40.0%
! ATGATTAGTTACCGTGTGGT+TGG - chr1.1:189006-189025 None:intergenic 40.0%
! GGAACTTGAGGTCAGAATTT+TGG + chr1.1:190230-190249 MS.gene27107:CDS 40.0%
! GGCTACTCTTTCTTTGTTGT+TGG + chr1.1:189994-190013 MS.gene27107:CDS 40.0%
! TACAGGATTGGTAAATCGAG+AGG - chr1.1:189515-189534 None:intergenic 40.0%
! TCATACGAAGATGGAGTGTT+TGG + chr1.1:188768-188787 MS.gene27107:CDS 40.0%
! TGGCTAGCTAACTAACTAAC+AGG - chr1.1:188131-188150 None:intergenic 40.0%
! TTTTGGGCACGACAATATCT+CGG + chr1.1:190247-190266 MS.gene27107:CDS 40.0%
!! CATCAAGATGATAGCTCTGA+AGG - chr1.1:189975-189994 None:intergenic 40.0%
AAGAAGTCAGCCAGCTTAAG+AGG - chr1.1:189817-189836 None:intergenic 45.0%
AAGGACAGTGTAATCACCAG+CGG - chr1.1:188964-188983 None:intergenic 45.0%
AATTGGCCAGCACTGCAATT+TGG + chr1.1:188444-188463 MS.gene27107:intron 45.0%
ACAGAATGAATGTCTGGTCC+TGG - chr1.1:187921-187940 None:intergenic 45.0%
AGAAAGCCAAATTGCAGTGC+TGG - chr1.1:188453-188472 None:intergenic 45.0%
AGCATCTCTGATAGACCTAG+TGG + chr1.1:189865-189884 MS.gene27107:CDS 45.0%
AGTACTATGCTGGTCATGGT+GGG + chr1.1:188317-188336 MS.gene27107:intron 45.0%
ATCTCTGATAGACCTAGTGG+TGG + chr1.1:189868-189887 MS.gene27107:CDS 45.0%
ATGAAGCTGGTAGAAGTGGA+AGG + chr1.1:189379-189398 MS.gene27107:CDS 45.0%
ATTGTGTTGTGTGGCTGCAA+AGG + chr1.1:188238-188257 MS.gene27107:intron 45.0%
CAAGATGAAGCTGGTAGAAG+TGG + chr1.1:189375-189394 MS.gene27107:CDS 45.0%
CACATAGATAGAGGCTAGGA+TGG - chr1.1:188040-188059 None:intergenic 45.0%
CAGAATGAATGTCTGGTCCT+GGG - chr1.1:187920-187939 None:intergenic 45.0%
CCGCAAGGATCATACCATTA+TGG + chr1.1:189685-189704 MS.gene27107:CDS 45.0%
CGTATGAGTTCCTTCTTTGC+TGG - chr1.1:188755-188774 None:intergenic 45.0%
CTGGAATTCTTGGCCTACTT+AGG - chr1.1:188926-188945 None:intergenic 45.0%
CTTATCAATGGCCAATTCCC+AGG + chr1.1:187900-187919 MS.gene27107:CDS 45.0%
CTTTGGATATACCTGAGTGG+TGG - chr1.1:190167-190186 None:intergenic 45.0%
GACCCACCATCCAAATTGAT+TGG + chr1.1:189602-189621 MS.gene27107:CDS 45.0%
GCCACACAACACAATACAAG+TGG - chr1.1:188233-188252 None:intergenic 45.0%
GCCCTTGTCATTGATGTTAC+AGG + chr1.1:189283-189302 MS.gene27107:intron 45.0%
GCTTTGTGGAATCCAAGAGA+TGG - chr1.1:188876-188895 None:intergenic 45.0%
GGACCAATCAATTTGGATGG+TGG - chr1.1:189608-189627 None:intergenic 45.0%
GTTGCTGTAGCGAAGAATTC+CGG - chr1.1:189548-189567 None:intergenic 45.0%
TAGTACTATGCTGGTCATGG+TGG + chr1.1:188316-188335 MS.gene27107:intron 45.0%
TATCAGAGATGCTTCCAGGA+CGG - chr1.1:189858-189877 None:intergenic 45.0%
TCAGGTTTGTCCTAATGGAG+CGG - chr1.1:189642-189661 None:intergenic 45.0%
TCATCTCTCAATGTGGAGCA+AGG + chr1.1:189168-189187 MS.gene27107:CDS 45.0%
TCGAAGGGAACTGGAATTCT+TGG - chr1.1:188936-188955 None:intergenic 45.0%
TGCTGTCAGGTTTGTCCTAA+TGG - chr1.1:189647-189666 None:intergenic 45.0%
TGGAGGATCATCTCTCAATG+TGG + chr1.1:189161-189180 MS.gene27107:CDS 45.0%
TTACTTTGTGGTAGGGCAAC+TGG + chr1.1:190343-190362 MS.gene27107:CDS 45.0%
TTGGTACAAGTCCAACCACA+CGG + chr1.1:188992-189011 MS.gene27107:CDS 45.0%
TTTGGGCACGACAATATCTC+GGG + chr1.1:190248-190267 MS.gene27107:CDS 45.0%
! AGCCAGCTTAAGAGGAGTAT+CGG - chr1.1:189809-189828 None:intergenic 45.0%
! GCTGACTTCTTCAAGATAGC+AGG + chr1.1:189826-189845 MS.gene27107:CDS 45.0%
!! CTGCGTCTCTAAGATTGTAC+TGG - chr1.1:190135-190154 None:intergenic 45.0%
!!! AAAAGACTTTTGAGGCAGGC+AGG - chr1.1:188503-188522 None:intergenic 45.0%
AACTCTGCAGGTCCAGTTTC+AGG + chr1.1:189565-189584 MS.gene27107:CDS 50.0%
AATCCTGTACTCACCTCCAC+CGG + chr1.1:189526-189545 MS.gene27107:CDS 50.0%
ACAACATGTCCCATTCCACC+TGG + chr1.1:188792-188811 MS.gene27107:CDS 50.0%
AGGACAAACCTGACAGCAAG+TGG + chr1.1:189649-189668 MS.gene27107:CDS 50.0%
AGGACAGTGTAATCACCAGC+GGG - chr1.1:188963-188982 None:intergenic 50.0%
ATAGCAGGTGTTTACCGTCC+TGG + chr1.1:189841-189860 MS.gene27107:CDS 50.0%
ATTGACCCACGTGAACGTCA+AGG - chr1.1:189435-189454 None:intergenic 50.0%
CAAAACCACCAGCAGCTTTG+TGG - chr1.1:188890-188909 None:intergenic 50.0%
CAGGTTTGTCCTAATGGAGC+GGG - chr1.1:189641-189660 None:intergenic 50.0%
CTTATCAATGGTCGTGGGTC+TGG + chr1.1:189141-189160 MS.gene27107:CDS 50.0%
CTTGGATTCCACAAAGCTGC+TGG + chr1.1:188879-188898 MS.gene27107:CDS 50.0%
GAATGTCTGGTCCTGGGAAT+TGG - chr1.1:187914-187933 None:intergenic 50.0%
GCAGTATGGATGGAGGACAA+TGG + chr1.1:190092-190111 MS.gene27107:intron 50.0%
GGAATGGAGTCCAGCAAAGA+AGG + chr1.1:188742-188761 MS.gene27107:intron 50.0%
GGTCTATCAGAGATGCTTCC+AGG - chr1.1:189862-189881 None:intergenic 50.0%
GTTGCATGCAGTATGGATGG+AGG + chr1.1:190085-190104 MS.gene27107:intron 50.0%
GTTGTGTGGCTGCAAAGGAT+AGG + chr1.1:188243-188262 MS.gene27107:intron 50.0%
TCTGATAGACCTAGTGGTGG+TGG + chr1.1:189871-189890 MS.gene27107:CDS 50.0%
TGGTATGATCCTTGCGGGTT+TGG - chr1.1:189682-189701 None:intergenic 50.0%
TTGACCCACGTGAACGTCAA+GGG - chr1.1:189434-189453 None:intergenic 50.0%
! CACAAAGCTGCTGGTGGTTT+TGG + chr1.1:188888-188907 MS.gene27107:CDS 50.0%
!! CAGCTTAAGAGGAGTATCGG+GGG - chr1.1:189806-189825 None:intergenic 50.0%
!! CCAGCTTAAGAGGAGTATCG+GGG - chr1.1:189807-189826 None:intergenic 50.0%
!! GCCAGCTTAAGAGGAGTATC+GGG - chr1.1:189808-189827 None:intergenic 50.0%
!!! AAAGCTGCTGGTGGTTTTGG+AGG + chr1.1:188891-188910 MS.gene27107:CDS 50.0%
AACGTCAAGGGAGGAGTAGG+TGG - chr1.1:189422-189441 None:intergenic 55.0%
ACCTGTTGGCGAACACCAAG+TGG - chr1.1:187752-187771 None:intergenic 55.0%
AGTTTCGCGTTCCACCACTC+AGG + chr1.1:190153-190172 MS.gene27107:CDS 55.0%
ATCAATGGTCGTGGGTCTGG+AGG + chr1.1:189144-189163 MS.gene27107:CDS 55.0%
ATCACCAGCGGGATCATCGA+AGG - chr1.1:188952-188971 None:intergenic 55.0%
ATTCCGGTGGAGGTGAGTAC+AGG - chr1.1:189532-189551 None:intergenic 55.0%
CAAGGGAGGAGTAGGTGGTT+TGG - chr1.1:189417-189436 None:intergenic 55.0%
CCCCGATACTCCTCTTAAGC+TGG + chr1.1:189804-189823 MS.gene27107:CDS 55.0%
CTAGTAACCGCGGATCAACC+TGG + chr1.1:189466-189485 MS.gene27107:CDS 55.0%
CTTCGCTACAGCAACTCTGC+AGG + chr1.1:189553-189572 MS.gene27107:CDS 55.0%
GCTAGCAGATCTGGGTGGAA+TGG - chr1.1:188348-188367 None:intergenic 55.0%
GCTGTAGCGAAGAATTCCGG+TGG - chr1.1:189545-189564 None:intergenic 55.0%
GGATTCCACAAAGCTGCTGG+TGG + chr1.1:188882-188901 MS.gene27107:CDS 55.0%
GGTGGAGGTGAGTACAGGAT+TGG - chr1.1:189527-189546 None:intergenic 55.0%
GTAGCGAAGAATTCCGGTGG+AGG - chr1.1:189542-189561 None:intergenic 55.0%
GTGAACGTCAAGGGAGGAGT+AGG - chr1.1:189425-189444 None:intergenic 55.0%
GTGAAGCTAGCAGATCTGGG+TGG - chr1.1:188353-188372 None:intergenic 55.0%
TCACCAGCGGGATCATCGAA+GGG - chr1.1:188951-188970 None:intergenic 55.0%
TCCTCCCTTGACGTTCACGT+GGG + chr1.1:189427-189446 MS.gene27107:CDS 55.0%
TGAACCAAGCCCGCTCCATT+AGG + chr1.1:189629-189648 MS.gene27107:CDS 55.0%
! AATGGAGCGGGCTTGGTTCA+AGG - chr1.1:189629-189648 None:intergenic 55.0%
! GATCCTTGCGGGTTTGGTCT+TGG - chr1.1:189676-189695 None:intergenic 55.0%
! GGAGGAGTAGGTGGTTTGGA+GGG - chr1.1:189413-189432 None:intergenic 55.0%
! TTGTCCTAATGGAGCGGGCT+TGG - chr1.1:189636-189655 None:intergenic 55.0%
!! ATTTGGATGGTGGGTCCACC+AGG - chr1.1:189598-189617 None:intergenic 55.0%
!! GTCTTGACCAGGTTGATCCG+CGG - chr1.1:189476-189495 None:intergenic 55.0%
!! GTCTTGGTCCACTTGCTGTC+AGG - chr1.1:189660-189679 None:intergenic 55.0%
!! TCCACTTGGTGTTCGCCAAC+AGG + chr1.1:187748-187767 MS.gene27107:CDS 55.0%
!! TTTGGATGGTGGGTCCACCA+GGG - chr1.1:189597-189616 None:intergenic 55.0%
ACCCACGTGAACGTCAAGGG+AGG - chr1.1:189431-189450 None:intergenic 60.0%
CTCCTCCCTTGACGTTCACG+TGG + chr1.1:189426-189445 MS.gene27107:CDS 60.0%
GCGGGATCATCGAAGGGAAC+TGG - chr1.1:188945-188964 None:intergenic 60.0%
GGACCAAGACCAAACCCGCA+AGG + chr1.1:189670-189689 MS.gene27107:CDS 60.0%
GTTCCCTTCGATGATCCCGC+TGG + chr1.1:188945-188964 MS.gene27107:CDS 60.0%
! GGGAGGAGTAGGTGGTTTGG+AGG - chr1.1:189414-189433 None:intergenic 60.0%
!! TTGGATGGTGGGTCCACCAG+GGG - chr1.1:189596-189615 None:intergenic 60.0%
!!! GACTTTTGAGGCAGGCAGGC+AGG - chr1.1:188499-188518 None:intergenic 60.0%
CCAGGGGGTGGACCTGAAAC+TGG - chr1.1:189580-189599 None:intergenic 65.0%
! CCAGTTTCAGGTCCACCCCC+TGG + chr1.1:189577-189596 MS.gene27107:CDS 65.0%
! GTTTCAGGTCCACCCCCTGG+TGG + chr1.1:189580-189599 MS.gene27107:CDS 65.0%
! TTTGAGGCAGGCAGGCAGGC+AGG - chr1.1:188495-188514 None:intergenic 65.0%
!! TGGATGGTGGGTCCACCAGG+GGG - chr1.1:189595-189614 None:intergenic 65.0%
!! ATGGTGGGTCCACCAGGGGG+TGG - chr1.1:189592-189611 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 187629 190387 187629 ID=MS.gene27107
chr1.1 mRNA 187629 190387 187629 ID=MS.gene27107.t1;Parent=MS.gene27107
chr1.1 exon 187629 187769 187629 ID=MS.gene27107.t1.exon1;Parent=MS.gene27107.t1
chr1.1 CDS 187629 187769 187629 ID=cds.MS.gene27107.t1;Parent=MS.gene27107.t1
chr1.1 exon 187894 188003 187894 ID=MS.gene27107.t1.exon2;Parent=MS.gene27107.t1
chr1.1 CDS 187894 188003 187894 ID=cds.MS.gene27107.t1;Parent=MS.gene27107.t1
chr1.1 exon 188743 189013 188743 ID=MS.gene27107.t1.exon3;Parent=MS.gene27107.t1
chr1.1 CDS 188743 189013 188743 ID=cds.MS.gene27107.t1;Parent=MS.gene27107.t1
chr1.1 exon 189087 189189 189087 ID=MS.gene27107.t1.exon4;Parent=MS.gene27107.t1
chr1.1 CDS 189087 189189 189087 ID=cds.MS.gene27107.t1;Parent=MS.gene27107.t1
chr1.1 exon 189305 190016 189305 ID=MS.gene27107.t1.exon5;Parent=MS.gene27107.t1
chr1.1 CDS 189305 190016 189305 ID=cds.MS.gene27107.t1;Parent=MS.gene27107.t1
chr1.1 exon 190096 190387 190096 ID=MS.gene27107.t1.exon6;Parent=MS.gene27107.t1
chr1.1 CDS 190096 190387 190096 ID=cds.MS.gene27107.t1;Parent=MS.gene27107.t1
Gene Sequence

>MS.gene27107

ATGGCACTGAACTTGGTGTTATTATGTGTTAGTGTTTACCTCTTTGCCATCACTACTACTATTGTTGCTGAAGATCCATACAGGTTCTTCAACTGGAACGTTACCTATGGTGACATTTATCCACTTGGTGTTCGCCAACAGGTATATTTATATTTCACTTTCATCCTTATACCTTATTGGATCTGAATCATTTTGATTAATTAATTGATTCATCAACTAGATAGACCAAACTTAATTTGTTATATGTTTTGTGACAAACATGCAGGGAATACTTATCAATGGCCAATTCCCAGGACCAGACATTCATTCTGTGACAAATGATAATCTCATTATCAATGTCTTCAACAGCTTGGACGAACCTTTTCTCATTTCATGGTTAGTTAGTACCTTTCACATTTCTTTTTTCATCCATCCTAGCCTCTATCTATGTGTAAATAACAAAAAGAATTAAACACGAAAGCATGTTATGTTGTTAGAGTTGGTTTCTTTTAATGATGTTCCTGTTAGTTAGTTAGCTAGCCACACGTTTGATATCTTCATATTCATCATATCATACTACCTAGTACTACTACATTTTGGGATGTTTGTTTGTTGGGTCACACCACTTGTATTGTGTTGTGTGGCTGCAAAGGATAGGAAAATGATGAAACCTTACAAAACACACATACTATACTATCTTATCTCTTGTAGTACTATGCTGGTCATGGTGGGTCAATCCATTCCACCCAGATCTGCTAGCTTCACTTTTATATTCATTTATATGGAGATGGAAGACATTTTCACAAGTAATATTAATATTCAATTCCAAATTAAGTAATTGGCCAGCACTGCAATTTGGCTTTCTATACTTGCTTGCTTGCTTGCCTGCCTGCCTGCCTGCCTCAAAAGTCTTTTTAATTGGGTGGACCCCATTTACTTTATTCATGCTTTGCTGTTCTCCTCTAGCTAACCATCAAATTGAGCTAATTATCTCCTTGATTACGTGAAATAGTAGTATATTAGGGACCACTACTTTTTAAATTATTGTGAATTTGTGATAATACTAACTGATAAGACATATAACCTTGCAATTGATTAATAAATGATGACATGACATGACATGACATAAAAACAGGAATGGAGTCCAGCAAAGAAGGAACTCATACGAAGATGGAGTGTTTGGAACAACATGTCCCATTCCACCTGGAAAGAACTTTACATACATACTTCAAGTGAAAGATCAAATTGGTAGTTTCTATTATTTCCCATCTCTTGGATTCCACAAAGCTGCTGGTGGTTTTGGAGGAATTAGAATCCTAAGTAGGCCAAGAATTCCAGTTCCCTTCGATGATCCCGCTGGTGATTACACTGTCCTTATTGGTGATTGGTACAAGTCCAACCACACGGTAACTAATCATTTACTATCTTCATCATCAATTTCATTTTCAATAAAATTGATTGTATTGTATTATGTGACAGGATTTGAAGGCCCAACTTGATAACGGAAACAAGTTACCTTTCCCTGATGGAATTCTTATCAATGGTCGTGGGTCTGGAGGATCATCTCTCAATGTGGAGCAAGGTACTGCATTACCTACATGAATAAGACTCATATAATGTTTCATTTGGCTTTCCTCAACATTAGTACTAAATTTGACACAACTAATATTTTTTGGCCCTTGTCATTGATGTTACAGGAAAGACTTACAGGCTTAGAATATCAAATGTGGGATTGGAAAATTCACTCAACTTTCGCATACAGAATCACAAGATGAAGCTGGTAGAAGTGGAAGGAACACACACCCTCCAAACCACCTACTCCTCCCTTGACGTTCACGTGGGTCAATCATACTCAGTTCTAGTAACCGCGGATCAACCTGGTCAAGACTATTATATTGTTGTTTCCTCTCGATTTACCAATCCTGTACTCACCTCCACCGGAATTCTTCGCTACAGCAACTCTGCAGGTCCAGTTTCAGGTCCACCCCCTGGTGGACCCACCATCCAAATTGATTGGTCCTTGAACCAAGCCCGCTCCATTAGGACAAACCTGACAGCAAGTGGACCAAGACCAAACCCGCAAGGATCATACCATTATGGCATGATAAACTTAACGAGGACAATTGTGCTATCAAGTTCAGCTGGACAAGTTAATGGGAAACAAAGATACGCAATTAACAGTGTGTCTTATGTTGCCCCCGATACTCCTCTTAAGCTGGCTGACTTCTTCAAGATAGCAGGTGTTTACCGTCCTGGAAGCATCTCTGATAGACCTAGTGGTGGTGGTATTTATCTCGACACTTCTGTTATGCAAACTGACTATAGAACATTTGTTGAGATTGTTTTCCAAAATGATGAAGACATCCTTCAGAGCTATCATCTTGATGGCTACTCTTTCTTTGTTGTTGGGTGAGTATGATTTTCCATCATATAGATCATTTTCATTTCTTTAAAATCAGTCTAATCTATATATGAATGTTGCATGCAGTATGGATGGAGGACAATGGACAAGTGATAAAAGGAACCAGTACAATCTTAGAGACGCAGTTTCGCGTTCCACCACTCAGGTATATCCAAAGTCATGGACTGCTATCTATGTTGCACTTGACAATGTAGGAATGTGGAACTTGAGGTCAGAATTTTGGGCACGACAATATCTCGGGCAACAGTTTTATATGCGTGTATATACAACATCAACATCACTCAGAGATGAATACCCTATTCCTAAGAATGCATTACTTTGTGGTAGGGCAACTGGAAGACATACTCGACCCCTTTGA
Protein sequence

>MS.gene27107.t1

MALNLVLLCVSVYLFAITTTIVAEDPYRFFNWNVTYGDIYPLGVRQQGILINGQFPGPDIHSVTNDNLIINVFNSLDEPFLISWNGVQQRRNSYEDGVFGTTCPIPPGKNFTYILQVKDQIGSFYYFPSLGFHKAAGGFGGIRILSRPRIPVPFDDPAGDYTVLIGDWYKSNHTDLKAQLDNGNKLPFPDGILINGRGSGGSSLNVEQGKTYRLRISNVGLENSLNFRIQNHKMKLVEVEGTHTLQTTYSSLDVHVGQSYSVLVTADQPGQDYYIVVSSRFTNPVLTSTGILRYSNSAGPVSGPPPGGPTIQIDWSLNQARSIRTNLTASGPRPNPQGSYHYGMINLTRTIVLSSSAGQVNGKQRYAINSVSYVAPDTPLKLADFFKIAGVYRPGSISDRPSGGGIYLDTSVMQTDYRTFVEIVFQNDEDILQSYHLDGYSFFVVGMDGGQWTSDKRNQYNLRDAVSRSTTQVYPKSWTAIYVALDNVGMWNLRSEFWARQYLGQQFYMRVYTTSTSLRDEYPIPKNALLCGRATGRHTRPL