AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27841

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27841.t1 MTR_8g087430 99.070 215 2 0 1 215 1 215 3.30e-157 434
MS.gene27841.t1 MTR_7g056337 73.023 215 58 0 1 215 1 215 5.52e-113 322
MS.gene27841.t1 MTR_4g075550 35.526 228 128 9 2 215 47 269 1.85e-27 105
MS.gene27841.t1 MTR_8g012595 35.088 228 130 7 2 214 47 271 2.57e-27 105
MS.gene27841.t1 MTR_3g113920 33.188 229 132 8 2 215 47 269 2.19e-26 102
MS.gene27841.t1 MTR_8g012605 35.465 172 103 4 2 169 47 214 4.24e-25 99.4
MS.gene27841.t1 MTR_8g012615 33.668 199 115 7 31 215 9 204 2.59e-17 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene27841.t1 AT1G29330 81.860 215 39 0 1 215 1 215 9.28e-134 374
MS.gene27841.t1 AT3G25040 72.093 215 60 0 1 215 1 215 3.43e-116 330
MS.gene27841.t1 AT1G29330 84.314 153 24 0 63 215 1 153 2.30e-94 272
MS.gene27841.t1 AT3G25160 33.921 227 133 5 2 214 48 271 1.38e-28 108
MS.gene27841.t1 AT1G19970 34.498 229 129 9 2 215 47 269 2.57e-26 102
MS.gene27841.t1 AT4G38790 33.624 229 131 9 2 215 48 270 1.48e-25 100
MS.gene27841.t1 AT2G21190 31.878 229 135 9 2 215 44 266 1.60e-25 100
MS.gene27841.t1 AT1G75760 32.751 229 133 8 2 215 47 269 1.92e-25 100
MS.gene27841.t1 AT1G75760 34.112 214 121 8 2 201 47 254 6.74e-25 98.6
MS.gene27841.t1 AT1G19970 35.028 177 104 6 2 173 47 217 2.90e-22 91.3
MS.gene27841.t1 AT1G75760 33.714 175 105 6 2 171 47 215 6.63e-21 87.4

Find 36 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGTCAATTTCAGATCTTC+TGG 0.214649 4.3:+13461888 MS.gene27841:intron
GGGCAATATGTATTCTTTCT+TGG 0.265304 4.3:+13461995 MS.gene27841:CDS
TGTGTTCTTAGCACGTTATT+TGG 0.272313 4.3:+13458864 MS.gene27841:CDS
TGTTGCTGCTAGCTTTCTTC+TGG 0.281140 4.3:+13459038 MS.gene27841:CDS
GGCAATATGTATTCTTTCTT+GGG 0.306579 4.3:+13461996 MS.gene27841:CDS
TCTTCTACTATTACTTCATC+AGG 0.375089 4.3:+13462582 MS.gene27841:CDS
GTGGATTATACATTCTCAAT+TGG 0.399809 4.3:+13462386 MS.gene27841:CDS
TACGCTACCAAATCATGTTC+AGG 0.407698 4.3:+13458722 MS.gene27841:CDS
ATTGTGACCTGAACATGATT+TGG 0.429861 4.3:-13458729 None:intergenic
CTTCATAAACGTATTATAAA+CGG 0.434373 4.3:-13458908 None:intergenic
CTAGTTCGTTGGCTATTGTT+TGG 0.468529 4.3:+13458944 MS.gene27841:CDS
ACGTTATTTGGATCTGTTCA+CGG 0.477076 4.3:+13458876 MS.gene27841:CDS
ATGTCAATTTCAGATCTTCT+GGG 0.481234 4.3:+13461889 MS.gene27841:intron
GGAAATGTTGATAATTTGAC+TGG 0.481668 4.3:+13461974 MS.gene27841:CDS
GGCGAGTCTGAAGATGTTCA+TGG 0.501045 4.3:-13458651 None:intergenic
TTCGTTGCAACAAAACCAGC+TGG 0.501585 4.3:-13461949 None:intergenic
GTGCATGCGTGTTCATCCTA+TGG 0.502901 4.3:+13458966 MS.gene27841:CDS
TCATAGCTTCTCTTAACCAT+AGG 0.504328 4.3:-13458982 None:intergenic
CTGAACAACACCTGAGACAC+AGG 0.506289 4.3:-13462540 None:intergenic
CTGAGCCACGTTTCACTCGA+TGG 0.510830 4.3:+13462425 MS.gene27841:CDS
AGTTGCAATACTGCCCCAGC+TGG 0.517340 4.3:+13461934 MS.gene27841:CDS
AGTCTTCATTGCTAGTTCGT+TGG 0.524045 4.3:+13458933 MS.gene27841:CDS
CCACGTTTCACTCGATGGAT+AGG 0.526685 4.3:+13462430 MS.gene27841:CDS
GATAATGACGAAAAGTATCA+AGG 0.541133 4.3:-13459011 None:intergenic
AATCTTGAGATAATTCAAGC+AGG 0.588695 4.3:-13462715 None:intergenic
TTAAATTGCAGTGCGTATCG+TGG 0.598999 4.3:+13462367 MS.gene27841:intron
CGAGAAATTCACCTTCCTTG+AGG 0.604093 4.3:+13459074 MS.gene27841:CDS
TCGTTGCAACAAAACCAGCT+GGG 0.607236 4.3:-13461948 None:intergenic
ATGAACATCTTCAGACTCGC+CGG 0.613512 4.3:+13458653 MS.gene27841:CDS
ATAGAAAGGGACCTCAAGGA+AGG 0.624693 4.3:-13459085 None:intergenic
GAGGAGATGAGTCATGTCAC+CGG 0.626170 4.3:-13458672 None:intergenic
CCTATCCATCGAGTGAAACG+TGG 0.656144 4.3:-13462430 None:intergenic
CGTTGCAACAAAACCAGCTG+GGG 0.659984 4.3:-13461947 None:intergenic
GGAGGAGAACGAGAATGCTG+AGG 0.684833 4.3:-13458691 None:intergenic
AACGTGGCTCAGTCAAATAG+CGG 0.705507 4.3:-13462414 None:intergenic
GAAATGTTGATAATTTGACT+GGG 0.715465 4.3:+13461975 MS.gene27841:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTAGTATAATTTGAA+AGG + chr4.3:13461420-13461439 MS.gene27841:intron 10.0%
!! AATAAGGCTATAAAATAAAA+AGG + chr4.3:13459721-13459740 MS.gene27841:intron 15.0%
!! GATAATTATTAAAAGAAACA+TGG + chr4.3:13459810-13459829 MS.gene27841:intron 15.0%
!!! ATTTGAAAATGTTGTTTATT+TGG + chr4.3:13462177-13462196 MS.gene27841:intron 15.0%
!!! TTGATAATTGATATATGTAA+TGG + chr4.3:13462146-13462165 MS.gene27841:intron 15.0%
!! AGGTAAACAATATTTATTTG+TGG + chr4.3:13462602-13462621 MS.gene27841:intron 20.0%
!! ATAAGATTCATAGATAGAAA+GGG - chr4.3:13459101-13459120 None:intergenic 20.0%
!! ATTACAGTATGAGTAAAATA+AGG + chr4.3:13459705-13459724 MS.gene27841:intron 20.0%
!! ATTGTATGAAAGTAAACAAT+TGG - chr4.3:13459346-13459365 None:intergenic 20.0%
!! CACTGCAATTTAAAAATAAA+TGG - chr4.3:13462360-13462379 None:intergenic 20.0%
!! CTTCATAAACGTATTATAAA+CGG - chr4.3:13458911-13458930 None:intergenic 20.0%
!! GAAACTAACAAAAAAAAACA+GGG - chr4.3:13460976-13460995 None:intergenic 20.0%
!! GCATTAAATAGTTAGAAAAA+GGG - chr4.3:13459907-13459926 None:intergenic 20.0%
!! TGAAACTAACAAAAAAAAAC+AGG - chr4.3:13460977-13460996 None:intergenic 20.0%
!! TGAACAAATCAACTTAATTT+AGG - chr4.3:13460516-13460535 None:intergenic 20.0%
!! TGAATAAATAAAACAGAAAC+TGG - chr4.3:13460181-13460200 None:intergenic 20.0%
!! TGCATTAAATAGTTAGAAAA+AGG - chr4.3:13459908-13459927 None:intergenic 20.0%
!! TTAAAAAAATTCAAACAGGT+CGG - chr4.3:13460118-13460137 None:intergenic 20.0%
!! TTTAGTATAATTTGAAAGGA+TGG + chr4.3:13461424-13461443 MS.gene27841:intron 20.0%
!!! AGAACAAGTTTTTAATCAAA+TGG + chr4.3:13461395-13461414 MS.gene27841:intron 20.0%
!!! ATTTTTTTCTCTTCAATTTC+AGG + chr4.3:13458808-13458827 MS.gene27841:intron 20.0%
!!! GTTTTTTTTTGTTAGTTTCA+TGG + chr4.3:13460977-13460996 MS.gene27841:intron 20.0%
!!! TTAGTTCTATAAACCAATTT+TGG - chr4.3:13460950-13460969 None:intergenic 20.0%
!!! TTTTTATCGCAATTTCAAAA+GGG - chr4.3:13460874-13460893 None:intergenic 20.0%
!!! TTTTTTATCGCAATTTCAAA+AGG - chr4.3:13460875-13460894 None:intergenic 20.0%
! AAGATTTGAAAACAATTGAC+AGG - chr4.3:13461723-13461742 None:intergenic 25.0%
! AGAGAAAATACATTTCTTAG+AGG - chr4.3:13462124-13462143 None:intergenic 25.0%
! ATAAAGAAATGACTTTACAG+TGG - chr4.3:13461244-13461263 None:intergenic 25.0%
! ATAAAGCAGTCAAAACATAA+AGG - chr4.3:13462063-13462082 None:intergenic 25.0%
! ATATCTTTGTCCAAAATGTT+TGG - chr4.3:13461473-13461492 None:intergenic 25.0%
! ATATGATGATATGATCTCTT+GGG + chr4.3:13459578-13459597 MS.gene27841:intron 25.0%
! ATGAAATGAAACTTATCCAT+AGG - chr4.3:13461097-13461116 None:intergenic 25.0%
! CATAAGATTCATAGATAGAA+AGG - chr4.3:13459102-13459121 None:intergenic 25.0%
! GACTTAAAGTCATAACTATA+AGG - chr4.3:13460585-13460604 None:intergenic 25.0%
! TAAAAAAATTCAAACAGGTC+GGG - chr4.3:13460117-13460136 None:intergenic 25.0%
! TAAATAACCAATAACAGCAA+CGG - chr4.3:13460487-13460506 None:intergenic 25.0%
! TATATGATGATATGATCTCT+TGG + chr4.3:13459577-13459596 MS.gene27841:intron 25.0%
! TATGCATAAAAATCAACACT+CGG - chr4.3:13460662-13460681 None:intergenic 25.0%
! TATGCATTTGAAGGAATTAA+AGG - chr4.3:13461062-13461081 None:intergenic 25.0%
! TTAAAGTCATAACTATAAGG+TGG - chr4.3:13460582-13460601 None:intergenic 25.0%
! TTAGATGATTATGCATTTGA+AGG - chr4.3:13461071-13461090 None:intergenic 25.0%
! TTTACTAGTAATGACAAAGT+TGG + chr4.3:13461564-13461583 MS.gene27841:intron 25.0%
!! TTTTACTCATACTGTAATGT+TGG - chr4.3:13459703-13459722 None:intergenic 25.0%
!!! ACATTTCTAGATGATTGTTT+TGG + chr4.3:13459439-13459458 MS.gene27841:intron 25.0%
!!! GAAATGTTGATAATTTGACT+GGG + chr4.3:13461975-13461994 MS.gene27841:CDS 25.0%
!!! GAATTTTTTTAAAGCCTTTG+TGG + chr4.3:13460126-13460145 MS.gene27841:intron 25.0%
!!! GGCTTTAAAAAAATTCAAAC+AGG - chr4.3:13460122-13460141 None:intergenic 25.0%
!!! GTTGTATTTGCTATGATATA+TGG + chr4.3:13459414-13459433 MS.gene27841:intron 25.0%
!!! TGTGTTTTTGTTTTGAATGT+TGG + chr4.3:13460917-13460936 MS.gene27841:intron 25.0%
!!! TGTTTTTATTGTTTGGACTT+TGG + chr4.3:13459548-13459567 MS.gene27841:intron 25.0%
!!! TTTTTTGTTAGTTTCATGGT+TGG + chr4.3:13460981-13461000 MS.gene27841:intron 25.0%
AATCAGAATATCTCAAGAGT+AGG - chr4.3:13462502-13462521 None:intergenic 30.0%
AATGACTTTACAGTGGAAAA+AGG - chr4.3:13461237-13461256 None:intergenic 30.0%
ACAATTCATGTTATGAGTTC+AGG + chr4.3:13460713-13460732 MS.gene27841:intron 30.0%
ATAAAACTCATGCATTACCA+AGG - chr4.3:13460755-13460774 None:intergenic 30.0%
ATGACTTTACAGTGGAAAAA+GGG - chr4.3:13461236-13461255 None:intergenic 30.0%
ATGCATAGCTAAAAAAAAGG+AGG - chr4.3:13461682-13461701 None:intergenic 30.0%
ATGTCAATTTCAGATCTTCT+GGG + chr4.3:13461889-13461908 MS.gene27841:intron 30.0%
ATTGCAAACATAGAGAAGTA+GGG + chr4.3:13459639-13459658 MS.gene27841:intron 30.0%
CATAATCATCTAAAAGCCTA+TGG + chr4.3:13461078-13461097 MS.gene27841:intron 30.0%
GAAATAGAAATCATATCACC+AGG - chr4.3:13461619-13461638 None:intergenic 30.0%
GACTTGTGAAATTGAGTTAT+TGG + chr4.3:13460537-13460556 MS.gene27841:intron 30.0%
GGCAAAAAGCAATAAATGTT+GGG - chr4.3:13459886-13459905 None:intergenic 30.0%
GGCAATATGTATTCTTTCTT+GGG + chr4.3:13461996-13462015 MS.gene27841:CDS 30.0%
GTCCAAACAATAAAAACATC+AGG - chr4.3:13459546-13459565 None:intergenic 30.0%
GTGATGCATAGCTAAAAAAA+AGG - chr4.3:13461685-13461704 None:intergenic 30.0%
GTTTACACAGAATCATGATT+GGG + chr4.3:13460011-13460030 MS.gene27841:intron 30.0%
TACTCTTGAGATATTCTGAT+TGG + chr4.3:13462501-13462520 MS.gene27841:intron 30.0%
TATGTCAATTTCAGATCTTC+TGG + chr4.3:13461888-13461907 MS.gene27841:intron 30.0%
TCAATTGTTAGTGAAAGTGA+TGG - chr4.3:13458783-13458802 None:intergenic 30.0%
TCTTCTACTATTACTTCATC+AGG + chr4.3:13462582-13462601 MS.gene27841:CDS 30.0%
TGTTTACACAGAATCATGAT+TGG + chr4.3:13460010-13460029 MS.gene27841:intron 30.0%
! AACAATTGACAGGTTTTCTA+TGG - chr4.3:13461713-13461732 None:intergenic 30.0%
! AGTGTTTAAGCCAAACATTT+TGG + chr4.3:13461460-13461479 MS.gene27841:intron 30.0%
! ATAGCAAAGCATTCCAAAAT+AGG - chr4.3:13460790-13460809 None:intergenic 30.0%
! GTGGATTATACATTCTCAAT+TGG + chr4.3:13462386-13462405 MS.gene27841:CDS 30.0%
! GTTTTACGATTTTACTACCT+TGG + chr4.3:13460735-13460754 MS.gene27841:intron 30.0%
! TGTTGTTTATTTGGCTGAAT+TGG + chr4.3:13462186-13462205 MS.gene27841:intron 30.0%
! TTTGTTCAGCTTTCCTATTT+TGG + chr4.3:13460774-13460793 MS.gene27841:intron 30.0%
!! AAAGGATTAGCTGAAAAAAC+AGG + chr4.3:13459739-13459758 MS.gene27841:intron 30.0%
!! GATAATGACGAAAAGTATCA+AGG - chr4.3:13459014-13459033 None:intergenic 30.0%
!! GGAAATGTTGATAATTTGAC+TGG + chr4.3:13461974-13461993 MS.gene27841:CDS 30.0%
!! GTCCTGATGTTTTTATTGTT+TGG + chr4.3:13459541-13459560 MS.gene27841:intron 30.0%
!! TTAGTGAAAGTGATGGATTA+GGG - chr4.3:13458776-13458795 None:intergenic 30.0%
!!! AGATTCAGTTTTGAGATGAA+GGG - chr4.3:13460240-13460259 None:intergenic 30.0%
!!! AGGATTTTGGTTGTAAAAAC+AGG - chr4.3:13460398-13460417 None:intergenic 30.0%
!!! GGATTTTGGTTGTAAAAACA+GGG - chr4.3:13460397-13460416 None:intergenic 30.0%
AAAACAAGCAACAACAGAAG+AGG - chr4.3:13460418-13460437 None:intergenic 35.0%
AAATACAACACATCCTCGAA+TGG - chr4.3:13459403-13459422 None:intergenic 35.0%
AAATTCATGCCATCTTCTTC+TGG + chr4.3:13461528-13461547 MS.gene27841:intron 35.0%
AAGCAGTCAAAACATAAAGG+TGG - chr4.3:13462060-13462079 None:intergenic 35.0%
AATAGAATTCAGTACGGACA+GGG - chr4.3:13461767-13461786 None:intergenic 35.0%
ACAAAAATAGAGCACAAGAG+AGG - chr4.3:13460294-13460313 None:intergenic 35.0%
ACATTTCTTAGAGGACAATC+AGG - chr4.3:13462115-13462134 None:intergenic 35.0%
ACGTTATTTGGATCTGTTCA+CGG + chr4.3:13458876-13458895 MS.gene27841:CDS 35.0%
ATTGTGACCTGAACATGATT+TGG - chr4.3:13458732-13458751 None:intergenic 35.0%
CAAAAATAGAGCACAAGAGA+GGG - chr4.3:13460293-13460312 None:intergenic 35.0%
CATTGCAAACATAGAGAAGT+AGG + chr4.3:13459638-13459657 MS.gene27841:intron 35.0%
CTTAAACACTGCATGATACT+TGG - chr4.3:13461450-13461469 None:intergenic 35.0%
GCACGTAATAGAATTCAGTA+CGG - chr4.3:13461773-13461792 None:intergenic 35.0%
GGGCAAAAAGCAATAAATGT+TGG - chr4.3:13459887-13459906 None:intergenic 35.0%
GGGCAATATGTATTCTTTCT+TGG + chr4.3:13461995-13462014 MS.gene27841:CDS 35.0%
GTGGGATAAAATGAATTTCC+TGG + chr4.3:13461598-13461617 MS.gene27841:intron 35.0%
TAATAGAATTCAGTACGGAC+AGG - chr4.3:13461768-13461787 None:intergenic 35.0%
TACTGAAATCGGAAAGAACA+TGG - chr4.3:13460082-13460101 None:intergenic 35.0%
TAGGGATTGTGAAATGTGAA+GGG - chr4.3:13458758-13458777 None:intergenic 35.0%
TCATAGCTTCTCTTAACCAT+AGG - chr4.3:13458985-13459004 None:intergenic 35.0%
TCTTTCCGATTTCAGTAACT+GGG + chr4.3:13460085-13460104 MS.gene27841:intron 35.0%
TGTGTTCTTAGCACGTTATT+TGG + chr4.3:13458864-13458883 MS.gene27841:CDS 35.0%
TTAGGGATTGTGAAATGTGA+AGG - chr4.3:13458759-13458778 None:intergenic 35.0%
TTCTATTTCCTTCTTCCGTA+TGG + chr4.3:13461630-13461649 MS.gene27841:intron 35.0%
TTCTTTCCGATTTCAGTAAC+TGG + chr4.3:13460084-13460103 MS.gene27841:intron 35.0%
TTGCAAACATAGAGAAGTAG+GGG + chr4.3:13459640-13459659 MS.gene27841:intron 35.0%
! CTGGGCATTTTCAATATACT+TGG + chr4.3:13461907-13461926 MS.gene27841:CDS 35.0%
! GGCATTTTCAATATACTTGG+AGG + chr4.3:13461910-13461929 MS.gene27841:CDS 35.0%
! TTTTACCAGTGTCAAACTGT+CGG - chr4.3:13459208-13459227 None:intergenic 35.0%
!! GTTAGTGAAAGTGATGGATT+AGG - chr4.3:13458777-13458796 None:intergenic 35.0%
!!! AAGGGAATTGGTTTTTAGCA+AGG - chr4.3:13460222-13460241 None:intergenic 35.0%
!!! AAGTGTTGGTGCTACATTTT+CGG + chr4.3:13459297-13459316 MS.gene27841:intron 35.0%
!!! AGGGAATTGGTTTTTAGCAA+GGG - chr4.3:13460221-13460240 None:intergenic 35.0%
!!! AGTTTTGAGATGAAGGGAAT+TGG - chr4.3:13460234-13460253 None:intergenic 35.0%
!!! ATTTGGTAGCGTAGATTTTG+AGG - chr4.3:13458715-13458734 None:intergenic 35.0%
!!! CAGATTCAGTTTTGAGATGA+AGG - chr4.3:13460241-13460260 None:intergenic 35.0%
!!! CAGGAATTTTGTTTTGCAGT+TGG + chr4.3:13462668-13462687 MS.gene27841:intron 35.0%
!!! CTGTTCTTTTCTAGAGTTCT+TGG - chr4.3:13461292-13461311 None:intergenic 35.0%
!!! TTCTAGATGATTGTTTTGGC+TGG + chr4.3:13459443-13459462 MS.gene27841:intron 35.0%
ACGGACAGGGAATATATTCT+TGG - chr4.3:13461754-13461773 None:intergenic 40.0%
AGGGATTGTGAAATGTGAAG+GGG - chr4.3:13458757-13458776 None:intergenic 40.0%
AGTCTTCATTGCTAGTTCGT+TGG + chr4.3:13458933-13458952 MS.gene27841:CDS 40.0%
ATAGATAGAAAGGGACCTCA+AGG - chr4.3:13459092-13459111 None:intergenic 40.0%
ATTTGAGTGACGCTTAACCT+AGG + chr4.3:13459497-13459516 MS.gene27841:intron 40.0%
CAGGGAATATATTCTTGGAC+AGG - chr4.3:13461749-13461768 None:intergenic 40.0%
CTTCTCTATGTTTGCAATGC+AGG - chr4.3:13459637-13459656 None:intergenic 40.0%
GTAAAAACAGGGTACAAGAG+AGG - chr4.3:13460386-13460405 None:intergenic 40.0%
TACGCTACCAAATCATGTTC+AGG + chr4.3:13458722-13458741 MS.gene27841:CDS 40.0%
TCGTACAAGTCTACGATTTC+TGG + chr4.3:13459970-13459989 MS.gene27841:intron 40.0%
TCTGTCCCAGTTACTGAAAT+CGG - chr4.3:13460093-13460112 None:intergenic 40.0%
TTAAATTGCAGTGCGTATCG+TGG + chr4.3:13462367-13462386 MS.gene27841:intron 40.0%
TTTCATGGTTGGAGAGGAAT+GGG + chr4.3:13460992-13461011 MS.gene27841:intron 40.0%
TTTCGCAGATGCAAATGAGA+CGG - chr4.3:13459379-13459398 None:intergenic 40.0%
TTTGAGTGACGCTTAACCTA+GGG + chr4.3:13459498-13459517 MS.gene27841:intron 40.0%
! ATTTTATCCCACACTGTGAG+CGG - chr4.3:13461590-13461609 None:intergenic 40.0%
! CTAGTTCGTTGGCTATTGTT+TGG + chr4.3:13458944-13458963 MS.gene27841:CDS 40.0%
! GCAACAACAGAAGAGGATTT+TGG - chr4.3:13460411-13460430 None:intergenic 40.0%
! TCTAACTCCGTTGCTGTTAT+TGG + chr4.3:13460477-13460496 MS.gene27841:intron 40.0%
! TGTTAGTTTCATGGTTGGAG+AGG + chr4.3:13460986-13461005 MS.gene27841:intron 40.0%
! TGTTGGAGCATCTCCAAAAT+TGG + chr4.3:13460934-13460953 MS.gene27841:intron 40.0%
!! TCTTGGGCATTTTGTTTCGA+GGG + chr4.3:13459594-13459613 MS.gene27841:intron 40.0%
!!! AGTGTTGGTGCTACATTTTC+GGG + chr4.3:13459298-13459317 MS.gene27841:intron 40.0%
AACGTGGCTCAGTCAAATAG+CGG - chr4.3:13462417-13462436 None:intergenic 45.0%
AATCCATAACCCTGTTCCCT+AGG - chr4.3:13459517-13459536 None:intergenic 45.0%
AGCAGATTCCATACGGAAGA+AGG - chr4.3:13461641-13461660 None:intergenic 45.0%
ATAACCCTAGCCACCATTGT+TGG - chr4.3:13459669-13459688 None:intergenic 45.0%
ATAGAAAGGGACCTCAAGGA+AGG - chr4.3:13459088-13459107 None:intergenic 45.0%
ATGAACATCTTCAGACTCGC+CGG + chr4.3:13458653-13458672 MS.gene27841:CDS 45.0%
CGAGAAATTCACCTTCCTTG+AGG + chr4.3:13459074-13459093 MS.gene27841:CDS 45.0%
GAAGTAGGGGTAACCAACAA+TGG + chr4.3:13459653-13459672 MS.gene27841:intron 45.0%
GAATTGCAGCCAGAAGAAGA+TGG - chr4.3:13461540-13461559 None:intergenic 45.0%
GGAGACTGAACACTGTGATT+CGG + chr4.3:13461017-13461036 MS.gene27841:intron 45.0%
GTAACCAACAATGGTGGCTA+GGG + chr4.3:13459662-13459681 MS.gene27841:intron 45.0%
GTACAAGAGAGGATGTGTTC+TGG - chr4.3:13460375-13460394 None:intergenic 45.0%
GTTTCATGGTTGGAGAGGAA+TGG + chr4.3:13460991-13461010 MS.gene27841:intron 45.0%
TAACCTAGGGAACAGGGTTA+TGG + chr4.3:13459511-13459530 MS.gene27841:intron 45.0%
TAACGAATGCTGCGTTGAAG+TGG + chr4.3:13461839-13461858 MS.gene27841:intron 45.0%
TCGTTGCAACAAAACCAGCT+GGG - chr4.3:13461951-13461970 None:intergenic 45.0%
TGTAGCACCAACACTTCTGA+TGG - chr4.3:13459293-13459312 None:intergenic 45.0%
TTCGTTGCAACAAAACCAGC+TGG - chr4.3:13461952-13461971 None:intergenic 45.0%
! CTTCTTCTGGCTGCAATTCA+TGG + chr4.3:13461541-13461560 MS.gene27841:intron 45.0%
!! ACTGGTCTTATTGCCAAACC+AGG - chr4.3:13460163-13460182 None:intergenic 45.0%
!! CTCTTGGGCATTTTGTTTCG+AGG + chr4.3:13459593-13459612 MS.gene27841:intron 45.0%
!! TCTACGATTTCTGGAGTGCT+TGG + chr4.3:13459979-13459998 MS.gene27841:intron 45.0%
!! TGGTAGCGTAGATTTTGAGG+AGG - chr4.3:13458712-13458731 None:intergenic 45.0%
!! TGTTGCTGCTAGCTTTCTTC+TGG + chr4.3:13459038-13459057 MS.gene27841:CDS 45.0%
!!! CTGGTTTTGTTGCAACGAAG+TGG + chr4.3:13461953-13461972 MS.gene27841:CDS 45.0%
AAGATCGCGTGAAATCGCTG+TGG + chr4.3:13460035-13460054 MS.gene27841:intron 50.0%
AGGTCAGAGTTCAGCCACAA+AGG - chr4.3:13460143-13460162 None:intergenic 50.0%
ATGGTTGGAGAGGAATGGGT+TGG + chr4.3:13460996-13461015 MS.gene27841:intron 50.0%
CAGCTGCAGCAGATTCCATA+CGG - chr4.3:13461648-13461667 None:intergenic 50.0%
CCACGTTTCACTCGATGGAT+AGG + chr4.3:13462430-13462449 MS.gene27841:CDS 50.0%
CCTATCCATCGAGTGAAACG+TGG - chr4.3:13462433-13462452 None:intergenic 50.0%
CGCGTAGAAGCAGAAGATGT+TGG - chr4.3:13460340-13460359 None:intergenic 50.0%
CGTTGCAACAAAACCAGCTG+GGG - chr4.3:13461950-13461969 None:intergenic 50.0%
CTGAACAACACCTGAGACAC+AGG - chr4.3:13462543-13462562 None:intergenic 50.0%
GACGCTTAACCTAGGGAACA+GGG + chr4.3:13459505-13459524 MS.gene27841:intron 50.0%
GAGGAGATGAGTCATGTCAC+CGG - chr4.3:13458675-13458694 None:intergenic 50.0%
GGTAACCAACAATGGTGGCT+AGG + chr4.3:13459661-13459680 MS.gene27841:intron 50.0%
GTAGGGGTAACCAACAATGG+TGG + chr4.3:13459656-13459675 MS.gene27841:intron 50.0%
GTGCATGCGTGTTCATCCTA+TGG + chr4.3:13458966-13458985 MS.gene27841:CDS 50.0%
TAGCAAGGGAAACGACGCAA+CGG - chr4.3:13460207-13460226 None:intergenic 50.0%
TGACACCGACAGTTTGACAC+TGG + chr4.3:13459200-13459219 MS.gene27841:intron 50.0%
TGACGCTTAACCTAGGGAAC+AGG + chr4.3:13459504-13459523 MS.gene27841:intron 50.0%
TTTGTGGCTGAACTCTGACC+TGG + chr4.3:13460142-13460161 MS.gene27841:intron 50.0%
! CACGCATCCATCAGAAGTGT+TGG + chr4.3:13459283-13459302 MS.gene27841:intron 50.0%
! CACTTCTGATGGATGCGTGT+TGG - chr4.3:13459282-13459301 None:intergenic 50.0%
! GTTTTTGCAGCCTGTGTCTC+AGG + chr4.3:13462530-13462549 MS.gene27841:intron 50.0%
AAAGTTGGCCGCTCACAGTG+TGG + chr4.3:13461579-13461598 MS.gene27841:intron 55.0%
AAGTTGGCCGCTCACAGTGT+GGG + chr4.3:13461580-13461599 MS.gene27841:intron 55.0%
AGCAAGGGAAACGACGCAAC+GGG - chr4.3:13460206-13460225 None:intergenic 55.0%
AGTTGCAATACTGCCCCAGC+TGG + chr4.3:13461934-13461953 MS.gene27841:CDS 55.0%
CTGAGCCACGTTTCACTCGA+TGG + chr4.3:13462425-13462444 MS.gene27841:CDS 55.0%
GCATCTGCGAAAGCCATTCG+AGG + chr4.3:13459387-13459406 MS.gene27841:intron 55.0%
GGAGGAGAACGAGAATGCTG+AGG - chr4.3:13458694-13458713 None:intergenic 55.0%
GGCTGAACTCTGACCTGGTT+TGG + chr4.3:13460147-13460166 MS.gene27841:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 13458653 13462723 13458653 ID=MS.gene27841
chr4.3 mRNA 13458653 13462723 13458653 ID=MS.gene27841.t1;Parent=MS.gene27841
chr4.3 exon 13458653 13458743 13458653 ID=MS.gene27841.t1.exon1;Parent=MS.gene27841.t1
chr4.3 CDS 13458653 13458743 13458653 ID=cds.MS.gene27841.t1;Parent=MS.gene27841.t1
chr4.3 exon 13458830 13459095 13458830 ID=MS.gene27841.t1.exon2;Parent=MS.gene27841.t1
chr4.3 CDS 13458830 13459095 13458830 ID=cds.MS.gene27841.t1;Parent=MS.gene27841.t1
chr4.3 exon 13461902 13462017 13461902 ID=MS.gene27841.t1.exon3;Parent=MS.gene27841.t1
chr4.3 CDS 13461902 13462017 13461902 ID=cds.MS.gene27841.t1;Parent=MS.gene27841.t1
chr4.3 exon 13462378 13462451 13462378 ID=MS.gene27841.t1.exon4;Parent=MS.gene27841.t1
chr4.3 CDS 13462378 13462451 13462378 ID=cds.MS.gene27841.t1;Parent=MS.gene27841.t1
chr4.3 exon 13462540 13462603 13462540 ID=MS.gene27841.t1.exon5;Parent=MS.gene27841.t1
chr4.3 CDS 13462540 13462603 13462540 ID=cds.MS.gene27841.t1;Parent=MS.gene27841.t1
chr4.3 exon 13462687 13462723 13462687 ID=MS.gene27841.t1.exon6;Parent=MS.gene27841.t1
chr4.3 CDS 13462687 13462723 13462687 ID=cds.MS.gene27841.t1;Parent=MS.gene27841.t1
Gene Sequence

>MS.gene27841

ATGAACATCTTCAGACTCGCCGGTGACATGACTCATCTCCTCAGCATTCTCGTTCTCCTCCTCAAAATCTACGCTACCAAATCATGTTCAGGTCACAATTTCCCCTTCACATTTCACAATCCCTAATCCATCACTTTCACTAACAATTGATCATGATTTTTTTCTCTTCAATTTCAGGTGTTTCTCGTAAGACTCAAGAACTATACGCGATTGTGTTCTTAGCACGTTATTTGGATCTGTTCACGGATTTCATTTCCGTTTATAATACGTTTATGAAGATAGTCTTCATTGCTAGTTCGTTGGCTATTGTTTGGTGCATGCGTGTTCATCCTATGGTTAAGAGAAGCTATGATAAAGACCTTGATACTTTTCGTCATTATCTTCTTGTTGCTGCTAGCTTTCTTCTGGCACTCGTCTTGCACGAGAAATTCACCTTCCTTGAGGTCCCTTTCTATCTATGAATCTTATGTTTCTCTTTTTTAAGTATTTTGCAATGTTAATGTTTGAGTTGCGTGATTTAATTGTGTTTTATTTGTTTATTTTACACTGACACCGACAGTTTGACACTGGTAAAAATTTGATTAATTGAATTTTCTCACATGTCAGTGTCTATGATGTATCTGACACCAACACGCATCCATCAGAAGTGTTGGTGCTACATTTTCGGGTTTTTAATCGTTGAGCATTTCTCCAATTGTTTACTTTCATACAATTTTTATGTTTCCGTCTCATTTGCATCTGCGAAAGCCATTCGAGGATGTGTTGTATTTGCTATGATATATGGATACATTTCTAGATGATTGTTTTGGCTGGTGATGACAAACTTTAGATAAGCAAGAAACATATTTGAGTGACGCTTAACCTAGGGAACAGGGTTATGGATTTTTTGTCCTGATGTTTTTATTGTTTGGACTTTGGAGTTCTTATATGATGATATGATCTCTTGGGCATTTTGTTTCGAGGGATGATTCTTTTGTTAAACCTGCATTGCAAACATAGAGAAGTAGGGGTAACCAACAATGGTGGCTAGGGTTATATTTGCATCAACCAACATTACAGTATGAGTAAAATAAGGCTATAAAATAAAAAGGATTAGCTGAAAAAACAGGAAAAAACAATCAGTTTATTTCATTTTCATTTTTTGTCTATGTTTCATCGATAATTATTAAAAGAAACATGGTATTTTTTTTAATTATCTATAAAAACAAATCAACTGAAAATCAAACGCAACCCAACATTTATTGCTTTTTGCCCTTTTTCTAACTATTTAATGCACATAGTTATTGAAATCTCGATTTAAAACGAAAAATCGAAAAATCGTACAAGTCTACGATTTCTGGAGTGCTTGGTGATTTCATGTTTACACAGAATCATGATTGGGTAAGATCGCGTGAAATCGCTGTGGAAAAACATAAAGTTGTGCAATCCATGTTCTTTCCGATTTCAGTAACTGGGACAGATCCCGACCTGTTTGAATTTTTTTAAAGCCTTTGTGGCTGAACTCTGACCTGGTTTGGCAATAAGACCAGTTTCTGTTTTATTTATTCATACCCGTTGCGTCGTTTCCCTTGCTAAAAACCAATTCCCTTCATCTCAAAACTGAATCTGTAATGCTTTGTTTTTGTGAGCATAACTCACCCTCTCTTGTGCTCTATTTTTGTGTTCATGTGCTTTGTTTTTGCGACCAACATCTTCTGCTTCTACGCGCTGTTTTTGCAACCAGAACACATCCTCTCTTGTACCCTGTTTTTACAACCAAAATCCTCTTCTGTTGTTGCTTGTTTTTGCTGCTAAGTTCTGTTCTTAATTCGATTCATTAGAATGTCTAACTCCGTTGCTGTTATTGGTTATTTAAGCAAGCCTAAATTAAGTTGATTTGTTCATGACTTGTGAAATTGAGTTATTGGTTATTTGAGAACTTATTTACCACCTTATAGTTATGACTTTAAGTCTAATTAAGTATTTGAATCTGAAGACTTACTTTCACTTACTAATGTTAATTTATGCCGAGTGTTGATTTTTATGCATATTATATGAGTTTAATGAATTTGTAGAATCTTACAATTCATGTTATGAGTTCAGGTTTTACGATTTTACTACCTTGGTAATGCATGAGTTTTATTTGTTCAGCTTTCCTATTTTGGAATGCTTTGCTATGCAATTACATTTTTCTCTTTGAGATCAAATGCAAATATAAGTAAATCTTGACAAATTTCATCCCTTTTGAAATTGCGATAAAAAATGCGATGTTTCGAAAAATAATCTGTGTTTTTGTTTTGAATGTTGGAGCATCTCCAAAATTGGTTTATAGAACTAAAAACCCTGTTTTTTTTTGTTAGTTTCATGGTTGGAGAGGAATGGGTTGGAGACTGAACACTGTGATTCGGTTTAAGATCTGATTTTTGTCCTTTAATTCCTTCAAATGCATAATCATCTAAAAGCCTATGGATAAGTTTCATTTCATATTTTCATGCAAATTTTGCATATTTGAGCAAAGTTGATAGACATTAATATTCTATCAAATGTGTTGAATCTGACGATATTTCAAAACTTATCTGATCACTTGTGACATTCTTTAATCCCTTTTTCCACTGTAAAGTCATTTCTTTATATGTTTGAACGTTGCTCATAGAAAACCAAGAACTCTAGAAAAGAACAGCTTCAGATATGTTGAGTGTTTCTTAATGTCAAGTGTCTCTGAACATCTTGATTATTTGTGTCAAATCTTGTAACATGTTAACAAGAACAAGTTTTTAATCAAATGGTTTTATTTTAGTATAATTTGAAAGGATGGCCAAGTATCATGCAGTGTTTAAGCCAAACATTTTGGACAAAGATATATTTTGTTGATAGTTTGAAATATTTAACATTTATAAAATTCATGCCATCTTCTTCTGGCTGCAATTCATGGTTTACTAGTAATGACAAAGTTGGCCGCTCACAGTGTGGGATAAAATGAATTTCCTGGTGATATGATTTCTATTTCCTTCTTCCGTATGGAATCTGCTGCAGCTGTGATTTATTTTCCTCCTTTTTTTTAGCTATGCATCACAAAAGCCATAGAAAACCTGTCAATTGTTTTCAAATCTTATTCCTGTCCAAGAATATATTCCCTGTCCGTACTGAATTCTATTACGTGCGCTACATGTTAGCTTTATAGTAATTCTGTTACACAATGTAACATAATAACGAATGCTGCGTTGAAGTGGAAATGCTCAATATACTTTGTTTTATGTATGTCAATTTCAGATCTTCTGGGCATTTTCAATATACTTGGAGGCAGTTGCAATACTGCCCCAGCTGGTTTTGTTGCAACGAAGTGGAAATGTTGATAATTTGACTGGGCAATATGTATTCTTTCTTGGGTATGTGCTCTTTTGTTTATTTAGCTTCTCTTTTACGATCCACCTTTATGTTTTGACTGCTTTATACTATGAACTTTCTAGATACTTTTGTCATCCTGATTGTCCTCTAAGAAATGTATTTTCTCTACTTGATAATTGATATATGTAATGGTTGTCATTATTTGAAAATGTTGTTTATTTGGCTGAATTGGCATTTTTCTGCTGACGTGTTGTCTAACAACTTGTCTCTAGTCTTCATGACTGCTTAGTTTCATGAGCAGTTTGCTGTAGAACTTTTAAATATTACTAGACAATCTGATCTTGTTCAAAATCTATTTATTATGATAAGTAAATATCATACCATTTATTTTTAAATTGCAGTGCGTATCGTGGATTATACATTCTCAATTGGATATACCGCTATTTGACTGAGCCACGTTTCACTCGATGGATAGGTAAATATGCTTAAAATCAAAATATGAATTGCTATTTAAACTATATGCCTACTCTTGAGATATTCTGATTGGCTTCTTGTTTTTGCAGCCTGTGTCTCAGGTGTTGTTCAGACAGCTCTGTATGCTGATTTCTTCTACTATTACTTCATCAGGTAAACAATATTTATTTGTGGAAGTTCATGTTTTTATTATACCGATACAACAAATAGGTAACATCAGGAATTTTGTTTTGCAGTTGGAAGAACAATTCAAAACTGAAGCTGCCTGCTTGA
Protein sequence

>MS.gene27841.t1

MNIFRLAGDMTHLLSILVLLLKIYATKSCSGVSRKTQELYAIVFLARYLDLFTDFISVYNTFMKIVFIASSLAIVWCMRVHPMVKRSYDKDLDTFRHYLLVAASFLLALVLHEKFTFLEIFWAFSIYLEAVAILPQLVLLQRSGNVDNLTGQYVFFLGAYRGLYILNWIYRYLTEPRFTRWIACVSGVVQTALYADFFYYYFISWKNNSKLKLPA