AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28291

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28291.t1 MTR_7g072420 41.593 113 63 3 8 119 270 380 2.10e-19 82.4
MS.gene28291.t1 MTR_4g076100 31.624 117 73 1 9 118 140 256 4.57e-18 78.6
MS.gene28291.t1 MTR_4g076100 31.624 117 73 1 9 118 284 400 8.81e-18 78.2
MS.gene28291.t1 MTR_3g465800 37.500 112 67 3 8 118 262 371 1.01e-17 77.8
MS.gene28291.t1 MTR_7g451450 40.816 98 57 1 16 113 274 370 1.63e-17 77.4
MS.gene28291.t1 MTR_3g465800 37.500 112 67 3 8 118 191 300 1.86e-17 77.0
MS.gene28291.t1 MTR_8g091660 40.196 102 59 2 15 115 276 376 1.65e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28291.t1 AT5G46180 83.784 111 18 0 9 119 240 350 2.49e-62 197
MS.gene28291.t1 AT3G22200 35.965 114 66 1 12 118 276 389 8.35e-20 84.0
MS.gene28291.t1 AT3G22200 35.965 114 66 1 12 118 285 398 9.12e-20 83.6
MS.gene28291.t1 AT2G38400 39.286 112 66 2 8 119 267 376 4.51e-19 81.6
MS.gene28291.t1 AT2G38400 39.286 112 66 2 8 119 267 376 4.93e-19 81.6
MS.gene28291.t1 AT1G80600 40.816 98 57 1 16 113 264 360 4.54e-18 79.0
MS.gene28291.t1 AT3G08860 40.179 112 64 3 8 118 270 379 1.21e-17 77.8
MS.gene28291.t1 AT3G08860 40.179 112 64 3 8 118 270 379 1.23e-17 77.8
MS.gene28291.t1 AT4G39660 37.500 112 67 3 9 119 191 300 3.01e-15 70.9
MS.gene28291.t1 AT4G39660 37.500 112 67 3 9 119 266 375 4.14e-15 70.5

Find 25 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCATCAACACGTTATATT+TGG 0.235083 2.1:+64689850 None:intergenic
TCTGCAAGAACTGCGCTAAC+TGG 0.362917 2.1:+64689004 None:intergenic
ACAGCTTTCAAATAACCATC+TGG 0.413613 2.1:+64689887 None:intergenic
TCAGATACTAGGGAAAGCTT+TGG 0.426949 2.1:-64689041 MS.gene28291:intron
CAGATACTAGGGAAAGCTTT+GGG 0.440625 2.1:-64689040 MS.gene28291:CDS
TGAACACCAACCACTACATC+TGG 0.440669 2.1:+64689758 None:intergenic
ATTGCTGATGAAATACAAAC+TGG 0.456192 2.1:-64689830 MS.gene28291:CDS
CAAACTGGATTAGCAAGAAC+AGG 0.479961 2.1:-64689815 MS.gene28291:CDS
CTAGGGAAAGCTTTGGGTGG+AGG 0.491794 2.1:-64689034 MS.gene28291:CDS
TTAAACCTTCCATGCTGTCC+AGG 0.491922 2.1:+64688956 None:intergenic
ATGGCAACTGCACTGGCCAA+TGG 0.511220 2.1:+64688390 None:intergenic
TCTCTCAATGTGCTCTTGCA+TGG 0.514697 2.1:+64690504 None:intergenic
TGAGGCAATGGCAACTGCAC+TGG 0.516585 2.1:+64688383 None:intergenic
CAGCTTTCAAATAACCATCT+GGG 0.525779 2.1:+64689888 None:intergenic
TTCTTTGATCACTTCAAGTG+AGG 0.543995 2.1:+64688365 None:intergenic
GATCACTTCAAGTGAGGCAA+TGG 0.551674 2.1:+64688371 None:intergenic
CGAAGTTCGTCCAGATGTAG+TGG 0.573219 2.1:-64689768 MS.gene28291:intron
TGTATAAAACCTGGACAGCA+TGG 0.573539 2.1:-64688965 MS.gene28291:CDS
TAAAACCTGGACAGCATGGA+AGG 0.583742 2.1:-64688961 MS.gene28291:intron
AGCTTTCAAATAACCATCTG+GGG 0.599511 2.1:+64689889 None:intergenic
GTGATGCTTTGTATAAAACC+TGG 0.600068 2.1:-64688974 MS.gene28291:CDS
GCTTTCAAATAACCATCTGG+GGG 0.649011 2.1:+64689890 None:intergenic
ATACTAGGGAAAGCTTTGGG+TGG 0.669588 2.1:-64689037 MS.gene28291:CDS
AAGAAGAGAGACTTGCTGAG+AGG 0.679464 2.1:-64688347 MS.gene28291:intron
CTTTCAAATAACCATCTGGG+GGG 0.711042 2.1:+64689891 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATTGTTTATTAAAGA+AGG - chr2.1:64689779-64689798 MS.gene28291:CDS 10.0%
!!! ATTTTAATGAATGAAATTAT+CGG + chr2.1:64689757-64689776 None:intergenic 10.0%
!!! TTTTATTTTTAATAAGAAAG+TGG - chr2.1:64690325-64690344 MS.gene28291:intron 10.0%
!! AAACTATTATACTTCATAAA+TGG + chr2.1:64688036-64688055 None:intergenic 15.0%
!! AATAAAAAAAATCAGTTTGA+CGG + chr2.1:64688172-64688191 None:intergenic 15.0%
!! AATATTAACGTTATGAAATA+TGG - chr2.1:64688200-64688219 MS.gene28291:intron 15.0%
!! ATAAGAATAGAAAATAGAAT+TGG + chr2.1:64687915-64687934 None:intergenic 15.0%
!! ATACAGAATAAAAATGAAAA+AGG + chr2.1:64689138-64689157 None:intergenic 15.0%
!! ATATTAACGTTATGAAATAT+GGG - chr2.1:64688201-64688220 MS.gene28291:intron 15.0%
!! TAAAAAAAAATTCATCCTAT+GGG - chr2.1:64689677-64689696 MS.gene28291:intron 15.0%
!! TAAAATAAATAACCATTACA+AGG + chr2.1:64689659-64689678 None:intergenic 15.0%
!! TACAGAATAAAAATGAAAAA+GGG + chr2.1:64689137-64689156 None:intergenic 15.0%
!! TCTGAACTTAAATTATTAAA+AGG + chr2.1:64688645-64688664 None:intergenic 15.0%
!! TTAAAAAAAAATTCATCCTA+TGG - chr2.1:64689676-64689695 MS.gene28291:intron 15.0%
!!! TTTTAATAATTTAAGTTCAG+AGG - chr2.1:64688644-64688663 MS.gene28291:intron 15.0%
!! AAGAAAGTTAAAGAAATTAG+AGG + chr2.1:64689192-64689211 None:intergenic 20.0%
!! AATAGAAAATAGAATTGGTA+AGG + chr2.1:64687910-64687929 None:intergenic 20.0%
!! AGGAAAAACTAGATATAATA+TGG + chr2.1:64688928-64688947 None:intergenic 20.0%
!! ATAGAAAATAGAATTGGTAA+GGG + chr2.1:64687909-64687928 None:intergenic 20.0%
!! GAAAAGTAACAGAAAAAAAA+AGG + chr2.1:64689828-64689847 None:intergenic 20.0%
!! TAAAAGAAATTACGATTTGA+AGG - chr2.1:64690061-64690080 MS.gene28291:intron 20.0%
!! TAACGTCATATAAAAAAATG+TGG + chr2.1:64688326-64688345 None:intergenic 20.0%
!!! AAGAAATTAGAGGTATAAAA+AGG + chr2.1:64689182-64689201 None:intergenic 20.0%
!!! ATGACGTTATATTTTTTTAC+AGG - chr2.1:64688337-64688356 MS.gene28291:intron 20.0%
! AAAACAGAGAATATTATGGT+TGG - chr2.1:64688830-64688849 MS.gene28291:intron 25.0%
! AAGCATTATATGAAAGAATG+GGG + chr2.1:64690439-64690458 None:intergenic 25.0%
! AATCATCAACACGTTATATT+TGG + chr2.1:64688411-64688430 None:intergenic 25.0%
! ACAGAATATATAAGAGGTAA+AGG + chr2.1:64688526-64688545 None:intergenic 25.0%
! AGAGATACAGAATATATAAG+AGG + chr2.1:64688532-64688551 None:intergenic 25.0%
! ATCTCAGAAATTGCTTATAT+AGG - chr2.1:64687832-64687851 MS.gene28291:intron 25.0%
! ATTCTGTTCTTATGAAATGA+TGG - chr2.1:64687797-64687816 MS.gene28291:intron 25.0%
! CAAGCATTATATGAAAGAAT+GGG + chr2.1:64690440-64690459 None:intergenic 25.0%
! CAATAGTTGTCAAACATAAA+AGG - chr2.1:64689372-64689391 MS.gene28291:intron 25.0%
! GAATCATATGATATGGTTAA+AGG + chr2.1:64688777-64688796 None:intergenic 25.0%
! GTATAAGTAAATCTTCTTAC+AGG - chr2.1:64690167-64690186 MS.gene28291:intron 25.0%
! GTGAAAAAACAAACATAAGT+TGG + chr2.1:64689080-64689099 None:intergenic 25.0%
! TATAAGCTAATTCAGTATCA+TGG + chr2.1:64690234-64690253 None:intergenic 25.0%
! TCAAGCATTATATGAAAGAA+TGG + chr2.1:64690441-64690460 None:intergenic 25.0%
! TCACAAAAAAGAGAAACTTA+TGG - chr2.1:64689096-64689115 MS.gene28291:intron 25.0%
! TCTCAGAAATTGCTTATATA+GGG - chr2.1:64687833-64687852 MS.gene28291:intron 25.0%
! TGGTAAAACAGAGAATATTA+TGG - chr2.1:64688826-64688845 MS.gene28291:intron 25.0%
! TGTGGAAAAGATTATATGTT+TGG + chr2.1:64688308-64688327 None:intergenic 25.0%
! TTATACCTTAATGTAAGAGA+GGG - chr2.1:64690249-64690268 MS.gene28291:intron 25.0%
! TTTACTTATACTATGAGTCA+TGG + chr2.1:64690158-64690177 None:intergenic 25.0%
!! AAAAACAAACATAAGTTGGA+TGG + chr2.1:64689076-64689095 None:intergenic 25.0%
!! ACATGTTTTCTTAATTGTAC+AGG - chr2.1:64690493-64690512 MS.gene28291:intron 25.0%
!! TACAAAGTCTTATAATGTGA+AGG - chr2.1:64689946-64689965 MS.gene28291:intron 25.0%
!!! AATGTACCGTTTTTGAATTT+CGG - chr2.1:64687985-64688004 MS.gene28291:intron 25.0%
!!! GTTGATTTGATTAAGTTGAA+AGG - chr2.1:64689557-64689576 MS.gene28291:intron 25.0%
!!! TTCTTTTGATTCAGATACTA+GGG - chr2.1:64689207-64689226 MS.gene28291:intron 25.0%
!!! TTTCTTTTGATTCAGATACT+AGG - chr2.1:64689206-64689225 MS.gene28291:intron 25.0%
AAAAACCCTCTCTTACATTA+AGG + chr2.1:64690257-64690276 None:intergenic 30.0%
AAACAATACAAGGGGTTTAA+GGG + chr2.1:64689522-64689541 None:intergenic 30.0%
AAGAAATCAATGCAAAAGTG+AGG + chr2.1:64689721-64689740 None:intergenic 30.0%
ACAAGCAAACAATAAACGAA+TGG + chr2.1:64689352-64689371 None:intergenic 30.0%
AGAGGTTGTAGATAATGTAA+AGG - chr2.1:64690358-64690377 MS.gene28291:intron 30.0%
AGTTTCTGATACATATTCAC+AGG + chr2.1:64688957-64688976 None:intergenic 30.0%
ATGTTTATAGCAAAGTACGA+GGG + chr2.1:64688563-64688582 None:intergenic 30.0%
ATTGCTGATGAAATACAAAC+TGG - chr2.1:64688428-64688447 MS.gene28291:intron 30.0%
CAGATATATTAGAGATATGC+TGG + chr2.1:64687947-64687966 None:intergenic 30.0%
CTTAATCAAATCAACCAGAA+TGG + chr2.1:64689552-64689571 None:intergenic 30.0%
CTTATACCTTAATGTAAGAG+AGG - chr2.1:64690248-64690267 MS.gene28291:intron 30.0%
GAACTAAGAAACAATACAAG+GGG + chr2.1:64689530-64689549 None:intergenic 30.0%
GAATGTTTGTCTATCTTTCT+TGG - chr2.1:64690013-64690032 MS.gene28291:intron 30.0%
GGAACTAAGAAACAATACAA+GGG + chr2.1:64689531-64689550 None:intergenic 30.0%
GTAACACCGAAATTCAAAAA+CGG + chr2.1:64687994-64688013 None:intergenic 30.0%
GTCTTGATTTAACCTTGTAA+TGG - chr2.1:64689644-64689663 MS.gene28291:intron 30.0%
GTTAAATCAAGACATGCAAA+TGG + chr2.1:64689637-64689656 None:intergenic 30.0%
TACATATTCACAGGAATAGA+AGG + chr2.1:64688948-64688967 None:intergenic 30.0%
TGATATGGTTAAAGGAAGTA+AGG + chr2.1:64688769-64688788 None:intergenic 30.0%
TGGAACTAAGAAACAATACA+AGG + chr2.1:64689532-64689551 None:intergenic 30.0%
TTGCGAAGAATCATATGATA+TGG + chr2.1:64688784-64688803 None:intergenic 30.0%
! AAAGTCTTATAATGTGAAGG+AGG - chr2.1:64689949-64689968 MS.gene28291:intron 30.0%
! ATTGTTTCTTAGTTCCATTC+TGG - chr2.1:64689535-64689554 MS.gene28291:intron 30.0%
! CAATGCTTTGTTATCAAGTT+AGG - chr2.1:64687856-64687875 MS.gene28291:intron 30.0%
! CTTGTACTTTTGTGTTTTAC+TGG - chr2.1:64688806-64688825 MS.gene28291:intron 30.0%
! TATTCTGTATCCTTTTTCTG+AGG - chr2.1:64689148-64689167 MS.gene28291:intron 30.0%
!! AAAGGAAGTAAGGTATCTTT+TGG + chr2.1:64688759-64688778 None:intergenic 30.0%
!!! GTTACTTTTCACAGTACTTT+TGG - chr2.1:64689838-64689857 MS.gene28291:CDS 30.0%
!!! GTTTTCGAGTCTTCTAATAT+TGG - chr2.1:64690105-64690124 MS.gene28291:intron 30.0%
!!! TACAAGTCTTGTGACTTTTT+TGG - chr2.1:64687879-64687898 MS.gene28291:intron 30.0%
ACAGCTTTCAAATAACCATC+TGG + chr2.1:64688374-64688393 None:intergenic 35.0%
ACATCATAAGACCAATCAAG+GGG + chr2.1:64689428-64689447 None:intergenic 35.0%
AGCTTTCAAATAACCATCTG+GGG + chr2.1:64688372-64688391 None:intergenic 35.0%
ATTAGATCATGAACGAAAGC+CGG + chr2.1:64688126-64688145 None:intergenic 35.0%
CAAAAACGGTACATTTCACA+TGG + chr2.1:64687980-64687999 None:intergenic 35.0%
CACATCATAAGACCAATCAA+GGG + chr2.1:64689429-64689448 None:intergenic 35.0%
CAGCTTTCAAATAACCATCT+GGG + chr2.1:64688373-64688392 None:intergenic 35.0%
CTACAGGATGAAATATGCAA+GGG + chr2.1:64688241-64688260 None:intergenic 35.0%
CTAGGTAGATGCATTTAACT+AGG - chr2.1:64689031-64689050 MS.gene28291:CDS 35.0%
GAAACAATACAAGGGGTTTA+AGG + chr2.1:64689523-64689542 None:intergenic 35.0%
GATGTTTATAGCAAAGTACG+AGG + chr2.1:64688564-64688583 None:intergenic 35.0%
GGAAACTAAACCTCAGAAAA+AGG + chr2.1:64689161-64689180 None:intergenic 35.0%
GTGATGCTTTGTATAAAACC+TGG - chr2.1:64689284-64689303 MS.gene28291:intron 35.0%
TAATGTGAGAGCTATTCTAC+AGG + chr2.1:64688257-64688276 None:intergenic 35.0%
TCACATCATAAGACCAATCA+AGG + chr2.1:64689430-64689449 None:intergenic 35.0%
TCTACAGGATGAAATATGCA+AGG + chr2.1:64688242-64688261 None:intergenic 35.0%
TTCTTTGATCACTTCAAGTG+AGG + chr2.1:64689896-64689915 None:intergenic 35.0%
! ACTTTTCACAGTACTTTTGG+TGG - chr2.1:64689841-64689860 MS.gene28291:CDS 35.0%
! AGAGAGGGTTTTTGATCATT+TGG - chr2.1:64690264-64690283 MS.gene28291:intron 35.0%
! TACTTTTGGTGGAAATCCAT+TGG - chr2.1:64689852-64689871 MS.gene28291:CDS 35.0%
AACAAAACTCCACCTTCTGT+TGG - chr2.1:64689457-64689476 MS.gene28291:intron 40.0%
AAGACCAATCAAGGGGTTTA+AGG + chr2.1:64689421-64689440 None:intergenic 40.0%
AGACCAATCAAGGGGTTTAA+GGG + chr2.1:64689420-64689439 None:intergenic 40.0%
AGACTTCTGATTCCAACAGA+AGG + chr2.1:64689472-64689491 None:intergenic 40.0%
AGCAAGAACAGGAAAAATGC+TGG - chr2.1:64688454-64688473 MS.gene28291:intron 40.0%
AGCTTGAAACTGACACTACA+TGG - chr2.1:64688703-64688722 MS.gene28291:intron 40.0%
AGGTCATGTCGTGTATGTAA+AGG - chr2.1:64690378-64690397 MS.gene28291:intron 40.0%
ATGCCATTGTGTAGGCAATT+TGG - chr2.1:64690288-64690307 MS.gene28291:intron 40.0%
CAGATACTAGGGAAAGCTTT+GGG - chr2.1:64689218-64689237 MS.gene28291:intron 40.0%
CATTGTGTAGGCAATTTGGT+AGG - chr2.1:64690292-64690311 MS.gene28291:intron 40.0%
CTACCAAATTGCCTACACAA+TGG + chr2.1:64690294-64690313 None:intergenic 40.0%
CTTATAATGTGAAGGAGGTG+CGG - chr2.1:64689954-64689973 MS.gene28291:intron 40.0%
CTTGTCTGAGTCTTAGTCAA+TGG - chr2.1:64688975-64688994 MS.gene28291:CDS 40.0%
CTTTCAAATAACCATCTGGG+GGG + chr2.1:64688370-64688389 None:intergenic 40.0%
GAAAAAGGGAGATAGCAACA+AGG + chr2.1:64689123-64689142 None:intergenic 40.0%
GACACTACATGGCAAGAAAT+AGG - chr2.1:64688714-64688733 MS.gene28291:intron 40.0%
GCAATTTCACTAGTTCCCAT+AGG + chr2.1:64689695-64689714 None:intergenic 40.0%
GCTTTCAAATAACCATCTGG+GGG + chr2.1:64688371-64688390 None:intergenic 40.0%
GGCATGACTACTAAACATGT+TGG + chr2.1:64690418-64690437 None:intergenic 40.0%
GGTCATGTCGTGTATGTAAA+GGG - chr2.1:64690379-64690398 MS.gene28291:intron 40.0%
GTCATGTCGTGTATGTAAAG+GGG - chr2.1:64690380-64690399 MS.gene28291:intron 40.0%
TCAGATACTAGGGAAAGCTT+TGG - chr2.1:64689217-64689236 MS.gene28291:intron 40.0%
TGCATTTAACTAGGTACCAC+TGG - chr2.1:64689040-64689059 MS.gene28291:CDS 40.0%
TGTATAAAACCTGGACAGCA+TGG - chr2.1:64689293-64689312 MS.gene28291:intron 40.0%
! CAAACATAAGTTGGATGGTC+AGG + chr2.1:64689071-64689090 None:intergenic 40.0%
! GCATTGACATAGTTCTCACA+AGG - chr2.1:64690035-64690054 MS.gene28291:intron 40.0%
! TTATAATGTGAAGGAGGTGC+GGG - chr2.1:64689955-64689974 MS.gene28291:intron 40.0%
!! AAAAATGCTGGCTTGTGAGT+GGG - chr2.1:64688466-64688485 MS.gene28291:intron 40.0%
!! CAAACTGGATTAGCAAGAAC+AGG - chr2.1:64688443-64688462 MS.gene28291:intron 40.0%
CAATACAAGGGGTTTAAGGG+AGG + chr2.1:64689519-64689538 None:intergenic 45.0%
CATTTGGCATGCCATTGTGT+AGG - chr2.1:64690280-64690299 MS.gene28291:intron 45.0%
CCATTTGCAAGTATGCAGCT+AGG - chr2.1:64689013-64689032 MS.gene28291:CDS 45.0%
CCTAGCTGCATACTTGCAAA+TGG + chr2.1:64689016-64689035 None:intergenic 45.0%
CTTCTGATTCCAACAGAAGG+TGG + chr2.1:64689469-64689488 None:intergenic 45.0%
GAAAGTGGTGACAGTTGAAG+AGG - chr2.1:64690340-64690359 MS.gene28291:intron 45.0%
GATCACTTCAAGTGAGGCAA+TGG + chr2.1:64689890-64689909 None:intergenic 45.0%
GCAATTTGGTAGGAGAGGAT+TGG - chr2.1:64690302-64690321 MS.gene28291:intron 45.0%
TAAAACCTGGACAGCATGGA+AGG - chr2.1:64689297-64689316 MS.gene28291:intron 45.0%
TCAAGACATGCAAATGGCCA+TGG + chr2.1:64689631-64689650 None:intergenic 45.0%
TGAACACCAACCACTACATC+TGG + chr2.1:64688503-64688522 None:intergenic 45.0%
TGTAGGCAATTTGGTAGGAG+AGG - chr2.1:64690297-64690316 MS.gene28291:intron 45.0%
TGTGACAGTCCATCTTTGAC+CGG - chr2.1:64688104-64688123 MS.gene28291:intron 45.0%
TTAAACCTTCCATGCTGTCC+AGG + chr2.1:64689305-64689324 None:intergenic 45.0%
TTTGTGAATCCTCGTGTCCA+TGG - chr2.1:64689611-64689630 MS.gene28291:intron 45.0%
! TATAATGTGAAGGAGGTGCG+GGG - chr2.1:64689956-64689975 MS.gene28291:intron 45.0%
!! AAGAAGAGAGACTTGCTGAG+AGG - chr2.1:64689911-64689930 MS.gene28291:intron 45.0%
!! ATACTAGGGAAAGCTTTGGG+TGG - chr2.1:64689221-64689240 MS.gene28291:intron 45.0%
!! GAAAAATGCTGGCTTGTGAG+TGG - chr2.1:64688465-64688484 MS.gene28291:intron 45.0%
AACATCATCCTGTCGTCGCA+CGG - chr2.1:64688578-64688597 MS.gene28291:intron 50.0%
CCAATCAAGGGGTTTAAGGG+CGG + chr2.1:64689417-64689436 None:intergenic 50.0%
CGAAGTTCGTCCAGATGTAG+TGG - chr2.1:64688490-64688509 MS.gene28291:intron 50.0%
GAACGAAAGCCGGTCAAAGA+TGG + chr2.1:64688116-64688135 None:intergenic 50.0%
GTTCGTCCAGATGTAGTGGT+TGG - chr2.1:64688494-64688513 MS.gene28291:intron 50.0%
TCTGCAAGAACTGCGCTAAC+TGG + chr2.1:64689257-64689276 None:intergenic 50.0%
TTTACTAACCGTGCGACGAC+AGG + chr2.1:64688589-64688608 None:intergenic 50.0%
CAGGTGAAAATCCCCCCAGA+TGG - chr2.1:64688356-64688375 MS.gene28291:CDS 55.0%
CCGCCCTTAAACCCCTTGAT+TGG - chr2.1:64689414-64689433 MS.gene28291:intron 55.0%
GGATGGTCAGGTCTATCCAG+TGG + chr2.1:64689059-64689078 None:intergenic 55.0%
TGAGGCAATGGCAACTGCAC+TGG + chr2.1:64689878-64689897 None:intergenic 55.0%
TGCAAATGGCCATGGACACG+AGG + chr2.1:64689623-64689642 None:intergenic 55.0%
! ATGGCAACTGCACTGGCCAA+TGG + chr2.1:64689871-64689890 None:intergenic 55.0%
!! CTAGGGAAAGCTTTGGGTGG+AGG - chr2.1:64689224-64689243 MS.gene28291:intron 55.0%
CGGGGAAGAGGGCTGTTCAT+TGG - chr2.1:64689974-64689993 MS.gene28291:intron 60.0%
! TGAAGGAGGTGCGGGGAAGA+GGG - chr2.1:64689963-64689982 MS.gene28291:intron 60.0%
! GTGAAGGAGGTGCGGGGAAG+AGG - chr2.1:64689962-64689981 MS.gene28291:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 64687756 64690524 64687756 ID=MS.gene28291
chr2.1 mRNA 64687756 64690524 64687756 ID=MS.gene28291.t1;Parent=MS.gene28291
chr2.1 exon 64690501 64690524 64690501 ID=MS.gene28291.t1.exon1;Parent=MS.gene28291.t1
chr2.1 CDS 64690501 64690524 64690501 ID=cds.MS.gene28291.t1;Parent=MS.gene28291.t1
chr2.1 exon 64689769 64689921 64689769 ID=MS.gene28291.t1.exon2;Parent=MS.gene28291.t1
chr2.1 CDS 64689769 64689921 64689769 ID=cds.MS.gene28291.t1;Parent=MS.gene28291.t1
chr2.1 exon 64688962 64689059 64688962 ID=MS.gene28291.t1.exon3;Parent=MS.gene28291.t1
chr2.1 CDS 64688962 64689059 64688962 ID=cds.MS.gene28291.t1;Parent=MS.gene28291.t1
chr2.1 exon 64688348 64688428 64688348 ID=MS.gene28291.t1.exon4;Parent=MS.gene28291.t1
chr2.1 CDS 64688348 64688428 64688348 ID=cds.MS.gene28291.t1;Parent=MS.gene28291.t1
chr2.1 exon 64687756 64687765 64687756 ID=MS.gene28291.t1.exon5;Parent=MS.gene28291.t1
chr2.1 CDS 64687756 64687765 64687756 ID=cds.MS.gene28291.t1;Parent=MS.gene28291.t1
Gene Sequence

>MS.gene28291

ATGCAAGAGCACATTGAGAGAATAGTGAGTGTTAAACTGTAATTCTGTTCTTATGAAATGATGGTAGTTACAACAGATCTCAGAAATTGCTTATATAGGGCAATGCTTTGTTATCAAGTTAGGTACAAGTCTTGTGACTTTTTTGGCTAGCCCTTACCAATTCTATTTTCTATTCTTATGCACTTATTCCAGCATATCTCTAATATATCTGTATTCTTTGACCATGTGAAATGTACCGTTTTTGAATTTCGGTGTTACTCACATTGTATGTTTTGATCCATTTATGAAGTATAATAGTTTGCTTGCGAGAATGTAATATTATGTATGATACGATTCTTGATGATACACTGTGACAGTCCATCTTTGACCGGCTTTCGTTCATGATCTAATTTAACGATGATATCTTAATTTATCCGTCAAACTGATTTTTTTTATTGTTTTTTCAATATTAACGTTATGAAATATGGGAATTCTATTATGATCCCTTGCATATTTCATCCTGTAGAATAGCTCTCACATTATGCTCACTTTTTGTTAAGTTTTACTTTTCCAAACATATAATCTTTTCCACATTTTTTTATATGACGTTATATTTTTTTACAGGTGAAAATCCCCCCAGATGGTTATTTGAAAGCTGTTAGAGATCTTTGCTCCAAATATAACGTGTTGATGATTGCTGATGAAATACAAACTGGATTAGCAAGAACAGGAAAAATGCTGGCTTGTGAGTGGGACGAAGTTCGTCCAGATGTAGTGGTTGGTGTTCACCTTTACCTCTTATATATTCTGTATCTCTTTATTCATCCCTCGTACTTTGCTATAAACATCATCCTGTCGTCGCACGGTTAGTAAAATATGTTGCAACTCATTGCAATTTATTTTGCATCCTTTTAATAATTTAAGTTCAGAGGTGTTTTTCATTATATCAATAATCAGTTACGTTTTTCAGCTTGAAACTGACACTACATGGCAAGAAATAGGCATGTGTTCGTAATTGATTCCAAAAGATACCTTACTTCCTTTAACCATATCATATGATTCTTCGCAATGCTTGTACTTTTGTGTTTTACTGGTAAAACAGAGAATATTATGGTTGGATTAGTTTCTCTGTAATTTTAACAATTGAAAGTTGCTTCATGACAGAAAAGCTTTAAGATACAGACTTTTAGCCATATTATATCTAGTTTTTCCTTCTATTCCTGTGAATATGTATCAGAAACTTGTCTGAGTCTTAGTCAATGGAATTTTTTTATATTGCCATTTGCAAGTATGCAGCTAGGTAGATGCATTTAACTAGGTACCACTGGATAGACCTGACCATCCAACTTATGTTTGTTTTTTCACAAAAAAGAGAAACTTATGGTCCTTGTTGCTATCTCCCTTTTTCATTTTTATTCTGTATCCTTTTTCTGAGGTTTAGTTTCCTTTTTATACCTCTAATTTCTTTAACTTTCTTTTGATTCAGATACTAGGGAAAGCTTTGGGTGGAGGAATTATTCCAGTTAGCGCAGTTCTTGCAGACAAAGAAGTGATGCTTTGTATAAAACCTGGACAGCATGGAAGGTTTAATTTCTTATAACTTTTTTAAATACTCCATTCGTTTATTGTTTGCTTGTCAATAGTTGTCAAACATAAAAGGTTCTGCTGAGCATCTGTTTCCGCCCTTAAACCCCTTGATTGGTCTTATGATGTGAGTGAAGAAACAAAACTCCACCTTCTGTTGGAATCAGAAGTCTTACAATATTTAGTTTGTTTTGTTTCCTCCCTTAAACCCCTTGTATTGTTTCTTAGTTCCATTCTGGTTGATTTGATTAAGTTGAAAGGTTTCAAAAAAGTGTGCTCATTATGAAATAGATTTGTGAATCCTCGTGTCCATGGCCATTTGCATGTCTTGATTTAACCTTGTAATGGTTATTTATTTTAAAAAAAAATTCATCCTATGGGAACTAGTGAAATTGCATTCCTCACTTTTGCATTGATTTCTTTTTATGTAACTAGCCGATAATTTCATTCATTAAAATGAAATTAATTGTTTATTAAAGAAGGAAGTCAATACTTGTAATGCTAAACCTTTTTTTTTCTGTTACTTTTCACAGTACTTTTGGTGGAAATCCATTGGCCAGTGCAGTTGCCATTGCCTCACTTGAAGTGATCAAAGAAGAGAGACTTGCTGAGAGGTATTAAATGCATTACAAAGTCTTATAATGTGAAGGAGGTGCGGGGAAGAGGGCTGTTCATTGGAGTTGAGCTTAACAGCGAATGTTTGTCTATCTTTCTTGGCATTGACATAGTTCTCACAAGGAGATAAAAGAAATTACGATTTGAAGGTAATATCTATCTTTCTTGATTGTTTTCGAGTCTTCTAATATTGGATGTAGAAAGAAACAAAAGTCACGAGTCCATGACTCATAGTATAAGTAAATCTTCTTACAGGCGTTCATTCTTTAAAGTCATTATACTTTTTTGTCTTCATCGCCATGATACTGAATTAGCTTATACCTTAATGTAAGAGAGGGTTTTTGATCATTTGGCATGCCATTGTGTAGGCAATTTGGTAGGAGAGGATTGGTTTTATTTTTAATAAGAAAGTGGTGACAGTTGAAGAGGTTGTAGATAATGTAAAGGTCATGTCGTGTATGTAAAGGGGTGATGTTAAATGCCAACATGTTTAGTAGTCATGCCCCATTCTTTCATATAATGCTTGATTTGTAAAATGAAAATATAAGTAGAGTCTAAAACATGTTTTCTTAATTGTACAGGTTCAAGTAA
Protein sequence

>MS.gene28291.t1

MQEHIERIVKIPPDGYLKAVRDLCSKYNVLMIADEIQTGLARTGKMLACEWDEVRPDVVILGKALGGGIIPVSAVLADKEVMLCIKPGQHGSTFGGNPLASAVAIASLEVIKEERLAERFK