AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050767


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050767.t1 MTR_7g111880 96.000 250 9 1 1 249 1 250 3.30e-172 474
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050767.t1 AT4G15510 64.659 249 76 5 10 247 40 287 4.09e-105 306
MS.gene050767.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene050767.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene050767.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene050767.t1 AT4G15510 69.595 148 40 3 35 178 72 218 1.67e-62 196

Find 0 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCTTGATGAAAATATAT+CGG + 51769:12876-12895 MS.gene050767:CDS 15.0%
!!! ATATTTTCATCAAGAATATA+AGG - 51769:12874-12893 None:intergenic 15.0%
!! AAATAAAATAATTGTGACAG+AGG - 51769:12602-12621 None:intergenic 20.0%
!! TACATTTATCAGAAAAATTG+TGG + 51769:13103-13122 MS.gene050767:intron 20.0%
! GAATATGTTGATACATTTGA+TGG + 51769:12778-12797 MS.gene050767:CDS 25.0%
AAAACTTACGGTAATTTGAG+GGG + 51769:13151-13170 MS.gene050767:intron 30.0%
AGAAAATGTGTTTCAAGAAG+AGG + 51769:13420-13439 MS.gene050767:CDS 30.0%
CAAAACTTACGGTAATTTGA+GGG + 51769:13150-13169 MS.gene050767:intron 30.0%
CATTCAACTATCCATCAAAT+TGG + 51769:12806-12825 MS.gene050767:CDS 30.0%
CTTACTTGAATCCAATTTGA+TGG - 51769:12820-12839 None:intergenic 30.0%
TCAAATTGGATTCAAGTAAG+AGG + 51769:12820-12839 MS.gene050767:CDS 30.0%
! CAAATTGTACTATCAAGTTG+AGG + 51769:13074-13093 MS.gene050767:CDS 30.0%
! TCTTCTTGAAACACATTTTC+TGG - 51769:13421-13440 None:intergenic 30.0%
!! AATGACATTGCTTTTCTTCT+AGG - 51769:12691-12710 None:intergenic 30.0%
ATGGATGGAATTTACCGAAT+TGG - 51769:12565-12584 None:intergenic 35.0%
ATTCAAGACTGTTCAAGACT+TGG + 51769:12927-12946 MS.gene050767:CDS 35.0%
CTCTGTTCTTGCAAAACTTA+CGG + 51769:13139-13158 MS.gene050767:intron 35.0%
GCAAAACTTACGGTAATTTG+AGG + 51769:13149-13168 MS.gene050767:intron 35.0%
GTAATCTCAACTCATACAGT+TGG - 51769:13393-13412 None:intergenic 35.0%
GTCTTGAATTTCGAAGAAGA+AGG - 51769:12916-12935 None:intergenic 35.0%
TGGATGGAATTTACCGAATT+GGG - 51769:12564-12583 None:intergenic 35.0%
TTACTGAGTTTATGTCCACT+AGG + 51769:12992-13011 MS.gene050767:CDS 35.0%
TTCAAGACTGTTCAAGACTT+GGG + 51769:12928-12947 MS.gene050767:CDS 35.0%
! GGGTGTCTTTTATATGGTAA+TGG + 51769:13171-13190 MS.gene050767:intron 35.0%
!!! TGGTGCTGATATTTTCTTCA+GGG + 51769:12846-12865 MS.gene050767:CDS 35.0%
!!! TTTGAGGGGTGTCTTTTATA+TGG + 51769:13165-13184 MS.gene050767:intron 35.0%
ACATATTCACGAAACACACG+AGG - 51769:12766-12785 None:intergenic 40.0%
ACCCGTTGTTGTGATTGTAT+TGG - 51769:12478-12497 None:intergenic 40.0%
ACTTACATTCTCTCCGACAT+TGG + 51769:12721-12740 MS.gene050767:CDS 40.0%
AGAGGAGAAAGCTCATGAAA+TGG - 51769:12584-12603 None:intergenic 40.0%
ATGGAAGTGAAATGGTGACA+TGG - 51769:12514-12533 None:intergenic 40.0%
GAATTTACCGAATTGGGTGT+GGG - 51769:12558-12577 None:intergenic 40.0%
GAGAAAGCTCATGAAATGGA+TGG - 51769:12580-12599 None:intergenic 40.0%
GCCAATACAATCACAACAAC+GGG + 51769:12474-12493 MS.gene050767:CDS 40.0%
GGCCAATACAATCACAACAA+CGG + 51769:12473-12492 MS.gene050767:CDS 40.0%
TAGCGCTAGACTACAATGTA+TGG - 51769:13199-13218 None:intergenic 40.0%
TGCTAATGCTGTACCAATGT+CGG - 51769:12737-12756 None:intergenic 40.0%
TGCTGTTATGCCACAAGATA+GGG + 51769:13312-13331 MS.gene050767:CDS 40.0%
TGGAAGTGAAATGGTGACAT+GGG - 51769:12513-12532 None:intergenic 40.0%
TTTCAACTCCTAGAACAGAC+AGG - 51769:13366-13385 None:intergenic 40.0%
! TTGCTGTTATGCCACAAGAT+AGG + 51769:13311-13330 MS.gene050767:CDS 40.0%
!! TGTGTGTTGTTTGTGTTGCT+AGG + 51769:13255-13274 MS.gene050767:intron 40.0%
!!! CTGGTGCTGATATTTTCTTC+AGG + 51769:12845-12864 MS.gene050767:CDS 40.0%
ACGTTTAACACCAAGCCTAG+TGG - 51769:13010-13029 None:intergenic 45.0%
AGTGAAATGGTGACATGGGT+CGG - 51769:12509-12528 None:intergenic 45.0%
ATCATCAGCAACTCTCTCAG+AGG - 51769:13052-13071 None:intergenic 45.0%
CATTTCTCCCACACCCAATT+CGG + 51769:12548-12567 MS.gene050767:CDS 45.0%
GAGTGAACTTCGTCAAGTCA+TGG + 51769:13444-13463 MS.gene050767:CDS 45.0%
GAGTTTATGTCCACTAGGCT+TGG + 51769:12997-13016 MS.gene050767:CDS 45.0%
GGAATTTACCGAATTGGGTG+TGG - 51769:12559-12578 None:intergenic 45.0%
GGCTTCGTTTCGAGCAAATA+AGG + 51769:13465-13484 MS.gene050767:CDS 45.0%
TCTGAGAGAGTTGCTGATGA+TGG + 51769:13051-13070 MS.gene050767:CDS 45.0%
TGTTATGCCACAAGATAGGG+TGG + 51769:13315-13334 MS.gene050767:CDS 45.0%
! TGGATTCAAGTAAGAGGTGC+TGG + 51769:12826-12845 MS.gene050767:CDS 45.0%
!! ACTTTCTTCCCAGCTTGTTG+AGG - 51769:12958-12977 None:intergenic 45.0%
AAACGCACCACCCTATCTTG+TGG - 51769:13325-13344 None:intergenic 50.0%
ACAAGATAGGGTGGTGCGTT+TGG + 51769:13324-13343 MS.gene050767:CDS 50.0%
ATAGGGTGGTGCGTTTGGAA+TGG + 51769:13329-13348 MS.gene050767:CDS 50.0%
ATCAGCAACTCTCTCAGAGG+TGG - 51769:13049-13068 None:intergenic 50.0%
CGAATTGGGTGTGGGAGAAA+TGG - 51769:12550-12569 None:intergenic 50.0%
CGAGGGGAATGGAAGTGAAA+TGG - 51769:12522-12541 None:intergenic 50.0%
GTGAAATGGTGACATGGGTC+GGG - 51769:12508-12527 None:intergenic 50.0%
! TAGGGTGGTGCGTTTGGAAT+GGG + 51769:13330-13349 MS.gene050767:CDS 50.0%
! TGCGTTTGGAATGGGATAGG+AGG + 51769:13338-13357 MS.gene050767:CDS 50.0%
! TGGTGCGTTTGGAATGGGAT+AGG + 51769:13335-13354 MS.gene050767:CDS 50.0%
!! AGGAGGTACCTGTCTGTTCT+AGG + 51769:13355-13374 MS.gene050767:CDS 50.0%
AAATGGAGCAGCGAGGGGAA+TGG - 51769:12533-12552 None:intergenic 55.0%
TGGGAGAAATGGAGCAGCGA+GGG - 51769:12539-12558 None:intergenic 55.0%
TGGGTTCGCCTCAACAAGCT+GGG + 51769:12947-12966 MS.gene050767:CDS 55.0%
TTGGGTTCGCCTCAACAAGC+TGG + 51769:12946-12965 MS.gene050767:CDS 55.0%
GGGAGAAATGGAGCAGCGAG+GGG - 51769:12538-12557 None:intergenic 60.0%
GTGGGAGAAATGGAGCAGCG+AGG - 51769:12540-12559 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
51769 gene 12471 13498 12471 ID=MS.gene050767
51769 mRNA 12471 13498 12471 ID=MS.gene050767.t1;Parent=MS.gene050767
51769 exon 12471 12569 12471 ID=MS.gene050767.t1.exon1;Parent=MS.gene050767.t1
51769 CDS 12471 12569 12471 ID=cds.MS.gene050767.t1;Parent=MS.gene050767.t1
51769 exon 12667 13095 12667 ID=MS.gene050767.t1.exon2;Parent=MS.gene050767.t1
51769 CDS 12667 13095 12667 ID=cds.MS.gene050767.t1;Parent=MS.gene050767.t1
51769 exon 13277 13498 13277 ID=MS.gene050767.t1.exon3;Parent=MS.gene050767.t1
51769 CDS 13277 13498 13277 ID=cds.MS.gene050767.t1;Parent=MS.gene050767.t1
Gene Sequence

>MS.gene050767

ATGGCCAATACAATCACAACAACGGGTTTTCTAGCCCGACCCATGTCACCATTTCACTTCCATTCCCCTCGCTGCTCCATTTCTCCCACACCCAATTCGGTAAATTCCATCCATTTCATGAGCTTTCTCCTCTGTCACAATTATTTTATTTTATTTACTAAATCTTTGATTTTTGTCTCTGCTAATATATAATCAGATTAAAGCAGCTTTTGCAGTTCCTAGAAGAAAAGCAATGTCATTAATCTTATCAACTTACATTCTCTCCGACATTGGTACAGCATTAGCACAACAACCTCGTGTGTTTCGTGAATATGTTGATACATTTGATGGTTATTCATTCAACTATCCATCAAATTGGATTCAAGTAAGAGGTGCTGGTGCTGATATTTTCTTCAGGGATCCTTATATTCTTGATGAAAATATATCGGTCGAAGTTTCTTCGCCTTCTTCTTCGAAATTCAAGACTGTTCAAGACTTGGGTTCGCCTCAACAAGCTGGGAAGAAAGTTCTTGATCAGTATCTTACTGAGTTTATGTCCACTAGGCTTGGTGTTAAACGTGAATCTAATATTCTTTCCACCTCTGAGAGAGTTGCTGATGATGGCAAATTGTACTATCAAGTTGAGGTTCGTTTACATTTATCAGAAAAATTGTGGTTAATCACGTTAACTCTGTTCTTGCAAAACTTACGGTAATTTGAGGGGTGTCTTTTATATGGTAATGGAACCATACATTGTAGTCTAGCGCTACTTGTTGCAAAATGACATTAAATGATTGATTGATTGTGTGTGTTGTTTGTGTTGCTAGGCGAACATAAAGTCATATGCTAGTAACAACGAGCTTGCTGTTATGCCACAAGATAGGGTGGTGCGTTTGGAATGGGATAGGAGGTACCTGTCTGTTCTAGGAGTTGAAAACAACCAACTGTATGAGTTGAGATTACAAGTGCCAGAAAATGTGTTTCAAGAAGAGGAGAGTGAACTTCGTCAAGTCATGGCTTCGTTTCGAGCAAATAAGGTTGTTCGTTAG

Protein sequence

>MS.gene050767.t1

MANTITTTGFLARPMSPFHFHSPRCSISPTPNSIKAAFAVPRRKAMSLILSTYILSDIGTALAQQPRVFREYVDTFDGYSFNYPSNWIQVRGAGADIFFRDPYILDENISVEVSSPSSSKFKTVQDLGSPQQAGKKVLDQYLTEFMSTRLGVKRESNILSTSERVADDGKLYYQVEANIKSYASNNELAVMPQDRVVRLEWDRRYLSVLGVENNQLYELRLQVPENVFQEEESELRQVMASFRANKVVR