AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050770.t1 MTR_7g111880 96.000 250 9 1 1 249 1 250 3.30e-172 474
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050770.t1 AT4G15510 64.659 249 76 5 10 247 40 287 4.09e-105 306
MS.gene050770.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene050770.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene050770.t1 AT4G15510 73.039 204 52 2 46 247 3 205 2.02e-103 298
MS.gene050770.t1 AT4G15510 69.595 148 40 3 35 178 72 218 1.67e-62 196

Find 0 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTCTTGATGAAAATATAT+CGG + 51772:4013-4032 MS.gene050770:CDS 15.0%
!!! ATATTTTCATCAAGAATATA+AGG - 51772:4011-4030 None:intergenic 15.0%
!! AAATAAAATAATTGTGACAG+AGG - 51772:3739-3758 None:intergenic 20.0%
!! TACATTTATCAGAAAAATTG+TGG + 51772:4240-4259 MS.gene050770:intron 20.0%
! GAATATGTTGATACATTTGA+TGG + 51772:3915-3934 MS.gene050770:CDS 25.0%
AAAACTTACGGTAATTTGAG+GGG + 51772:4288-4307 MS.gene050770:intron 30.0%
AGAAAATGTGTTTCAAGAAG+AGG + 51772:4557-4576 MS.gene050770:CDS 30.0%
CAAAACTTACGGTAATTTGA+GGG + 51772:4287-4306 MS.gene050770:intron 30.0%
CATTCAACTATCCATCAAAT+TGG + 51772:3943-3962 MS.gene050770:CDS 30.0%
CTTACTTGAATCCAATTTGA+TGG - 51772:3957-3976 None:intergenic 30.0%
TCAAATTGGATTCAAGTAAG+AGG + 51772:3957-3976 MS.gene050770:CDS 30.0%
! CAAATTGTACTATCAAGTTG+AGG + 51772:4211-4230 MS.gene050770:CDS 30.0%
! TCTTCTTGAAACACATTTTC+TGG - 51772:4558-4577 None:intergenic 30.0%
!! AATGACATTGCTTTTCTTCT+AGG - 51772:3828-3847 None:intergenic 30.0%
ATGGATGGAATTTACCGAAT+TGG - 51772:3702-3721 None:intergenic 35.0%
ATTCAAGACTGTTCAAGACT+TGG + 51772:4064-4083 MS.gene050770:CDS 35.0%
CTCTGTTCTTGCAAAACTTA+CGG + 51772:4276-4295 MS.gene050770:intron 35.0%
GCAAAACTTACGGTAATTTG+AGG + 51772:4286-4305 MS.gene050770:intron 35.0%
GTAATCTCAACTCATACAGT+TGG - 51772:4530-4549 None:intergenic 35.0%
GTCTTGAATTTCGAAGAAGA+AGG - 51772:4053-4072 None:intergenic 35.0%
TGGATGGAATTTACCGAATT+GGG - 51772:3701-3720 None:intergenic 35.0%
TTACTGAGTTTATGTCCACT+AGG + 51772:4129-4148 MS.gene050770:CDS 35.0%
TTCAAGACTGTTCAAGACTT+GGG + 51772:4065-4084 MS.gene050770:CDS 35.0%
! GGGTGTCTTTTATATGGTAA+TGG + 51772:4308-4327 MS.gene050770:intron 35.0%
!!! TGGTGCTGATATTTTCTTCA+GGG + 51772:3983-4002 MS.gene050770:CDS 35.0%
!!! TTTGAGGGGTGTCTTTTATA+TGG + 51772:4302-4321 MS.gene050770:intron 35.0%
ACATATTCACGAAACACACG+AGG - 51772:3903-3922 None:intergenic 40.0%
ACCCGTTGTTGTGATTGTAT+TGG - 51772:3615-3634 None:intergenic 40.0%
ACTTACATTCTCTCCGACAT+TGG + 51772:3858-3877 MS.gene050770:CDS 40.0%
AGAGGAGAAAGCTCATGAAA+TGG - 51772:3721-3740 None:intergenic 40.0%
ATGGAAGTGAAATGGTGACA+TGG - 51772:3651-3670 None:intergenic 40.0%
GAATTTACCGAATTGGGTGT+GGG - 51772:3695-3714 None:intergenic 40.0%
GAGAAAGCTCATGAAATGGA+TGG - 51772:3717-3736 None:intergenic 40.0%
GCCAATACAATCACAACAAC+GGG + 51772:3611-3630 MS.gene050770:CDS 40.0%
GGCCAATACAATCACAACAA+CGG + 51772:3610-3629 MS.gene050770:CDS 40.0%
TAGCGCTAGACTACAATGTA+TGG - 51772:4336-4355 None:intergenic 40.0%
TGCTAATGCTGTACCAATGT+CGG - 51772:3874-3893 None:intergenic 40.0%
TGCTGTTATGCCACAAGATA+GGG + 51772:4449-4468 MS.gene050770:CDS 40.0%
TGGAAGTGAAATGGTGACAT+GGG - 51772:3650-3669 None:intergenic 40.0%
TTTCAACTCCTAGAACAGAC+AGG - 51772:4503-4522 None:intergenic 40.0%
! TTGCTGTTATGCCACAAGAT+AGG + 51772:4448-4467 MS.gene050770:CDS 40.0%
!! TGTGTGTTGTTTGTGTTGCT+AGG + 51772:4392-4411 MS.gene050770:intron 40.0%
!!! CTGGTGCTGATATTTTCTTC+AGG + 51772:3982-4001 MS.gene050770:CDS 40.0%
ACGTTTAACACCAAGCCTAG+TGG - 51772:4147-4166 None:intergenic 45.0%
AGTGAAATGGTGACATGGGT+CGG - 51772:3646-3665 None:intergenic 45.0%
ATCATCAGCAACTCTCTCAG+AGG - 51772:4189-4208 None:intergenic 45.0%
CATTTCTCCCACACCCAATT+CGG + 51772:3685-3704 MS.gene050770:CDS 45.0%
GAGTGAACTTCGTCAAGTCA+TGG + 51772:4581-4600 MS.gene050770:CDS 45.0%
GAGTTTATGTCCACTAGGCT+TGG + 51772:4134-4153 MS.gene050770:CDS 45.0%
GGAATTTACCGAATTGGGTG+TGG - 51772:3696-3715 None:intergenic 45.0%
GGCTTCGTTTCGAGCAAATA+AGG + 51772:4602-4621 MS.gene050770:CDS 45.0%
TCTGAGAGAGTTGCTGATGA+TGG + 51772:4188-4207 MS.gene050770:CDS 45.0%
TGTTATGCCACAAGATAGGG+TGG + 51772:4452-4471 MS.gene050770:CDS 45.0%
! TGGATTCAAGTAAGAGGTGC+TGG + 51772:3963-3982 MS.gene050770:CDS 45.0%
!! ACTTTCTTCCCAGCTTGTTG+AGG - 51772:4095-4114 None:intergenic 45.0%
AAACGCACCACCCTATCTTG+TGG - 51772:4462-4481 None:intergenic 50.0%
ACAAGATAGGGTGGTGCGTT+TGG + 51772:4461-4480 MS.gene050770:CDS 50.0%
ATAGGGTGGTGCGTTTGGAA+TGG + 51772:4466-4485 MS.gene050770:CDS 50.0%
ATCAGCAACTCTCTCAGAGG+TGG - 51772:4186-4205 None:intergenic 50.0%
CGAATTGGGTGTGGGAGAAA+TGG - 51772:3687-3706 None:intergenic 50.0%
CGAGGGGAATGGAAGTGAAA+TGG - 51772:3659-3678 None:intergenic 50.0%
GTGAAATGGTGACATGGGTC+GGG - 51772:3645-3664 None:intergenic 50.0%
! TAGGGTGGTGCGTTTGGAAT+GGG + 51772:4467-4486 MS.gene050770:CDS 50.0%
! TGCGTTTGGAATGGGATAGG+AGG + 51772:4475-4494 MS.gene050770:CDS 50.0%
! TGGTGCGTTTGGAATGGGAT+AGG + 51772:4472-4491 MS.gene050770:CDS 50.0%
!! AGGAGGTACCTGTCTGTTCT+AGG + 51772:4492-4511 MS.gene050770:CDS 50.0%
AAATGGAGCAGCGAGGGGAA+TGG - 51772:3670-3689 None:intergenic 55.0%
TGGGAGAAATGGAGCAGCGA+GGG - 51772:3676-3695 None:intergenic 55.0%
TGGGTTCGCCTCAACAAGCT+GGG + 51772:4084-4103 MS.gene050770:CDS 55.0%
TTGGGTTCGCCTCAACAAGC+TGG + 51772:4083-4102 MS.gene050770:CDS 55.0%
GGGAGAAATGGAGCAGCGAG+GGG - 51772:3675-3694 None:intergenic 60.0%
GTGGGAGAAATGGAGCAGCG+AGG - 51772:3677-3696 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
51772 gene 3608 4635 3608 ID=MS.gene050770
51772 mRNA 3608 4635 3608 ID=MS.gene050770.t1;Parent=MS.gene050770
51772 exon 3608 3706 3608 ID=MS.gene050770.t1.exon1;Parent=MS.gene050770.t1
51772 CDS 3608 3706 3608 ID=cds.MS.gene050770.t1;Parent=MS.gene050770.t1
51772 exon 3804 4232 3804 ID=MS.gene050770.t1.exon2;Parent=MS.gene050770.t1
51772 CDS 3804 4232 3804 ID=cds.MS.gene050770.t1;Parent=MS.gene050770.t1
51772 exon 4414 4635 4414 ID=MS.gene050770.t1.exon3;Parent=MS.gene050770.t1
51772 CDS 4414 4635 4414 ID=cds.MS.gene050770.t1;Parent=MS.gene050770.t1
Gene Sequence

>MS.gene050770

ATGGCCAATACAATCACAACAACGGGTTTTCTAGCCCGACCCATGTCACCATTTCACTTCCATTCCCCTCGCTGCTCCATTTCTCCCACACCCAATTCGGTAAATTCCATCCATTTCATGAGCTTTCTCCTCTGTCACAATTATTTTATTTTATTTACTAAATCTTTGATTTTTGTCTCTGCTAATATATAATCAGATTAAAGCAGCTTTTGCAGTTCCTAGAAGAAAAGCAATGTCATTAATCTTATCAACTTACATTCTCTCCGACATTGGTACAGCATTAGCACAACAACCTCGTGTGTTTCGTGAATATGTTGATACATTTGATGGTTATTCATTCAACTATCCATCAAATTGGATTCAAGTAAGAGGTGCTGGTGCTGATATTTTCTTCAGGGATCCTTATATTCTTGATGAAAATATATCGGTCGAAGTTTCTTCGCCTTCTTCTTCGAAATTCAAGACTGTTCAAGACTTGGGTTCGCCTCAACAAGCTGGGAAGAAAGTTCTTGATCAGTATCTTACTGAGTTTATGTCCACTAGGCTTGGTGTTAAACGTGAATCTAATATTCTTTCCACCTCTGAGAGAGTTGCTGATGATGGCAAATTGTACTATCAAGTTGAGGTTCGTTTACATTTATCAGAAAAATTGTGGTTAATCACGTTAACTCTGTTCTTGCAAAACTTACGGTAATTTGAGGGGTGTCTTTTATATGGTAATGGAACCATACATTGTAGTCTAGCGCTACTTGTTGCAAAATGACATTAAATGATTGATTGATTGTGTGTGTTGTTTGTGTTGCTAGGCGAACATAAAGTCATATGCTAGTAACAACGAGCTTGCTGTTATGCCACAAGATAGGGTGGTGCGTTTGGAATGGGATAGGAGGTACCTGTCTGTTCTAGGAGTTGAAAACAACCAACTGTATGAGTTGAGATTACAAGTGCCAGAAAATGTGTTTCAAGAAGAGGAGAGTGAACTTCGTCAAGTCATGGCTTCGTTTCGAGCAAATAAGGTTGTTCGTTAG

Protein sequence

>MS.gene050770.t1

MANTITTTGFLARPMSPFHFHSPRCSISPTPNSIKAAFAVPRRKAMSLILSTYILSDIGTALAQQPRVFREYVDTFDGYSFNYPSNWIQVRGAGADIFFRDPYILDENISVEVSSPSSSKFKTVQDLGSPQQAGKKVLDQYLTEFMSTRLGVKRESNILSTSERVADDGKLYYQVEANIKSYASNNELAVMPQDRVVRLEWDRRYLSVLGVENNQLYELRLQVPENVFQEEESELRQVMASFRANKVVR