AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29888


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29888.t1 MTR_3g047140 76.344 279 60 3 1 274 1 278 1.04e-145 410
MS.gene29888.t1 MTR_0163s0020 64.621 277 92 2 1 273 2 276 1.75e-110 320
MS.gene29888.t1 MTR_0684s0020 64.621 277 92 2 1 273 1 275 2.87e-110 320
MS.gene29888.t1 MTR_3g054170 54.000 250 110 3 9 257 9 254 3.30e-82 248
MS.gene29888.t1 MTR_3g054180 53.571 252 110 4 9 257 13 260 8.87e-80 242
MS.gene29888.t1 MTR_1g090973 50.190 263 122 5 1 257 1 260 1.30e-77 236
MS.gene29888.t1 MTR_3g054200 50.800 250 118 3 9 257 9 254 2.88e-74 228
MS.gene29888.t1 MTR_1g090943 48.679 265 127 7 10 271 15 273 5.53e-69 215
MS.gene29888.t1 MTR_1g090960 45.357 280 103 8 1 269 1 241 5.37e-63 198
MS.gene29888.t1 MTR_3g027280 48.239 284 123 14 1 267 1 277 1.15e-60 194
MS.gene29888.t1 MTR_1g090937 42.908 282 134 13 1 267 1 270 1.28e-50 167
MS.gene29888.t1 MTR_1g090957 45.000 280 120 15 1 264 1 262 8.88e-50 165
MS.gene29888.t1 MTR_1g090930 41.319 288 138 11 1 269 1 276 4.49e-48 161
MS.gene29888.t1 MTR_4g023810 41.736 242 129 7 26 263 27 260 1.55e-43 149
MS.gene29888.t1 MTR_8g068040 38.996 259 136 10 10 255 10 259 4.62e-42 145
MS.gene29888.t1 MTR_8g023510 40.755 265 143 10 1 257 1 259 8.98e-42 145
MS.gene29888.t1 MTR_8g068030 37.729 273 142 11 1 255 1 263 6.44e-41 142
MS.gene29888.t1 MTR_8g068050 37.736 265 140 8 10 259 21 275 5.74e-38 141
MS.gene29888.t1 MTR_4g093080 34.601 263 154 8 8 258 4 260 3.54e-34 131
MS.gene29888.t1 MTR_5g031160 37.200 250 135 8 26 257 42 287 5.94e-34 124
MS.gene29888.t1 MTR_5g031090 34.413 247 140 8 30 257 32 275 1.96e-33 123
MS.gene29888.t1 MTR_8g067940 35.849 265 145 10 11 255 20 279 7.55e-33 122
MS.gene29888.t1 MTR_8g067980 37.083 240 128 11 32 255 25 257 8.92e-33 121
MS.gene29888.t1 MTR_5g031120 36.992 246 132 8 31 257 38 279 1.11e-32 121
MS.gene29888.t1 MTR_5g031030 34.568 243 141 8 30 257 35 274 2.49e-32 120
MS.gene29888.t1 MTR_5g031140 38.115 244 131 9 31 257 46 286 5.03e-32 119
MS.gene29888.t1 MTR_8g067990 37.759 241 125 12 32 255 25 257 5.76e-32 119
MS.gene29888.t1 MTR_4g056320 35.971 278 127 12 2 270 6 241 3.81e-31 117
MS.gene29888.t1 MTR_5g031100 35.772 246 134 9 31 257 38 278 5.67e-30 114
MS.gene29888.t1 MTR_7g115740 34.335 233 124 11 46 258 38 261 1.01e-29 118
MS.gene29888.t1 MTR_8g067735 32.103 271 165 9 9 267 25 288 1.03e-29 118
MS.gene29888.t1 MTR_4g093110 34.799 273 133 13 10 257 12 264 2.41e-28 114
MS.gene29888.t1 MTR_7g116130 32.721 272 148 13 11 260 6 264 1.65e-27 111
MS.gene29888.t1 MTR_4g093140 33.456 272 138 11 10 257 12 264 2.73e-27 111
MS.gene29888.t1 MTR_4g093040 32.794 247 144 9 11 240 13 254 3.07e-27 110
MS.gene29888.t1 MTR_8g067960 37.168 226 120 10 46 257 24 241 4.26e-26 103
MS.gene29888.t1 MTR_4g093040 39.306 173 88 7 82 240 3 172 8.93e-26 106
MS.gene29888.t1 MTR_1g014240 35.407 209 118 6 71 265 78 283 9.53e-25 103
MS.gene29888.t1 MTR_8g067630 33.040 227 131 8 31 243 30 249 1.34e-23 100
MS.gene29888.t1 MTR_8g067930 29.889 271 171 8 9 267 6 269 1.66e-23 100
MS.gene29888.t1 MTR_4g093070 34.328 268 143 9 10 257 12 266 2.21e-23 99.8
MS.gene29888.t1 MTR_8g067720 30.153 262 160 9 11 260 10 260 3.71e-23 99.0
MS.gene29888.t1 MTR_3g463370 31.734 271 129 9 10 269 9 234 1.03e-22 94.0
MS.gene29888.t1 MTR_8g067690 29.412 238 154 6 31 257 60 294 9.00e-21 92.0
MS.gene29888.t1 MTR_5g025020 33.690 187 103 7 72 240 88 271 7.27e-19 86.7
MS.gene29888.t1 MTR_4g093050 30.980 255 155 10 16 253 12 262 7.36e-19 86.7
MS.gene29888.t1 MTR_7g063010 30.544 239 127 11 42 254 32 257 9.42e-16 77.4
MS.gene29888.t1 MTR_1g048360 28.458 253 162 6 15 256 17 261 1.47e-15 76.6
MS.gene29888.t1 MTR_5g077100 32.273 220 129 7 50 259 45 254 2.58e-15 75.9
MS.gene29888.t1 MTR_7g062940 28.839 267 157 12 9 254 2 256 6.36e-14 72.0
MS.gene29888.t1 MTR_2g090410 34.694 147 85 6 46 186 34 175 6.54e-14 72.0
MS.gene29888.t1 MTR_7g115740 32.593 135 70 7 46 167 38 164 1.38e-13 70.9
MS.gene29888.t1 MTR_2g090250 26.872 227 148 5 44 253 34 259 5.44e-13 68.9
MS.gene29888.t1 MTR_3g068025 27.957 279 151 12 9 254 7 268 1.52e-12 67.8
MS.gene29888.t1 MTR_5g068770 27.570 214 132 7 72 267 81 289 1.53e-12 67.8
MS.gene29888.t1 MTR_7g062890 30.244 205 116 9 72 254 58 257 4.51e-11 63.2
MS.gene29888.t1 MTR_8g068050 28.916 166 104 4 10 170 21 177 9.42e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29888.t1 AT5G10530 35.019 257 139 13 19 258 10 255 5.93e-24 101
MS.gene29888.t1 AT5G65600 34.211 190 106 7 71 243 76 263 7.99e-20 89.4
MS.gene29888.t1 AT5G55830 31.716 268 153 11 10 255 7 266 2.39e-17 82.0
MS.gene29888.t1 AT5G03140 29.245 212 135 6 50 251 51 257 2.74e-15 75.9
MS.gene29888.t1 AT5G06740 28.571 259 155 13 18 256 11 259 7.66e-14 71.6
MS.gene29888.t1 AT5G06740 28.571 259 155 13 18 256 23 271 7.78e-14 71.6
MS.gene29888.t1 AT4G29050 27.363 201 127 5 72 254 60 259 2.94e-13 70.1
MS.gene29888.t1 AT4G29050 27.363 201 127 5 72 254 74 273 3.38e-13 69.7
MS.gene29888.t1 AT3G55550 29.583 240 142 9 34 253 26 258 6.17e-13 68.9
MS.gene29888.t1 AT1G53080 29.258 229 129 10 59 258 57 281 5.13e-11 62.4
MS.gene29888.t1 AT4G04960 33.775 151 90 4 46 190 35 181 8.62e-11 62.4

Find 51 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACCAATGCTAACTGTTTC+TGG 0.178047 3.3:-37874853 None:intergenic
AGATTTGGAATTACACTATT+TGG 0.215421 3.3:-37874532 None:intergenic
ATTAACCCTTATACGATTAT+AGG 0.243083 3.3:+37874367 MS.gene29888:CDS
CCATCTGTGTTGTTAAGATT+TGG 0.245140 3.3:-37874547 None:intergenic
GCACGACCTATAATCGTATA+AGG 0.280994 3.3:-37874373 None:intergenic
GATCCTAACAATGCACATAT+TGG 0.297717 3.3:+37874652 MS.gene29888:CDS
TTGATAATTATCACAATGAT+TGG 0.305021 3.3:+37874629 MS.gene29888:CDS
TCAAGGGAATGCTGTCATTC+AGG 0.316108 3.3:+37874318 MS.gene29888:CDS
ACAGTTAGCATTGGTCTATC+TGG 0.319313 3.3:+37874859 MS.gene29888:CDS
ATTCAACACCCGTTCCCATT+TGG 0.342088 3.3:+37874401 MS.gene29888:CDS
CAACACAGATGGTGGAGATC+TGG 0.350959 3.3:+37874558 MS.gene29888:CDS
GGGAACGGGTGTTGAATATA+AGG 0.357167 3.3:-37874395 None:intergenic
TTCAACACCCGTTCCCATTT+GGG 0.360630 3.3:+37874402 MS.gene29888:CDS
GAAACTCACTTGGAGCTTAC+TGG 0.370616 3.3:+37874340 MS.gene29888:CDS
CCCCATGGGATTCACAAAAC+GGG 0.386898 3.3:+37874710 MS.gene29888:CDS
ACTGCTTTCAATCGATTTGT+TGG 0.405940 3.3:+37874598 MS.gene29888:CDS
TGAAGATGACTAACAGAAAC+TGG 0.410445 3.3:-37874195 None:intergenic
ATCCTAACAATGCACATATT+GGG 0.411998 3.3:+37874653 MS.gene29888:CDS
CCCATTTGGGATAGTGCAAC+TGG 0.412436 3.3:+37874415 MS.gene29888:CDS
GCCAGTTGCACTATCCCAAA+TGG 0.414802 3.3:-37874416 None:intergenic
GACTTAGCTGATTACATCTC+TGG 0.418401 3.3:+37874277 MS.gene29888:CDS
ACTAACAGAAACTGGTTAGT+TGG 0.419127 3.3:-37874187 None:intergenic
GAATGACAGCATTCCCTTGA+AGG 0.432002 3.3:-37874315 None:intergenic
TCCCCATGGGATTCACAAAA+CGG 0.443828 3.3:+37874709 MS.gene29888:CDS
TGATAATTATCACAATGATT+GGG 0.445401 3.3:+37874630 MS.gene29888:CDS
ATCCCAATATGTGCATTGTT+AGG 0.450564 3.3:-37874655 None:intergenic
GACCAATGCTAACTGTTTCT+GGG 0.454186 3.3:-37874852 None:intergenic
TGAATCCCATGGGGATGTCT+TGG 0.462545 3.3:-37874701 None:intergenic
GTTTCTCTCATTGACGAGAA+TGG 0.472278 3.3:+37874784 MS.gene29888:CDS
CTCAGTTACTAGCAAGAGAA+AGG 0.473501 3.3:-37874224 None:intergenic
GCACTATCCCAAATGGGAAC+GGG 0.488710 3.3:-37874409 None:intergenic
AACACAGATGGTGGAGATCT+GGG 0.502642 3.3:+37874559 MS.gene29888:CDS
CACGACCTATAATCGTATAA+GGG 0.505615 3.3:-37874372 None:intergenic
TTTGGAATTACACTATTTGG+TGG 0.521911 3.3:-37874529 None:intergenic
CCAGTTGCACTATCCCAAAT+GGG 0.537384 3.3:-37874415 None:intergenic
TGCACTATCCCAAATGGGAA+CGG 0.540036 3.3:-37874410 None:intergenic
AATGACAGCATTCCCTTGAA+GGG 0.551329 3.3:-37874314 None:intergenic
GGTAGTTATCCACGAGCTGA+TGG 0.555952 3.3:+37874487 MS.gene29888:CDS
CTCCCAGAAACAGTTAGCAT+TGG 0.565328 3.3:+37874850 MS.gene29888:CDS
CCAAATCTTAACAACACAGA+TGG 0.577172 3.3:+37874547 MS.gene29888:CDS
AATCTTAACAACACAGATGG+TGG 0.587455 3.3:+37874550 MS.gene29888:CDS
TGTCATTCAGGAAACTCACT+TGG 0.593945 3.3:+37874330 MS.gene29888:CDS
CAAATCAACGACTTGAGCAA+CGG 0.597604 3.3:-37874818 None:intergenic
TTCATCCAAGACATCCCCAT+GGG 0.607724 3.3:+37874696 MS.gene29888:CDS
AAGATAAGACCATCAGCTCG+TGG 0.612801 3.3:-37874496 None:intergenic
GATGTAATCAGCTAAGTCGA+AGG 0.627754 3.3:-37874272 None:intergenic
AAATATATGTCATTGAAACA+TGG 0.630792 3.3:+37874905 MS.gene29888:CDS
CCCATGGGATTCACAAAACG+GGG 0.631871 3.3:+37874711 MS.gene29888:CDS
AGTATGATTTATGTTCTCTG+AGG 0.647177 3.3:-37874968 None:intergenic
TTTCATCCAAGACATCCCCA+TGG 0.656398 3.3:+37874695 MS.gene29888:CDS
GCTCGTGGATAACTACCGAA+CGG 0.706851 3.3:-37874481 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATGTCATTGAAACA+TGG + chr3.3:37874905-37874924 MS.gene29888:CDS 20.0%
!! TGATAATTATCACAATGATT+GGG + chr3.3:37874630-37874649 MS.gene29888:CDS 20.0%
!!! TTGATAATTATCACAATGAT+TGG + chr3.3:37874629-37874648 MS.gene29888:CDS 20.0%
! AAAAAACTTTAACCCTTCAA+GGG + chr3.3:37874302-37874321 MS.gene29888:CDS 25.0%
! ATTAACCCTTATACGATTAT+AGG + chr3.3:37874367-37874386 MS.gene29888:CDS 25.0%
!! AGATTTGGAATTACACTATT+TGG - chr3.3:37874535-37874554 None:intergenic 25.0%
AGTATGATTTATGTTCTCTG+AGG - chr3.3:37874971-37874990 None:intergenic 30.0%
ATCCTAACAATGCACATATT+GGG + chr3.3:37874653-37874672 MS.gene29888:CDS 30.0%
GAAAAAACTTTAACCCTTCA+AGG + chr3.3:37874301-37874320 MS.gene29888:CDS 30.0%
! TTTGGAATTACACTATTTGG+TGG - chr3.3:37874532-37874551 None:intergenic 30.0%
AATCTTAACAACACAGATGG+TGG + chr3.3:37874550-37874569 MS.gene29888:CDS 35.0%
ACTAACAGAAACTGGTTAGT+TGG - chr3.3:37874190-37874209 None:intergenic 35.0%
ATCCCAATATGTGCATTGTT+AGG - chr3.3:37874658-37874677 None:intergenic 35.0%
CACGACCTATAATCGTATAA+GGG - chr3.3:37874375-37874394 None:intergenic 35.0%
CCAAATCTTAACAACACAGA+TGG + chr3.3:37874547-37874566 MS.gene29888:CDS 35.0%
GATCCTAACAATGCACATAT+TGG + chr3.3:37874652-37874671 MS.gene29888:CDS 35.0%
TGAAGATGACTAACAGAAAC+TGG - chr3.3:37874198-37874217 None:intergenic 35.0%
! CCATCTGTGTTGTTAAGATT+TGG - chr3.3:37874550-37874569 None:intergenic 35.0%
!! ACTGCTTTCAATCGATTTGT+TGG + chr3.3:37874598-37874617 MS.gene29888:CDS 35.0%
AATGACAGCATTCCCTTGAA+GGG - chr3.3:37874317-37874336 None:intergenic 40.0%
AGACCAATGCTAACTGTTTC+TGG - chr3.3:37874856-37874875 None:intergenic 40.0%
CAAATCAACGACTTGAGCAA+CGG - chr3.3:37874821-37874840 None:intergenic 40.0%
CTCAGTTACTAGCAAGAGAA+AGG - chr3.3:37874227-37874246 None:intergenic 40.0%
GACTTAGCTGATTACATCTC+TGG + chr3.3:37874277-37874296 MS.gene29888:CDS 40.0%
GATGTAATCAGCTAAGTCGA+AGG - chr3.3:37874275-37874294 None:intergenic 40.0%
GCACGACCTATAATCGTATA+AGG - chr3.3:37874376-37874395 None:intergenic 40.0%
GTTTCTCTCATTGACGAGAA+TGG + chr3.3:37874784-37874803 MS.gene29888:CDS 40.0%
TGTCATTCAGGAAACTCACT+TGG + chr3.3:37874330-37874349 MS.gene29888:CDS 40.0%
! GACCAATGCTAACTGTTTCT+GGG - chr3.3:37874855-37874874 None:intergenic 40.0%
!! ACAGTTAGCATTGGTCTATC+TGG + chr3.3:37874859-37874878 MS.gene29888:CDS 40.0%
!! TCTTTTTCTCTTGTGCCGTT+CGG + chr3.3:37874466-37874485 MS.gene29888:CDS 40.0%
!! TTGACGTTTTTCAACTGACG+TGG - chr3.3:37874887-37874906 None:intergenic 40.0%
AAGATAAGACCATCAGCTCG+TGG - chr3.3:37874499-37874518 None:intergenic 45.0%
ATTCAACACCCGTTCCCATT+TGG + chr3.3:37874401-37874420 MS.gene29888:CDS 45.0%
CCAGTTGCACTATCCCAAAT+GGG - chr3.3:37874418-37874437 None:intergenic 45.0%
CGGCACAAGAGAAAAAGAGA+AGG - chr3.3:37874464-37874483 None:intergenic 45.0%
CTCCCAGAAACAGTTAGCAT+TGG + chr3.3:37874850-37874869 MS.gene29888:CDS 45.0%
GAAACTCACTTGGAGCTTAC+TGG + chr3.3:37874340-37874359 MS.gene29888:CDS 45.0%
GAATGACAGCATTCCCTTGA+AGG - chr3.3:37874318-37874337 None:intergenic 45.0%
TCAAGGGAATGCTGTCATTC+AGG + chr3.3:37874318-37874337 MS.gene29888:CDS 45.0%
TCCCCATGGGATTCACAAAA+CGG + chr3.3:37874709-37874728 MS.gene29888:CDS 45.0%
TGCACTATCCCAAATGGGAA+CGG - chr3.3:37874413-37874432 None:intergenic 45.0%
TTCAACACCCGTTCCCATTT+GGG + chr3.3:37874402-37874421 MS.gene29888:CDS 45.0%
TTCATCCAAGACATCCCCAT+GGG + chr3.3:37874696-37874715 MS.gene29888:CDS 45.0%
TTTCATCCAAGACATCCCCA+TGG + chr3.3:37874695-37874714 MS.gene29888:CDS 45.0%
!! AACACAGATGGTGGAGATCT+GGG + chr3.3:37874559-37874578 MS.gene29888:CDS 45.0%
!! GGGAACGGGTGTTGAATATA+AGG - chr3.3:37874398-37874417 None:intergenic 45.0%
CCCATGGGATTCACAAAACG+GGG + chr3.3:37874711-37874730 MS.gene29888:CDS 50.0%
CCCCATGGGATTCACAAAAC+GGG + chr3.3:37874710-37874729 MS.gene29888:CDS 50.0%
GCACTATCCCAAATGGGAAC+GGG - chr3.3:37874412-37874431 None:intergenic 50.0%
GCCAGTTGCACTATCCCAAA+TGG - chr3.3:37874419-37874438 None:intergenic 50.0%
GCTCGTGGATAACTACCGAA+CGG - chr3.3:37874484-37874503 None:intergenic 50.0%
GGTAGTTATCCACGAGCTGA+TGG + chr3.3:37874487-37874506 MS.gene29888:CDS 50.0%
TGAATCCCATGGGGATGTCT+TGG - chr3.3:37874704-37874723 None:intergenic 50.0%
! ACCCCGTTTTGTGAATCCCA+TGG - chr3.3:37874715-37874734 None:intergenic 50.0%
! CCCATTTGGGATAGTGCAAC+TGG + chr3.3:37874415-37874434 MS.gene29888:CDS 50.0%
! CCCCGTTTTGTGAATCCCAT+GGG - chr3.3:37874714-37874733 None:intergenic 50.0%
! CCCGTTTTGTGAATCCCATG+GGG - chr3.3:37874713-37874732 None:intergenic 50.0%
!! CAACACAGATGGTGGAGATC+TGG + chr3.3:37874558-37874577 MS.gene29888:CDS 50.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 37874175 37874999 37874175 ID=MS.gene29888
chr3.3 mRNA 37874175 37874999 37874175 ID=MS.gene29888.t1;Parent=MS.gene29888
chr3.3 exon 37874175 37874999 37874175 ID=MS.gene29888.t1.exon1;Parent=MS.gene29888.t1
chr3.3 CDS 37874175 37874999 37874175 ID=cds.MS.gene29888.t1;Parent=MS.gene29888.t1
Gene Sequence

>MS.gene29888

ATGGCTTTCTTTCCAACTAACCAGTTTCTGTTAGTCATCTTCACAACTTCCTTTCTCTTGCTAGTAACTGAGTTTGTGAACTCAGACGAAACAGTTTCCTTCGACTTAGCTGATTACATCTCTGGTGAAAAAACTTTAACCCTTCAAGGGAATGCTGTCATTCAGGAAACTCACTTGGAGCTTACTGGTGACATTAACCCTTATACGATTATAGGTCGTGCCTTATATTCAACACCCGTTCCCATTTGGGATAGTGCAACTGGCAATGTCGCTAGTTTTGTGACTTCCTTCTCTTTTTCTCTTGTGCCGTTCGGTAGTTATCCACGAGCTGATGGTCTTATCTTCTTTCTTGCACCACCAAATAGTGTAATTCCAAATCTTAACAACACAGATGGTGGAGATCTGGGAGTAGTCGATGAAACAACTGCTTTCAATCGATTTGTTGGTGTAGAATTTGATAATTATCACAATGATTGGGATCCTAACAATGCACATATTGGGATCGATGTCAACTCTTTAATTTCATCCAAGACATCCCCATGGGATTCACAAAACGGGGTGTTGTACAATGTTAGTATCATCTATGATTCTTCTTCTAAGACTTTGAGTGTTTCTCTCATTGACGAGAATGGTCAACTTTCTACCGTTGCTCAAGTCGTTGATTTGAAAGCTGTGCTCCCAGAAACAGTTAGCATTGGTCTATCTGGTTCCACGTCAGTTGAAAAACGTCAAATATATGTCATTGAAACATGGTCTTTCAAGTCAATTTTGAAGACAACTATAAGCAGCAGCACCTCAGAGAACATAAATCATACTGCAATCTAG

Protein sequence

>MS.gene29888.t1

MAFFPTNQFLLVIFTTSFLLLVTEFVNSDETVSFDLADYISGEKTLTLQGNAVIQETHLELTGDINPYTIIGRALYSTPVPIWDSATGNVASFVTSFSFSLVPFGSYPRADGLIFFLAPPNSVIPNLNNTDGGDLGVVDETTAFNRFVGVEFDNYHNDWDPNNAHIGIDVNSLISSKTSPWDSQNGVLYNVSIIYDSSSKTLSVSLIDENGQLSTVAQVVDLKAVLPETVSIGLSGSTSVEKRQIYVIETWSFKSILKTTISSSTSENINHTAI