Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29957.t1 | XP_003598051.1 | 100 | 172 | 0 | 0 | 1 | 172 | 127 | 298 | 4.50E-98 | 367.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29957.t1 | Q9LQ32 | 70.3 | 172 | 50 | 1 | 1 | 172 | 120 | 290 | 4.8e-72 | 271.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29957.t1 | G7J059 | 100.0 | 172 | 0 | 0 | 1 | 172 | 127 | 298 | 3.3e-98 | 367.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049227 | MS.gene29957 | 0.840279 | 9.57E-58 | -1.69E-46 |
| MS.gene049418 | MS.gene29957 | 0.8023 | 5.99E-49 | -1.69E-46 |
| MS.gene052732 | MS.gene29957 | 0.803445 | 3.47E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene29957 | 0.873719 | 1.18E-67 | -1.69E-46 |
| MS.gene054584 | MS.gene29957 | 0.862464 | 4.98E-64 | -1.69E-46 |
| MS.gene058206 | MS.gene29957 | 0.812112 | 4.99E-51 | -1.69E-46 |
| MS.gene058722 | MS.gene29957 | 0.821397 | 4.13E-53 | -1.69E-46 |
| MS.gene058945 | MS.gene29957 | 0.805113 | 1.56E-49 | -1.69E-46 |
| MS.gene060884 | MS.gene29957 | 0.817227 | 3.68E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene29957 | 0.816558 | 5.20E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29957.t1 | MTR_3g005710 | 100.000 | 172 | 0 | 0 | 1 | 172 | 127 | 298 | 4.02e-132 | 371 |
| MS.gene29957.t1 | MTR_8g080660 | 43.094 | 181 | 93 | 3 | 1 | 171 | 117 | 297 | 3.40e-48 | 158 |
| MS.gene29957.t1 | MTR_4g485560 | 45.399 | 163 | 87 | 1 | 1 | 161 | 70 | 232 | 1.75e-46 | 152 |
| MS.gene29957.t1 | MTR_4g485570 | 43.558 | 163 | 90 | 1 | 1 | 161 | 120 | 282 | 5.75e-45 | 150 |
| MS.gene29957.t1 | MTR_3g085270 | 43.827 | 162 | 88 | 2 | 1 | 159 | 113 | 274 | 3.32e-44 | 147 |
| MS.gene29957.t1 | MTR_4g094895 | 39.752 | 161 | 93 | 2 | 1 | 157 | 123 | 283 | 5.46e-38 | 132 |
| MS.gene29957.t1 | MTR_7g029100 | 42.857 | 161 | 75 | 3 | 1 | 160 | 122 | 266 | 5.01e-37 | 129 |
| MS.gene29957.t1 | MTR_4g102900 | 38.286 | 175 | 101 | 5 | 1 | 172 | 127 | 297 | 9.23e-34 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene29957.t1 | AT1G33800 | 70.349 | 172 | 50 | 1 | 1 | 172 | 120 | 290 | 7.24e-93 | 272 |
| MS.gene29957.t1 | AT4G09990 | 70.520 | 173 | 49 | 2 | 1 | 172 | 112 | 283 | 2.91e-92 | 270 |
| MS.gene29957.t1 | AT1G09610 | 65.537 | 177 | 55 | 3 | 1 | 172 | 107 | 282 | 1.55e-87 | 258 |
| MS.gene29957.t1 | AT1G71690 | 60.452 | 177 | 65 | 3 | 1 | 172 | 119 | 295 | 6.00e-75 | 226 |
| MS.gene29957.t1 | AT1G67330 | 47.977 | 173 | 86 | 3 | 1 | 169 | 114 | 286 | 8.74e-52 | 167 |
| MS.gene29957.t1 | AT1G27930 | 47.305 | 167 | 85 | 2 | 1 | 164 | 111 | 277 | 1.50e-49 | 161 |
| MS.gene29957.t1 | AT2G15440 | 36.723 | 177 | 106 | 2 | 1 | 171 | 127 | 303 | 5.47e-40 | 138 |
| MS.gene29957.t1 | AT3G50220 | 40.373 | 161 | 92 | 2 | 1 | 157 | 137 | 297 | 1.35e-36 | 129 |
| MS.gene29957.t1 | AT5G67210 | 40.373 | 161 | 92 | 2 | 1 | 157 | 130 | 290 | 3.00e-36 | 128 |
| MS.gene29957.t1 | AT4G24910 | 34.524 | 168 | 99 | 4 | 1 | 159 | 132 | 297 | 1.39e-21 | 89.4 |
Find 48 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGTGGATTAGCACTTAA+AGG | 0.223364 | 3.4:-1507670 | MS.gene29957:CDS |
| AGTGATGAACTAATGAAAAT+TGG | 0.261741 | 3.4:-1507739 | MS.gene29957:CDS |
| AAATCAATTTCATGGACAAT+AGG | 0.306327 | 3.4:+1507366 | None:intergenic |
| ATTGCTAGGAATAGAGAAAA+TGG | 0.307486 | 3.4:-1507535 | MS.gene29957:CDS |
| CCAACGTTGGGAACTTTGAT+TGG | 0.316396 | 3.4:+1507800 | None:intergenic |
| ACCAAGTCATAGGGCACGTT+TGG | 0.318589 | 3.4:-1507395 | MS.gene29957:CDS |
| TCCATGTCTTGTCTTCTTCC+AGG | 0.323320 | 3.4:+1507833 | None:intergenic |
| GGGTACTTCGATGGCGCGCC+GGG | 0.332994 | 3.4:-1507589 | MS.gene29957:CDS |
| ACTTCAATATACCAAGTCAT+AGG | 0.375565 | 3.4:-1507405 | MS.gene29957:CDS |
| ATACAGCTGGTTTGATTGCT+AGG | 0.379409 | 3.4:-1507549 | MS.gene29957:CDS |
| AATCAATTTCATGGACAATA+GGG | 0.382464 | 3.4:+1507367 | None:intergenic |
| TGTTTGTTCATGATGTTGAT+AGG | 0.396580 | 3.4:-1507501 | MS.gene29957:CDS |
| ATGAGTGCTATTTATACAGC+TGG | 0.441985 | 3.4:-1507562 | MS.gene29957:CDS |
| CTTGTCTTCTTCCAGGAAAA+CGG | 0.456850 | 3.4:+1507840 | None:intergenic |
| TTCTTTGTGAAGGTTATTTG+AGG | 0.465004 | 3.4:-1507450 | MS.gene29957:CDS |
| CCAAGTCATAGGGCACGTTT+GGG | 0.466887 | 3.4:-1507394 | MS.gene29957:CDS |
| TCTTTGTGAAGGTTATTTGA+GGG | 0.475343 | 3.4:-1507449 | MS.gene29957:CDS |
| GGGGTACTTCGATGGCGCGC+CGG | 0.481478 | 3.4:-1507590 | MS.gene29957:CDS |
| CCCAAACGTGCCCTATGACT+TGG | 0.483562 | 3.4:+1507394 | None:intergenic |
| ATCATGGTGGATGCACCAAC+GGG | 0.492498 | 3.4:-1507610 | MS.gene29957:CDS |
| CATGATATGATTCCAACGTT+GGG | 0.499455 | 3.4:+1507788 | None:intergenic |
| GGGAACAAGAAGGAAGGATT+AGG | 0.499937 | 3.4:-1507429 | MS.gene29957:CDS |
| TGAAATTTATGATGTGGAAT+GGG | 0.503077 | 3.4:-1507638 | MS.gene29957:CDS |
| TCCACATCATAAATTTCACT+TGG | 0.503817 | 3.4:+1507643 | None:intergenic |
| TCCAAGTGAAATTTATGATG+TGG | 0.511674 | 3.4:-1507644 | MS.gene29957:CDS |
| GTGAAATTTATGATGTGGAA+TGG | 0.519138 | 3.4:-1507639 | MS.gene29957:CDS |
| GCACCAACGGGGTACTTCGA+TGG | 0.521410 | 3.4:-1507598 | MS.gene29957:CDS |
| TGTGGAATGGGATGTGATCA+TGG | 0.525871 | 3.4:-1507626 | MS.gene29957:CDS |
| CATCATTGAACTACGGTGGC+CGG | 0.528792 | 3.4:-1507864 | MS.gene29957:CDS |
| CTTCTTCCAGGAAAACGGTC+CGG | 0.530964 | 3.4:+1507845 | None:intergenic |
| ACATGATATGATTCCAACGT+TGG | 0.536855 | 3.4:+1507787 | None:intergenic |
| ATTTGAGGGAACAAGAAGGA+AGG | 0.541340 | 3.4:-1507435 | MS.gene29957:CDS |
| ACTTCGATGGCGCGCCGGGG+AGG | 0.549464 | 3.4:-1507585 | MS.gene29957:CDS |
| GAAAATTGGAATGGAACAAG+AGG | 0.553174 | 3.4:-1507725 | MS.gene29957:CDS |
| TCCTGGAAGAAGACAAGACA+TGG | 0.567326 | 3.4:-1507834 | MS.gene29957:CDS |
| TGAACTAATGAAAATTGGAA+TGG | 0.575241 | 3.4:-1507734 | MS.gene29957:CDS |
| CCAATCAAAGTTCCCAACGT+TGG | 0.578373 | 3.4:-1507800 | MS.gene29957:CDS |
| CTTCAATATACCAAGTCATA+GGG | 0.581013 | 3.4:-1507404 | MS.gene29957:CDS |
| GGTTATTTGAGGGAACAAGA+AGG | 0.586352 | 3.4:-1507439 | MS.gene29957:CDS |
| TGATGTTGATAGGAAAGTTG+AGG | 0.590781 | 3.4:-1507491 | MS.gene29957:CDS |
| GATCATGGTGGATGCACCAA+CGG | 0.605400 | 3.4:-1507611 | MS.gene29957:CDS |
| GCGCCATCGAAGTACCCCGT+TGG | 0.633771 | 3.4:+1507595 | None:intergenic |
| TAAATAGCACTCATCCTCCC+CGG | 0.635229 | 3.4:+1507571 | None:intergenic |
| ATGTGGACATCATTGAACTA+CGG | 0.637354 | 3.4:-1507871 | MS.gene29957:CDS |
| TGGACATCATTGAACTACGG+TGG | 0.678674 | 3.4:-1507868 | MS.gene29957:CDS |
| GGTACTTCGATGGCGCGCCG+GGG | 0.680331 | 3.4:-1507588 | MS.gene29957:CDS |
| GGAATGGGATGTGATCATGG+TGG | 0.725748 | 3.4:-1507623 | MS.gene29957:CDS |
| TCATGGTGGATGCACCAACG+GGG | 0.763267 | 3.4:-1507609 | MS.gene29957:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGTGATGAACTAATGAAAAT+TGG | - | chr3.4:1507507-1507526 | MS.gene29957:CDS | 25.0% |
| ! | TGAAATTTATGATGTGGAAT+GGG | - | chr3.4:1507608-1507627 | MS.gene29957:CDS | 25.0% |
| ! | TGAACTAATGAAAATTGGAA+TGG | - | chr3.4:1507512-1507531 | MS.gene29957:CDS | 25.0% |
| !!! | AATCCACATTTTGAAAATCT+TGG | + | chr3.4:1507567-1507586 | None:intergenic | 25.0% |
| !!! | ATCCACATTTTGAAAATCTT+GGG | + | chr3.4:1507566-1507585 | None:intergenic | 25.0% |
| ACTTCAATATACCAAGTCAT+AGG | - | chr3.4:1507841-1507860 | MS.gene29957:CDS | 30.0% | |
| ATTGCTAGGAATAGAGAAAA+TGG | - | chr3.4:1507711-1507730 | MS.gene29957:CDS | 30.0% | |
| CTTCAATATACCAAGTCATA+GGG | - | chr3.4:1507842-1507861 | MS.gene29957:CDS | 30.0% | |
| GTGAAATTTATGATGTGGAA+TGG | - | chr3.4:1507607-1507626 | MS.gene29957:CDS | 30.0% | |
| TCCAAGTGAAATTTATGATG+TGG | - | chr3.4:1507602-1507621 | MS.gene29957:CDS | 30.0% | |
| TCCACATCATAAATTTCACT+TGG | + | chr3.4:1507606-1507625 | None:intergenic | 30.0% | |
| TCTTTGTGAAGGTTATTTGA+GGG | - | chr3.4:1507797-1507816 | MS.gene29957:CDS | 30.0% | |
| TGTTTGTTCATGATGTTGAT+AGG | - | chr3.4:1507745-1507764 | MS.gene29957:CDS | 30.0% | |
| TTCTTTGTGAAGGTTATTTG+AGG | - | chr3.4:1507796-1507815 | MS.gene29957:CDS | 30.0% | |
| ! | AGTTGAGGATCAATTTTCAA+AGG | - | chr3.4:1507770-1507789 | MS.gene29957:CDS | 30.0% |
| !! | AAATGTGGATTAGCACTTAA+AGG | - | chr3.4:1507576-1507595 | MS.gene29957:CDS | 30.0% |
| ACATGATATGATTCCAACGT+TGG | + | chr3.4:1507462-1507481 | None:intergenic | 35.0% | |
| ATGTGGACATCATTGAACTA+CGG | - | chr3.4:1507375-1507394 | MS.gene29957:CDS | 35.0% | |
| CATGATATGATTCCAACGTT+GGG | + | chr3.4:1507461-1507480 | None:intergenic | 35.0% | |
| GAAAATTGGAATGGAACAAG+AGG | - | chr3.4:1507521-1507540 | MS.gene29957:CDS | 35.0% | |
| ! | GACCCAAGATTTTCAAAATG+TGG | - | chr3.4:1507561-1507580 | MS.gene29957:CDS | 35.0% |
| ! | TCAAAGGCTTTTCTTTGTGA+AGG | - | chr3.4:1507786-1507805 | MS.gene29957:CDS | 35.0% |
| ! | TGATGTTGATAGGAAAGTTG+AGG | - | chr3.4:1507755-1507774 | MS.gene29957:CDS | 35.0% |
| !! | ATGAGTGCTATTTATACAGC+TGG | - | chr3.4:1507684-1507703 | MS.gene29957:CDS | 35.0% |
| ATTTGAGGGAACAAGAAGGA+AGG | - | chr3.4:1507811-1507830 | MS.gene29957:CDS | 40.0% | |
| CTTGTCTTCTTCCAGGAAAA+CGG | + | chr3.4:1507409-1507428 | None:intergenic | 40.0% | |
| GGTTATTTGAGGGAACAAGA+AGG | - | chr3.4:1507807-1507826 | MS.gene29957:CDS | 40.0% | |
| !! | ATACAGCTGGTTTGATTGCT+AGG | - | chr3.4:1507697-1507716 | MS.gene29957:CDS | 40.0% |
| CCAACGTTGGGAACTTTGAT+TGG | + | chr3.4:1507449-1507468 | None:intergenic | 45.0% | |
| CCAATCAAAGTTCCCAACGT+TGG | - | chr3.4:1507446-1507465 | MS.gene29957:CDS | 45.0% | |
| GGGAACAAGAAGGAAGGATT+AGG | - | chr3.4:1507817-1507836 | MS.gene29957:CDS | 45.0% | |
| TAAATAGCACTCATCCTCCC+CGG | + | chr3.4:1507678-1507697 | None:intergenic | 45.0% | |
| TCCATGTCTTGTCTTCTTCC+AGG | + | chr3.4:1507416-1507435 | None:intergenic | 45.0% | |
| TCCTGGAAGAAGACAAGACA+TGG | - | chr3.4:1507412-1507431 | MS.gene29957:CDS | 45.0% | |
| TGGACATCATTGAACTACGG+TGG | - | chr3.4:1507378-1507397 | MS.gene29957:CDS | 45.0% | |
| TGTGGAATGGGATGTGATCA+TGG | - | chr3.4:1507620-1507639 | MS.gene29957:CDS | 45.0% | |
| ACCAAGTCATAGGGCACGTT+TGG | - | chr3.4:1507851-1507870 | MS.gene29957:CDS | 50.0% | |
| CATCATTGAACTACGGTGGC+CGG | - | chr3.4:1507382-1507401 | MS.gene29957:CDS | 50.0% | |
| CCAAGTCATAGGGCACGTTT+GGG | - | chr3.4:1507852-1507871 | MS.gene29957:CDS | 50.0% | |
| CTTCTTCCAGGAAAACGGTC+CGG | + | chr3.4:1507404-1507423 | None:intergenic | 50.0% | |
| GGAATGGGATGTGATCATGG+TGG | - | chr3.4:1507623-1507642 | MS.gene29957:CDS | 50.0% | |
| !! | ATCATGGTGGATGCACCAAC+GGG | - | chr3.4:1507636-1507655 | MS.gene29957:CDS | 50.0% |
| !! | GATCATGGTGGATGCACCAA+CGG | - | chr3.4:1507635-1507654 | MS.gene29957:CDS | 50.0% |
| CCCAAACGTGCCCTATGACT+TGG | + | chr3.4:1507855-1507874 | None:intergenic | 55.0% | |
| !! | TCATGGTGGATGCACCAACG+GGG | - | chr3.4:1507637-1507656 | MS.gene29957:CDS | 55.0% |
| ! | GCACCAACGGGGTACTTCGA+TGG | - | chr3.4:1507648-1507667 | MS.gene29957:CDS | 60.0% |
| ! | GCGCCATCGAAGTACCCCGT+TGG | + | chr3.4:1507654-1507673 | None:intergenic | 65.0% |
| GGGTACTTCGATGGCGCGCC+GGG | - | chr3.4:1507657-1507676 | MS.gene29957:CDS | 70.0% | |
| GGTACTTCGATGGCGCGCCG+GGG | - | chr3.4:1507658-1507677 | MS.gene29957:CDS | 70.0% | |
| ! | CGGTGGCCGGACCGTTTTCC+TGG | - | chr3.4:1507395-1507414 | MS.gene29957:CDS | 70.0% |
| ! | GGGGTACTTCGATGGCGCGC+CGG | - | chr3.4:1507656-1507675 | MS.gene29957:CDS | 70.0% |
| ACTTCGATGGCGCGCCGGGG+AGG | - | chr3.4:1507661-1507680 | MS.gene29957:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 1507375 | 1507893 | 1507375 | ID=MS.gene29957 |
| chr3.4 | mRNA | 1507375 | 1507893 | 1507375 | ID=MS.gene29957.t1;Parent=MS.gene29957 |
| chr3.4 | exon | 1507375 | 1507893 | 1507375 | ID=MS.gene29957.t1.exon1;Parent=MS.gene29957.t1 |
| chr3.4 | CDS | 1507375 | 1507893 | 1507375 | ID=cds.MS.gene29957.t1;Parent=MS.gene29957.t1 |
| Gene Sequence |
| Protein sequence |
