Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060886.t1 | RHN76992.1 | 98.3 | 301 | 4 | 1 | 1 | 301 | 261 | 560 | 1.20E-133 | 486.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060886.t1 | Q9C7G1 | 32.2 | 183 | 116 | 3 | 18 | 198 | 527 | 703 | 8.2e-19 | 95.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060886.t1 | G7I8B4 | 98.3 | 301 | 4 | 1 | 1 | 301 | 216 | 515 | 8.4e-134 | 486.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05157 | MS.gene060886 | 0.800303 | 1.54E-48 | -1.69E-46 |
| MS.gene051719 | MS.gene060886 | 0.810343 | 1.21E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene060886 | 0.806335 | 8.65E-50 | -1.69E-46 |
| MS.gene052732 | MS.gene060886 | 0.820641 | 6.16E-53 | -1.69E-46 |
| MS.gene053144 | MS.gene060886 | 0.883806 | 3.29E-71 | -1.69E-46 |
| MS.gene053581 | MS.gene060886 | 0.822833 | 1.92E-53 | -1.69E-46 |
| MS.gene054584 | MS.gene060886 | 0.864237 | 1.40E-64 | -1.69E-46 |
| MS.gene055537 | MS.gene060886 | 0.801009 | 1.10E-48 | -1.69E-46 |
| MS.gene055580 | MS.gene060886 | -0.803778 | 2.96E-49 | -1.69E-46 |
| MS.gene059154 | MS.gene060886 | 0.803723 | 3.04E-49 | -1.69E-46 |
| MS.gene059297 | MS.gene060886 | 0.807432 | 5.07E-50 | -1.69E-46 |
| MS.gene060587 | MS.gene060886 | 0.802857 | 4.60E-49 | -1.69E-46 |
| MS.gene060884 | MS.gene060886 | 0.989303 | 1.52E-177 | -1.69E-46 |
| MS.gene060886 | MS.gene060888 | 0.882412 | 1.06E-70 | -1.69E-46 |
| MS.gene060886 | MS.gene061840 | 0.856811 | 2.50E-62 | -1.69E-46 |
| MS.gene060886 | MS.gene062043 | 0.816598 | 5.09E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene062821 | 0.831642 | 1.49E-55 | -1.69E-46 |
| MS.gene060886 | MS.gene063452 | 0.843752 | 1.16E-58 | -1.69E-46 |
| MS.gene060886 | MS.gene065667 | 0.822621 | 2.15E-53 | -1.69E-46 |
| MS.gene060886 | MS.gene066105 | 0.801495 | 8.76E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene067071 | 0.830483 | 2.86E-55 | -1.69E-46 |
| MS.gene060886 | MS.gene068022 | 0.819851 | 9.35E-53 | -1.69E-46 |
| MS.gene060886 | MS.gene068925 | 0.83642 | 9.46E-57 | -1.69E-46 |
| MS.gene060886 | MS.gene071315 | 0.838098 | 3.52E-57 | -1.69E-46 |
| MS.gene060886 | MS.gene071316 | 0.836272 | 1.03E-56 | -1.69E-46 |
| MS.gene060886 | MS.gene072115 | 0.829431 | 5.17E-55 | -1.69E-46 |
| MS.gene060886 | MS.gene07359 | 0.849693 | 2.75E-60 | -1.69E-46 |
| MS.gene060886 | MS.gene074337 | 0.815393 | 9.46E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene08581 | 0.812981 | 3.23E-51 | -1.69E-46 |
| MS.gene060886 | MS.gene09087 | 0.820899 | 5.37E-53 | -1.69E-46 |
| MS.gene060886 | MS.gene09947 | 0.802286 | 6.03E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene20903 | 0.854869 | 9.23E-62 | -1.69E-46 |
| MS.gene060886 | MS.gene21144 | 0.816476 | 5.42E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene25810 | 0.83065 | 2.61E-55 | -1.69E-46 |
| MS.gene060886 | MS.gene26659 | 0.81599 | 6.96E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene27918 | 0.805743 | 1.15E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene28387 | 0.816585 | 5.13E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene29755 | 0.805809 | 1.12E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene29957 | 0.816558 | 5.20E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene30882 | 0.824711 | 6.96E-54 | -1.69E-46 |
| MS.gene060886 | MS.gene31203 | 0.832774 | 7.80E-56 | -1.69E-46 |
| MS.gene060886 | MS.gene31204 | 0.835266 | 1.85E-56 | -1.69E-46 |
| MS.gene060886 | MS.gene31433 | 0.804007 | 2.66E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene33141 | 0.869398 | 3.19E-66 | -1.69E-46 |
| MS.gene060886 | MS.gene34286 | 0.876175 | 1.72E-68 | -1.69E-46 |
| MS.gene060886 | MS.gene34420 | 0.818349 | 2.05E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene35718 | 0.82414 | 9.48E-54 | -1.69E-46 |
| MS.gene060886 | MS.gene36284 | 0.82673 | 2.31E-54 | -1.69E-46 |
| MS.gene060886 | MS.gene37670 | 0.819276 | 1.26E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene38303 | 0.869255 | 3.55E-66 | -1.69E-46 |
| MS.gene060886 | MS.gene38561 | 0.833818 | 4.29E-56 | -1.69E-46 |
| MS.gene060886 | MS.gene38693 | 0.809199 | 2.13E-50 | -1.69E-46 |
| MS.gene060886 | MS.gene40409 | 0.800388 | 1.48E-48 | -1.69E-46 |
| MS.gene060886 | MS.gene41679 | 0.803248 | 3.82E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene43178 | 0.801113 | 1.05E-48 | -1.69E-46 |
| MS.gene060886 | MS.gene43740 | 0.837306 | 5.62E-57 | -1.69E-46 |
| MS.gene060886 | MS.gene44783 | 0.813981 | 1.94E-51 | -1.69E-46 |
| MS.gene060886 | MS.gene44844 | 0.841029 | 6.09E-58 | -1.69E-46 |
| MS.gene060886 | MS.gene47875 | 0.81565 | 8.29E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene48814 | 0.808494 | 3.01E-50 | -1.69E-46 |
| MS.gene060886 | MS.gene49359 | 0.811689 | 6.17E-51 | -1.69E-46 |
| MS.gene060886 | MS.gene49559 | 0.800213 | 1.60E-48 | -1.69E-46 |
| MS.gene060886 | MS.gene50564 | 0.818629 | 1.77E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene52057 | 0.822716 | 2.04E-53 | -1.69E-46 |
| MS.gene060886 | MS.gene53249 | 0.809464 | 1.87E-50 | -1.69E-46 |
| MS.gene060886 | MS.gene53280 | 0.802843 | 4.63E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene58214 | 0.806547 | 7.80E-50 | -1.69E-46 |
| MS.gene060886 | MS.gene61627 | 0.825305 | 5.04E-54 | -1.69E-46 |
| MS.gene060886 | MS.gene62916 | 0.815171 | 1.06E-51 | -1.69E-46 |
| MS.gene060886 | MS.gene65276 | 0.847064 | 1.46E-59 | -1.69E-46 |
| MS.gene060886 | MS.gene70730 | 0.828825 | 7.25E-55 | -1.69E-46 |
| MS.gene060886 | MS.gene71704 | 0.818235 | 2.18E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene72135 | 0.814209 | 1.73E-51 | -1.69E-46 |
| MS.gene060886 | MS.gene72707 | 0.836209 | 1.07E-56 | -1.69E-46 |
| MS.gene060886 | MS.gene75538 | 0.804869 | 1.76E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene76032 | 0.806718 | 7.18E-50 | -1.69E-46 |
| MS.gene060886 | MS.gene78729 | 0.802836 | 4.64E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene79900 | 0.804364 | 2.24E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene81678 | 0.806415 | 8.32E-50 | -1.69E-46 |
| MS.gene060886 | MS.gene84426 | 0.808495 | 3.01E-50 | -1.69E-46 |
| MS.gene060886 | MS.gene88718 | 0.810077 | 1.38E-50 | -1.69E-46 |
| MS.gene060886 | MS.gene89099 | 0.830988 | 2.15E-55 | -1.69E-46 |
| MS.gene060886 | MS.gene89974 | 0.827138 | 1.85E-54 | -1.69E-46 |
| MS.gene060886 | MS.gene95438 | 0.800155 | 1.65E-48 | -1.69E-46 |
| MS.gene060886 | MS.gene97156 | 0.817618 | 3.00E-52 | -1.69E-46 |
| MS.gene060886 | MS.gene97306 | 0.806386 | 8.44E-50 | -1.69E-46 |
| MS.gene060886 | MS.gene97761 | 0.821392 | 4.14E-53 | -1.69E-46 |
| MS.gene060886 | MS.gene97762 | 0.834989 | 2.18E-56 | -1.69E-46 |
| MS.gene060886 | MS.gene98395 | 0.814015 | 1.91E-51 | -1.69E-46 |
| MS.gene060886 | MS.gene99224 | 0.80262 | 5.15E-49 | -1.69E-46 |
| MS.gene060886 | MS.gene99595 | 0.80849 | 3.02E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060886.t1 | MTR_1g014420 | 98.007 | 301 | 5 | 1 | 1 | 301 | 216 | 515 | 0.0 | 597 |
| MS.gene060886.t1 | MTR_3g106140 | 74.503 | 302 | 73 | 2 | 1 | 301 | 197 | 495 | 3.76e-142 | 410 |
| MS.gene060886.t1 | MTR_2g015870 | 35.433 | 254 | 132 | 7 | 52 | 301 | 215 | 440 | 2.37e-30 | 119 |
| MS.gene060886.t1 | MTR_8g080280 | 31.868 | 182 | 114 | 3 | 21 | 198 | 534 | 709 | 2.77e-22 | 97.4 |
| MS.gene060886.t1 | MTR_5g079550 | 28.253 | 269 | 156 | 7 | 15 | 275 | 116 | 355 | 8.71e-20 | 89.0 |
| MS.gene060886.t1 | MTR_3g085610 | 28.230 | 209 | 140 | 4 | 1 | 206 | 514 | 715 | 4.33e-17 | 82.0 |
| MS.gene060886.t1 | MTR_3g067685 | 27.413 | 259 | 153 | 8 | 32 | 285 | 154 | 382 | 1.93e-16 | 79.3 |
| MS.gene060886.t1 | MTR_4g063800 | 32.990 | 194 | 113 | 7 | 17 | 204 | 452 | 634 | 2.41e-16 | 79.7 |
| MS.gene060886.t1 | MTR_4g485520 | 30.978 | 184 | 117 | 4 | 17 | 198 | 472 | 647 | 2.65e-14 | 73.6 |
| MS.gene060886.t1 | MTR_4g485520 | 30.978 | 184 | 117 | 4 | 17 | 198 | 304 | 479 | 3.89e-14 | 72.8 |
| MS.gene060886.t1 | MTR_7g059405 | 29.570 | 186 | 123 | 3 | 14 | 199 | 457 | 634 | 2.46e-11 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene060886.t1 | AT4G31890 | 66.113 | 301 | 87 | 5 | 1 | 301 | 233 | 518 | 2.77e-124 | 365 |
| MS.gene060886.t1 | AT4G31890 | 66.113 | 301 | 87 | 5 | 1 | 301 | 233 | 518 | 2.77e-124 | 365 |
| MS.gene060886.t1 | AT4G31890 | 66.113 | 301 | 87 | 5 | 1 | 301 | 233 | 518 | 2.77e-124 | 365 |
| MS.gene060886.t1 | AT2G25130 | 62.925 | 294 | 80 | 7 | 1 | 291 | 200 | 467 | 5.25e-114 | 337 |
| MS.gene060886.t1 | AT2G27430 | 32.707 | 266 | 154 | 5 | 36 | 301 | 198 | 438 | 6.47e-33 | 126 |
| MS.gene060886.t1 | AT1G67530 | 31.707 | 205 | 126 | 4 | 3 | 204 | 519 | 712 | 6.29e-21 | 93.6 |
| MS.gene060886.t1 | AT1G67530 | 31.707 | 205 | 126 | 4 | 3 | 204 | 519 | 712 | 6.29e-21 | 93.6 |
| MS.gene060886.t1 | AT1G67530 | 31.707 | 205 | 126 | 4 | 3 | 204 | 519 | 712 | 6.29e-21 | 93.6 |
| MS.gene060886.t1 | AT1G24330 | 31.707 | 205 | 131 | 4 | 20 | 221 | 531 | 729 | 1.68e-20 | 92.0 |
| MS.gene060886.t1 | AT1G24330 | 31.707 | 205 | 131 | 4 | 20 | 221 | 531 | 729 | 1.68e-20 | 92.0 |
| MS.gene060886.t1 | AT1G27910 | 33.333 | 183 | 114 | 4 | 18 | 198 | 527 | 703 | 1.76e-18 | 86.3 |
| MS.gene060886.t1 | AT2G28830 | 34.848 | 198 | 111 | 8 | 17 | 207 | 386 | 572 | 2.30e-16 | 79.7 |
| MS.gene060886.t1 | AT3G03440 | 26.894 | 264 | 156 | 7 | 21 | 276 | 169 | 403 | 1.43e-13 | 70.9 |
| MS.gene060886.t1 | AT3G46510 | 32.258 | 186 | 110 | 6 | 17 | 197 | 453 | 627 | 1.58e-13 | 71.2 |
| MS.gene060886.t1 | AT1G71020 | 31.892 | 185 | 115 | 4 | 17 | 198 | 295 | 471 | 2.15e-13 | 70.5 |
| MS.gene060886.t1 | AT1G71020 | 31.892 | 185 | 115 | 4 | 17 | 198 | 443 | 619 | 2.29e-13 | 70.9 |
| MS.gene060886.t1 | AT2G23140 | 31.016 | 187 | 120 | 4 | 13 | 198 | 449 | 627 | 8.96e-12 | 65.9 |
| MS.gene060886.t1 | AT2G23140 | 31.016 | 187 | 120 | 4 | 13 | 198 | 449 | 627 | 8.96e-12 | 65.9 |
| MS.gene060886.t1 | AT2G23140 | 31.016 | 187 | 120 | 4 | 13 | 198 | 638 | 816 | 1.39e-11 | 65.5 |
| MS.gene060886.t1 | AT2G23140 | 31.016 | 187 | 120 | 4 | 13 | 198 | 638 | 816 | 1.39e-11 | 65.5 |
| MS.gene060886.t1 | AT2G23140 | 31.016 | 187 | 120 | 4 | 13 | 198 | 641 | 819 | 1.41e-11 | 65.5 |
Find 65 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTCGAATAAACCGATAAT+TGG | 0.146060 | 1.2:+8254461 | MS.gene060886:CDS |
| AGCCAATGTTCAGTTTATTT+TGG | 0.151005 | 1.2:+8254604 | MS.gene060886:CDS |
| CTTCTTGAGTTGTCGCTTAT+TGG | 0.172434 | 1.2:+8254896 | MS.gene060886:CDS |
| CTGAAGAGTTCTAACTAAAA+AGG | 0.175660 | 1.2:-8254501 | None:intergenic |
| CCTTTCAATCCTTAGCAATT+TGG | 0.178700 | 1.2:+8254688 | MS.gene060886:CDS |
| GACGATGAAGTTCCGCAAAT+TGG | 0.211163 | 1.2:-8255031 | None:intergenic |
| CCCGGATGTGTTTCCTATCT+TGG | 0.249308 | 1.2:+8254748 | MS.gene060886:CDS |
| GGGAACTACAACGGAAATTT+AGG | 0.256409 | 1.2:+8254992 | MS.gene060886:CDS |
| GATAAAGGGAAACAGGTTTC+AGG | 0.257229 | 1.2:+8254971 | MS.gene060886:CDS |
| GAGAATAGATAAAGGGAAAC+AGG | 0.320824 | 1.2:+8254964 | MS.gene060886:CDS |
| GAAACGGGCGTCGAGGCTAT+TGG | 0.335825 | 1.2:+8254934 | MS.gene060886:CDS |
| GAAAGTTTGAGAATAGATAA+AGG | 0.351248 | 1.2:+8254956 | MS.gene060886:CDS |
| ACGATGAAGTTCCGCAAATT+GGG | 0.353654 | 1.2:-8255030 | None:intergenic |
| GCAGCTGAAGACCCAATTAT+CGG | 0.368315 | 1.2:-8254473 | None:intergenic |
| TCTTGGTCGATGTATTGAAT+TGG | 0.380089 | 1.2:+8254765 | MS.gene060886:CDS |
| GTTATGTCGCATAAATCTTA+TGG | 0.386596 | 1.2:+8254833 | MS.gene060886:CDS |
| AAAATAAACTGAACATTGGC+TGG | 0.400264 | 1.2:-8254602 | None:intergenic |
| TTCAATACATCGACCAAGAT+AGG | 0.418350 | 1.2:-8254761 | None:intergenic |
| ATCTTCAGATGGTATTGATT+CGG | 0.420177 | 1.2:+8254391 | MS.gene060886:CDS |
| AAAGTTTGAGAATAGATAAA+GGG | 0.425462 | 1.2:+8254957 | MS.gene060886:CDS |
| AGGAGGAGGAAAAGAGTGTT+CGG | 0.438802 | 1.2:+8255078 | MS.gene060886:CDS |
| TACTACTTTGACTCAGAAAC+GGG | 0.441375 | 1.2:+8254919 | MS.gene060886:CDS |
| ATTCGAATAAACCGATAATT+GGG | 0.445804 | 1.2:+8254462 | MS.gene060886:CDS |
| CCAAATTGCTAAGGATTGAA+AGG | 0.463067 | 1.2:-8254688 | None:intergenic |
| AAGCAGGCCATGATCGAGGC+AGG | 0.466247 | 1.2:+8254860 | MS.gene060886:CDS |
| CCAGATGGAGGAGGAGGAGG+AGG | 0.469128 | 1.2:+8255064 | MS.gene060886:CDS |
| TTCACCTGAATGCCAGGAAA+AGG | 0.471606 | 1.2:+8254793 | MS.gene060886:CDS |
| CACAATCCCTGCCTCGATCA+TGG | 0.473174 | 1.2:-8254867 | None:intergenic |
| AAACCAGATGGAGGAGGAGG+AGG | 0.478442 | 1.2:+8255061 | MS.gene060886:CDS |
| GTACTACTTTGACTCAGAAA+CGG | 0.478774 | 1.2:+8254918 | MS.gene060886:CDS |
| CCAATTGAGTTTATCAGAAA+CGG | 0.486775 | 1.2:-8254641 | None:intergenic |
| ACCAAGATAGGAAACACATC+CGG | 0.487266 | 1.2:-8254749 | None:intergenic |
| ACTGTCTCTGCCCCAATTTG+CGG | 0.487673 | 1.2:+8255019 | MS.gene060886:CDS |
| GAATGATTCACCTGAATGCC+AGG | 0.488606 | 1.2:+8254787 | MS.gene060886:CDS |
| CCGTTTCTGATAAACTCAAT+TGG | 0.492077 | 1.2:+8254641 | MS.gene060886:CDS |
| ATAAAGGGAAACAGGTTTCA+GGG | 0.497964 | 1.2:+8254972 | MS.gene060886:CDS |
| AAAGGCAATAAGTGCTGTCC+CGG | 0.518431 | 1.2:+8254730 | MS.gene060886:CDS |
| GACTATATCTTGAGGCAAGT+TGG | 0.519499 | 1.2:-8255197 | None:intergenic |
| AAGCTTATTGAATCTTCAGA+TGG | 0.525195 | 1.2:+8254380 | MS.gene060886:CDS |
| ATCAACTAGAGCAGGTAGAA+AGG | 0.527044 | 1.2:+8254712 | MS.gene060886:CDS |
| ACATCGAAAATTCTTCAGAA+TGG | 0.527971 | 1.2:-8255271 | None:intergenic |
| TTCCAAAATAAACTGAACAT+TGG | 0.543173 | 1.2:-8254606 | None:intergenic |
| GATAAACTCAATTGGCGACA+TGG | 0.543215 | 1.2:+8254649 | MS.gene060886:CDS |
| CCAAGATAGGAAACACATCC+GGG | 0.543957 | 1.2:-8254748 | None:intergenic |
| TAAATCTTATGGTGACAAGC+AGG | 0.546845 | 1.2:+8254844 | MS.gene060886:CDS |
| AATTTGGTATCAACTAGAGC+AGG | 0.548849 | 1.2:+8254704 | MS.gene060886:CDS |
| AGTGACTTAAAATGATCTGA+TGG | 0.562549 | 1.2:-8255220 | None:intergenic |
| GCAAAACCAGATGGAGGAGG+AGG | 0.565741 | 1.2:+8255058 | MS.gene060886:CDS |
| TCGTCGTGTGCAAAACCAGA+TGG | 0.569844 | 1.2:+8255049 | MS.gene060886:CDS |
| CATGAGGAAAATTGTGAAGA+GGG | 0.571586 | 1.2:+8255174 | MS.gene060886:CDS |
| AAATAAACTGAACATTGGCT+GGG | 0.578121 | 1.2:-8254601 | None:intergenic |
| ATAAACTCAATTGGCGACAT+GGG | 0.579986 | 1.2:+8254650 | MS.gene060886:CDS |
| TGAGTTTGCAAAACAACATG+AGG | 0.582736 | 1.2:+8255158 | MS.gene060886:CDS |
| TAGTTGATACCAAATTGCTA+AGG | 0.585434 | 1.2:-8254697 | None:intergenic |
| TTCAGATGGTATTGATTCGG+CGG | 0.602952 | 1.2:+8254394 | MS.gene060886:CDS |
| CGATGAAGTTCCGCAAATTG+GGG | 0.609312 | 1.2:-8255029 | None:intergenic |
| ACATGAGGAAAATTGTGAAG+AGG | 0.616000 | 1.2:+8255173 | MS.gene060886:CDS |
| GTGACTTAAAATGATCTGAT+GGG | 0.620285 | 1.2:-8255219 | None:intergenic |
| TGTGCAAAACCAGATGGAGG+AGG | 0.627739 | 1.2:+8255055 | MS.gene060886:CDS |
| TGACTCAGAAACGGGCGTCG+AGG | 0.654081 | 1.2:+8254927 | MS.gene060886:CDS |
| AGCAGGCCATGATCGAGGCA+GGG | 0.660279 | 1.2:+8254861 | MS.gene060886:CDS |
| TGACAAGCAGGCCATGATCG+AGG | 0.670822 | 1.2:+8254856 | MS.gene060886:CDS |
| TCTGATGGGACTATATCTTG+AGG | 0.670824 | 1.2:-8255205 | None:intergenic |
| TCGTGTGCAAAACCAGATGG+AGG | 0.672294 | 1.2:+8255052 | MS.gene060886:CDS |
| CAGGTTTCAGGGAACTACAA+CGG | 0.687032 | 1.2:+8254983 | MS.gene060886:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGTTTGAGAATAGATAAA+GGG | + | chr1.2:8254957-8254976 | MS.gene060886:CDS | 20.0% |
| ! | ATTCGAATAAACCGATAATT+GGG | + | chr1.2:8254462-8254481 | MS.gene060886:CDS | 25.0% |
| ! | GAAAGTTTGAGAATAGATAA+AGG | + | chr1.2:8254956-8254975 | MS.gene060886:CDS | 25.0% |
| ! | TTCCAAAATAAACTGAACAT+TGG | - | chr1.2:8254609-8254628 | None:intergenic | 25.0% |
| !! | TGAAGAGTTCTAACTAAAAA+GGG | - | chr1.2:8254503-8254522 | None:intergenic | 25.0% |
| !!! | GTTTATTTTGGAAACTGATT+TGG | + | chr1.2:8254616-8254635 | MS.gene060886:CDS | 25.0% |
| AAAATAAACTGAACATTGGC+TGG | - | chr1.2:8254605-8254624 | None:intergenic | 30.0% | |
| AAGCTTATTGAATCTTCAGA+TGG | + | chr1.2:8254380-8254399 | MS.gene060886:CDS | 30.0% | |
| AGTGACTTAAAATGATCTGA+TGG | - | chr1.2:8255223-8255242 | None:intergenic | 30.0% | |
| CCAATTGAGTTTATCAGAAA+CGG | - | chr1.2:8254644-8254663 | None:intergenic | 30.0% | |
| GATTCGAATAAACCGATAAT+TGG | + | chr1.2:8254461-8254480 | MS.gene060886:CDS | 30.0% | |
| GTGACTTAAAATGATCTGAT+GGG | - | chr1.2:8255222-8255241 | None:intergenic | 30.0% | |
| GTTATGTCGCATAAATCTTA+TGG | + | chr1.2:8254833-8254852 | MS.gene060886:CDS | 30.0% | |
| ! | AAATAAACTGAACATTGGCT+GGG | - | chr1.2:8254604-8254623 | None:intergenic | 30.0% |
| ! | AGCCAATGTTCAGTTTATTT+TGG | + | chr1.2:8254604-8254623 | MS.gene060886:CDS | 30.0% |
| ! | CTGAAGAGTTCTAACTAAAA+AGG | - | chr1.2:8254504-8254523 | None:intergenic | 30.0% |
| ! | TAGTTGATACCAAATTGCTA+AGG | - | chr1.2:8254700-8254719 | None:intergenic | 30.0% |
| ! | TTTTCTTGGATTAAGTGCTT+TGG | + | chr1.2:8254439-8254458 | MS.gene060886:CDS | 30.0% |
| !! | AAAGGTTTCGTATGTTTTGA+TGG | + | chr1.2:8254811-8254830 | MS.gene060886:CDS | 30.0% |
| !! | ATCTTCAGATGGTATTGATT+CGG | + | chr1.2:8254391-8254410 | MS.gene060886:CDS | 30.0% |
| !!! | TGTTGTTTTGCAAACTCAAT+TGG | - | chr1.2:8255156-8255175 | None:intergenic | 30.0% |
| AATTTGGTATCAACTAGAGC+AGG | + | chr1.2:8254704-8254723 | MS.gene060886:CDS | 35.0% | |
| ACATGAGGAAAATTGTGAAG+AGG | + | chr1.2:8255173-8255192 | MS.gene060886:CDS | 35.0% | |
| ATAAACTCAATTGGCGACAT+GGG | + | chr1.2:8254650-8254669 | MS.gene060886:CDS | 35.0% | |
| ATAAAGGGAAACAGGTTTCA+GGG | + | chr1.2:8254972-8254991 | MS.gene060886:CDS | 35.0% | |
| CATGAGGAAAATTGTGAAGA+GGG | + | chr1.2:8255174-8255193 | MS.gene060886:CDS | 35.0% | |
| CCAAATTGCTAAGGATTGAA+AGG | - | chr1.2:8254691-8254710 | None:intergenic | 35.0% | |
| CCGTTTCTGATAAACTCAAT+TGG | + | chr1.2:8254641-8254660 | MS.gene060886:CDS | 35.0% | |
| CCTTTCAATCCTTAGCAATT+TGG | + | chr1.2:8254688-8254707 | MS.gene060886:CDS | 35.0% | |
| GAGAATAGATAAAGGGAAAC+AGG | + | chr1.2:8254964-8254983 | MS.gene060886:CDS | 35.0% | |
| GTACTACTTTGACTCAGAAA+CGG | + | chr1.2:8254918-8254937 | MS.gene060886:CDS | 35.0% | |
| TAAATCTTATGGTGACAAGC+AGG | + | chr1.2:8254844-8254863 | MS.gene060886:CDS | 35.0% | |
| TACTACTTTGACTCAGAAAC+GGG | + | chr1.2:8254919-8254938 | MS.gene060886:CDS | 35.0% | |
| TCTTGGTCGATGTATTGAAT+TGG | + | chr1.2:8254765-8254784 | MS.gene060886:CDS | 35.0% | |
| TGAGTTTGCAAAACAACATG+AGG | + | chr1.2:8255158-8255177 | MS.gene060886:CDS | 35.0% | |
| TTCAATACATCGACCAAGAT+AGG | - | chr1.2:8254764-8254783 | None:intergenic | 35.0% | |
| ! | AAACATACGAAACCTTTTCC+TGG | - | chr1.2:8254808-8254827 | None:intergenic | 35.0% |
| ACCAAGATAGGAAACACATC+CGG | - | chr1.2:8254752-8254771 | None:intergenic | 40.0% | |
| ACGATGAAGTTCCGCAAATT+GGG | - | chr1.2:8255033-8255052 | None:intergenic | 40.0% | |
| ATCAACTAGAGCAGGTAGAA+AGG | + | chr1.2:8254712-8254731 | MS.gene060886:CDS | 40.0% | |
| GACTATATCTTGAGGCAAGT+TGG | - | chr1.2:8255200-8255219 | None:intergenic | 40.0% | |
| GATAAACTCAATTGGCGACA+TGG | + | chr1.2:8254649-8254668 | MS.gene060886:CDS | 40.0% | |
| GATAAAGGGAAACAGGTTTC+AGG | + | chr1.2:8254971-8254990 | MS.gene060886:CDS | 40.0% | |
| GGGAACTACAACGGAAATTT+AGG | + | chr1.2:8254992-8255011 | MS.gene060886:CDS | 40.0% | |
| TCTGATGGGACTATATCTTG+AGG | - | chr1.2:8255208-8255227 | None:intergenic | 40.0% | |
| ! | CTTCTTGAGTTGTCGCTTAT+TGG | + | chr1.2:8254896-8254915 | MS.gene060886:CDS | 40.0% |
| ! | GCGATTGTTGCGAATTTTCT+TGG | + | chr1.2:8254425-8254444 | MS.gene060886:CDS | 40.0% |
| !! | TTCAGATGGTATTGATTCGG+CGG | + | chr1.2:8254394-8254413 | MS.gene060886:CDS | 40.0% |
| CAGGTTTCAGGGAACTACAA+CGG | + | chr1.2:8254983-8255002 | MS.gene060886:CDS | 45.0% | |
| CCAAGATAGGAAACACATCC+GGG | - | chr1.2:8254751-8254770 | None:intergenic | 45.0% | |
| CGATGAAGTTCCGCAAATTG+GGG | - | chr1.2:8255032-8255051 | None:intergenic | 45.0% | |
| GAATGATTCACCTGAATGCC+AGG | + | chr1.2:8254787-8254806 | MS.gene060886:CDS | 45.0% | |
| GACGATGAAGTTCCGCAAAT+TGG | - | chr1.2:8255034-8255053 | None:intergenic | 45.0% | |
| GCAGCTGAAGACCCAATTAT+CGG | - | chr1.2:8254476-8254495 | None:intergenic | 45.0% | |
| TTCACCTGAATGCCAGGAAA+AGG | + | chr1.2:8254793-8254812 | MS.gene060886:CDS | 45.0% | |
| ! | AAAGGCAATAAGTGCTGTCC+CGG | + | chr1.2:8254730-8254749 | MS.gene060886:CDS | 45.0% |
| ! | GAAACCTTTTCCTGGCATTC+AGG | - | chr1.2:8254800-8254819 | None:intergenic | 45.0% |
| !! | AGGAGGAGGAAAAGAGTGTT+CGG | + | chr1.2:8255078-8255097 | MS.gene060886:CDS | 45.0% |
| ACTGTCTCTGCCCCAATTTG+CGG | + | chr1.2:8255019-8255038 | MS.gene060886:CDS | 50.0% | |
| CCCGGATGTGTTTCCTATCT+TGG | + | chr1.2:8254748-8254767 | MS.gene060886:CDS | 50.0% | |
| TCGTCGTGTGCAAAACCAGA+TGG | + | chr1.2:8255049-8255068 | MS.gene060886:CDS | 50.0% | |
| TCGTGTGCAAAACCAGATGG+AGG | + | chr1.2:8255052-8255071 | MS.gene060886:CDS | 50.0% | |
| TGTGCAAAACCAGATGGAGG+AGG | + | chr1.2:8255055-8255074 | MS.gene060886:CDS | 50.0% | |
| AAACCAGATGGAGGAGGAGG+AGG | + | chr1.2:8255061-8255080 | MS.gene060886:CDS | 55.0% | |
| CACAATCCCTGCCTCGATCA+TGG | - | chr1.2:8254870-8254889 | None:intergenic | 55.0% | |
| GCAAAACCAGATGGAGGAGG+AGG | + | chr1.2:8255058-8255077 | MS.gene060886:CDS | 55.0% | |
| TGACAAGCAGGCCATGATCG+AGG | + | chr1.2:8254856-8254875 | MS.gene060886:CDS | 55.0% | |
| AAGCAGGCCATGATCGAGGC+AGG | + | chr1.2:8254860-8254879 | MS.gene060886:CDS | 60.0% | |
| AGCAGGCCATGATCGAGGCA+GGG | + | chr1.2:8254861-8254880 | MS.gene060886:CDS | 60.0% | |
| ! | TGACTCAGAAACGGGCGTCG+AGG | + | chr1.2:8254927-8254946 | MS.gene060886:CDS | 60.0% |
| !! | GAAACGGGCGTCGAGGCTAT+TGG | + | chr1.2:8254934-8254953 | MS.gene060886:CDS | 60.0% |
| CCAGATGGAGGAGGAGGAGG+AGG | + | chr1.2:8255064-8255083 | MS.gene060886:CDS | 65.0% | |
| CCTCCTCCTCCTCCTCCATC+TGG | - | chr1.2:8255067-8255086 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 8254374 | 8255279 | 8254374 | ID=MS.gene060886 |
| chr1.2 | mRNA | 8254374 | 8255279 | 8254374 | ID=MS.gene060886.t1;Parent=MS.gene060886 |
| chr1.2 | exon | 8254374 | 8255279 | 8254374 | ID=MS.gene060886.t1.exon1;Parent=MS.gene060886.t1 |
| chr1.2 | CDS | 8254374 | 8255279 | 8254374 | ID=cds.MS.gene060886.t1;Parent=MS.gene060886.t1 |
| Gene Sequence |
| Protein sequence |
