AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060884


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060884.t1 MTR_1g014420 98.007 301 5 1 1 301 216 515 0.0 597
MS.gene060884.t1 MTR_3g106140 74.503 302 73 2 1 301 197 495 3.76e-142 410
MS.gene060884.t1 MTR_2g015870 35.433 254 132 7 52 301 215 440 2.37e-30 119
MS.gene060884.t1 MTR_8g080280 31.868 182 114 3 21 198 534 709 2.77e-22 97.4
MS.gene060884.t1 MTR_5g079550 28.253 269 156 7 15 275 116 355 8.71e-20 89.0
MS.gene060884.t1 MTR_3g085610 28.230 209 140 4 1 206 514 715 4.33e-17 82.0
MS.gene060884.t1 MTR_3g067685 27.413 259 153 8 32 285 154 382 1.93e-16 79.3
MS.gene060884.t1 MTR_4g063800 32.990 194 113 7 17 204 452 634 2.41e-16 79.7
MS.gene060884.t1 MTR_4g485520 30.978 184 117 4 17 198 472 647 2.65e-14 73.6
MS.gene060884.t1 MTR_4g485520 30.978 184 117 4 17 198 304 479 3.89e-14 72.8
MS.gene060884.t1 MTR_7g059405 29.570 186 123 3 14 199 457 634 2.46e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060884.t1 AT4G31890 66.113 301 87 5 1 301 233 518 2.77e-124 365
MS.gene060884.t1 AT4G31890 66.113 301 87 5 1 301 233 518 2.77e-124 365
MS.gene060884.t1 AT4G31890 66.113 301 87 5 1 301 233 518 2.77e-124 365
MS.gene060884.t1 AT2G25130 62.925 294 80 7 1 291 200 467 5.25e-114 337
MS.gene060884.t1 AT2G27430 32.707 266 154 5 36 301 198 438 6.47e-33 126
MS.gene060884.t1 AT1G67530 31.707 205 126 4 3 204 519 712 6.29e-21 93.6
MS.gene060884.t1 AT1G67530 31.707 205 126 4 3 204 519 712 6.29e-21 93.6
MS.gene060884.t1 AT1G67530 31.707 205 126 4 3 204 519 712 6.29e-21 93.6
MS.gene060884.t1 AT1G24330 31.707 205 131 4 20 221 531 729 1.68e-20 92.0
MS.gene060884.t1 AT1G24330 31.707 205 131 4 20 221 531 729 1.68e-20 92.0
MS.gene060884.t1 AT1G27910 33.333 183 114 4 18 198 527 703 1.76e-18 86.3
MS.gene060884.t1 AT2G28830 34.848 198 111 8 17 207 386 572 2.30e-16 79.7
MS.gene060884.t1 AT3G03440 26.894 264 156 7 21 276 169 403 1.43e-13 70.9
MS.gene060884.t1 AT3G46510 32.258 186 110 6 17 197 453 627 1.58e-13 71.2
MS.gene060884.t1 AT1G71020 31.892 185 115 4 17 198 295 471 2.15e-13 70.5
MS.gene060884.t1 AT1G71020 31.892 185 115 4 17 198 443 619 2.29e-13 70.9
MS.gene060884.t1 AT2G23140 31.016 187 120 4 13 198 449 627 8.96e-12 65.9
MS.gene060884.t1 AT2G23140 31.016 187 120 4 13 198 449 627 8.96e-12 65.9
MS.gene060884.t1 AT2G23140 31.016 187 120 4 13 198 638 816 1.39e-11 65.5
MS.gene060884.t1 AT2G23140 31.016 187 120 4 13 198 638 816 1.39e-11 65.5
MS.gene060884.t1 AT2G23140 31.016 187 120 4 13 198 641 819 1.41e-11 65.5

Find 65 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCGAATAAACCGATAAT+TGG 0.146060 1.2:+8269735 MS.gene060884:CDS
AGCCAATGTTCAGTTTATTT+TGG 0.151005 1.2:+8269878 MS.gene060884:CDS
CTTCTTGAGTTGTCGCTTAT+TGG 0.172434 1.2:+8270170 MS.gene060884:CDS
CTGAAGAGTTCTAACTAAAA+AGG 0.175660 1.2:-8269775 None:intergenic
CCTTTCAATCCTTAGCAATT+TGG 0.178700 1.2:+8269962 MS.gene060884:CDS
GACGATGAAGTTCCGCAAAT+TGG 0.211163 1.2:-8270305 None:intergenic
CCCGGATGTGTTTCCTATCT+TGG 0.249308 1.2:+8270022 MS.gene060884:CDS
GGGAACTACAACGGAAATTT+AGG 0.256409 1.2:+8270266 MS.gene060884:CDS
GATAAAGGGAAACAGGTTTC+AGG 0.257229 1.2:+8270245 MS.gene060884:CDS
GAGAATAGATAAAGGGAAAC+AGG 0.320824 1.2:+8270238 MS.gene060884:CDS
GAAACGGGCGTCGAGGCTAT+TGG 0.335825 1.2:+8270208 MS.gene060884:CDS
GAAAGTTTGAGAATAGATAA+AGG 0.351248 1.2:+8270230 MS.gene060884:CDS
ACGATGAAGTTCCGCAAATT+GGG 0.353654 1.2:-8270304 None:intergenic
GCAGCTGAAGACCCAATTAT+CGG 0.368315 1.2:-8269747 None:intergenic
TCTTGGTCGATGTATTGAAT+TGG 0.380089 1.2:+8270039 MS.gene060884:CDS
GTTATGTCGCATAAATCTTA+TGG 0.386596 1.2:+8270107 MS.gene060884:CDS
AAAATAAACTGAACATTGGC+TGG 0.400264 1.2:-8269876 None:intergenic
TTCAATACATCGACCAAGAT+AGG 0.418350 1.2:-8270035 None:intergenic
ATCTTCAGATGGTATTGATT+CGG 0.420177 1.2:+8269665 MS.gene060884:CDS
AAAGTTTGAGAATAGATAAA+GGG 0.425462 1.2:+8270231 MS.gene060884:CDS
AGGAGGAGGAAAAGAGTGTT+CGG 0.438802 1.2:+8270352 MS.gene060884:CDS
TACTACTTTGACTCAGAAAC+GGG 0.441375 1.2:+8270193 MS.gene060884:CDS
ATTCGAATAAACCGATAATT+GGG 0.445804 1.2:+8269736 MS.gene060884:CDS
CCAAATTGCTAAGGATTGAA+AGG 0.463067 1.2:-8269962 None:intergenic
AAGCAGGCCATGATCGAGGC+AGG 0.466247 1.2:+8270134 MS.gene060884:CDS
CCAGATGGAGGAGGAGGAGG+AGG 0.469128 1.2:+8270338 MS.gene060884:CDS
TTCACCTGAATGCCAGGAAA+AGG 0.471606 1.2:+8270067 MS.gene060884:CDS
CACAATCCCTGCCTCGATCA+TGG 0.473174 1.2:-8270141 None:intergenic
AAACCAGATGGAGGAGGAGG+AGG 0.478442 1.2:+8270335 MS.gene060884:CDS
GTACTACTTTGACTCAGAAA+CGG 0.478774 1.2:+8270192 MS.gene060884:CDS
CCAATTGAGTTTATCAGAAA+CGG 0.486775 1.2:-8269915 None:intergenic
ACCAAGATAGGAAACACATC+CGG 0.487266 1.2:-8270023 None:intergenic
ACTGTCTCTGCCCCAATTTG+CGG 0.487673 1.2:+8270293 MS.gene060884:CDS
GAATGATTCACCTGAATGCC+AGG 0.488606 1.2:+8270061 MS.gene060884:CDS
CCGTTTCTGATAAACTCAAT+TGG 0.492077 1.2:+8269915 MS.gene060884:CDS
ATAAAGGGAAACAGGTTTCA+GGG 0.497964 1.2:+8270246 MS.gene060884:CDS
AAAGGCAATAAGTGCTGTCC+CGG 0.518431 1.2:+8270004 MS.gene060884:CDS
GACTATATCTTGAGGCAAGT+TGG 0.519499 1.2:-8270471 None:intergenic
AAGCTTATTGAATCTTCAGA+TGG 0.525195 1.2:+8269654 MS.gene060884:CDS
ATCAACTAGAGCAGGTAGAA+AGG 0.527044 1.2:+8269986 MS.gene060884:CDS
ACATCGAAAATTCTTCAGAA+TGG 0.527971 1.2:-8270545 None:intergenic
TTCCAAAATAAACTGAACAT+TGG 0.543173 1.2:-8269880 None:intergenic
GATAAACTCAATTGGCGACA+TGG 0.543215 1.2:+8269923 MS.gene060884:CDS
CCAAGATAGGAAACACATCC+GGG 0.543957 1.2:-8270022 None:intergenic
TAAATCTTATGGTGACAAGC+AGG 0.546845 1.2:+8270118 MS.gene060884:CDS
AATTTGGTATCAACTAGAGC+AGG 0.548849 1.2:+8269978 MS.gene060884:CDS
AGTGACTTAAAATGATCTGA+TGG 0.562549 1.2:-8270494 None:intergenic
GCAAAACCAGATGGAGGAGG+AGG 0.565741 1.2:+8270332 MS.gene060884:CDS
TCGTCGTGTGCAAAACCAGA+TGG 0.569844 1.2:+8270323 MS.gene060884:CDS
CATGAGGAAAATTGTGAAGA+GGG 0.571586 1.2:+8270448 MS.gene060884:CDS
AAATAAACTGAACATTGGCT+GGG 0.578121 1.2:-8269875 None:intergenic
ATAAACTCAATTGGCGACAT+GGG 0.579986 1.2:+8269924 MS.gene060884:CDS
TGAGTTTGCAAAACAACATG+AGG 0.582736 1.2:+8270432 MS.gene060884:CDS
TAGTTGATACCAAATTGCTA+AGG 0.585434 1.2:-8269971 None:intergenic
TTCAGATGGTATTGATTCGG+CGG 0.602952 1.2:+8269668 MS.gene060884:CDS
CGATGAAGTTCCGCAAATTG+GGG 0.609312 1.2:-8270303 None:intergenic
ACATGAGGAAAATTGTGAAG+AGG 0.616000 1.2:+8270447 MS.gene060884:CDS
GTGACTTAAAATGATCTGAT+GGG 0.620285 1.2:-8270493 None:intergenic
TGTGCAAAACCAGATGGAGG+AGG 0.627739 1.2:+8270329 MS.gene060884:CDS
TGACTCAGAAACGGGCGTCG+AGG 0.654081 1.2:+8270201 MS.gene060884:CDS
AGCAGGCCATGATCGAGGCA+GGG 0.660279 1.2:+8270135 MS.gene060884:CDS
TGACAAGCAGGCCATGATCG+AGG 0.670822 1.2:+8270130 MS.gene060884:CDS
TCTGATGGGACTATATCTTG+AGG 0.670824 1.2:-8270479 None:intergenic
TCGTGTGCAAAACCAGATGG+AGG 0.672294 1.2:+8270326 MS.gene060884:CDS
CAGGTTTCAGGGAACTACAA+CGG 0.687032 1.2:+8270257 MS.gene060884:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTTTGAGAATAGATAAA+GGG + chr1.2:8270231-8270250 MS.gene060884:CDS 20.0%
! ATTCGAATAAACCGATAATT+GGG + chr1.2:8269736-8269755 MS.gene060884:CDS 25.0%
! GAAAGTTTGAGAATAGATAA+AGG + chr1.2:8270230-8270249 MS.gene060884:CDS 25.0%
! TTCCAAAATAAACTGAACAT+TGG - chr1.2:8269883-8269902 None:intergenic 25.0%
!! TGAAGAGTTCTAACTAAAAA+GGG - chr1.2:8269777-8269796 None:intergenic 25.0%
!!! GTTTATTTTGGAAACTGATT+TGG + chr1.2:8269890-8269909 MS.gene060884:CDS 25.0%
AAAATAAACTGAACATTGGC+TGG - chr1.2:8269879-8269898 None:intergenic 30.0%
AAGCTTATTGAATCTTCAGA+TGG + chr1.2:8269654-8269673 MS.gene060884:CDS 30.0%
AGTGACTTAAAATGATCTGA+TGG - chr1.2:8270497-8270516 None:intergenic 30.0%
CCAATTGAGTTTATCAGAAA+CGG - chr1.2:8269918-8269937 None:intergenic 30.0%
GATTCGAATAAACCGATAAT+TGG + chr1.2:8269735-8269754 MS.gene060884:CDS 30.0%
GTGACTTAAAATGATCTGAT+GGG - chr1.2:8270496-8270515 None:intergenic 30.0%
GTTATGTCGCATAAATCTTA+TGG + chr1.2:8270107-8270126 MS.gene060884:CDS 30.0%
! AAATAAACTGAACATTGGCT+GGG - chr1.2:8269878-8269897 None:intergenic 30.0%
! AGCCAATGTTCAGTTTATTT+TGG + chr1.2:8269878-8269897 MS.gene060884:CDS 30.0%
! CTGAAGAGTTCTAACTAAAA+AGG - chr1.2:8269778-8269797 None:intergenic 30.0%
! TAGTTGATACCAAATTGCTA+AGG - chr1.2:8269974-8269993 None:intergenic 30.0%
! TTTTCTTGGATTAAGTGCTT+TGG + chr1.2:8269713-8269732 MS.gene060884:CDS 30.0%
!! AAAGGTTTCGTATGTTTTGA+TGG + chr1.2:8270085-8270104 MS.gene060884:CDS 30.0%
!! ATCTTCAGATGGTATTGATT+CGG + chr1.2:8269665-8269684 MS.gene060884:CDS 30.0%
!!! TGTTGTTTTGCAAACTCAAT+TGG - chr1.2:8270430-8270449 None:intergenic 30.0%
AATTTGGTATCAACTAGAGC+AGG + chr1.2:8269978-8269997 MS.gene060884:CDS 35.0%
ACATGAGGAAAATTGTGAAG+AGG + chr1.2:8270447-8270466 MS.gene060884:CDS 35.0%
ATAAACTCAATTGGCGACAT+GGG + chr1.2:8269924-8269943 MS.gene060884:CDS 35.0%
ATAAAGGGAAACAGGTTTCA+GGG + chr1.2:8270246-8270265 MS.gene060884:CDS 35.0%
CATGAGGAAAATTGTGAAGA+GGG + chr1.2:8270448-8270467 MS.gene060884:CDS 35.0%
CCAAATTGCTAAGGATTGAA+AGG - chr1.2:8269965-8269984 None:intergenic 35.0%
CCGTTTCTGATAAACTCAAT+TGG + chr1.2:8269915-8269934 MS.gene060884:CDS 35.0%
CCTTTCAATCCTTAGCAATT+TGG + chr1.2:8269962-8269981 MS.gene060884:CDS 35.0%
GAGAATAGATAAAGGGAAAC+AGG + chr1.2:8270238-8270257 MS.gene060884:CDS 35.0%
GTACTACTTTGACTCAGAAA+CGG + chr1.2:8270192-8270211 MS.gene060884:CDS 35.0%
TAAATCTTATGGTGACAAGC+AGG + chr1.2:8270118-8270137 MS.gene060884:CDS 35.0%
TACTACTTTGACTCAGAAAC+GGG + chr1.2:8270193-8270212 MS.gene060884:CDS 35.0%
TCTTGGTCGATGTATTGAAT+TGG + chr1.2:8270039-8270058 MS.gene060884:CDS 35.0%
TGAGTTTGCAAAACAACATG+AGG + chr1.2:8270432-8270451 MS.gene060884:CDS 35.0%
TTCAATACATCGACCAAGAT+AGG - chr1.2:8270038-8270057 None:intergenic 35.0%
! AAACATACGAAACCTTTTCC+TGG - chr1.2:8270082-8270101 None:intergenic 35.0%
ACCAAGATAGGAAACACATC+CGG - chr1.2:8270026-8270045 None:intergenic 40.0%
ACGATGAAGTTCCGCAAATT+GGG - chr1.2:8270307-8270326 None:intergenic 40.0%
ATCAACTAGAGCAGGTAGAA+AGG + chr1.2:8269986-8270005 MS.gene060884:CDS 40.0%
GACTATATCTTGAGGCAAGT+TGG - chr1.2:8270474-8270493 None:intergenic 40.0%
GATAAACTCAATTGGCGACA+TGG + chr1.2:8269923-8269942 MS.gene060884:CDS 40.0%
GATAAAGGGAAACAGGTTTC+AGG + chr1.2:8270245-8270264 MS.gene060884:CDS 40.0%
GGGAACTACAACGGAAATTT+AGG + chr1.2:8270266-8270285 MS.gene060884:CDS 40.0%
TCTGATGGGACTATATCTTG+AGG - chr1.2:8270482-8270501 None:intergenic 40.0%
! CTTCTTGAGTTGTCGCTTAT+TGG + chr1.2:8270170-8270189 MS.gene060884:CDS 40.0%
! GCGATTGTTGCGAATTTTCT+TGG + chr1.2:8269699-8269718 MS.gene060884:CDS 40.0%
!! TTCAGATGGTATTGATTCGG+CGG + chr1.2:8269668-8269687 MS.gene060884:CDS 40.0%
CAGGTTTCAGGGAACTACAA+CGG + chr1.2:8270257-8270276 MS.gene060884:CDS 45.0%
CCAAGATAGGAAACACATCC+GGG - chr1.2:8270025-8270044 None:intergenic 45.0%
CGATGAAGTTCCGCAAATTG+GGG - chr1.2:8270306-8270325 None:intergenic 45.0%
GAATGATTCACCTGAATGCC+AGG + chr1.2:8270061-8270080 MS.gene060884:CDS 45.0%
GACGATGAAGTTCCGCAAAT+TGG - chr1.2:8270308-8270327 None:intergenic 45.0%
GCAGCTGAAGACCCAATTAT+CGG - chr1.2:8269750-8269769 None:intergenic 45.0%
TTCACCTGAATGCCAGGAAA+AGG + chr1.2:8270067-8270086 MS.gene060884:CDS 45.0%
! AAAGGCAATAAGTGCTGTCC+CGG + chr1.2:8270004-8270023 MS.gene060884:CDS 45.0%
! GAAACCTTTTCCTGGCATTC+AGG - chr1.2:8270074-8270093 None:intergenic 45.0%
!! AGGAGGAGGAAAAGAGTGTT+CGG + chr1.2:8270352-8270371 MS.gene060884:CDS 45.0%
ACTGTCTCTGCCCCAATTTG+CGG + chr1.2:8270293-8270312 MS.gene060884:CDS 50.0%
CCCGGATGTGTTTCCTATCT+TGG + chr1.2:8270022-8270041 MS.gene060884:CDS 50.0%
TCGTCGTGTGCAAAACCAGA+TGG + chr1.2:8270323-8270342 MS.gene060884:CDS 50.0%
TCGTGTGCAAAACCAGATGG+AGG + chr1.2:8270326-8270345 MS.gene060884:CDS 50.0%
TGTGCAAAACCAGATGGAGG+AGG + chr1.2:8270329-8270348 MS.gene060884:CDS 50.0%
AAACCAGATGGAGGAGGAGG+AGG + chr1.2:8270335-8270354 MS.gene060884:CDS 55.0%
CACAATCCCTGCCTCGATCA+TGG - chr1.2:8270144-8270163 None:intergenic 55.0%
GCAAAACCAGATGGAGGAGG+AGG + chr1.2:8270332-8270351 MS.gene060884:CDS 55.0%
TGACAAGCAGGCCATGATCG+AGG + chr1.2:8270130-8270149 MS.gene060884:CDS 55.0%
AAGCAGGCCATGATCGAGGC+AGG + chr1.2:8270134-8270153 MS.gene060884:CDS 60.0%
AGCAGGCCATGATCGAGGCA+GGG + chr1.2:8270135-8270154 MS.gene060884:CDS 60.0%
! TGACTCAGAAACGGGCGTCG+AGG + chr1.2:8270201-8270220 MS.gene060884:CDS 60.0%
!! GAAACGGGCGTCGAGGCTAT+TGG + chr1.2:8270208-8270227 MS.gene060884:CDS 60.0%
CCAGATGGAGGAGGAGGAGG+AGG + chr1.2:8270338-8270357 MS.gene060884:CDS 65.0%
CCTCCTCCTCCTCCTCCATC+TGG - chr1.2:8270341-8270360 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 8269648 8270553 8269648 ID=MS.gene060884
chr1.2 mRNA 8269648 8270553 8269648 ID=MS.gene060884.t1;Parent=MS.gene060884
chr1.2 exon 8269648 8270553 8269648 ID=MS.gene060884.t1.exon1;Parent=MS.gene060884.t1
chr1.2 CDS 8269648 8270553 8269648 ID=cds.MS.gene060884.t1;Parent=MS.gene060884.t1
Gene Sequence

>MS.gene060884

ATGCTTAAGCTTATTGAATCTTCAGATGGTATTGATTCGGCGGTTTCTGAAGCGATTGTTGCGAATTTTCTTGGATTAAGTGCTTTGGATTCGAATAAACCGATAATTGGGTCTTCAGCTGCAATACCCTTTTTAGTTAGAACTCTTCAGAATAAGAATATAGATAAGAAAAGTAGCAATCAAGTTAAGCAAGATGCTCTTAGAGCACTTTACAATCTTTCAATCTTCCCAGCCAATGTTCAGTTTATTTTGGAAACTGATTTGGTACCGTTTCTGATAAACTCAATTGGCGACATGGGAGTAACTGAAAGAAACCTTTCAATCCTTAGCAATTTGGTATCAACTAGAGCAGGTAGAAAGGCAATAAGTGCTGTCCCGGATGTGTTTCCTATCTTGGTCGATGTATTGAATTGGAATGATTCACCTGAATGCCAGGAAAAGGTTTCGTATGTTTTGATGGTTATGTCGCATAAATCTTATGGTGACAAGCAGGCCATGATCGAGGCAGGGATTGTGTCGTCGCTTCTTGAGTTGTCGCTTATTGGTACTACTTTGACTCAGAAACGGGCGTCGAGGCTATTGGAAAGTTTGAGAATAGATAAAGGGAAACAGGTTTCAGGGAACTACAACGGAAATTTAGGTGCAACTGTCTCTGCCCCAATTTGCGGAACTTCATCGTCGTGTGCAAAACCAGATGGAGGAGGAGGAGGAGGAAAAGAGTGTTCGGAAGAAGATGAAGATATGATGAGCGAAGAGAAGAAAGCAGTGAAGCAATTAGTCCAATTGAGTTTGCAAAACAACATGAGGAAAATTGTGAAGAGGGCCAACTTGCCTCAAGATATAGTCCCATCAGATCATTTTAAGTCACTCACTTCAAGTTCAACTTCAAAGAGTTTACCATTCTGA

Protein sequence

>MS.gene060884.t1

MLKLIESSDGIDSAVSEAIVANFLGLSALDSNKPIIGSSAAIPFLVRTLQNKNIDKKSSNQVKQDALRALYNLSIFPANVQFILETDLVPFLINSIGDMGVTERNLSILSNLVSTRAGRKAISAVPDVFPILVDVLNWNDSPECQEKVSYVLMVMSHKSYGDKQAMIEAGIVSSLLELSLIGTTLTQKRASRLLESLRIDKGKQVSGNYNGNLGATVSAPICGTSSSCAKPDGGGGGGKECSEEDEDMMSEEKKAVKQLVQLSLQNNMRKIVKRANLPQDIVPSDHFKSLTSSSTSKSLPF