Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30740.t1 | KEH38081.1 | 96.8 | 308 | 8 | 1 | 1 | 308 | 1 | 306 | 2.40E-158 | 568.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30740.t1 | Q94A09 | 65.7 | 309 | 71 | 3 | 35 | 308 | 64 | 372 | 1.9e-103 | 377.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30740.t1 | A0A072V8X2 | 96.8 | 308 | 8 | 1 | 1 | 308 | 1 | 306 | 1.7e-158 | 568.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050945 | MS.gene30740 | 0.826938 | 2.06E-54 | -1.69E-46 |
| MS.gene051234 | MS.gene30740 | 0.818928 | 1.52E-52 | -1.69E-46 |
| MS.gene054178 | MS.gene30740 | 0.80941 | 1.92E-50 | -1.69E-46 |
| MS.gene059146 | MS.gene30740 | 0.825986 | 3.48E-54 | -1.69E-46 |
| MS.gene061100 | MS.gene30740 | 0.824514 | 7.75E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30740.t1 | MTR_2g460880 | 95.130 | 308 | 13 | 1 | 1 | 308 | 1 | 306 | 0.0 | 580 |
| MS.gene30740.t1 | MTR_2g460880 | 95.130 | 308 | 13 | 1 | 1 | 308 | 1 | 306 | 0.0 | 580 |
| MS.gene30740.t1 | MTR_2g460880 | 95.114 | 307 | 13 | 1 | 1 | 307 | 1 | 305 | 0.0 | 579 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene30740.t1 | AT1G36310 | 63.663 | 344 | 89 | 5 | 1 | 308 | 29 | 372 | 6.34e-132 | 381 |
| MS.gene30740.t1 | AT1G36310 | 63.663 | 344 | 89 | 5 | 1 | 308 | 29 | 372 | 6.34e-132 | 381 |
Find 64 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTGGGAATGGAAAGTATTT+AGG | 0.153553 | 3.1:-60252957 | MS.gene30740:CDS |
| ATTGGTTAGGGTTGTTAAAA+AGG | 0.210227 | 3.1:-60252736 | MS.gene30740:CDS |
| GTTCGACCCGTTATGCAAAA+TGG | 0.244137 | 3.1:-60253040 | MS.gene30740:CDS |
| TTGCGATGAGTCCGAAGTTT+TGG | 0.246310 | 3.1:-60252511 | MS.gene30740:CDS |
| CTTCCATATAGAACTGGTTT+TGG | 0.281591 | 3.1:-60252831 | MS.gene30740:CDS |
| GTGGGAATGGAAAGTATTTA+GGG | 0.290355 | 3.1:-60252956 | MS.gene30740:CDS |
| AAACCACTCTCCTCACTTTC+CGG | 0.306191 | 3.1:+60252555 | None:intergenic |
| TCTGCTCATGCTTTGGCTAC+TGG | 0.313548 | 3.1:-60252396 | MS.gene30740:CDS |
| GCGAAAAGCAATTGAAGAAT+TGG | 0.336435 | 3.1:-60252754 | MS.gene30740:CDS |
| AAGCAATTGAAGAATTGGTT+AGG | 0.348953 | 3.1:-60252749 | MS.gene30740:CDS |
| TGCTACTAAAGATGATATAA+AGG | 0.351714 | 3.1:-60252370 | MS.gene30740:CDS |
| TCACCAAAACCAGTTCTATA+TGG | 0.359161 | 3.1:+60252828 | None:intergenic |
| ATTAAGATTTGTTTAGATAG+AGG | 0.380488 | 3.1:-60252885 | MS.gene30740:CDS |
| TGTAGAAATTCAGCAACTTT+CGG | 0.385442 | 3.1:+60253017 | None:intergenic |
| GAAAGTGAGGAGAGTGGTTT+GGG | 0.396880 | 3.1:-60252552 | MS.gene30740:CDS |
| GTTGTCGTTGGAAAGTATTC+CGG | 0.398684 | 3.1:-60252574 | MS.gene30740:CDS |
| CGAGTAGCACGTGGACTTCC+CGG | 0.400143 | 3.1:+60252609 | None:intergenic |
| GTTAATCTTCCATATAGAAC+TGG | 0.412119 | 3.1:-60252837 | MS.gene30740:CDS |
| TGGTGCTTCTGCTCATGCTT+TGG | 0.413666 | 3.1:-60252403 | MS.gene30740:CDS |
| CGGGAAGTCCACGTGCTACT+CGG | 0.424013 | 3.1:-60252608 | MS.gene30740:CDS |
| GGTGTCTTCTTCGTTGTCGT+TGG | 0.424077 | 3.1:-60252586 | MS.gene30740:CDS |
| GGAAAGTGAGGAGAGTGGTT+TGG | 0.441010 | 3.1:-60252553 | MS.gene30740:CDS |
| CTGACTTCAGCGCGATGATA+AGG | 0.451316 | 3.1:+60252426 | None:intergenic |
| TTTCTACAAAATTTGCATCC+AGG | 0.461897 | 3.1:-60253002 | MS.gene30740:CDS |
| ATGAGGCGTTGGTTAAGAAA+TGG | 0.464016 | 3.1:-60252671 | MS.gene30740:CDS |
| ATTTGAGTACTGAGAATAGA+AGG | 0.466020 | 3.1:-60252776 | MS.gene30740:CDS |
| GCTACTAAAGATGATATAAA+GGG | 0.466972 | 3.1:-60252369 | MS.gene30740:CDS |
| AGCAATTGAAGAATTGGTTA+GGG | 0.475800 | 3.1:-60252748 | MS.gene30740:CDS |
| TGTTGAAGAATGGCAGGGAC+CGG | 0.479768 | 3.1:-60252628 | MS.gene30740:CDS |
| CATTCCCACATCCTGCATCC+AGG | 0.488324 | 3.1:+60252970 | None:intergenic |
| AAACAAATCTTAATCAATGA+AGG | 0.489573 | 3.1:+60252894 | None:intergenic |
| AAAGAATGTGAAGAATCAAC+AGG | 0.490219 | 3.1:-60252472 | MS.gene30740:CDS |
| TGATGAATTGTGTGTGATGT+AGG | 0.502313 | 3.1:+60253184 | None:intergenic |
| GCATCCAGGATCAATGATCC+TGG | 0.507454 | 3.1:+60252984 | None:intergenic |
| GAAGTATGTTGAAGAATGGC+AGG | 0.508849 | 3.1:-60252634 | MS.gene30740:CDS |
| GCATCCAGGATCATTGATCC+TGG | 0.514150 | 3.1:-60252988 | MS.gene30740:CDS |
| GGATCATTGATCCTGGATGC+AGG | 0.515522 | 3.1:-60252981 | MS.gene30740:CDS |
| GGGAAGTCCACGTGCTACTC+GGG | 0.516924 | 3.1:-60252607 | MS.gene30740:CDS |
| GGAGAGTGGTTTGGGTGGTG+AGG | 0.519039 | 3.1:-60252544 | MS.gene30740:CDS |
| TTAGCGAAGGCGAGCTTGAA+AGG | 0.519347 | 3.1:-60252314 | MS.gene30740:CDS |
| GGCTGTTGAACAAGAGGATG+AGG | 0.522357 | 3.1:-60252688 | MS.gene30740:CDS |
| TTGATCCTGGATGCAGGATG+TGG | 0.522669 | 3.1:-60252975 | MS.gene30740:CDS |
| CTTCAAATCTCCCAAAACTT+CGG | 0.542427 | 3.1:+60252500 | None:intergenic |
| AACGGGTCGAACTAAAATGT+GGG | 0.551768 | 3.1:+60253051 | None:intergenic |
| CTGGATGCAGGATGTGGGAA+TGG | 0.555129 | 3.1:-60252969 | MS.gene30740:CDS |
| CTTATAGATGATACTGCAGC+TGG | 0.559553 | 3.1:+60253134 | None:intergenic |
| TCAACATACTTCTCAGCAAG+CGG | 0.560715 | 3.1:+60252645 | None:intergenic |
| TGAACAAGAGGATGAGGCGT+TGG | 0.562919 | 3.1:-60252682 | MS.gene30740:CDS |
| GTTGAAGAATGGCAGGGACC+GGG | 0.566489 | 3.1:-60252627 | MS.gene30740:CDS |
| CTGAGAAGTATGTTGAAGAA+TGG | 0.575749 | 3.1:-60252638 | MS.gene30740:CDS |
| GATGAATTGTGTGTGATGTA+GGG | 0.581198 | 3.1:+60253185 | None:intergenic |
| TAACGGGTCGAACTAAAATG+TGG | 0.590195 | 3.1:+60253050 | None:intergenic |
| AGTGAGGAGAGTGGTTTGGG+TGG | 0.597452 | 3.1:-60252549 | MS.gene30740:CDS |
| CGATGATAAGGTAAATGCCA+AGG | 0.604941 | 3.1:+60252438 | None:intergenic |
| GGAAAGTATTCCGGAAAGTG+AGG | 0.620911 | 3.1:-60252565 | MS.gene30740:CDS |
| TGTTTGGGCTGTTGAACAAG+AGG | 0.638871 | 3.1:-60252694 | MS.gene30740:CDS |
| TATCATCGCGCTGAAGTCAG+TGG | 0.641644 | 3.1:-60252423 | MS.gene30740:CDS |
| TGATCCTGGATGCAGGATGT+GGG | 0.666318 | 3.1:-60252974 | MS.gene30740:CDS |
| AAGTATGTTGAAGAATGGCA+GGG | 0.670774 | 3.1:-60252633 | MS.gene30740:CDS |
| GAAGACACCCGAGTAGCACG+TGG | 0.670935 | 3.1:+60252600 | None:intergenic |
| ATTCCGGAAAGTGAGGAGAG+TGG | 0.676753 | 3.1:-60252558 | MS.gene30740:CDS |
| ACGGGTCGAACTAAAATGTG+GGG | 0.683186 | 3.1:+60253052 | None:intergenic |
| GGGCAATTGCATCATAAACA+TGG | 0.702606 | 3.1:+60253072 | None:intergenic |
| ATAGATGATACTGCAGCTGG+TGG | 0.725906 | 3.1:+60253137 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAAGATTGTTTTTTTAT+AGG | - | chr3.1:60252596-60252615 | MS.gene30740:CDS | 10.0% |
| !! | AAACAAATCTTAATCAATGA+AGG | + | chr3.1:60252635-60252654 | None:intergenic | 20.0% |
| !!! | ACATACTTTTTTTCAATTTC+AGG | + | chr3.1:60252431-60252450 | None:intergenic | 20.0% |
| !!! | ATACTTTTTTTCAATTTCAG+GGG | + | chr3.1:60252429-60252448 | None:intergenic | 20.0% |
| !!! | ATTAAGATTTGTTTAGATAG+AGG | - | chr3.1:60252641-60252660 | MS.gene30740:CDS | 20.0% |
| !!! | CATACTTTTTTTCAATTTCA+GGG | + | chr3.1:60252430-60252449 | None:intergenic | 20.0% |
| ! | GCTACTAAAGATGATATAAA+GGG | - | chr3.1:60253157-60253176 | MS.gene30740:CDS | 25.0% |
| ! | TGCTACTAAAGATGATATAA+AGG | - | chr3.1:60253156-60253175 | MS.gene30740:CDS | 25.0% |
| !!! | TATTATCATGTTTTTAGCGA+AGG | - | chr3.1:60253199-60253218 | MS.gene30740:CDS | 25.0% |
| AAAGAATGTGAAGAATCAAC+AGG | - | chr3.1:60253054-60253073 | MS.gene30740:CDS | 30.0% | |
| AAGCAATTGAAGAATTGGTT+AGG | - | chr3.1:60252777-60252796 | MS.gene30740:CDS | 30.0% | |
| AGCAATTGAAGAATTGGTTA+GGG | - | chr3.1:60252778-60252797 | MS.gene30740:CDS | 30.0% | |
| ATTGGTTAGGGTTGTTAAAA+AGG | - | chr3.1:60252790-60252809 | MS.gene30740:CDS | 30.0% | |
| GTTAATCTTCCATATAGAAC+TGG | - | chr3.1:60252689-60252708 | MS.gene30740:CDS | 30.0% | |
| TGTAGAAATTCAGCAACTTT+CGG | + | chr3.1:60252512-60252531 | None:intergenic | 30.0% | |
| TTGGTTAGGGTTGTTAAAAA+GGG | - | chr3.1:60252791-60252810 | MS.gene30740:CDS | 30.0% | |
| TTTCTACAAAATTTGCATCC+AGG | - | chr3.1:60252524-60252543 | MS.gene30740:CDS | 30.0% | |
| !! | ATTTGAGTACTGAGAATAGA+AGG | - | chr3.1:60252750-60252769 | MS.gene30740:CDS | 30.0% |
| !!! | GCTTGTTTTGATTACTGTTT+GGG | - | chr3.1:60252817-60252836 | MS.gene30740:CDS | 30.0% |
| AAGTATGTTGAAGAATGGCA+GGG | - | chr3.1:60252893-60252912 | MS.gene30740:CDS | 35.0% | |
| CTTCAAATCTCCCAAAACTT+CGG | + | chr3.1:60253029-60253048 | None:intergenic | 35.0% | |
| GATGAATTGTGTGTGATGTA+GGG | + | chr3.1:60252344-60252363 | None:intergenic | 35.0% | |
| GCGAAAAGCAATTGAAGAAT+TGG | - | chr3.1:60252772-60252791 | MS.gene30740:CDS | 35.0% | |
| TCACCAAAACCAGTTCTATA+TGG | + | chr3.1:60252701-60252720 | None:intergenic | 35.0% | |
| TGATGAATTGTGTGTGATGT+AGG | + | chr3.1:60252345-60252364 | None:intergenic | 35.0% | |
| TTAGGGTTGTTAAAAAGGGT+GGG | - | chr3.1:60252795-60252814 | MS.gene30740:CDS | 35.0% | |
| ! | CTGAGAAGTATGTTGAAGAA+TGG | - | chr3.1:60252888-60252907 | MS.gene30740:CDS | 35.0% |
| !! | CTTCCATATAGAACTGGTTT+TGG | - | chr3.1:60252695-60252714 | MS.gene30740:CDS | 35.0% |
| !! | GTGGGAATGGAAAGTATTTA+GGG | - | chr3.1:60252570-60252589 | MS.gene30740:CDS | 35.0% |
| !! | TGTGGGAATGGAAAGTATTT+AGG | - | chr3.1:60252569-60252588 | MS.gene30740:CDS | 35.0% |
| !!! | AACAGGAGTATTTTGTTCCT+TGG | - | chr3.1:60253071-60253090 | MS.gene30740:CDS | 35.0% |
| !!! | CTTTTTTTCAATTTCAGGGG+TGG | + | chr3.1:60252426-60252445 | None:intergenic | 35.0% |
| !!! | GGCTTGTTTTGATTACTGTT+TGG | - | chr3.1:60252816-60252835 | MS.gene30740:CDS | 35.0% |
| AACGGGTCGAACTAAAATGT+GGG | + | chr3.1:60252478-60252497 | None:intergenic | 40.0% | |
| CGATGATAAGGTAAATGCCA+AGG | + | chr3.1:60253091-60253110 | None:intergenic | 40.0% | |
| CTTATAGATGATACTGCAGC+TGG | + | chr3.1:60252395-60252414 | None:intergenic | 40.0% | |
| GAAGTATGTTGAAGAATGGC+AGG | - | chr3.1:60252892-60252911 | MS.gene30740:CDS | 40.0% | |
| GGGCAATTGCATCATAAACA+TGG | + | chr3.1:60252457-60252476 | None:intergenic | 40.0% | |
| GTTAGGGTTGTTAAAAAGGG+TGG | - | chr3.1:60252794-60252813 | MS.gene30740:CDS | 40.0% | |
| TCAACATACTTCTCAGCAAG+CGG | + | chr3.1:60252884-60252903 | None:intergenic | 40.0% | |
| ! | TAACGGGTCGAACTAAAATG+TGG | + | chr3.1:60252479-60252498 | None:intergenic | 40.0% |
| !! | ATGAGGCGTTGGTTAAGAAA+TGG | - | chr3.1:60252855-60252874 | MS.gene30740:CDS | 40.0% |
| !! | CTTTCGGCCATTTTGCATAA+CGG | + | chr3.1:60252496-60252515 | None:intergenic | 40.0% |
| !! | GTTGTCGTTGGAAAGTATTC+CGG | - | chr3.1:60252952-60252971 | MS.gene30740:CDS | 40.0% |
| !! | TTTCGGCCATTTTGCATAAC+GGG | + | chr3.1:60252495-60252514 | None:intergenic | 40.0% |
| !!! | CGAAGTTTTGGGAGATTTGA+AGG | - | chr3.1:60253027-60253046 | MS.gene30740:CDS | 40.0% |
| AAACCACTCTCCTCACTTTC+CGG | + | chr3.1:60252974-60252993 | None:intergenic | 45.0% | |
| ACGGGTCGAACTAAAATGTG+GGG | + | chr3.1:60252477-60252496 | None:intergenic | 45.0% | |
| ATAGATGATACTGCAGCTGG+TGG | + | chr3.1:60252392-60252411 | None:intergenic | 45.0% | |
| GAAAGTGAGGAGAGTGGTTT+GGG | - | chr3.1:60252974-60252993 | MS.gene30740:CDS | 45.0% | |
| GTTCGACCCGTTATGCAAAA+TGG | - | chr3.1:60252486-60252505 | MS.gene30740:CDS | 45.0% | |
| TGTTTGGGCTGTTGAACAAG+AGG | - | chr3.1:60252832-60252851 | MS.gene30740:CDS | 45.0% | |
| ! | TGCGATGAGTCCGAAGTTTT+GGG | - | chr3.1:60253016-60253035 | MS.gene30740:CDS | 45.0% |
| ! | TTGCGATGAGTCCGAAGTTT+TGG | - | chr3.1:60253015-60253034 | MS.gene30740:CDS | 45.0% |
| !! | GGAAAGTATTCCGGAAAGTG+AGG | - | chr3.1:60252961-60252980 | MS.gene30740:CDS | 45.0% |
| ATTCCGGAAAGTGAGGAGAG+TGG | - | chr3.1:60252968-60252987 | MS.gene30740:CDS | 50.0% | |
| CTGACTTCAGCGCGATGATA+AGG | + | chr3.1:60253103-60253122 | None:intergenic | 50.0% | |
| GCATCCAGGATCAATGATCC+TGG | + | chr3.1:60252545-60252564 | None:intergenic | 50.0% | |
| GCATCCAGGATCATTGATCC+TGG | - | chr3.1:60252538-60252557 | MS.gene30740:CDS | 50.0% | |
| GGAAAGTGAGGAGAGTGGTT+TGG | - | chr3.1:60252973-60252992 | MS.gene30740:CDS | 50.0% | |
| GGATCATTGATCCTGGATGC+AGG | - | chr3.1:60252545-60252564 | MS.gene30740:CDS | 50.0% | |
| GGCTGTTGAACAAGAGGATG+AGG | - | chr3.1:60252838-60252857 | MS.gene30740:CDS | 50.0% | |
| GGTGTCTTCTTCGTTGTCGT+TGG | - | chr3.1:60252940-60252959 | MS.gene30740:CDS | 50.0% | |
| TATCATCGCGCTGAAGTCAG+TGG | - | chr3.1:60253103-60253122 | MS.gene30740:CDS | 50.0% | |
| TCTGCTCATGCTTTGGCTAC+TGG | - | chr3.1:60253130-60253149 | MS.gene30740:CDS | 50.0% | |
| TGAACAAGAGGATGAGGCGT+TGG | - | chr3.1:60252844-60252863 | MS.gene30740:CDS | 50.0% | |
| TGATCCTGGATGCAGGATGT+GGG | - | chr3.1:60252552-60252571 | MS.gene30740:CDS | 50.0% | |
| TGGTGCTTCTGCTCATGCTT+TGG | - | chr3.1:60253123-60253142 | MS.gene30740:CDS | 50.0% | |
| TGTTGAAGAATGGCAGGGAC+CGG | - | chr3.1:60252898-60252917 | MS.gene30740:CDS | 50.0% | |
| TTAGCGAAGGCGAGCTTGAA+AGG | - | chr3.1:60253212-60253231 | MS.gene30740:CDS | 50.0% | |
| TTGATCCTGGATGCAGGATG+TGG | - | chr3.1:60252551-60252570 | MS.gene30740:CDS | 50.0% | |
| CATTCCCACATCCTGCATCC+AGG | + | chr3.1:60252559-60252578 | None:intergenic | 55.0% | |
| CTGGATGCAGGATGTGGGAA+TGG | - | chr3.1:60252557-60252576 | MS.gene30740:CDS | 55.0% | |
| GTTGAAGAATGGCAGGGACC+GGG | - | chr3.1:60252899-60252918 | MS.gene30740:CDS | 55.0% | |
| ! | AGTGAGGAGAGTGGTTTGGG+TGG | - | chr3.1:60252977-60252996 | MS.gene30740:CDS | 55.0% |
| CGAGTAGCACGTGGACTTCC+CGG | + | chr3.1:60252920-60252939 | None:intergenic | 60.0% | |
| GAAGACACCCGAGTAGCACG+TGG | + | chr3.1:60252929-60252948 | None:intergenic | 60.0% | |
| ! | CGGGAAGTCCACGTGCTACT+CGG | - | chr3.1:60252918-60252937 | MS.gene30740:CDS | 60.0% |
| ! | GGAGAGTGGTTTGGGTGGTG+AGG | - | chr3.1:60252982-60253001 | MS.gene30740:CDS | 60.0% |
| ! | GGGAAGTCCACGTGCTACTC+GGG | - | chr3.1:60252919-60252938 | MS.gene30740:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 60252311 | 60253237 | 60252311 | ID=MS.gene30740 |
| chr3.1 | mRNA | 60252311 | 60253237 | 60252311 | ID=MS.gene30740.t1;Parent=MS.gene30740 |
| chr3.1 | exon | 60252311 | 60253237 | 60252311 | ID=MS.gene30740.t1.exon1;Parent=MS.gene30740.t1 |
| chr3.1 | CDS | 60252311 | 60253237 | 60252311 | ID=cds.MS.gene30740.t1;Parent=MS.gene30740.t1 |
| Gene Sequence |
| Protein sequence |
