AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31137

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31137.t1 MTR_2g097230 96.503 286 10 0 1 286 1 286 0.0 575
MS.gene31137.t1 MTR_4g064030 45.517 145 39 3 127 269 1 107 6.73e-34 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31137.t1 AT5G59400 57.931 290 111 4 7 285 6 295 7.43e-117 338
MS.gene31137.t1 AT5G59400 54.237 236 97 4 7 231 6 241 1.10e-81 247

Find 76 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAACGGTTGTTTCTGATTT+TGG 0.096153 2.4:+6422598 None:intergenic
TATCAAAATGCATTTAATTT+AGG 0.174239 2.4:-6421402 MS.gene31137:CDS
TTTGGTAGACAATGGGTTTA+TGG 0.211478 2.4:-6420180 MS.gene31137:CDS
ACACATGGCACTTTGCTCTA+TGG 0.252132 2.4:+6420703 None:intergenic
CTAACCTCTTCTCCACAATT+TGG 0.256151 2.4:+6421107 None:intergenic
CCATGTGTGTGAATGCTTAT+TGG 0.266944 2.4:-6420688 MS.gene31137:CDS
AAAGAGCTTCAAGGGTTTCT+TGG 0.284671 2.4:+6422480 None:intergenic
CAGCAATCAACTTCAAGATT+TGG 0.317400 2.4:-6420198 MS.gene31137:CDS
CCGACAGTTAAAACACTTCA+AGG 0.341879 2.4:-6421170 MS.gene31137:intron
TTACAGCTCCCTTTGGCGTC+TGG 0.345446 2.4:+6420134 None:intergenic
TTGGTAGACAATGGGTTTAT+GGG 0.350926 2.4:-6420179 MS.gene31137:CDS
TTTCATCTTCCTTCTCTCTT+CGG 0.366302 2.4:-6422574 MS.gene31137:CDS
GGTGAATACCATTATCTTCA+TGG 0.383957 2.4:-6421334 MS.gene31137:CDS
CCTTGAAGCTCTTTATTCTC+AGG 0.389886 2.4:-6422471 MS.gene31137:intron
CACATGGCACTTTGCTCTAT+GGG 0.391842 2.4:+6420704 None:intergenic
AAGAGCTTCAAGGGTTTCTT+GGG 0.410172 2.4:+6422481 None:intergenic
ACCGAGTGCCATGAAGATAA+TGG 0.411246 2.4:+6421326 None:intergenic
CCTGAGAATAAAGAGCTTCA+AGG 0.416387 2.4:+6422471 None:intergenic
AGAAAATTTACAGCTCCCTT+TGG 0.418396 2.4:+6420127 None:intergenic
GAATGACCTGGCAGCACTAA+AGG 0.428763 2.4:-6421138 MS.gene31137:CDS
TTACCTGAGGCAGAAGCAAC+CGG 0.444107 2.4:+6421288 None:intergenic
AGCAACAGCAGCACCGCTTT+AGG 0.444700 2.4:-6422644 MS.gene31137:CDS
TATCCGGTTGCTTCTGCCTC+AGG 0.446058 2.4:-6421291 MS.gene31137:intron
TTGGTAGTTCAGCAATGGTT+GGG 0.456189 2.4:-6421452 MS.gene31137:CDS
TTCAAGATTTGGTAGACAAT+GGG 0.459540 2.4:-6420187 MS.gene31137:CDS
TTTGGTAGTTCAGCAATGGT+TGG 0.463462 2.4:-6421453 MS.gene31137:CDS
GAGGACTTAGTTGTGATGCT+TGG 0.477149 2.4:+6421364 None:intergenic
TGCATTTGGTAGTTCAGCAA+TGG 0.480534 2.4:-6421457 MS.gene31137:CDS
GGTAACAGTTGAAGCTTAAG+TGG 0.481788 2.4:-6422117 MS.gene31137:intron
TTTAATTTAGGATTATCATA+TGG 0.482685 2.4:-6421390 MS.gene31137:CDS
CTTCAAGATTTGGTAGACAA+TGG 0.486758 2.4:-6420188 MS.gene31137:CDS
CAGTTGAAGCTTAAGTGGTA+TGG 0.493314 2.4:-6422112 MS.gene31137:CDS
AGGGCAGGAAGATCTGTCTA+TGG 0.493392 2.4:-6421514 MS.gene31137:intron
CTGAGAATAAAGAGCTTCAA+GGG 0.499082 2.4:+6422472 None:intergenic
ACCATTATCTTCATGGCACT+CGG 0.500659 2.4:-6421327 MS.gene31137:CDS
GCGTAAATCCAGACGCCAAA+GGG 0.509490 2.4:-6420142 MS.gene31137:CDS
AATGACCTGGCAGCACTAAA+GGG 0.512532 2.4:-6421137 MS.gene31137:CDS
GGTCCATCTTGTATATATGT+TGG 0.512587 2.4:-6422521 MS.gene31137:CDS
TTAAAACACTTCAAGGCTTG+TGG 0.533051 2.4:-6421163 MS.gene31137:CDS
TTATGTTATTACCTCAACTT+CGG 0.535679 2.4:+6420656 None:intergenic
AGGATACTTCACAACTGACT+TGG 0.542875 2.4:+6422087 None:intergenic
TCTTGGGTGGCAGTTTGAAG+AGG 0.543205 2.4:+6422497 None:intergenic
GGAATTTCGCACCGAAGTTG+AGG 0.547885 2.4:-6420667 MS.gene31137:intron
ACCTGCGACGACCGTCCTCA+TGG 0.549456 2.4:-6422542 MS.gene31137:CDS
AACATGTCATCAACTATCAA+AGG 0.550328 2.4:+6422237 None:intergenic
GGCAGAGATCCGAAGAGAGA+AGG 0.551019 2.4:+6422565 None:intergenic
ACCATGAGGACGGTCGTCGC+AGG 0.551432 2.4:+6422541 None:intergenic
GCAGCACCGCTTTAGGTGGA+TGG 0.553063 2.4:-6422637 MS.gene31137:CDS
GGACCAACATATATACAAGA+TGG 0.557422 2.4:+6422518 None:intergenic
ATGAACCCTTTAGTGCTGCC+AGG 0.558508 2.4:+6421132 None:intergenic
AGCTTCAAGGGTTTCTTGGG+TGG 0.559365 2.4:+6422484 None:intergenic
ATGGTATTCACCATGAAAAG+AGG 0.559608 2.4:+6421345 None:intergenic
ATGTCCAAATTGTGGAGAAG+AGG 0.561187 2.4:-6421111 MS.gene31137:intron
AAGGGTTCATGTCCAAATTG+TGG 0.563160 2.4:-6421119 MS.gene31137:CDS
AGGCTTGTGGAGGAATGACC+TGG 0.576931 2.4:-6421150 MS.gene31137:CDS
CACCGTCCATCCACCTAAAG+CGG 0.577414 2.4:+6422631 None:intergenic
CACCGCTTTAGGTGGATGGA+CGG 0.580229 2.4:-6422633 MS.gene31137:CDS
TGCGTAAATCCAGACGCCAA+AGG 0.585411 2.4:-6420143 MS.gene31137:CDS
TGCATTTGTAAGAACAGACA+AGG 0.588695 2.4:-6420739 MS.gene31137:CDS
AAACACTTCAAGGCTTGTGG+AGG 0.598739 2.4:-6421160 MS.gene31137:CDS
ATGAGGACGGTCGTCGCAGG+TGG 0.602794 2.4:+6422544 None:intergenic
AAGTTGATGCTGCAAAACAT+TGG 0.603833 2.4:+6421429 None:intergenic
ACAAGCACATATGAACATGT+TGG 0.606754 2.4:-6422675 None:intergenic
AACAGCAGCACCGCTTTAGG+TGG 0.608229 2.4:-6422641 MS.gene31137:CDS
ATCCTCGTTGCAGTATCAGA+AGG 0.615303 2.4:-6422069 MS.gene31137:CDS
CCAATAAGCATTCACACACA+TGG 0.617576 2.4:+6420688 None:intergenic
AATCACCTCAGCATCAGCAT+AGG 0.620614 2.4:+6422039 None:intergenic
TGACATGTTTGATAGAGTAG+AGG 0.624434 2.4:-6422223 MS.gene31137:intron
GCAAGGGATGCATATTACAA+AGG 0.629849 2.4:-6422264 MS.gene31137:CDS
GGCGTCTGGATTTACGCACA+AGG 0.641633 2.4:+6420148 None:intergenic
TACAAGATGGACCATGAGGA+CGG 0.641757 2.4:+6422531 None:intergenic
ATCAACCTATGCTGATGCTG+AGG 0.651620 2.4:-6422044 MS.gene31137:intron
ACATGGCACTTTGCTCTATG+GGG 0.653009 2.4:+6420705 None:intergenic
AGAAGGAAGATGAAACAGAA+CGG 0.670572 2.4:+6422582 None:intergenic
TATATACAAGATGGACCATG+AGG 0.716840 2.4:+6422527 None:intergenic
TGCCTTCTGATACTGCAACG+AGG 0.764708 2.4:+6422067 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAAAGTTAAAATTTGTA+AGG + chr2.4:6421568-6421587 None:intergenic 10.0%
!! TTAAAGAAAAAATAATACTT+TGG - chr2.4:6422297-6422316 MS.gene31137:intron 10.0%
!!! ATTTTAATAAAACTATTTTC+AGG + chr2.4:6421026-6421045 None:intergenic 10.0%
!!! GAAAATAGTTTTATTAAAAT+TGG - chr2.4:6421026-6421045 MS.gene31137:intron 10.0%
!! ATCTTATGTTATTCATATTA+TGG - chr2.4:6420661-6420680 MS.gene31137:intron 15.0%
!! GTAACATAATATTAATTGAA+GGG - chr2.4:6422327-6422346 MS.gene31137:intron 15.0%
!! TAATAAATATTACATTCATC+TGG - chr2.4:6421173-6421192 MS.gene31137:intron 15.0%
!! TAATCATATGTGAAATTAAA+TGG + chr2.4:6422503-6422522 None:intergenic 15.0%
!! TGTAACATAATATTAATTGA+AGG - chr2.4:6422326-6422345 MS.gene31137:intron 15.0%
!!! ATTGAGAATTATTTTAAACA+TGG - chr2.4:6420491-6420510 MS.gene31137:intron 15.0%
!!! CATTTTGAAACAATTTTTTT+TGG - chr2.4:6422401-6422420 MS.gene31137:intron 15.0%
!!! TATCAAAATGCATTTAATTT+AGG - chr2.4:6421397-6421416 MS.gene31137:CDS 15.0%
!!! TTTAATTTAGGATTATCATA+TGG - chr2.4:6421409-6421428 MS.gene31137:CDS 15.0%
!!! TTTTATTAAAATTGGTTTTG+CGG - chr2.4:6421034-6421053 MS.gene31137:intron 15.0%
!! AAATTATCCTAATCAACTAT+TGG - chr2.4:6422263-6422282 MS.gene31137:CDS 20.0%
!! AATAGTTGATTAGGATAATT+TGG + chr2.4:6422264-6422283 None:intergenic 20.0%
!! GAATAACATAAGATTAAGAT+CGG + chr2.4:6420655-6420674 None:intergenic 20.0%
!!! GTTGTTTGTTTTATTGAAAA+AGG - chr2.4:6421853-6421872 MS.gene31137:intron 20.0%
! ACTACCAAATGCAAAAAATA+AGG + chr2.4:6421335-6421354 None:intergenic 25.0%
! ATAATGCAAAGAATGCATAT+TGG - chr2.4:6420926-6420945 MS.gene31137:intron 25.0%
! TAAGTATAAGCAACTGAAAT+TGG + chr2.4:6420852-6420871 None:intergenic 25.0%
! TAATGCAAAGAATGCATATT+GGG - chr2.4:6420927-6420946 MS.gene31137:intron 25.0%
! TATGAAAGACTACAAAGTTT+GGG + chr2.4:6421726-6421745 None:intergenic 25.0%
! TTATGTTATTACCTCAACTT+CGG + chr2.4:6422146-6422165 None:intergenic 25.0%
!! TGGTGAATTTTCATCAATAA+GGG + chr2.4:6420832-6420851 None:intergenic 25.0%
!! TTGGTGAATTTTCATCAATA+AGG + chr2.4:6420833-6420852 None:intergenic 25.0%
!!! ATACTTAAGTTTTAGTTGCA+TGG - chr2.4:6420865-6420884 MS.gene31137:intron 25.0%
!!! ATTGTTTTACTTCTTGCAAA+AGG - chr2.4:6422457-6422476 MS.gene31137:intron 25.0%
!!! GAATGTTTTTGTTTTCGAAA+AGG - chr2.4:6421085-6421104 MS.gene31137:intron 25.0%
!!! GAATTATTTTAAACATGGCA+TGG - chr2.4:6420496-6420515 MS.gene31137:intron 25.0%
!!! TTCAAAATGAATGTCACTTT+CGG + chr2.4:6422390-6422409 None:intergenic 25.0%
AAAAGGCAGTTCATAACATT+AGG - chr2.4:6422474-6422493 MS.gene31137:CDS 30.0%
AACATGTCATCAACTATCAA+AGG + chr2.4:6420565-6420584 None:intergenic 30.0%
AATGCAAAGAATGCATATTG+GGG - chr2.4:6420928-6420947 MS.gene31137:intron 30.0%
ATATGAAAAGTAGCAGCAAA+AGG + chr2.4:6421541-6421560 None:intergenic 30.0%
ATATTGAATGATGTGGATGT+AGG - chr2.4:6421265-6421284 MS.gene31137:intron 30.0%
ATGAAAGACTACAAAGTTTG+GGG + chr2.4:6421725-6421744 None:intergenic 30.0%
CAACAAACCAATAGTTGATT+AGG + chr2.4:6422273-6422292 None:intergenic 30.0%
CAAGAATAGAGAAAAGAGAA+GGG + chr2.4:6421231-6421250 None:intergenic 30.0%
CGTCTTAATATTGAATGATG+TGG - chr2.4:6421258-6421277 MS.gene31137:intron 30.0%
CTATGAAAGACTACAAAGTT+TGG + chr2.4:6421727-6421746 None:intergenic 30.0%
CTTTGACTAATGATCTTGTT+TGG - chr2.4:6421768-6421787 MS.gene31137:intron 30.0%
GCATTCTTTGCATTATTAAG+AGG + chr2.4:6420922-6420941 None:intergenic 30.0%
TATTGAATGATGTGGATGTA+GGG - chr2.4:6421266-6421285 MS.gene31137:intron 30.0%
TTAAAATTGTCTGAGTGAGT+TGG - chr2.4:6422016-6422035 MS.gene31137:intron 30.0%
TTCTTACAAATGCAAATACC+TGG + chr2.4:6422054-6422073 None:intergenic 30.0%
TTGCTTATCGAATAAGTGTT+CGG + chr2.4:6421807-6421826 None:intergenic 30.0%
! AAGAAGTTAAGAAGCATATG+TGG + chr2.4:6420623-6420642 None:intergenic 30.0%
! TTCAAGATTTGGTAGACAAT+GGG - chr2.4:6422612-6422631 MS.gene31137:CDS 30.0%
!! AAGCGACACTCATTTTTAAA+CGG + chr2.4:6422181-6422200 None:intergenic 30.0%
!!! TGGACCTTATTTTTTGCATT+TGG - chr2.4:6421328-6421347 MS.gene31137:CDS 30.0%
!!! TGTTTTAACTGTCGGAAAAA+AGG + chr2.4:6421624-6421643 None:intergenic 30.0%
!!! TTTTCAGGTCTAATCAATGT+AGG + chr2.4:6421011-6421030 None:intergenic 30.0%
AATGATGTTGGATCTTCATG+TGG - chr2.4:6420953-6420972 MS.gene31137:intron 35.0%
AGAAAAGAGAAGGGAAAAAG+GGG + chr2.4:6421222-6421241 None:intergenic 35.0%
AGAAGGAAGATGAAACAGAA+CGG + chr2.4:6420220-6420239 None:intergenic 35.0%
AGAGAAAAGAGAAGGGAAAA+AGG + chr2.4:6421224-6421243 None:intergenic 35.0%
ATGCAAATACCTGGAAGATA+AGG + chr2.4:6422045-6422064 None:intergenic 35.0%
ATGGTATTCACCATGAAAAG+AGG + chr2.4:6421457-6421476 None:intergenic 35.0%
CAAAGAAGCCAAAGAAGTTA+AGG + chr2.4:6421753-6421772 None:intergenic 35.0%
CAGCAATCAACTTCAAGATT+TGG - chr2.4:6422601-6422620 MS.gene31137:CDS 35.0%
CATAGTCTCCTTAACTTCTT+TGG - chr2.4:6421742-6421761 MS.gene31137:intron 35.0%
CCAAGAATAGAGAAAAGAGA+AGG + chr2.4:6421232-6421251 None:intergenic 35.0%
CTGAGAATAAAGAGCTTCAA+GGG + chr2.4:6420330-6420349 None:intergenic 35.0%
GAGAAAAGAGAAGGGAAAAA+GGG + chr2.4:6421223-6421242 None:intergenic 35.0%
GCGCTTATGCTTAATTAAGT+TGG - chr2.4:6420418-6420437 MS.gene31137:intron 35.0%
GGACCAACATATATACAAGA+TGG + chr2.4:6420284-6420303 None:intergenic 35.0%
GGTCCATCTTGTATATATGT+TGG - chr2.4:6420278-6420297 MS.gene31137:intron 35.0%
GGTGAATACCATTATCTTCA+TGG - chr2.4:6421465-6421484 MS.gene31137:CDS 35.0%
TATATACAAGATGGACCATG+AGG + chr2.4:6420275-6420294 None:intergenic 35.0%
TATGACAGTTAGCAATCTAG+AGG - chr2.4:6421911-6421930 MS.gene31137:intron 35.0%
TGAAAGACTACAAAGTTTGG+GGG + chr2.4:6421724-6421743 None:intergenic 35.0%
TGACATGTTTGATAGAGTAG+AGG - chr2.4:6420576-6420595 MS.gene31137:intron 35.0%
TGATCTTTGCTAATAGGCAA+GGG - chr2.4:6420519-6420538 MS.gene31137:intron 35.0%
TGCATTTGTAAGAACAGACA+AGG - chr2.4:6422060-6422079 MS.gene31137:CDS 35.0%
TTAAAACACTTCAAGGCTTG+TGG - chr2.4:6421636-6421655 MS.gene31137:intron 35.0%
TTCCTTAATCTGTGTGCAAT+TGG + chr2.4:6422207-6422226 None:intergenic 35.0%
TTTCATCTTCCTTCTCTCTT+CGG - chr2.4:6420225-6420244 MS.gene31137:intron 35.0%
! AAGAAGCATATGTGGTGAAT+TGG + chr2.4:6420615-6420634 None:intergenic 35.0%
! AATGTTTTGCAGCATCAACT+TGG - chr2.4:6421372-6421391 MS.gene31137:CDS 35.0%
! ACAACTAAGTCCTCTTTTCA+TGG - chr2.4:6421444-6421463 MS.gene31137:CDS 35.0%
! AGAAGCATATGTGGTGAATT+GGG + chr2.4:6420614-6420633 None:intergenic 35.0%
! AGCAAAAGGATTTTTACCTG+AGG + chr2.4:6421527-6421546 None:intergenic 35.0%
! CCTTCTCTTTTCTCTATTCT+TGG - chr2.4:6421229-6421248 MS.gene31137:intron 35.0%
! CTTCAAGATTTGGTAGACAA+TGG - chr2.4:6422611-6422630 MS.gene31137:CDS 35.0%
! TTCATCTGGTTAGAGTGTTA+AGG - chr2.4:6421187-6421206 MS.gene31137:intron 35.0%
! TTGGTAGACAATGGGTTTAT+GGG - chr2.4:6422620-6422639 MS.gene31137:CDS 35.0%
! TTTGGTAGACAATGGGTTTA+TGG - chr2.4:6422619-6422638 MS.gene31137:CDS 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr2.4:6422427-6422446 MS.gene31137:intron 35.0%
!! AAGTTGATGCTGCAAAACAT+TGG + chr2.4:6421373-6421392 None:intergenic 35.0%
!! GACACTCATTTTTAAACGGA+GGG + chr2.4:6422177-6422196 None:intergenic 35.0%
!!! AGAACGGTTGTTTCTGATTT+TGG + chr2.4:6420204-6420223 None:intergenic 35.0%
!!! CCTTGAAGTGTTTTAACTGT+CGG + chr2.4:6421632-6421651 None:intergenic 35.0%
AAAGAGCTTCAAGGGTTTCT+TGG + chr2.4:6420322-6420341 None:intergenic 40.0%
AAGAGCTTCAAGGGTTTCTT+GGG + chr2.4:6420321-6420340 None:intergenic 40.0%
AAGCATATGTGGTGAATTGG+GGG + chr2.4:6420612-6420631 None:intergenic 40.0%
AAGGGTTCATGTCCAAATTG+TGG - chr2.4:6421680-6421699 MS.gene31137:intron 40.0%
ACCATTATCTTCATGGCACT+CGG - chr2.4:6421472-6421491 MS.gene31137:CDS 40.0%
AGCCAATTGCACACAGATTA+AGG - chr2.4:6422202-6422221 MS.gene31137:intron 40.0%
AGGATACTTCACAACTGACT+TGG + chr2.4:6420715-6420734 None:intergenic 40.0%
AGTTGGATTCCTTATCTTCC+AGG - chr2.4:6422033-6422052 MS.gene31137:intron 40.0%
ATCAATAAGGGTCATAGCCA+AGG + chr2.4:6420820-6420839 None:intergenic 40.0%
ATGTCCAAATTGTGGAGAAG+AGG - chr2.4:6421688-6421707 MS.gene31137:intron 40.0%
CAGTTGAAGCTTAAGTGGTA+TGG - chr2.4:6420687-6420706 MS.gene31137:CDS 40.0%
CATATTGGGGAGAATGATGT+TGG - chr2.4:6420941-6420960 MS.gene31137:intron 40.0%
CCAATAAGCATTCACACACA+TGG + chr2.4:6422114-6422133 None:intergenic 40.0%
CCATGTGTGTGAATGCTTAT+TGG - chr2.4:6422111-6422130 MS.gene31137:CDS 40.0%
CCGACAGTTAAAACACTTCA+AGG - chr2.4:6421629-6421648 MS.gene31137:intron 40.0%
CCTGAGAATAAAGAGCTTCA+AGG + chr2.4:6420331-6420350 None:intergenic 40.0%
CCTTGAAGCTCTTTATTCTC+AGG - chr2.4:6420328-6420347 MS.gene31137:intron 40.0%
CTAACCTCTTCTCCACAATT+TGG + chr2.4:6421695-6421714 None:intergenic 40.0%
GAAAAGAGAAGGGAAAAAGG+GGG + chr2.4:6421221-6421240 None:intergenic 40.0%
GAAGCATATGTGGTGAATTG+GGG + chr2.4:6420613-6420632 None:intergenic 40.0%
GCAAGGGATGCATATTACAA+AGG - chr2.4:6420535-6420554 MS.gene31137:intron 40.0%
GCATGGTGATCTTTGCTAAT+AGG - chr2.4:6420513-6420532 MS.gene31137:intron 40.0%
GGTAACAGTTGAAGCTTAAG+TGG - chr2.4:6420682-6420701 MS.gene31137:CDS 40.0%
GTGATCTTTGCTAATAGGCA+AGG - chr2.4:6420518-6420537 MS.gene31137:intron 40.0%
! TGCATTTGGTAGTTCAGCAA+TGG - chr2.4:6421342-6421361 MS.gene31137:CDS 40.0%
! TTGGTAGTTCAGCAATGGTT+GGG - chr2.4:6421347-6421366 MS.gene31137:CDS 40.0%
! TTTGGTAGTTCAGCAATGGT+TGG - chr2.4:6421346-6421365 MS.gene31137:CDS 40.0%
! TTTTTACATTAGCGAGTGCG+TGG - chr2.4:6421308-6421327 MS.gene31137:CDS 40.0%
!! CGACACTCATTTTTAAACGG+AGG + chr2.4:6422178-6422197 None:intergenic 40.0%
!! CGGTTTTGTCATAGGATATC+CGG - chr2.4:6421492-6421511 MS.gene31137:CDS 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr2.4:6422431-6422450 MS.gene31137:intron 40.0%
AAACACTTCAAGGCTTGTGG+AGG - chr2.4:6421639-6421658 MS.gene31137:intron 45.0%
AATCACCTCAGCATCAGCAT+AGG + chr2.4:6420763-6420782 None:intergenic 45.0%
AATGACCTGGCAGCACTAAA+GGG - chr2.4:6421662-6421681 MS.gene31137:intron 45.0%
ACACATGGCACTTTGCTCTA+TGG + chr2.4:6422099-6422118 None:intergenic 45.0%
ACATGGCACTTTGCTCTATG+GGG + chr2.4:6422097-6422116 None:intergenic 45.0%
ACCGAGTGCCATGAAGATAA+TGG + chr2.4:6421476-6421495 None:intergenic 45.0%
ACTTCTTCTTTGCTCTGCCT+TGG - chr2.4:6420800-6420819 MS.gene31137:intron 45.0%
ATCAACCTATGCTGATGCTG+AGG - chr2.4:6420755-6420774 MS.gene31137:intron 45.0%
ATCCTCGTTGCAGTATCAGA+AGG - chr2.4:6420730-6420749 MS.gene31137:CDS 45.0%
CACATGGCACTTTGCTCTAT+GGG + chr2.4:6422098-6422117 None:intergenic 45.0%
GAGGACTTAGTTGTGATGCT+TGG + chr2.4:6421438-6421457 None:intergenic 45.0%
TACAAGATGGACCATGAGGA+CGG + chr2.4:6420271-6420290 None:intergenic 45.0%
!! ATGGCACTCGGTTTTGTCAT+AGG - chr2.4:6421484-6421503 MS.gene31137:CDS 45.0%
!! TGGTTTTGCGGCGTTGAATT+AGG - chr2.4:6421046-6421065 MS.gene31137:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr2.4:6422430-6422449 MS.gene31137:intron 45.0%
AGAAGGGAAAAAGGGGGTAG+GGG + chr2.4:6421215-6421234 None:intergenic 50.0%
AGAGAAGGGAAAAAGGGGGT+AGG + chr2.4:6421217-6421236 None:intergenic 50.0%
AGCTTCAAGGGTTTCTTGGG+TGG + chr2.4:6420318-6420337 None:intergenic 50.0%
AGGGCAGGAAGATCTGTCTA+TGG - chr2.4:6421285-6421304 MS.gene31137:intron 50.0%
ATGAACCCTTTAGTGCTGCC+AGG + chr2.4:6421670-6421689 None:intergenic 50.0%
GAATGACCTGGCAGCACTAA+AGG - chr2.4:6421661-6421680 MS.gene31137:intron 50.0%
GAATGATGTGGATGTAGGGC+AGG - chr2.4:6421270-6421289 MS.gene31137:intron 50.0%
GAGAAGGGAAAAAGGGGGTA+GGG + chr2.4:6421216-6421235 None:intergenic 50.0%
GCGTAAATCCAGACGCCAAA+GGG - chr2.4:6422657-6422676 MS.gene31137:CDS 50.0%
GGAATTTCGCACCGAAGTTG+AGG - chr2.4:6422132-6422151 MS.gene31137:intron 50.0%
TCTTGGGTGGCAGTTTGAAG+AGG + chr2.4:6420305-6420324 None:intergenic 50.0%
TGCCTTCTGATACTGCAACG+AGG + chr2.4:6420735-6420754 None:intergenic 50.0%
TGCGTAAATCCAGACGCCAA+AGG - chr2.4:6422656-6422675 MS.gene31137:CDS 50.0%
TTACCTGAGGCAGAAGCAAC+CGG + chr2.4:6421514-6421533 None:intergenic 50.0%
AGCAACAGCAGCACCGCTTT+AGG - chr2.4:6420155-6420174 MS.gene31137:CDS 55.0%
AGGCTTGTGGAGGAATGACC+TGG - chr2.4:6421649-6421668 MS.gene31137:intron 55.0%
CACCGTCCATCCACCTAAAG+CGG + chr2.4:6420171-6420190 None:intergenic 55.0%
GGCAGAGATCCGAAGAGAGA+AGG + chr2.4:6420237-6420256 None:intergenic 55.0%
GGCGTCTGGATTTACGCACA+AGG + chr2.4:6422654-6422673 None:intergenic 55.0%
TATCCGGTTGCTTCTGCCTC+AGG - chr2.4:6421508-6421527 MS.gene31137:CDS 55.0%
TTACAGCTCCCTTTGGCGTC+TGG + chr2.4:6422668-6422687 None:intergenic 55.0%
! AACAGCAGCACCGCTTTAGG+TGG - chr2.4:6420158-6420177 MS.gene31137:CDS 55.0%
! CACCGCTTTAGGTGGATGGA+CGG - chr2.4:6420166-6420185 MS.gene31137:CDS 55.0%
! GCAGCACCGCTTTAGGTGGA+TGG - chr2.4:6420162-6420181 MS.gene31137:CDS 60.0%
ACCATGAGGACGGTCGTCGC+AGG + chr2.4:6420261-6420280 None:intergenic 65.0%
ACCTGCGACGACCGTCCTCA+TGG - chr2.4:6420257-6420276 MS.gene31137:intron 65.0%
ATGAGGACGGTCGTCGCAGG+TGG + chr2.4:6420258-6420277 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 6420134 6422687 6420134 ID=MS.gene31137
chr2.4 mRNA 6420134 6422687 6420134 ID=MS.gene31137.t1;Parent=MS.gene31137
chr2.4 exon 6422472 6422687 6422472 ID=MS.gene31137.t1.exon1;Parent=MS.gene31137.t1
chr2.4 CDS 6422472 6422687 6422472 ID=cds.MS.gene31137.t1;Parent=MS.gene31137.t1
chr2.4 exon 6422224 6422286 6422224 ID=MS.gene31137.t1.exon2;Parent=MS.gene31137.t1
chr2.4 CDS 6422224 6422286 6422224 ID=cds.MS.gene31137.t1;Parent=MS.gene31137.t1
chr2.4 exon 6422045 6422131 6422045 ID=MS.gene31137.t1.exon3;Parent=MS.gene31137.t1
chr2.4 CDS 6422045 6422131 6422045 ID=cds.MS.gene31137.t1;Parent=MS.gene31137.t1
chr2.4 exon 6421292 6421529 6421292 ID=MS.gene31137.t1.exon4;Parent=MS.gene31137.t1
chr2.4 CDS 6421292 6421529 6421292 ID=cds.MS.gene31137.t1;Parent=MS.gene31137.t1
chr2.4 exon 6421112 6421185 6421112 ID=MS.gene31137.t1.exon5;Parent=MS.gene31137.t1
chr2.4 CDS 6421112 6421185 6421112 ID=cds.MS.gene31137.t1;Parent=MS.gene31137.t1
chr2.4 exon 6420668 6420766 6420668 ID=MS.gene31137.t1.exon6;Parent=MS.gene31137.t1
chr2.4 CDS 6420668 6420766 6420668 ID=cds.MS.gene31137.t1;Parent=MS.gene31137.t1
chr2.4 exon 6420134 6420217 6420134 ID=MS.gene31137.t1.exon7;Parent=MS.gene31137.t1
chr2.4 CDS 6420134 6420217 6420134 ID=cds.MS.gene31137.t1;Parent=MS.gene31137.t1
Gene Sequence

>MS.gene31137

ATGAACATGTTGGCAAGCACGAGCAACAGCAGCACCGCTTTAGGTGGATGGACGGTGTTACAGCTATCCAAAATCAGAAACAACCGTTCTGTTTCATCTTCCTTCTCTCTTCGGATCTCTGCCACCTGCGACGACCGTCCTCATGGTCCATCTTGTATATATGTTGGTCCTCTTCAAACTGCCACCCAAGAAACCCTTGAAGCTCTTTATTCTCAGGTAACTTCTATTGAGCTTTTCTCGCAAATTCACTTGATAATAATACTGCTTCTGATTTTATGCATAAAGCGCTTATGCTTAATTAAGTTGGTTATGTAATCAGTATTTTTATCTTTCTTCTTTTCATGTATTTATTTCTCTATTGAGAATTATTTTAAACATGGCATGGTGATCTTTGCTAATAGGCAAGGGATGCATATTACAAAGGAGAGCCTTTGATAGTTGATGACATGTTTGATAGAGTAGAGGTATCATTCTTCCCCCAATTCACCACATATGCTTCTTAACTTCTTATATGTATACCGATCTTAATCTTATGTTATTCATATTATGGTAACAGTTGAAGCTTAAGTGGTATGGTTCCAAGTCAGTTGTGAAGTATCCTCGTTGCAGTATCAGAAGGCAATCAACCTATGCTGATGCTGAGGTGATTTTCTTCAGTTTAGATTAACTTCTTCTTTGCTCTGCCTTGGCTATGACCCTTATTGATGAAAATTCACCAATTTCAGTTGCTTATACTTAAGTTTTAGTTGCATGGTGCTCTTAATATTGTTTTTTTTTAAACGTGTCCTCTTAATAATGCAAAGAATGCATATTGGGGAGAATGATGTTGGATCTTCATGTGGCTGAGAAAGTTATTTGTGTGAAAAATTCAAATCCTACATTGATTAGACCTGAAAATAGTTTTATTAAAATTGGTTTTGCGGCGTTGAATTAGGTTCACGAACAAATATTGAATGTTTTTGTTTTCGAAAAGGCATTATGTTCATGTTTTGTGATATGTTCTATTTGTGTTGAATGTCTCTAGTTTCTACATGTTTTGTAATAAATATTACATTCATCTGGTTAGAGTGTTAAGGTTCCCCTACCCCCTTTTTCCCTTCTCTTTTCTCTATTCTTGGTTTCTTCGTCTTAATATTGAATGATGTGGATGTAGGGCAGGAAGATCTGTCTATGGTTTTTACATTAGCGAGTGCGTGGACCTTATTTTTTGCATTTGGTAGTTCAGCAATGGTTGGGCCAATGTTTTGCAGCATCAACTTGGCTTATCAAAATGCATTTAATTTAGGATTATCATATGGTAGCCAAGCATCACAACTAAGTCCTCTTTTCATGGTGAATACCATTATCTTCATGGCACTCGGTTTTGTCATAGGATATCCGGTTGCTTCTGCCTCAGGTAAAAATCCTTTTGCTGCTACTTTTCATATTAAGCCTTACAAATTTTAACTTTTTATGTCACTGCTTCTATATTCATATGTTATTACGTGCCTTTTTTCCGACAGTTAAAACACTTCAAGGCTTGTGGAGGAATGACCTGGCAGCACTAAAGGGTTCATGTCCAAATTGTGGAGAAGAGGTTAGTCATTGCCCCCAAACTTTGTAGTCTTTCATAGTCTCCTTAACTTCTTTGGCTTCTTTGACTAATGATCTTGTTTGGATACACATCTTAACCGAACACTTATTCGATAAGCAATTATCTTATTATGAGCTTATATAGCAGTTGTTTGTTTTATTGAAAAAGGAATGAGAGTGAACTTTATATAATCTAAAAACAATTTATGACAGTTAGCAATCTAGAGGAACTTCAATAAGTTATAAGCAACTTATGAATATATCATTAACTTTTTCATAAGTTCTTTCAAACACTTGCACAAGAACTTCATTAAAATTGTCTGAGTGAGTTGGATTCCTTATCTTCCAGGTATTTGCATTTGTAAGAACAGACAAGGCTAATAACTCACCCCATAGAGCAAAGTGCCATGTGTGTGAATGCTTATTGGAATTTCGCACCGAAGTTGAGGTAATAACATAACTAATACTCCCTCCGTTTAAAAATGAGTGTCGCTTTAGCCAATTGCACACAGATTAAGGAATGAAATGAAGTAGTCAAATGTCATAGCAATTTTACCAAATTATCCTAATCAACTATTGGTTTGTTGTTCATTAAAGAAAAAATAATACTTTGGAAGTAGTGTAACATAATATTAATTGAAGGGTACAATTGAAAAGAAAAAGTTATTATTGCATTGAAAACCGAAAGTGACATTCATTTTGAAACAATTTTTTTTGGCTAAAGCGACACTCATTTTGAAACGGAGGGAGTATTGTTTTACTTCTTGCAAAAGGCAGTTCATAACATTAGGCTGCCATTTAATTTCACATATGATTATGAGTATAAAAACATATAGTATAGATCAAGAGCTGCTAGTATTTTTCTCTTTTGCATGACACATGATGTTATCATTTGCAGCAATCAACTTCAAGATTTGGTAGACAATGGGTTTATGGGCGTATTTACCTTGTGCGTAAATCCAGACGCCAAAGGGAGCTGTAA
Protein sequence

>MS.gene31137.t1

MNMLASTSNSSTALGGWTVLQLSKIRNNRSVSSSFSLRISATCDDRPHGPSCIYVGPLQTATQETLEALYSQARDAYYKGEPLIVDDMFDRVELKLKWYGSKSVVKYPRCSIRRQSTYADAEEDLSMVFTLASAWTLFFAFGSSAMVGPMFCSINLAYQNAFNLGLSYGSQASQLSPLFMVNTIIFMALGFVIGYPVASASVKTLQGLWRNDLAALKGSCPNCGEEVFAFVRTDKANNSPHRAKCHVCECLLEFRTEVEQSTSRFGRQWVYGRIYLVRKSRRQREL