AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31224

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31224.t1 MTR_2g095730 94.923 453 23 0 6 458 56 508 0.0 902
MS.gene31224.t1 MTR_2g095750 82.045 440 76 2 7 446 63 499 0.0 761
MS.gene31224.t1 MTR_2g095710 78.912 441 79 5 6 446 56 482 0.0 700
MS.gene31224.t1 MTR_4g057595 65.438 434 141 6 16 447 70 496 0.0 592
MS.gene31224.t1 MTR_4g057585 63.964 444 150 6 7 446 57 494 0.0 591
MS.gene31224.t1 MTR_1g094155 62.527 459 158 6 8 457 46 499 0.0 591
MS.gene31224.t1 MTR_4g057600 62.198 455 154 7 5 447 46 494 0.0 579
MS.gene31224.t1 MTR_1g050545 64.815 432 143 6 19 446 56 482 0.0 559
MS.gene31224.t1 MTR_4g057570 57.424 458 170 11 5 446 43 491 9.61e-175 499
MS.gene31224.t1 MTR_5g097180 32.093 430 267 9 34 453 31 445 6.28e-68 224
MS.gene31224.t1 MTR_4g084710 32.289 415 256 8 40 445 39 437 1.04e-63 213
MS.gene31224.t1 MTR_3g079560 29.604 429 258 12 44 453 92 495 7.68e-51 179
MS.gene31224.t1 MTR_3g079570 28.764 445 280 12 22 453 75 495 1.47e-50 179
MS.gene31224.t1 MTR_3g079590 28.894 443 283 12 22 453 74 495 4.91e-50 177
MS.gene31224.t1 MTR_1g020090 29.268 451 277 13 14 446 69 495 1.21e-48 174
MS.gene31224.t1 MTR_3g434850 29.767 430 252 13 44 445 44 451 2.72e-48 172
MS.gene31224.t1 MTR_2g063000 26.350 463 285 14 16 444 19 459 1.37e-47 170
MS.gene31224.t1 MTR_1g021010 29.286 420 255 12 44 446 90 484 9.96e-47 168
MS.gene31224.t1 MTR_3g079600 27.294 425 268 10 44 449 90 492 1.88e-46 168
MS.gene31224.t1 MTR_2g018590 28.571 469 261 18 20 445 30 467 2.94e-46 167
MS.gene31224.t1 MTR_2g081620 27.700 426 262 12 44 444 55 459 4.81e-46 166
MS.gene31224.t1 MTR_2g062990 26.887 424 268 12 44 444 54 458 1.41e-45 165
MS.gene31224.t1 MTR_8g011350 26.737 475 271 11 30 444 35 492 2.04e-45 165
MS.gene31224.t1 MTR_3g034290 27.818 417 273 12 41 444 85 486 2.95e-45 164
MS.gene31224.t1 MTR_1g021020 28.019 414 267 8 44 446 92 485 3.58e-45 164
MS.gene31224.t1 MTR_8g099930 26.698 427 274 14 44 445 47 459 8.78e-45 162
MS.gene31224.t1 MTR_2g062980 26.761 426 266 11 44 444 54 458 1.56e-44 162
MS.gene31224.t1 MTR_7g021130 27.700 426 265 14 46 445 46 454 1.74e-44 162
MS.gene31224.t1 MTR_3g079620 27.215 474 282 14 1 446 58 496 9.12e-44 160
MS.gene31224.t1 MTR_7g080180 35.036 274 155 7 29 293 31 290 1.54e-43 159
MS.gene31224.t1 MTR_4g122960 26.682 431 278 14 42 444 51 471 2.07e-43 159
MS.gene31224.t1 MTR_7g051430 25.918 463 267 14 44 444 44 492 2.57e-43 159
MS.gene31224.t1 MTR_3g079630 27.494 451 279 15 22 449 74 499 2.63e-43 159
MS.gene31224.t1 MTR_4g122950 26.207 435 277 14 42 444 51 473 2.76e-43 159
MS.gene31224.t1 MTR_3g498755 26.698 427 271 14 46 445 31 442 3.42e-43 158
MS.gene31224.t1 MTR_7g080180 35.036 274 155 7 29 293 31 290 4.21e-43 159
MS.gene31224.t1 MTR_7g051430 34.902 255 147 5 44 293 44 284 4.34e-43 157
MS.gene31224.t1 MTR_2g018600 26.411 443 258 16 46 445 50 467 2.71e-42 156
MS.gene31224.t1 MTR_0003s0560 27.610 431 264 13 36 444 79 483 3.87e-42 156
MS.gene31224.t1 MTR_1g095560 28.066 424 266 16 44 445 47 453 1.87e-41 153
MS.gene31224.t1 MTR_4g075150 29.216 421 269 15 44 445 48 458 5.20e-41 152
MS.gene31224.t1 MTR_2g018630 27.907 430 261 15 46 445 53 463 6.37e-41 152
MS.gene31224.t1 MTR_4g075150 29.078 423 269 15 44 445 48 460 2.76e-40 150
MS.gene31224.t1 MTR_8g011350 37.443 219 127 4 30 243 35 248 6.22e-40 150
MS.gene31224.t1 MTR_4g089155 25.463 432 282 13 41 445 64 482 1.02e-39 149
MS.gene31224.t1 MTR_3g090130 26.608 451 271 13 36 445 22 453 1.61e-39 148
MS.gene31224.t1 MTR_1g095540 27.078 421 274 13 44 445 47 453 4.92e-39 147
MS.gene31224.t1 MTR_5g072570 26.118 425 288 13 41 445 40 458 5.01e-39 147
MS.gene31224.t1 MTR_1g095560 27.051 451 268 18 44 445 47 485 5.85e-39 147
MS.gene31224.t1 MTR_2g012850 25.935 428 277 13 44 445 49 462 9.43e-39 146
MS.gene31224.t1 MTR_2g449850 25.740 439 268 15 39 445 47 459 1.15e-38 146
MS.gene31224.t1 MTR_1g040370 27.427 412 242 15 46 445 46 412 1.39e-38 145
MS.gene31224.t1 MTR_4g021535 27.273 418 250 13 58 442 65 461 2.90e-38 145
MS.gene31224.t1 MTR_4g021510 27.830 424 238 15 58 441 68 463 3.43e-38 145
MS.gene31224.t1 MTR_4g021530 27.791 421 244 16 58 442 69 465 4.42e-38 144
MS.gene31224.t1 MTR_2g012860 25.459 436 287 13 36 445 47 470 9.18e-38 144
MS.gene31224.t1 MTR_3g090150 26.056 426 285 10 41 445 44 460 3.77e-37 142
MS.gene31224.t1 MTR_7g013790 25.527 427 275 12 46 445 51 461 3.92e-37 142
MS.gene31224.t1 MTR_3g100420 25.442 452 282 10 29 442 57 491 4.26e-37 142
MS.gene31224.t1 MTR_4g021520 27.685 419 247 18 58 442 69 465 5.49e-36 139
MS.gene31224.t1 MTR_3g034290 26.586 331 227 7 41 362 85 408 1.28e-35 136
MS.gene31224.t1 MTR_3g045770 26.226 469 271 18 17 446 21 453 1.81e-35 137
MS.gene31224.t1 MTR_4g021490 26.812 414 257 14 58 442 69 465 2.25e-35 137
MS.gene31224.t1 MTR_4g021500 26.545 437 259 14 58 457 69 480 5.52e-35 139
MS.gene31224.t1 MTR_4g021500 27.295 403 251 15 58 442 504 882 1.04e-34 138
MS.gene31224.t1 MTR_4g021500 26.545 437 259 14 58 457 69 480 6.16e-35 139
MS.gene31224.t1 MTR_4g021500 24.879 414 265 12 58 442 504 900 4.67e-34 136
MS.gene31224.t1 MTR_2g063000 23.644 461 269 14 16 444 19 428 3.90e-34 133
MS.gene31224.t1 MTR_4g021520 38.537 205 115 7 46 243 56 256 1.86e-32 128
MS.gene31224.t1 MTR_0003s0560 30.078 256 161 6 36 289 79 318 8.04e-32 125
MS.gene31224.t1 MTR_2g009200 24.788 472 254 19 16 443 21 435 4.20e-29 119
MS.gene31224.t1 MTR_4g056520 24.883 426 266 12 57 443 44 454 2.41e-27 114
MS.gene31224.t1 MTR_2g084530 27.553 421 249 16 57 438 44 447 1.08e-25 109
MS.gene31224.t1 MTR_2g018630 25.205 365 229 13 106 445 13 358 9.87e-24 102
MS.gene31224.t1 MTR_1g102130 23.611 432 247 17 46 445 107 487 2.25e-20 94.0
MS.gene31224.t1 MTR_4g021535 24.163 418 216 17 58 442 65 414 4.63e-19 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31224.t1 AT3G45010 65.899 434 136 5 22 448 74 502 0.0 605
MS.gene31224.t1 AT5G22980 67.633 414 129 3 36 449 90 498 0.0 591
MS.gene31224.t1 AT3G10410 63.850 426 148 4 22 446 76 496 0.0 588
MS.gene31224.t1 AT5G22960 60.714 168 45 3 38 205 28 174 1.50e-64 206
MS.gene31224.t1 AT2G22920 30.180 444 266 18 16 444 18 432 2.22e-53 185
MS.gene31224.t1 AT1G15000 31.159 414 257 11 40 445 31 424 3.99e-53 184
MS.gene31224.t1 AT2G22970 29.213 445 269 18 16 445 18 431 8.24e-53 183
MS.gene31224.t1 AT1G73300 29.412 442 272 16 16 445 26 439 8.69e-51 178
MS.gene31224.t1 AT1G73300 29.412 442 272 16 16 445 26 439 8.69e-51 178
MS.gene31224.t1 AT3G12203 26.747 415 264 13 46 445 25 414 1.10e-49 175
MS.gene31224.t1 AT2G22920 31.771 384 225 15 16 385 18 378 1.20e-49 174
MS.gene31224.t1 AT2G22970 31.250 384 225 15 16 385 18 376 1.29e-49 174
MS.gene31224.t1 AT4G12910 28.270 474 265 15 29 444 38 494 1.83e-49 176
MS.gene31224.t1 AT3G12203 26.747 415 264 13 46 445 46 435 2.05e-49 174
MS.gene31224.t1 AT3G25420 28.542 480 260 17 29 445 33 492 2.46e-49 176
MS.gene31224.t1 AT2G22970 31.250 384 225 15 16 385 18 376 3.34e-49 174
MS.gene31224.t1 AT3G12203 27.114 402 253 13 59 445 1 377 4.37e-49 172
MS.gene31224.t1 AT5G36180 28.507 442 276 15 16 445 26 439 1.96e-48 172
MS.gene31224.t1 AT2G23010 28.731 449 270 18 16 445 18 435 2.31e-48 172
MS.gene31224.t1 AT2G23010 28.731 449 270 18 16 445 18 435 5.56e-48 171
MS.gene31224.t1 AT1G73290 27.314 443 278 15 16 445 25 436 7.74e-48 170
MS.gene31224.t1 AT1G73270 26.862 443 282 15 16 445 26 439 1.01e-47 170
MS.gene31224.t1 AT1G73270 26.862 443 282 15 16 445 26 439 1.21e-47 170
MS.gene31224.t1 AT2G22980 28.959 442 270 18 16 445 19 428 1.52e-47 169
MS.gene31224.t1 AT1G73280 27.376 442 281 14 16 445 26 439 2.04e-47 169
MS.gene31224.t1 AT3G12240 28.846 416 255 16 46 445 44 434 2.34e-47 169
MS.gene31224.t1 AT3G10450 28.140 430 272 14 28 445 31 435 2.87e-47 169
MS.gene31224.t1 AT3G10450 27.561 410 264 12 48 445 4 392 3.73e-46 165
MS.gene31224.t1 AT1G73290 29.058 382 234 12 16 385 25 381 3.92e-46 165
MS.gene31224.t1 AT3G12230 28.365 416 257 15 46 445 43 433 1.26e-45 164
MS.gene31224.t1 AT4G30810 26.218 431 268 12 42 444 50 458 3.96e-45 164
MS.gene31224.t1 AT2G22990 27.938 451 269 18 16 445 16 431 5.11e-45 162
MS.gene31224.t1 AT3G12220 26.316 418 267 14 45 446 42 434 6.01e-45 162
MS.gene31224.t1 AT4G12910 31.940 335 199 11 29 352 38 354 6.16e-45 163
MS.gene31224.t1 AT1G73310 27.703 444 277 16 16 445 26 439 1.34e-44 162
MS.gene31224.t1 AT2G22980 28.671 429 263 17 16 432 19 416 1.84e-44 161
MS.gene31224.t1 AT3G12220 26.667 405 256 14 58 446 25 404 2.02e-44 160
MS.gene31224.t1 AT2G22980 29.117 419 254 17 16 422 19 406 2.17e-44 160
MS.gene31224.t1 AT3G17180 27.765 443 262 15 41 445 50 472 2.18e-44 162
MS.gene31224.t1 AT2G22920 34.395 314 179 12 16 321 18 312 3.33e-44 157
MS.gene31224.t1 AT4G15100 26.959 434 270 15 44 444 58 477 4.45e-44 161
MS.gene31224.t1 AT2G23000 27.553 421 258 15 46 445 41 435 7.03e-44 160
MS.gene31224.t1 AT2G23000 27.553 421 258 15 46 445 41 435 7.03e-44 160
MS.gene31224.t1 AT5G23210 28.281 442 263 14 44 445 65 492 1.14e-43 160
MS.gene31224.t1 AT5G08260 29.425 435 254 17 44 442 53 470 1.21e-43 160
MS.gene31224.t1 AT1G28110 26.824 425 272 13 44 444 45 454 1.33e-43 159
MS.gene31224.t1 AT1G28110 26.824 425 272 13 44 444 45 454 1.33e-43 159
MS.gene31224.t1 AT1G61130 28.169 426 267 14 44 444 45 456 1.85e-42 156
MS.gene31224.t1 AT3G10450 27.764 407 258 13 28 422 31 413 2.01e-42 155
MS.gene31224.t1 AT2G22970 33.438 317 183 12 16 324 18 314 2.12e-42 153
MS.gene31224.t1 AT3G52000 28.117 409 273 12 44 443 78 474 3.64e-42 156
MS.gene31224.t1 AT3G63470 28.979 421 245 16 44 438 95 487 4.01e-42 156
MS.gene31224.t1 AT2G33530 27.273 429 269 13 44 445 47 459 4.64e-42 155
MS.gene31224.t1 AT1G11080 27.356 435 269 15 44 444 64 485 5.97e-42 155
MS.gene31224.t1 AT1G11080 27.356 435 269 15 44 444 64 485 6.99e-42 155
MS.gene31224.t1 AT1G73290 31.849 292 170 9 16 300 25 294 7.76e-42 151
MS.gene31224.t1 AT1G33540 25.747 435 271 14 46 445 45 462 1.21e-41 154
MS.gene31224.t1 AT2G35770 28.818 406 259 11 46 435 54 445 1.44e-41 154
MS.gene31224.t1 AT4G30610 26.620 432 273 14 42 445 44 459 2.02e-41 154
MS.gene31224.t1 AT2G22990 28.009 432 254 17 16 424 16 413 3.20e-41 152
MS.gene31224.t1 AT3G10450 27.132 387 250 11 48 422 4 370 3.21e-41 151
MS.gene31224.t1 AT3G07990 27.918 437 256 16 41 445 45 454 3.35e-41 153
MS.gene31224.t1 AT1G73310 28.982 383 235 13 16 385 26 384 4.09e-41 152
MS.gene31224.t1 AT1G33540 25.635 433 270 14 48 445 4 419 4.18e-41 152
MS.gene31224.t1 AT2G22990 27.804 428 254 17 16 422 16 409 7.41e-41 152
MS.gene31224.t1 AT3G52020 26.698 427 260 14 44 443 93 493 8.86e-41 152
MS.gene31224.t1 AT5G23210 39.706 204 118 5 44 243 65 267 1.50e-40 151
MS.gene31224.t1 AT3G52010 26.852 432 279 14 29 443 68 479 1.85e-40 151
MS.gene31224.t1 AT2G24010 28.046 435 275 13 29 445 34 448 2.69e-40 150
MS.gene31224.t1 AT2G22980 32.911 316 178 12 16 321 19 310 5.48e-39 144
MS.gene31224.t1 AT2G35780 26.651 424 274 13 42 445 42 448 7.43e-39 146
MS.gene31224.t1 AT1G73280 30.892 314 190 9 16 321 26 320 1.38e-38 144
MS.gene31224.t1 AT2G24000 27.149 442 278 17 29 444 34 457 1.88e-38 145
MS.gene31224.t1 AT1G43780 27.594 424 274 16 46 445 53 467 2.07e-38 145
MS.gene31224.t1 AT2G22990 26.608 451 266 19 16 445 16 422 5.59e-38 143
MS.gene31224.t1 AT2G12480 27.294 425 272 15 29 445 35 430 3.83e-37 141
MS.gene31224.t1 AT5G09640 25.482 467 289 18 16 445 19 463 4.09e-37 142
MS.gene31224.t1 AT2G12480 26.351 444 276 16 29 445 35 454 1.26e-36 140
MS.gene31224.t1 AT5G42230 26.118 425 282 14 44 445 42 457 1.67e-36 140
MS.gene31224.t1 AT5G42230 26.118 425 282 14 44 445 42 457 1.67e-36 140
MS.gene31224.t1 AT3G17180 33.061 245 144 8 41 277 50 282 3.58e-36 139
MS.gene31224.t1 AT2G12480 27.529 425 269 16 29 445 35 428 4.20e-36 139
MS.gene31224.t1 AT3G12220 33.981 206 130 3 45 245 42 246 9.75e-36 136
MS.gene31224.t1 AT2G24010 26.444 450 278 13 29 445 34 463 1.46e-35 137
MS.gene31224.t1 AT2G05850 25.442 452 292 16 12 443 53 479 4.06e-35 136
MS.gene31224.t1 AT2G12480 27.465 426 269 16 29 445 35 429 5.94e-35 135
MS.gene31224.t1 AT5G42240 25.463 432 276 14 44 445 46 461 6.17e-35 136
MS.gene31224.t1 AT3G02110 24.884 430 279 11 46 445 53 468 1.27e-34 135
MS.gene31224.t1 AT5G23210 27.475 404 240 13 81 445 8 397 1.88e-33 130
MS.gene31224.t1 AT5G23210 27.295 403 240 13 82 445 8 396 9.69e-33 128
MS.gene31224.t1 AT2G24010 25.745 470 281 16 29 445 5 459 2.05e-31 125
MS.gene31224.t1 AT5G36180 26.225 347 228 10 106 445 12 337 7.41e-31 122
MS.gene31224.t1 AT5G36180 26.225 347 228 10 106 445 12 337 7.41e-31 122
MS.gene31224.t1 AT1G73280 25.072 347 232 9 106 445 12 337 1.91e-30 120
MS.gene31224.t1 AT2G27920 26.118 425 254 14 57 438 45 452 5.53e-30 122
MS.gene31224.t1 AT5G23210 40.000 165 95 4 82 243 8 171 7.22e-30 119
MS.gene31224.t1 AT3G56540 39.437 142 82 4 44 183 93 232 2.74e-28 113
MS.gene31224.t1 AT2G22990 25.000 344 214 12 118 445 2 317 2.82e-28 114
MS.gene31224.t1 AT2G22990 25.000 344 214 12 118 445 2 317 9.64e-28 113
MS.gene31224.t1 AT2G24010 30.855 269 151 10 29 277 5 258 1.06e-26 110
MS.gene31224.t1 AT5G36180 27.273 286 187 7 106 385 12 282 1.34e-26 109
MS.gene31224.t1 AT5G36180 27.273 286 187 7 106 385 12 282 1.67e-26 110
MS.gene31224.t1 AT1G73280 23.724 333 226 9 120 445 1 312 1.72e-25 106
MS.gene31224.t1 AT2G22980 25.962 312 200 11 118 422 2 289 2.54e-24 102
MS.gene31224.t1 AT1G73310 25.694 288 189 7 106 385 12 282 2.56e-24 103
MS.gene31224.t1 AT2G27920 25.253 396 240 13 80 438 4 380 2.30e-23 102
MS.gene31224.t1 AT2G27920 23.851 348 219 10 127 438 48 385 3.24e-17 83.6
MS.gene31224.t1 AT2G22920 21.691 272 182 10 183 444 3 253 3.35e-12 67.0

Find 108 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATTCTCTGGTATGTTTA+TGG 0.137565 2.4:-7056635 None:intergenic
ACAACATGGCATCATCTTTA+AGG 0.142828 2.4:+7056483 None:intergenic
ATACAACAAGAAAGTGATTT+TGG 0.209981 2.4:-7058964 None:intergenic
TATGCCATGTACTGAATTTC+AGG 0.244869 2.4:-7057818 None:intergenic
TCCCTTGTTTGGAATGAATA+TGG 0.250663 2.4:+7057021 MS.gene31224:CDS
GTCAGGTCAGACAACATTTA+AGG 0.308088 2.4:+7058637 MS.gene31224:CDS
CACTTCAAATGTTACAAGAT+TGG 0.313206 2.4:+7058912 MS.gene31224:CDS
ACAAGAACTTGTCCCTTGTT+TGG 0.329032 2.4:+7057010 MS.gene31224:CDS
AATAAACAGGGCTTTGCTAT+TGG 0.339075 2.4:+7057779 MS.gene31224:intron
TGGACCTTTGGCTTTCCTTA+AGG 0.353120 2.4:+7058718 MS.gene31224:CDS
AACCATTTACCTAGCAGCTT+TGG 0.353226 2.4:-7056776 None:intergenic
CGATTTGTATGACTTCTTGC+AGG 0.353476 2.4:+7057253 MS.gene31224:CDS
CAGGGCTTTGCTATTGGTAA+TGG 0.354719 2.4:+7057785 MS.gene31224:intron
CTCATTTGGTAGGGAATTCA+AGG 0.357235 2.4:+7058594 MS.gene31224:intron
ACAGCATACGTTAGTGTTGC+CGG 0.368221 2.4:-7058140 None:intergenic
AGGCAATGGACTGATTAATA+AGG 0.371408 2.4:+7057856 MS.gene31224:CDS
TAAAGGAAGCTTTAGGTGTT+GGG 0.376899 2.4:+7058346 MS.gene31224:CDS
CCTGTCGTCATATGGTTAAC+TGG 0.391322 2.4:+7056922 MS.gene31224:CDS
GGTTATTCTGTTGAAGAACT+TGG 0.406042 2.4:+7056721 MS.gene31224:CDS
TGTCTGACCTGACCACTTCA+TGG 0.416219 2.4:-7058627 None:intergenic
CTTAGTACACGCTGCCTTGA+CGG 0.424079 2.4:+7058395 MS.gene31224:CDS
AAGAAATGTGAGGGAGCTTT+CGG 0.426372 2.4:+7058273 MS.gene31224:CDS
TTGTCCTTTGAGAACTGAAA+AGG 0.434846 2.4:-7056991 None:intergenic
GGGAGCTTTCGGTGATTGTC+AGG 0.436170 2.4:+7058284 MS.gene31224:CDS
CGAACCCTGGATGCAAAAGC+TGG 0.440348 2.4:-7057593 None:intergenic
GGCTGGTCACATGGTTCCTA+TGG 0.441603 2.4:+7058874 MS.gene31224:CDS
GTAGACCAACCCATTGGATC+AGG 0.443437 2.4:+7057170 MS.gene31224:CDS
AACATTTGAAGTGCAACTTT+TGG 0.446825 2.4:-7058902 None:intergenic
GAGAAGAAGTTCACTTTCCC+TGG 0.449586 2.4:+7056685 MS.gene31224:CDS
CTTTGAATCTCGAAACAGTA+AGG 0.449789 2.4:+7056894 MS.gene31224:CDS
ACGCTGCCTTGACGGAAGAT+TGG 0.455836 2.4:+7058403 MS.gene31224:CDS
AAGACAGTAAAGGAAGCTTT+AGG 0.467806 2.4:+7058339 MS.gene31224:CDS
GCAATTTCACTGCCGCATCC+TGG 0.467824 2.4:-7056949 None:intergenic
TTTGTTTCCTTGGCGAACCC+TGG 0.473602 2.4:-7057606 None:intergenic
GCATATACAGATTATGCATT+AGG 0.474872 2.4:+7057836 MS.gene31224:CDS
ATTCCTGCTCTTCTTGAGGA+TGG 0.475493 2.4:+7058447 MS.gene31224:CDS
GAAGAACTTGGTCATCATGC+AGG 0.475724 2.4:+7056733 MS.gene31224:CDS
TTTGGAACAATAGGTGAAAG+AGG 0.477010 2.4:+7058853 MS.gene31224:intron
CATGTACTGAATTTCAGGAT+TGG 0.477634 2.4:-7057813 None:intergenic
GTAAAGGAAGCTTTAGGTGT+TGG 0.480724 2.4:+7058345 MS.gene31224:CDS
TTGGTTGATAGTGAAGAAGC+AGG 0.485779 2.4:+7058680 MS.gene31224:CDS
AGGAATTCATATAAATCTAA+AGG 0.501724 2.4:+7057637 MS.gene31224:CDS
TCTTCTCCACAATGTTTCCA+TGG 0.501980 2.4:-7056669 None:intergenic
TACCTAGCAGCTTTGGAATG+AGG 0.502116 2.4:-7056769 None:intergenic
CAATCCTGAAATTCAGTACA+TGG 0.506627 2.4:+7057814 MS.gene31224:CDS
GGGTATTCCTGCTCTTCTTG+AGG 0.508272 2.4:+7058443 MS.gene31224:CDS
ATTTATCTGTTAGGCACTGA+AGG 0.510266 2.4:+7058052 MS.gene31224:intron
GTTGATCCCACCGTGTCTAA+AGG 0.511902 2.4:+7057901 MS.gene31224:CDS
AATCATTCTTTGTGAACTGA+GGG 0.514863 2.4:-7057532 None:intergenic
CGCCGTGATGAAGACAGTAT+TGG 0.518941 2.4:+7057227 MS.gene31224:CDS
CCAGTTAACCATATGACGAC+AGG 0.521891 2.4:-7056922 None:intergenic
ATGTCCAGCGTATGACTCTC+CGG 0.523072 2.4:-7057564 None:intergenic
ATTCCATCCTCAAGAAGAGC+AGG 0.526347 2.4:-7058450 None:intergenic
AGGATGACCCTGTCGTCATA+TGG 0.526809 2.4:+7056914 MS.gene31224:CDS
AGGACATGAGGATCATTCTC+TGG 0.531938 2.4:-7056646 None:intergenic
GAACAATAGGTGAAAGAGGC+TGG 0.533723 2.4:+7058857 MS.gene31224:intron
GATCTAATATGCAATTGGCT+TGG 0.534093 2.4:+7058501 MS.gene31224:CDS
CAAGGAAACAAAGCAAAAGA+AGG 0.534129 2.4:+7057617 MS.gene31224:CDS
ATATGGTTAACTGGAGGTCC+AGG 0.542845 2.4:+7056931 MS.gene31224:CDS
GTTGATGCCATGAAGTGGTC+AGG 0.543073 2.4:+7058620 MS.gene31224:CDS
CTTGAACATGAAGACAGTAA+AGG 0.545435 2.4:+7058329 MS.gene31224:CDS
ATGTTACAAGATTGGATGCA+AGG 0.545582 2.4:+7058920 MS.gene31224:CDS
AAATCATTCTTTGTGAACTG+AGG 0.546581 2.4:-7057533 None:intergenic
ATATTTGTAGACCAACCCAT+TGG 0.549517 2.4:+7057164 MS.gene31224:CDS
TTGTTTGGAATGAATATGGA+TGG 0.552020 2.4:+7057025 MS.gene31224:CDS
ATAGTTGAAACCTGATCCAA+TGG 0.554532 2.4:-7057180 None:intergenic
TGTTACAAGATTGGATGCAA+GGG 0.561679 2.4:+7058921 MS.gene31224:CDS
CTGCCGCCTTTAGACACGGT+GGG 0.565722 2.4:-7057907 None:intergenic
AAAGGAAGCTTTAGGTGTTG+GGG 0.573810 2.4:+7058347 MS.gene31224:CDS
AATGATCCTCATGTCCTCCA+TGG 0.578894 2.4:+7056652 MS.gene31224:CDS
ACAGATTATGCATTAGGCAA+TGG 0.580565 2.4:+7057842 MS.gene31224:CDS
TTAACTGGAGGTCCAGGATG+CGG 0.584480 2.4:+7056937 MS.gene31224:CDS
GACATTAGAAAGAAATGTGA+GGG 0.586086 2.4:+7058264 MS.gene31224:CDS
GGTGAAAGAGGCTGGTCACA+TGG 0.589518 2.4:+7058865 MS.gene31224:intron
TTTGGTAGGGAATTCAAGGT+GGG 0.590648 2.4:+7058598 MS.gene31224:intron
ACTGAAGGTGGACAAGCATG+TGG 0.599485 2.4:+7058067 MS.gene31224:CDS
ATCCATATTCATTCCAAACA+AGG 0.604369 2.4:-7057023 None:intergenic
TTCCTCATTCCAAAGCTGCT+AGG 0.605027 2.4:+7056767 MS.gene31224:CDS
TAACCAGAGTCATCAAAACC+AGG 0.605492 2.4:-7056703 None:intergenic
TTTCATCCAATCTTCCGTCA+AGG 0.611684 2.4:-7058409 None:intergenic
TTGTCAGGACTTCTCTGACA+TGG 0.615587 2.4:+7058299 MS.gene31224:CDS
TAGTTGAAACCTGATCCAAT+GGG 0.617618 2.4:-7057179 None:intergenic
TGAAACCTGATCCAATGGGT+TGG 0.626101 2.4:-7057175 None:intergenic
GCGAATATCACTGTGAACAT+CGG 0.629348 2.4:-7057207 None:intergenic
ATGTTTCCATGGAGGACATG+AGG 0.630827 2.4:-7056658 None:intergenic
TATCTGTTAGGCACTGAAGG+TGG 0.631703 2.4:+7058055 MS.gene31224:intron
ATTTGGTAGGGAATTCAAGG+TGG 0.632416 2.4:+7058597 MS.gene31224:intron
AAGAACTTGGTCATCATGCA+GGG 0.634335 2.4:+7056734 MS.gene31224:CDS
TGTCCTCCATGGAAACATTG+TGG 0.634451 2.4:+7056663 MS.gene31224:CDS
GATCAGATCACAAACATCAC+CGG 0.635185 2.4:+7058121 MS.gene31224:CDS
TCCATATTCATTCCAAACAA+GGG 0.638155 2.4:-7057022 None:intergenic
GAATGAATATGGATGGGACA+AGG 0.638592 2.4:+7057032 MS.gene31224:CDS
TTTGCATCCAGGGTTCGCCA+AGG 0.639567 2.4:+7057599 MS.gene31224:CDS
CGACATTAGAAAGAAATGTG+AGG 0.647229 2.4:+7058263 MS.gene31224:CDS
AACCAGAGTCATCAAAACCA+GGG 0.649229 2.4:-7056702 None:intergenic
GTCGTCATATGGTTAACTGG+AGG 0.656929 2.4:+7056925 MS.gene31224:CDS
ATCAAGTTACTTGTGTACGC+CGG 0.657584 2.4:+7058471 MS.gene31224:CDS
CAGTTAACCATATGACGACA+GGG 0.657833 2.4:-7056921 None:intergenic
TGCCAATACTGTCTTCATCA+CGG 0.659820 2.4:-7057229 None:intergenic
GATCCCACCGTGTCTAAAGG+CGG 0.662660 2.4:+7057904 MS.gene31224:CDS
CTGAAGGTGGACAAGCATGT+GGG 0.668943 2.4:+7058068 MS.gene31224:CDS
TGTTTGGAATGAATATGGAT+GGG 0.672561 2.4:+7057026 MS.gene31224:CDS
TCTCCACAATGTTTCCATGG+AGG 0.678885 2.4:-7056666 None:intergenic
TCTGCCGCCTTTAGACACGG+TGG 0.679091 2.4:-7057908 None:intergenic
ATTACCGGAGAGTCATACGC+TGG 0.680484 2.4:+7057560 MS.gene31224:CDS
GGTGGGTTGATGCCATGAAG+TGG 0.684602 2.4:+7058615 MS.gene31224:CDS
AATATCACTGTGAACATCGG+TGG 0.689654 2.4:-7057204 None:intergenic
CAAACCTTAAGGAAAGCCAA+AGG 0.725444 2.4:-7058722 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTTTATTATAAAAGATGAA+AGG - chr2.4:7057767-7057786 None:intergenic 15.0%
!!! AAGAATGATTTTTATATTAC+CGG + chr2.4:7057545-7057564 MS.gene31224:CDS 15.0%
!!! ACTTAGCTATATATATTTTT+TGG + chr2.4:7058835-7058854 MS.gene31224:intron 15.0%
!!! TATATATTTTTTGGAACAAT+AGG + chr2.4:7058844-7058863 MS.gene31224:intron 15.0%
!!! TTCATCTTTTATAATAAACA+GGG + chr2.4:7057767-7057786 MS.gene31224:intron 15.0%
!!! TTTCATCTTTTATAATAAAC+AGG + chr2.4:7057766-7057785 MS.gene31224:intron 15.0%
!! AAAAAGATCTAATATGCAAT+TGG + chr2.4:7058496-7058515 MS.gene31224:CDS 20.0%
!! AGGAATTCATATAAATCTAA+AGG + chr2.4:7057637-7057656 MS.gene31224:CDS 20.0%
!! ATAACTGTATATTCATACAA+AGG + chr2.4:7057691-7057710 MS.gene31224:intron 20.0%
!! ATTAAAGTGGAAAAAAGAAA+AGG - chr2.4:7057077-7057096 None:intergenic 20.0%
!! TTAAAGTGGAAAAAAGAAAA+GGG - chr2.4:7057076-7057095 None:intergenic 20.0%
!!! ACTTAAATTTTTACAATGAC+AGG + chr2.4:7057493-7057512 MS.gene31224:intron 20.0%
!!! ATAAATTAAGAGTGTCAATA+AGG - chr2.4:7058038-7058057 None:intergenic 20.0%
!!! ATTTGTATCTTGTTTAAGAT+TGG - chr2.4:7058545-7058564 None:intergenic 20.0%
!!! TTTTTTTACGAGAAATTAAG+TGG + chr2.4:7057341-7057360 MS.gene31224:intron 20.0%
! AAATTGGTAAAATTCTCTCT+TGG - chr2.4:7057990-7058009 None:intergenic 25.0%
! ACACTCTTAATTTATCTGTT+AGG + chr2.4:7058043-7058062 MS.gene31224:intron 25.0%
! GATTAATCACTTGAATTAAG+TGG + chr2.4:7057306-7057325 MS.gene31224:intron 25.0%
! TGACATAACAAATTGTTCAA+TGG - chr2.4:7056852-7056871 None:intergenic 25.0%
!! AAAGTAGCACTAAAAGTATA+AGG + chr2.4:7058172-7058191 MS.gene31224:intron 25.0%
!! ATACAACAAGAAAGTGATTT+TGG - chr2.4:7058967-7058986 None:intergenic 25.0%
!!! ATTGCCTTGTTTTATGAAAA+TGG + chr2.4:7056967-7056986 MS.gene31224:CDS 25.0%
!!! CTTTTTTCTTTCTCTTTTGT+AGG + chr2.4:7057130-7057149 MS.gene31224:intron 25.0%
!!! TTATGGAACTTTTAGGAAAA+AGG - chr2.4:7056621-7056640 None:intergenic 25.0%
AAATCATTCTTTGTGAACTG+AGG - chr2.4:7057536-7057555 None:intergenic 30.0%
AATCATTCTTTGTGAACTGA+GGG - chr2.4:7057535-7057554 None:intergenic 30.0%
ATCATTCTCTGGTATGTTTA+TGG - chr2.4:7056638-7056657 None:intergenic 30.0%
ATCCATATTCATTCCAAACA+AGG - chr2.4:7057026-7057045 None:intergenic 30.0%
ATCCTTAAACACAAACCTTA+AGG - chr2.4:7058736-7058755 None:intergenic 30.0%
CACTTCAAATGTTACAAGAT+TGG + chr2.4:7058912-7058931 MS.gene31224:CDS 30.0%
GACATTAGAAAGAAATGTGA+GGG + chr2.4:7058264-7058283 MS.gene31224:CDS 30.0%
GCATATACAGATTATGCATT+AGG + chr2.4:7057836-7057855 MS.gene31224:CDS 30.0%
TCAACCAAAAATGAAGTTGT+AGG - chr2.4:7058668-7058687 None:intergenic 30.0%
TCCATATTCATTCCAAACAA+GGG - chr2.4:7057025-7057044 None:intergenic 30.0%
TGGATGAAAAATCTTGAAGT+GGG + chr2.4:7058423-7058442 MS.gene31224:CDS 30.0%
TGTATCAGTGAGAATTAAAG+TGG - chr2.4:7057090-7057109 None:intergenic 30.0%
TTCCTTAAGGTTTGTGTTTA+AGG + chr2.4:7058731-7058750 MS.gene31224:intron 30.0%
TTGGATGAAAAATCTTGAAG+TGG + chr2.4:7058422-7058441 MS.gene31224:CDS 30.0%
TTGTAAAAGACAGACACATT+TGG - chr2.4:7056514-7056533 None:intergenic 30.0%
! ACATGACTTGCACAATAAAT+TGG - chr2.4:7058006-7058025 None:intergenic 30.0%
! AGGTCCATTTTCATAAAACA+AGG - chr2.4:7056974-7056993 None:intergenic 30.0%
! CTTCAAATTAAAAGTGTGTC+TGG + chr2.4:7058800-7058819 MS.gene31224:intron 30.0%
! GCATATTAGATCTTTTTCTC+CGG - chr2.4:7058493-7058512 None:intergenic 30.0%
! GGTATGTTTATGGAACTTTT+AGG - chr2.4:7056628-7056647 None:intergenic 30.0%
! TGTTTGGAATGAATATGGAT+GGG + chr2.4:7057026-7057045 MS.gene31224:CDS 30.0%
! TTGTTTGGAATGAATATGGA+TGG + chr2.4:7057025-7057044 MS.gene31224:CDS 30.0%
!! AACATTTGAAGTGCAACTTT+TGG - chr2.4:7058905-7058924 None:intergenic 30.0%
!! CAATTTGTTATGTCAATTGC+AGG + chr2.4:7056857-7056876 MS.gene31224:intron 30.0%
AAATGTGCTTCTCATTTGGT+AGG + chr2.4:7058584-7058603 MS.gene31224:intron 35.0%
AAGACAGTAAAGGAAGCTTT+AGG + chr2.4:7058339-7058358 MS.gene31224:CDS 35.0%
AATGTGCTTCTCATTTGGTA+GGG + chr2.4:7058585-7058604 MS.gene31224:intron 35.0%
ACAGATTATGCATTAGGCAA+TGG + chr2.4:7057842-7057861 MS.gene31224:CDS 35.0%
ACATCATAATAACGTGTGCA+TGG + chr2.4:7056818-7056837 MS.gene31224:intron 35.0%
ACGACACTACATACTATCTA+TGG + chr2.4:7057417-7057436 MS.gene31224:intron 35.0%
AGTTGAAGAAAGGAGAATGA+AGG - chr2.4:7056566-7056585 None:intergenic 35.0%
ATAGTTGAAACCTGATCCAA+TGG - chr2.4:7057183-7057202 None:intergenic 35.0%
ATATTTGTAGACCAACCCAT+TGG + chr2.4:7057164-7057183 MS.gene31224:CDS 35.0%
ATGAAGGTGAAATGAAGAAG+AGG - chr2.4:7056550-7056569 None:intergenic 35.0%
ATGCACACGTTATTATGATG+TGG - chr2.4:7056819-7056838 None:intergenic 35.0%
ATGTTACAAGATTGGATGCA+AGG + chr2.4:7058920-7058939 MS.gene31224:CDS 35.0%
CAAGGAAACAAAGCAAAAGA+AGG + chr2.4:7057617-7057636 MS.gene31224:CDS 35.0%
CAATCCTGAAATTCAGTACA+TGG + chr2.4:7057814-7057833 MS.gene31224:CDS 35.0%
CATGTACTGAATTTCAGGAT+TGG - chr2.4:7057816-7057835 None:intergenic 35.0%
CGACATTAGAAAGAAATGTG+AGG + chr2.4:7058263-7058282 MS.gene31224:CDS 35.0%
CTCTAAATGTGCTTCTCATT+TGG + chr2.4:7058580-7058599 MS.gene31224:intron 35.0%
CTTGAACATGAAGACAGTAA+AGG + chr2.4:7058329-7058348 MS.gene31224:CDS 35.0%
CTTTGAATCTCGAAACAGTA+AGG + chr2.4:7056894-7056913 MS.gene31224:CDS 35.0%
GATCTAATATGCAATTGGCT+TGG + chr2.4:7058501-7058520 MS.gene31224:CDS 35.0%
GGTTATTCTGTTGAAGAACT+TGG + chr2.4:7056721-7056740 MS.gene31224:CDS 35.0%
TAAAAGACAGACACATTTGG+AGG - chr2.4:7056511-7056530 None:intergenic 35.0%
TAGTTGAAACCTGATCCAAT+GGG - chr2.4:7057182-7057201 None:intergenic 35.0%
TATGCCATGTACTGAATTTC+AGG - chr2.4:7057821-7057840 None:intergenic 35.0%
TCTGAAAAATCATGGACCTT+TGG + chr2.4:7058706-7058725 MS.gene31224:CDS 35.0%
TGCACACGTTATTATGATGT+GGG - chr2.4:7056818-7056837 None:intergenic 35.0%
TGTTACAAGATTGGATGCAA+GGG + chr2.4:7058921-7058940 MS.gene31224:CDS 35.0%
! AATAAACAGGGCTTTGCTAT+TGG + chr2.4:7057779-7057798 MS.gene31224:intron 35.0%
! ACAATGACAGGCATTTTTCA+AGG + chr2.4:7057505-7057524 MS.gene31224:intron 35.0%
! GCAGGAATTCTGAAAAATCA+TGG + chr2.4:7058698-7058717 MS.gene31224:CDS 35.0%
! GTCTCCTACAACTTCATTTT+TGG + chr2.4:7058661-7058680 MS.gene31224:CDS 35.0%
! TCCCTTGTTTGGAATGAATA+TGG + chr2.4:7057021-7057040 MS.gene31224:CDS 35.0%
! TTGTCCTTTGAGAACTGAAA+AGG - chr2.4:7056994-7057013 None:intergenic 35.0%
! TTTGGAACAATAGGTGAAAG+AGG + chr2.4:7058853-7058872 MS.gene31224:intron 35.0%
!! AGGCAATGGACTGATTAATA+AGG + chr2.4:7057856-7057875 MS.gene31224:CDS 35.0%
!! ATTTATCTGTTAGGCACTGA+AGG + chr2.4:7058052-7058071 MS.gene31224:intron 35.0%
!! TAAAGGAAGCTTTAGGTGTT+GGG + chr2.4:7058346-7058365 MS.gene31224:CDS 35.0%
!!! CTTCTTTTGCTTTGTTTCCT+TGG - chr2.4:7057619-7057638 None:intergenic 35.0%
AACCAGAGTCATCAAAACCA+GGG - chr2.4:7056705-7056724 None:intergenic 40.0%
AACCATTTACCTAGCAGCTT+TGG - chr2.4:7056779-7056798 None:intergenic 40.0%
AAGAAATGTGAGGGAGCTTT+CGG + chr2.4:7058273-7058292 MS.gene31224:CDS 40.0%
AAGAACTTGGTCATCATGCA+GGG + chr2.4:7056734-7056753 MS.gene31224:CDS 40.0%
AATATCACTGTGAACATCGG+TGG - chr2.4:7057207-7057226 None:intergenic 40.0%
ACAAGAACTTGTCCCTTGTT+TGG + chr2.4:7057010-7057029 MS.gene31224:CDS 40.0%
AGTCCTCTTATGAGAGTTGA+AGG - chr2.4:7056595-7056614 None:intergenic 40.0%
ATCAAGTTACTTGTGTACGC+CGG + chr2.4:7058471-7058490 MS.gene31224:CDS 40.0%
ATTTGGTAGGGAATTCAAGG+TGG + chr2.4:7058597-7058616 MS.gene31224:intron 40.0%
CAAACCTTAAGGAAAGCCAA+AGG - chr2.4:7058725-7058744 None:intergenic 40.0%
CAGTTAACCATATGACGACA+GGG - chr2.4:7056924-7056943 None:intergenic 40.0%
CGATTTGTATGACTTCTTGC+AGG + chr2.4:7057253-7057272 MS.gene31224:CDS 40.0%
CTCATTTGGTAGGGAATTCA+AGG + chr2.4:7058594-7058613 MS.gene31224:intron 40.0%
GAATGAATATGGATGGGACA+AGG + chr2.4:7057032-7057051 MS.gene31224:CDS 40.0%
GATCAGATCACAAACATCAC+CGG + chr2.4:7058121-7058140 MS.gene31224:CDS 40.0%
GCACACGTTATTATGATGTG+GGG - chr2.4:7056817-7056836 None:intergenic 40.0%
GCGAATATCACTGTGAACAT+CGG - chr2.4:7057210-7057229 None:intergenic 40.0%
GTCAGGTCAGACAACATTTA+AGG + chr2.4:7058637-7058656 MS.gene31224:CDS 40.0%
GTCCTCTTATGAGAGTTGAA+GGG - chr2.4:7056594-7056613 None:intergenic 40.0%
TAACCAGAGTCATCAAAACC+AGG - chr2.4:7056706-7056725 None:intergenic 40.0%
TCCTCTTATGAGAGTTGAAG+GGG - chr2.4:7056593-7056612 None:intergenic 40.0%
TCTTCTCCACAATGTTTCCA+TGG - chr2.4:7056672-7056691 None:intergenic 40.0%
TGCCAATACTGTCTTCATCA+CGG - chr2.4:7057232-7057251 None:intergenic 40.0%
TTCCAAAGCTGCTAGGTAAA+TGG + chr2.4:7056774-7056793 MS.gene31224:intron 40.0%
TTTCATCCAATCTTCCGTCA+AGG - chr2.4:7058412-7058431 None:intergenic 40.0%
TTTGGTAGGGAATTCAAGGT+GGG + chr2.4:7058598-7058617 MS.gene31224:intron 40.0%
! TGGACCTTTTCAGTTCTCAA+AGG + chr2.4:7056987-7057006 MS.gene31224:CDS 40.0%
!! AAAGGAAGCTTTAGGTGTTG+GGG + chr2.4:7058347-7058366 MS.gene31224:CDS 40.0%
!! GCAACTTTTGGTTGATCCAT+AGG - chr2.4:7058893-7058912 None:intergenic 40.0%
!! GTAAAGGAAGCTTTAGGTGT+TGG + chr2.4:7058345-7058364 MS.gene31224:CDS 40.0%
!! TTGGTTGATAGTGAAGAAGC+AGG + chr2.4:7058680-7058699 MS.gene31224:CDS 40.0%
AAGGGGATGCAGTTGAAGAA+AGG - chr2.4:7056576-7056595 None:intergenic 45.0%
AATGATCCTCATGTCCTCCA+TGG + chr2.4:7056652-7056671 MS.gene31224:CDS 45.0%
ACAGCATACGTTAGTGTTGC+CGG - chr2.4:7058143-7058162 None:intergenic 45.0%
AGGACATGAGGATCATTCTC+TGG - chr2.4:7056649-7056668 None:intergenic 45.0%
ATATGGTTAACTGGAGGTCC+AGG + chr2.4:7056931-7056950 MS.gene31224:CDS 45.0%
ATGTTTCCATGGAGGACATG+AGG - chr2.4:7056661-7056680 None:intergenic 45.0%
ATTCCATCCTCAAGAAGAGC+AGG - chr2.4:7058453-7058472 None:intergenic 45.0%
ATTCCTGCTCTTCTTGAGGA+TGG + chr2.4:7058447-7058466 MS.gene31224:CDS 45.0%
CCAGTTAACCATATGACGAC+AGG - chr2.4:7056925-7056944 None:intergenic 45.0%
CCTGTCGTCATATGGTTAAC+TGG + chr2.4:7056922-7056941 MS.gene31224:CDS 45.0%
CTAAAGGCGGCAGAAAAATG+TGG + chr2.4:7057917-7057936 MS.gene31224:CDS 45.0%
GAACAATAGGTGAAAGAGGC+TGG + chr2.4:7058857-7058876 MS.gene31224:intron 45.0%
GAAGAACTTGGTCATCATGC+AGG + chr2.4:7056733-7056752 MS.gene31224:CDS 45.0%
GAGAAGAAGTTCACTTTCCC+TGG + chr2.4:7056685-7056704 MS.gene31224:CDS 45.0%
GTCGTCATATGGTTAACTGG+AGG + chr2.4:7056925-7056944 MS.gene31224:CDS 45.0%
TCCCCTTCAACTCTCATAAG+AGG + chr2.4:7056589-7056608 MS.gene31224:intron 45.0%
TCTCCACAATGTTTCCATGG+AGG - chr2.4:7056669-7056688 None:intergenic 45.0%
TGAAACCTGATCCAATGGGT+TGG - chr2.4:7057178-7057197 None:intergenic 45.0%
TGTCCTCCATGGAAACATTG+TGG + chr2.4:7056663-7056682 MS.gene31224:CDS 45.0%
TTCCTCATTCCAAAGCTGCT+AGG + chr2.4:7056767-7056786 MS.gene31224:CDS 45.0%
TTGTCAGGACTTCTCTGACA+TGG + chr2.4:7058299-7058318 MS.gene31224:CDS 45.0%
! ACATTCCAGCTTTTGCATCC+AGG + chr2.4:7057588-7057607 MS.gene31224:CDS 45.0%
! CATTCCAGCTTTTGCATCCA+GGG + chr2.4:7057589-7057608 MS.gene31224:CDS 45.0%
! TTTTCTGCCGCCTTTAGACA+CGG - chr2.4:7057914-7057933 None:intergenic 45.0%
!! CAGGGCTTTGCTATTGGTAA+TGG + chr2.4:7057785-7057804 MS.gene31224:intron 45.0%
!! TACCTAGCAGCTTTGGAATG+AGG - chr2.4:7056772-7056791 None:intergenic 45.0%
!! TATCTGTTAGGCACTGAAGG+TGG + chr2.4:7058055-7058074 MS.gene31224:intron 45.0%
!! TGGACCTTTGGCTTTCCTTA+AGG + chr2.4:7058718-7058737 MS.gene31224:CDS 45.0%
!!! TTCCCTGGTTTTGATGACTC+TGG + chr2.4:7056700-7056719 MS.gene31224:CDS 45.0%
ACTGAAGGTGGACAAGCATG+TGG + chr2.4:7058067-7058086 MS.gene31224:CDS 50.0%
AGGATGACCCTGTCGTCATA+TGG + chr2.4:7056914-7056933 MS.gene31224:CDS 50.0%
ATGTCCAGCGTATGACTCTC+CGG - chr2.4:7057567-7057586 None:intergenic 50.0%
ATTACCGGAGAGTCATACGC+TGG + chr2.4:7057560-7057579 MS.gene31224:CDS 50.0%
CGCCGTGATGAAGACAGTAT+TGG + chr2.4:7057227-7057246 MS.gene31224:CDS 50.0%
CTGAAGGTGGACAAGCATGT+GGG + chr2.4:7058068-7058087 MS.gene31224:CDS 50.0%
CTTAGTACACGCTGCCTTGA+CGG + chr2.4:7058395-7058414 MS.gene31224:CDS 50.0%
GGGTATTCCTGCTCTTCTTG+AGG + chr2.4:7058443-7058462 MS.gene31224:CDS 50.0%
GTAGACCAACCCATTGGATC+AGG + chr2.4:7057170-7057189 MS.gene31224:CDS 50.0%
GTTGATCCCACCGTGTCTAA+AGG + chr2.4:7057901-7057920 MS.gene31224:CDS 50.0%
GTTGATGCCATGAAGTGGTC+AGG + chr2.4:7058620-7058639 MS.gene31224:CDS 50.0%
TGTCTGACCTGACCACTTCA+TGG - chr2.4:7058630-7058649 None:intergenic 50.0%
TTAACTGGAGGTCCAGGATG+CGG + chr2.4:7056937-7056956 MS.gene31224:CDS 50.0%
TTTGTTTCCTTGGCGAACCC+TGG - chr2.4:7057609-7057628 None:intergenic 50.0%
ACGCTGCCTTGACGGAAGAT+TGG + chr2.4:7058403-7058422 MS.gene31224:CDS 55.0%
CGAACCCTGGATGCAAAAGC+TGG - chr2.4:7057596-7057615 None:intergenic 55.0%
GATCCCACCGTGTCTAAAGG+CGG + chr2.4:7057904-7057923 MS.gene31224:CDS 55.0%
GCAATTTCACTGCCGCATCC+TGG - chr2.4:7056952-7056971 None:intergenic 55.0%
GGGAGCTTTCGGTGATTGTC+AGG + chr2.4:7058284-7058303 MS.gene31224:CDS 55.0%
TTTGCATCCAGGGTTCGCCA+AGG + chr2.4:7057599-7057618 MS.gene31224:CDS 55.0%
! GGCTGGTCACATGGTTCCTA+TGG + chr2.4:7058874-7058893 MS.gene31224:CDS 55.0%
! GGTGAAAGAGGCTGGTCACA+TGG + chr2.4:7058865-7058884 MS.gene31224:intron 55.0%
!! GGTGGGTTGATGCCATGAAG+TGG + chr2.4:7058615-7058634 MS.gene31224:CDS 55.0%
CTGCCGCCTTTAGACACGGT+GGG - chr2.4:7057910-7057929 None:intergenic 60.0%
TCTGCCGCCTTTAGACACGG+TGG - chr2.4:7057911-7057930 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 7056488 7058988 7056488 ID=MS.gene31224
chr2.4 mRNA 7056488 7058988 7056488 ID=MS.gene31224.t1;Parent=MS.gene31224
chr2.4 exon 7056488 7056504 7056488 ID=MS.gene31224.t1.exon1;Parent=MS.gene31224.t1
chr2.4 CDS 7056488 7056504 7056488 ID=cds.MS.gene31224.t1;Parent=MS.gene31224.t1
chr2.4 exon 7056633 7056788 7056633 ID=MS.gene31224.t1.exon2;Parent=MS.gene31224.t1
chr2.4 CDS 7056633 7056788 7056633 ID=cds.MS.gene31224.t1;Parent=MS.gene31224.t1
chr2.4 exon 7056879 7057053 7056879 ID=MS.gene31224.t1.exon3;Parent=MS.gene31224.t1
chr2.4 CDS 7056879 7057053 7056879 ID=cds.MS.gene31224.t1;Parent=MS.gene31224.t1
chr2.4 exon 7057152 7057274 7057152 ID=MS.gene31224.t1.exon4;Parent=MS.gene31224.t1
chr2.4 CDS 7057152 7057274 7057152 ID=cds.MS.gene31224.t1;Parent=MS.gene31224.t1
chr2.4 exon 7057515 7057658 7057515 ID=MS.gene31224.t1.exon5;Parent=MS.gene31224.t1
chr2.4 CDS 7057515 7057658 7057515 ID=cds.MS.gene31224.t1;Parent=MS.gene31224.t1
chr2.4 exon 7057788 7057938 7057788 ID=MS.gene31224.t1.exon6;Parent=MS.gene31224.t1
chr2.4 CDS 7057788 7057938 7057788 ID=cds.MS.gene31224.t1;Parent=MS.gene31224.t1
chr2.4 exon 7058065 7058153 7058065 ID=MS.gene31224.t1.exon7;Parent=MS.gene31224.t1
chr2.4 CDS 7058065 7058153 7058065 ID=cds.MS.gene31224.t1;Parent=MS.gene31224.t1
chr2.4 exon 7058258 7058522 7058258 ID=MS.gene31224.t1.exon8;Parent=MS.gene31224.t1
chr2.4 CDS 7058258 7058522 7058258 ID=cds.MS.gene31224.t1;Parent=MS.gene31224.t1
chr2.4 exon 7058606 7058739 7058606 ID=MS.gene31224.t1.exon9;Parent=MS.gene31224.t1
chr2.4 CDS 7058606 7058739 7058606 ID=cds.MS.gene31224.t1;Parent=MS.gene31224.t1
chr2.4 exon 7058866 7058988 7058866 ID=MS.gene31224.t1.exon10;Parent=MS.gene31224.t1
chr2.4 CDS 7058866 7058988 7058866 ID=cds.MS.gene31224.t1;Parent=MS.gene31224.t1
Gene Sequence

>MS.gene31224

ATGGCATCATCTTTAAGGTTCCTCCAAATGTGTCTGTCTTTTACAATGCTGTTGCACTTCCTCTTCTTCATTTCACCTTCATTCTCCTTTCTTCAACTGCATCCCCTTCAACTCTCATAAGAGGACTTAACCTTTTTCCTAAAAGTTCCATAAACATACCAGAGAATGATCCTCATGTCCTCCATGGAAACATTGTGGAGAAGAAGTTCACTTTCCCTGGTTTTGATGACTCTGGTTATTCTGTTGAAGAACTTGGTCATCATGCAGGGTATTATAGTCTTCCTCATTCCAAAGCTGCTAGGTAAATGGTTTATCACTTTATGTCACCCCACATCATAATAACGTGTGCATGGACATATTTCCATTGAACAATTTGTTATGTCAATTGCAGGATGTTCTATTTTTTCTTTGAATCTCGAAACAGTAAGGATGACCCTGTCGTCATATGGTTAACTGGAGGTCCAGGATGCGGCAGTGAAATTGCCTTGTTTTATGAAAATGGACCTTTTCAGTTCTCAAAGGACAAGAACTTGTCCCTTGTTTGGAATGAATATGGATGGGACAAGGTAGAAGCTATTTATTCTTCCCTTTTCTTTTTTCCACTTTAATTCTCACTGATACATAATCATCTTGCAACATATACTTTTTTCTTTCTCTTTTGTAGGCATCAAATATTATATTTGTAGACCAACCCATTGGATCAGGTTTCAACTATACCACCGATGTTCACAGTGATATTCGCCGTGATGAAGACAGTATTGGCAACGATTTGTATGACTTCTTGCAGGTAACTTGATTTGTTCAGTACTTTATTAATTGATTAATCACTTGAATTAAGTGGTTAAATGACTTTTTTTTTTACGAGAAATTAAGTGGTTAAACTTGATTCATTGTTAGTTAGTACTTATATTTAATTTTAAAATTCAAAGACGACACTACATACTATCTATGGTTCTAAACTACAAATATTATGTTAAACTATGATGTTGTGCAAACATAAGATCAACTTAAATTTTTACAATGACAGGCATTTTTCAAGGAACACCCTCAGTTCACAAAGAATGATTTTTATATTACCGGAGAGTCATACGCTGGACATTACATTCCAGCTTTTGCATCCAGGGTTCGCCAAGGAAACAAAGCAAAAGAAGGAATTCATATAAATCTAAAGGTATTATTATTAGTATTACATTGTCATATAATATAACTGTATATTCATACAAAGGTAAACTTTATATCAAAGTAATGCAAACTAATCACATCGATTGTATTAACTCCTTTCATCTTTTATAATAAACAGGGCTTTGCTATTGGTAATGGATTAACCAATCCTGAAATTCAGTACATGGCATATACAGATTATGCATTAGGCAATGGACTGATTAATAAGGATGAATATGAAAGAATCAACAAGTTGATCCCACCGTGTCTAAAGGCGGCAGAAAAATGTGGTATAACATTGAGCTTTTTTCTCTTTCTCAGTCACAAATCACTTAGCACCAAGAGAGAATTTTACCAATTTATTGTGCAAGTCATGTGTTATTTTTCCTTATTGACACTCTTAATTTATCTGTTAGGCACTGAAGGTGGACAAGCATGTGGGACTGCATTAACTACTTGTATGAAAATATTCGATCAGATCACAAACATCACCGGCAACACTAACGTATGCTGTCAAAAAAGAAAAGTAGCACTAAAAGTATAAGGTAAAGATTGTATGAAAATAATGAAGTTTGAATACACTAATGCATTATGTTATTCGAACTGCAGTACTACGACATTAGAAAGAAATGTGAGGGAGCTTTCGGTGATTGTCAGGACTTCTCTGACATGGAGACATTCTTGAACATGAAGACAGTAAAGGAAGCTTTAGGTGTTGGGGACTTGAAATTTGATTCTTGCAGTTTCTTAGTACACGCTGCCTTGACGGAAGATTGGATGAAAAATCTTGAAGTGGGTATTCCTGCTCTTCTTGAGGATGGAATCAAGTTACTTGTGTACGCCGGAGAAAAAGATCTAATATGCAATTGGCTTGGTAAGCTCAATTAATTTTTCCAATCTTAAACAAGATACAAATTAGTTAGTTATAGAACTCTAAATGTGCTTCTCATTTGGTAGGGAATTCAAGGTGGGTTGATGCCATGAAGTGGTCAGGTCAGACAACATTTAAGGAGTCTCCTACAACTTCATTTTTGGTTGATAGTGAAGAAGCAGGAATTCTGAAAAATCATGGACCTTTGGCTTTCCTTAAGGTTTGTGTTTAAGGATGTATTCATGAGTATAAGAGTCTATTGATCTACATAGTACAGACGCTTCAAATTAAAAGTGTGTCTGGTGTTCGACATGTACTTAGCTATATATATTTTTTGGAACAATAGGTGAAAGAGGCTGGTCACATGGTTCCTATGGATCAACCAAAAGTTGCACTTCAAATGTTACAAGATTGGATGCAAGGGAAACTGCACTCAGCACAAAACCAAAATCACTTTCTTGTTGTATAA
Protein sequence

>MS.gene31224.t1

MASSLSSINIPENDPHVLHGNIVEKKFTFPGFDDSGYSVEELGHHAGYYSLPHSKAARMFYFFFESRNSKDDPVVIWLTGGPGCGSEIALFYENGPFQFSKDKNLSLVWNEYGWDKASNIIFVDQPIGSGFNYTTDVHSDIRRDEDSIGNDLYDFLQAFFKEHPQFTKNDFYITGESYAGHYIPAFASRVRQGNKAKEGIHINLKGFAIGNGLTNPEIQYMAYTDYALGNGLINKDEYERINKLIPPCLKAAEKCGTEGGQACGTALTTCMKIFDQITNITGNTNYYDIRKKCEGAFGDCQDFSDMETFLNMKTVKEALGVGDLKFDSCSFLVHAALTEDWMKNLEVGIPALLEDGIKLLVYAGEKDLICNWLGNSRWVDAMKWSGQTTFKESPTTSFLVDSEEAGILKNHGPLAFLKVKEAGHMVPMDQPKVALQMLQDWMQGKLHSAQNQNHFLVV