Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32257.t1 | XP_013443169.1 | 97 | 904 | 26 | 1 | 1 | 904 | 1 | 903 | 0.00E+00 | 1746.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32257.t1 | Q9SYM4 | 75.0 | 925 | 205 | 7 | 1 | 904 | 1 | 920 | 0.0e+00 | 1353.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32257.t1 | A0A072TI12 | 97.0 | 904 | 26 | 1 | 1 | 904 | 1 | 903 | 0.0e+00 | 1746.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050704 | MS.gene32257 | 0.830677 | 2.57E-55 | -1.69E-46 |
| MS.gene051509 | MS.gene32257 | 0.801154 | 1.03E-48 | -1.69E-46 |
| MS.gene052413 | MS.gene32257 | 0.804406 | 2.19E-49 | -1.69E-46 |
| MS.gene052966 | MS.gene32257 | 0.811008 | 8.68E-51 | -1.69E-46 |
| MS.gene053318 | MS.gene32257 | 0.83478 | 2.46E-56 | -1.69E-46 |
| MS.gene05492 | MS.gene32257 | 0.854609 | 1.10E-61 | -1.69E-46 |
| MS.gene05601 | MS.gene32257 | 0.81124 | 7.73E-51 | -1.69E-46 |
| MS.gene056166 | MS.gene32257 | 0.81388 | 2.05E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene32257 | 0.807047 | 6.12E-50 | -1.69E-46 |
| MS.gene059367 | MS.gene32257 | 0.800143 | 1.66E-48 | -1.69E-46 |
| MS.gene059517 | MS.gene32257 | 0.811032 | 8.57E-51 | -1.69E-46 |
| MS.gene060837 | MS.gene32257 | 0.802807 | 4.71E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32257.t1 | MTR_0034s0170 | 98.673 | 904 | 11 | 1 | 1 | 904 | 1 | 903 | 0.0 | 1863 |
| MS.gene32257.t1 | MTR_2g073260 | 74.289 | 949 | 184 | 11 | 1 | 905 | 9 | 941 | 0.0 | 1401 |
| MS.gene32257.t1 | MTR_8g087930 | 70.495 | 505 | 133 | 7 | 406 | 904 | 9 | 503 | 0.0 | 711 |
| MS.gene32257.t1 | MTR_8g087910 | 85.714 | 322 | 39 | 1 | 84 | 405 | 75 | 389 | 0.0 | 589 |
| MS.gene32257.t1 | MTR_1g109620 | 35.369 | 786 | 431 | 22 | 54 | 797 | 42 | 792 | 1.88e-142 | 446 |
| MS.gene32257.t1 | MTR_1g032730 | 36.780 | 764 | 410 | 25 | 83 | 797 | 60 | 799 | 7.41e-142 | 445 |
| MS.gene32257.t1 | MTR_8g105740 | 35.622 | 772 | 429 | 25 | 84 | 808 | 59 | 809 | 4.79e-138 | 435 |
| MS.gene32257.t1 | MTR_4g080160 | 34.085 | 754 | 432 | 21 | 86 | 797 | 67 | 797 | 6.39e-136 | 429 |
| MS.gene32257.t1 | MTR_4g129270 | 35.364 | 755 | 429 | 21 | 84 | 797 | 55 | 791 | 8.09e-136 | 429 |
| MS.gene32257.t1 | MTR_8g063790 | 34.539 | 802 | 431 | 23 | 84 | 830 | 52 | 814 | 5.11e-134 | 424 |
| MS.gene32257.t1 | MTR_4g129270 | 35.724 | 711 | 402 | 18 | 84 | 753 | 55 | 751 | 6.80e-130 | 410 |
| MS.gene32257.t1 | MTR_3g078210 | 32.673 | 707 | 417 | 19 | 129 | 797 | 26 | 711 | 3.14e-110 | 359 |
| MS.gene32257.t1 | MTR_3g467030 | 30.172 | 580 | 344 | 16 | 87 | 637 | 1 | 548 | 1.40e-63 | 226 |
| MS.gene32257.t1 | MTR_3g467040 | 28.899 | 218 | 103 | 3 | 329 | 546 | 2 | 167 | 1.65e-16 | 80.5 |
| MS.gene32257.t1 | MTR_7g059155 | 40.909 | 110 | 53 | 6 | 875 | 977 | 10 | 114 | 3.42e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32257.t1 | AT1G78580 | 76.432 | 925 | 192 | 8 | 1 | 904 | 1 | 920 | 0.0 | 1418 |
| MS.gene32257.t1 | AT1G78580 | 76.432 | 925 | 192 | 8 | 1 | 904 | 1 | 920 | 0.0 | 1418 |
| MS.gene32257.t1 | AT1G78580 | 76.432 | 925 | 192 | 8 | 1 | 904 | 1 | 920 | 0.0 | 1418 |
| MS.gene32257.t1 | AT1G78580 | 76.432 | 925 | 192 | 8 | 1 | 904 | 1 | 920 | 0.0 | 1418 |
| MS.gene32257.t1 | AT1G16980 | 66.713 | 724 | 234 | 4 | 84 | 806 | 11 | 728 | 0.0 | 1045 |
| MS.gene32257.t1 | AT4G27550 | 66.205 | 722 | 240 | 3 | 84 | 804 | 3 | 721 | 0.0 | 1007 |
| MS.gene32257.t1 | AT4G27550 | 65.527 | 731 | 239 | 4 | 84 | 804 | 3 | 730 | 0.0 | 1000 |
| MS.gene32257.t1 | AT1G16980 | 65.781 | 602 | 201 | 3 | 84 | 684 | 11 | 608 | 0.0 | 860 |
| MS.gene32257.t1 | AT4G17770 | 35.279 | 788 | 434 | 21 | 51 | 797 | 40 | 792 | 2.07e-144 | 452 |
| MS.gene32257.t1 | AT4G17770 | 35.279 | 788 | 434 | 21 | 51 | 797 | 40 | 792 | 2.07e-144 | 452 |
| MS.gene32257.t1 | AT1G23870 | 34.890 | 771 | 430 | 21 | 84 | 805 | 58 | 805 | 6.65e-138 | 435 |
| MS.gene32257.t1 | AT1G70290 | 34.656 | 756 | 434 | 18 | 81 | 797 | 53 | 787 | 5.17e-137 | 432 |
| MS.gene32257.t1 | AT1G06410 | 33.912 | 749 | 436 | 18 | 86 | 797 | 60 | 786 | 7.68e-137 | 432 |
| MS.gene32257.t1 | AT1G06410 | 33.912 | 749 | 436 | 18 | 86 | 797 | 60 | 786 | 7.68e-137 | 432 |
| MS.gene32257.t1 | AT1G06410 | 33.912 | 749 | 436 | 18 | 86 | 797 | 60 | 786 | 7.68e-137 | 432 |
| MS.gene32257.t1 | AT1G68020 | 34.094 | 789 | 444 | 18 | 66 | 797 | 34 | 803 | 1.16e-136 | 431 |
| MS.gene32257.t1 | AT1G68020 | 34.094 | 789 | 444 | 18 | 66 | 797 | 34 | 803 | 1.16e-136 | 431 |
| MS.gene32257.t1 | AT1G60140 | 34.557 | 790 | 447 | 21 | 84 | 830 | 58 | 820 | 4.72e-133 | 422 |
| MS.gene32257.t1 | AT1G60140 | 34.557 | 790 | 447 | 21 | 84 | 830 | 58 | 820 | 4.72e-133 | 422 |
| MS.gene32257.t1 | AT1G60140 | 34.557 | 790 | 447 | 21 | 84 | 830 | 58 | 820 | 4.72e-133 | 422 |
| MS.gene32257.t1 | AT1G60140 | 34.557 | 790 | 447 | 21 | 84 | 830 | 58 | 820 | 4.72e-133 | 422 |
| MS.gene32257.t1 | AT1G60140 | 34.557 | 790 | 447 | 21 | 84 | 830 | 58 | 820 | 4.72e-133 | 422 |
| MS.gene32257.t1 | AT2G18700 | 34.061 | 825 | 465 | 28 | 84 | 853 | 49 | 849 | 8.87e-130 | 413 |
| MS.gene32257.t1 | AT1G60140 | 35.196 | 716 | 397 | 18 | 84 | 753 | 58 | 752 | 3.72e-126 | 401 |
| MS.gene32257.t1 | AT1G70290 | 32.314 | 752 | 419 | 18 | 81 | 797 | 53 | 749 | 8.08e-116 | 375 |
| MS.gene32257.t1 | AT1G68020 | 33.883 | 667 | 373 | 13 | 66 | 675 | 34 | 689 | 1.86e-115 | 370 |
Find 231 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTGCAATCAGACGTTATT+TGG | 0.145033 | 8.3:+48573242 | MS.gene32257:CDS |
| GTTCCTTCCAAAATGCTTAA+AGG | 0.148592 | 8.3:+48569344 | MS.gene32257:CDS |
| TTCAAGGGTTGTCTCAATTT+TGG | 0.152601 | 8.3:-48573801 | None:intergenic |
| AACTAATAGATTGCTCATTT+TGG | 0.170950 | 8.3:+48573269 | MS.gene32257:CDS |
| TGAATCTTTCTTGCAATTCT+TGG | 0.180059 | 8.3:-48569918 | None:intergenic |
| TTATGTTCCTTTAAGCATTT+TGG | 0.189058 | 8.3:-48569351 | None:intergenic |
| TTTCAAGCAGATGTTGAATT+TGG | 0.192249 | 8.3:+48574357 | MS.gene32257:intron |
| GTCGAGTAGAAAGGCTATTA+AGG | 0.199061 | 8.3:+48566236 | MS.gene32257:CDS |
| GATCGCCTTGATATGATTAA+AGG | 0.212944 | 8.3:+48570086 | MS.gene32257:CDS |
| GGACTGGAGGGTACACCTTA+TGG | 0.221278 | 8.3:+48569622 | MS.gene32257:CDS |
| AGTTGAGGAAAAGTGGAAAT+AGG | 0.228400 | 8.3:+48566266 | MS.gene32257:CDS |
| TAGAGCCTCCCTAGCTTTCT+TGG | 0.242221 | 8.3:-48576101 | None:intergenic |
| GAGGTGTATTCCCGTATTTC+TGG | 0.257136 | 8.3:+48569101 | MS.gene32257:intron |
| GACTGGAGGGTACACCTTAT+GGG | 0.275216 | 8.3:+48569623 | MS.gene32257:CDS |
| AGAAGTAATTATCTTTATTA+AGG | 0.275466 | 8.3:-48575693 | None:intergenic |
| AGCACAACTACTGTGGTATT+TGG | 0.277592 | 8.3:-48573840 | None:intergenic |
| AGAGGGAAACTTCACTTGTT+TGG | 0.297016 | 8.3:+48574239 | MS.gene32257:CDS |
| GAGCCGGATCTTCCATCTAT+TGG | 0.307665 | 8.3:+48575442 | MS.gene32257:CDS |
| GGTCGCATCAATGGCAGATT+TGG | 0.307807 | 8.3:+48570510 | MS.gene32257:CDS |
| GTTGAGGAAAAGTGGAAATA+GGG | 0.310351 | 8.3:+48566267 | MS.gene32257:CDS |
| AGTGCTTGTACTCGCATTCT+TGG | 0.320512 | 8.3:+48569601 | MS.gene32257:CDS |
| GCTATTGCAACAATATATTA+TGG | 0.323774 | 8.3:+48569154 | MS.gene32257:CDS |
| TCTGTTGTTGCCTTACTTCC+CGG | 0.326971 | 8.3:-48575475 | None:intergenic |
| AAAATATAAACAAAGGCTTT+TGG | 0.331201 | 8.3:+48566423 | MS.gene32257:CDS |
| TAACGTACCAGGTGATTAAT+AGG | 0.342851 | 8.3:-48570549 | None:intergenic |
| GTATGCTTCATGTAGTTTGC+CGG | 0.343432 | 8.3:+48571996 | MS.gene32257:intron |
| TCTATTGGTGTGAGTCTTCC+GGG | 0.344284 | 8.3:+48575457 | MS.gene32257:CDS |
| TGTGCTTTGTATGCAGTTAC+TGG | 0.347837 | 8.3:+48571064 | MS.gene32257:CDS |
| AATGCCCGAGCATCTGAATA+TGG | 0.348611 | 8.3:+48574055 | MS.gene32257:CDS |
| TTCCTTCTTCTGAAATTCAT+AGG | 0.352662 | 8.3:+48569412 | MS.gene32257:CDS |
| ATCTATTGGTGTGAGTCTTC+CGG | 0.355274 | 8.3:+48575456 | MS.gene32257:CDS |
| CATTTGATTGGCTTTCTCAT+AGG | 0.359196 | 8.3:-48569253 | None:intergenic |
| GTTCTTGCAGCTGATTTAGT+TGG | 0.360827 | 8.3:+48569468 | MS.gene32257:CDS |
| TGAGGCAAGGTGGATAGGTT+GGG | 0.362964 | 8.3:+48568760 | MS.gene32257:CDS |
| AGCTACATCTGTAATGTTCC+AGG | 0.364914 | 8.3:-48572060 | None:intergenic |
| GGAGCCGATCTGTTGAAGTT+AGG | 0.366819 | 8.3:+48574457 | MS.gene32257:CDS |
| AACATCAGCAAACATTTGAT+TGG | 0.368245 | 8.3:-48569265 | None:intergenic |
| GAATTGCTTGACTTGTATCT+TGG | 0.370919 | 8.3:-48576045 | None:intergenic |
| CAGGAACATCTGTTCTTGTT+GGG | 0.382634 | 8.3:-48570196 | None:intergenic |
| ATCAAGTAATAATAATCAAA+TGG | 0.383362 | 8.3:+48566297 | MS.gene32257:CDS |
| TTGAGGCAAGGTGGATAGGT+TGG | 0.386014 | 8.3:+48568759 | MS.gene32257:CDS |
| GATTGCCTGTGTCGGCAAAT+AGG | 0.387008 | 8.3:+48566458 | MS.gene32257:CDS |
| ATCACTGCATAGTGCTTTCA+AGG | 0.389206 | 8.3:-48573817 | None:intergenic |
| ATAGGACAGGTGAAGATTCT+TGG | 0.389444 | 8.3:+48566476 | MS.gene32257:CDS |
| CACTAAAGCATTGGCTGAAA+AGG | 0.389935 | 8.3:+48568823 | MS.gene32257:CDS |
| GCCGGTGCAGCCCAGTCTCT+TGG | 0.390692 | 8.3:+48572014 | MS.gene32257:CDS |
| CAACGGGACTTTAACTGAAC+CGG | 0.391025 | 8.3:+48573731 | MS.gene32257:CDS |
| GTCATCCACTCACCCTTTGA+AGG | 0.391983 | 8.3:-48574029 | None:intergenic |
| TTGCTCCAGCACCAAGAGAC+TGG | 0.400447 | 8.3:-48572025 | None:intergenic |
| AATTTCTTCATCCAGAAATA+CGG | 0.402618 | 8.3:-48569112 | None:intergenic |
| GCAGCCCAGTCTCTTGGTGC+TGG | 0.404371 | 8.3:+48572020 | MS.gene32257:CDS |
| TCAGGAACATCTGTTCTTGT+TGG | 0.406315 | 8.3:-48570197 | None:intergenic |
| AAAGCCGGTTTCTTCTCAAC+AGG | 0.408185 | 8.3:-48575511 | None:intergenic |
| GATTAAAGAAATGGAACTTA+AGG | 0.412913 | 8.3:+48573773 | MS.gene32257:CDS |
| AATTGCAAGGGCATTGAATA+TGG | 0.414467 | 8.3:+48572088 | MS.gene32257:CDS |
| ATTACTTCTCATGTTCTGTT+GGG | 0.415836 | 8.3:+48575707 | MS.gene32257:CDS |
| AGGTGATCAGATTAAAGAAA+TGG | 0.416656 | 8.3:+48573764 | MS.gene32257:CDS |
| GAGGGACTTGGAGGACAAAA+TGG | 0.417256 | 8.3:+48576166 | MS.gene32257:CDS |
| GAGAGAGAGTTGAGGAAAAG+TGG | 0.418006 | 8.3:+48566259 | MS.gene32257:CDS |
| CATGAAGAACATGATGTTGT+TGG | 0.419800 | 8.3:+48566400 | MS.gene32257:CDS |
| AAAGTCATCATCTCAAACTA+AGG | 0.425552 | 8.3:+48575567 | MS.gene32257:CDS |
| TTATGAATTTGTGGCTTGCC+AGG | 0.425900 | 8.3:+48571388 | MS.gene32257:CDS |
| CCCAGATGAGGTTGGACAAA+AGG | 0.427644 | 8.3:+48568796 | MS.gene32257:CDS |
| TTCTTGCAGCTGATTTAGTT+GGG | 0.428507 | 8.3:+48569469 | MS.gene32257:CDS |
| TCTTGTCAGTTATGAATTTG+TGG | 0.430611 | 8.3:+48571379 | MS.gene32257:CDS |
| CATCAAACCATTGGGAGGTT+GGG | 0.434339 | 8.3:+48576196 | MS.gene32257:CDS |
| TCAACTGATGAATTAGAAAT+CGG | 0.435854 | 8.3:-48574423 | None:intergenic |
| AATTACTTCTCATGTTCTGT+TGG | 0.435910 | 8.3:+48575706 | MS.gene32257:CDS |
| GAAATGTCTTGCATAATCAT+AGG | 0.435989 | 8.3:-48569575 | None:intergenic |
| TGAATTTGACAGGTAATGTT+GGG | 0.436984 | 8.3:+48570059 | MS.gene32257:intron |
| TAGCGCTTGTCACATCGTTA+AGG | 0.438058 | 8.3:+48571345 | MS.gene32257:CDS |
| AGCAGGATGCCGTTGTGAAC+TGG | 0.443405 | 8.3:-48575641 | None:intergenic |
| GGAAGACTCACACCAATAGA+TGG | 0.447348 | 8.3:-48575454 | None:intergenic |
| AGCGCTTGTCACATCGTTAA+GGG | 0.448573 | 8.3:+48571346 | MS.gene32257:CDS |
| TGAATTTCAGAAGAAGGAAA+AGG | 0.449169 | 8.3:-48569408 | None:intergenic |
| AGCAGCTATTGACCGCATCC+TGG | 0.450206 | 8.3:+48574733 | MS.gene32257:CDS |
| ATCCCACACTGCACAAGAAT+GGG | 0.450952 | 8.3:+48572160 | MS.gene32257:CDS |
| CCTTGATACTCAACCCCATA+AGG | 0.452656 | 8.3:-48569637 | None:intergenic |
| ATGAAGAGGGTGATGTTGTT+TGG | 0.453479 | 8.3:+48569301 | MS.gene32257:CDS |
| GAAATTGTTGGTCGCATCAA+TGG | 0.454672 | 8.3:+48570501 | MS.gene32257:CDS |
| AGCCCATTCTTGTGCAGTGT+GGG | 0.455106 | 8.3:-48572162 | None:intergenic |
| ACTGAACCGGTTGAGAAAAC+AGG | 0.456154 | 8.3:+48573744 | MS.gene32257:CDS |
| GGCTAGGAAGATCAATGATA+AGG | 0.456918 | 8.3:+48576071 | MS.gene32257:CDS |
| GCATCAAACCATTGGGAGGT+TGG | 0.465790 | 8.3:+48576195 | MS.gene32257:CDS |
| TCGAGTAGAAAGGCTATTAA+GGG | 0.466944 | 8.3:+48566237 | MS.gene32257:CDS |
| ATGCAGAGAACATAATCAAT+TGG | 0.467240 | 8.3:-48574788 | None:intergenic |
| ATGTGGTTGGTAGCAGAGAA+TGG | 0.468842 | 8.3:+48573993 | MS.gene32257:CDS |
| GAATTTCAGAAGAAGGAAAA+GGG | 0.471870 | 8.3:-48569407 | None:intergenic |
| ACCAAGAGACTGGGCTGCAC+CGG | 0.472776 | 8.3:-48572015 | None:intergenic |
| GAGCCGATCTGTTGAAGTTA+GGG | 0.473081 | 8.3:+48574458 | MS.gene32257:CDS |
| GTCCTATGAATTTCAGAAGA+AGG | 0.474257 | 8.3:-48569414 | None:intergenic |
| GTTTGGAATTACAAATATGC+AGG | 0.474578 | 8.3:+48574256 | MS.gene32257:CDS |
| TCATCAGTTGAAGTTGTTCA+AGG | 0.479515 | 8.3:+48574435 | MS.gene32257:CDS |
| GGATTCCTTTAATCATATCA+AGG | 0.479966 | 8.3:-48570091 | None:intergenic |
| TTGAATTTGACAGGTAATGT+TGG | 0.482088 | 8.3:+48570058 | MS.gene32257:intron |
| TCAAGTAATAATAATCAAAT+GGG | 0.482886 | 8.3:+48566298 | MS.gene32257:CDS |
| GGTCAATTGCATCAAACCAT+TGG | 0.483563 | 8.3:+48576187 | MS.gene32257:CDS |
| CCTTATGGGGTTGAGTATCA+AGG | 0.484862 | 8.3:+48569637 | MS.gene32257:CDS |
| GAAGGAGCATAAAATATGGC+AGG | 0.486089 | 8.3:+48566166 | None:intergenic |
| GGGTGATGTATATTTAGAAC+AGG | 0.486332 | 8.3:+48566318 | MS.gene32257:CDS |
| GTCAATTGCATCAAACCATT+GGG | 0.487020 | 8.3:+48576188 | MS.gene32257:CDS |
| AATCCCACACTGCACAAGAA+TGG | 0.487441 | 8.3:+48572159 | MS.gene32257:CDS |
| TGCTCCAGCACCAAGAGACT+GGG | 0.489531 | 8.3:-48572024 | None:intergenic |
| TTGAATGTCAGTTTCCGATC+GGG | 0.491301 | 8.3:+48569839 | MS.gene32257:intron |
| CTTGAATGTCAGTTTCCGAT+CGG | 0.492040 | 8.3:+48569838 | MS.gene32257:intron |
| GATACAAGTCAAGCAATTCA+AGG | 0.497211 | 8.3:+48576049 | MS.gene32257:CDS |
| CATCAGTTGAAGTTGTTCAA+GGG | 0.497269 | 8.3:+48574436 | MS.gene32257:CDS |
| TGTGAGTCTTCCGGGAAGTA+AGG | 0.497638 | 8.3:+48575465 | MS.gene32257:CDS |
| AGCCAGGTTCATGAAATTGT+TGG | 0.498362 | 8.3:+48570489 | MS.gene32257:CDS |
| CCATTCCATATTCAGATGCT+CGG | 0.499121 | 8.3:-48574060 | None:intergenic |
| GTTTCCTGTTGAGAAGAAAC+CGG | 0.499522 | 8.3:+48575507 | MS.gene32257:CDS |
| CATAACAGTAAAATGAAAGT+TGG | 0.501724 | 8.3:+48569369 | MS.gene32257:CDS |
| GAGCAATTCTTGTCAACCCC+TGG | 0.502288 | 8.3:+48572042 | MS.gene32257:CDS |
| ATTGATTATGTTCTCTGCAT+AGG | 0.504053 | 8.3:+48574791 | MS.gene32257:CDS |
| AGAAAGATTCAAAGGAAGAA+AGG | 0.505938 | 8.3:+48569930 | MS.gene32257:CDS |
| CGTCTGATTGCAATCTCAGT+TGG | 0.506309 | 8.3:-48573234 | None:intergenic |
| CTTGTGGAAGTCCAAGGTAA+TGG | 0.507430 | 8.3:-48569185 | None:intergenic |
| AATTATCTTTATTAAGGTCA+AGG | 0.507894 | 8.3:-48575687 | None:intergenic |
| GCTAGGAAGATCAATGATAA+GGG | 0.508831 | 8.3:+48576072 | MS.gene32257:CDS |
| TAGGGAGGCTCTAAATCAAA+TGG | 0.510539 | 8.3:+48576111 | MS.gene32257:CDS |
| ATTTCTTCATCCAGAAATAC+GGG | 0.510861 | 8.3:-48569111 | None:intergenic |
| GCAAGGTGGATAGGTTGGGC+TGG | 0.512257 | 8.3:+48568764 | MS.gene32257:CDS |
| ACAGTAAAATGAAAGTTGGC+TGG | 0.514997 | 8.3:+48569373 | MS.gene32257:CDS |
| TGGATATGTTTCTGCACTTG+AGG | 0.516120 | 8.3:-48569898 | None:intergenic |
| AGTCAAGCAATTCAAGGGCT+AGG | 0.519818 | 8.3:+48576055 | MS.gene32257:CDS |
| TGGTCTTTAGAGATCAGTGC+AGG | 0.520199 | 8.3:+48566496 | MS.gene32257:CDS |
| ATAATCAATTGGTGATGTCA+TGG | 0.523102 | 8.3:-48574777 | None:intergenic |
| TTAACGTACAGGGATTCAAC+GGG | 0.524323 | 8.3:+48573715 | MS.gene32257:intron |
| GTAGTTGTGCTCAGTGGAAG+TGG | 0.524618 | 8.3:+48573852 | MS.gene32257:CDS |
| CCGAGCATCTGAATATGGAA+TGG | 0.525472 | 8.3:+48574060 | MS.gene32257:CDS |
| TCGGACCTGTCCACAGATGT+TGG | 0.525504 | 8.3:-48574404 | None:intergenic |
| TTGAGGAAAAGTGGAAATAG+GGG | 0.527558 | 8.3:+48566268 | MS.gene32257:CDS |
| ACTGGAGGGTACACCTTATG+GGG | 0.528431 | 8.3:+48569624 | MS.gene32257:CDS |
| TACTCGCATTCTTGGACTGG+AGG | 0.530186 | 8.3:+48569609 | MS.gene32257:CDS |
| CATTCCATATTCAGATGCTC+GGG | 0.531899 | 8.3:-48574059 | None:intergenic |
| CAGCCCATTCTTGTGCAGTG+TGG | 0.534186 | 8.3:-48572163 | None:intergenic |
| TTCTGTTCCTATTAATCACC+TGG | 0.534644 | 8.3:+48570542 | MS.gene32257:CDS |
| ATTAACGTACAGGGATTCAA+CGG | 0.535220 | 8.3:+48573714 | MS.gene32257:intron |
| TGAAGCGAAGACAACAATAG+AGG | 0.535366 | 8.3:+48576147 | MS.gene32257:CDS |
| CGAGCATCTGAATATGGAAT+GGG | 0.537588 | 8.3:+48574061 | MS.gene32257:CDS |
| TATGTTTCTGCACTTGAGGA+AGG | 0.537816 | 8.3:-48569894 | None:intergenic |
| AGGCAAGAATTACATCATCT+AGG | 0.542820 | 8.3:-48573884 | None:intergenic |
| TTTCCGATCGGGATAGACTC+TGG | 0.544659 | 8.3:+48569850 | MS.gene32257:CDS |
| TACCACCAGGTCGAGTAGAA+AGG | 0.545239 | 8.3:+48566227 | MS.gene32257:CDS |
| TTGTACTCGCATTCTTGGAC+TGG | 0.546130 | 8.3:+48569606 | MS.gene32257:CDS |
| GGGCACATACCAAGAAAGCT+AGG | 0.546540 | 8.3:+48576092 | MS.gene32257:CDS |
| AACATCTGTTCTTGTTGGGA+CGG | 0.547223 | 8.3:-48570192 | None:intergenic |
| ACTAAATGACACTGTTATTG+AGG | 0.547617 | 8.3:+48573176 | MS.gene32257:CDS |
| TCTGTTGAAGTTAGGGCTGT+TGG | 0.550368 | 8.3:+48574465 | MS.gene32257:CDS |
| GACCAACAATTTCATGAACC+TGG | 0.550436 | 8.3:-48570491 | None:intergenic |
| ACACCAATAGATGGAAGATC+CGG | 0.551065 | 8.3:-48575445 | None:intergenic |
| TTGTTTAACAAAAGGTGTGA+AGG | 0.551103 | 8.3:+48568733 | MS.gene32257:intron |
| GCAGCTATTGACCGCATCCT+GGG | 0.551609 | 8.3:+48574734 | MS.gene32257:CDS |
| ACTAAATAAAATGAGGAGCA+TGG | 0.553038 | 8.3:-48576225 | None:intergenic |
| CTGCTCCAGAAAATTCATCA+TGG | 0.555004 | 8.3:+48575659 | MS.gene32257:CDS |
| ATGTAGCTAGTGCAATTGCA+AGG | 0.556459 | 8.3:+48572075 | MS.gene32257:CDS |
| GGCCTAGTCAGTGCTCTCCT+AGG | 0.557844 | 8.3:+48566520 | MS.gene32257:CDS |
| GCTACATCTGTAATGTTCCA+GGG | 0.558914 | 8.3:-48572059 | None:intergenic |
| CACACTGCACAAGAATGGGC+TGG | 0.561652 | 8.3:+48572164 | MS.gene32257:CDS |
| AAAGGCACTCACTAAAGCAT+TGG | 0.561692 | 8.3:+48568814 | MS.gene32257:CDS |
| GTAGCAAGACGGTCTTCTTG+TGG | 0.562961 | 8.3:-48569201 | None:intergenic |
| TGGTATGTGATGATGATGAT+TGG | 0.563353 | 8.3:-48566209 | None:intergenic |
| ACAACAATAGAGGGACTTGG+AGG | 0.564749 | 8.3:+48576157 | MS.gene32257:CDS |
| TACAACCTTCAAAGGGTGAG+TGG | 0.567297 | 8.3:+48574024 | MS.gene32257:CDS |
| AGTCCAAGGTAATGGAAAAG+TGG | 0.567629 | 8.3:-48569177 | None:intergenic |
| TATTAAGGGAGAGAGAGTTG+AGG | 0.569696 | 8.3:+48566251 | MS.gene32257:CDS |
| GTTGGGGTGATCTTCAAAGC+CGG | 0.571773 | 8.3:-48575526 | None:intergenic |
| ATGCTCCAACATCTGTGGAC+AGG | 0.574663 | 8.3:+48574399 | MS.gene32257:CDS |
| CATCACCCTCTTCATAGTGT+TGG | 0.578517 | 8.3:-48569293 | None:intergenic |
| AAGAACTGAACGCAGAAGCT+CGG | 0.578763 | 8.3:-48569451 | None:intergenic |
| TGTCTTCGCTTCAATGATTG+TGG | 0.579472 | 8.3:-48576137 | None:intergenic |
| GAACAATCTCACCCAGGATG+CGG | 0.579534 | 8.3:-48574745 | None:intergenic |
| CCTGTGTCGGCAAATAGGAC+AGG | 0.581319 | 8.3:+48566463 | MS.gene32257:CDS |
| ACACAGCACTAAATAAAATG+AGG | 0.583977 | 8.3:-48576232 | None:intergenic |
| CCTGTCCTATTTGCCGACAC+AGG | 0.585198 | 8.3:-48566463 | None:intergenic |
| TGTGGTTGGTAGCAGAGAAT+GGG | 0.587128 | 8.3:+48573994 | MS.gene32257:CDS |
| AAGACAACAATAGAGGGACT+TGG | 0.591805 | 8.3:+48576154 | MS.gene32257:CDS |
| AGATCAAAAGCTTACAAGCC+AGG | 0.592894 | 8.3:+48570473 | MS.gene32257:intron |
| GGAACATAAGATGGTAGTCA+TGG | 0.592950 | 8.3:-48569326 | None:intergenic |
| CTTATGGGGTTGAGTATCAA+GGG | 0.593811 | 8.3:+48569638 | MS.gene32257:CDS |
| GAATTGCAAGAAAGATTCAA+AGG | 0.598077 | 8.3:+48569922 | MS.gene32257:CDS |
| GGTCTTCTTGTGGAAGTCCA+AGG | 0.598351 | 8.3:-48569191 | None:intergenic |
| ATTGTTCATCAATATTACAA+TGG | 0.603082 | 8.3:+48569132 | MS.gene32257:CDS |
| ATACAAGTCAAGCAATTCAA+GGG | 0.603763 | 8.3:+48576050 | MS.gene32257:CDS |
| GGCACATACCAAGAAAGCTA+GGG | 0.607808 | 8.3:+48576093 | MS.gene32257:CDS |
| ATCTCAGTTGGAAGCCGAGG+CGG | 0.608203 | 8.3:-48573222 | None:intergenic |
| GTCAATGTCCCAGATGAGGT+TGG | 0.608709 | 8.3:+48568788 | MS.gene32257:CDS |
| TTGTTGGAAAATATAAACAA+AGG | 0.609981 | 8.3:+48566416 | MS.gene32257:CDS |
| GCAATCTCAGTTGGAAGCCG+AGG | 0.610794 | 8.3:-48573225 | None:intergenic |
| AATAGCCTTTCTACTCGACC+TGG | 0.611509 | 8.3:-48566232 | None:intergenic |
| TAGGGCTGTTGGTGTTACAA+AGG | 0.614666 | 8.3:+48574476 | MS.gene32257:CDS |
| TGGTGTCAATGTCCCAGATG+AGG | 0.614863 | 8.3:+48568784 | MS.gene32257:CDS |
| CGCAGAAGCTCGGAACGAGA+AGG | 0.618843 | 8.3:-48569441 | None:intergenic |
| AATTTCAGAAGAAGGAAAAG+GGG | 0.619528 | 8.3:-48569406 | None:intergenic |
| CAGCCCTAACTTCAACAGAT+CGG | 0.621852 | 8.3:-48574461 | None:intergenic |
| AAGGAGCATAAAATATGGCA+GGG | 0.622183 | 8.3:+48566167 | None:intergenic |
| GAGACATGCTCCAACATCTG+TGG | 0.622658 | 8.3:+48574394 | MS.gene32257:CDS |
| GAAGCGAAGACAACAATAGA+GGG | 0.622884 | 8.3:+48576148 | MS.gene32257:CDS |
| GGCAAATAGATTGCCTGTGT+CGG | 0.623416 | 8.3:+48566450 | MS.gene32257:CDS |
| ACTCGCATTCTTGGACTGGA+GGG | 0.626764 | 8.3:+48569610 | MS.gene32257:CDS |
| ATCAAATTTCCAGTTCACAA+CGG | 0.627590 | 8.3:+48575632 | MS.gene32257:CDS |
| TACCTAGGAGAGCACTGACT+AGG | 0.628819 | 8.3:-48566522 | None:intergenic |
| AATTGCATCAAACCATTGGG+AGG | 0.629282 | 8.3:+48576191 | MS.gene32257:CDS |
| CTACATCTGTAATGTTCCAG+GGG | 0.629786 | 8.3:-48572058 | None:intergenic |
| CATCATCATCACATACCACC+AGG | 0.630585 | 8.3:+48566214 | MS.gene32257:CDS |
| TCACTGCATAGTGCTTTCAA+GGG | 0.630952 | 8.3:-48573816 | None:intergenic |
| TCTTTAGAGATCAGTGCAGG+TGG | 0.631776 | 8.3:+48566499 | MS.gene32257:CDS |
| CGTCCAGAGTCTATCCCGAT+CGG | 0.633295 | 8.3:-48569853 | None:intergenic |
| TGCTATGAACAATCTCACCC+AGG | 0.640991 | 8.3:-48574751 | None:intergenic |
| TGTAGCTAGTGCAATTGCAA+GGG | 0.641479 | 8.3:+48572076 | MS.gene32257:CDS |
| GCATGCGCCCAACCTCCCAA+TGG | 0.641803 | 8.3:-48576203 | None:intergenic |
| GGAATGGGTCGACAGCGTGA+AGG | 0.642967 | 8.3:+48574076 | MS.gene32257:CDS |
| ACCACAGTAGTTGTGCTCAG+TGG | 0.650048 | 8.3:+48573846 | MS.gene32257:CDS |
| CTTGTCACATCGTTAAGGGA+CGG | 0.651489 | 8.3:+48571350 | MS.gene32257:CDS |
| CAAAGAATACGACATGTGGT+TGG | 0.652564 | 8.3:+48573980 | MS.gene32257:CDS |
| AGCCTTTCTACTCGACCTGG+TGG | 0.656847 | 8.3:-48566229 | None:intergenic |
| AACTACGCGTGGTAGCAAGA+CGG | 0.659719 | 8.3:-48569212 | None:intergenic |
| ACATACCAAGAAAGCTAGGG+AGG | 0.663957 | 8.3:+48576096 | MS.gene32257:CDS |
| GGACAAGACAAGTTCCGCCT+CGG | 0.664453 | 8.3:+48573208 | MS.gene32257:CDS |
| ACTTCAAAGAATACGACATG+TGG | 0.669733 | 8.3:+48573976 | MS.gene32257:CDS |
| CATCATCAATAGTAACTCAG+AGG | 0.670345 | 8.3:+48566374 | MS.gene32257:CDS |
| TCCACTGAGCACAACTACTG+TGG | 0.674131 | 8.3:-48573847 | None:intergenic |
| GTGAATGTGATAGTCTGATG+AGG | 0.679756 | 8.3:+48576012 | MS.gene32257:intron |
| TGAATGTGATAGTCTGATGA+GGG | 0.681443 | 8.3:+48576013 | MS.gene32257:intron |
| TCGAAATCAAAATGTGACCG+AGG | 0.683966 | 8.3:-48574214 | None:intergenic |
| TTGAGACTGAAAACTACGCG+TGG | 0.689195 | 8.3:-48569223 | None:intergenic |
| GTGAACCAACACTATGAAGA+GGG | 0.703447 | 8.3:+48569288 | MS.gene32257:CDS |
| CTGTTATTGAGGCACAACTG+AGG | 0.710988 | 8.3:+48573187 | MS.gene32257:CDS |
| ATGGCAGGGAATAAATACAA+CGG | 0.717238 | 8.3:+48566181 | MS.gene32257:CDS |
| TGTGAACCAACACTATGAAG+AGG | 0.724349 | 8.3:+48569287 | MS.gene32257:CDS |
| ACTTCACAGAAAGAACACCT+CGG | 0.770222 | 8.3:+48574197 | MS.gene32257:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 48566181 | 48576246 | 48566181 | ID=MS.gene32257 |
| chr8.3 | mRNA | 48566181 | 48576246 | 48566181 | ID=MS.gene32257.t1;Parent=MS.gene32257 |
| chr8.3 | exon | 48566181 | 48566541 | 48566181 | ID=MS.gene32257.t1.exon1;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48566181 | 48566541 | 48566181 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48568747 | 48568844 | 48568747 | ID=MS.gene32257.t1.exon2;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48568747 | 48568844 | 48568747 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48569102 | 48569490 | 48569102 | ID=MS.gene32257.t1.exon3;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48569102 | 48569490 | 48569102 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48569567 | 48569681 | 48569567 | ID=MS.gene32257.t1.exon4;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48569567 | 48569681 | 48569567 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48569850 | 48569951 | 48569850 | ID=MS.gene32257.t1.exon5;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48569850 | 48569951 | 48569850 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48570071 | 48570221 | 48570071 | ID=MS.gene32257.t1.exon6;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48570071 | 48570221 | 48570071 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48570475 | 48570563 | 48570475 | ID=MS.gene32257.t1.exon7;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48570475 | 48570563 | 48570475 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48571037 | 48571085 | 48571037 | ID=MS.gene32257.t1.exon8;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48571037 | 48571085 | 48571037 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48571342 | 48571442 | 48571342 | ID=MS.gene32257.t1.exon9;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48571342 | 48571442 | 48571342 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48572011 | 48572198 | 48572011 | ID=MS.gene32257.t1.exon10;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48572011 | 48572198 | 48572011 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48573173 | 48573290 | 48573173 | ID=MS.gene32257.t1.exon11;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48573173 | 48573290 | 48573173 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48573726 | 48573893 | 48573726 | ID=MS.gene32257.t1.exon12;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48573726 | 48573893 | 48573726 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48573975 | 48574097 | 48573975 | ID=MS.gene32257.t1.exon13;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48573975 | 48574097 | 48573975 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48574184 | 48574277 | 48574184 | ID=MS.gene32257.t1.exon14;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48574184 | 48574277 | 48574184 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48574367 | 48574497 | 48574367 | ID=MS.gene32257.t1.exon15;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48574367 | 48574497 | 48574367 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48574731 | 48574829 | 48574731 | ID=MS.gene32257.t1.exon16;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48574731 | 48574829 | 48574731 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48575418 | 48575753 | 48575418 | ID=MS.gene32257.t1.exon17;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48575418 | 48575753 | 48575418 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| chr8.3 | exon | 48576025 | 48576246 | 48576025 | ID=MS.gene32257.t1.exon18;Parent=MS.gene32257.t1 |
| chr8.3 | CDS | 48576025 | 48576246 | 48576025 | ID=cds.MS.gene32257.t1;Parent=MS.gene32257.t1 |
| Gene Sequence |
| Protein sequence |
