AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32658

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32658.t1 MTR_3g106690 97.297 333 9 0 1 333 59 391 0.0 677
MS.gene32658.t1 MTR_7g103260 58.559 333 135 2 1 333 68 397 1.92e-149 426
MS.gene32658.t1 MTR_1g061760 57.784 334 137 3 1 333 154 484 5.27e-144 416
MS.gene32658.t1 MTR_7g103270 59.104 335 130 4 1 333 69 398 5.73e-144 412
MS.gene32658.t1 MTR_3g080690 58.006 331 136 2 1 331 115 442 1.55e-142 410
MS.gene32658.t1 MTR_3g081240 58.308 331 135 2 1 331 117 444 2.02e-142 410
MS.gene32658.t1 MTR_3g097310 58.559 333 135 2 1 333 162 491 1.13e-140 407
MS.gene32658.t1 MTR_4g115530 53.846 338 149 3 1 333 55 390 5.27e-130 377
MS.gene32658.t1 MTR_4g115530 53.846 338 149 3 1 333 1 336 6.43e-130 375
MS.gene32658.t1 MTR_4g115540 54.142 338 148 3 1 333 289 624 2.05e-128 380
MS.gene32658.t1 MTR_4g115460 52.976 336 153 3 1 333 129 462 4.43e-124 364
MS.gene32658.t1 MTR_4g100730 47.619 336 168 5 1 330 200 533 2.61e-102 311
MS.gene32658.t1 MTR_3g110198 46.386 332 170 4 1 329 264 590 2.06e-97 300
MS.gene32658.t1 MTR_7g103150 45.482 332 173 5 1 329 155 481 2.83e-97 296
MS.gene32658.t1 MTR_3g110200 44.578 332 176 5 1 329 261 587 3.75e-96 296
MS.gene32658.t1 MTR_1g006600 45.783 332 172 5 1 329 257 583 1.73e-94 292
MS.gene32658.t1 MTR_8g102840 42.342 333 183 4 1 329 279 606 1.83e-90 283
MS.gene32658.t1 MTR_1g090733 43.413 334 175 6 1 330 222 545 6.26e-90 279
MS.gene32658.t1 MTR_4g115540 53.629 248 108 3 1 243 289 534 1.57e-89 278
MS.gene32658.t1 MTR_5g031480 39.706 340 182 8 1 332 90 414 1.61e-76 241
MS.gene32658.t1 MTR_5g049790 39.466 337 171 9 1 332 126 434 9.44e-73 231
MS.gene32658.t1 MTR_7g085840 39.706 340 181 11 1 331 1 325 3.29e-64 206
MS.gene32658.t1 MTR_8g102840 39.919 248 140 4 1 244 279 521 1.33e-57 195
MS.gene32658.t1 MTR_5g031480 39.062 256 133 8 1 248 90 330 2.74e-48 165
MS.gene32658.t1 MTR_5g031480 38.824 255 133 8 1 247 90 329 1.32e-47 164
MS.gene32658.t1 MTR_5g049790 39.437 213 109 7 1 208 126 323 9.83e-37 134
MS.gene32658.t1 MTR_5g049790 39.437 213 109 7 1 208 143 340 1.03e-36 135
MS.gene32658.t1 MTR_8g096140 28.873 284 175 7 67 324 102 384 1.73e-28 114
MS.gene32658.t1 MTR_8g096160 29.577 284 173 8 67 324 102 384 2.63e-28 114
MS.gene32658.t1 MTR_2g084245 28.000 300 192 7 45 324 131 426 1.65e-24 103
MS.gene32658.t1 MTR_4g057500 27.869 305 186 9 45 324 149 444 4.78e-23 99.8
MS.gene32658.t1 MTR_8g080490 25.714 315 204 13 27 324 97 398 4.72e-17 82.0
MS.gene32658.t1 MTR_1g029350 24.924 329 206 9 2 302 71 386 7.24e-16 78.6
MS.gene32658.t1 MTR_3g037720 25.773 291 198 8 45 324 187 470 1.08e-15 78.2
MS.gene32658.t1 MTR_4g084200 26.582 316 178 14 45 333 160 448 2.22e-15 77.0
MS.gene32658.t1 MTR_6g027310 26.603 312 188 13 45 334 183 475 6.40e-15 75.9
MS.gene32658.t1 MTR_3g085480 26.936 297 188 13 45 324 116 400 2.70e-14 73.6
MS.gene32658.t1 MTR_4g077920 25.079 315 192 9 49 333 111 411 8.82e-14 72.0
MS.gene32658.t1 MTR_4g084200 28.521 284 153 14 45 297 160 424 3.25e-13 70.5
MS.gene32658.t1 MTR_4g084200 27.559 254 141 10 45 274 160 394 7.10e-13 69.3
MS.gene32658.t1 MTR_4g077920 25.954 262 157 8 95 333 222 469 1.07e-11 65.9
MS.gene32658.t1 MTR_7g087100 24.055 291 202 7 45 324 188 470 1.10e-11 65.9
MS.gene32658.t1 MTR_4g077920 27.350 234 147 8 95 312 222 448 4.16e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32658.t1 AT5G25310 67.365 334 107 2 1 333 147 479 1.46e-168 478
MS.gene32658.t1 AT5G03795 58.084 334 137 2 1 334 186 516 7.40e-144 416
MS.gene32658.t1 AT3G07620 57.658 333 139 1 1 333 128 458 5.76e-137 397
MS.gene32658.t1 AT5G11130 51.796 334 158 2 1 333 1 332 6.14e-133 382
MS.gene32658.t1 AT5G11130 51.796 334 158 2 1 333 145 476 1.38e-131 384
MS.gene32658.t1 AT5G20260 52.695 334 156 2 1 333 1 333 1.27e-125 363
MS.gene32658.t1 AT5G20260 52.695 334 156 2 1 333 28 360 1.51e-125 364
MS.gene32658.t1 AT5G20260 52.695 334 156 2 1 333 133 465 7.14e-125 366
MS.gene32658.t1 AT3G42180 50.147 341 160 3 1 333 1 339 4.80e-122 354
MS.gene32658.t1 AT5G33290 51.613 341 155 3 1 333 160 498 3.60e-121 358
MS.gene32658.t1 AT3G42180 50.147 341 160 3 1 333 130 468 4.59e-121 357
MS.gene32658.t1 AT4G16745 48.657 335 166 3 1 330 196 529 8.35e-111 333
MS.gene32658.t1 AT5G19670 45.946 333 171 4 1 329 277 604 1.59e-100 308
MS.gene32658.t1 AT5G19670 45.946 333 171 4 1 329 277 604 1.59e-100 308
MS.gene32658.t1 AT4G16745 48.387 310 154 3 1 305 196 504 5.61e-100 306
MS.gene32658.t1 AT4G32790 45.345 333 173 5 1 329 263 590 9.75e-96 296
MS.gene32658.t1 AT4G32790 45.345 333 173 5 1 329 263 590 9.75e-96 296
MS.gene32658.t1 AT4G32790 45.345 333 173 5 1 329 263 590 9.75e-96 296
MS.gene32658.t1 AT5G25820 44.012 334 178 5 1 329 320 649 7.08e-91 285
MS.gene32658.t1 AT5G37000 41.246 337 180 6 1 329 222 548 3.82e-84 265
MS.gene32658.t1 AT5G11610 42.090 335 180 10 1 329 216 542 3.24e-80 254
MS.gene32658.t1 AT4G38040 40.000 340 181 8 1 332 100 424 1.10e-74 236
MS.gene32658.t1 AT4G38040 39.550 311 165 8 1 303 100 395 2.16e-63 207
MS.gene32658.t1 AT5G11610 39.834 241 132 9 1 236 216 448 1.76e-47 166
MS.gene32658.t1 AT1G27440 28.956 297 189 8 45 324 89 380 4.55e-28 113
MS.gene32658.t1 AT5G61840 28.378 296 192 7 45 324 92 383 1.13e-27 112
MS.gene32658.t1 AT5G22940 29.126 309 188 10 45 330 152 452 7.52e-27 110
MS.gene32658.t1 AT5G22940 29.126 309 188 10 45 330 181 481 1.05e-26 110
MS.gene32658.t1 AT2G28110 26.496 351 216 9 5 324 94 433 6.06e-26 107
MS.gene32658.t1 AT5G11610 41.739 115 64 2 1 113 216 329 8.64e-25 103
MS.gene32658.t1 AT1G21480 25.083 303 167 12 5 270 89 368 2.70e-15 76.6
MS.gene32658.t1 AT1G21480 25.083 303 167 12 5 270 89 368 4.09e-15 76.3
MS.gene32658.t1 AT1G74680 27.365 296 187 9 45 324 149 432 6.83e-15 75.5
MS.gene32658.t1 AT2G35100 26.280 293 165 13 45 310 127 395 8.07e-15 75.5
MS.gene32658.t1 AT1G67410 25.159 314 211 11 27 324 101 406 1.65e-14 74.3
MS.gene32658.t1 AT5G16890 28.968 252 162 9 95 333 228 475 2.57e-13 70.9
MS.gene32658.t1 AT3G45400 33.058 121 74 2 218 333 327 445 6.91e-12 66.6
MS.gene32658.t1 AT5G44930 25.893 224 138 9 97 305 176 386 9.61e-12 66.2
MS.gene32658.t1 AT5G44930 25.893 224 138 9 97 305 176 386 9.61e-12 66.2

Find 99 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCCATAGAGATGAATTTC+TGG 0.201182 3.1:-84568382 None:intergenic
TCAGAGACAAACTGCGTTAA+AGG 0.217419 3.1:-84567831 None:intergenic
CGGTATCTTGCCTTCTTTGC+CGG 0.242835 3.1:+84568454 MS.gene32658:CDS
GAACTTGGAGCTTAGCATTA+TGG 0.244455 3.1:-84568581 None:intergenic
TAAATTGATTCCACAATTCT+TGG 0.255386 3.1:-84568637 None:intergenic
AAACCCCATGTTCCATTTCA+TGG 0.279328 3.1:-84567701 None:intergenic
TTACCAGAAATTCATCTCTA+TGG 0.286433 3.1:+84568379 MS.gene32658:CDS
GAATAGTAGTCTATGCCTTT+AGG 0.293641 3.1:-84568559 None:intergenic
TCATGCTTGCTTGCCATGAT+TGG 0.306171 3.1:+84567916 MS.gene32658:CDS
TATCAATGAGGTACCCCTTC+TGG 0.307231 3.1:+84567868 MS.gene32658:CDS
GGTAAAGATACATCCTTTAG+AGG 0.312438 3.1:-84568361 None:intergenic
TTCCAGTGTTGGAGAAGAAT+TGG 0.325056 3.1:-84568499 None:intergenic
TGGAGAAGAATTGGGCGAAT+TGG 0.350998 3.1:-84568490 None:intergenic
GTGGATGTTTCAGATATTCC+TGG 0.372580 3.1:+84568745 MS.gene32658:CDS
GGTGGAAGTGAAAGGAGTTT+AGG 0.373477 3.1:-84568415 None:intergenic
TACCGGCGCGGGGCGTTGTC+AGG 0.380651 3.1:-84568436 None:intergenic
GGAGAAGAATTGGGCGAATT+GGG 0.381140 3.1:-84568489 None:intergenic
AATAGTAGTCTATGCCTTTA+GGG 0.390854 3.1:-84568558 None:intergenic
AGAAGCTCAAGCAAGGAGTA+AGG 0.412812 3.1:+84568809 MS.gene32658:CDS
ATGAAATGGAACATGGGGTT+TGG 0.415076 3.1:+84567703 MS.gene32658:CDS
TCCAGTGTTGGAGAAGAATT+GGG 0.427997 3.1:-84568498 None:intergenic
AGCTTCTTATACTCACTCTC+TGG 0.429763 3.1:-84568793 None:intergenic
TGAAGGTTTCAATCCTCTAA+AGG 0.433578 3.1:+84568348 MS.gene32658:CDS
AAATTGATTCCACAATTCTT+GGG 0.433600 3.1:-84568636 None:intergenic
AAAGTAAACATGAGCTGCAT+TGG 0.434974 3.1:-84567745 None:intergenic
AGGAAGGTTCCTCCATGAAA+TGG 0.439602 3.1:+84567689 MS.gene32658:CDS
GCCCAATTCTTCTCCAACAC+TGG 0.442592 3.1:+84568497 MS.gene32658:CDS
CATGCTTGCTTGCCATGATT+GGG 0.450735 3.1:+84567917 MS.gene32658:CDS
ACAATAGGTAGGTCCCCATC+TGG 0.451801 3.1:-84567630 None:intergenic
TAAAGGAGTGACGTCGTAAG+AGG 0.467103 3.1:-84567814 None:intergenic
TGTTCATGATGGCCCGTGTA+AGG 0.468004 3.1:+84567650 MS.gene32658:CDS
CACGGGCCATCATGAACAAT+AGG 0.471146 3.1:-84567645 None:intergenic
TTCTTTGCCGGTGGTATGCA+TGG 0.475267 3.1:+84568466 MS.gene32658:CDS
CCTTCAGTGATGTGCTGCAA+TGG 0.481513 3.1:+84568704 MS.gene32658:CDS
ATGGAGGAACCTTCCTTCGA+TGG 0.484390 3.1:-84567682 None:intergenic
ACTTGGACAAAGACAGAACT+TGG 0.485720 3.1:-84568596 None:intergenic
TGCAATGCCAACACTTCTGA+AGG 0.485933 3.1:+84568331 MS.gene32658:CDS
AAGTAAACATGAGCTGCATT+GGG 0.487410 3.1:-84567744 None:intergenic
TTGAACAAATTACCCAATCA+TGG 0.495105 3.1:-84567929 None:intergenic
TGGATGTTTCAGATATTCCT+GGG 0.495191 3.1:+84568746 MS.gene32658:CDS
ATGAGCTGCATTGGGATCAC+TGG 0.499158 3.1:-84567736 None:intergenic
ACCATCCATGTCACACTGAA+TGG 0.500488 3.1:-84567771 None:intergenic
CTTCAGTGATGTGCTGCAAT+GGG 0.500866 3.1:+84568705 MS.gene32658:CDS
GCAGGGTCCACATGCATCAA+AGG 0.511644 3.1:+84568270 MS.gene32658:intron
AGAAGGCAAGATACCGGCGC+GGG 0.515751 3.1:-84568447 None:intergenic
CGGCGCGGGGCGTTGTCAGG+TGG 0.518380 3.1:-84568433 None:intergenic
ATGCATACCACCGGCAAAGA+AGG 0.518456 3.1:-84568464 None:intergenic
GTCAATGAGTATCTCCCTAA+AGG 0.518478 3.1:+84568544 MS.gene32658:CDS
AAGTTCTGTCTTTGTCCAAG+TGG 0.519604 3.1:+84568598 MS.gene32658:CDS
GAAGCTCAAGCAAGGAGTAA+GGG 0.521382 3.1:+84568810 MS.gene32658:CDS
CGTAAGAGGATGGTGTATAG+AGG 0.521550 3.1:-84567800 None:intergenic
CGGCAAAGAAGGCAAGATAC+CGG 0.524394 3.1:-84568453 None:intergenic
ATTGAAACCTTCAGAAGTGT+TGG 0.527913 3.1:-84568338 None:intergenic
CACCTGACAACGCCCCGCGC+CGG 0.532096 3.1:+84568434 MS.gene32658:CDS
CTAGCCACTTCAAAACCACT+TGG 0.537494 3.1:-84568613 None:intergenic
AAGGTGTATGTATACCCAGA+TGG 0.543645 3.1:+84567615 MS.gene32658:CDS
GGAGATGGAGAAGATATTCA+AGG 0.549467 3.1:+84567596 None:intergenic
CAGGGTCCACATGCATCAAA+GGG 0.550516 3.1:+84568271 MS.gene32658:intron
CGTTGTCAGGTGGAAGTGAA+AGG 0.555053 3.1:-84568423 None:intergenic
CCATTGCAGCACATCACTGA+AGG 0.558494 3.1:-84568704 None:intergenic
TTCCTCCATGAAATGGAACA+TGG 0.562897 3.1:+84567696 MS.gene32658:CDS
CCCTTCTGGAACAGAACTCA+TGG 0.563069 3.1:+84567882 MS.gene32658:CDS
AGGTGTATGTATACCCAGAT+GGG 0.567201 3.1:+84567616 MS.gene32658:CDS
TAATAATTTGATAGAATCAC+AGG 0.575809 3.1:-84568673 None:intergenic
AATTGGGCCATGCATACCAC+CGG 0.576027 3.1:-84568473 None:intergenic
GACCTACCTATTGTTCATGA+TGG 0.577284 3.1:+84567639 MS.gene32658:CDS
AGTGGCTAGCCCAAGAATTG+TGG 0.578684 3.1:+84568627 MS.gene32658:CDS
ATGGAAGTATTGTAGAGGAA+AGG 0.580992 3.1:-84568298 None:intergenic
TCCTCCATGAAATGGAACAT+GGG 0.584529 3.1:+84567697 MS.gene32658:CDS
CCATGAGTTCTGTTCCAGAA+GGG 0.585895 3.1:-84567882 None:intergenic
ACCATGAGTTCTGTTCCAGA+AGG 0.586634 3.1:-84567883 None:intergenic
TTCTACCATTCAGTGTGACA+TGG 0.586958 3.1:+84567766 MS.gene32658:CDS
CCAGAAATTCATCTCTATGG+AGG 0.596875 3.1:+84568382 MS.gene32658:CDS
TATCTTGCCTTCTTTGCCGG+TGG 0.597223 3.1:+84568457 MS.gene32658:CDS
GAGTATAAGAAGCTCAAGCA+AGG 0.597781 3.1:+84568802 MS.gene32658:CDS
CAATAGGTAGGTCCCCATCT+GGG 0.602453 3.1:-84567629 None:intergenic
CATGAGTTCTGTTCCAGAAG+GGG 0.604621 3.1:-84567881 None:intergenic
CTAAGAATCTCTTTCAACCC+AGG 0.612538 3.1:-84568763 None:intergenic
ACCATTCAGTGTGACATGGA+TGG 0.616761 3.1:+84567770 MS.gene32658:CDS
CCTCCATGAAATGGAACATG+GGG 0.617032 3.1:+84567698 MS.gene32658:CDS
CACGAATGGAAGTATTGTAG+AGG 0.617418 3.1:-84568303 None:intergenic
ACATATACTCCATCGAAGGA+AGG 0.623622 3.1:+84567673 MS.gene32658:CDS
GGAGTGACGTCGTAAGAGGA+TGG 0.626224 3.1:-84567810 None:intergenic
CATGGCAAGCAAGCATGAAG+TGG 0.628450 3.1:-84567911 None:intergenic
TGTCTCTGATTATGTGAGAG+TGG 0.630472 3.1:+84567845 MS.gene32658:CDS
ATGGAGTATATGTCCTTACA+CGG 0.631773 3.1:-84567663 None:intergenic
CATTGCAGCACATCACTGAA+GGG 0.633240 3.1:-84568703 None:intergenic
AGCAAGGAGTAAGGGCTGTG+AGG 0.634342 3.1:+84568818 MS.gene32658:CDS
CAGTGATGTGCTGCAATGGG+AGG 0.641289 3.1:+84568708 MS.gene32658:CDS
GGCATTGCACAAGACACGAA+TGG 0.644582 3.1:-84568317 None:intergenic
GAAGGCAAGATACCGGCGCG+GGG 0.652225 3.1:-84568446 None:intergenic
GGATTGCCCTTTGATGCATG+TGG 0.662943 3.1:-84568277 None:intergenic
AAGGACATATACTCCATCGA+AGG 0.665739 3.1:+84567669 MS.gene32658:CDS
AAGAAGGCAAGATACCGGCG+CGG 0.686082 3.1:-84568448 None:intergenic
TGGAGTATATGTCCTTACAC+GGG 0.689264 3.1:-84567662 None:intergenic
CCCCATGTTCCATTTCATGG+AGG 0.689291 3.1:-84567698 None:intergenic
ATGTGAGAGTGGTATCAATG+AGG 0.692550 3.1:+84567856 MS.gene32658:CDS
GGCCATCATGAACAATAGGT+AGG 0.694574 3.1:-84567641 None:intergenic
GGTGTATGTATACCCAGATG+GGG 0.701214 3.1:+84567617 MS.gene32658:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTTAAAGGAAACAAA+AGG - chr3.1:84568250-84568269 None:intergenic 15.0%
!! AACATACAAAAACTATAGAT+TGG - chr3.1:84568081-84568100 None:intergenic 20.0%
!! AAGTGAAACTATTCATAAAA+TGG - chr3.1:84567989-84568008 None:intergenic 20.0%
!! TAATAATTTGATAGAATCAC+AGG - chr3.1:84568676-84568695 None:intergenic 20.0%
! ATTATTTACTTGACCTAAAG+TGG + chr3.1:84568205-84568224 MS.gene32658:intron 25.0%
! GAAACTATTCATAAAATGGA+TGG - chr3.1:84567985-84568004 None:intergenic 25.0%
! GTTTCCTTTAAATATATGCA+GGG + chr3.1:84568253-84568272 MS.gene32658:intron 25.0%
! TGTTTCCTTTAAATATATGC+AGG + chr3.1:84568252-84568271 MS.gene32658:intron 25.0%
! TTACTGTACTAACAAAATGT+TGG - chr3.1:84568104-84568123 None:intergenic 25.0%
!! AAAACATCAAATCTTTTAGC+CGG - chr3.1:84568865-84568884 None:intergenic 25.0%
!! AAATTGATTCCACAATTCTT+GGG - chr3.1:84568639-84568658 None:intergenic 25.0%
!! TAAATTGATTCCACAATTCT+TGG - chr3.1:84568640-84568659 None:intergenic 25.0%
!!! TTTAGTTTTCCCAGTTATTA+GGG + chr3.1:84568049-84568068 MS.gene32658:intron 25.0%
!!! TTTTAGTTTTCCCAGTTATT+AGG + chr3.1:84568048-84568067 MS.gene32658:intron 25.0%
AAAAGGCTTGAAATTCACAT+TGG - chr3.1:84568233-84568252 None:intergenic 30.0%
AAAGGCTTGAAATTCACATT+GGG - chr3.1:84568232-84568251 None:intergenic 30.0%
AACTAAAATGTGGGTAAAGT+TGG - chr3.1:84568036-84568055 None:intergenic 30.0%
AACTGGGAAAACTAAAATGT+GGG - chr3.1:84568045-84568064 None:intergenic 30.0%
AATAGTAGTCTATGCCTTTA+GGG - chr3.1:84568561-84568580 None:intergenic 30.0%
ATAAATGTGAAGTGTGGAAA+AGG - chr3.1:84567958-84567977 None:intergenic 30.0%
TAAATGTGAAGTGTGGAAAA+GGG - chr3.1:84567957-84567976 None:intergenic 30.0%
TAACTGGGAAAACTAAAATG+TGG - chr3.1:84568046-84568065 None:intergenic 30.0%
TTACCAGAAATTCATCTCTA+TGG + chr3.1:84568379-84568398 MS.gene32658:CDS 30.0%
TTGAACAAATTACCCAATCA+TGG - chr3.1:84567932-84567951 None:intergenic 30.0%
! ACATGATTTTGCACTCAATA+TGG + chr3.1:84568887-84568906 MS.gene32658:CDS 30.0%
AAAGTAAACATGAGCTGCAT+TGG - chr3.1:84567748-84567767 None:intergenic 35.0%
AAGTAAACATGAGCTGCATT+GGG - chr3.1:84567747-84567766 None:intergenic 35.0%
ATGGAGTATATGTCCTTACA+CGG - chr3.1:84567666-84567685 None:intergenic 35.0%
GAATAGTAGTCTATGCCTTT+AGG - chr3.1:84568562-84568581 None:intergenic 35.0%
GGAACAATAAATGTGAAGTG+TGG - chr3.1:84567964-84567983 None:intergenic 35.0%
GGTAAAGATACATCCTTTAG+AGG - chr3.1:84568364-84568383 None:intergenic 35.0%
TGAAGGTTTCAATCCTCTAA+AGG + chr3.1:84568348-84568367 MS.gene32658:CDS 35.0%
TGGACCCTGCATATATTTAA+AGG - chr3.1:84568260-84568279 None:intergenic 35.0%
TGGATGTTTCAGATATTCCT+GGG + chr3.1:84568746-84568765 MS.gene32658:CDS 35.0%
TTTCCCAGTTATTAGGGATT+TGG + chr3.1:84568055-84568074 MS.gene32658:intron 35.0%
! ACATTTTACTCTGAATCAGC+CGG + chr3.1:84568843-84568862 MS.gene32658:CDS 35.0%
! ATTGAAACCTTCAGAAGTGT+TGG - chr3.1:84568341-84568360 None:intergenic 35.0%
!! ATGGAAGTATTGTAGAGGAA+AGG - chr3.1:84568301-84568320 None:intergenic 35.0%
AAACCCCATGTTCCATTTCA+TGG - chr3.1:84567704-84567723 None:intergenic 40.0%
AAGGACATATACTCCATCGA+AGG + chr3.1:84567669-84567688 MS.gene32658:CDS 40.0%
AAGGTGTATGTATACCCAGA+TGG + chr3.1:84567615-84567634 MS.gene32658:CDS 40.0%
AAGTTCTGTCTTTGTCCAAG+TGG + chr3.1:84568598-84568617 MS.gene32658:CDS 40.0%
ACATATACTCCATCGAAGGA+AGG + chr3.1:84567673-84567692 MS.gene32658:CDS 40.0%
ACTTGGACAAAGACAGAACT+TGG - chr3.1:84568599-84568618 None:intergenic 40.0%
AGCTTCTTATACTCACTCTC+TGG - chr3.1:84568796-84568815 None:intergenic 40.0%
AGGTGTATGTATACCCAGAT+GGG + chr3.1:84567616-84567635 MS.gene32658:CDS 40.0%
ATGAAATGGAACATGGGGTT+TGG + chr3.1:84567703-84567722 MS.gene32658:CDS 40.0%
ATGTGAGAGTGGTATCAATG+AGG + chr3.1:84567856-84567875 MS.gene32658:CDS 40.0%
ATTCACATTGGGACCACTTT+AGG - chr3.1:84568221-84568240 None:intergenic 40.0%
CCAGAAATTCATCTCTATGG+AGG + chr3.1:84568382-84568401 MS.gene32658:CDS 40.0%
CCTCCATAGAGATGAATTTC+TGG - chr3.1:84568385-84568404 None:intergenic 40.0%
CTAAGAATCTCTTTCAACCC+AGG - chr3.1:84568766-84568785 None:intergenic 40.0%
GAACTTGGAGCTTAGCATTA+TGG - chr3.1:84568584-84568603 None:intergenic 40.0%
GACCTACCTATTGTTCATGA+TGG + chr3.1:84567639-84567658 MS.gene32658:CDS 40.0%
GAGTATAAGAAGCTCAAGCA+AGG + chr3.1:84568802-84568821 MS.gene32658:CDS 40.0%
GTCAATGAGTATCTCCCTAA+AGG + chr3.1:84568544-84568563 MS.gene32658:CDS 40.0%
GTGGATGTTTCAGATATTCC+TGG + chr3.1:84568745-84568764 MS.gene32658:CDS 40.0%
TCAGAGACAAACTGCGTTAA+AGG - chr3.1:84567834-84567853 None:intergenic 40.0%
TCCTCCATGAAATGGAACAT+GGG + chr3.1:84567697-84567716 MS.gene32658:CDS 40.0%
TGGAGTATATGTCCTTACAC+GGG - chr3.1:84567665-84567684 None:intergenic 40.0%
TGGCCAAATCCCTAATAACT+GGG - chr3.1:84568061-84568080 None:intergenic 40.0%
TGTCTCTGATTATGTGAGAG+TGG + chr3.1:84567845-84567864 MS.gene32658:CDS 40.0%
TTCCTCCATGAAATGGAACA+TGG + chr3.1:84567696-84567715 MS.gene32658:CDS 40.0%
TTCTACCATTCAGTGTGACA+TGG + chr3.1:84567766-84567785 MS.gene32658:CDS 40.0%
TTGGCCAAATCCCTAATAAC+TGG - chr3.1:84568062-84568081 None:intergenic 40.0%
! TCCAGTGTTGGAGAAGAATT+GGG - chr3.1:84568501-84568520 None:intergenic 40.0%
! TTCCAGTGTTGGAGAAGAAT+TGG - chr3.1:84568502-84568521 None:intergenic 40.0%
!! CACGAATGGAAGTATTGTAG+AGG - chr3.1:84568306-84568325 None:intergenic 40.0%
!! TGTCTCTGTTTTTCCAGTGT+TGG - chr3.1:84568513-84568532 None:intergenic 40.0%
!!! TTGTCCAAGTGGTTTTGAAG+TGG + chr3.1:84568609-84568628 MS.gene32658:CDS 40.0%
ACCATCCATGTCACACTGAA+TGG - chr3.1:84567774-84567793 None:intergenic 45.0%
ACCATGAGTTCTGTTCCAGA+AGG - chr3.1:84567886-84567905 None:intergenic 45.0%
ACCATTCAGTGTGACATGGA+TGG + chr3.1:84567770-84567789 MS.gene32658:CDS 45.0%
AGAAGCTCAAGCAAGGAGTA+AGG + chr3.1:84568809-84568828 MS.gene32658:CDS 45.0%
AGGAAGGTTCCTCCATGAAA+TGG + chr3.1:84567689-84567708 MS.gene32658:CDS 45.0%
CATGAGTTCTGTTCCAGAAG+GGG - chr3.1:84567884-84567903 None:intergenic 45.0%
CATGCTTGCTTGCCATGATT+GGG + chr3.1:84567917-84567936 MS.gene32658:CDS 45.0%
CATTGCAGCACATCACTGAA+GGG - chr3.1:84568706-84568725 None:intergenic 45.0%
CCATGAGTTCTGTTCCAGAA+GGG - chr3.1:84567885-84567904 None:intergenic 45.0%
CCTCCATGAAATGGAACATG+GGG + chr3.1:84567698-84567717 MS.gene32658:CDS 45.0%
CGTAAGAGGATGGTGTATAG+AGG - chr3.1:84567803-84567822 None:intergenic 45.0%
CTAGCCACTTCAAAACCACT+TGG - chr3.1:84568616-84568635 None:intergenic 45.0%
CTTCAGTGATGTGCTGCAAT+GGG + chr3.1:84568705-84568724 MS.gene32658:CDS 45.0%
GAAGCTCAAGCAAGGAGTAA+GGG + chr3.1:84568810-84568829 MS.gene32658:CDS 45.0%
GGAGAAGAATTGGGCGAATT+GGG - chr3.1:84568492-84568511 None:intergenic 45.0%
GGCCATCATGAACAATAGGT+AGG - chr3.1:84567644-84567663 None:intergenic 45.0%
GGTGGAAGTGAAAGGAGTTT+AGG - chr3.1:84568418-84568437 None:intergenic 45.0%
GGTGTATGTATACCCAGATG+GGG + chr3.1:84567617-84567636 MS.gene32658:CDS 45.0%
TAAAGGAGTGACGTCGTAAG+AGG - chr3.1:84567817-84567836 None:intergenic 45.0%
TCATGCTTGCTTGCCATGAT+TGG + chr3.1:84567916-84567935 MS.gene32658:CDS 45.0%
TGCAATGCCAACACTTCTGA+AGG + chr3.1:84568331-84568350 MS.gene32658:CDS 45.0%
TGGAGAAGAATTGGGCGAAT+TGG - chr3.1:84568493-84568512 None:intergenic 45.0%
! TATCAATGAGGTACCCCTTC+TGG + chr3.1:84567868-84567887 MS.gene32658:CDS 45.0%
AATTGGGCCATGCATACCAC+CGG - chr3.1:84568476-84568495 None:intergenic 50.0%
ACAATAGGTAGGTCCCCATC+TGG - chr3.1:84567633-84567652 None:intergenic 50.0%
ATGAGCTGCATTGGGATCAC+TGG - chr3.1:84567739-84567758 None:intergenic 50.0%
ATGCATACCACCGGCAAAGA+AGG - chr3.1:84568467-84568486 None:intergenic 50.0%
ATGGAGGAACCTTCCTTCGA+TGG - chr3.1:84567685-84567704 None:intergenic 50.0%
CAATAGGTAGGTCCCCATCT+GGG - chr3.1:84567632-84567651 None:intergenic 50.0%
CACGGGCCATCATGAACAAT+AGG - chr3.1:84567648-84567667 None:intergenic 50.0%
CAGGGTCCACATGCATCAAA+GGG + chr3.1:84568271-84568290 MS.gene32658:intron 50.0%
CATGGCAAGCAAGCATGAAG+TGG - chr3.1:84567914-84567933 None:intergenic 50.0%
CCATTGCAGCACATCACTGA+AGG - chr3.1:84568707-84568726 None:intergenic 50.0%
CCCCATGTTCCATTTCATGG+AGG - chr3.1:84567701-84567720 None:intergenic 50.0%
CCTTCAGTGATGTGCTGCAA+TGG + chr3.1:84568704-84568723 MS.gene32658:CDS 50.0%
CGGCAAAGAAGGCAAGATAC+CGG - chr3.1:84568456-84568475 None:intergenic 50.0%
CGGTATCTTGCCTTCTTTGC+CGG + chr3.1:84568454-84568473 MS.gene32658:CDS 50.0%
CGTTGTCAGGTGGAAGTGAA+AGG - chr3.1:84568426-84568445 None:intergenic 50.0%
GCCCAATTCTTCTCCAACAC+TGG + chr3.1:84568497-84568516 MS.gene32658:CDS 50.0%
GGATTGCCCTTTGATGCATG+TGG - chr3.1:84568280-84568299 None:intergenic 50.0%
GGCATTGCACAAGACACGAA+TGG - chr3.1:84568320-84568339 None:intergenic 50.0%
TATCTTGCCTTCTTTGCCGG+TGG + chr3.1:84568457-84568476 MS.gene32658:CDS 50.0%
TGTTCATGATGGCCCGTGTA+AGG + chr3.1:84567650-84567669 MS.gene32658:CDS 50.0%
TTCTTTGCCGGTGGTATGCA+TGG + chr3.1:84568466-84568485 MS.gene32658:CDS 50.0%
! AGTGGCTAGCCCAAGAATTG+TGG + chr3.1:84568627-84568646 MS.gene32658:CDS 50.0%
!! CCCTTCTGGAACAGAACTCA+TGG + chr3.1:84567882-84567901 MS.gene32658:CDS 50.0%
!!! ATGGGAGGCATTTTCAGTGC+AGG + chr3.1:84568723-84568742 MS.gene32658:CDS 50.0%
AAGAAGGCAAGATACCGGCG+CGG - chr3.1:84568451-84568470 None:intergenic 55.0%
AGCAAGGAGTAAGGGCTGTG+AGG + chr3.1:84568818-84568837 MS.gene32658:CDS 55.0%
CAGTGATGTGCTGCAATGGG+AGG + chr3.1:84568708-84568727 MS.gene32658:CDS 55.0%
GCAGGGTCCACATGCATCAA+AGG + chr3.1:84568270-84568289 MS.gene32658:intron 55.0%
GGAGTGACGTCGTAAGAGGA+TGG - chr3.1:84567813-84567832 None:intergenic 55.0%
!!! GGAGGCATTTTCAGTGCAGG+TGG + chr3.1:84568726-84568745 MS.gene32658:CDS 55.0%
AGAAGGCAAGATACCGGCGC+GGG - chr3.1:84568450-84568469 None:intergenic 60.0%
GAAGGCAAGATACCGGCGCG+GGG - chr3.1:84568449-84568468 None:intergenic 65.0%
CACCTGACAACGCCCCGCGC+CGG + chr3.1:84568434-84568453 MS.gene32658:CDS 75.0%
!! TACCGGCGCGGGGCGTTGTC+AGG - chr3.1:84568439-84568458 None:intergenic 75.0%
!!! CGGCGCGGGGCGTTGTCAGG+TGG - chr3.1:84568436-84568455 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 84567600 84568939 84567600 ID=MS.gene32658
chr3.1 mRNA 84567600 84568939 84567600 ID=MS.gene32658.t1;Parent=MS.gene32658
chr3.1 exon 84567600 84567938 84567600 ID=MS.gene32658.t1.exon1;Parent=MS.gene32658.t1
chr3.1 CDS 84567600 84567938 84567600 ID=cds.MS.gene32658.t1;Parent=MS.gene32658.t1
chr3.1 exon 84568274 84568939 84568274 ID=MS.gene32658.t1.exon2;Parent=MS.gene32658.t1
chr3.1 CDS 84568274 84568939 84568274 ID=cds.MS.gene32658.t1;Parent=MS.gene32658.t1
Gene Sequence

>MS.gene32658

ATGGAGAAGATATTCAAGGTGTATGTATACCCAGATGGGGACCTACCTATTGTTCATGATGGCCCGTGTAAGGACATATACTCCATCGAAGGAAGGTTCCTCCATGAAATGGAACATGGGGTTTGGAAGTTTAGAACCAGTGATCCCAATGCAGCTCATGTTTACTTTCTACCATTCAGTGTGACATGGATGGTCAAATACCTCTATACACCATCCTCTTACGACGTCACTCCTTTAACGCAGTTTGTCTCTGATTATGTGAGAGTGGTATCAATGAGGTACCCCTTCTGGAACAGAACTCATGGTGCTGACCACTTCATGCTTGCTTGCCATGATTGGGTAATTTGTTCAATTCCCTTTTCCACACTTCACATTTATTGTTCCATCCATTTTATGAATAGTTTCACTTTTTGTTAATGACACACAATTGATGCCAACTTTACCCACATTTTAGTTTTCCCAGTTATTAGGGATTTGGCCAATCTATAGTTTTTGTATGTTCCAACATTTTGTTAGTACAGTAAAAAAATTAATAGTTGATATTGTAACATTTGTTATGCATATACATTTAATATCAATTATTGATTTATTTATCAAACTTTTATATTATTTACTTGACCTAAAGTGGTCCCAATGTGAATTTCAAGCCTTTTGTTTCCTTTAAATATATGCAGGGTCCACATGCATCAAAGGGCAATCCTTTCCTCTACAATACTTCCATTCGTGTCTTGTGCAATGCCAACACTTCTGAAGGTTTCAATCCTCTAAAGGATGTATCTTTACCAGAAATTCATCTCTATGGAGGTGAAGTATCTCCTAAACTCCTTTCACTTCCACCTGACAACGCCCCGCGCCGGTATCTTGCCTTCTTTGCCGGTGGTATGCATGGCCCAATTCGCCCAATTCTTCTCCAACACTGGAAAAACAGAGACAAAGATATTATTGTCAATGAGTATCTCCCTAAAGGCATAGACTACTATTCCATAATGCTAAGCTCCAAGTTCTGTCTTTGTCCAAGTGGTTTTGAAGTGGCTAGCCCAAGAATTGTGGAATCAATTTATGCAGAATGTGTACCTGTGATTCTATCAAATTATTATGTGTTGCCCTTCAGTGATGTGCTGCAATGGGAGGCATTTTCAGTGCAGGTGGATGTTTCAGATATTCCTGGGTTGAAAGAGATTCTTAGTGCTATTCCAGAGAGTGAGTATAAGAAGCTCAAGCAAGGAGTAAGGGCTGTGAGGAGACATTTTACTCTGAATCAGCCGGCTAAAAGATTTGATGTTTTTCACATGATTTTGCACTCAATATGGCTAAGAAGATTGAATATAAAACTTGAATAG
Protein sequence

>MS.gene32658.t1

MEKIFKVYVYPDGDLPIVHDGPCKDIYSIEGRFLHEMEHGVWKFRTSDPNAAHVYFLPFSVTWMVKYLYTPSSYDVTPLTQFVSDYVRVVSMRYPFWNRTHGADHFMLACHDWGPHASKGNPFLYNTSIRVLCNANTSEGFNPLKDVSLPEIHLYGGEVSPKLLSLPPDNAPRRYLAFFAGGMHGPIRPILLQHWKNRDKDIIVNEYLPKGIDYYSIMLSSKFCLCPSGFEVASPRIVESIYAECVPVILSNYYVLPFSDVLQWEAFSVQVDVSDIPGLKEILSAIPESEYKKLKQGVRAVRRHFTLNQPAKRFDVFHMILHSIWLRRLNIKLE