Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33033.t1 | ACJ83839.1 | 98.3 | 173 | 3 | 0 | 1 | 173 | 76 | 248 | 8.70E-65 | 256.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33033.t1 | G7I932 | 98.3 | 173 | 3 | 0 | 1 | 173 | 123 | 295 | 6.2e-65 | 256.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049678 | MS.gene33033 | 0.829112 | 6.18E-55 | -1.69E-46 |
| MS.gene05031 | MS.gene33033 | 0.84667 | 1.88E-59 | -1.69E-46 |
| MS.gene050463 | MS.gene33033 | 0.837682 | 4.50E-57 | -1.69E-46 |
| MS.gene050528 | MS.gene33033 | 0.840392 | 8.94E-58 | -1.69E-46 |
| MS.gene051569 | MS.gene33033 | 0.994962 | 9.36E-212 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33033.t1 | MTR_1g015650 | 98.266 | 173 | 3 | 0 | 1 | 173 | 123 | 295 | 7.27e-122 | 345 |
| MS.gene33033.t1 | MTR_3g105210 | 71.277 | 94 | 27 | 0 | 1 | 94 | 59 | 152 | 1.12e-44 | 146 |
| MS.gene33033.t1 | MTR_4g125330 | 41.358 | 162 | 76 | 7 | 1 | 152 | 71 | 223 | 4.81e-22 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33033.t1 | AT5G21940 | 53.465 | 101 | 36 | 4 | 1 | 95 | 78 | 173 | 2.92e-23 | 93.2 |
| MS.gene33033.t1 | AT3G43850 | 49.451 | 91 | 31 | 3 | 1 | 91 | 55 | 130 | 2.28e-18 | 78.6 |
| MS.gene33033.t1 | AT3G43850 | 49.451 | 91 | 31 | 3 | 1 | 91 | 55 | 130 | 5.47e-18 | 79.0 |
Find 27 sgRNAs with CRISPR-Local
Find 84 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAAGAGGTTCTTCCTATT+AGG | 0.287448 | 1.1:+6300823 | MS.gene33033:CDS |
| GCCACTTCCACCGCGTCATC+CGG | 0.302841 | 1.1:+6302216 | MS.gene33033:CDS |
| ATAAGGAATGGTTACCTAAT+AGG | 0.349508 | 1.1:-6300837 | None:intergenic |
| CTTCTTGTGTATGCATTCTC+TGG | 0.380516 | 1.1:-6301983 | None:intergenic |
| AATTTGCTGGCTTTCAATCA+TGG | 0.423862 | 1.1:+6302013 | MS.gene33033:CDS |
| AAGAAGACGTAGAAATTTGC+TGG | 0.426140 | 1.1:+6302000 | MS.gene33033:CDS |
| GCTGGCTTTCAATCATGGTT+GGG | 0.432602 | 1.1:+6302018 | MS.gene33033:CDS |
| AGTATCTCAAACTTCTATAG+TGG | 0.435354 | 1.1:+6301893 | MS.gene33033:CDS |
| AAAGAAGACACTCTATTTCC+CGG | 0.439102 | 1.1:-6302235 | None:intergenic |
| CGCGGTGGAAGTGGCGAAGA+AGG | 0.445270 | 1.1:-6302208 | None:intergenic |
| TGCTGGCTTTCAATCATGGT+TGG | 0.445425 | 1.1:+6302017 | MS.gene33033:CDS |
| TTCCCTTTGAGAAGCAATAG+TGG | 0.449201 | 1.1:+6302055 | MS.gene33033:CDS |
| CCCGGATGACGCGGTGGAAG+TGG | 0.453467 | 1.1:-6302217 | None:intergenic |
| AGCTAGGCTTGTGAAGGACT+TGG | 0.454561 | 1.1:-6301921 | None:intergenic |
| CACCACTATTGCTTCTCAAA+GGG | 0.472099 | 1.1:-6302057 | None:intergenic |
| CCACCACTATTGCTTCTCAA+AGG | 0.509782 | 1.1:-6302058 | None:intergenic |
| CCACTTCCACCGCGTCATCC+GGG | 0.531769 | 1.1:+6302217 | MS.gene33033:CDS |
| AGAAGGTGCTGAAGAATATG+AGG | 0.542161 | 1.1:-6302191 | None:intergenic |
| GAAAAGAAATTCCTCTGCAA+AGG | 0.546535 | 1.1:-6302265 | None:intergenic |
| CTTTAGCATCTCCTTTGCAG+AGG | 0.570257 | 1.1:+6302254 | MS.gene33033:CDS |
| CCTTTGAGAAGCAATAGTGG+TGG | 0.600401 | 1.1:+6302058 | MS.gene33033:CDS |
| TTGCATGGAAGCTTTAGAAG+AGG | 0.606872 | 1.1:+6300809 | None:intergenic |
| CTATTTCCCGGATGACGCGG+TGG | 0.613883 | 1.1:-6302223 | None:intergenic |
| ACAATGATGCAGATCAGCCA+AGG | 0.617708 | 1.1:-6302287 | None:intergenic |
| AACATCAGCTAGGCTTGTGA+AGG | 0.619105 | 1.1:-6301927 | None:intergenic |
| GTGTTGTGACAACATCAGCT+AGG | 0.640480 | 1.1:-6301937 | None:intergenic |
| ACTCTATTTCCCGGATGACG+CGG | 0.665825 | 1.1:-6302226 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTATATCAAAATTTACTA+TGG | + | chr1.1:6301765-6301784 | MS.gene33033:intron | 10.0% |
| !!! | AAAGATTGTATATTTTTTTT+TGG | + | chr1.1:6301092-6301111 | MS.gene33033:intron | 10.0% |
| !!! | AAGATTGTATATTTTTTTTT+GGG | + | chr1.1:6301093-6301112 | MS.gene33033:intron | 10.0% |
| !! | GGTATTAGTTAAATTAATTA+GGG | + | chr1.1:6301164-6301183 | MS.gene33033:intron | 15.0% |
| !!! | AATATTGCCTTTTTTATTTT+TGG | + | chr1.1:6301559-6301578 | MS.gene33033:intron | 15.0% |
| !!! | TTTTTTAAATGTTTTAAGCA+GGG | + | chr1.1:6301057-6301076 | MS.gene33033:intron | 15.0% |
| !!! | TTTTTTTAAATGTTTTAAGC+AGG | + | chr1.1:6301056-6301075 | MS.gene33033:intron | 15.0% |
| !! | AAAAGTTTGTGAATGAAATT+TGG | + | chr1.1:6301216-6301235 | MS.gene33033:intron | 20.0% |
| !! | GGGTATTAGTTAAATTAATT+AGG | + | chr1.1:6301163-6301182 | MS.gene33033:intron | 20.0% |
| !! | TAATGGAAAAACTGTTAATA+AGG | - | chr1.1:6300857-6300876 | None:intergenic | 20.0% |
| !! | TGATATAAATCTACACAATT+TGG | - | chr1.1:6301755-6301774 | None:intergenic | 20.0% |
| !!! | AATTTTGCTAAAAATAGCAA+GGG | + | chr1.1:6301366-6301385 | MS.gene33033:intron | 20.0% |
| !!! | ATTAGTGTTCTAAATAATGA+AGG | + | chr1.1:6301839-6301858 | MS.gene33033:intron | 20.0% |
| !!! | TCATTTTCAATTGAAGAATT+TGG | - | chr1.1:6302337-6302356 | None:intergenic | 20.0% |
| ! | AACAAAACATTCAAAAGTGA+AGG | - | chr1.1:6300942-6300961 | None:intergenic | 25.0% |
| ! | ATCCAATTCAAAAGTTTATC+CGG | - | chr1.1:6301524-6301543 | None:intergenic | 25.0% |
| ! | GAAAAACTGTTAATAAGGAA+TGG | - | chr1.1:6300852-6300871 | None:intergenic | 25.0% |
| ! | TCCTCAACCAAAAATAAAAA+AGG | - | chr1.1:6301569-6301588 | None:intergenic | 25.0% |
| ! | TGTTTCAGTTTCACAAAATT+AGG | + | chr1.1:6301324-6301343 | MS.gene33033:intron | 25.0% |
| !! | AAGGCTAATCCAATATTTTA+TGG | + | chr1.1:6301858-6301877 | MS.gene33033:intron | 25.0% |
| !! | AATTGTTGGATCAATTTTTG+TGG | + | chr1.1:6301141-6301160 | MS.gene33033:intron | 25.0% |
| !! | AATTTGGTGATTGTATTGTA+AGG | + | chr1.1:6301232-6301251 | MS.gene33033:intron | 25.0% |
| !! | ATTAATTAGGGTACTATTGT+TGG | + | chr1.1:6301176-6301195 | MS.gene33033:intron | 25.0% |
| !! | ATTGTTGGATCAATTTTTGT+GGG | + | chr1.1:6301142-6301161 | MS.gene33033:intron | 25.0% |
| !! | ATTTTGTGAAACTGAAACAA+AGG | - | chr1.1:6301323-6301342 | None:intergenic | 25.0% |
| !! | CTTTTGATAGAAGAAAACTT+GGG | + | chr1.1:6301465-6301484 | MS.gene33033:intron | 25.0% |
| !! | GAATTTTGCTAAAAATAGCA+AGG | + | chr1.1:6301365-6301384 | MS.gene33033:intron | 25.0% |
| !! | GGTATTAGTTAAATTAGCTT+GGG | + | chr1.1:6301114-6301133 | MS.gene33033:intron | 25.0% |
| !! | TTTACAATGTCTTTTACAGA+TGG | - | chr1.1:6301962-6301981 | None:intergenic | 25.0% |
| !! | TTTTGTGAAACTGAAACAAA+GGG | - | chr1.1:6301322-6301341 | None:intergenic | 25.0% |
| !!! | ATTTTTAGCAAAATTCCACA+TGG | - | chr1.1:6301361-6301380 | None:intergenic | 25.0% |
| AGTATCTCAAACTTCTATAG+TGG | + | chr1.1:6301893-6301912 | MS.gene33033:CDS | 30.0% | |
| ATAAGGAATGGTTACCTAAT+AGG | - | chr1.1:6300840-6300859 | None:intergenic | 30.0% | |
| CCTGCATAACCATAAAATAT+TGG | - | chr1.1:6301870-6301889 | None:intergenic | 30.0% | |
| CTAAAGAACATGTTTGCAAT+TGG | + | chr1.1:6301494-6301513 | MS.gene33033:intron | 30.0% | |
| GGGTATTAGTTAAATTAGCT+TGG | + | chr1.1:6301113-6301132 | MS.gene33033:intron | 30.0% | |
| GTTTGAATCAACTTTCATTC+TGG | - | chr1.1:6301726-6301745 | None:intergenic | 30.0% | |
| ! | CCAATATTTTATGGTTATGC+AGG | + | chr1.1:6301867-6301886 | MS.gene33033:intron | 30.0% |
| ! | GCTTTTGATAGAAGAAAACT+TGG | + | chr1.1:6301464-6301483 | MS.gene33033:intron | 30.0% |
| ! | TAATTCATGTTTGCCTTGTT+TGG | + | chr1.1:6301597-6301616 | MS.gene33033:intron | 30.0% |
| ! | TACCGGATAAACTTTTGAAT+TGG | + | chr1.1:6301519-6301538 | MS.gene33033:intron | 30.0% |
| ! | TTGTTGGATCAATTTTTGTG+GGG | + | chr1.1:6301143-6301162 | MS.gene33033:intron | 30.0% |
| !!! | GCCTTTTTTATTTTTGGTTG+AGG | + | chr1.1:6301565-6301584 | MS.gene33033:intron | 30.0% |
| !!! | TAGGTTTATTTTGTACCATG+TGG | + | chr1.1:6301343-6301362 | MS.gene33033:intron | 30.0% |
| AAAGAAGACACTCTATTTCC+CGG | - | chr1.1:6302238-6302257 | None:intergenic | 35.0% | |
| AAGAAGACGTAGAAATTTGC+TGG | + | chr1.1:6302000-6302019 | MS.gene33033:CDS | 35.0% | |
| CAACGTGATTGTATAGTTTC+GGG | + | chr1.1:6301662-6301681 | MS.gene33033:intron | 35.0% | |
| CCAAAACATGACGAATTCAT+TGG | - | chr1.1:6301418-6301437 | None:intergenic | 35.0% | |
| GAAAAGAAATTCCTCTGCAA+AGG | - | chr1.1:6302268-6302287 | None:intergenic | 35.0% | |
| GCAACGTGATTGTATAGTTT+CGG | + | chr1.1:6301661-6301680 | MS.gene33033:intron | 35.0% | |
| GCATGATTGAAGCTTACAAA+AGG | - | chr1.1:6301446-6301465 | None:intergenic | 35.0% | |
| TAGAAGAGGTTCTTCCTATT+AGG | + | chr1.1:6300823-6300842 | MS.gene33033:CDS | 35.0% | |
| TGTTTGGTACTTGTTTGCAA+TGG | + | chr1.1:6301613-6301632 | MS.gene33033:intron | 35.0% | |
| TTGCAAACAAGTACCAAACA+AGG | - | chr1.1:6301613-6301632 | None:intergenic | 35.0% | |
| ! | CATTTTCATTGTTGCAGCAA+TGG | - | chr1.1:6302314-6302333 | None:intergenic | 35.0% |
| ! | CCAATGAATTCGTCATGTTT+TGG | + | chr1.1:6301415-6301434 | MS.gene33033:intron | 35.0% |
| ! | TGTGTGATGATGTGATCTAT+AGG | + | chr1.1:6301276-6301295 | MS.gene33033:intron | 35.0% |
| ! | TTAGCTTGGGTACTAATTGT+TGG | + | chr1.1:6301127-6301146 | MS.gene33033:intron | 35.0% |
| !! | AATTTGCTGGCTTTCAATCA+TGG | + | chr1.1:6302013-6302032 | MS.gene33033:CDS | 35.0% |
| ATCACATCATCACACACTAC+TGG | - | chr1.1:6301272-6301291 | None:intergenic | 40.0% | |
| CACCACTATTGCTTCTCAAA+GGG | - | chr1.1:6302060-6302079 | None:intergenic | 40.0% | |
| CATGTTTGCAATTGGTGTAC+CGG | + | chr1.1:6301502-6301521 | MS.gene33033:intron | 40.0% | |
| CTGTGAGTTTGACGATATCA+TGG | + | chr1.1:6301636-6301655 | MS.gene33033:intron | 40.0% | |
| CTTCTTGTGTATGCATTCTC+TGG | - | chr1.1:6301986-6302005 | None:intergenic | 40.0% | |
| TGAGTTTGACGATATCATGG+TGG | + | chr1.1:6301639-6301658 | MS.gene33033:intron | 40.0% | |
| ! | GCAGAGGAATTTCTTTTCCT+TGG | + | chr1.1:6302270-6302289 | MS.gene33033:CDS | 40.0% |
| ! | TTCCCTTTGAGAAGCAATAG+TGG | + | chr1.1:6302055-6302074 | MS.gene33033:CDS | 40.0% |
| !! | AGAAGGTGCTGAAGAATATG+AGG | - | chr1.1:6302194-6302213 | None:intergenic | 40.0% |
| AACATCAGCTAGGCTTGTGA+AGG | - | chr1.1:6301930-6301949 | None:intergenic | 45.0% | |
| ACAATGATGCAGATCAGCCA+AGG | - | chr1.1:6302290-6302309 | None:intergenic | 45.0% | |
| CCACCACTATTGCTTCTCAA+AGG | - | chr1.1:6302061-6302080 | None:intergenic | 45.0% | |
| CTTTAGCATCTCCTTTGCAG+AGG | + | chr1.1:6302254-6302273 | MS.gene33033:CDS | 45.0% | |
| GTGTTGTGACAACATCAGCT+AGG | - | chr1.1:6301940-6301959 | None:intergenic | 45.0% | |
| ! | CCTTTGAGAAGCAATAGTGG+TGG | + | chr1.1:6302058-6302077 | MS.gene33033:CDS | 45.0% |
| !! | GCTGGCTTTCAATCATGGTT+GGG | + | chr1.1:6302018-6302037 | MS.gene33033:CDS | 45.0% |
| !! | TGCTGGCTTTCAATCATGGT+TGG | + | chr1.1:6302017-6302036 | MS.gene33033:CDS | 45.0% |
| !! | ATATATATACATATATATAA+TGG | - | chr1.1:6300874-6300893 | None:intergenic | 5.0% |
| ACTCTATTTCCCGGATGACG+CGG | - | chr1.1:6302229-6302248 | None:intergenic | 50.0% | |
| AGCTAGGCTTGTGAAGGACT+TGG | - | chr1.1:6301924-6301943 | None:intergenic | 50.0% | |
| CTATTTCCCGGATGACGCGG+TGG | - | chr1.1:6302226-6302245 | None:intergenic | 60.0% | |
| CCACTTCCACCGCGTCATCC+GGG | + | chr1.1:6302217-6302236 | MS.gene33033:CDS | 65.0% | |
| CGCGGTGGAAGTGGCGAAGA+AGG | - | chr1.1:6302211-6302230 | None:intergenic | 65.0% | |
| GCCACTTCCACCGCGTCATC+CGG | + | chr1.1:6302216-6302235 | MS.gene33033:CDS | 65.0% | |
| CCCGGATGACGCGGTGGAAG+TGG | - | chr1.1:6302220-6302239 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 6300813 | 6302378 | 6300813 | ID=MS.gene33033 |
| chr1.1 | mRNA | 6300813 | 6302378 | 6300813 | ID=MS.gene33033.t1;Parent=MS.gene33033 |
| chr1.1 | exon | 6300813 | 6300844 | 6300813 | ID=MS.gene33033.t1.exon1;Parent=MS.gene33033.t1 |
| chr1.1 | CDS | 6300813 | 6300844 | 6300813 | ID=cds.MS.gene33033.t1;Parent=MS.gene33033.t1 |
| chr1.1 | exon | 6301889 | 6302378 | 6301889 | ID=MS.gene33033.t1.exon2;Parent=MS.gene33033.t1 |
| chr1.1 | CDS | 6301889 | 6302378 | 6301889 | ID=cds.MS.gene33033.t1;Parent=MS.gene33033.t1 |
| Gene Sequence |
| Protein sequence |
