AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33185

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33185.t1 MTR_1g012760 92.832 279 18 1 1 279 1 277 0.0 538
MS.gene33185.t1 MTR_1g012760 89.455 275 27 1 5 279 16 288 0.0 508
MS.gene33185.t1 MTR_2g090505 39.773 176 100 2 35 204 83 258 6.93e-38 136
MS.gene33185.t1 MTR_1g099140 44.242 165 86 2 40 198 88 252 1.81e-36 136
MS.gene33185.t1 MTR_6g029530 41.243 177 94 3 30 196 493 669 2.80e-34 131
MS.gene33185.t1 MTR_3g116710 40.678 177 96 4 26 194 67 242 1.11e-33 123
MS.gene33185.t1 MTR_3g077875 35.795 176 106 3 35 204 78 252 4.15e-33 122
MS.gene33185.t1 MTR_3g015530 39.490 157 91 2 32 184 46 202 5.35e-33 121
MS.gene33185.t1 MTR_3g034840 39.490 157 88 3 24 174 57 212 5.78e-31 115
MS.gene33185.t1 MTR_0599s0020 39.490 157 88 3 24 174 57 212 5.78e-31 115
MS.gene33185.t1 MTR_3g072200 39.735 151 87 2 49 195 59 209 3.12e-30 119
MS.gene33185.t1 MTR_6g464570 37.931 145 83 3 29 166 8 152 3.72e-28 106
MS.gene33185.t1 MTR_5g046145 43.165 139 70 2 40 175 124 256 5.40e-28 110
MS.gene33185.t1 MTR_4g119600 38.636 176 93 4 35 201 60 229 2.05e-27 108
MS.gene33185.t1 MTR_5g006100 36.585 164 97 3 47 204 66 228 1.53e-26 108
MS.gene33185.t1 MTR_6g075990 37.356 174 94 4 35 199 78 245 2.78e-26 103
MS.gene33185.t1 MTR_0528s0010 42.105 114 66 0 91 204 6 119 6.26e-26 100
MS.gene33185.t1 MTR_5g046160 38.509 161 75 5 50 204 55 197 4.59e-25 100
MS.gene33185.t1 MTR_5g032220 34.682 173 97 2 35 204 71 230 6.81e-25 103
MS.gene33185.t1 MTR_3g087440 38.028 142 81 3 41 176 84 224 3.79e-24 97.8
MS.gene33185.t1 MTR_5g031650 39.333 150 76 4 35 175 82 225 6.82e-24 97.1
MS.gene33185.t1 MTR_4g120790 32.571 175 89 2 31 204 60 206 7.11e-24 97.1
MS.gene33185.t1 MTR_2g026585 30.178 169 90 2 32 199 60 201 1.04e-21 94.0
MS.gene33185.t1 MTR_1g081740 31.646 158 80 2 32 188 59 189 3.25e-21 89.0
MS.gene33185.t1 MTR_2g461140 33.962 159 72 3 45 197 51 182 4.55e-21 88.2
MS.gene33185.t1 MTR_2g023130 34.132 167 67 3 41 204 30 156 6.53e-21 89.0
MS.gene33185.t1 MTR_4g057300 38.281 128 75 2 81 204 2 129 1.24e-19 87.4
MS.gene33185.t1 MTR_3g030230 41.346 104 57 2 77 176 68 171 1.97e-19 83.6
MS.gene33185.t1 MTR_2g067930 35.294 153 89 3 57 199 44 196 3.60e-19 83.6
MS.gene33185.t1 MTR_8g107490 33.333 177 107 5 9 175 7 182 5.23e-19 85.1
MS.gene33185.t1 MTR_1g058140 37.615 109 67 1 92 199 89 197 9.80e-19 83.2
MS.gene33185.t1 MTR_3g067290 35.252 139 85 3 56 190 83 220 1.38e-18 82.4
MS.gene33185.t1 MTR_4g057975 32.099 162 98 5 21 176 43 198 1.95e-18 81.6
MS.gene33185.t1 MTR_7g065910 35.385 130 69 3 81 204 34 154 2.80e-18 84.0
MS.gene33185.t1 MTR_8g445570 35.849 106 66 2 100 204 6 110 5.04e-18 78.2
MS.gene33185.t1 MTR_0156s0040 38.462 130 74 2 81 204 2 131 5.47e-18 80.1
MS.gene33185.t1 MTR_7g050385 36.134 119 72 2 32 146 111 229 8.20e-18 80.9
MS.gene33185.t1 MTR_3g462670 32.237 152 98 3 57 204 38 188 1.05e-17 80.1
MS.gene33185.t1 MTR_5g079560 31.169 154 95 5 35 178 74 226 2.40e-17 79.7
MS.gene33185.t1 MTR_4g100470 38.835 103 63 0 102 204 71 173 3.77e-17 78.2
MS.gene33185.t1 MTR_1g098990 32.941 170 84 3 35 203 60 200 4.05e-17 79.7
MS.gene33185.t1 MTR_7g106560 37.113 97 60 1 109 204 92 188 5.01e-17 77.8
MS.gene33185.t1 MTR_0116s0050 30.899 178 89 4 20 196 27 171 6.75e-17 76.6
MS.gene33185.t1 MTR_8g103800 30.220 182 101 4 21 201 43 199 1.47e-16 78.2
MS.gene33185.t1 MTR_8g009330 35.915 142 71 5 25 157 76 206 2.37e-16 76.6
MS.gene33185.t1 MTR_1g043440 36.170 141 69 4 121 255 176 301 4.41e-16 77.0
MS.gene33185.t1 MTR_5g072970 38.298 94 58 0 111 204 33 126 9.18e-16 75.9
MS.gene33185.t1 MTR_7g034570 38.739 111 62 2 81 190 76 181 5.96e-15 72.0
MS.gene33185.t1 MTR_4g113170 37.079 89 55 1 110 197 74 162 7.20e-15 74.7
MS.gene33185.t1 MTR_5g060540 29.333 150 85 1 35 184 42 170 1.03e-14 73.9
MS.gene33185.t1 MTR_6g089230 34.211 114 56 3 92 204 38 133 1.27e-14 72.0
MS.gene33185.t1 MTR_7g010970 40.217 92 48 3 84 169 5 95 2.99e-14 70.9
MS.gene33185.t1 MTR_5g046245 39.175 97 58 1 109 204 6 102 8.32e-14 69.3
MS.gene33185.t1 MTR_7g024830 40.845 71 42 0 134 204 1 71 9.31e-14 65.5
MS.gene33185.t1 MTR_5g073000 36.000 100 64 0 109 208 109 208 9.50e-14 71.2
MS.gene33185.t1 MTR_7g056020 25.405 185 112 3 25 204 373 536 9.93e-14 71.2
MS.gene33185.t1 MTR_7g064870 38.938 113 61 3 46 152 54 164 2.38e-13 67.4
MS.gene33185.t1 MTR_2g027000 42.466 73 42 0 121 193 101 173 2.87e-13 67.0
MS.gene33185.t1 MTR_5g070810 38.000 100 55 3 60 153 14 112 3.07e-13 65.5
MS.gene33185.t1 MTR_8g020433 31.933 119 80 1 92 209 70 188 4.82e-13 68.9
MS.gene33185.t1 MTR_3g437000 37.500 88 52 1 32 116 38 125 9.49e-13 64.3
MS.gene33185.t1 MTR_6g081290 42.708 96 51 2 81 173 3 97 1.06e-12 63.2
MS.gene33185.t1 MTR_8g016360 31.618 136 85 3 16 146 1 133 1.47e-12 63.9
MS.gene33185.t1 MTR_4g051638 30.579 121 80 2 32 148 120 240 1.61e-12 66.2
MS.gene33185.t1 MTR_8g038805 39.744 78 43 2 83 156 55 132 4.25e-12 62.8
MS.gene33185.t1 MTR_7g050365 35.246 122 53 4 86 204 20 118 7.35e-12 63.5
MS.gene33185.t1 MTR_1g080040 35.789 95 55 2 81 169 1 95 8.62e-12 62.0
MS.gene33185.t1 MTR_1g052890 37.037 81 51 0 121 201 13 93 2.79e-11 63.2
MS.gene33185.t1 MTR_3g467720 33.010 103 56 1 88 190 49 138 5.71e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACCAACACTTCAGATTT+TGG 0.127175 1.2:-8900810 None:intergenic
GGACATGGCGCCATCGTTTC+TGG 0.166049 1.2:-8901075 None:intergenic
GCCAGAGTTGGAAGCGATTC+AGG 0.243223 1.2:+8900878 MS.gene33185:CDS
CAGAGAAGGAGAAGGAAAAT+TGG 0.276242 1.2:+8901103 MS.gene33185:CDS
CGAGGTGAAGCAGCCAAGTT+AGG 0.314453 1.2:+8900384 MS.gene33185:CDS
TCATTTAAACGGCCTGCAAT+AGG 0.361423 1.2:-8900636 None:intergenic
TTAGAAGAACAGCCTATTGC+AGG 0.373206 1.2:+8900624 MS.gene33185:CDS
TCTGGCAAAATAGATATATC+TGG 0.373242 1.2:-8900861 None:intergenic
TCGATTACGAAATTCTTTAT+CGG 0.375032 1.2:-8900340 None:intergenic
TGAGTGTCCATTCAGATTGC+GGG 0.385405 1.2:+8900527 MS.gene33185:CDS
ATATCTGGTTGATCTTCCTT+TGG 0.393013 1.2:-8900846 None:intergenic
TCATCTCTTCCTCATTTAAA+CGG 0.399399 1.2:-8900647 None:intergenic
TCGACTGGTTCAGCACTGTT+AGG 0.410500 1.2:-8900285 None:intergenic
ACCTGAATCGCTTCCAACTC+TGG 0.412695 1.2:-8900879 None:intergenic
TTTCTCCGTCAACCCTTGTC+AGG 0.435693 1.2:+8897922 MS.gene33185:CDS
AACAATACCAAACCCTAACT+TGG 0.442441 1.2:-8900397 None:intergenic
GAAGCAGCCAAGTTAGGGTT+TGG 0.454402 1.2:+8900390 MS.gene33185:CDS
GGTATGGAGGATCCCTCTGC+TGG 0.464129 1.2:+8900255 MS.gene33185:intron
TCCTTCTCTGCAGGAGGACA+TGG 0.465704 1.2:-8901090 None:intergenic
CTCTTATACTTGAATGCAAA+AGG 0.466270 1.2:+8900451 MS.gene33185:CDS
ATTTCAAAGTCAAATAATAG+AGG 0.466691 1.2:+8900420 MS.gene33185:CDS
GACATGGCGCCATCGTTTCT+GGG 0.467856 1.2:-8901074 None:intergenic
TCCTTCTCCTTCTCTGCAGG+AGG 0.477318 1.2:-8901096 None:intergenic
AGAGAAGGAGAAGGAAAATT+GGG 0.484008 1.2:+8901104 MS.gene33185:CDS
AAATAACCCCGCAATCTGAA+TGG 0.485974 1.2:-8900534 None:intergenic
GAGGTATAACAAGTCTATGA+AGG 0.500697 1.2:+8900785 MS.gene33185:CDS
CACCTCGAACCCACTTCAGC+AGG 0.515291 1.2:-8900368 None:intergenic
AAAACACAATCATGAGATGA+TGG 0.519225 1.2:+8900596 MS.gene33185:CDS
GAGGTGAAGCAGCCAAGTTA+GGG 0.528533 1.2:+8900385 MS.gene33185:CDS
AGATTTATAATGCACGTTCG+AGG 0.531682 1.2:+8900766 MS.gene33185:CDS
TATTGCAGGCCGTTTAAATG+AGG 0.536027 1.2:+8900638 MS.gene33185:CDS
GCCATGTCCTCCTGCAGAGA+AGG 0.538358 1.2:+8901089 MS.gene33185:CDS
ATTTACCTGACAAGGGTTGA+CGG 0.539125 1.2:-8897927 None:intergenic
TAGTGAATATTTACCTGACA+AGG 0.545419 1.2:-8897935 None:intergenic
ATCGAGATGACCTGCTGAAG+TGG 0.550843 1.2:+8900358 MS.gene33185:CDS
ATTACGAAATTCTTTATCGG+TGG 0.564422 1.2:-8900337 None:intergenic
TGGGAAGTTACCGTAGTATG+TGG 0.565876 1.2:+8900573 MS.gene33185:CDS
GACCTGCTGAAGTGGGTTCG+AGG 0.578355 1.2:+8900366 MS.gene33185:CDS
GTGAGTGTCCATTCAGATTG+CGG 0.585943 1.2:+8900526 MS.gene33185:CDS
AAATCAAGAGACTCATCGAC+TGG 0.594128 1.2:-8900300 None:intergenic
AGAAAAGAAGAGCTGACGAT+GGG 0.596743 1.2:+8900730 MS.gene33185:CDS
AAGAAAAGAAGAGCTGACGA+TGG 0.604104 1.2:+8900729 MS.gene33185:CDS
AGGTATAACAAGTCTATGAA+GGG 0.614739 1.2:+8900786 MS.gene33185:CDS
GGTAACTTCCCATACGCCAG+AGG 0.621236 1.2:-8900562 None:intergenic
TGATGATGACGGTGTGCCAA+AGG 0.622138 1.2:+8900830 MS.gene33185:CDS
TGATACCATTTCAGGTATGG+AGG 0.622154 1.2:+8900242 MS.gene33185:intron
TCCTCCTGCAGAGAAGGAGA+AGG 0.625108 1.2:+8901095 MS.gene33185:CDS
TCGAGATGACCTGCTGAAGT+GGG 0.628333 1.2:+8900359 MS.gene33185:CDS
TTAGGCTTCAAACCAGCAGA+GGG 0.633057 1.2:-8900267 None:intergenic
GGGTCCAAAATCTGAAGTGT+TGG 0.635922 1.2:+8900806 MS.gene33185:CDS
ATACTTGAATGCAAAAGGAG+TGG 0.641681 1.2:+8900456 MS.gene33185:CDS
GTAACTTCCCATACGCCAGA+GGG 0.653240 1.2:-8900561 None:intergenic
AATAGCTTTCCCAGAAACGA+TGG 0.655369 1.2:+8901065 MS.gene33185:CDS
AGTGAATATTTACCTGACAA+GGG 0.658458 1.2:-8897934 None:intergenic
GAGTGTCCATTCAGATTGCG+GGG 0.660517 1.2:+8900528 MS.gene33185:CDS
GTTAGGCTTCAAACCAGCAG+AGG 0.669794 1.2:-8900268 None:intergenic
GAAGTGTTGGTTGATGATGA+CGG 0.720616 1.2:+8900819 MS.gene33185:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTATAGAATTTTAAAAC+AGG + chr1.2:8899417-8899436 MS.gene33185:intron 10.0%
!!! TTTATTTTAATTTTCAGATT+CGG + chr1.2:8900989-8901008 MS.gene33185:intron 10.0%
!! AATTGTGCAAATATAATTAA+TGG - chr1.2:8900045-8900064 None:intergenic 15.0%
!! TTAATAGGCAAATTAAAATT+TGG - chr1.2:8899544-8899563 None:intergenic 15.0%
!!! AAAGTCATAAGAATATTTTT+TGG - chr1.2:8900708-8900727 None:intergenic 15.0%
!!! AAATATAATTAATGGATGAT+AGG - chr1.2:8900037-8900056 None:intergenic 15.0%
!!! AATTTTGAAAATATTTCAAG+AGG + chr1.2:8898204-8898223 MS.gene33185:intron 15.0%
!!! CAATCTTTTCATTTTAAAAA+GGG + chr1.2:8900160-8900179 MS.gene33185:intron 15.0%
!!! GTATTTTTATTCTAAGTATT+TGG + chr1.2:8899253-8899272 MS.gene33185:intron 15.0%
!!! TTATAAGACAATAACATTTT+GGG + chr1.2:8898941-8898960 MS.gene33185:intron 15.0%
!!! TTTATAAGACAATAACATTT+TGG + chr1.2:8898940-8898959 MS.gene33185:intron 15.0%
!!! TTTTTTATTATCAAGTTTGT+AGG + chr1.2:8898734-8898753 MS.gene33185:intron 15.0%
!! AAAAACTTAAATCCCTAAAA+CGG + chr1.2:8897990-8898009 MS.gene33185:intron 20.0%
!! AAACTGAAAAGTAATTACAT+TGG - chr1.2:8898150-8898169 None:intergenic 20.0%
!! AAAGTTAATTACAAGTCATA+CGG - chr1.2:8899753-8899772 None:intergenic 20.0%
!! ATAAACTTTGTAGTTATAAC+AGG + chr1.2:8899855-8899874 MS.gene33185:intron 20.0%
!! ATTAACTTTGTAGTTATAAC+AGG + chr1.2:8899764-8899783 MS.gene33185:intron 20.0%
!! ATTTCAAAGTCAAATAATAG+AGG + chr1.2:8900420-8900439 MS.gene33185:CDS 20.0%
!! TAACATAAACACTCAAAATT+TGG - chr1.2:8899798-8899817 None:intergenic 20.0%
!!! AACACACATTTTGTAAAATA+AGG - chr1.2:8900002-8900021 None:intergenic 20.0%
!!! ACACACATTTTGTAAAATAA+GGG - chr1.2:8900001-8900020 None:intergenic 20.0%
!!! CATTTTTACAAATGCAAAAT+GGG + chr1.2:8899350-8899369 MS.gene33185:intron 20.0%
!!! CCAATCTTTTCATTTTAAAA+AGG + chr1.2:8900159-8900178 MS.gene33185:intron 20.0%
!!! CCTTTTTAAAATGAAAAGAT+TGG - chr1.2:8900162-8900181 None:intergenic 20.0%
!!! CTTTGTAATTTTATAAATGC+AGG + chr1.2:8899378-8899397 MS.gene33185:intron 20.0%
!!! GTTTTTATTATCAAGTTTGT+TGG + chr1.2:8898621-8898640 MS.gene33185:intron 20.0%
!!! TATTTTTACTGTTTCATCAT+CGG + chr1.2:8899322-8899341 MS.gene33185:intron 20.0%
!!! TCATAAGAATATTTTTTGGA+CGG - chr1.2:8900704-8900723 None:intergenic 20.0%
!!! TCATTTTTACAAATGCAAAA+TGG + chr1.2:8899349-8899368 MS.gene33185:intron 20.0%
!!! TCTGGTAAATTATTTAGTTT+TGG + chr1.2:8898997-8899016 MS.gene33185:intron 20.0%
!!! TTTAGTGAATTTTCTCAATT+GGG + chr1.2:8899593-8899612 MS.gene33185:intron 20.0%
!!! TTTTCTAGATCTAAATGTTA+GGG + chr1.2:8898093-8898112 MS.gene33185:intron 20.0%
!!! TTTTTCTAGATCTAAATGTT+AGG + chr1.2:8898092-8898111 MS.gene33185:intron 20.0%
! AAATCTTAACACCAAATTTG+CGG - chr1.2:8898444-8898463 None:intergenic 25.0%
! AACAAACAAGGTGTAAATTT+AGG - chr1.2:8898026-8898045 None:intergenic 25.0%
! AATTAAACCCAAAAACACAT+TGG + chr1.2:8898847-8898866 MS.gene33185:intron 25.0%
! ATCATGTTCATGAGAAAAAT+AGG - chr1.2:8900226-8900245 None:intergenic 25.0%
! ATTCAATAGTAATTCTGTTC+TGG + chr1.2:8900905-8900924 MS.gene33185:intron 25.0%
! CTTGAAACAAATCAACAAAT+TGG + chr1.2:8899089-8899108 MS.gene33185:intron 25.0%
! TAAAAATACAGTGTTCAATG+TGG - chr1.2:8899242-8899261 None:intergenic 25.0%
! TATGATCCTTGTGTTATATT+TGG + chr1.2:8898880-8898899 MS.gene33185:intron 25.0%
! TCGATTACGAAATTCTTTAT+CGG - chr1.2:8900343-8900362 None:intergenic 25.0%
!! AAAACTCAAGGGTAAAATTT+TGG + chr1.2:8898472-8898491 MS.gene33185:intron 25.0%
!! AAACTCAAGGGTAAAATTTT+GGG + chr1.2:8898473-8898492 MS.gene33185:intron 25.0%
!! ACTATGCATTTTTATAAGTG+TGG + chr1.2:8899283-8899302 MS.gene33185:intron 25.0%
!! AGAATTTGTTTCATCTAGAA+GGG - chr1.2:8900952-8900971 None:intergenic 25.0%
!! GTTTAGTGAATTTTCTCAAT+TGG + chr1.2:8899592-8899611 MS.gene33185:intron 25.0%
!!! ATTTTTTGAATCTTGTGTCT+TGG - chr1.2:8900506-8900525 None:intergenic 25.0%
!!! GTTTTGTTTTTACGTATTAC+TGG + chr1.2:8900121-8900140 MS.gene33185:intron 25.0%
!!! TTGTTTTATTTCCGCAAATT+TGG + chr1.2:8898430-8898449 MS.gene33185:intron 25.0%
!!! TTTAAGTTTTTACAGAGACA+CGG - chr1.2:8897981-8898000 None:intergenic 25.0%
AAAACACAATCATGAGATGA+TGG + chr1.2:8900596-8900615 MS.gene33185:CDS 30.0%
ACGCAAAAGAAACAATTAGA+AGG - chr1.2:8900103-8900122 None:intergenic 30.0%
AGGTATAACAAGTCTATGAA+GGG + chr1.2:8900786-8900805 MS.gene33185:CDS 30.0%
AGTGAATATTTACCTGACAA+GGG - chr1.2:8897937-8897956 None:intergenic 30.0%
ATACGTAACTTCTTTCAGTA+TGG + chr1.2:8899636-8899655 MS.gene33185:intron 30.0%
ATTACGAAATTCTTTATCGG+TGG - chr1.2:8900340-8900359 None:intergenic 30.0%
ATTGAATGTAATTGTCGTTG+TGG + chr1.2:8898304-8898323 MS.gene33185:intron 30.0%
ATTTGCAATTGCAAAACTCA+AGG + chr1.2:8898460-8898479 MS.gene33185:intron 30.0%
CAAGGAAATTAAGAATGTTC+TGG + chr1.2:8898979-8898998 MS.gene33185:intron 30.0%
CAAGTTTGTAGGATGAATAT+TGG + chr1.2:8898745-8898764 MS.gene33185:intron 30.0%
CTCTTATACTTGAATGCAAA+AGG + chr1.2:8900451-8900470 MS.gene33185:CDS 30.0%
GATGAATATTGACAGAATGT+AGG + chr1.2:8898643-8898662 MS.gene33185:intron 30.0%
GTACAACCAAATATAACACA+AGG - chr1.2:8898889-8898908 None:intergenic 30.0%
TAAATGAGAGTTGAAGATGA+CGG - chr1.2:8899960-8899979 None:intergenic 30.0%
TAGTGAATATTTACCTGACA+AGG - chr1.2:8897938-8897957 None:intergenic 30.0%
TAGTGACAAAATAGCACTAT+CGG - chr1.2:8899458-8899477 None:intergenic 30.0%
TCAAATATCACCAGTAACTT+TGG + chr1.2:8899214-8899233 MS.gene33185:intron 30.0%
TCAAGTATGTCAATTGATGT+TGG + chr1.2:8899684-8899703 MS.gene33185:intron 30.0%
TCATCTCTTCCTCATTTAAA+CGG - chr1.2:8900650-8900669 None:intergenic 30.0%
TCTGGCAAAATAGATATATC+TGG - chr1.2:8900864-8900883 None:intergenic 30.0%
TTTGCAATTGCAAAACTCAA+GGG + chr1.2:8898461-8898480 MS.gene33185:intron 30.0%
! AAAAAACCTTACCTTTTGCT+AGG - chr1.2:8898541-8898560 None:intergenic 30.0%
! AAAAACCTTACCTTTTGCTA+GGG - chr1.2:8898540-8898559 None:intergenic 30.0%
! ATTCTGAACCAATGTGTTTT+TGG - chr1.2:8898858-8898877 None:intergenic 30.0%
! ATTGTGTTTTCCACATACTA+CGG - chr1.2:8900586-8900605 None:intergenic 30.0%
! GAGAATTTGTTTCATCTAGA+AGG - chr1.2:8900953-8900972 None:intergenic 30.0%
! TATATCTATTTTGCCAGAGT+TGG + chr1.2:8900866-8900885 MS.gene33185:CDS 30.0%
! TTAAGTTTTTACAGAGACAC+GGG - chr1.2:8897980-8897999 None:intergenic 30.0%
! TTCTGAACCAATGTGTTTTT+GGG - chr1.2:8898857-8898876 None:intergenic 30.0%
! TTTGAGTTTATGTCAAACAC+TGG + chr1.2:8899820-8899839 MS.gene33185:intron 30.0%
! TTTTCGTGAATGCTGTTAAT+AGG - chr1.2:8899559-8899578 None:intergenic 30.0%
!! ATTTTAAAAAGGGTCCAGAT+GGG + chr1.2:8900170-8900189 MS.gene33185:intron 30.0%
AAATGAGAGTTGAAGATGAC+GGG - chr1.2:8899959-8899978 None:intergenic 35.0%
AACAATACCAAACCCTAACT+TGG - chr1.2:8900400-8900419 None:intergenic 35.0%
AACTCCTATGCCAAAATAAG+AGG - chr1.2:8899035-8899054 None:intergenic 35.0%
AGAGAAGGAGAAGGAAAATT+GGG + chr1.2:8901104-8901123 MS.gene33185:CDS 35.0%
AGATTTATAATGCACGTTCG+AGG + chr1.2:8900766-8900785 MS.gene33185:CDS 35.0%
AGTAAGTACACATTCTGAGT+TGG - chr1.2:8900196-8900215 None:intergenic 35.0%
AGTGACAAAATAGCACTATC+GGG - chr1.2:8899457-8899476 None:intergenic 35.0%
ATACTTGAATGCAAAAGGAG+TGG + chr1.2:8900456-8900475 MS.gene33185:CDS 35.0%
ATATCTGGTTGATCTTCCTT+TGG - chr1.2:8900849-8900868 None:intergenic 35.0%
CATGAACATGATACCATTTC+AGG + chr1.2:8900234-8900253 MS.gene33185:intron 35.0%
CTGAAAGAAGTTACGTATCA+AGG - chr1.2:8899634-8899653 None:intergenic 35.0%
GAGGTATAACAAGTCTATGA+AGG + chr1.2:8900785-8900804 MS.gene33185:CDS 35.0%
TATTAGTAAACCTTCCGGTT+GGG + chr1.2:8899709-8899728 MS.gene33185:intron 35.0%
TATTGACAGAATGTAGGTTC+TGG + chr1.2:8898649-8898668 MS.gene33185:intron 35.0%
TGGTCTATTAGTAAACCTTC+CGG + chr1.2:8899704-8899723 MS.gene33185:intron 35.0%
! ACATGATACCATTTCAGGTA+TGG + chr1.2:8900239-8900258 MS.gene33185:intron 35.0%
! TATTGGAAGAGTGTAGATTG+TGG + chr1.2:8898762-8898781 MS.gene33185:intron 35.0%
! TCAACCAACACTTCAGATTT+TGG - chr1.2:8900813-8900832 None:intergenic 35.0%
!! CATTTTAAAAAGGGTCCAGA+TGG + chr1.2:8900169-8900188 MS.gene33185:intron 35.0%
!! TAACTGCAATCAGCTTGTTA+AGG + chr1.2:8899113-8899132 MS.gene33185:intron 35.0%
!! TCTGATCGATCCTCTTATTT+TGG + chr1.2:8899022-8899041 MS.gene33185:intron 35.0%
!!! ACATTTTGGGTTCGTGAATT+TGG + chr1.2:8898954-8898973 MS.gene33185:intron 35.0%
!!! CCTTGTTTGTTTTTTCTTGC+CGG + chr1.2:8898035-8898054 MS.gene33185:intron 35.0%
AAACGACACATATGCAGGAA+GGG - chr1.2:8898507-8898526 None:intergenic 40.0%
AAATAACCCCGCAATCTGAA+TGG - chr1.2:8900537-8900556 None:intergenic 40.0%
AAATCAAGAGACTCATCGAC+TGG - chr1.2:8900303-8900322 None:intergenic 40.0%
AAGAAAAGAAGAGCTGACGA+TGG + chr1.2:8900729-8900748 MS.gene33185:CDS 40.0%
AATAGCTTTCCCAGAAACGA+TGG + chr1.2:8901065-8901084 MS.gene33185:CDS 40.0%
AGAAAAGAAGAGCTGACGAT+GGG + chr1.2:8900730-8900749 MS.gene33185:CDS 40.0%
ATATTTGATCAGCGCGTTCA+TGG - chr1.2:8899202-8899221 None:intergenic 40.0%
ATTTACCTGACAAGGGTTGA+CGG - chr1.2:8897930-8897949 None:intergenic 40.0%
CAAAGAAACGACACATATGC+AGG - chr1.2:8898512-8898531 None:intergenic 40.0%
CAGAGAAGGAGAAGGAAAAT+TGG + chr1.2:8901103-8901122 MS.gene33185:CDS 40.0%
CCGGCAAGAAAAAACAAACA+AGG - chr1.2:8898038-8898057 None:intergenic 40.0%
CTATTAGTAAACCTTCCGGT+TGG + chr1.2:8899708-8899727 MS.gene33185:intron 40.0%
CTTTGTTCTACCCTAGCAAA+AGG + chr1.2:8898527-8898546 MS.gene33185:intron 40.0%
GGTATTTCACACATCCGAAT+TGG + chr1.2:8900926-8900945 MS.gene33185:intron 40.0%
TAACATGTCAGTGTCGTGTT+CGG - chr1.2:8898253-8898272 None:intergenic 40.0%
TATTGCAGGCCGTTTAAATG+AGG + chr1.2:8900638-8900657 MS.gene33185:CDS 40.0%
TCATTTAAACGGCCTGCAAT+AGG - chr1.2:8900639-8900658 None:intergenic 40.0%
TGTAATTGTCGTTGTGGTGT+TGG + chr1.2:8898310-8898329 MS.gene33185:intron 40.0%
TTAGAAGAACAGCCTATTGC+AGG + chr1.2:8900624-8900643 MS.gene33185:CDS 40.0%
TTCATCTAGAAGGGCCAATT+CGG - chr1.2:8900943-8900962 None:intergenic 40.0%
TTCTACCCTAGCAAAAGGTA+AGG + chr1.2:8898532-8898551 MS.gene33185:intron 40.0%
! AGGTGAAACGAAACCGTTTT+AGG - chr1.2:8898006-8898025 None:intergenic 40.0%
! GGTGAAACGAAACCGTTTTA+GGG - chr1.2:8898005-8898024 None:intergenic 40.0%
! TGATACCATTTCAGGTATGG+AGG + chr1.2:8900242-8900261 MS.gene33185:intron 40.0%
!! GAAGTGTTGGTTGATGATGA+CGG + chr1.2:8900819-8900838 MS.gene33185:CDS 40.0%
!!! CGATCCTCTTATTTTGGCAT+AGG + chr1.2:8899028-8899047 MS.gene33185:intron 40.0%
!!! TGTTTGTTTTTTCTTGCCGG+TGG + chr1.2:8898038-8898057 MS.gene33185:intron 40.0%
CAATGTGGCTCCAAAGTTAC+TGG - chr1.2:8899227-8899246 None:intergenic 45.0%
GAAACGACACATATGCAGGA+AGG - chr1.2:8898508-8898527 None:intergenic 45.0%
GTGAGTGTCCATTCAGATTG+CGG + chr1.2:8900526-8900545 MS.gene33185:CDS 45.0%
TGAGTGTCCATTCAGATTGC+GGG + chr1.2:8900527-8900546 MS.gene33185:CDS 45.0%
TGGGAAGTTACCGTAGTATG+TGG + chr1.2:8900573-8900592 MS.gene33185:CDS 45.0%
TTAGGCTTCAAACCAGCAGA+GGG - chr1.2:8900270-8900289 None:intergenic 45.0%
TTGCATTGCCTGAACAGCTA+GGG + chr1.2:8899153-8899172 MS.gene33185:intron 45.0%
! GGGTCCAAAATCTGAAGTGT+TGG + chr1.2:8900806-8900825 MS.gene33185:CDS 45.0%
! GGGTTCGTGAATTTGGTTCA+AGG + chr1.2:8898961-8898980 MS.gene33185:intron 45.0%
! TGTCATTTCCCTAGCTGTTC+AGG - chr1.2:8899164-8899183 None:intergenic 45.0%
! TTCTGATGGAAGGGCATGTT+CGG - chr1.2:8898339-8898358 None:intergenic 45.0%
! TTTTCCTTCTCCTTCTCTGC+AGG - chr1.2:8901102-8901121 None:intergenic 45.0%
!!! ATTTTTTGCCCTCTGGCGTA+TGG + chr1.2:8900553-8900572 MS.gene33185:CDS 45.0%
!!! TACGGCATCGACTTTTCAAC+AGG - chr1.2:8899735-8899754 None:intergenic 45.0%
!!! TTTTTTGCCCTCTGGCGTAT+GGG + chr1.2:8900554-8900573 MS.gene33185:CDS 45.0%
ACCCTAACAGCTTCTTCCAC+CGG - chr1.2:8898057-8898076 None:intergenic 50.0%
ACCTGAATCGCTTCCAACTC+TGG - chr1.2:8900882-8900901 None:intergenic 50.0%
ATCGAGATGACCTGCTGAAG+TGG + chr1.2:8900358-8900377 MS.gene33185:CDS 50.0%
CACGGACACTTCTGATGGAA+GGG - chr1.2:8898348-8898367 None:intergenic 50.0%
CTTGCATTGCCTGAACAGCT+AGG + chr1.2:8899152-8899171 MS.gene33185:intron 50.0%
GAAGCAGCCAAGTTAGGGTT+TGG + chr1.2:8900390-8900409 MS.gene33185:CDS 50.0%
GAGGTGAAGCAGCCAAGTTA+GGG + chr1.2:8900385-8900404 MS.gene33185:CDS 50.0%
GAGTGTCCATTCAGATTGCG+GGG + chr1.2:8900528-8900547 MS.gene33185:CDS 50.0%
GGGATCCTCCATACCTGAAA+TGG - chr1.2:8900250-8900269 None:intergenic 50.0%
GTAACTTCCCATACGCCAGA+GGG - chr1.2:8900564-8900583 None:intergenic 50.0%
GTTAGGCTTCAAACCAGCAG+AGG - chr1.2:8900271-8900290 None:intergenic 50.0%
TACAGAGACACGGGAGAGAA+GGG - chr1.2:8897971-8897990 None:intergenic 50.0%
TCGAGATGACCTGCTGAAGT+GGG + chr1.2:8900359-8900378 MS.gene33185:CDS 50.0%
TTACAGAGACACGGGAGAGA+AGG - chr1.2:8897972-8897991 None:intergenic 50.0%
TTACTCCAACCTCCGTAGCA+CGG - chr1.2:8898366-8898385 None:intergenic 50.0%
TTTCTCCGTCAACCCTTGTC+AGG + chr1.2:8897922-8897941 MS.gene33185:CDS 50.0%
! ACATTCTGAGTTGGCCCATC+TGG - chr1.2:8900187-8900206 None:intergenic 50.0%
! GACTTTTCAACAGGCCCAAC+CGG - chr1.2:8899726-8899745 None:intergenic 50.0%
! TCGACTGGTTCAGCACTGTT+AGG - chr1.2:8900288-8900307 None:intergenic 50.0%
!! CATCAGAAGTGTCCGTGCTA+CGG + chr1.2:8898351-8898370 MS.gene33185:intron 50.0%
!! TGATGATGACGGTGTGCCAA+AGG + chr1.2:8900830-8900849 MS.gene33185:CDS 50.0%
!!! CGGGGTTATTTTTTGCCCTC+TGG + chr1.2:8900546-8900565 MS.gene33185:CDS 50.0%
CGAGGTGAAGCAGCCAAGTT+AGG + chr1.2:8900384-8900403 MS.gene33185:CDS 55.0%
CGTAGCACGGACACTTCTGA+TGG - chr1.2:8898353-8898372 None:intergenic 55.0%
GACATGGCGCCATCGTTTCT+GGG - chr1.2:8901077-8901096 None:intergenic 55.0%
GCACGGACACTTCTGATGGA+AGG - chr1.2:8898349-8898368 None:intergenic 55.0%
GCCAGAGTTGGAAGCGATTC+AGG + chr1.2:8900878-8900897 MS.gene33185:CDS 55.0%
GCCGGTGGAAGAAGCTGTTA+GGG + chr1.2:8898053-8898072 MS.gene33185:intron 55.0%
GGTAACTTCCCATACGCCAG+AGG - chr1.2:8900565-8900584 None:intergenic 55.0%
TCCTCCTGCAGAGAAGGAGA+AGG + chr1.2:8901095-8901114 MS.gene33185:CDS 55.0%
TCCTTCTCCTTCTCTGCAGG+AGG - chr1.2:8901099-8901118 None:intergenic 55.0%
TCCTTCTCTGCAGGAGGACA+TGG - chr1.2:8901093-8901112 None:intergenic 55.0%
TGCCGGTGGAAGAAGCTGTT+AGG + chr1.2:8898052-8898071 MS.gene33185:intron 55.0%
TTTCAACAGGCCCAACCGGA+AGG - chr1.2:8899722-8899741 None:intergenic 55.0%
AGTGTCCGTGCTACGGAGGT+TGG + chr1.2:8898358-8898377 MS.gene33185:intron 60.0%
CACCTCGAACCCACTTCAGC+AGG - chr1.2:8900371-8900390 None:intergenic 60.0%
GCCATGTCCTCCTGCAGAGA+AGG + chr1.2:8901089-8901108 MS.gene33185:CDS 60.0%
GGACATGGCGCCATCGTTTC+TGG - chr1.2:8901078-8901097 None:intergenic 60.0%
GGTATGGAGGATCCCTCTGC+TGG + chr1.2:8900255-8900274 MS.gene33185:intron 60.0%
! GACCTGCTGAAGTGGGTTCG+AGG + chr1.2:8900366-8900385 MS.gene33185:CDS 60.0%
!! CAGAAGTGTCCGTGCTACGG+AGG + chr1.2:8898354-8898373 MS.gene33185:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 8897901 8901158 8897901 ID=MS.gene33185
chr1.2 mRNA 8897901 8901158 8897901 ID=MS.gene33185.t1;Parent=MS.gene33185
chr1.2 exon 8897901 8897943 8897901 ID=MS.gene33185.t1.exon1;Parent=MS.gene33185.t1
chr1.2 CDS 8897901 8897943 8897901 ID=cds.MS.gene33185.t1;Parent=MS.gene33185.t1
chr1.2 exon 8900256 8900899 8900256 ID=MS.gene33185.t1.exon2;Parent=MS.gene33185.t1
chr1.2 CDS 8900256 8900899 8900256 ID=cds.MS.gene33185.t1;Parent=MS.gene33185.t1
chr1.2 exon 8901006 8901158 8901006 ID=MS.gene33185.t1.exon3;Parent=MS.gene33185.t1
chr1.2 CDS 8901006 8901158 8901006 ID=cds.MS.gene33185.t1;Parent=MS.gene33185.t1
Gene Sequence

>MS.gene33185

ATGACGGATCGACACAAACATTTTCTCCGTCAACCCTTGTCAGGTAAATATTCACTACTCGTTCATTCCCTTCTCTCCCGTGTCTCTGTAAAAACTTAAATCCCTAAAACGGTTTCGTTTCACCTAAATTTACACCTTGTTTGTTTTTTCTTGCCGGTGGAAGAAGCTGTTAGGGTTTTTATCTTACTCGATTTTTCTAGATCTAAATGTTAGGGTTTTCAGTACATGCATTATCTACTTTCATATCCAATGTAATTACTTTTCAGTTTGTTTTATTTTCATATATCATGATCTTAGTACTTCAATTTTGAAAATATTTCAAGAGGAGTATGAAGCACATACACTGACACCGAACACGACACTGACATGTTAGTATCGTTCAATTTTTTTTAAAAATTAATTAATTGAATGTAATTGTCGTTGTGGTGTTGGACGCCGAACATGCCCTTCCATCAGAAGTGTCCGTGCTACGGAGGTTGGAGTAATTCTCATATATTTTCTTTTTGAAATTCAAGTCGTGCTTTTTATTTTGTTTTATTTCCGCAAATTTGGTGTTAAGATTTGCAATTGCAAAACTCAAGGGTAAAATTTTGGGTTTCATTTCCCTTCCTGCATATGTGTCGTTTCTTTGTTCTACCCTAGCAAAAGGTAAGGTTTTTTTTTTTTTTTTTTTTGTTTTGTTGTATCTTATTATTCAAATCATTCTTTGTTTCAGACTCTGTTTTTATTATCAAGTTTGTTGGATGAATATTGACAGAATGTAGGTTCTGGCTGATATTTAGCTTTTGTTGTATCTTATTATTCATATTTTTCTTTGTTTTAGACTTCATGTGTTTTTTATTATCAAGTTTGTAGGATGAATATTGGAAGAGTGTAGATTGTGGCTGAGATTTAGCTCTGTTCTTGTATTTGTTCTTTAAACATGTGTAAAATAATTCTTCTTGTTAATTAAACCCAAAAACACATTGGTTCAGAATGTTATGATCCTTGTGTTATATTTGGTTGTACATTGTTCTTTTGTTCTAGTACAAGTCTTTAATTTATAAGACAATAACATTTTGGGTTCGTGAATTTGGTTCAAGGAAATTAAGAATGTTCTGGTAAATTATTTAGTTTTGGAGTCTGATCGATCCTCTTATTTTGGCATAGGAGTTAAGTGTGTAATTTTTTTATGCATAGAGATGAAGTCTTGAAACAAATCAACAAATTGGTTAACTGCAATCAGCTTGTTAAGGTGATATTATTAATTTGCTTGCATTGCCTGAACAGCTAGGGAAATGACATCAAGTGTCAATATTCCATGAACGCGCTGATCAAATATCACCAGTAACTTTGGAGCCACATTGAACACTGTATTTTTATTCTAAGTATTTGGAGAGAGAACTATGCATTTTTATAAGTGTGGAATCATATTTGCTTTGTATTTTTACTGTTTCATCATCGGAACTTCATTTTTACAAATGCAAAATGGGATGTGCTTTGTAATTTTATAAATGCAGGATATTAGAAAAGCAATATTTTATAGAATTTTAAAACAGGTTTCTAGTACTCAACCCGATAGTGCTATTTTGTCACTATTACTGTATAATTTTTATTTTATGTAAATTATTTTTCTTACATGTTTTTAGCTGTTGCATTTGCCAAATTTTAATTTGCCTATTAACAGCATTCACGAAAAAAGTTGATTTTCTGTTTAGTGAATTTTCTCAATTGGGTAAATGAATATGCAGCCTTGATACGTAACTTCTTTCAGTATGGATTTCATTATATGCAGAGAATGTTATCAAGTATGTCAATTGATGTTGGTCTATTAGTAAACCTTCCGGTTGGGCCTGTTGAAAAGTCGATGCCGTATGACTTGTAATTAACTTTGTAGTTATAACAGGAATATTAGCCAAATTTTGAGTGTTTATGTTATTTTTGAGTTTATGTCAAACACTGGAAAATACATGATATAAACTTTGTAGTTATAACAGGAATATTAGCCAAATTTTGAGTGTTTATGTTATTTTTGAGTTTATGTCAAACACTGGAAAATACATGATATATTTGAAACCCGTCATCTTCAACTCTCATTTAAAATCACTCTAAATAAAACCCTTATTTTACAAAATGTGTGTTTCATTACTCATTCCTATCATCCATTAATTATATTTGCACAATTTTCATTTGAAAGCAAATGAAACGTGACTCTAATTTCCTTCTAATTGTTTCTTTTGCGTTTTGTTTTTACGTATTACTGGTATCGTTTGAATAAGCCAATCTTTTCATTTTAAAAAGGGTCCAGATGGGCCAACTCAGAATGTGTACTTACTGTATAAGCCTATTTTTCTCATGAACATGATACCATTTCAGGTATGGAGGATCCCTCTGCTGGTTTGAAGCCTAACAGTGCTGAACCAGTCGATGAGTCTCTTGATTTCGCTAAACACTTTGCCACCGATAAAGAATTTCGTAATCGAGATGACCTGCTGAAGTGGGTTCGAGGTGAAGCAGCCAAGTTAGGGTTTGGTATTGTTATTTCAAAGTCAAATAATAGAGGAACACAAACTCTTATACTTGAATGCAAAAGGAGTGGTGTATATAAGAAGCAGTCGAAACGCCAAGACACAAGATTCAAAAAATGTGAGTGTCCATTCAGATTGCGGGGTTATTTTTTGCCCTCTGGCGTATGGGAAGTTACCGTAGTATGTGGAAAACACAATCATGAGATGATGGAAAATTTAGAAGAACAGCCTATTGCAGGCCGTTTAAATGAGGAAGAGATGAAACTCGTTCATGAGATGACAAATAACACTGTCCGTCCAAAAAATATTCTTATGACTTTGAAGAAAAGAAGAGCTGACGATGGGATTACTATCAAGCAGATTTATAATGCACGTTCGAGGTATAACAAGTCTATGAAGGGTCCAAAATCTGAAGTGTTGGTTGATGATGACGGTGTGCCAAAGGAAGATCAACCAGATATATCTATTTTGCCAGAGTTGGAAGCGATTCAGGTTTGATTCAATAGTAATTCTGTTCTGGTATTTCACACATCCGAATTGGCCCTTCTAGATGAAACAAATTCTCATTAAATTTTTTATTTTTTATTTTAATTTTCAGATTCGGTTTAACAATGCTGATTACAATACGAAGATACTTATTAAAGAGCAGCTGCATCAAATAGCTTTCCCAGAAACGATGGCGCCATGTCCTCCTGCAGAGAAGGAGAAGGAAAATTGGGACTTTTATGATTTAGAAACTTGTGAAATATGA
Protein sequence

>MS.gene33185.t1

MTDRHKHFLRQPLSGMEDPSAGLKPNSAEPVDESLDFAKHFATDKEFRNRDDLLKWVRGEAAKLGFGIVISKSNNRGTQTLILECKRSGVYKKQSKRQDTRFKKCECPFRLRGYFLPSGVWEVTVVCGKHNHEMMENLEEQPIAGRLNEEEMKLVHEMTNNTVRPKNILMTLKKRRADDGITIKQIYNARSRYNKSMKGPKSEVLVDDDGVPKEDQPDISILPELEAIQIRFNNADYNTKILIKEQLHQIAFPETMAPCPPAEKEKENWDFYDLETCEI