AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33478

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33478.t1 MTR_4g015080 98.097 473 9 0 1 473 1 473 0.0 967
MS.gene33478.t1 MTR_7g067010 85.033 461 69 0 13 473 3 463 0.0 833
MS.gene33478.t1 MTR_3g462710 70.905 464 133 2 12 473 2 465 0.0 682
MS.gene33478.t1 MTR_3g462710 71.067 356 101 2 120 473 1 356 0.0 515
MS.gene33478.t1 MTR_3g112470 48.491 464 226 5 13 473 2 455 2.63e-149 438
MS.gene33478.t1 MTR_3g112460 48.276 464 227 5 13 473 2 455 4.14e-149 437
MS.gene33478.t1 MTR_7g098200 44.894 470 244 5 12 474 9 470 1.73e-143 424
MS.gene33478.t1 MTR_7g098180 43.983 482 253 7 1 474 1 473 9.19e-139 412
MS.gene33478.t1 MTR_7g098200 44.681 470 235 7 12 474 9 460 1.42e-138 411
MS.gene33478.t1 MTR_3g112450 47.588 456 223 6 23 473 12 456 1.17e-137 408
MS.gene33478.t1 MTR_7g098220 44.989 469 245 3 13 474 9 471 2.52e-137 408
MS.gene33478.t1 MTR_7g098040 43.154 482 258 5 1 474 2 475 1.52e-135 404
MS.gene33478.t1 MTR_3g072300 44.421 466 251 5 12 474 4 464 1.69e-135 403
MS.gene33478.t1 MTR_7g098160 42.116 482 263 5 1 474 1 474 2.02e-135 404
MS.gene33478.t1 MTR_5g012290 46.405 459 236 7 24 473 17 474 4.09e-134 400
MS.gene33478.t1 MTR_7g098090 41.684 487 263 6 1 474 1 479 7.52e-132 395
MS.gene33478.t1 MTR_1g075440 44.492 472 244 7 10 473 7 468 2.17e-131 393
MS.gene33478.t1 MTR_7g098150 42.094 468 255 5 1 460 1 460 3.43e-131 389
MS.gene33478.t1 MTR_5g055000 44.889 450 238 6 32 474 16 462 3.69e-131 392
MS.gene33478.t1 MTR_7g088825 42.161 472 263 5 10 474 1 469 2.29e-130 391
MS.gene33478.t1 MTR_4g101380 45.022 462 241 6 24 473 17 477 1.92e-129 388
MS.gene33478.t1 MTR_7g010820 43.933 478 247 6 10 473 2 472 2.52e-128 385
MS.gene33478.t1 MTR_7g098230 44.875 439 229 4 43 474 35 467 1.42e-127 383
MS.gene33478.t1 MTR_1g041875 42.887 471 249 6 13 474 4 463 5.73e-127 381
MS.gene33478.t1 MTR_5g012270 43.043 460 250 6 24 473 19 476 2.18e-125 377
MS.gene33478.t1 MTR_1g041835 44.276 463 246 5 17 473 10 466 6.42e-125 376
MS.gene33478.t1 MTR_1g041815 44.492 463 245 5 17 473 10 466 1.47e-123 373
MS.gene33478.t1 MTR_3g005010 43.103 464 230 7 16 474 12 446 2.60e-123 371
MS.gene33478.t1 MTR_7g098220 44.780 431 225 3 51 474 3 427 1.11e-121 367
MS.gene33478.t1 MTR_7g088790 41.685 463 261 5 20 474 15 476 1.95e-121 368
MS.gene33478.t1 MTR_4g019580 40.678 472 273 5 4 473 6 472 1.13e-120 365
MS.gene33478.t1 MTR_5g014150 41.505 465 261 5 18 473 28 490 3.07e-120 365
MS.gene33478.t1 MTR_4g033375 40.870 460 246 6 20 474 18 456 3.75e-120 361
MS.gene33478.t1 MTR_1g042310 40.938 469 266 5 12 474 4 467 9.11e-120 363
MS.gene33478.t1 MTR_7g006540 42.316 475 252 10 17 474 44 513 1.21e-118 362
MS.gene33478.t1 MTR_4g098800 41.127 479 268 6 3 473 5 477 1.71e-118 360
MS.gene33478.t1 MTR_4g098800 41.387 476 265 6 6 473 73 542 2.85e-118 361
MS.gene33478.t1 MTR_7g088820 39.278 471 276 6 11 474 5 472 8.99e-118 358
MS.gene33478.t1 MTR_3g069500 41.614 471 260 7 7 474 6 464 3.09e-117 356
MS.gene33478.t1 MTR_1g116930 40.268 447 258 4 30 473 19 459 3.38e-117 356
MS.gene33478.t1 MTR_4g033395 40.870 460 246 7 20 474 18 456 1.02e-116 352
MS.gene33478.t1 MTR_7g065080 39.496 476 270 7 1 473 1 461 1.88e-115 352
MS.gene33478.t1 MTR_5g038060 42.151 465 250 8 25 473 18 479 4.48e-115 352
MS.gene33478.t1 MTR_3g014790 41.970 467 241 6 19 474 4 451 9.59e-114 347
MS.gene33478.t1 MTR_4g033315 40.987 466 250 6 13 474 10 454 2.44e-113 345
MS.gene33478.t1 MTR_4g033310 40.987 466 250 6 13 474 10 454 2.44e-113 345
MS.gene33478.t1 MTR_4g033325 41.328 467 245 7 13 474 10 452 3.43e-113 345
MS.gene33478.t1 MTR_0294s0020 41.202 466 247 7 13 474 10 452 4.54e-113 345
MS.gene33478.t1 MTR_7g005930 39.485 466 269 8 14 473 13 471 1.38e-112 344
MS.gene33478.t1 MTR_8g038880 42.511 454 229 5 34 474 20 454 2.00e-112 343
MS.gene33478.t1 MTR_1g041885 42.623 427 229 5 53 474 1 416 2.30e-111 340
MS.gene33478.t1 MTR_3g069420 40.343 466 269 7 11 473 13 472 3.34e-111 341
MS.gene33478.t1 MTR_4g033330 41.004 478 254 7 1 474 1 454 5.61e-111 338
MS.gene33478.t1 MTR_7g013270 40.492 447 253 8 33 473 89 528 3.09e-108 335
MS.gene33478.t1 MTR_4g114340 43.949 471 244 9 12 473 2 461 4.70e-108 332
MS.gene33478.t1 MTR_3g069440 40.481 457 265 5 20 473 19 471 1.15e-106 329
MS.gene33478.t1 MTR_1g042310 40.698 430 244 5 51 474 6 430 6.25e-106 326
MS.gene33478.t1 MTR_7g088820 40.640 406 234 4 76 474 5 410 8.47e-106 325
MS.gene33478.t1 MTR_8g469480 39.644 449 256 6 38 474 42 487 7.54e-105 325
MS.gene33478.t1 MTR_1g042310 40.421 428 244 5 53 474 1 423 3.32e-104 322
MS.gene33478.t1 MTR_7g111260 38.063 444 260 6 32 474 17 446 5.55e-102 317
MS.gene33478.t1 MTR_8g087780 38.806 469 273 6 20 474 7 475 7.54e-102 317
MS.gene33478.t1 MTR_5g085850 41.719 477 252 10 6 473 3 462 3.45e-101 315
MS.gene33478.t1 MTR_2g017750 37.678 491 279 10 3 474 8 490 3.86e-100 313
MS.gene33478.t1 MTR_4g107580 37.100 469 278 8 18 473 11 475 4.21e-99 310
MS.gene33478.t1 MTR_7g098200 42.818 362 194 4 120 474 1 356 2.60e-98 305
MS.gene33478.t1 MTR_4g107510 37.086 453 262 7 38 473 40 486 1.48e-97 306
MS.gene33478.t1 MTR_3g100980 37.802 455 266 6 28 473 10 456 1.67e-97 305
MS.gene33478.t1 MTR_7g065080 40.885 384 214 5 92 473 2 374 1.01e-96 301
MS.gene33478.t1 MTR_4g107670 36.853 483 272 9 12 473 2 472 1.87e-96 302
MS.gene33478.t1 MTR_8g087810 38.511 470 274 6 20 474 7 476 2.42e-96 302
MS.gene33478.t1 MTR_1g085090 37.528 445 261 8 32 474 17 446 3.34e-96 303
MS.gene33478.t1 MTR_8g469310 39.177 462 270 7 21 473 13 472 3.63e-95 299
MS.gene33478.t1 MTR_2g005130 35.924 476 279 8 3 474 11 464 1.70e-94 298
MS.gene33478.t1 MTR_8g469470 35.135 481 298 5 13 480 11 490 1.72e-94 298
MS.gene33478.t1 MTR_4g107530 36.042 480 282 10 11 474 14 484 7.25e-94 296
MS.gene33478.t1 MTR_3g100980 35.611 483 266 7 28 473 10 484 1.92e-91 290
MS.gene33478.t1 MTR_7g088820 40.055 362 210 4 120 474 1 362 5.11e-90 283
MS.gene33478.t1 MTR_7g064780 34.810 474 297 6 11 475 3 473 8.94e-90 285
MS.gene33478.t1 MTR_5g038190 36.226 461 277 7 25 473 15 470 1.63e-89 285
MS.gene33478.t1 MTR_5g038380 36.207 464 284 7 20 473 14 475 2.25e-88 281
MS.gene33478.t1 MTR_4g107610 36.166 459 278 8 25 474 22 474 8.77e-87 278
MS.gene33478.t1 MTR_4g123537 36.345 476 282 9 2 474 13 470 1.03e-86 278
MS.gene33478.t1 MTR_1g009200 35.524 487 282 11 1 473 1 469 1.04e-85 275
MS.gene33478.t1 MTR_7g064800 36.266 466 269 12 28 475 20 475 2.54e-85 274
MS.gene33478.t1 MTR_4g033325 40.443 361 186 7 119 474 18 354 3.19e-84 267
MS.gene33478.t1 MTR_4g033385 39.889 361 191 7 119 474 16 355 1.07e-81 261
MS.gene33478.t1 MTR_4g033325 40.476 336 178 5 144 474 10 328 1.28e-80 257
MS.gene33478.t1 MTR_1g026740 33.987 459 285 10 26 473 7 458 2.42e-79 258
MS.gene33478.t1 MTR_8g103233 35.746 456 269 11 32 474 15 459 1.97e-77 253
MS.gene33478.t1 MTR_2g101650 32.914 477 297 11 12 474 2 469 8.86e-76 249
MS.gene33478.t1 MTR_1g026750 33.951 486 281 14 1 473 1 459 1.34e-74 245
MS.gene33478.t1 MTR_6g008690 36.344 454 243 9 40 474 13 439 6.20e-74 243
MS.gene33478.t1 MTR_2g101640 32.563 476 302 11 10 474 1 468 6.44e-74 245
MS.gene33478.t1 MTR_2g101610 34.868 456 276 11 32 474 25 472 1.11e-73 244
MS.gene33478.t1 MTR_2g101590 34.430 456 278 11 32 474 24 471 1.12e-73 244
MS.gene33478.t1 MTR_1g057460 34.444 450 267 12 32 474 42 470 1.28e-70 235
MS.gene33478.t1 MTR_1g026770 32.511 446 286 9 36 473 17 455 1.62e-69 232
MS.gene33478.t1 MTR_4g024930 34.874 476 270 13 11 474 11 458 4.33e-68 228
MS.gene33478.t1 MTR_4g011280 32.222 450 286 10 36 474 34 475 1.71e-66 224
MS.gene33478.t1 MTR_1g057460 35.000 420 245 12 62 474 1 399 2.25e-66 222
MS.gene33478.t1 MTR_7g098210 34.419 430 189 11 53 474 1 345 8.97e-63 211
MS.gene33478.t1 MTR_2g101560 31.291 457 291 9 32 474 25 472 2.96e-62 214
MS.gene33478.t1 MTR_0236s0050 34.368 419 249 11 62 474 1 399 6.60e-62 210
MS.gene33478.t1 MTR_2g093160 30.556 468 299 8 20 474 2 456 1.05e-60 208
MS.gene33478.t1 MTR_2g093180 30.638 470 297 9 20 474 2 457 5.83e-58 201
MS.gene33478.t1 MTR_2g093640 28.450 471 302 9 19 474 6 456 3.01e-53 188
MS.gene33478.t1 MTR_7g051880 28.947 456 290 8 19 474 10 431 3.82e-52 184
MS.gene33478.t1 MTR_7g051830 28.947 456 290 8 19 474 10 431 1.76e-51 182
MS.gene33478.t1 MTR_7g052600 28.509 456 292 8 19 474 10 431 3.04e-50 179
MS.gene33478.t1 MTR_1g009170 32.615 325 164 8 157 473 9 286 2.01e-43 158
MS.gene33478.t1 MTR_4g033310 46.524 187 93 2 13 199 10 189 2.27e-43 160
MS.gene33478.t1 MTR_4g033310 38.462 195 112 2 282 474 202 390 1.37e-39 149
MS.gene33478.t1 MTR_3g065350 39.259 135 76 2 340 474 1 129 1.10e-22 95.1
MS.gene33478.t1 MTR_7g064160 34.375 96 63 0 11 106 6 101 1.02e-15 73.9
MS.gene33478.t1 MTR_8g091265 35.238 105 64 2 304 404 5 109 4.97e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33478.t1 AT3G54140 76.724 464 106 2 12 473 2 465 0.0 753
MS.gene33478.t1 AT3G54140 76.724 464 106 2 12 473 2 465 0.0 753
MS.gene33478.t1 AT5G01180 72.903 465 125 1 10 473 1 465 0.0 723
MS.gene33478.t1 AT5G01180 72.903 465 125 1 10 473 1 465 0.0 723
MS.gene33478.t1 AT5G01180 72.903 465 125 1 10 473 1 465 0.0 723
MS.gene33478.t1 AT2G02040 60.256 468 177 6 12 473 19 483 0.0 575
MS.gene33478.t1 AT1G62200 56.549 481 188 7 2 474 23 490 0.0 548
MS.gene33478.t1 AT1G62200 53.529 510 187 9 2 474 37 533 0.0 533
MS.gene33478.t1 AT1G62200 53.529 510 187 9 2 474 23 519 0.0 532
MS.gene33478.t1 AT2G02020 53.105 467 179 6 10 473 18 447 3.12e-173 498
MS.gene33478.t1 AT2G02020 48.394 467 180 8 10 473 18 426 2.40e-147 431
MS.gene33478.t1 AT2G40460 42.489 466 256 5 12 474 2 458 2.89e-129 387
MS.gene33478.t1 AT1G68570 46.507 458 236 5 24 474 15 470 4.57e-127 382
MS.gene33478.t1 AT5G46050 42.373 472 259 5 10 474 3 468 6.02e-127 381
MS.gene33478.t1 AT5G46040 42.585 472 258 5 10 474 3 468 1.01e-126 381
MS.gene33478.t1 AT1G68570 47.477 436 222 4 46 474 5 440 2.31e-125 377
MS.gene33478.t1 AT1G12110 43.696 460 248 7 24 474 17 474 1.29e-121 368
MS.gene33478.t1 AT2G37900 42.797 479 257 8 1 473 1 468 3.05e-121 366
MS.gene33478.t1 AT3G54450 40.929 452 245 6 31 474 9 446 3.08e-118 358
MS.gene33478.t1 AT4G21680 42.308 468 254 8 18 473 12 475 3.86e-117 356
MS.gene33478.t1 AT5G13400 41.239 468 254 8 23 474 52 514 1.07e-116 356
MS.gene33478.t1 AT2G26690 44.255 470 243 8 12 473 2 460 8.50e-116 352
MS.gene33478.t1 AT3G53960 42.266 459 256 8 20 474 23 476 4.86e-115 351
MS.gene33478.t1 AT1G69870 42.070 454 247 10 32 474 49 497 8.48e-115 351
MS.gene33478.t1 AT1G32450 39.875 479 273 7 6 473 12 486 5.79e-112 344
MS.gene33478.t1 AT5G19640 39.501 481 265 7 2 473 22 485 1.03e-111 343
MS.gene33478.t1 AT2G37900 45.113 399 206 7 77 473 2 389 3.15e-107 328
MS.gene33478.t1 AT2G26690 44.725 436 222 8 46 473 3 427 1.07e-106 328
MS.gene33478.t1 AT1G22540 40.466 472 256 7 8 474 4 455 1.61e-106 328
MS.gene33478.t1 AT1G59740 38.806 469 274 5 11 474 18 478 8.79e-106 327
MS.gene33478.t1 AT1G72125 41.053 475 252 10 7 474 6 459 1.01e-105 326
MS.gene33478.t1 AT1G72140 37.161 479 259 6 9 474 6 455 1.01e-103 321
MS.gene33478.t1 AT2G26690 44.706 425 216 8 57 473 2 415 2.44e-103 319
MS.gene33478.t1 AT3G21670 41.822 483 248 10 4 473 2 464 3.61e-103 320
MS.gene33478.t1 AT1G72130 38.495 465 236 10 18 474 14 436 3.20e-102 316
MS.gene33478.t1 AT1G22570 38.797 482 262 8 10 475 3 467 9.15e-102 316
MS.gene33478.t1 AT1G72120 39.009 464 256 6 17 474 13 455 3.33e-101 314
MS.gene33478.t1 AT1G69850 38.763 485 265 8 13 473 4 480 1.01e-98 308
MS.gene33478.t1 AT1G22550 37.860 486 265 8 10 479 3 467 1.44e-95 300
MS.gene33478.t1 AT1G22570 39.414 444 239 7 45 475 4 430 2.76e-94 295
MS.gene33478.t1 AT1G27040 38.429 471 253 10 20 473 9 459 8.98e-93 293
MS.gene33478.t1 AT1G27040 38.429 471 253 10 20 473 13 463 9.55e-93 293
MS.gene33478.t1 AT1G18880 36.093 471 281 11 19 474 4 469 9.71e-93 293
MS.gene33478.t1 AT1G27080 36.819 459 255 10 32 474 12 451 5.98e-92 291
MS.gene33478.t1 AT1G33440 36.287 474 281 7 7 474 11 469 2.29e-90 287
MS.gene33478.t1 AT1G27040 38.889 450 238 10 41 473 2 431 1.65e-89 283
MS.gene33478.t1 AT1G52190 36.522 460 269 10 32 474 19 472 5.87e-89 284
MS.gene33478.t1 AT5G46050 41.713 362 198 5 120 474 1 356 8.01e-89 280
MS.gene33478.t1 AT5G28470 34.812 451 280 7 32 474 15 459 8.22e-86 275
MS.gene33478.t1 AT5G62730 34.783 483 268 8 10 473 32 486 1.42e-84 272
MS.gene33478.t1 AT5G62680 35.608 469 280 11 24 474 31 495 2.45e-84 272
MS.gene33478.t1 AT3G16180 35.622 466 274 10 27 474 15 472 3.16e-84 271
MS.gene33478.t1 AT5G14940 34.273 461 263 10 32 474 5 443 4.62e-83 267
MS.gene33478.t1 AT5G28470 34.590 451 279 8 32 474 15 457 6.45e-83 267
MS.gene33478.t1 AT5G62730 35.541 453 245 8 40 473 3 427 1.39e-80 260
MS.gene33478.t1 AT3G47960 34.624 465 288 9 23 474 50 511 5.44e-80 261
MS.gene33478.t1 AT1G27080 35.915 426 238 10 65 474 3 409 7.70e-80 258
MS.gene33478.t1 AT3G45650 31.897 464 292 8 18 474 6 452 2.40e-75 247
MS.gene33478.t1 AT1G69860 33.983 462 285 9 19 473 4 452 8.56e-75 245
MS.gene33478.t1 AT3G45650 31.897 464 292 8 18 474 6 452 9.02e-75 246
MS.gene33478.t1 AT3G45660 33.100 429 263 8 53 474 40 451 1.44e-72 239
MS.gene33478.t1 AT3G45660 33.100 429 263 8 53 474 40 451 1.44e-72 239
MS.gene33478.t1 AT3G45660 33.100 429 263 8 53 474 61 472 2.38e-72 239
MS.gene33478.t1 AT3G01350 32.906 468 272 11 31 474 4 453 4.31e-72 238
MS.gene33478.t1 AT1G72130 37.500 336 171 7 147 474 14 318 1.11e-71 233
MS.gene33478.t1 AT1G72130 37.500 336 171 7 147 474 14 318 1.11e-71 233
MS.gene33478.t1 AT3G45720 32.629 426 263 10 56 474 41 449 1.97e-67 226
MS.gene33478.t1 AT3G45680 30.472 466 299 10 16 474 4 451 9.37e-67 224
MS.gene33478.t1 AT3G25260 31.223 458 277 7 19 474 6 427 1.02e-64 218
MS.gene33478.t1 AT3G45710 30.263 456 296 9 26 474 13 453 7.73e-64 216
MS.gene33478.t1 AT3G45700 30.490 469 283 14 18 474 4 441 5.61e-63 214
MS.gene33478.t1 AT3G45690 29.295 454 294 10 27 476 13 443 1.86e-62 212
MS.gene33478.t1 AT3G25280 30.856 444 267 7 34 474 21 427 7.89e-60 205
MS.gene33478.t1 AT5G11570 38.618 246 129 8 43 279 22 254 9.69e-43 158
MS.gene33478.t1 AT5G11570 30.968 155 101 2 321 474 237 386 1.67e-15 79.3
MS.gene33478.t1 AT2G38100 27.619 420 272 8 62 475 2 395 5.27e-39 148
MS.gene33478.t1 AT2G38100 27.619 420 272 8 62 475 18 411 1.50e-38 147
MS.gene33478.t1 AT2G38100 27.619 420 272 8 62 475 24 417 1.94e-38 147

Find 115 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGTGTGTTCAAGTTTC+CGG 0.188591 4.3:-83348142 None:intergenic
GTCGGATGGGGATGGGGTTT+TGG 0.221483 4.3:+83347915 MS.gene33478:CDS
ACACTCTAAGTGTCATCTTC+TGG 0.233776 4.3:+83348588 MS.gene33478:CDS
TCAAGTAGAGTGCTATAAAA+AGG 0.241063 4.3:-83347716 None:intergenic
AAACCGTGTGTTTCATCTTT+TGG 0.251095 4.3:+83347762 MS.gene33478:CDS
CAAGTATGCATCAGCTAAAA+AGG 0.280188 4.3:-83346733 None:intergenic
TTTCCCTGATTTCAGGTGTT+TGG 0.304831 4.3:+83348319 MS.gene33478:intron
AAGTTATTGTTGCAGCATTT+AGG 0.328500 4.3:+83348042 MS.gene33478:CDS
CCATCCCTATGTCGATCTTC+TGG 0.340879 4.3:+83348798 MS.gene33478:CDS
AAGCCTTCATGTGATACTAA+TGG 0.350788 4.3:+83347663 MS.gene33478:CDS
TTGCTTCCTCGGTGTTGGTT+TGG 0.353446 4.3:+83347880 MS.gene33478:CDS
GAAGTAAAACCAATTGAAAA+AGG 0.359554 4.3:-83347835 None:intergenic
CACTCTAAGTGTCATCTTCT+GGG 0.360635 4.3:+83348589 MS.gene33478:CDS
CGTTCGTTTACTCCCCGTTT+GGG 0.363000 4.3:+83348439 MS.gene33478:CDS
CTTGAAGACCGTTACGGACT+TGG 0.389786 4.3:+83346642 MS.gene33478:CDS
TCGTTCGTTTACTCCCCGTT+TGG 0.403486 4.3:+83348438 MS.gene33478:CDS
TGATTTCAGGTGTTTGGATA+AGG 0.407783 4.3:+83348325 MS.gene33478:intron
TACTTGATTGCACTTGGAAC+TGG 0.410274 4.3:+83347732 MS.gene33478:CDS
TGCAACAGTATACAGTCAAA+TGG 0.413873 4.3:+83348478 MS.gene33478:CDS
AGCCTTCATGTGATACTAAT+GGG 0.420010 4.3:+83347664 MS.gene33478:CDS
GTCATCTTGATTACTTCTAC+TGG 0.423570 4.3:+83349050 MS.gene33478:CDS
GTTGCAGCATTTAGGAAACT+TGG 0.431571 4.3:+83348050 MS.gene33478:CDS
CGGTGCACTCATTGCTTCCT+CGG 0.439767 4.3:+83347869 MS.gene33478:CDS
ACTTGACAAATCCTTGTAAG+AGG 0.442092 4.3:-83348023 None:intergenic
AAAGCATGCCGCTTTATTCT+CGG 0.445366 4.3:+83346279 MS.gene33478:CDS
TAAAGAAGAGACTTATCATC+TGG 0.447791 4.3:-83348083 None:intergenic
GGCTATCAGTGATGCCCAAA+CGG 0.448610 4.3:-83348453 None:intergenic
ATCATTTCCATGATCGTGGC+CGG 0.449284 4.3:+83348716 MS.gene33478:CDS
ATGAATGTCGGATGGGGATG+GGG 0.453174 4.3:+83347909 MS.gene33478:CDS
ACTCATTGCTTCCTCGGTGT+TGG 0.455639 4.3:+83347875 MS.gene33478:CDS
TAGAATCCCGGCCACGATCA+TGG 0.456374 4.3:-83348723 None:intergenic
GTGCAGAGCCTCCATGGATT+CGG 0.457325 4.3:-83348386 None:intergenic
GCTATCAGTGATGCCCAAAC+GGG 0.459760 4.3:-83348452 None:intergenic
ATGCATACTTGGGAAGATAC+TGG 0.462100 4.3:+83346745 MS.gene33478:CDS
TGACACTTAGAGTGTCGAAA+AGG 0.462376 4.3:-83348580 None:intergenic
TGTTACATCACACCATTGCT+TGG 0.462596 4.3:+83346708 MS.gene33478:CDS
GATGAATGTCGGATGGGGAT+GGG 0.465810 4.3:+83347908 MS.gene33478:CDS
GCTGTGTGAATCCGTGTTCA+TGG 0.471308 4.3:-83348661 None:intergenic
AGATGAATGTCGGATGGGGA+TGG 0.477086 4.3:+83347907 MS.gene33478:CDS
TGTGAAAGATTGGCGTACTA+TGG 0.478383 4.3:+83346594 MS.gene33478:CDS
TTCACACAGCTTCAAAGAAT+CGG 0.484967 4.3:+83348674 MS.gene33478:CDS
GATGGTTTATATACAGAAGA+TGG 0.492031 4.3:+83346204 MS.gene33478:CDS
GGAACCATGTTTGTGTTACA+AGG 0.495089 4.3:+83348500 MS.gene33478:CDS
AATGGGTGCCATCCGACATC+AGG 0.500478 4.3:+83347681 MS.gene33478:CDS
CTATGGCATGAGTACAAACT+TGG 0.503933 4.3:+83346611 MS.gene33478:CDS
CTGCAAATACTGTCACAACT+TGG 0.505268 4.3:+83346676 MS.gene33478:CDS
AAGTGTCATCTTCTGGGCCC+CGG 0.506775 4.3:+83348595 MS.gene33478:CDS
ACGATGAGACGGTCATAGAC+CGG 0.511271 4.3:-83348614 None:intergenic
GCAACAGTATACAGTCAAAT+GGG 0.512639 4.3:+83348479 MS.gene33478:CDS
GAAGATGTCAATCTAAAAGC+TGG 0.513304 4.3:+83346168 MS.gene33478:CDS
ATGTAGAATCTAACATCAAA+GGG 0.513359 4.3:+83348117 MS.gene33478:CDS
GCACTCTACTTGATTGCACT+TGG 0.515219 4.3:+83347726 MS.gene33478:CDS
GACACTTAGAGTGTCGAAAA+GGG 0.515286 4.3:-83348579 None:intergenic
CGGTGAACTTTCTTGCGAAA+GGG 0.518248 4.3:-83348637 None:intergenic
TAAAAGATTTACCGAATCCA+TGG 0.520585 4.3:+83348375 MS.gene33478:CDS
AATACTGTCACAACTTGGTC+GGG 0.527535 4.3:+83346681 MS.gene33478:CDS
CGATGAGACGGTCATAGACC+GGG 0.528157 4.3:-83348613 None:intergenic
GTTACTGTGCAGAGCCTCCA+TGG 0.528525 4.3:-83348392 None:intergenic
AAATGAATGTTGTGAAAGAT+TGG 0.530609 4.3:+83346584 MS.gene33478:CDS
CCGGTGAACTTTCTTGCGAA+AGG 0.533554 4.3:-83348638 None:intergenic
GATGTAGAATCTAACATCAA+AGG 0.533602 4.3:+83348116 MS.gene33478:CDS
TCATTTCCATGATCGTGGCC+GGG 0.533978 4.3:+83348717 MS.gene33478:CDS
TTGCGAAAGGGACGATGAGA+CGG 0.537521 4.3:-83348625 None:intergenic
CTGGTGGAAGTCCTCTTACA+AGG 0.537556 4.3:+83348012 MS.gene33478:CDS
AACTATCTTGAAGACCGTTA+CGG 0.538666 4.3:+83346636 MS.gene33478:CDS
TGGAGTGCCTGGAGTTGCAA+TGG 0.539760 4.3:+83347935 MS.gene33478:CDS
AAAAGGGAGAGGGAGGCTGA+TGG 0.541173 4.3:-83348563 None:intergenic
CAGCTTCAAAGAATCGGAAT+CGG 0.543032 4.3:+83348680 MS.gene33478:CDS
CGGTATAGACTTCAAATACC+TGG 0.543297 4.3:+83347993 MS.gene33478:CDS
AAATACTGTCACAACTTGGT+CGG 0.544661 4.3:+83346680 MS.gene33478:CDS
TTGCAGCATTTAGGAAACTT+GGG 0.546918 4.3:+83348051 MS.gene33478:CDS
AATCTAACATCAAAGGGAGC+CGG 0.551381 4.3:+83348123 MS.gene33478:CDS
TGTGAATCCGTGTTCATGGC+CGG 0.553955 4.3:-83348657 None:intergenic
GCGGATGACTTCTAGAATCC+CGG 0.558196 4.3:-83348735 None:intergenic
GCGATAACCATTGCAACTCC+AGG 0.558801 4.3:-83347942 None:intergenic
CAGTTTGTCCTGATGTCGGA+TGG 0.566763 4.3:-83347689 None:intergenic
TCACATGAAGGCTTTAGTCC+AGG 0.568348 4.3:-83347654 None:intergenic
ACTTGCCAGAAGATCGACAT+AGG 0.569304 4.3:-83348803 None:intergenic
CTTTAGTCCAGGTGCAATTG+CGG 0.570201 4.3:-83347643 None:intergenic
GGATACAGATGAATGTCGGA+TGG 0.573725 4.3:+83347901 MS.gene33478:CDS
TGGAAATGATTGAGATGACT+AGG 0.582009 4.3:-83348703 None:intergenic
TTAGAGTGTCGAAAAGGGAG+AGG 0.583008 4.3:-83348574 None:intergenic
TTGACTGTATACTGTTGCAA+AGG 0.587730 4.3:-83348474 None:intergenic
GCCGCAGTTTGTCCTGATGT+CGG 0.589402 4.3:-83347693 None:intergenic
GATACAGATGAATGTCGGAT+GGG 0.590289 4.3:+83347902 MS.gene33478:CDS
TTGATTGCACTTGGAACTGG+TGG 0.592391 4.3:+83347735 MS.gene33478:CDS
CAGTAGAGACAGAATCCGAC+CGG 0.595827 4.3:+83348351 MS.gene33478:CDS
TAGAGTGTCGAAAAGGGAGA+GGG 0.597883 4.3:-83348573 None:intergenic
CTCAATCATTTCCATGATCG+TGG 0.602413 4.3:+83348712 MS.gene33478:CDS
AGTGTCGAAAAGGGAGAGGG+AGG 0.610825 4.3:-83348570 None:intergenic
CCAGAAGATCGACATAGGGA+TGG 0.612471 4.3:-83348798 None:intergenic
AAGTTCACCGGCCATGAACA+CGG 0.612897 4.3:+83348650 MS.gene33478:CDS
CTTGTAAGAGGACTTCCACC+AGG 0.614606 4.3:-83348011 None:intergenic
CACCCATTAGTATCACATGA+AGG 0.616791 4.3:-83347666 None:intergenic
GCTGGTGTGAATGAAGAAGA+TGG 0.624270 4.3:+83346186 MS.gene33478:CDS
CTGCATTTCCAAGTCCGTAA+CGG 0.628403 4.3:-83346650 None:intergenic
TCCGACATCAGGACAAACTG+CGG 0.633570 4.3:+83347692 MS.gene33478:CDS
ATTTAATACCGAGAATAAAG+CGG 0.636396 4.3:-83346287 None:intergenic
GTACCGCAGTATTTCCTCGT+AGG 0.638440 4.3:+83348824 MS.gene33478:CDS
CCTTTCGCAAGAAAGTTCAC+CGG 0.638836 4.3:+83348638 MS.gene33478:CDS
GTTTGGATACAGATGAATGT+CGG 0.639388 4.3:+83347897 MS.gene33478:CDS
ATACAGATGAATGTCGGATG+GGG 0.656151 4.3:+83347903 MS.gene33478:CDS
CTTGCCAGAAGATCGACATA+GGG 0.656281 4.3:-83348802 None:intergenic
ATGAAAACGACGAGACTGAG+AGG 0.659831 4.3:+83347799 MS.gene33478:CDS
AACACAATGGATCAACACAT+TGG 0.661215 4.3:+83348524 MS.gene33478:CDS
TGTGTTACAAGGCAACACAA+TGG 0.665398 4.3:+83348511 MS.gene33478:CDS
AAACTTCTGCGGCACCTACG+AGG 0.670044 4.3:-83348838 None:intergenic
TATAGACTTCAAATACCTGG+TGG 0.670472 4.3:+83347996 MS.gene33478:CDS
GTTGCCTTGTAACACAAACA+TGG 0.693293 4.3:-83348504 None:intergenic
CTATCAGTGATGCCCAAACG+GGG 0.694144 4.3:-83348451 None:intergenic
GATGAGACGGTCATAGACCG+GGG 0.714307 4.3:-83348612 None:intergenic
AAGATTTACCGAATCCATGG+AGG 0.715910 4.3:+83348378 MS.gene33478:CDS
GCACCAAAAGATGAAACACA+CGG 0.721405 4.3:-83347765 None:intergenic
CTGTATCCAAACCAACACCG+AGG 0.799571 4.3:-83347886 None:intergenic
GCACCTACGAGGAAATACTG+CGG 0.845378 4.3:-83348827 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAAGATAAATATAAAAA+TGG - chr4.3:83348189-83348208 None:intergenic 10.0%
!!! ATTTTCAAGAAATTTTAACT+CGG + chr4.3:83347425-83347444 MS.gene33478:intron 15.0%
!!! TTGTTAAAATGTTTTGATAA+AGG + chr4.3:83346832-83346851 MS.gene33478:intron 15.0%
!!! TTTTACTTCTCAATTAATAT+CGG + chr4.3:83347849-83347868 MS.gene33478:CDS 15.0%
!! TACATTAAAAGTTAATTGAC+AGG - chr4.3:83347578-83347597 None:intergenic 20.0%
!!! TTACTTGAATTTCAAGTAAA+CGG - chr4.3:83347059-83347078 None:intergenic 20.0%
!!! TTTTCAAGAAATTTTAACTC+GGG + chr4.3:83347426-83347445 MS.gene33478:intron 20.0%
! AAATGAATGTTGTGAAAGAT+TGG + chr4.3:83346584-83346603 MS.gene33478:CDS 25.0%
! ACAAAAGTTACAACAAGAAA+TGG + chr4.3:83348992-83349011 MS.gene33478:intron 25.0%
! ACCGATGAAGAAAAATATAA+TGG - chr4.3:83347967-83347986 None:intergenic 25.0%
! AGTTCATATTTAAACATCTG+AGG + chr4.3:83346882-83346901 MS.gene33478:intron 25.0%
! ATGTAGAATCTAACATCAAA+GGG + chr4.3:83348117-83348136 MS.gene33478:CDS 25.0%
! ATTCCAGATAATATGAATAG+GGG + chr4.3:83349028-83349047 MS.gene33478:intron 25.0%
! ATTTAATACCGAGAATAAAG+CGG - chr4.3:83346290-83346309 None:intergenic 25.0%
! GAAGTAAAACCAATTGAAAA+AGG - chr4.3:83347838-83347857 None:intergenic 25.0%
! GATTCCAGATAATATGAATA+GGG + chr4.3:83349027-83349046 MS.gene33478:intron 25.0%
! TCAAATAAGTCAATCAAAAC+AGG - chr4.3:83347492-83347511 None:intergenic 25.0%
! TCATATCTGATATATATTCC+TGG + chr4.3:83346342-83346361 MS.gene33478:intron 25.0%
!! ACGAAGTTTAGAATAAGAAA+AGG - chr4.3:83348231-83348250 None:intergenic 25.0%
!! GCCATTATATTTTTCTTCAT+CGG + chr4.3:83347963-83347982 MS.gene33478:CDS 25.0%
!!! GTTTTTGAAGTACATGAAAA+TGG + chr4.3:83347302-83347321 MS.gene33478:intron 25.0%
AAACAAATTTCCCTGATTTC+AGG + chr4.3:83348312-83348331 MS.gene33478:intron 30.0%
AACAATTCTTGTCCTAGAAT+TGG + chr4.3:83346523-83346542 MS.gene33478:intron 30.0%
AAGTTATTGTTGCAGCATTT+AGG + chr4.3:83348042-83348061 MS.gene33478:CDS 30.0%
AATTGTTAGAACAACAATGC+TGG - chr4.3:83346510-83346529 None:intergenic 30.0%
ACAAGAAAAAGACAGGAAAT+TGG + chr4.3:83346256-83346275 MS.gene33478:CDS 30.0%
AGTTCAAAAGAATACACTGA+TGG - chr4.3:83346993-83347012 None:intergenic 30.0%
CAGATCATTAGTATCAAGTT+AGG + chr4.3:83347465-83347484 MS.gene33478:intron 30.0%
GATATTAGTGAAAACTTCTG+CGG - chr4.3:83348852-83348871 None:intergenic 30.0%
GATGGTTTATATACAGAAGA+TGG + chr4.3:83346204-83346223 MS.gene33478:CDS 30.0%
GATGTAGAATCTAACATCAA+AGG + chr4.3:83348116-83348135 MS.gene33478:CDS 30.0%
GCTTGAATAATAGAAATGTG+CGG + chr4.3:83346405-83346424 MS.gene33478:intron 30.0%
GGAAAATGAAAAAATGTTGC+AGG + chr4.3:83346561-83346580 MS.gene33478:intron 30.0%
GGATTCCAGATAATATGAAT+AGG + chr4.3:83349026-83349045 MS.gene33478:intron 30.0%
TAAAAGATTTACCGAATCCA+TGG + chr4.3:83348375-83348394 MS.gene33478:CDS 30.0%
TAAAGAAGAGACTTATCATC+TGG - chr4.3:83348086-83348105 None:intergenic 30.0%
TCCTCAAAGTAGAATAGAAT+TGG - chr4.3:83347330-83347349 None:intergenic 30.0%
! ATTTCAAGTAAACGGTTCAT+TGG - chr4.3:83347051-83347070 None:intergenic 30.0%
! ATTTCCTGTCTTTTTCTTGT+TGG - chr4.3:83346256-83346275 None:intergenic 30.0%
! CAAGTAGAGTGCTATAAAAA+GGG - chr4.3:83347718-83347737 None:intergenic 30.0%
! GCAGAAGTTTTCACTAATAT+CGG + chr4.3:83348851-83348870 MS.gene33478:intron 30.0%
! GCTGATGGAATTTTGAATTT+AGG - chr4.3:83348551-83348570 None:intergenic 30.0%
! TCAAGTAGAGTGCTATAAAA+AGG - chr4.3:83347719-83347738 None:intergenic 30.0%
!! GATTCGGTAAATCTTTTATC+CGG - chr4.3:83348373-83348392 None:intergenic 30.0%
!!! AGAAAAGCTCCTTTTTCAAT+TGG + chr4.3:83347826-83347845 MS.gene33478:CDS 30.0%
!!! GATCATAGTAGTTGTTTTTG+CGG - chr4.3:83348772-83348791 None:intergenic 30.0%
!!! TCTTAGCTCTAGTTTCATTT+TGG + chr4.3:83347364-83347383 MS.gene33478:intron 30.0%
!!! TTTTTAGCTGATGCATACTT+GGG + chr4.3:83346735-83346754 MS.gene33478:CDS 30.0%
AAATACTGTCACAACTTGGT+CGG + chr4.3:83346680-83346699 MS.gene33478:CDS 35.0%
AACACAATGGATCAACACAT+TGG + chr4.3:83348524-83348543 MS.gene33478:CDS 35.0%
AACTATCTTGAAGACCGTTA+CGG + chr4.3:83346636-83346655 MS.gene33478:CDS 35.0%
AAGCCTTCATGTGATACTAA+TGG + chr4.3:83347663-83347682 MS.gene33478:CDS 35.0%
AATTGGATCACACTAAGATG+TGG + chr4.3:83346540-83346559 MS.gene33478:intron 35.0%
ACAACAAGAAATGGAAGTCT+TGG + chr4.3:83349001-83349020 MS.gene33478:intron 35.0%
ACTTGACAAATCCTTGTAAG+AGG - chr4.3:83348026-83348045 None:intergenic 35.0%
AGCCTTCATGTGATACTAAT+GGG + chr4.3:83347664-83347683 MS.gene33478:CDS 35.0%
ATCTTTATGAGACTACTCGT+TGG + chr4.3:83348200-83348219 MS.gene33478:intron 35.0%
CAAGTATGCATCAGCTAAAA+AGG - chr4.3:83346736-83346755 None:intergenic 35.0%
CACCTTGTGCTTAAATACTA+CGG - chr4.3:83346369-83346388 None:intergenic 35.0%
CGATAGAGAAGAGAGATTAA+AGG - chr4.3:83347242-83347261 None:intergenic 35.0%
GAAGATGTCAATCTAAAAGC+TGG + chr4.3:83346168-83346187 MS.gene33478:CDS 35.0%
GCAACAGTATACAGTCAAAT+GGG + chr4.3:83348479-83348498 MS.gene33478:CDS 35.0%
GCCAATTCTATTCTACTTTG+AGG + chr4.3:83347326-83347345 MS.gene33478:intron 35.0%
GTCATCTTGATTACTTCTAC+TGG + chr4.3:83349050-83349069 MS.gene33478:CDS 35.0%
GTTTGGATACAGATGAATGT+CGG + chr4.3:83347897-83347916 MS.gene33478:CDS 35.0%
TATAGACTTCAAATACCTGG+TGG + chr4.3:83347996-83348015 MS.gene33478:CDS 35.0%
TATCCAAACACCTGAAATCA+GGG - chr4.3:83348325-83348344 None:intergenic 35.0%
TCACTCACAACTAATGCATT+GGG + chr4.3:83348935-83348954 MS.gene33478:intron 35.0%
TCTTAGTGTGATCCAATTCT+AGG - chr4.3:83346538-83346557 None:intergenic 35.0%
TGACCCCTATTCATATTATC+TGG - chr4.3:83349034-83349053 None:intergenic 35.0%
TGCAACAGTATACAGTCAAA+TGG + chr4.3:83348478-83348497 MS.gene33478:CDS 35.0%
TGGAAATGATTGAGATGACT+AGG - chr4.3:83348706-83348725 None:intergenic 35.0%
TTATCCAAACACCTGAAATC+AGG - chr4.3:83348326-83348345 None:intergenic 35.0%
TTCACACAGCTTCAAAGAAT+CGG + chr4.3:83348674-83348693 MS.gene33478:CDS 35.0%
TTCACTCACAACTAATGCAT+TGG + chr4.3:83348934-83348953 MS.gene33478:intron 35.0%
TTGACTGTATACTGTTGCAA+AGG - chr4.3:83348477-83348496 None:intergenic 35.0%
TTGCAGCATTTAGGAAACTT+GGG + chr4.3:83348051-83348070 MS.gene33478:CDS 35.0%
TTTCACTAATATCGGTCAGA+TGG + chr4.3:83348859-83348878 MS.gene33478:intron 35.0%
! AAACCGTGTGTTTCATCTTT+TGG + chr4.3:83347762-83347781 MS.gene33478:CDS 35.0%
! ATCTGAGGTTCATAGTCAAA+AGG + chr4.3:83346897-83346916 MS.gene33478:intron 35.0%
! CATTTGTGTGTTCAAGTTTC+CGG - chr4.3:83348145-83348164 None:intergenic 35.0%
! TGATTTCAGGTGTTTGGATA+AGG + chr4.3:83348325-83348344 MS.gene33478:intron 35.0%
! TTTTCTTCATCGGTAGTAGA+CGG + chr4.3:83347973-83347992 MS.gene33478:CDS 35.0%
!! TCTCGTCGTTTTCATCAAAT+TGG - chr4.3:83347794-83347813 None:intergenic 35.0%
!!! CTTTTTAGCTGATGCATACT+TGG + chr4.3:83346734-83346753 MS.gene33478:CDS 35.0%
AAGATTTACCGAATCCATGG+AGG + chr4.3:83348378-83348397 MS.gene33478:CDS 40.0%
AATACTGTCACAACTTGGTC+GGG + chr4.3:83346681-83346700 MS.gene33478:CDS 40.0%
AATCTAACATCAAAGGGAGC+CGG + chr4.3:83348123-83348142 MS.gene33478:CDS 40.0%
ACACTCTAAGTGTCATCTTC+TGG + chr4.3:83348588-83348607 MS.gene33478:CDS 40.0%
ATACAGATGAATGTCGGATG+GGG + chr4.3:83347903-83347922 MS.gene33478:CDS 40.0%
ATGCATACTTGGGAAGATAC+TGG + chr4.3:83346745-83346764 MS.gene33478:CDS 40.0%
CAAGAAATGGAAGTCTTGGT+TGG + chr4.3:83349005-83349024 MS.gene33478:intron 40.0%
CACAAACTTCTCATAGCATC+AGG - chr4.3:83348905-83348924 None:intergenic 40.0%
CACCCATTAGTATCACATGA+AGG - chr4.3:83347669-83347688 None:intergenic 40.0%
CACTCTAAGTGTCATCTTCT+GGG + chr4.3:83348589-83348608 MS.gene33478:CDS 40.0%
CAGCTTCAAAGAATCGGAAT+CGG + chr4.3:83348680-83348699 MS.gene33478:CDS 40.0%
CGGTATAGACTTCAAATACC+TGG + chr4.3:83347993-83348012 MS.gene33478:CDS 40.0%
CGTTGTTTGTGTACTTTCCA+GGG + chr4.3:83347600-83347619 MS.gene33478:intron 40.0%
CTATGGCATGAGTACAAACT+TGG + chr4.3:83346611-83346630 MS.gene33478:CDS 40.0%
CTCAATCATTTCCATGATCG+TGG + chr4.3:83348712-83348731 MS.gene33478:CDS 40.0%
CTGCAAATACTGTCACAACT+TGG + chr4.3:83346676-83346695 MS.gene33478:CDS 40.0%
GACCGTAGTATTTAAGCACA+AGG + chr4.3:83346364-83346383 MS.gene33478:intron 40.0%
GATACAGATGAATGTCGGAT+GGG + chr4.3:83347902-83347921 MS.gene33478:CDS 40.0%
GCACCAAAAGATGAAACACA+CGG - chr4.3:83347768-83347787 None:intergenic 40.0%
GGAACCATGTTTGTGTTACA+AGG + chr4.3:83348500-83348519 MS.gene33478:CDS 40.0%
GTTGCAGCATTTAGGAAACT+TGG + chr4.3:83348050-83348069 MS.gene33478:CDS 40.0%
GTTGCCTTGTAACACAAACA+TGG - chr4.3:83348507-83348526 None:intergenic 40.0%
TACTTGATTGCACTTGGAAC+TGG + chr4.3:83347732-83347751 MS.gene33478:CDS 40.0%
TCGTTGTTTGTGTACTTTCC+AGG + chr4.3:83347599-83347618 MS.gene33478:intron 40.0%
TGTGAAAGATTGGCGTACTA+TGG + chr4.3:83346594-83346613 MS.gene33478:CDS 40.0%
TGTGTTACAAGGCAACACAA+TGG + chr4.3:83348511-83348530 MS.gene33478:CDS 40.0%
TGTTACATCACACCATTGCT+TGG + chr4.3:83346708-83346727 MS.gene33478:CDS 40.0%
TTTGTGTACTTTCCAGGGAA+TGG + chr4.3:83347605-83347624 MS.gene33478:intron 40.0%
! AGTTGTTTCCATAAGCACTC+CGG - chr4.3:83347550-83347569 None:intergenic 40.0%
! CGGTAAATCTTTTATCCGGT+CGG - chr4.3:83348369-83348388 None:intergenic 40.0%
! GATGGAGTTCTTTTATGGTC+AGG + chr4.3:83348877-83348896 MS.gene33478:intron 40.0%
! GGTCAGATGGAGTTCTTTTA+TGG + chr4.3:83348872-83348891 MS.gene33478:intron 40.0%
! TTTCCCTGATTTCAGGTGTT+TGG + chr4.3:83348319-83348338 MS.gene33478:intron 40.0%
!! AAAGCATGCCGCTTTATTCT+CGG + chr4.3:83346279-83346298 MS.gene33478:CDS 40.0%
!! ATGTTAAAAGTGCCATTCCC+TGG - chr4.3:83347620-83347639 None:intergenic 40.0%
!! GACACTTAGAGTGTCGAAAA+GGG - chr4.3:83348582-83348601 None:intergenic 40.0%
!! TGACACTTAGAGTGTCGAAA+AGG - chr4.3:83348583-83348602 None:intergenic 40.0%
AAGCATCTGTGAGACTGTTC+CGG + chr4.3:83347528-83347547 MS.gene33478:intron 45.0%
ACTTGCCAGAAGATCGACAT+AGG - chr4.3:83348806-83348825 None:intergenic 45.0%
ATCATTTCCATGATCGTGGC+CGG + chr4.3:83348716-83348735 MS.gene33478:CDS 45.0%
ATGAAAACGACGAGACTGAG+AGG + chr4.3:83347799-83347818 MS.gene33478:CDS 45.0%
CCTGCCAACAAGAAAAAGAC+AGG + chr4.3:83346249-83346268 MS.gene33478:CDS 45.0%
CCTTTCGCAAGAAAGTTCAC+CGG + chr4.3:83348638-83348657 MS.gene33478:CDS 45.0%
CGGTGAACTTTCTTGCGAAA+GGG - chr4.3:83348640-83348659 None:intergenic 45.0%
CTCGTAGACATAGGCACAAT+TGG + chr4.3:83346441-83346460 MS.gene33478:intron 45.0%
CTGCATTTCCAAGTCCGTAA+CGG - chr4.3:83346653-83346672 None:intergenic 45.0%
CTTGCCAGAAGATCGACATA+GGG - chr4.3:83348805-83348824 None:intergenic 45.0%
CTTTAGTCCAGGTGCAATTG+CGG - chr4.3:83347646-83347665 None:intergenic 45.0%
GAATTGTACTCTCGACAGAC+AGG - chr4.3:83347021-83347040 None:intergenic 45.0%
GCACTCTACTTGATTGCACT+TGG + chr4.3:83347726-83347745 MS.gene33478:CDS 45.0%
GCTAAAAAGGCTCCAAGCAA+TGG - chr4.3:83346723-83346742 None:intergenic 45.0%
GCTGGTGTGAATGAAGAAGA+TGG + chr4.3:83346186-83346205 MS.gene33478:CDS 45.0%
GGATACAGATGAATGTCGGA+TGG + chr4.3:83347901-83347920 MS.gene33478:CDS 45.0%
GTGCTTAAATACTACGGTCC+AGG - chr4.3:83346363-83346382 None:intergenic 45.0%
TTGATTGCACTTGGAACTGG+TGG + chr4.3:83347735-83347754 MS.gene33478:CDS 45.0%
! ACATTTTCCGCAATTGCACC+TGG + chr4.3:83347636-83347655 MS.gene33478:CDS 45.0%
! CCTGTCTTTTTCTTGTTGGC+AGG - chr4.3:83346252-83346271 None:intergenic 45.0%
! TCACATGAAGGCTTTAGTCC+AGG - chr4.3:83347657-83347676 None:intergenic 45.0%
!! TAGAGTGTCGAAAAGGGAGA+GGG - chr4.3:83348576-83348595 None:intergenic 45.0%
!! TGTTTTTGCGGACAATGTCG+AGG - chr4.3:83348760-83348779 None:intergenic 45.0%
!! TTAGAGTGTCGAAAAGGGAG+AGG - chr4.3:83348577-83348596 None:intergenic 45.0%
AAGTTCACCGGCCATGAACA+CGG + chr4.3:83348650-83348669 MS.gene33478:CDS 50.0%
ACGATGAGACGGTCATAGAC+CGG - chr4.3:83348617-83348636 None:intergenic 50.0%
AGATGAATGTCGGATGGGGA+TGG + chr4.3:83347907-83347926 MS.gene33478:CDS 50.0%
ATGAATGTCGGATGGGGATG+GGG + chr4.3:83347909-83347928 MS.gene33478:CDS 50.0%
CACTCTGCACTCGTAGACAT+AGG + chr4.3:83346432-83346451 MS.gene33478:intron 50.0%
CAGTAGAGACAGAATCCGAC+CGG + chr4.3:83348351-83348370 MS.gene33478:CDS 50.0%
CAGTTTGTCCTGATGTCGGA+TGG - chr4.3:83347692-83347711 None:intergenic 50.0%
CCAGAAGATCGACATAGGGA+TGG - chr4.3:83348801-83348820 None:intergenic 50.0%
CCATCCCTATGTCGATCTTC+TGG + chr4.3:83348798-83348817 MS.gene33478:CDS 50.0%
CCGGTGAACTTTCTTGCGAA+AGG - chr4.3:83348641-83348660 None:intergenic 50.0%
CGTTCGTTTACTCCCCGTTT+GGG + chr4.3:83348439-83348458 MS.gene33478:CDS 50.0%
CTATCAGTGATGCCCAAACG+GGG - chr4.3:83348454-83348473 None:intergenic 50.0%
CTATGTCTACGAGTGCAGAG+TGG - chr4.3:83346434-83346453 None:intergenic 50.0%
CTGGTGGAAGTCCTCTTACA+AGG + chr4.3:83348012-83348031 MS.gene33478:CDS 50.0%
CTGTATCCAAACCAACACCG+AGG - chr4.3:83347889-83347908 None:intergenic 50.0%
CTTGAAGACCGTTACGGACT+TGG + chr4.3:83346642-83346661 MS.gene33478:CDS 50.0%
CTTGTAAGAGGACTTCCACC+AGG - chr4.3:83348014-83348033 None:intergenic 50.0%
GATGAATGTCGGATGGGGAT+GGG + chr4.3:83347908-83347927 MS.gene33478:CDS 50.0%
GCACCTACGAGGAAATACTG+CGG - chr4.3:83348830-83348849 None:intergenic 50.0%
GCGATAACCATTGCAACTCC+AGG - chr4.3:83347945-83347964 None:intergenic 50.0%
GCTATCAGTGATGCCCAAAC+GGG - chr4.3:83348455-83348474 None:intergenic 50.0%
GCTGTGTGAATCCGTGTTCA+TGG - chr4.3:83348664-83348683 None:intergenic 50.0%
GGCTATCAGTGATGCCCAAA+CGG - chr4.3:83348456-83348475 None:intergenic 50.0%
GTACCGCAGTATTTCCTCGT+AGG + chr4.3:83348824-83348843 MS.gene33478:CDS 50.0%
TCATTTCCATGATCGTGGCC+GGG + chr4.3:83348717-83348736 MS.gene33478:CDS 50.0%
TCCGACATCAGGACAAACTG+CGG + chr4.3:83347692-83347711 MS.gene33478:CDS 50.0%
TCGTTCGTTTACTCCCCGTT+TGG + chr4.3:83348438-83348457 MS.gene33478:CDS 50.0%
TCTTGCACACACACGCACTT+TGG - chr4.3:83347269-83347288 None:intergenic 50.0%
TGTGAATCCGTGTTCATGGC+CGG - chr4.3:83348660-83348679 None:intergenic 50.0%
TTGCGAAAGGGACGATGAGA+CGG - chr4.3:83348628-83348647 None:intergenic 50.0%
! ACTCATTGCTTCCTCGGTGT+TGG + chr4.3:83347875-83347894 MS.gene33478:CDS 50.0%
! AGACTGTTCCGGAGTGCTTA+TGG + chr4.3:83347539-83347558 MS.gene33478:intron 50.0%
! TTTTGCGGACAATGTCGAGG+CGG - chr4.3:83348757-83348776 None:intergenic 50.0%
!! GCGGATGACTTCTAGAATCC+CGG - chr4.3:83348738-83348757 None:intergenic 50.0%
!! TTGCTTCCTCGGTGTTGGTT+TGG + chr4.3:83347880-83347899 MS.gene33478:CDS 50.0%
AAAAGGGAGAGGGAGGCTGA+TGG - chr4.3:83348566-83348585 None:intergenic 55.0%
AAACTTCTGCGGCACCTACG+AGG - chr4.3:83348841-83348860 None:intergenic 55.0%
AAGTGTCATCTTCTGGGCCC+CGG + chr4.3:83348595-83348614 MS.gene33478:CDS 55.0%
CGATGAGACGGTCATAGACC+GGG - chr4.3:83348616-83348635 None:intergenic 55.0%
CGGTGCACTCATTGCTTCCT+CGG + chr4.3:83347869-83347888 MS.gene33478:CDS 55.0%
GATGAGACGGTCATAGACCG+GGG - chr4.3:83348615-83348634 None:intergenic 55.0%
GCCGCAGTTTGTCCTGATGT+CGG - chr4.3:83347696-83347715 None:intergenic 55.0%
GTGCAGAGCCTCCATGGATT+CGG - chr4.3:83348389-83348408 None:intergenic 55.0%
GTTACTGTGCAGAGCCTCCA+TGG - chr4.3:83348395-83348414 None:intergenic 55.0%
TAGAATCCCGGCCACGATCA+TGG - chr4.3:83348726-83348745 None:intergenic 55.0%
! AATGGGTGCCATCCGACATC+AGG + chr4.3:83347681-83347700 MS.gene33478:CDS 55.0%
! AGTGTCGAAAAGGGAGAGGG+AGG - chr4.3:83348573-83348592 None:intergenic 55.0%
! TGGAGTGCCTGGAGTTGCAA+TGG + chr4.3:83347935-83347954 MS.gene33478:CDS 55.0%
! GTCGGATGGGGATGGGGTTT+TGG + chr4.3:83347915-83347934 MS.gene33478:CDS 60.0%
!!! GGATGGGGTTTTGGAGTGCC+TGG + chr4.3:83347924-83347943 MS.gene33478:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 83346165 83349079 83346165 ID=MS.gene33478
chr4.3 mRNA 83346165 83349079 83346165 ID=MS.gene33478.t1;Parent=MS.gene33478
chr4.3 exon 83346165 83346300 83346165 ID=MS.gene33478.t1.exon1;Parent=MS.gene33478.t1
chr4.3 CDS 83346165 83346300 83346165 ID=cds.MS.gene33478.t1;Parent=MS.gene33478.t1
chr4.3 exon 83346583 83346800 83346583 ID=MS.gene33478.t1.exon2;Parent=MS.gene33478.t1
chr4.3 CDS 83346583 83346800 83346583 ID=cds.MS.gene33478.t1;Parent=MS.gene33478.t1
chr4.3 exon 83347621 83348171 83347621 ID=MS.gene33478.t1.exon3;Parent=MS.gene33478.t1
chr4.3 CDS 83347621 83348171 83347621 ID=cds.MS.gene33478.t1;Parent=MS.gene33478.t1
chr4.3 exon 83348334 83348845 83348334 ID=MS.gene33478.t1.exon4;Parent=MS.gene33478.t1
chr4.3 CDS 83348334 83348845 83348334 ID=cds.MS.gene33478.t1;Parent=MS.gene33478.t1
chr4.3 exon 83349048 83349079 83349048 ID=MS.gene33478.t1.exon5;Parent=MS.gene33478.t1
chr4.3 CDS 83349048 83349079 83349048 ID=cds.MS.gene33478.t1;Parent=MS.gene33478.t1
Gene Sequence

>MS.gene33478

ATGGAAGATGTCAATCTAAAAGCTGGTGTGAATGAAGAAGATGGTTTATATACAGAAGATGGAACAATTGACATTCACAAAAATCCTGCCAACAAGAAAAAGACAGGAAATTGGAAAGCATGCCGCTTTATTCTCGGTATTAAATTATTAGTTTTATATCTTTCATAAATTTCAATGTCATATCTGATATATATTCCTGGACCGTAGTATTTAAGCACAAGGTGTACTTTGTAACTCTAAGCTTGAATAATAGAAATGTGCGGCAACCACTCTGCACTCGTAGACATAGGCACAATTGGTTGCGTGTTCTCTGTTTGCTTTTCTAGTATACTTACTCTTCAACCAGCATTGTTGTTCTAACAATTCTTGTCCTAGAATTGGATCACACTAAGATGTGGAAAATGAAAAAATGTTGCAGGAAATGAATGTTGTGAAAGATTGGCGTACTATGGCATGAGTACAAACTTGGTGAACTATCTTGAAGACCGTTACGGACTTGGAAATGCAGCTGCTGCAAATACTGTCACAACTTGGTCGGGTACATGTTACATCACACCATTGCTTGGAGCCTTTTTAGCTGATGCATACTTGGGAAGATACTGGACAATTGCTAGTTTCTCGAGTATCTATGTCATTGTAAGCTCATTTTAAGATAAGATTTGCTTTTTTGTTAAAATGTTTTGATAAAGGCGTTTAATATGCATTCTTTTGAATATCAGTTCATATTTAAACATCTGAGGTTCATAGTCAAAAGGATCGATACTTTTAATTAACAATACGTTTCTTTTTTAATCAAGTAAAATATTGTTAATTTTGTCTATTTTTCCATCAGTGTATTCTTTTGAACTGAAGTCCTGTCTGTCGAGAGTACAATTCAAATTAGCCAATGAACCGTTTACTTGAAATTCAAGTAACTGAAATGCTGAATTTTCTGTTTTCGTTAGATAAGCAGTTATTTTCTGTTATGTGTGTGATGTCTGTGAGTCATCATCGATCATATGTGTATAAATACTTGATGTGAATTATACTCTCGACAACTAGTGATTTTCTCTCAATTTTCTCTATTCTTTCTCTCCTTTAATCTCTCTTCTCTATCGATAACCAAAGTGCGTGTGTGTGCAAGAAAAACATCAGTGAGTTTTTGAAGTACATGAAAATGGTGCCAATTCTATTCTACTTTGAGGACAGTATAGATTTAATCTTAGCTCTAGTTTCATTTTGGAATCTTCAGTTTACTTCTAAACATTTACTGCATTTCAAATTTTCAAGAAATTTTAACTCGGGTACTTAAACTGTGTTTCAGATCATTAGTATCAAGTTAGGACCTGTTTTGATTGACTTATTTGAGTTTATCTACACGCATAAGCATCTGTGAGACTGTTCCGGAGTGCTTATGGAAACAACTCATGACCTGTCAATTAACTTTTAATGTAGTCGTTGTTTGTGTACTTTCCAGGGAATGGCACTTTTAACATTTTCCGCAATTGCACCTGGACTAAAGCCTTCATGTGATACTAATGGGTGCCATCCGACATCAGGACAAACTGCGGCCCTTTTTATAGCACTCTACTTGATTGCACTTGGAACTGGTGGTATTAAACCGTGTGTTTCATCTTTTGGTGCTGACCAATTTGATGAAAACGACGAGACTGAGAGGAAAAAGAAAAGCTCCTTTTTCAATTGGTTTTACTTCTCAATTAATATCGGTGCACTCATTGCTTCCTCGGTGTTGGTTTGGATACAGATGAATGTCGGATGGGGATGGGGTTTTGGAGTGCCTGGAGTTGCAATGGTTATCGCCATTATATTTTTCTTCATCGGTAGTAGACGGTATAGACTTCAAATACCTGGTGGAAGTCCTCTTACAAGGATTTGTCAAGTTATTGTTGCAGCATTTAGGAAACTTGGGCAACAAGTTCCAGATGATAAGTCTCTTCTTTATGAGACTACTGATGTAGAATCTAACATCAAAGGGAGCCGGAAACTTGAACACACAAATGAATTGAAGTAAGATATCAAAACCATTTTTATATTTATCTTTATGAGACTACTCGTTGGCATGTCCTTTTCTTATTCTAAACTTCGTAATTCACTAACGCTAAAGTTTGTTTTATACATTCTTATCTTACATTATAATAATAACTAATAAACAAATTTCCCTGATTTCAGGTGTTTGGATAAGGCAGCAGTAGAGACAGAATCCGACCGGATAAAAGATTTACCGAATCCATGGAGGCTCTGCACAGTAACACAAGTCGAAGAACTCAAATCAATCGTTCGTTTACTCCCCGTTTGGGCATCACTGATAGCCTTTGCAACAGTATACAGTCAAATGGGAACCATGTTTGTGTTACAAGGCAACACAATGGATCAACACATTGGTCCTAAATTCAAAATTCCATCAGCCTCCCTCTCCCTTTTCGACACTCTAAGTGTCATCTTCTGGGCCCCGGTCTATGACCGTCTCATCGTCCCTTTCGCAAGAAAGTTCACCGGCCATGAACACGGATTCACACAGCTTCAAAGAATCGGAATCGGCCTAGTCATCTCAATCATTTCCATGATCGTGGCCGGGATTCTAGAAGTCATCCGCCTCGACATTGTCCGCAAAAACAACTACTATGATCTCGAAACCATCCCTATGTCGATCTTCTGGCAAGTACCGCAGTATTTCCTCGTAGGTGCCGCAGAAGTTTTCACTAATATCGGTCAGATGGAGTTCTTTTATGGTCAGGCACCTGATGCTATGAGAAGTTTGTGTGCAGCACTTTCACTCACAACTAATGCATTGGGAAATTATGTGAGTACATTGCTTGTTACTATTGTCACAAAAGTTACAACAAGAAATGGAAGTCTTGGTTGGATTCCAGATAATATGAATAGGGGTCATCTTGATTACTTCTACTGGCTTTTAA
Protein sequence

>MS.gene33478.t1

MEDVNLKAGVNEEDGLYTEDGTIDIHKNPANKKKTGNWKACRFILGNECCERLAYYGMSTNLVNYLEDRYGLGNAAAANTVTTWSGTCYITPLLGAFLADAYLGRYWTIASFSSIYVIGMALLTFSAIAPGLKPSCDTNGCHPTSGQTAALFIALYLIALGTGGIKPCVSSFGADQFDENDETERKKKSSFFNWFYFSINIGALIASSVLVWIQMNVGWGWGFGVPGVAMVIAIIFFFIGSRRYRLQIPGGSPLTRICQVIVAAFRKLGQQVPDDKSLLYETTDVESNIKGSRKLEHTNELKCLDKAAVETESDRIKDLPNPWRLCTVTQVEELKSIVRLLPVWASLIAFATVYSQMGTMFVLQGNTMDQHIGPKFKIPSASLSLFDTLSVIFWAPVYDRLIVPFARKFTGHEHGFTQLQRIGIGLVISIISMIVAGILEVIRLDIVRKNNYYDLETIPMSIFWQVPQYFLVGVILITSTGF