AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052754

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052754.t1 MTR_4g015080 97.040 473 14 0 1 473 1 473 0.0 959
MS.gene052754.t1 MTR_7g067010 83.948 461 74 0 13 473 3 463 0.0 822
MS.gene052754.t1 MTR_3g462710 69.828 464 138 2 12 473 2 465 0.0 670
MS.gene052754.t1 MTR_3g462710 70.506 356 103 2 120 473 1 356 3.50e-179 510
MS.gene052754.t1 MTR_3g112470 47.845 464 229 5 13 473 2 455 1.18e-148 436
MS.gene052754.t1 MTR_3g112460 47.845 464 229 5 13 473 2 455 1.37e-148 436
MS.gene052754.t1 MTR_7g098200 44.043 470 248 5 12 474 9 470 1.87e-140 417
MS.gene052754.t1 MTR_3g112450 46.930 456 226 6 23 473 12 456 3.25e-137 407
MS.gene052754.t1 MTR_7g098180 43.154 482 257 7 1 474 1 473 7.76e-136 405
MS.gene052754.t1 MTR_7g098200 43.830 470 239 7 12 474 9 460 1.68e-135 404
MS.gene052754.t1 MTR_7g098220 44.350 469 248 3 13 474 9 471 9.86e-135 402
MS.gene052754.t1 MTR_3g072300 44.206 466 252 5 12 474 4 464 2.78e-134 400
MS.gene052754.t1 MTR_7g098160 41.494 482 266 5 1 474 1 474 1.70e-132 396
MS.gene052754.t1 MTR_7g098040 42.324 482 262 5 1 474 2 475 3.48e-132 396
MS.gene052754.t1 MTR_5g055000 45.111 450 237 6 32 474 16 462 7.95e-132 394
MS.gene052754.t1 MTR_5g012290 45.534 459 240 7 24 473 17 474 1.19e-129 389
MS.gene052754.t1 MTR_7g088825 41.949 472 264 5 10 474 1 469 1.44e-129 389
MS.gene052754.t1 MTR_1g075440 43.856 472 247 7 10 473 7 468 2.12e-129 388
MS.gene052754.t1 MTR_7g098090 41.068 487 266 6 1 474 1 479 6.28e-129 387
MS.gene052754.t1 MTR_7g098150 41.453 468 258 5 1 460 1 460 2.70e-128 382
MS.gene052754.t1 MTR_7g098230 44.191 439 232 4 43 474 35 467 1.65e-125 378
MS.gene052754.t1 MTR_7g010820 43.515 478 249 6 10 473 2 472 1.19e-124 375
MS.gene052754.t1 MTR_1g041875 42.038 471 253 6 13 474 4 463 3.24e-124 374
MS.gene052754.t1 MTR_4g101380 43.939 462 246 6 24 473 17 477 5.45e-124 374
MS.gene052754.t1 MTR_1g041835 43.629 463 249 5 17 473 10 466 4.42e-123 371
MS.gene052754.t1 MTR_7g088790 40.710 479 273 6 4 474 1 476 3.10e-122 370
MS.gene052754.t1 MTR_5g012270 42.952 454 247 6 30 473 25 476 8.66e-122 368
MS.gene052754.t1 MTR_1g041815 43.844 463 248 5 17 473 10 466 1.33e-121 368
MS.gene052754.t1 MTR_3g005010 42.457 464 233 7 16 474 12 446 4.16e-121 365
MS.gene052754.t1 MTR_7g098220 44.084 431 228 3 51 474 3 427 4.39e-120 363
MS.gene052754.t1 MTR_4g019580 40.042 472 276 5 4 473 6 472 2.71e-119 362
MS.gene052754.t1 MTR_7g006540 42.105 475 253 10 17 474 44 513 2.09e-118 361
MS.gene052754.t1 MTR_4g033375 40.217 460 249 6 20 474 18 456 2.95e-117 353
MS.gene052754.t1 MTR_5g014150 41.075 465 263 5 18 473 28 490 3.15e-117 357
MS.gene052754.t1 MTR_1g042310 40.299 469 269 5 12 474 4 467 3.56e-117 357
MS.gene052754.t1 MTR_1g116930 39.565 460 268 5 17 473 7 459 8.09e-117 355
MS.gene052754.t1 MTR_7g088820 38.641 471 279 5 11 474 5 472 8.26e-117 355
MS.gene052754.t1 MTR_3g069500 41.401 471 261 7 7 474 6 464 1.21e-116 355
MS.gene052754.t1 MTR_4g098800 40.710 479 270 6 3 473 5 477 2.02e-115 352
MS.gene052754.t1 MTR_4g098800 40.756 476 268 6 6 473 73 542 6.68e-115 353
MS.gene052754.t1 MTR_7g065080 39.413 477 269 9 1 473 1 461 1.01e-114 350
MS.gene052754.t1 MTR_5g038060 41.703 458 252 6 28 473 25 479 3.38e-114 349
MS.gene052754.t1 MTR_4g033395 40.000 460 250 7 20 474 18 456 9.51e-114 345
MS.gene052754.t1 MTR_4g033315 40.343 466 253 6 13 474 10 454 5.72e-112 342
MS.gene052754.t1 MTR_4g033310 40.343 466 253 6 13 474 10 454 5.72e-112 342
MS.gene052754.t1 MTR_7g005930 39.168 457 259 6 25 473 26 471 8.29e-111 340
MS.gene052754.t1 MTR_1g041885 42.155 427 231 5 53 474 1 416 1.06e-110 338
MS.gene052754.t1 MTR_3g014790 40.899 467 248 5 19 474 2 451 1.83e-110 338
MS.gene052754.t1 MTR_3g069420 39.914 466 271 7 11 473 13 472 4.40e-110 338
MS.gene052754.t1 MTR_4g033325 40.257 467 250 7 13 474 10 452 1.25e-109 336
MS.gene052754.t1 MTR_0294s0020 40.129 466 252 7 13 474 10 452 2.07e-109 335
MS.gene052754.t1 MTR_8g038880 41.630 454 233 5 34 474 20 454 5.48e-109 335
MS.gene052754.t1 MTR_4g033330 40.167 478 258 7 1 474 1 454 1.57e-107 330
MS.gene052754.t1 MTR_3g069440 40.700 457 264 5 20 473 19 471 2.69e-107 331
MS.gene052754.t1 MTR_7g013270 39.434 459 257 7 25 473 81 528 8.45e-107 331
MS.gene052754.t1 MTR_7g088820 40.148 406 236 4 76 474 5 410 8.01e-105 323
MS.gene052754.t1 MTR_1g042310 40.000 430 247 5 51 474 6 430 2.92e-104 322
MS.gene052754.t1 MTR_4g114340 42.887 471 249 9 12 473 2 461 2.96e-104 323
MS.gene052754.t1 MTR_8g469480 39.421 449 257 6 38 474 42 487 2.23e-103 321
MS.gene052754.t1 MTR_1g042310 39.720 428 247 5 53 474 1 423 1.80e-102 317
MS.gene052754.t1 MTR_7g111260 37.838 444 261 6 32 474 17 446 3.58e-102 317
MS.gene052754.t1 MTR_8g087780 38.593 469 274 6 20 474 7 475 8.30e-102 317
MS.gene052754.t1 MTR_2g017750 37.067 491 282 10 3 474 8 490 1.61e-98 308
MS.gene052754.t1 MTR_5g085850 40.340 471 258 8 11 473 7 462 9.16e-98 306
MS.gene052754.t1 MTR_4g107580 37.862 449 265 7 38 473 28 475 2.72e-97 305
MS.gene052754.t1 MTR_3g100980 37.694 451 264 6 32 473 14 456 3.89e-97 304
MS.gene052754.t1 MTR_4g107510 37.086 453 262 8 38 473 40 486 4.60e-97 305
MS.gene052754.t1 MTR_7g098200 42.265 362 196 4 120 474 1 356 1.46e-96 300
MS.gene052754.t1 MTR_1g085090 37.303 445 262 8 32 474 17 446 1.69e-96 303
MS.gene052754.t1 MTR_8g087810 38.298 470 275 6 20 474 7 476 1.94e-96 303
MS.gene052754.t1 MTR_7g065080 40.104 384 217 5 92 473 2 374 2.60e-95 297
MS.gene052754.t1 MTR_4g107530 36.307 482 278 11 11 474 14 484 1.06e-93 296
MS.gene052754.t1 MTR_4g107670 36.439 483 274 9 12 473 2 472 1.90e-93 295
MS.gene052754.t1 MTR_2g005130 35.084 476 283 8 3 474 11 464 2.69e-92 292
MS.gene052754.t1 MTR_8g469310 38.528 462 273 7 21 473 13 472 3.02e-92 291
MS.gene052754.t1 MTR_8g469470 34.702 487 297 7 7 480 12 490 2.75e-91 289
MS.gene052754.t1 MTR_3g100980 35.491 479 264 7 32 473 14 484 4.18e-91 289
MS.gene052754.t1 MTR_7g088820 39.503 362 212 4 120 474 1 362 3.02e-89 281
MS.gene052754.t1 MTR_5g038190 35.808 458 278 7 27 473 18 470 9.18e-88 280
MS.gene052754.t1 MTR_7g064780 34.388 474 299 6 11 475 3 473 1.47e-87 280
MS.gene052754.t1 MTR_5g038380 35.991 464 285 7 20 473 14 475 3.97e-87 278
MS.gene052754.t1 MTR_4g123537 36.134 476 283 9 2 474 13 470 5.45e-87 279
MS.gene052754.t1 MTR_4g107610 36.166 459 278 8 25 474 22 474 1.10e-86 278
MS.gene052754.t1 MTR_7g064800 36.638 464 266 12 30 475 22 475 4.01e-85 273
MS.gene052754.t1 MTR_1g009200 36.184 456 266 9 32 473 25 469 6.41e-85 273
MS.gene052754.t1 MTR_4g033325 39.612 361 189 7 119 474 18 354 5.11e-82 261
MS.gene052754.t1 MTR_4g033385 39.335 361 193 7 119 474 16 355 4.59e-80 256
MS.gene052754.t1 MTR_1g026740 33.775 453 284 9 30 473 13 458 3.05e-79 257
MS.gene052754.t1 MTR_4g033325 39.881 336 180 5 144 474 10 328 5.21e-79 253
MS.gene052754.t1 MTR_8g103233 35.307 456 271 11 32 474 15 459 5.85e-77 252
MS.gene052754.t1 MTR_2g101650 32.985 479 294 13 12 474 2 469 8.17e-75 246
MS.gene052754.t1 MTR_1g026750 33.333 486 284 13 1 473 1 459 2.54e-74 244
MS.gene052754.t1 MTR_2g101640 32.599 454 289 10 32 474 21 468 1.87e-73 244
MS.gene052754.t1 MTR_6g008690 36.123 454 244 9 40 474 13 439 2.22e-73 241
MS.gene052754.t1 MTR_2g101590 33.991 456 280 11 32 474 24 471 3.69e-73 243
MS.gene052754.t1 MTR_2g101610 33.913 460 283 11 28 474 21 472 6.76e-73 243
MS.gene052754.t1 MTR_1g057460 34.298 449 269 11 32 474 42 470 1.36e-70 235
MS.gene052754.t1 MTR_1g026770 32.287 446 287 9 36 473 17 455 3.18e-69 231
MS.gene052754.t1 MTR_4g011280 32.000 450 287 10 36 474 34 475 9.74e-67 225
MS.gene052754.t1 MTR_1g057460 34.368 419 249 11 62 474 1 399 2.33e-66 222
MS.gene052754.t1 MTR_4g024930 34.244 476 273 14 11 474 11 458 1.25e-65 221
MS.gene052754.t1 MTR_2g101560 30.753 465 299 9 24 474 17 472 6.15e-62 213
MS.gene052754.t1 MTR_0236s0050 33.732 418 253 10 62 474 1 399 1.11e-61 209
MS.gene052754.t1 MTR_7g098210 33.953 430 191 11 53 474 1 345 1.37e-61 207
MS.gene052754.t1 MTR_2g093160 30.342 468 300 8 20 474 2 456 8.60e-61 208
MS.gene052754.t1 MTR_2g093180 30.426 470 298 9 20 474 2 457 5.15e-58 201
MS.gene052754.t1 MTR_2g093640 28.842 475 298 10 20 474 2 456 9.93e-55 192
MS.gene052754.t1 MTR_7g051880 28.947 456 290 8 19 474 10 431 4.02e-53 187
MS.gene052754.t1 MTR_7g051830 28.947 456 290 8 19 474 10 431 1.46e-52 186
MS.gene052754.t1 MTR_7g052600 28.728 456 291 8 19 474 10 431 4.84e-52 184
MS.gene052754.t1 MTR_1g009170 32.523 329 167 8 153 473 5 286 4.97e-44 159
MS.gene052754.t1 MTR_4g033310 45.455 187 95 2 13 199 10 189 4.99e-43 159
MS.gene052754.t1 MTR_4g033310 37.949 195 113 2 282 474 202 390 4.92e-39 148
MS.gene052754.t1 MTR_3g065350 38.519 135 77 2 340 474 1 129 4.91e-22 93.2
MS.gene052754.t1 MTR_7g064160 34.375 96 63 0 11 106 6 101 1.39e-15 73.6
MS.gene052754.t1 MTR_8g091265 35.238 105 64 2 304 404 5 109 5.02e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052754.t1 AT3G54140 75.647 464 111 2 12 473 2 465 0.0 742
MS.gene052754.t1 AT3G54140 75.647 464 111 2 12 473 2 465 0.0 742
MS.gene052754.t1 AT5G01180 72.258 465 128 1 10 473 1 465 0.0 716
MS.gene052754.t1 AT5G01180 72.258 465 128 1 10 473 1 465 0.0 716
MS.gene052754.t1 AT5G01180 72.258 465 128 1 10 473 1 465 0.0 716
MS.gene052754.t1 AT2G02040 59.615 468 180 6 12 473 19 483 0.0 567
MS.gene052754.t1 AT1G62200 56.133 481 190 7 2 474 23 490 0.0 541
MS.gene052754.t1 AT1G62200 53.137 510 189 9 2 474 37 533 0.0 526
MS.gene052754.t1 AT1G62200 53.137 510 189 9 2 474 23 519 0.0 526
MS.gene052754.t1 AT2G02020 52.463 467 182 6 10 473 18 447 2.24e-170 491
MS.gene052754.t1 AT2G02020 47.752 467 183 8 10 473 18 426 1.09e-144 425
MS.gene052754.t1 AT1G68570 47.020 453 232 5 29 474 19 470 5.81e-128 384
MS.gene052754.t1 AT2G40460 42.060 466 258 5 12 474 2 458 1.61e-127 383
MS.gene052754.t1 AT5G46040 41.949 472 261 4 10 474 3 468 3.42e-126 379
MS.gene052754.t1 AT1G68570 47.477 436 222 4 46 474 5 440 5.04e-126 379
MS.gene052754.t1 AT5G46050 41.102 472 265 4 10 474 3 468 5.90e-124 374
MS.gene052754.t1 AT2G37900 42.171 479 260 8 1 473 1 468 2.75e-120 364
MS.gene052754.t1 AT1G12110 42.609 460 253 7 24 474 17 474 2.61e-118 359
MS.gene052754.t1 AT5G13400 41.026 468 255 8 23 474 52 514 6.18e-116 354
MS.gene052754.t1 AT3G54450 40.265 452 248 6 31 474 9 446 2.90e-115 350
MS.gene052754.t1 AT3G53960 41.394 459 260 8 20 474 23 476 5.57e-113 346
MS.gene052754.t1 AT4G21680 41.453 468 258 8 18 473 12 475 2.36e-112 344
MS.gene052754.t1 AT2G26690 43.617 470 246 9 12 473 2 460 5.54e-112 343
MS.gene052754.t1 AT1G69870 41.410 454 250 10 32 474 49 497 6.27e-112 344
MS.gene052754.t1 AT5G19640 39.501 481 265 7 2 473 22 485 2.78e-111 342
MS.gene052754.t1 AT1G32450 39.666 479 274 7 6 473 12 486 2.88e-110 339
MS.gene052754.t1 AT2G37900 44.110 399 210 7 77 473 2 389 3.27e-105 323
MS.gene052754.t1 AT1G72125 40.842 475 253 10 7 474 6 459 1.34e-104 323
MS.gene052754.t1 AT2G26690 44.266 436 224 9 46 473 3 427 4.21e-104 321
MS.gene052754.t1 AT1G59740 38.166 469 277 5 11 474 18 478 4.69e-104 323
MS.gene052754.t1 AT1G22540 39.619 472 260 7 8 474 4 455 4.89e-104 322
MS.gene052754.t1 AT1G72120 38.793 464 257 6 17 474 13 455 1.45e-100 313
MS.gene052754.t1 AT2G26690 44.235 425 218 9 57 473 2 415 1.47e-100 312
MS.gene052754.t1 AT1G72140 36.117 479 264 6 9 474 6 455 1.46e-99 310
MS.gene052754.t1 AT1G22570 38.382 482 264 8 10 475 3 467 1.84e-99 310
MS.gene052754.t1 AT1G72130 37.634 465 240 10 18 474 14 436 2.28e-99 309
MS.gene052754.t1 AT3G21670 41.614 471 242 10 19 473 11 464 2.19e-98 308
MS.gene052754.t1 AT1G69850 38.351 485 267 8 13 473 4 480 6.78e-98 306
MS.gene052754.t1 AT1G22570 38.964 444 241 7 45 475 4 430 1.46e-93 293
MS.gene052754.t1 AT1G22550 37.449 486 267 8 10 479 3 467 2.85e-93 294
MS.gene052754.t1 AT1G27040 38.429 471 253 10 20 473 13 463 1.48e-92 292
MS.gene052754.t1 AT1G27040 38.429 471 253 10 20 473 9 459 1.48e-92 292
MS.gene052754.t1 AT1G18880 36.504 452 267 11 38 474 23 469 1.27e-91 290
MS.gene052754.t1 AT1G27080 36.344 465 261 10 26 474 6 451 2.34e-91 289
MS.gene052754.t1 AT1G33440 35.654 474 284 7 7 474 11 469 3.31e-89 284
MS.gene052754.t1 AT5G46050 40.884 362 201 4 120 474 1 356 1.20e-88 279
MS.gene052754.t1 AT1G27040 38.667 450 239 10 41 473 2 431 1.76e-88 281
MS.gene052754.t1 AT1G52190 35.870 460 272 10 32 474 19 472 2.65e-88 282
MS.gene052754.t1 AT5G28470 34.590 451 281 6 32 474 15 459 9.19e-87 277
MS.gene052754.t1 AT5G14940 34.707 461 261 10 32 474 5 443 3.04e-84 270
MS.gene052754.t1 AT5G28470 34.368 451 280 7 32 474 15 457 8.86e-84 269
MS.gene052754.t1 AT3G16180 35.435 460 272 9 32 474 21 472 1.00e-83 270
MS.gene052754.t1 AT5G62680 35.394 469 281 11 24 474 31 495 1.28e-82 268
MS.gene052754.t1 AT5G62730 34.369 483 270 8 10 473 32 486 5.41e-82 265
MS.gene052754.t1 AT3G47960 34.409 465 289 9 23 474 50 511 5.22e-80 261
MS.gene052754.t1 AT5G62730 35.320 453 246 8 40 473 3 427 6.75e-79 255
MS.gene052754.t1 AT1G27080 35.681 426 239 10 65 474 3 409 1.70e-78 254
MS.gene052754.t1 AT1G69860 33.983 462 285 9 19 473 4 452 2.74e-75 247
MS.gene052754.t1 AT3G45650 31.681 464 293 8 18 474 6 452 6.15e-75 246
MS.gene052754.t1 AT3G45650 31.681 464 293 8 18 474 6 452 1.24e-74 246
MS.gene052754.t1 AT3G45660 32.867 429 264 8 53 474 40 451 1.38e-72 239
MS.gene052754.t1 AT3G45660 32.867 429 264 8 53 474 40 451 1.38e-72 239
MS.gene052754.t1 AT3G01350 32.906 468 272 11 31 474 4 453 1.62e-72 239
MS.gene052754.t1 AT3G45660 32.867 429 264 8 53 474 61 472 2.19e-72 239
MS.gene052754.t1 AT1G72130 37.202 336 172 7 147 474 14 318 2.08e-70 230
MS.gene052754.t1 AT1G72130 37.202 336 172 7 147 474 14 318 2.08e-70 230
MS.gene052754.t1 AT3G45720 31.004 458 292 10 24 474 9 449 3.97e-67 225
MS.gene052754.t1 AT3G45680 30.804 448 287 10 34 474 20 451 1.08e-65 221
MS.gene052754.t1 AT3G25260 31.441 458 276 7 19 474 6 427 2.01e-65 219
MS.gene052754.t1 AT3G45710 29.825 456 298 9 26 474 13 453 1.63e-62 213
MS.gene052754.t1 AT3G45700 30.435 460 281 13 27 474 9 441 5.67e-62 211
MS.gene052754.t1 AT3G25280 30.937 459 277 7 19 474 6 427 3.40e-61 209
MS.gene052754.t1 AT3G45690 28.761 452 297 9 29 476 13 443 1.46e-60 207
MS.gene052754.t1 AT5G11570 38.618 246 129 8 43 279 22 254 3.67e-43 159
MS.gene052754.t1 AT5G11570 30.968 155 101 2 321 474 237 386 1.91e-15 79.0
MS.gene052754.t1 AT2G38100 27.381 420 273 8 62 475 2 395 5.43e-38 145
MS.gene052754.t1 AT2G38100 27.381 420 273 8 62 475 18 411 1.36e-37 144
MS.gene052754.t1 AT2G38100 27.381 420 273 8 62 475 24 417 1.53e-37 144

Find 115 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTGTGTGTTCAAGTTTC+CGG 0.188591 4.4:-85674344 None:intergenic
GTCGGATGGGGATGGGGTTT+TGG 0.221483 4.4:+85674117 MS.gene052754:CDS
AAACCATGTGTTTCATCTTT+TGG 0.233525 4.4:+85673964 MS.gene052754:CDS
ACACTCTAAGTGTCATCTTC+TGG 0.233776 4.4:+85674791 MS.gene052754:CDS
TCAAGTAGAGTGTTGTAAAA+AGG 0.241063 4.4:-85673918 None:intergenic
ACTCATTGCTTCGTCGGTTT+TGG 0.280390 4.4:+85674077 MS.gene052754:CDS
TTCCCCTGATTTCAGGTGTT+TGG 0.286073 4.4:+85674522 MS.gene052754:intron
AAGTTATTGTTGCAGCATTT+AGG 0.328500 4.4:+85674244 MS.gene052754:CDS
CATGTATGCATCAGCTAAAA+AGG 0.335601 4.4:-85673588 None:intergenic
CACTCTAAGTGTCATCTTCT+GGG 0.360635 4.4:+85674792 MS.gene052754:CDS
CGTTCGTTTACTCCCCGTTT+GGG 0.363000 4.4:+85674642 MS.gene052754:CDS
AAGCCTACATGTGATACTAA+TGG 0.372786 4.4:+85673865 MS.gene052754:CDS
CCATCCCTATGTCAATCTTC+TGG 0.378069 4.4:+85675001 MS.gene052754:CDS
TCGTTCGTTTACTCCCCGTT+TGG 0.403486 4.4:+85674641 MS.gene052754:CDS
TGATTTCAGGTGTTTGGATA+AGG 0.407783 4.4:+85674528 MS.gene052754:intron
TACTTGATTGCGCTTGGAAC+CGG 0.414135 4.4:+85673934 MS.gene052754:CDS
GTCATCTTGATTACTTCTAC+TGG 0.423570 4.4:+85675253 MS.gene052754:CDS
ATCATTTCCATGATCGTGGC+GGG 0.429802 4.4:+85674919 MS.gene052754:CDS
GTTGCAGCATTTAGGAAACT+TGG 0.431571 4.4:+85674252 MS.gene052754:CDS
CTTGAAGACCGATACGGGCT+TGG 0.437058 4.4:+85673497 MS.gene052754:CDS
AGCCTACATGTGATACTAAT+GGG 0.437537 4.4:+85673866 MS.gene052754:CDS
CGCCACGATCATGGAAATGA+TGG 0.441529 4.4:-85674917 None:intergenic
ACTTGACAAATCCTTGTAAG+AGG 0.442092 4.4:-85674225 None:intergenic
CAGCTTCATAGAATCGGAAT+TGG 0.442210 4.4:+85674883 MS.gene052754:CDS
TGCAACGGTATACAGTCAAA+TGG 0.443310 4.4:+85674681 MS.gene052754:CDS
AAAGCATGCCGCTTTATTCT+CGG 0.445366 4.4:+85673147 MS.gene052754:CDS
TAAAGAAGAGACTTATCATC+TGG 0.447791 4.4:-85674285 None:intergenic
GGCTATCAGTGATGCCCAAA+CGG 0.448610 4.4:-85674656 None:intergenic
ACACTCTACTTGATTGCGCT+TGG 0.452235 4.4:+85673928 MS.gene052754:CDS
ATGAATGTCGGATGGGGATG+GGG 0.453174 4.4:+85674111 MS.gene052754:CDS
TAGAATCCCCGCCACGATCA+TGG 0.456374 4.4:-85674926 None:intergenic
GTGCAGAGCCTCCATGGATT+CGG 0.457325 4.4:-85674589 None:intergenic
GCTATCAGTGATGCCCAAAC+GGG 0.459760 4.4:-85674655 None:intergenic
TGACACTTAGAGTGTCGAAA+AGG 0.462376 4.4:-85674783 None:intergenic
GATGAATGTCGGATGGGGAT+GGG 0.465810 4.4:+85674110 MS.gene052754:CDS
GCTGTGTGAATCCGTGTTCA+TGG 0.471308 4.4:-85674864 None:intergenic
CTATGGCATGAGTACAAACT+TGG 0.471382 4.4:+85673466 MS.gene052754:CDS
ATGCATACATGGGAAGATAC+TGG 0.472511 4.4:+85673600 MS.gene052754:CDS
AGATGAATGTCGGATGGGGA+TGG 0.477086 4.4:+85674109 MS.gene052754:CDS
TGTGAAAGACTGGCTTACTA+TGG 0.485888 4.4:+85673449 MS.gene052754:CDS
TTCACACAGCTTCATAGAAT+CGG 0.489704 4.4:+85674877 MS.gene052754:CDS
TGTTACATCACACCATTGCT+TGG 0.490210 4.4:+85673563 MS.gene052754:CDS
CGGTGCACTCATTGCTTCGT+CGG 0.490292 4.4:+85674071 MS.gene052754:CDS
GATGGTTTATATACAGAAGA+TGG 0.492031 4.4:+85673072 MS.gene052754:CDS
GGAACCATGTTTGTCTTACA+AGG 0.496339 4.4:+85674703 MS.gene052754:CDS
ACGATGAGACGGTCGTAGAC+CGG 0.502734 4.4:-85674817 None:intergenic
CTGCAAATACTGTCACAACT+TGG 0.505268 4.4:+85673531 MS.gene052754:CDS
CGATGAGACGGTCGTAGACC+GGG 0.505276 4.4:-85674816 None:intergenic
AAGTGTCATCTTCTGGGCCC+CGG 0.506775 4.4:+85674798 MS.gene052754:CDS
AATGGGTGCCATCCGACGTC+AGG 0.511790 4.4:+85673883 MS.gene052754:CDS
ATGTAGAATCTAACATCAAA+GGG 0.513359 4.4:+85674319 MS.gene052754:CDS
GACACTTAGAGTGTCGAAAA+GGG 0.515286 4.4:-85674782 None:intergenic
GAAGATGTCAATATAAAAGC+TGG 0.515786 4.4:+85673036 MS.gene052754:CDS
CGGTGAACTTTCTTGCGAAA+GGG 0.518248 4.4:-85674840 None:intergenic
AATACTGTCACAACTTGGTC+GGG 0.519066 4.4:+85673536 MS.gene052754:CDS
TAAAAGATTTACCGAATCCA+TGG 0.520585 4.4:+85674578 MS.gene052754:CDS
AACTATCTTGAAGACCGATA+CGG 0.524878 4.4:+85673491 MS.gene052754:CDS
GTTACTGTGCAGAGCCTCCA+TGG 0.528525 4.4:-85674595 None:intergenic
CCGGTGAACTTTCTTGCGAA+AGG 0.533554 4.4:-85674841 None:intergenic
GATGTAGAATCTAACATCAA+AGG 0.533602 4.4:+85674318 MS.gene052754:CDS
AAATACTGTCACAACTTGGT+CGG 0.534850 4.4:+85673535 MS.gene052754:CDS
CTGCATTTCCAAGCCCGTAT+CGG 0.535168 4.4:-85673505 None:intergenic
CTGGTGGAAGTCCTCTTACA+AGG 0.537556 4.4:+85674214 MS.gene052754:CDS
GCAACGGTATACAGTCAAAT+GGG 0.538160 4.4:+85674682 MS.gene052754:CDS
TGGAGTACCTGGAGTTGCAA+TGG 0.538447 4.4:+85674137 MS.gene052754:CDS
AAAAGGGAGAGGGAGGCTGA+TGG 0.541173 4.4:-85674766 None:intergenic
AAATGAATGTTGTGAAAGAC+TGG 0.544472 4.4:+85673439 MS.gene052754:CDS
TTGCAGCATTTAGGAAACTT+GGG 0.546918 4.4:+85674253 MS.gene052754:CDS
TTGCGAAAGGGACGATGAGA+CGG 0.547490 4.4:-85674828 None:intergenic
CAGTTTGTCCTGACGTCGGA+TGG 0.548403 4.4:-85673891 None:intergenic
ACTATCTTGAAGACCGATAC+GGG 0.549793 4.4:+85673492 MS.gene052754:CDS
AATCTAACATCAAAGGGAGC+CGG 0.551381 4.4:+85674325 MS.gene052754:CDS
TGTGAATCCGTGTTCATGGC+CGG 0.553955 4.4:-85674860 None:intergenic
AACACATGGTTTGATACCGC+CGG 0.557643 4.4:-85673953 None:intergenic
ACTTGCCAGAAGATTGACAT+AGG 0.572578 4.4:-85675006 None:intergenic
GGATACAGATGAATGTCGGA+TGG 0.573725 4.4:+85674103 MS.gene052754:CDS
GCGATAACCATTGCAACTCC+AGG 0.576615 4.4:-85674144 None:intergenic
TGGAAATGATGGAGATGACT+AGG 0.578345 4.4:-85674906 None:intergenic
TTAGAGTGTCGAAAAGGGAG+AGG 0.583008 4.4:-85674777 None:intergenic
CTCCATCATTTCCATGATCG+TGG 0.586521 4.4:+85674915 MS.gene052754:CDS
GATACAGATGAATGTCGGAT+GGG 0.590289 4.4:+85674104 MS.gene052754:CDS
TCATTTCCATGATCGTGGCG+GGG 0.592240 4.4:+85674920 MS.gene052754:CDS
TTGATTGCGCTTGGAACCGG+CGG 0.592364 4.4:+85673937 MS.gene052754:CDS
CAGTAGAGACAGAATCCGAC+CGG 0.595827 4.4:+85674554 MS.gene052754:CDS
TGGTATAGACTTCAAATACC+TGG 0.596885 4.4:+85674195 MS.gene052754:CDS
TAGAGTGTCGAAAAGGGAGA+GGG 0.597883 4.4:-85674776 None:intergenic
CTTTAGTCCAGGTGCAATAG+CGG 0.599030 4.4:-85673845 None:intergenic
TTGACTGTATACCGTTGCAA+AGG 0.610335 4.4:-85674677 None:intergenic
AGTGTCGAAAAGGGAGAGGG+AGG 0.610825 4.4:-85674773 None:intergenic
AAGTTCACCGGCCATGAACA+CGG 0.612897 4.4:+85674853 MS.gene052754:CDS
CTTGTAAGAGGACTTCCACC+AGG 0.614606 4.4:-85674213 None:intergenic
GTTTGGATACAGATGAATGT+CGG 0.623210 4.4:+85674099 MS.gene052754:CDS
GCTGGTGTGAATGAAGAAGA+TGG 0.624270 4.4:+85673054 MS.gene052754:CDS
TCACATGTAGGCTTTAGTCC+AGG 0.627179 4.4:-85673856 None:intergenic
CATCATTTCCATGATCGTGG+CGG 0.628187 4.4:+85674918 MS.gene052754:CDS
ATTTAATACCGAGAATAAAG+CGG 0.636396 4.4:-85673155 None:intergenic
GTACCGCAGTATTTCCTCGT+AGG 0.638440 4.4:+85675027 MS.gene052754:CDS
CCTTTCGCAAGAAAGTTCAC+CGG 0.638836 4.4:+85674841 MS.gene052754:CDS
CCAGAAGATTGACATAGGGA+TGG 0.641041 4.4:-85675001 None:intergenic
CTTGCCAGAAGATTGACATA+GGG 0.645695 4.4:-85675005 None:intergenic
ATCACTGATAGCCTTTGCAA+CGG 0.646848 4.4:+85674666 MS.gene052754:CDS
ATACAGATGAATGTCGGATG+GGG 0.656151 4.4:+85674105 MS.gene052754:CDS
AACACAATGGATCAACACAT+TGG 0.661215 4.4:+85674727 MS.gene052754:CDS
ATGAAAACGATGAGACTGAG+AGG 0.663289 4.4:+85674001 MS.gene052754:CDS
TGTCTTACAAGGCAACACAA+TGG 0.666103 4.4:+85674714 MS.gene052754:CDS
CACCCATTAGTATCACATGT+AGG 0.666111 4.4:-85673868 None:intergenic
TATAGACTTCAAATACCTGG+TGG 0.669427 4.4:+85674198 MS.gene052754:CDS
AAACTTCGGCGGCACCTACG+AGG 0.673774 4.4:-85675041 None:intergenic
GCTGCAGTTTGTCCTGACGT+CGG 0.674367 4.4:-85673895 None:intergenic
GATGAGACGGTCGTAGACCG+GGG 0.681272 4.4:-85674815 None:intergenic
CTATCAGTGATGCCCAAACG+GGG 0.694144 4.4:-85674654 None:intergenic
AAGATTTACCGAATCCATGG+AGG 0.715910 4.4:+85674581 MS.gene052754:CDS
GTTGCCTTGTAAGACAAACA+TGG 0.717073 4.4:-85674707 None:intergenic
GCACCAAAAGATGAAACACA+TGG 0.722888 4.4:-85673967 None:intergenic
GCACCTACGAGGAAATACTG+CGG 0.845378 4.4:-85675030 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAAAGATAAATATAAAAA+TGG - chr4.4:85674391-85674410 None:intergenic 10.0%
!!! TTTTACTTCTCAATTAATAT+CGG + chr4.4:85674051-85674070 MS.gene052754:CDS 15.0%
!!! ATGAAGTTTAGAATAAGAAA+TGG - chr4.4:85674433-85674452 None:intergenic 20.0%
!!! TTGTTGAAATGTTTTGATAA+AGG + chr4.4:85673687-85673706 MS.gene052754:intron 20.0%
! ACAAAAGTTACTACAAGAAA+TGG + chr4.4:85675195-85675214 MS.gene052754:intron 25.0%
! ACCGATGAAGAAAAATATAA+TGG - chr4.4:85674169-85674188 None:intergenic 25.0%
! AGTTCATATTTAAATGTCTG+AGG + chr4.4:85673737-85673756 MS.gene052754:intron 25.0%
! ATGTAGAATCTAACATCAAA+GGG + chr4.4:85674319-85674338 MS.gene052754:CDS 25.0%
! ATTCCAGATAATATGAATAG+GGG + chr4.4:85675231-85675250 MS.gene052754:intron 25.0%
! ATTTAATACCGAGAATAAAG+CGG - chr4.4:85673158-85673177 None:intergenic 25.0%
! CAACTTGTGTTTAAATACTA+CGG - chr4.4:85673237-85673256 None:intergenic 25.0%
! GATTCCAGATAATATGAATA+GGG + chr4.4:85675230-85675249 MS.gene052754:intron 25.0%
! TTCATAATCAAAATGTCTGA+GGG + chr4.4:85673760-85673779 MS.gene052754:intron 25.0%
!! GCCATTATATTTTTCTTCAT+CGG + chr4.4:85674165-85674184 MS.gene052754:CDS 25.0%
!!! AGAAAAGCTCTTTTTTCAAT+TGG + chr4.4:85674028-85674047 MS.gene052754:CDS 25.0%
AAATGAATGTTGTGAAAGAC+TGG + chr4.4:85673439-85673458 MS.gene052754:CDS 30.0%
AAGTTATTGTTGCAGCATTT+AGG + chr4.4:85674244-85674263 MS.gene052754:CDS 30.0%
ACAAGAAAAAGACAGGAAAT+TGG + chr4.4:85673124-85673143 MS.gene052754:CDS 30.0%
ACCGATATTAGTGAAAACTT+CGG - chr4.4:85675058-85675077 None:intergenic 30.0%
GAAGATGTCAATATAAAAGC+TGG + chr4.4:85673036-85673055 MS.gene052754:CDS 30.0%
GATGGTTTATATACAGAAGA+TGG + chr4.4:85673072-85673091 MS.gene052754:CDS 30.0%
GATGTAGAATCTAACATCAA+AGG + chr4.4:85674318-85674337 MS.gene052754:CDS 30.0%
GGATTCCAGATAATATGAAT+AGG + chr4.4:85675229-85675248 MS.gene052754:intron 30.0%
GTTCATAATCAAAATGTCTG+AGG + chr4.4:85673759-85673778 MS.gene052754:intron 30.0%
TAAAAGATTTACCGAATCCA+TGG + chr4.4:85674578-85674597 MS.gene052754:CDS 30.0%
TAAAGAAGAGACTTATCATC+TGG - chr4.4:85674288-85674307 None:intergenic 30.0%
TCACTTACAACTAATGCATT+GGG + chr4.4:85675138-85675157 MS.gene052754:intron 30.0%
TCATATCCGATATATATTCC+TGG + chr4.4:85673210-85673229 MS.gene052754:intron 30.0%
TTCACTTACAACTAATGCAT+TGG + chr4.4:85675137-85675156 MS.gene052754:intron 30.0%
! AAACCATGTGTTTCATCTTT+TGG + chr4.4:85673964-85673983 MS.gene052754:CDS 30.0%
! ATCTTTAAGAGACTACTCAT+TGG + chr4.4:85674402-85674421 MS.gene052754:intron 30.0%
! ATTTCCTGTCTTTTTCTTGT+TGG - chr4.4:85673124-85673143 None:intergenic 30.0%
! GCTGATGGAATTTTGAATTT+AGG - chr4.4:85674754-85674773 None:intergenic 30.0%
! TCTCATCGTTTTCATCAAAT+TGG - chr4.4:85673996-85674015 None:intergenic 30.0%
!! CAAGTAGAGTGTTGTAAAAA+GGG - chr4.4:85673920-85673939 None:intergenic 30.0%
!! GATTCGGTAAATCTTTTATC+CGG - chr4.4:85674576-85674595 None:intergenic 30.0%
!! TCAAGTAGAGTGTTGTAAAA+AGG - chr4.4:85673921-85673940 None:intergenic 30.0%
!!! GATCATAGTAGTTGTTTTTG+CGG - chr4.4:85674975-85674994 None:intergenic 30.0%
!!! TTTTTAGCTGATGCATACAT+GGG + chr4.4:85673590-85673609 MS.gene052754:CDS 30.0%
AAAGAAATTCCCCTGATTTC+AGG + chr4.4:85674515-85674534 MS.gene052754:intron 35.0%
AAATACTGTCACAACTTGGT+CGG + chr4.4:85673535-85673554 MS.gene052754:CDS 35.0%
AACACAATGGATCAACACAT+TGG + chr4.4:85674727-85674746 MS.gene052754:CDS 35.0%
AACTATCTTGAAGACCGATA+CGG + chr4.4:85673491-85673510 MS.gene052754:CDS 35.0%
AAGCCTACATGTGATACTAA+TGG + chr4.4:85673865-85673884 MS.gene052754:CDS 35.0%
AATTGTTAGTACAACAACGC+TGG - chr4.4:85673380-85673399 None:intergenic 35.0%
ACTACAAGAAATGGAAGTCT+TGG + chr4.4:85675204-85675223 MS.gene052754:intron 35.0%
ACTTGACAAATCCTTGTAAG+AGG - chr4.4:85674228-85674247 None:intergenic 35.0%
AGCCTACATGTGATACTAAT+GGG + chr4.4:85673866-85673885 MS.gene052754:CDS 35.0%
ATCACACGAAAAATTGTTGC+AGG + chr4.4:85673416-85673435 MS.gene052754:intron 35.0%
CATAAACTTCTCATAGCATC+AGG - chr4.4:85675108-85675127 None:intergenic 35.0%
CATGTATGCATCAGCTAAAA+AGG - chr4.4:85673591-85673610 None:intergenic 35.0%
GATATTAGTGAAAACTTCGG+CGG - chr4.4:85675055-85675074 None:intergenic 35.0%
GTCATCTTGATTACTTCTAC+TGG + chr4.4:85675253-85675272 MS.gene052754:CDS 35.0%
GTTTGGATACAGATGAATGT+CGG + chr4.4:85674099-85674118 MS.gene052754:CDS 35.0%
TACGGTCCAGGAATATATAT+CGG - chr4.4:85673219-85673238 None:intergenic 35.0%
TATAGACTTCAAATACCTGG+TGG + chr4.4:85674198-85674217 MS.gene052754:CDS 35.0%
TATCCAAACACCTGAAATCA+GGG - chr4.4:85674528-85674547 None:intergenic 35.0%
TGACCCCTATTCATATTATC+TGG - chr4.4:85675237-85675256 None:intergenic 35.0%
TGGTATAGACTTCAAATACC+TGG + chr4.4:85674195-85674214 MS.gene052754:CDS 35.0%
TTATCCAAACACCTGAAATC+AGG - chr4.4:85674529-85674548 None:intergenic 35.0%
TTCACACAGCTTCATAGAAT+CGG + chr4.4:85674877-85674896 MS.gene052754:CDS 35.0%
TTGCAGCATTTAGGAAACTT+GGG + chr4.4:85674253-85674272 MS.gene052754:CDS 35.0%
TTTCACTAATATCGGTCAGA+TGG + chr4.4:85675062-85675081 MS.gene052754:intron 35.0%
! CATTGTTTGTGTGTTTTGCA+GGG + chr4.4:85673802-85673821 MS.gene052754:intron 35.0%
! CATTTGTGTGTTCAAGTTTC+CGG - chr4.4:85674347-85674366 None:intergenic 35.0%
! GCCGAAGTTTTCACTAATAT+CGG + chr4.4:85675054-85675073 MS.gene052754:intron 35.0%
! TCATTGTTTGTGTGTTTTGC+AGG + chr4.4:85673801-85673820 MS.gene052754:intron 35.0%
! TGATTTCAGGTGTTTGGATA+AGG + chr4.4:85674528-85674547 MS.gene052754:intron 35.0%
! TTTTCTTCATCGGTAGTAGA+TGG + chr4.4:85674175-85674194 MS.gene052754:CDS 35.0%
!!! CTTTTTAGCTGATGCATACA+TGG + chr4.4:85673589-85673608 MS.gene052754:CDS 35.0%
AAGATTTACCGAATCCATGG+AGG + chr4.4:85674581-85674600 MS.gene052754:CDS 40.0%
AATACTGTCACAACTTGGTC+GGG + chr4.4:85673536-85673555 MS.gene052754:CDS 40.0%
AATCTAACATCAAAGGGAGC+CGG + chr4.4:85674325-85674344 MS.gene052754:CDS 40.0%
ACACTCTAAGTGTCATCTTC+TGG + chr4.4:85674791-85674810 MS.gene052754:CDS 40.0%
ACTATCTTGAAGACCGATAC+GGG + chr4.4:85673492-85673511 MS.gene052754:CDS 40.0%
ACTTGCCAGAAGATTGACAT+AGG - chr4.4:85675009-85675028 None:intergenic 40.0%
ATACAGATGAATGTCGGATG+GGG + chr4.4:85674105-85674124 MS.gene052754:CDS 40.0%
ATCACTGATAGCCTTTGCAA+CGG + chr4.4:85674666-85674685 MS.gene052754:CDS 40.0%
ATCCAAACACCTGAAATCAG+GGG - chr4.4:85674527-85674546 None:intergenic 40.0%
ATGAAAACGATGAGACTGAG+AGG + chr4.4:85674001-85674020 MS.gene052754:CDS 40.0%
ATGCATACATGGGAAGATAC+TGG + chr4.4:85673600-85673619 MS.gene052754:CDS 40.0%
CAAGAAATGGAAGTCTTGGT+TGG + chr4.4:85675208-85675227 MS.gene052754:intron 40.0%
CACCCATTAGTATCACATGT+AGG - chr4.4:85673871-85673890 None:intergenic 40.0%
CACTCTAAGTGTCATCTTCT+GGG + chr4.4:85674792-85674811 MS.gene052754:CDS 40.0%
CAGCTTCATAGAATCGGAAT+TGG + chr4.4:85674883-85674902 MS.gene052754:CDS 40.0%
CTATGGCATGAGTACAAACT+TGG + chr4.4:85673466-85673485 MS.gene052754:CDS 40.0%
CTGAGGGAAAGACATGTTAA+TGG + chr4.4:85673776-85673795 MS.gene052754:intron 40.0%
CTGCAAATACTGTCACAACT+TGG + chr4.4:85673531-85673550 MS.gene052754:CDS 40.0%
CTTGCCAGAAGATTGACATA+GGG - chr4.4:85675008-85675027 None:intergenic 40.0%
GATACAGATGAATGTCGGAT+GGG + chr4.4:85674104-85674123 MS.gene052754:CDS 40.0%
GCAACGGTATACAGTCAAAT+GGG + chr4.4:85674682-85674701 MS.gene052754:CDS 40.0%
GCACCAAAAGATGAAACACA+TGG - chr4.4:85673970-85673989 None:intergenic 40.0%
GGAACCATGTTTGTCTTACA+AGG + chr4.4:85674703-85674722 MS.gene052754:CDS 40.0%
GTGTTTAAATACTACGGTCC+AGG - chr4.4:85673231-85673250 None:intergenic 40.0%
GTTGCAGCATTTAGGAAACT+TGG + chr4.4:85674252-85674271 MS.gene052754:CDS 40.0%
GTTGCCTTGTAAGACAAACA+TGG - chr4.4:85674710-85674729 None:intergenic 40.0%
TGCAACGGTATACAGTCAAA+TGG + chr4.4:85674681-85674700 MS.gene052754:CDS 40.0%
TGTCTTACAAGGCAACACAA+TGG + chr4.4:85674714-85674733 MS.gene052754:CDS 40.0%
TGTTACATCACACCATTGCT+TGG + chr4.4:85673563-85673582 MS.gene052754:CDS 40.0%
TTGACTGTATACCGTTGCAA+AGG - chr4.4:85674680-85674699 None:intergenic 40.0%
! CGGTAAATCTTTTATCCGGT+CGG - chr4.4:85674572-85674591 None:intergenic 40.0%
! GATGGAGTTCTTTTATGGTC+AGG + chr4.4:85675080-85675099 MS.gene052754:intron 40.0%
! GGTCAGATGGAGTTCTTTTA+TGG + chr4.4:85675075-85675094 MS.gene052754:intron 40.0%
! TGGAAATGATGGAGATGACT+AGG - chr4.4:85674909-85674928 None:intergenic 40.0%
! TTTGTGTGTTTTGCAGGGAA+TGG + chr4.4:85673807-85673826 MS.gene052754:intron 40.0%
!! AAAGCATGCCGCTTTATTCT+CGG + chr4.4:85673147-85673166 MS.gene052754:CDS 40.0%
!! GACACTTAGAGTGTCGAAAA+GGG - chr4.4:85674785-85674804 None:intergenic 40.0%
!! TGACACTTAGAGTGTCGAAA+AGG - chr4.4:85674786-85674805 None:intergenic 40.0%
!! TGTGAAAGACTGGCTTACTA+TGG + chr4.4:85673449-85673468 MS.gene052754:CDS 40.0%
AACACATGGTTTGATACCGC+CGG - chr4.4:85673956-85673975 None:intergenic 45.0%
ACACTCTACTTGATTGCGCT+TGG + chr4.4:85673928-85673947 MS.gene052754:CDS 45.0%
ATCATTTCCATGATCGTGGC+GGG + chr4.4:85674919-85674938 MS.gene052754:CDS 45.0%
CATCATTTCCATGATCGTGG+CGG + chr4.4:85674918-85674937 MS.gene052754:CDS 45.0%
CCAGAAGATTGACATAGGGA+TGG - chr4.4:85675004-85675023 None:intergenic 45.0%
CCATCCCTATGTCAATCTTC+TGG + chr4.4:85675001-85675020 MS.gene052754:CDS 45.0%
CCTTTCGCAAGAAAGTTCAC+CGG + chr4.4:85674841-85674860 MS.gene052754:CDS 45.0%
CGGTGAACTTTCTTGCGAAA+GGG - chr4.4:85674843-85674862 None:intergenic 45.0%
CGTGCCAACAAGAAAAAGAC+AGG + chr4.4:85673117-85673136 MS.gene052754:CDS 45.0%
CTCCATCATTTCCATGATCG+TGG + chr4.4:85674915-85674934 MS.gene052754:CDS 45.0%
CTTTAGTCCAGGTGCAATAG+CGG - chr4.4:85673848-85673867 None:intergenic 45.0%
GCTAAAAAGGCTCCAAGCAA+TGG - chr4.4:85673578-85673597 None:intergenic 45.0%
GCTGGTGTGAATGAAGAAGA+TGG + chr4.4:85673054-85673073 MS.gene052754:CDS 45.0%
GGATACAGATGAATGTCGGA+TGG + chr4.4:85674103-85674122 MS.gene052754:CDS 45.0%
TACTTGATTGCGCTTGGAAC+CGG + chr4.4:85673934-85673953 MS.gene052754:CDS 45.0%
! ACATTTTCCGCTATTGCACC+TGG + chr4.4:85673838-85673857 MS.gene052754:CDS 45.0%
! TCACATGTAGGCTTTAGTCC+AGG - chr4.4:85673859-85673878 None:intergenic 45.0%
! TTCCCCTGATTTCAGGTGTT+TGG + chr4.4:85674522-85674541 MS.gene052754:intron 45.0%
!! TAGAGTGTCGAAAAGGGAGA+GGG - chr4.4:85674779-85674798 None:intergenic 45.0%
!! TGTTTTTGCGGACAATGTCG+AGG - chr4.4:85674963-85674982 None:intergenic 45.0%
!! TTAGAGTGTCGAAAAGGGAG+AGG - chr4.4:85674780-85674799 None:intergenic 45.0%
!!! ACTCATTGCTTCGTCGGTTT+TGG + chr4.4:85674077-85674096 MS.gene052754:CDS 45.0%
!!! TTGCTTCGTCGGTTTTGGTT+TGG + chr4.4:85674082-85674101 MS.gene052754:CDS 45.0%
AAGTTCACCGGCCATGAACA+CGG + chr4.4:85674853-85674872 MS.gene052754:CDS 50.0%
AGATGAATGTCGGATGGGGA+TGG + chr4.4:85674109-85674128 MS.gene052754:CDS 50.0%
ATGAATGTCGGATGGGGATG+GGG + chr4.4:85674111-85674130 MS.gene052754:CDS 50.0%
CAGTAGAGACAGAATCCGAC+CGG + chr4.4:85674554-85674573 MS.gene052754:CDS 50.0%
CCGGTGAACTTTCTTGCGAA+AGG - chr4.4:85674844-85674863 None:intergenic 50.0%
CGCCACGATCATGGAAATGA+TGG - chr4.4:85674920-85674939 None:intergenic 50.0%
CGTTCGTTTACTCCCCGTTT+GGG + chr4.4:85674642-85674661 MS.gene052754:CDS 50.0%
CTATCAGTGATGCCCAAACG+GGG - chr4.4:85674657-85674676 None:intergenic 50.0%
CTCGTAGACGTAGGCACATT+TGG + chr4.4:85673309-85673328 MS.gene052754:intron 50.0%
CTGCATTTCCAAGCCCGTAT+CGG - chr4.4:85673508-85673527 None:intergenic 50.0%
CTGGTGGAAGTCCTCTTACA+AGG + chr4.4:85674214-85674233 MS.gene052754:CDS 50.0%
CTTGTAAGAGGACTTCCACC+AGG - chr4.4:85674216-85674235 None:intergenic 50.0%
GATGAATGTCGGATGGGGAT+GGG + chr4.4:85674110-85674129 MS.gene052754:CDS 50.0%
GCACCTACGAGGAAATACTG+CGG - chr4.4:85675033-85675052 None:intergenic 50.0%
GCGATAACCATTGCAACTCC+AGG - chr4.4:85674147-85674166 None:intergenic 50.0%
GCTATCAGTGATGCCCAAAC+GGG - chr4.4:85674658-85674677 None:intergenic 50.0%
GCTGTGTGAATCCGTGTTCA+TGG - chr4.4:85674867-85674886 None:intergenic 50.0%
GGCTATCAGTGATGCCCAAA+CGG - chr4.4:85674659-85674678 None:intergenic 50.0%
GTACCGCAGTATTTCCTCGT+AGG + chr4.4:85675027-85675046 MS.gene052754:CDS 50.0%
TCATTTCCATGATCGTGGCG+GGG + chr4.4:85674920-85674939 MS.gene052754:CDS 50.0%
TCGTTCGTTTACTCCCCGTT+TGG + chr4.4:85674641-85674660 MS.gene052754:CDS 50.0%
TGTGAATCCGTGTTCATGGC+CGG - chr4.4:85674863-85674882 None:intergenic 50.0%
TTGCGAAAGGGACGATGAGA+CGG - chr4.4:85674831-85674850 None:intergenic 50.0%
! TGGAGTACCTGGAGTTGCAA+TGG + chr4.4:85674137-85674156 MS.gene052754:CDS 50.0%
! TTTTGCGGACAATGTCGAGG+CGG - chr4.4:85674960-85674979 None:intergenic 50.0%
AAAAGGGAGAGGGAGGCTGA+TGG - chr4.4:85674769-85674788 None:intergenic 55.0%
AAGTGTCATCTTCTGGGCCC+CGG + chr4.4:85674798-85674817 MS.gene052754:CDS 55.0%
ACGATGAGACGGTCGTAGAC+CGG - chr4.4:85674820-85674839 None:intergenic 55.0%
CACTCTGCACTCGTAGACGT+AGG + chr4.4:85673300-85673319 MS.gene052754:intron 55.0%
CAGTTTGTCCTGACGTCGGA+TGG - chr4.4:85673894-85673913 None:intergenic 55.0%
CGGTGCACTCATTGCTTCGT+CGG + chr4.4:85674071-85674090 MS.gene052754:CDS 55.0%
CTACGTCTACGAGTGCAGAG+TGG - chr4.4:85673302-85673321 None:intergenic 55.0%
CTTGAAGACCGATACGGGCT+TGG + chr4.4:85673497-85673516 MS.gene052754:CDS 55.0%
GCTGCAGTTTGTCCTGACGT+CGG - chr4.4:85673898-85673917 None:intergenic 55.0%
GTGCAGAGCCTCCATGGATT+CGG - chr4.4:85674592-85674611 None:intergenic 55.0%
GTTACTGTGCAGAGCCTCCA+TGG - chr4.4:85674598-85674617 None:intergenic 55.0%
TAGAATCCCCGCCACGATCA+TGG - chr4.4:85674929-85674948 None:intergenic 55.0%
TTGATTGCGCTTGGAACCGG+CGG + chr4.4:85673937-85673956 MS.gene052754:CDS 55.0%
! AGTGTCGAAAAGGGAGAGGG+AGG - chr4.4:85674776-85674795 None:intergenic 55.0%
!!! GGATGGGGTTTTGGAGTACC+TGG + chr4.4:85674126-85674145 MS.gene052754:CDS 55.0%
AAACTTCGGCGGCACCTACG+AGG - chr4.4:85675044-85675063 None:intergenic 60.0%
CGATGAGACGGTCGTAGACC+GGG - chr4.4:85674819-85674838 None:intergenic 60.0%
GATGAGACGGTCGTAGACCG+GGG - chr4.4:85674818-85674837 None:intergenic 60.0%
! AATGGGTGCCATCCGACGTC+AGG + chr4.4:85673883-85673902 MS.gene052754:CDS 60.0%
! GTCGGATGGGGATGGGGTTT+TGG + chr4.4:85674117-85674136 MS.gene052754:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 85673033 85675282 85673033 ID=MS.gene052754
chr4.4 mRNA 85673033 85675282 85673033 ID=MS.gene052754.t1;Parent=MS.gene052754
chr4.4 exon 85673033 85673168 85673033 ID=MS.gene052754.t1.exon1;Parent=MS.gene052754.t1
chr4.4 CDS 85673033 85673168 85673033 ID=cds.MS.gene052754.t1;Parent=MS.gene052754.t1
chr4.4 exon 85673438 85673655 85673438 ID=MS.gene052754.t1.exon2;Parent=MS.gene052754.t1
chr4.4 CDS 85673438 85673655 85673438 ID=cds.MS.gene052754.t1;Parent=MS.gene052754.t1
chr4.4 exon 85673823 85674373 85673823 ID=MS.gene052754.t1.exon3;Parent=MS.gene052754.t1
chr4.4 CDS 85673823 85674373 85673823 ID=cds.MS.gene052754.t1;Parent=MS.gene052754.t1
chr4.4 exon 85674537 85675048 85674537 ID=MS.gene052754.t1.exon4;Parent=MS.gene052754.t1
chr4.4 CDS 85674537 85675048 85674537 ID=cds.MS.gene052754.t1;Parent=MS.gene052754.t1
chr4.4 exon 85675251 85675282 85675251 ID=MS.gene052754.t1.exon5;Parent=MS.gene052754.t1
chr4.4 CDS 85675251 85675282 85675251 ID=cds.MS.gene052754.t1;Parent=MS.gene052754.t1
Gene Sequence

>MS.gene052754

ATGGAAGATGTCAATATAAAAGCTGGTGTGAATGAAGAAGATGGTTTATATACAGAAGATGGAACAATTGACATTCACAAAAAACGTGCCAACAAGAAAAAGACAGGAAATTGGAAAGCATGCCGCTTTATTCTCGGTATTAAATTCTTAGTTTTATATCTTTCATAAACTTCAATGTCATATCCGATATATATTCCTGGACCGTAGTATTTAAACACAAGTTGTACATTATAACTCTAAGCTTGAATAATAGAAATGTACTGCAACCACTCTGCACTCGTAGACGTAGGCACATTTGGTCGTGTGTTCTCTGTTCGCTTTTTTAGTATATACTTGCTCGTCAACCAGCGTTGTTGTACTAACAATTCTTGTACTAGAATTTGATCACACGAAAAATTGTTGCAGGAAATGAATGTTGTGAAAGACTGGCTTACTATGGCATGAGTACAAACTTGGTGAACTATCTTGAAGACCGATACGGGCTTGGAAATGCAGCTGCTGCAAATACTGTCACAACTTGGTCGGGAACATGTTACATCACACCATTGCTTGGAGCCTTTTTAGCTGATGCATACATGGGAAGATACTGGACAATTGCTAGTTTCTCAAGTATCTATGTCATTGTAAGCTCATTTGAAGATAAGATTTGCTTTTTTGTTGAAATGTTTTGATAAAGGCATTTAATCTGCATTCTTTTCAATATCAGTTCATATTTAAATGTCTGAGGTTCATAATCAAAATGTCTGAGGGAAAGACATGTTAATGGAGTCATTGTTTGTGTGTTTTGCAGGGAATGGCGCTTTTAACATTTTCCGCTATTGCACCTGGACTAAAGCCTACATGTGATACTAATGGGTGCCATCCGACGTCAGGACAAACTGCAGCCCTTTTTACAACACTCTACTTGATTGCGCTTGGAACCGGCGGTATCAAACCATGTGTTTCATCTTTTGGTGCTGACCAATTTGATGAAAACGATGAGACTGAGAGGAAAAAGAAAAGCTCTTTTTTCAATTGGTTTTACTTCTCAATTAATATCGGTGCACTCATTGCTTCGTCGGTTTTGGTTTGGATACAGATGAATGTCGGATGGGGATGGGGTTTTGGAGTACCTGGAGTTGCAATGGTTATCGCCATTATATTTTTCTTCATCGGTAGTAGATGGTATAGACTTCAAATACCTGGTGGAAGTCCTCTTACAAGGATTTGTCAAGTTATTGTTGCAGCATTTAGGAAACTTGGGCAACAAGTTCCAGATGATAAGTCTCTTCTTTATGAGACTACTGATGTAGAATCTAACATCAAAGGGAGCCGGAAACTTGAACACACAAATGAATTGAAGTAAGATATCAAAACCATTTTTATATTTATCTTTAAGAGACTACTCATTGGCATGTCCATTTCTTATTCTAAACTTCATAATTCATTAACTCTAAAGTTTTTTATTTTACATTCTTATCTTACATTATAATAATAACTAACAAAGAAATTCCCCTGATTTCAGGTGTTTGGATAAGGCAGCAGTAGAGACAGAATCCGACCGGATAAAAGATTTACCGAATCCATGGAGGCTCTGCACAGTAACACAAGTCGAAGAACTCAAATCAATCGTTCGTTTACTCCCCGTTTGGGCATCACTGATAGCCTTTGCAACGGTATACAGTCAAATGGGAACCATGTTTGTCTTACAAGGCAACACAATGGATCAACACATTGGTCCTAAATTCAAAATTCCATCAGCCTCCCTCTCCCTTTTCGACACTCTAAGTGTCATCTTCTGGGCCCCGGTCTACGACCGTCTCATCGTCCCTTTCGCAAGAAAGTTCACCGGCCATGAACACGGATTCACACAGCTTCATAGAATCGGAATTGGCCTAGTCATCTCCATCATTTCCATGATCGTGGCGGGGATTCTAGAAGTCATCCGCCTCGACATTGTCCGCAAAAACAACTACTATGATCTCGAGACCATCCCTATGTCAATCTTCTGGCAAGTACCGCAGTATTTCCTCGTAGGTGCCGCCGAAGTTTTCACTAATATCGGTCAGATGGAGTTCTTTTATGGTCAGGCACCTGATGCTATGAGAAGTTTATGTGCTGCACTTTCACTTACAACTAATGCATTGGGAAATTATGTGAGTACATTGCTTGTTACTATTGTCACAAAAGTTACTACAAGAAATGGAAGTCTTGGTTGGATTCCAGATAATATGAATAGGGGTCATCTTGATTACTTCTACTGGCTTTTGA
Protein sequence

>MS.gene052754.t1

MEDVNIKAGVNEEDGLYTEDGTIDIHKKRANKKKTGNWKACRFILGNECCERLAYYGMSTNLVNYLEDRYGLGNAAAANTVTTWSGTCYITPLLGAFLADAYMGRYWTIASFSSIYVIGMALLTFSAIAPGLKPTCDTNGCHPTSGQTAALFTTLYLIALGTGGIKPCVSSFGADQFDENDETERKKKSSFFNWFYFSINIGALIASSVLVWIQMNVGWGWGFGVPGVAMVIAIIFFFIGSRWYRLQIPGGSPLTRICQVIVAAFRKLGQQVPDDKSLLYETTDVESNIKGSRKLEHTNELKCLDKAAVETESDRIKDLPNPWRLCTVTQVEELKSIVRLLPVWASLIAFATVYSQMGTMFVLQGNTMDQHIGPKFKIPSASLSLFDTLSVIFWAPVYDRLIVPFARKFTGHEHGFTQLHRIGIGLVISIISMIVAGILEVIRLDIVRKNNYYDLETIPMSIFWQVPQYFLVGVILITSTGF