AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33492

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33492.t1 MTR_4g015460 92.654 422 29 2 1 420 85 506 0.0 808
MS.gene33492.t1 MTR_4g015420 82.692 416 69 2 2 414 109 524 0.0 720
MS.gene33492.t1 MTR_4g066330 68.510 416 119 7 2 414 86 492 0.0 570
MS.gene33492.t1 MTR_2g043200 67.874 414 128 2 2 414 109 518 0.0 570
MS.gene33492.t1 MTR_4g066280 66.827 416 126 8 2 414 86 492 0.0 558
MS.gene33492.t1 MTR_2g098520 59.502 442 141 8 2 415 79 510 0.0 523
MS.gene33492.t1 MTR_2g098540 58.145 442 147 7 2 415 32 463 1.05e-179 509
MS.gene33492.t1 MTR_2g098540 58.145 442 147 7 2 415 76 507 1.25e-179 511
MS.gene33492.t1 MTR_2g098790 58.050 441 147 8 2 414 83 513 1.09e-178 508
MS.gene33492.t1 MTR_4g066340 58.239 443 147 7 2 416 8 440 4.72e-177 501
MS.gene33492.t1 MTR_4g066580 57.823 441 148 7 2 414 84 514 4.05e-175 500
MS.gene33492.t1 MTR_4g066210 56.916 441 152 8 2 414 88 518 1.27e-174 498
MS.gene33492.t1 MTR_4g066240 55.530 443 159 6 2 416 81 513 1.73e-174 498
MS.gene33492.t1 MTR_3g028720 57.823 441 148 7 2 414 83 513 1.04e-173 496
MS.gene33492.t1 MTR_4g066240 55.405 444 159 7 2 416 81 514 1.23e-172 493
MS.gene33492.t1 MTR_4g066590 57.500 440 149 7 2 413 78 507 2.03e-172 493
MS.gene33492.t1 MTR_6g078450 55.281 445 161 6 2 418 81 515 1.57e-171 490
MS.gene33492.t1 MTR_4g066640 55.782 441 159 8 2 416 84 514 2.43e-168 482
MS.gene33492.t1 MTR_6g078330 56.136 440 155 7 7 418 87 516 3.86e-167 479
MS.gene33492.t1 MTR_4g066630 55.227 440 159 6 2 413 84 513 8.82e-166 476
MS.gene33492.t1 MTR_6g078380 54.607 445 165 6 2 418 81 516 5.44e-160 461
MS.gene33492.t1 MTR_5g071520 54.486 457 149 7 2 415 84 524 5.79e-159 459
MS.gene33492.t1 MTR_6g078330 55.213 422 151 7 25 418 1 412 4.36e-157 450
MS.gene33492.t1 MTR_4g023000 51.606 436 167 8 7 414 74 493 4.67e-149 432
MS.gene33492.t1 MTR_3g026280 50.905 442 174 8 2 415 67 493 1.75e-147 428
MS.gene33492.t1 MTR_3g028720 56.848 387 129 7 2 360 83 459 7.49e-144 418
MS.gene33492.t1 MTR_6g078330 53.171 410 152 8 37 418 15 412 9.02e-144 416
MS.gene33492.t1 MTR_6g078450 54.522 387 138 6 2 360 81 457 9.04e-141 410
MS.gene33492.t1 MTR_3g026400 48.284 437 186 6 4 413 53 476 1.70e-137 402
MS.gene33492.t1 MTR_3g026400 48.284 437 186 6 4 413 84 507 3.17e-137 403
MS.gene33492.t1 MTR_4g066630 53.368 386 142 6 2 359 84 459 1.75e-135 396
MS.gene33492.t1 MTR_4g083000 45.824 443 198 7 2 414 72 502 4.14e-133 392
MS.gene33492.t1 MTR_3g026620 48.064 439 188 8 2 413 83 508 1.56e-131 388
MS.gene33492.t1 MTR_5g044000 44.977 438 198 8 7 413 95 520 1.32e-126 376
MS.gene33492.t1 MTR_4g066580 55.807 353 118 7 2 326 84 426 7.67e-126 371
MS.gene33492.t1 MTR_3g028720 55.524 353 119 7 2 326 83 425 2.02e-124 367
MS.gene33492.t1 MTR_4g081530 43.820 445 205 8 2 414 68 499 2.84e-122 365
MS.gene33492.t1 MTR_4g083000 44.762 420 190 7 25 414 1 408 4.49e-121 358
MS.gene33492.t1 MTR_4g066240 51.268 355 135 6 2 328 81 425 4.47e-120 356
MS.gene33492.t1 MTR_4g131810 42.568 444 212 10 1 413 72 503 5.10e-118 354
MS.gene33492.t1 MTR_4g066630 52.707 351 128 6 2 324 84 424 9.20e-118 351
MS.gene33492.t1 MTR_3g033790 43.155 431 214 7 2 414 63 480 2.38e-116 348
MS.gene33492.t1 MTR_3g033810 42.459 431 218 8 2 414 65 483 3.67e-114 343
MS.gene33492.t1 MTR_4g067040 53.097 339 121 7 2 312 79 407 8.13e-114 339
MS.gene33492.t1 MTR_2g022760 42.212 443 219 8 2 417 81 513 8.04e-112 338
MS.gene33492.t1 MTR_2g022710 40.625 448 226 10 2 420 79 515 1.76e-110 335
MS.gene33492.t1 MTR_7g033860 41.419 437 215 8 4 416 95 514 2.98e-110 334
MS.gene33492.t1 MTR_2g079580 41.270 441 219 10 1 413 75 503 9.70e-108 328
MS.gene33492.t1 MTR_0110s0040 42.282 447 210 12 1 417 86 514 1.02e-107 327
MS.gene33492.t1 MTR_4g067040 58.273 278 89 4 2 252 79 356 1.16e-107 323
MS.gene33492.t1 MTR_4g131800 43.311 441 213 8 4 417 562 992 1.14e-106 338
MS.gene33492.t1 MTR_3g028720 58.333 276 88 4 2 250 83 358 2.22e-106 320
MS.gene33492.t1 MTR_2g079550 40.816 441 221 10 1 413 73 501 3.38e-106 324
MS.gene33492.t1 MTR_0110s0030 40.541 444 222 10 1 417 86 514 6.75e-105 320
MS.gene33492.t1 MTR_4g066640 57.778 270 89 5 2 246 84 353 1.03e-102 310
MS.gene33492.t1 MTR_0108s0030 39.631 434 209 7 4 416 50 451 2.09e-102 311
MS.gene33492.t1 MTR_4g066630 55.396 278 97 4 2 252 84 361 1.02e-101 307
MS.gene33492.t1 MTR_2g022760 41.429 420 209 8 25 417 1 410 2.42e-100 305
MS.gene33492.t1 MTR_4g016920 40.138 436 210 8 7 413 76 489 1.70e-98 304
MS.gene33492.t1 MTR_2g022710 39.294 425 218 10 25 420 1 414 5.73e-98 299
MS.gene33492.t1 MTR_7g051650 41.324 438 216 12 1 413 111 532 1.28e-97 302
MS.gene33492.t1 MTR_7g051650 41.324 438 216 12 1 413 107 528 1.38e-97 302
MS.gene33492.t1 MTR_7g051610 40.984 427 215 8 12 416 123 534 4.88e-97 300
MS.gene33492.t1 MTR_6g078330 54.015 274 99 4 6 252 86 359 5.59e-97 295
MS.gene33492.t1 MTR_5g068740 37.783 442 225 7 5 417 72 492 1.02e-96 299
MS.gene33492.t1 MTR_7g051510 40.610 426 218 8 9 413 120 531 2.47e-96 299
MS.gene33492.t1 MTR_7g068910 38.215 437 221 8 7 415 80 495 3.70e-95 295
MS.gene33492.t1 MTR_1g088610 37.813 439 222 7 5 414 72 488 1.17e-94 297
MS.gene33492.t1 MTR_1g088610 39.167 120 66 2 299 417 503 616 9.53e-19 89.0
MS.gene33492.t1 MTR_7g068910 37.360 447 221 9 7 415 80 505 6.05e-92 287
MS.gene33492.t1 MTR_3g026400 51.481 270 103 4 4 247 84 351 4.21e-90 278
MS.gene33492.t1 MTR_7g068900 39.110 427 204 9 20 414 17 419 5.74e-90 281
MS.gene33492.t1 MTR_4g016920 39.234 418 203 8 25 413 1 396 5.46e-89 276
MS.gene33492.t1 MTR_7g056747 37.915 422 212 8 19 418 9 402 6.31e-88 273
MS.gene33492.t1 MTR_1g088630 36.199 442 207 9 5 417 40 435 6.33e-86 270
MS.gene33492.t1 MTR_2g022710 38.760 387 199 8 2 360 79 455 4.16e-85 268
MS.gene33492.t1 MTR_2g022710 38.760 387 199 8 2 360 79 455 9.14e-85 267
MS.gene33492.t1 MTR_2g022710 39.714 350 175 7 2 324 79 419 7.68e-81 255
MS.gene33492.t1 MTR_2g079580 40.341 352 171 9 1 324 75 415 1.85e-79 251
MS.gene33492.t1 MTR_2g022710 38.757 338 171 7 2 312 79 407 4.63e-75 240
MS.gene33492.t1 MTR_2g022710 40.065 307 152 6 2 281 79 380 9.75e-70 226
MS.gene33492.t1 MTR_0110s0040 43.060 281 130 6 1 253 86 364 1.43e-69 226
MS.gene33492.t1 MTR_4g016920 42.308 286 135 5 25 282 1 284 9.07e-66 213
MS.gene33492.t1 MTR_1g088630 41.573 267 102 6 5 243 72 312 2.23e-60 200
MS.gene33492.t1 MTR_1g088630 43.636 220 95 3 5 197 72 289 2.94e-54 182
MS.gene33492.t1 MTR_0110s0020 41.398 186 83 3 1 162 86 269 7.01e-44 155
MS.gene33492.t1 MTR_4g122980 27.465 426 210 13 21 418 183 537 2.39e-31 126
MS.gene33492.t1 MTR_5g069350 45.000 120 36 4 305 414 89 188 1.04e-20 89.7
MS.gene33492.t1 MTR_8g038650 42.697 89 50 1 162 249 62 150 9.25e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene33492.t1 AT5G44640 47.416 445 190 8 2 417 76 505 1.57e-139 409
MS.gene33492.t1 AT2G44450 48.423 444 186 9 2 417 76 504 2.18e-139 408
MS.gene33492.t1 AT5G42260 47.597 437 185 8 2 409 76 497 4.68e-136 400
MS.gene33492.t1 AT2G44480 45.928 442 202 6 2 416 80 511 4.47e-133 392
MS.gene33492.t1 AT2G44480 45.928 442 202 6 2 416 111 542 1.19e-132 392
MS.gene33492.t1 AT3G60130 45.928 442 195 7 2 414 75 501 2.14e-132 390
MS.gene33492.t1 AT1G26560 46.606 442 195 9 2 413 76 506 1.12e-130 386
MS.gene33492.t1 AT5G24550 45.701 442 198 8 7 416 81 512 2.90e-128 380
MS.gene33492.t1 AT3G60120 45.169 445 192 9 7 415 63 491 1.15e-127 379
MS.gene33492.t1 AT2G44490 42.729 447 216 7 2 417 59 496 2.73e-127 379
MS.gene33492.t1 AT3G60130 45.882 425 186 7 19 414 40 449 3.02e-127 375
MS.gene33492.t1 AT2G25630 45.455 440 181 8 2 413 75 483 1.21e-126 375
MS.gene33492.t1 AT5G54570 44.595 444 201 9 2 413 75 505 2.36e-126 376
MS.gene33492.t1 AT2G44480 45.585 419 191 6 25 416 1 409 1.10e-124 367
MS.gene33492.t1 AT5G36890 44.676 432 209 7 2 414 60 480 1.40e-124 370
MS.gene33492.t1 AT5G36890 44.676 432 209 7 2 414 60 480 2.12e-124 369
MS.gene33492.t1 AT3G60140 46.259 441 192 10 7 414 75 503 4.92e-124 371
MS.gene33492.t1 AT3G60130 45.047 424 180 7 19 414 40 438 3.81e-123 365
MS.gene33492.t1 AT1G51470 45.183 436 202 8 2 413 87 509 6.09e-123 366
MS.gene33492.t1 AT5G24540 45.103 439 205 8 7 416 81 512 7.88e-123 367
MS.gene33492.t1 AT1G47600 45.183 436 202 9 2 413 87 509 3.41e-122 364
MS.gene33492.t1 AT3G03640 44.191 439 205 7 7 414 84 513 3.43e-121 362
MS.gene33492.t1 AT1G47600 45.183 436 201 10 2 413 87 508 9.51e-121 360
MS.gene33492.t1 AT5G54570 44.893 421 187 9 25 413 1 408 8.26e-119 353
MS.gene33492.t1 AT1G75940 42.597 439 213 7 7 413 86 517 2.67e-116 350
MS.gene33492.t1 AT2G44460 44.977 438 203 7 6 414 77 505 2.23e-115 349
MS.gene33492.t1 AT3G09260 42.009 438 216 6 7 415 84 512 1.25e-113 343
MS.gene33492.t1 AT3G18080 43.052 439 203 10 8 416 89 510 4.05e-113 341
MS.gene33492.t1 AT1G66270 43.274 446 198 10 5 413 83 510 1.56e-112 340
MS.gene33492.t1 AT1G66280 43.280 439 208 8 5 413 83 510 3.09e-112 339
MS.gene33492.t1 AT2G44470 41.136 440 216 9 6 413 77 505 2.74e-111 339
MS.gene33492.t1 AT1G52400 41.818 440 213 9 7 414 88 516 3.54e-111 337
MS.gene33492.t1 AT1G52400 41.818 440 213 9 7 414 88 516 3.54e-111 337
MS.gene33492.t1 AT5G25980 42.955 440 211 12 4 414 95 523 9.99e-111 336
MS.gene33492.t1 AT2G32860 40.535 449 226 9 2 419 139 577 1.02e-110 338
MS.gene33492.t1 AT2G32860 39.868 454 221 11 2 419 139 576 2.23e-110 337
MS.gene33492.t1 AT1G66270 43.049 446 197 11 5 413 83 508 5.25e-110 333
MS.gene33492.t1 AT5G26000 42.141 439 215 8 4 413 83 511 2.51e-109 332
MS.gene33492.t1 AT1G61820 40.632 443 225 9 2 416 75 507 1.55e-108 330
MS.gene33492.t1 AT3G18070 41.928 446 204 12 2 417 74 494 2.33e-108 328
MS.gene33492.t1 AT3G18070 42.152 446 209 12 2 417 74 500 4.16e-108 328
MS.gene33492.t1 AT3G21370 41.403 442 214 9 7 414 82 512 4.18e-108 329
MS.gene33492.t1 AT5G28510 40.225 445 221 8 4 413 85 519 1.13e-107 328
MS.gene33492.t1 AT4G21760 40.449 445 223 11 2 419 98 527 1.24e-105 323
MS.gene33492.t1 AT2G44450 45.924 368 156 9 78 417 1 353 4.22e-104 313
MS.gene33492.t1 AT4G21760 39.865 444 217 11 2 419 98 517 1.57e-102 315
MS.gene33492.t1 AT5G54570 41.795 390 183 8 2 360 75 451 5.26e-100 305
MS.gene33492.t1 AT4G27830 38.621 435 218 9 7 413 71 484 9.45e-98 301
MS.gene33492.t1 AT1G61810 38.826 443 233 8 2 416 78 510 1.27e-97 301
MS.gene33492.t1 AT1G02850 39.679 436 211 10 8 414 74 486 2.87e-97 300
MS.gene33492.t1 AT3G62750 37.762 429 228 9 4 413 65 473 1.86e-96 298
MS.gene33492.t1 AT3G62750 37.762 429 228 9 4 413 91 499 5.16e-96 298
MS.gene33492.t1 AT5G24540 42.520 381 184 7 7 359 81 454 2.10e-95 294
MS.gene33492.t1 AT1G02850 37.691 459 211 10 8 414 74 509 4.97e-94 292
MS.gene33492.t1 AT4G27820 37.900 438 224 9 4 413 65 482 2.52e-93 290
MS.gene33492.t1 AT1G02850 37.609 460 211 11 8 414 74 510 3.47e-92 288
MS.gene33492.t1 AT1G61810 39.048 420 219 7 2 393 78 488 8.19e-92 287
MS.gene33492.t1 AT5G25980 41.667 384 186 10 4 360 95 467 1.76e-91 284
MS.gene33492.t1 AT5G25980 41.667 384 186 10 4 360 95 467 1.76e-91 284
MS.gene33492.t1 AT1G02850 38.621 435 190 10 8 414 74 459 7.69e-91 283
MS.gene33492.t1 AT3G60140 45.455 352 148 9 7 326 75 414 3.79e-90 280
MS.gene33492.t1 AT4G22100 38.444 437 224 10 7 416 67 485 6.09e-90 281
MS.gene33492.t1 AT3G62740 36.636 434 224 11 7 413 69 478 6.24e-90 281
MS.gene33492.t1 AT4G27830 37.890 417 210 9 25 413 1 396 1.76e-89 277
MS.gene33492.t1 AT1G61810 38.333 420 221 8 25 416 1 410 4.00e-89 276
MS.gene33492.t1 AT5G26000 40.365 384 192 6 4 360 83 456 1.15e-88 276
MS.gene33492.t1 AT2G44470 39.221 385 192 8 6 359 77 450 1.57e-87 273
MS.gene33492.t1 AT3G62750 36.765 408 219 9 25 413 1 388 2.65e-86 269
MS.gene33492.t1 AT3G62750 36.765 408 219 9 25 413 1 388 2.65e-86 269
MS.gene33492.t1 AT1G52400 40.104 384 188 8 7 359 88 460 1.19e-85 269
MS.gene33492.t1 AT1G60090 36.674 439 231 10 3 414 65 483 9.60e-85 268
MS.gene33492.t1 AT4G27820 36.930 417 215 9 25 413 1 397 2.21e-84 264
MS.gene33492.t1 AT4G27820 36.930 417 215 9 25 413 1 397 2.21e-84 264
MS.gene33492.t1 AT5G48375 40.933 386 169 11 4 378 74 411 4.02e-84 264
MS.gene33492.t1 AT1G45191 36.092 435 227 8 7 414 74 484 5.99e-83 263
MS.gene33492.t1 AT1G60090 35.955 445 233 11 3 414 65 490 3.12e-82 261
MS.gene33492.t1 AT1G61820 38.770 374 192 8 71 416 4 368 9.19e-82 256
MS.gene33492.t1 AT2G44460 42.391 368 174 7 76 414 1 359 9.40e-82 258
MS.gene33492.t1 AT1G45191 35.862 435 239 8 7 414 74 495 3.53e-81 259
MS.gene33492.t1 AT1G45191 35.862 435 239 8 7 414 86 507 4.48e-81 259
MS.gene33492.t1 AT1G45191 35.862 435 234 9 7 414 74 490 4.61e-81 258
MS.gene33492.t1 AT3G62750 36.605 377 201 8 4 362 91 447 2.17e-78 250
MS.gene33492.t1 AT2G44480 49.407 253 102 4 2 228 111 363 2.19e-77 245
MS.gene33492.t1 AT2G44470 43.750 272 125 5 6 249 77 348 4.47e-75 239
MS.gene33492.t1 AT3G62740 36.828 372 185 10 7 352 69 416 1.09e-74 239
MS.gene33492.t1 AT3G18070 40.580 345 160 10 103 417 94 423 5.19e-71 230
MS.gene33492.t1 AT3G62750 42.537 268 134 5 4 253 65 330 7.04e-71 228
MS.gene33492.t1 AT3G62750 42.537 268 134 5 4 253 65 330 7.04e-71 228
MS.gene33492.t1 AT2G44480 40.767 287 157 4 133 416 29 305 3.10e-70 224
MS.gene33492.t1 AT3G62750 42.537 268 134 5 4 253 91 356 5.81e-70 228
MS.gene33492.t1 AT1G02850 37.228 368 163 8 8 324 74 424 3.97e-69 225
MS.gene33492.t1 AT1G61810 36.182 351 187 7 2 324 78 419 4.97e-66 217
MS.gene33492.t1 AT3G18070 43.007 286 142 8 138 417 41 311 6.92e-66 213
MS.gene33492.t1 AT3G18070 41.606 274 130 6 2 249 74 343 8.09e-64 210
MS.gene33492.t1 AT1G60270 40.860 279 136 5 7 259 70 345 3.56e-61 203
MS.gene33492.t1 AT1G51490 38.796 299 156 5 2 277 62 356 5.20e-59 198
MS.gene33492.t1 AT1G45191 39.033 269 136 4 7 249 74 340 1.10e-58 197
MS.gene33492.t1 AT1G45191 39.033 269 136 4 7 249 74 340 1.16e-58 197
MS.gene33492.t1 AT1G60270 38.790 281 135 7 25 278 1 271 8.47e-53 178
MS.gene33492.t1 AT1G61810 39.286 224 110 3 2 200 78 300 2.12e-47 165
MS.gene33492.t1 AT3G18070 37.008 254 118 8 103 326 94 335 2.15e-38 142
MS.gene33492.t1 AT5G16580 33.219 292 152 10 103 370 27 299 5.97e-36 134
MS.gene33492.t1 AT3G18070 38.500 200 105 6 133 326 36 223 1.72e-33 125
MS.gene33492.t1 AT3G06510 25.917 436 240 11 20 418 159 548 5.46e-33 131
MS.gene33492.t1 AT3G06510 25.995 427 217 12 20 418 159 514 8.54e-31 125

Find 90 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAACATAATAGAATTAT+TGG 0.142938 4.2:+85980598 MS.gene33492:CDS
GATATATGCTTCAAGGAATT+TGG 0.182257 4.2:+85979066 MS.gene33492:CDS
GAGCAATCTAAGAGTCTTAT+TGG 0.231240 4.2:+85979508 MS.gene33492:CDS
ACTGTTAATGTGACTTTAAC+AGG 0.248704 4.2:+85979649 MS.gene33492:CDS
GGGGAAGATCCCAATGAAAA+AGG 0.264707 4.2:-85978523 None:intergenic
CAGGCTCAAAATGGTATAAT+AGG 0.277472 4.2:+85979222 MS.gene33492:CDS
ACGATTATCATAACAGAAAA+TGG 0.280720 4.2:+85979975 MS.gene33492:CDS
ATACTTTGCATGGTCCCTTT+TGG 0.290939 4.2:+85980462 MS.gene33492:CDS
TGTTTGTCCAAAGGGGATTC+AGG 0.305206 4.2:+85979914 MS.gene33492:CDS
AGCTCTCTGCCCGGTGGTTT+AGG 0.309537 4.2:+85980569 MS.gene33492:CDS
GACGGATACACTGTGCGTTT+TGG 0.311280 4.2:+85980502 MS.gene33492:CDS
GACGCCCTTGATTTCATGTT+TGG 0.322541 4.2:+85979312 MS.gene33492:CDS
ACCACATATCAGGCTCAAAA+TGG 0.345183 4.2:+85979213 MS.gene33492:CDS
TCTAATGGGTCAGTTGAGTA+TGG 0.349718 4.2:-85979273 None:intergenic
CATGAAATCAAGGGCGTCTT+CGG 0.355937 4.2:-85979307 None:intergenic
GCAGTGAACGTTGGTAAATC+TGG 0.366493 4.2:-85979484 None:intergenic
TGATCACTATCATCGTTATA+AGG 0.379609 4.2:+85977939 MS.gene33492:CDS
CAATATCCTTGGCCAAGATA+TGG 0.382965 4.2:-85978221 None:intergenic
GATCCAGACTGTATAGGAAT+TGG 0.386737 4.2:-85979592 None:intergenic
AGGCAAGGTGTGAATGTAAA+AGG 0.387936 4.2:+85980439 MS.gene33492:intron
CTATTACTTGGCAATATCCT+TGG 0.390771 4.2:-85978232 None:intergenic
TCCATTCAAAGTTGTCCAAA+AGG 0.405927 4.2:-85980477 None:intergenic
TGGCTATATGTTTGTCCAAA+GGG 0.410402 4.2:+85979906 MS.gene33492:CDS
CATACAGATTCTCCATATCT+TGG 0.418726 4.2:+85978209 MS.gene33492:CDS
TTTATACAGCCGCTAACAAC+AGG 0.426920 4.2:+85979430 MS.gene33492:CDS
ATACTCATCTTGTAAGGCCT+GGG 0.427696 4.2:-85978543 None:intergenic
ATCAATGAGCTACTTGCTAA+TGG 0.429019 4.2:+85978392 MS.gene33492:CDS
CAGGCCTTACAAGATGAGTA+TGG 0.435383 4.2:+85978545 MS.gene33492:CDS
ATTGTATAAAACCACATATC+AGG 0.437772 4.2:+85979203 MS.gene33492:CDS
ACATAAGACACCAAGCTTGC+TGG 0.447607 4.2:-85979460 None:intergenic
AACCACCGGGCAGAGAGCTT+AGG 0.450563 4.2:-85980565 None:intergenic
CATACTCATCTTGTAAGGCC+TGG 0.455210 4.2:-85978544 None:intergenic
ATGACAGATAAGAATAAAGA+TGG 0.458312 4.2:+85979782 MS.gene33492:intron
CCGCCTGATCCAGACTGTAT+AGG 0.458956 4.2:-85979598 None:intergenic
TAGCAAGTAGCTCATTGATC+AGG 0.461889 4.2:-85978388 None:intergenic
ACACTTTGAATACATCAGAT+TGG 0.462336 4.2:+85979133 MS.gene33492:CDS
ACCATCCAAACATGAAATCA+AGG 0.473106 4.2:-85979317 None:intergenic
CTTCGTGGATATATGCTTCA+AGG 0.473903 4.2:+85979059 MS.gene33492:CDS
ACCACCGGGCAGAGAGCTTA+GGG 0.475465 4.2:-85980564 None:intergenic
ATCACTCGAAGAGGCGCTCA+TGG 0.478154 4.2:+85980244 MS.gene33492:CDS
TTGGAGACAGAGTAAAGCAT+TGG 0.480355 4.2:+85979085 MS.gene33492:CDS
AATAAAGATGGGACATACAT+TGG 0.483048 4.2:+85979794 MS.gene33492:CDS
ATTCCTATACAGTCTGGATC+AGG 0.483543 4.2:+85979595 MS.gene33492:CDS
AGTAGGTCCTGAATCCCCTT+TGG 0.484753 4.2:-85979921 None:intergenic
CAAAATGGTATAATAGGCAT+TGG 0.486147 4.2:+85979228 MS.gene33492:CDS
GCATTGGATTAAACTCTCAC+TGG 0.490284 4.2:+85979244 MS.gene33492:CDS
AGCAATCTAAGAGTCTTATT+GGG 0.490733 4.2:+85979509 MS.gene33492:CDS
GCCTCCATACTCATCTTGTA+AGG 0.497866 4.2:-85978549 None:intergenic
CCATTCAAAGTTGTCCAAAA+GGG 0.499789 4.2:-85980476 None:intergenic
CCCTTGATTTCATGTTTGGA+TGG 0.504970 4.2:+85979316 MS.gene33492:CDS
TGGAGACAGAGTAAAGCATT+GGG 0.508305 4.2:+85979086 MS.gene33492:CDS
TCTGGATCAGGCGGCGCTGA+CGG 0.508743 4.2:+85979607 MS.gene33492:CDS
CAGCTGCATGAGCAAGAAGT+TGG 0.509222 4.2:-85979173 None:intergenic
AGGAATTGGTTTCGTTGCAT+TGG 0.510612 4.2:-85979578 None:intergenic
AAACCAATTCCTATACAGTC+TGG 0.511299 4.2:+85979589 MS.gene33492:CDS
AGGAAAGTATCCAGCAAGCT+TGG 0.526086 4.2:+85979450 MS.gene33492:CDS
GACAACTTTGAATGGAATGA+CGG 0.527539 4.2:+85980484 MS.gene33492:CDS
GGATACTTTCCTGTTGTTAG+CGG 0.535598 4.2:-85979439 None:intergenic
GAAGTTGGTTGTGTGTGGCC+TGG 0.537777 4.2:-85979158 None:intergenic
AATATGTGCAGGCTGCAACT+TGG 0.539391 4.2:+85979886 MS.gene33492:intron
CAAGAAACTTCCTAAACCAC+CGG 0.545200 4.2:-85980579 None:intergenic
TTGGCTATATGTTTGTCCAA+AGG 0.547740 4.2:+85979905 MS.gene33492:CDS
TTTCCTTTGTGTACAACAGT+AGG 0.554600 4.2:-85979938 None:intergenic
GTATTCAAAGTGTAAGAGAA+TGG 0.570526 4.2:-85979123 None:intergenic
GATTCTCCATATCTTGGCCA+AGG 0.571513 4.2:+85978215 MS.gene33492:CDS
GACACCCTAAGCTCTCTGCC+CGG 0.573913 4.2:+85980560 MS.gene33492:CDS
ACTGCATACAGGGATGGTAA+AGG 0.575949 4.2:+85977891 None:intergenic
TGGGACATACATTGGAGCAT+GGG 0.581493 4.2:+85979802 MS.gene33492:CDS
ATGGGACATACATTGGAGCA+TGG 0.582897 4.2:+85979801 MS.gene33492:CDS
TGTAAAATATGATTCCTGCT+TGG 0.587277 4.2:-85978361 None:intergenic
GAATACATCAGATTGGTACC+AGG 0.597455 4.2:+85979140 MS.gene33492:CDS
GATTGCTCTGCAGTGAACGT+TGG 0.602380 4.2:-85979493 None:intergenic
GGCTATATGTTTGTCCAAAG+GGG 0.602874 4.2:+85979907 MS.gene33492:CDS
CATACAGGGATGGTAAAGGA+TGG 0.607856 4.2:+85977895 None:intergenic
TGACAGATAAGAATAAAGAT+GGG 0.612129 4.2:+85979783 MS.gene33492:intron
ATGTAAAAGGATACTTTGCA+TGG 0.622027 4.2:+85980452 MS.gene33492:CDS
AGTGGCGGTGTAAACCAAGC+AGG 0.626808 4.2:+85978347 MS.gene33492:CDS
AGCAAGAAGTTGGTTGTGTG+TGG 0.636210 4.2:-85979163 None:intergenic
CCATCCAAACATGAAATCAA+GGG 0.640294 4.2:-85979316 None:intergenic
TACTCATCTTGTAAGGCCTG+GGG 0.645812 4.2:-85978542 None:intergenic
TATGCAGATGGAAAACTAAG+TGG 0.646823 4.2:+85978329 MS.gene33492:intron
TGCATTGGCAGCATACATGC+TGG 0.650774 4.2:-85979563 None:intergenic
CCTATACAGTCTGGATCAGG+CGG 0.652705 4.2:+85979598 MS.gene33492:CDS
TCCTACACTATCACTCGAAG+AGG 0.658030 4.2:+85980235 MS.gene33492:CDS
GGACCTACTGTTGTACACAA+AGG 0.659998 4.2:+85979935 MS.gene33492:CDS
AAGAAACTTCCTAAACCACC+GGG 0.661886 4.2:-85980578 None:intergenic
ACCCTAAGCTCTCTGCCCGG+TGG 0.683098 4.2:+85980563 MS.gene33492:CDS
GCCTTACAAGATGAGTATGG+AGG 0.687554 4.2:+85978548 MS.gene33492:CDS
GCAGATGGAAAACTAAGTGG+CGG 0.693644 4.2:+85978332 MS.gene33492:intron
GCCTCTTCGAGTGATAGTGT+AGG 0.709813 4.2:-85980236 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAAAAATATATTTCAATA+CGG - chr4.2:85979400-85979419 None:intergenic 10.0%
!! TTCTAATAAATTTAAATCAT+AGG + chr4.2:85980194-85980213 MS.gene33492:intron 10.0%
!! ATTCAAGAAATAATAAATTG+AGG + chr4.2:85978962-85978981 MS.gene33492:intron 15.0%
!! TATGATATACTAATAAAGTA+TGG - chr4.2:85978432-85978451 None:intergenic 15.0%
!! TTCATAATAATACAAATTGA+TGG + chr4.2:85978467-85978486 MS.gene33492:intron 15.0%
!!! AAATAATTTTAGTGATCAAA+AGG + chr4.2:85978045-85978064 MS.gene33492:intron 15.0%
!!! ATATATTTTTATCTGTTTAC+AGG + chr4.2:85979407-85979426 MS.gene33492:intron 15.0%
!!! ATTAAACATTTTCTATTACT+TGG - chr4.2:85978247-85978266 None:intergenic 15.0%
!! AAAACAATTTATGTCAAACA+AGG - chr4.2:85979750-85979769 None:intergenic 20.0%
!! ATTCTGATTTAAGTAAATAG+AGG - chr4.2:85980335-85980354 None:intergenic 20.0%
!! CTTGAACATAATAGAATTAT+TGG + chr4.2:85980598-85980617 MS.gene33492:CDS 20.0%
!! GAAATTATATATCACATTAG+AGG - chr4.2:85977967-85977986 None:intergenic 20.0%
!! TTCCTATTAAGATGTAAAAA+TGG - chr4.2:85978155-85978174 None:intergenic 20.0%
!! TTCTGATTTAAGTAAATAGA+GGG - chr4.2:85980334-85980353 None:intergenic 20.0%
!!! AACCATTTTTACATCTTAAT+AGG + chr4.2:85978150-85978169 MS.gene33492:intron 20.0%
!!! TTGACTATATTGTTGTTTTT+TGG + chr4.2:85980404-85980423 MS.gene33492:intron 20.0%
! ACGATTATCATAACAGAAAA+TGG + chr4.2:85979975-85979994 MS.gene33492:CDS 25.0%
! ATGACAGATAAGAATAAAGA+TGG + chr4.2:85979782-85979801 MS.gene33492:intron 25.0%
! ATTCTAATGTTAGTTAGTTG+TGG - chr4.2:85978916-85978935 None:intergenic 25.0%
! ATTGTATAAAACCACATATC+AGG + chr4.2:85979203-85979222 MS.gene33492:CDS 25.0%
! GTTTATTAGACACTGAAAAA+AGG - chr4.2:85980161-85980180 None:intergenic 25.0%
! TGACAGATAAGAATAAAGAT+GGG + chr4.2:85979783-85979802 MS.gene33492:intron 25.0%
!! AATATGCAGAGATGATTTTA+AGG + chr4.2:85979035-85979054 MS.gene33492:intron 25.0%
!! ATTATCTTTTATCTGCAATG+AGG + chr4.2:85980303-85980322 MS.gene33492:CDS 25.0%
!! ATTGGGTCATATGATTTTAT+TGG + chr4.2:85979526-85979545 MS.gene33492:CDS 25.0%
!! GTTTGACATAAATTGTTTTC+TGG + chr4.2:85979751-85979770 MS.gene33492:intron 25.0%
!! TTTTCTGTTATGATAATCGT+AGG - chr4.2:85979975-85979994 None:intergenic 25.0%
!!! TTTTTTGGTTTTTTGTGTGA+AGG + chr4.2:85980419-85980438 MS.gene33492:intron 25.0%
AAATCAACTATGTTGTTGGT+TGG - chr4.2:85978891-85978910 None:intergenic 30.0%
AACAACTAAACAATATGTGC+AGG + chr4.2:85979875-85979894 MS.gene33492:intron 30.0%
AACTCAAACAAATATGCAGA+TGG + chr4.2:85978317-85978336 MS.gene33492:intron 30.0%
AATAAAGATGGGACATACAT+TGG + chr4.2:85979794-85979813 MS.gene33492:CDS 30.0%
ACACTTTGAATACATCAGAT+TGG + chr4.2:85979133-85979152 MS.gene33492:CDS 30.0%
ACTGTTAATGTGACTTTAAC+AGG + chr4.2:85979649-85979668 MS.gene33492:CDS 30.0%
ATGTAAAAGGATACTTTGCA+TGG + chr4.2:85980452-85980471 MS.gene33492:CDS 30.0%
ATTGATGGAATTATTGATCC+AGG + chr4.2:85978482-85978501 MS.gene33492:intron 30.0%
CAAAATGGTATAATAGGCAT+TGG + chr4.2:85979228-85979247 MS.gene33492:CDS 30.0%
GATATATGCTTCAAGGAATT+TGG + chr4.2:85979066-85979085 MS.gene33492:CDS 30.0%
GGATAAATCAACTATGTTGT+TGG - chr4.2:85978895-85978914 None:intergenic 30.0%
GTATTCAAAGTGTAAGAGAA+TGG - chr4.2:85979126-85979145 None:intergenic 30.0%
TGATCACTATCATCGTTATA+AGG + chr4.2:85977939-85977958 MS.gene33492:CDS 30.0%
TGTAAAATATGATTCCTGCT+TGG - chr4.2:85978364-85978383 None:intergenic 30.0%
TTAGTTAGTTAGTTGGTTAG+TGG - chr4.2:85978868-85978887 None:intergenic 30.0%
! AGAGAATGATTAGAACGTTT+TGG + chr4.2:85978639-85978658 MS.gene33492:intron 30.0%
! CTTTAGAGAGAAAACTACAA+AGG + chr4.2:85978713-85978732 MS.gene33492:intron 30.0%
!! AGCAATCTAAGAGTCTTATT+GGG + chr4.2:85979509-85979528 MS.gene33492:CDS 30.0%
!! ATATGTGACCCTTTTTCATT+GGG + chr4.2:85978514-85978533 MS.gene33492:CDS 30.0%
AAACCAATTCCTATACAGTC+TGG + chr4.2:85979589-85979608 MS.gene33492:CDS 35.0%
ACCATCCAAACATGAAATCA+AGG - chr4.2:85979320-85979339 None:intergenic 35.0%
ATCCATGGTTGTTGTAGAAA+CGG - chr4.2:85979361-85979380 None:intergenic 35.0%
CAATGAAAAAGGGTCACATA+TGG - chr4.2:85978515-85978534 None:intergenic 35.0%
CAGGCTCAAAATGGTATAAT+AGG + chr4.2:85979222-85979241 MS.gene33492:CDS 35.0%
CATACAGATTCTCCATATCT+TGG + chr4.2:85978209-85978228 MS.gene33492:CDS 35.0%
CCATCCAAACATGAAATCAA+GGG - chr4.2:85979319-85979338 None:intergenic 35.0%
CCATTCAAAGTTGTCCAAAA+GGG - chr4.2:85980479-85980498 None:intergenic 35.0%
CTATTACTTGGCAATATCCT+TGG - chr4.2:85978235-85978254 None:intergenic 35.0%
GACAACTTTGAATGGAATGA+CGG + chr4.2:85980484-85980503 MS.gene33492:CDS 35.0%
TATGCAGATGGAAAACTAAG+TGG + chr4.2:85978329-85978348 MS.gene33492:intron 35.0%
TCCATTCAAAGTTGTCCAAA+AGG - chr4.2:85980480-85980499 None:intergenic 35.0%
TGATGTATGCCATCTTCATT+AGG - chr4.2:85979697-85979716 None:intergenic 35.0%
TGGCTATATGTTTGTCCAAA+GGG + chr4.2:85979906-85979925 MS.gene33492:CDS 35.0%
TTCATTAGGAGTGGTAAGAA+AGG - chr4.2:85979683-85979702 None:intergenic 35.0%
TTGGCTATATGTTTGTCCAA+AGG + chr4.2:85979905-85979924 MS.gene33492:CDS 35.0%
TTTCCTTTGTGTACAACAGT+AGG - chr4.2:85979941-85979960 None:intergenic 35.0%
! ATCAATGAGCTACTTGCTAA+TGG + chr4.2:85978392-85978411 MS.gene33492:CDS 35.0%
! GAAACGGTCAAAAAGTATAG+TGG - chr4.2:85979345-85979364 None:intergenic 35.0%
! GAGCAATCTAAGAGTCTTAT+TGG + chr4.2:85979508-85979527 MS.gene33492:CDS 35.0%
! TGGTTGGTTAGTTAGTTAGT+TGG - chr4.2:85978875-85978894 None:intergenic 35.0%
!! AGATGATTTTAAGGACTTCG+TGG + chr4.2:85979044-85979063 MS.gene33492:CDS 35.0%
!! CATATGTGACCCTTTTTCAT+TGG + chr4.2:85978513-85978532 MS.gene33492:CDS 35.0%
!! GTTTGAGTTGTCAGTGTATA+TGG - chr4.2:85978306-85978325 None:intergenic 35.0%
AAGAAACTTCCTAAACCACC+GGG - chr4.2:85980581-85980600 None:intergenic 40.0%
ACCACATATCAGGCTCAAAA+TGG + chr4.2:85979213-85979232 MS.gene33492:CDS 40.0%
ACCATGGATCACGACAAAAT+TGG + chr4.2:85979372-85979391 MS.gene33492:intron 40.0%
AGGCAAGGTGTGAATGTAAA+AGG + chr4.2:85980439-85980458 MS.gene33492:intron 40.0%
ATACTCATCTTGTAAGGCCT+GGG - chr4.2:85978546-85978565 None:intergenic 40.0%
ATTCCTATACAGTCTGGATC+AGG + chr4.2:85979595-85979614 MS.gene33492:CDS 40.0%
CAAGAAACTTCCTAAACCAC+CGG - chr4.2:85980582-85980601 None:intergenic 40.0%
CAATATCCTTGGCCAAGATA+TGG - chr4.2:85978224-85978243 None:intergenic 40.0%
CCATGGATCACGACAAAATT+GGG + chr4.2:85979373-85979392 MS.gene33492:intron 40.0%
CCCTTGATTTCATGTTTGGA+TGG + chr4.2:85979316-85979335 MS.gene33492:CDS 40.0%
CTTACCACTCCTAATGAAGA+TGG + chr4.2:85979685-85979704 MS.gene33492:intron 40.0%
CTTCGTGGATATATGCTTCA+AGG + chr4.2:85979059-85979078 MS.gene33492:CDS 40.0%
GAATACATCAGATTGGTACC+AGG + chr4.2:85979140-85979159 MS.gene33492:CDS 40.0%
GATCCAGACTGTATAGGAAT+TGG - chr4.2:85979595-85979614 None:intergenic 40.0%
GCATTGGATTAAACTCTCAC+TGG + chr4.2:85979244-85979263 MS.gene33492:CDS 40.0%
GGATACTTTCCTGTTGTTAG+CGG - chr4.2:85979442-85979461 None:intergenic 40.0%
GGCTATATGTTTGTCCAAAG+GGG + chr4.2:85979907-85979926 MS.gene33492:CDS 40.0%
GGGAAGATCCCAATGAAAAA+GGG - chr4.2:85978525-85978544 None:intergenic 40.0%
GTCACATATGGTATTTGACC+TGG - chr4.2:85978503-85978522 None:intergenic 40.0%
TAGCAAGTAGCTCATTGATC+AGG - chr4.2:85978391-85978410 None:intergenic 40.0%
TATGCCATCTTCATTAGGAG+TGG - chr4.2:85979692-85979711 None:intergenic 40.0%
TTGGAGACAGAGTAAAGCAT+TGG + chr4.2:85979085-85979104 MS.gene33492:CDS 40.0%
TTTATACAGCCGCTAACAAC+AGG + chr4.2:85979430-85979449 MS.gene33492:CDS 40.0%
! ACCATTTTGAGCCTGATATG+TGG - chr4.2:85979217-85979236 None:intergenic 40.0%
! AGGAATTGGTTTCGTTGCAT+TGG - chr4.2:85979581-85979600 None:intergenic 40.0%
! ATACTTTGCATGGTCCCTTT+TGG + chr4.2:85980462-85980481 MS.gene33492:CDS 40.0%
! CCCTTTTGGACAACTTTGAA+TGG + chr4.2:85980476-85980495 MS.gene33492:CDS 40.0%
! CGGCAGCTTTTAGATCTAAT+GGG - chr4.2:85979290-85979309 None:intergenic 40.0%
! TCGGCAGCTTTTAGATCTAA+TGG - chr4.2:85979291-85979310 None:intergenic 40.0%
! TCTAATGGGTCAGTTGAGTA+TGG - chr4.2:85979276-85979295 None:intergenic 40.0%
! TGGAGACAGAGTAAAGCATT+GGG + chr4.2:85979086-85979105 MS.gene33492:CDS 40.0%
!! CTCTCTAAAGTGTGAGTGTA+TGG - chr4.2:85978703-85978722 None:intergenic 40.0%
!!! TGGTTTTTTGTGTGAAGGCA+AGG + chr4.2:85980424-85980443 MS.gene33492:intron 40.0%
AATATGTGCAGGCTGCAACT+TGG + chr4.2:85979886-85979905 MS.gene33492:intron 45.0%
ACATAAGACACCAAGCTTGC+TGG - chr4.2:85979463-85979482 None:intergenic 45.0%
ATGGGACATACATTGGAGCA+TGG + chr4.2:85979801-85979820 MS.gene33492:CDS 45.0%
CAGGCCTTACAAGATGAGTA+TGG + chr4.2:85978545-85978564 MS.gene33492:CDS 45.0%
CATACTCATCTTGTAAGGCC+TGG - chr4.2:85978547-85978566 None:intergenic 45.0%
GACCGTTTCTACAACAACCA+TGG + chr4.2:85979356-85979375 MS.gene33492:intron 45.0%
GACGCCCTTGATTTCATGTT+TGG + chr4.2:85979312-85979331 MS.gene33492:CDS 45.0%
GATTCTCCATATCTTGGCCA+AGG + chr4.2:85978215-85978234 MS.gene33492:CDS 45.0%
GCAGATGGAAAACTAAGTGG+CGG + chr4.2:85978332-85978351 MS.gene33492:intron 45.0%
GCCTCCATACTCATCTTGTA+AGG - chr4.2:85978552-85978571 None:intergenic 45.0%
GCCTTACAAGATGAGTATGG+AGG + chr4.2:85978548-85978567 MS.gene33492:CDS 45.0%
GGACCTACTGTTGTACACAA+AGG + chr4.2:85979935-85979954 MS.gene33492:CDS 45.0%
GGGGAAGATCCCAATGAAAA+AGG - chr4.2:85978526-85978545 None:intergenic 45.0%
TACTCATCTTGTAAGGCCTG+GGG - chr4.2:85978545-85978564 None:intergenic 45.0%
TCCTACACTATCACTCGAAG+AGG + chr4.2:85980235-85980254 MS.gene33492:CDS 45.0%
TGGGACATACATTGGAGCAT+GGG + chr4.2:85979802-85979821 MS.gene33492:CDS 45.0%
TGTTTGTCCAAAGGGGATTC+AGG + chr4.2:85979914-85979933 MS.gene33492:CDS 45.0%
! AGCAAGAAGTTGGTTGTGTG+TGG - chr4.2:85979166-85979185 None:intergenic 45.0%
! CCCAATTTTGTCGTGATCCA+TGG - chr4.2:85979376-85979395 None:intergenic 45.0%
! GCAGTGAACGTTGGTAAATC+TGG - chr4.2:85979487-85979506 None:intergenic 45.0%
! TTTTCATTGGGATCTTCCCC+AGG + chr4.2:85978526-85978545 MS.gene33492:CDS 45.0%
!! AGGAAAGTATCCAGCAAGCT+TGG + chr4.2:85979450-85979469 MS.gene33492:CDS 45.0%
!! CATGAAATCAAGGGCGTCTT+CGG - chr4.2:85979310-85979329 None:intergenic 45.0%
!!! AAAATTATTTGATTTATTTA+AGG - chr4.2:85978035-85978054 None:intergenic 5.0%
AGTAGGTCCTGAATCCCCTT+TGG - chr4.2:85979924-85979943 None:intergenic 50.0%
CAGCTGCATGAGCAAGAAGT+TGG - chr4.2:85979176-85979195 None:intergenic 50.0%
CCTATACAGTCTGGATCAGG+CGG + chr4.2:85979598-85979617 MS.gene33492:CDS 50.0%
GATTGCTCTGCAGTGAACGT+TGG - chr4.2:85979496-85979515 None:intergenic 50.0%
GGGTGAGTAAGACTCTCTTG+AGG + chr4.2:85979822-85979841 MS.gene33492:intron 50.0%
! GACGGATACACTGTGCGTTT+TGG + chr4.2:85980502-85980521 MS.gene33492:CDS 50.0%
! GCCTCTTCGAGTGATAGTGT+AGG - chr4.2:85980239-85980258 None:intergenic 50.0%
! TGCATTGGCAGCATACATGC+TGG - chr4.2:85979566-85979585 None:intergenic 50.0%
AGTGGCGGTGTAAACCAAGC+AGG + chr4.2:85978347-85978366 MS.gene33492:CDS 55.0%
CCGCCTGATCCAGACTGTAT+AGG - chr4.2:85979601-85979620 None:intergenic 55.0%
! GAAGTTGGTTGTGTGTGGCC+TGG - chr4.2:85979161-85979180 None:intergenic 55.0%
!! ATCACTCGAAGAGGCGCTCA+TGG + chr4.2:85980244-85980263 MS.gene33492:CDS 55.0%
AACCACCGGGCAGAGAGCTT+AGG - chr4.2:85980568-85980587 None:intergenic 60.0%
ACCACCGGGCAGAGAGCTTA+GGG - chr4.2:85980567-85980586 None:intergenic 60.0%
AGCTCTCTGCCCGGTGGTTT+AGG + chr4.2:85980569-85980588 MS.gene33492:CDS 60.0%
GACACCCTAAGCTCTCTGCC+CGG + chr4.2:85980560-85980579 MS.gene33492:CDS 60.0%
ACCCTAAGCTCTCTGCCCGG+TGG + chr4.2:85980563-85980582 MS.gene33492:CDS 65.0%
! TCTGGATCAGGCGGCGCTGA+CGG + chr4.2:85979607-85979626 MS.gene33492:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 85977904 85980624 85977904 ID=MS.gene33492
chr4.2 mRNA 85977904 85980624 85977904 ID=MS.gene33492.t1;Parent=MS.gene33492
chr4.2 exon 85977904 85977960 85977904 ID=MS.gene33492.t1.exon1;Parent=MS.gene33492.t1
chr4.2 CDS 85977904 85977960 85977904 ID=cds.MS.gene33492.t1;Parent=MS.gene33492.t1
chr4.2 exon 85978172 85978247 85978172 ID=MS.gene33492.t1.exon2;Parent=MS.gene33492.t1
chr4.2 CDS 85978172 85978247 85978172 ID=cds.MS.gene33492.t1;Parent=MS.gene33492.t1
chr4.2 exon 85978336 85978413 85978336 ID=MS.gene33492.t1.exon3;Parent=MS.gene33492.t1
chr4.2 CDS 85978336 85978413 85978336 ID=cds.MS.gene33492.t1;Parent=MS.gene33492.t1
chr4.2 exon 85978504 85978591 85978504 ID=MS.gene33492.t1.exon4;Parent=MS.gene33492.t1
chr4.2 CDS 85978504 85978591 85978504 ID=cds.MS.gene33492.t1;Parent=MS.gene33492.t1
chr4.2 exon 85979044 85979337 85979044 ID=MS.gene33492.t1.exon5;Parent=MS.gene33492.t1
chr4.2 CDS 85979044 85979337 85979044 ID=cds.MS.gene33492.t1;Parent=MS.gene33492.t1
chr4.2 exon 85979429 85979670 85979429 ID=MS.gene33492.t1.exon6;Parent=MS.gene33492.t1
chr4.2 CDS 85979429 85979670 85979429 ID=cds.MS.gene33492.t1;Parent=MS.gene33492.t1
chr4.2 exon 85979789 85979823 85979789 ID=MS.gene33492.t1.exon7;Parent=MS.gene33492.t1
chr4.2 CDS 85979789 85979823 85979789 ID=cds.MS.gene33492.t1;Parent=MS.gene33492.t1
chr4.2 exon 85979897 85979996 85979897 ID=MS.gene33492.t1.exon8;Parent=MS.gene33492.t1
chr4.2 CDS 85979897 85979996 85979897 ID=cds.MS.gene33492.t1;Parent=MS.gene33492.t1
chr4.2 exon 85980216 85980324 85980216 ID=MS.gene33492.t1.exon9;Parent=MS.gene33492.t1
chr4.2 CDS 85980216 85980324 85980216 ID=cds.MS.gene33492.t1;Parent=MS.gene33492.t1
chr4.2 exon 85980441 85980624 85980441 ID=MS.gene33492.t1.exon10;Parent=MS.gene33492.t1
chr4.2 CDS 85980441 85980624 85980441 ID=cds.MS.gene33492.t1;Parent=MS.gene33492.t1
Gene Sequence

>MS.gene33492

ATGGTAAAGGATGGAAAGAATGCAGATGTAGCTATTGATCACTATCATCGTTATAAGGTACCTCTAATGTGATATATAATTTCAGATTAATTGATACAAATTGTAATTGTAGATCAAATCATTTTAGTCCTTAAATAAATCAAATAATTTTAGTGATCAAAAGGTTATAATAGATTGACAATGTAAAAAAAGATGTATAGATTTTACTTTATGAATCTCTATTTTTTTTCTTCTAGTATATGTGTTAACCATTTTTACATCTTAATAGGAAGATGTTCAAATCATGAAGAATATGAATACAGATGCATACAGATTCTCCATATCTTGGCCAAGGATATTGCCAAGTAATAGAAAATGTTTAATTTAGTTGCATCGAAGAGTTTCAACTAGTTGTATCTTCCATATACACTGACAACTCAAACAAATATGCAGATGGAAAACTAAGTGGCGGTGTAAACCAAGCAGGAATCATATTTTACAACAACCTGATCAATGAGCTACTTGCTAATGGTAATGCATAATAATCCATACTTTATTAGTATATCATATTCATGATGTTCATATTCATAATAATACAAATTGATGGAATTATTGATCCAGGTCAAATACCATATGTGACCCTTTTTCATTGGGATCTTCCCCAGGCCTTACAAGATGAGTATGGAGGCTTTGTCAGCACGAATATTTTGTAAGTATCATGACACAAATTTGATAGAAAATGCACATACAAAATTGAGAGAATGATTAGAACGTTTTGGATACTTTAGAACGTTCTATACTGCGATTAGAATGATCTCCATACACTCACACTTTAGAGAGAAAACTACAAAGGACGATGAAACTCTTTATTATTAAATGTGATAGATATGAGAGATATGAGTGATATATATGTATGTGATTTATCAACTGAATACAAAACAAGTACTTATTATATGCTAAATTGAGTTACTTGCTCTACAAGCCACTAACCAACTAACTAACTAACCAACCAACAACATAGTTGATTTATCCACAACTAACTAACATTAGAATATGAATTATACATCAACAAAATTTTCATTCAAGAAATAATAAATTGAGGATAGAGCATTATGACATTTTTTTTATTCATATAGTTGATTGTTTAATTTCAATATGCAGAGATGATTTTAAGGACTTCGTGGATATATGCTTCAAGGAATTTGGAGACAGAGTAAAGCATTGGGTTACTTTTAATGAGCCATTCTCTTACACTTTGAATACATCAGATTGGTACCAGGCCACACACAACCAACTTCTTGCTCATGCAGCTGTTTTCGAATTGTATAAAACCACATATCAGGCTCAAAATGGTATAATAGGCATTGGATTAAACTCTCACTGGTTTAAACCATACTCAACTGACCCATTAGATCTAAAAGCTGCCGAAGACGCCCTTGATTTCATGTTTGGATGGTAACCACTATACTTTTTGACCGTTTCTACAACAACCATGGATCACGACAAAATTGGGACCGTATTGAAATATATTTTTATCTGTTTACAGGTTTATACAGCCGCTAACAACAGGAAAGTATCCAGCAAGCTTGGTGTCTTATGTGCCAGATTTACCAACGTTCACTGCAGAGCAATCTAAGAGTCTTATTGGGTCATATGATTTTATTGGAATCAATTACTACACCAGCATGTATGCTGCCAATGCAACGAAACCAATTCCTATACAGTCTGGATCAGGCGGCGCTGACGGTGTCAATAGTGTTTTCAATACTGTTAATGTGACTTTAACAGGTAAGAACACCTTTCTTACCACTCCTAATGAAGATGGCATACATCATTAATAGAAAAATCAATGTGTGCTATGATTCCTTGTTTGACATAAATTGTTTTCTGGTATGATTCATGACAGATAAGAATAAAGATGGGACATACATTGGAGCATGGGTGAGTAAGACTCTCTTGAGGTTATTGTTACATCTTATTTGATTGATGAATAACAACTAAACAATATGTGCAGGCTGCAACTTGGCTATATGTTTGTCCAAAGGGGATTCAGGACCTACTGTTGTACACAAAGGAAAAGTATAACAATCCTACGATTATCATAACAGAAAATGGTAATATTTATAATACATTCTTTTATTTTTTTTATTTACTTCTAACATAAAATTTAAGAAAATATATTATGTATATATCAATCATTTTTTATATTAAATCTACTAATTGTCATTAAAAAAAGCAAATACTTCTAATATTCTCTCATAGTGTATTTCATTCTCCTTTTTTCAGTGTCTAATAAACTAAAATTTGAAAATTCTAATAAATTTAAATCATAGGTATGAATGAAGTCAATGATCCTACACTATCACTCGAAGAGGCGCTCATGGATACTAACAGAATTGATTACTTTTATCGTCATCTATATTATCTTTTATCTGCAATGAGGTAGCACCCTCTATTTACTTAAATCAGAATCAATTATATTATTTTCGCACTCTAATTTTTTAGTTTTATGCATTTAATTTTGACTATATTGTTGTTTTTTGGTTTTTTGTGTGAAGGCAAGGTGTGAATGTAAAAGGATACTTTGCATGGTCCCTTTTGGACAACTTTGAATGGAATGACGGATACACTGTGCGTTTTGGAATTAACTTTGTTGATTATGACAATGATCTTAGAAGACACCCTAAGCTCTCTGCCCGGTGGTTTAGGAAGTTTCTTGAACATAATAGAATTATTGGTTGA
Protein sequence

>MS.gene33492.t1

MVKDGKNADVAIDHYHRYKEDVQIMKNMNTDAYRFSISWPRILPNGKLSGGVNQAGIIFYNNLINELLANGQIPYVTLFHWDLPQALQDEYGGFVSTNILDDFKDFVDICFKEFGDRVKHWVTFNEPFSYTLNTSDWYQATHNQLLAHAAVFELYKTTYQAQNGIIGIGLNSHWFKPYSTDPLDLKAAEDALDFMFGWFIQPLTTGKYPASLVSYVPDLPTFTAEQSKSLIGSYDFIGINYYTSMYAANATKPIPIQSGSGGADGVNSVFNTVNVTLTDKNKDGTYIGAWAATWLYVCPKGIQDLLLYTKEKYNNPTIIITENGMNEVNDPTLSLEEALMDTNRIDYFYRHLYYLLSAMRQGVNVKGYFAWSLLDNFEWNDGYTVRFGINFVDYDNDLRRHPKLSARWFRKFLEHNRIIG