Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33511.t1 | KEH35422.1 | 98.1 | 54 | 1 | 0 | 1 | 54 | 1 | 54 | 2.20E-19 | 104 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33511.t1 | Q6NLQ3 | 49.1 | 57 | 25 | 2 | 1 | 54 | 1 | 56 | 1.1e-06 | 53.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33511.t1 | I3SL95 | 98.1 | 54 | 1 | 0 | 1 | 54 | 1 | 54 | 1.6e-19 | 104.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene33511 | 0.800501 | 1.40E-48 | -1.69E-46 |
| MS.gene049807 | MS.gene33511 | 0.807709 | 4.43E-50 | -1.69E-46 |
| MS.gene050414 | MS.gene33511 | 0.830663 | 2.59E-55 | -1.69E-46 |
| MS.gene051201 | MS.gene33511 | 0.800022 | 1.75E-48 | -1.69E-46 |
| MS.gene052463 | MS.gene33511 | 0.820212 | 7.73E-53 | -1.69E-46 |
| MS.gene056082 | MS.gene33511 | 0.82485 | 6.45E-54 | -1.69E-46 |
| MS.gene057102 | MS.gene33511 | 0.805085 | 1.58E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene33511 | 0.802423 | 5.65E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33511.t1 | MTR_3g090530 | 98.148 | 54 | 1 | 0 | 1 | 54 | 1 | 54 | 1.31e-32 | 110 |
| MS.gene33511.t1 | MTR_3g090530 | 98.148 | 54 | 1 | 0 | 1 | 54 | 1 | 54 | 8.86e-32 | 108 |
| MS.gene33511.t1 | MTR_3g090530 | 98.148 | 54 | 1 | 0 | 1 | 54 | 1 | 54 | 1.05e-31 | 108 |
| MS.gene33511.t1 | MTR_1g028970 | 62.500 | 56 | 19 | 1 | 1 | 54 | 1 | 56 | 7.97e-17 | 69.7 |
| MS.gene33511.t1 | MTR_1g028970 | 62.500 | 56 | 19 | 1 | 1 | 54 | 1 | 56 | 2.31e-16 | 69.3 |
| MS.gene33511.t1 | MTR_1g028970 | 62.500 | 56 | 19 | 1 | 1 | 54 | 1 | 56 | 2.57e-16 | 68.9 |
| MS.gene33511.t1 | MTR_1g028960 | 64.000 | 50 | 18 | 0 | 5 | 54 | 6 | 55 | 3.42e-16 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33511.t1 | AT1G21360 | 49.123 | 57 | 25 | 2 | 1 | 54 | 1 | 56 | 2.03e-11 | 55.8 |
| MS.gene33511.t1 | AT1G21360 | 49.123 | 57 | 25 | 2 | 1 | 54 | 1 | 56 | 2.71e-11 | 55.8 |
Find 11 sgRNAs with CRISPR-Local
Find 9 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGCCTCATGATCTTCTTC+AGG | 0.264906 | 3.4:+82683756 | None:intergenic |
| TACATGGTTCTACAAGTTCT+TGG | 0.339211 | 3.4:-82683696 | None:intergenic |
| AAAGATAGGAAAGGCTTTGT+TGG | 0.395007 | 3.4:+82683723 | None:intergenic |
| GAACCATGTAACATAAAGAT+AGG | 0.421906 | 3.4:+82683709 | None:intergenic |
| AAGATAGGAAAGGCTTTGTT+GGG | 0.447613 | 3.4:+82683724 | None:intergenic |
| TTTCCTATCTTTATGTTACA+TGG | 0.447670 | 3.4:-82683712 | MS.gene33511:CDS |
| AAAGTATAAGGAAAATGAAG+AGG | 0.529810 | 3.4:-82683851 | None:intergenic |
| AAGCACTGAAAGCTCTTCAA+TGG | 0.530059 | 3.4:+82683791 | None:intergenic |
| CCTGAAGAAGATCATGAGGC+TGG | 0.568689 | 3.4:-82683756 | MS.gene33511:CDS |
| ATGTAACATAAAGATAGGAA+AGG | 0.631348 | 3.4:+82683714 | None:intergenic |
| CAAACCTGAAGAAGATCATG+AGG | 0.654696 | 3.4:-82683760 | MS.gene33511:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGTAACATAAAGATAGGAA+AGG | + | chr3.4:82683823-82683842 | None:intergenic | 25.0% |
| ! | TTTCCTATCTTTATGTTACA+TGG | - | chr3.4:82683822-82683841 | MS.gene33511:CDS | 25.0% |
| GAACCATGTAACATAAAGAT+AGG | + | chr3.4:82683828-82683847 | None:intergenic | 30.0% | |
| ! | AAAGATAGGAAAGGCTTTGT+TGG | + | chr3.4:82683814-82683833 | None:intergenic | 35.0% |
| !! | AAGATAGGAAAGGCTTTGTT+GGG | + | chr3.4:82683813-82683832 | None:intergenic | 35.0% |
| CAAACCTGAAGAAGATCATG+AGG | - | chr3.4:82683774-82683793 | MS.gene33511:CDS | 40.0% | |
| !! | AAGCACTGAAAGCTCTTCAA+TGG | + | chr3.4:82683746-82683765 | None:intergenic | 40.0% |
| CCAGCCTCATGATCTTCTTC+AGG | + | chr3.4:82683781-82683800 | None:intergenic | 50.0% | |
| CCTGAAGAAGATCATGAGGC+TGG | - | chr3.4:82683778-82683797 | MS.gene33511:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 82683697 | 82683859 | 82683697 | ID=MS.gene33511 |
| chr3.4 | mRNA | 82683697 | 82683859 | 82683697 | ID=MS.gene33511.t1;Parent=MS.gene33511 |
| chr3.4 | exon | 82683697 | 82683859 | 82683697 | ID=MS.gene33511.t1.exon1;Parent=MS.gene33511.t1 |
| chr3.4 | CDS | 82683697 | 82683859 | 82683697 | ID=cds.MS.gene33511.t1;Parent=MS.gene33511.t1 |
| Gene Sequence |
| Protein sequence |
