AlfalfaGEDB | MS.gene33512

M. sativa cultivar XinJiangDaYe / MS.gene33512

KEGG: -
GO: GO:0003674 GO:0005215 GO:0005319 GO:0005575 GO:0005737 GO:0006810 GO:0006869 GO:0008150 GO:0009987 GO:0016043 GO:0051179 GO:0051234 GO:0061024 GO:0071702 GO:0071840 GO:0110165 GO:0120009 GO:0120013
PFAM: PF08718.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33512.t1	KEH35422.1	98.3	116	2	0	1	116	1	116	1.20E-54	222.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33512.t1	Q6NLQ3	51.3	119	54	2	1	116	1	118	3.9e-25	115.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33512.t1	G7J670	98.3	116	2	0	1	116	1	116	8.8e-55	222.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050170	MS.gene33512	-0.811071	8.41E-51	-1.69E-46
MS.gene05589	MS.gene33512	-0.800081	1.70E-48	-1.69E-46
MS.gene056504	MS.gene33512	-0.800651	1.30E-48	-1.69E-46
MS.gene058334	MS.gene33512	0.809534	1.80E-50	-1.69E-46
MS.gene060595	MS.gene33512	0.812425	4.27E-51	-1.69E-46
MS.gene060982	MS.gene33512	-0.816286	5.98E-52	-1.69E-46
MS.gene061044	MS.gene33512	0.810601	1.06E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33512.t1	MTR_3g090530	98.276	116	2	0	1	116	1	116	7.40e-81	234
MS.gene33512.t1	MTR_3g090530	98.276	116	2	0	1	116	1	116	6.11e-80	234
MS.gene33512.t1	MTR_3g090530	98.276	116	2	0	1	116	1	116	8.30e-80	234
MS.gene33512.t1	MTR_1g028960	69.643	112	34	0	5	116	6	117	5.22e-53	166
MS.gene33512.t1	MTR_1g028970	66.949	118	37	1	1	116	1	118	2.36e-51	160
MS.gene33512.t1	MTR_1g028970	66.949	118	37	1	1	116	1	118	9.91e-51	160
MS.gene33512.t1	MTR_1g028970	66.949	118	37	1	1	116	1	118	1.31e-50	160
MS.gene33512.t1	MTR_3g090530	93.333	45	3	0	72	116	7	51	2.39e-24	89.7
MS.gene33512.t1	MTR_3g090530	93.333	45	3	0	72	116	7	51	5.46e-24	90.1
MS.gene33512.t1	MTR_3g090530	93.333	45	3	0	72	116	7	51	7.29e-24	89.7
MS.gene33512.t1	MTR_1g075710	35.802	81	52	0	36	116	21	101	1.76e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene33512.t1	AT1G21360	51.261	119	54	2	1	116	1	118	1.46e-37	125
MS.gene33512.t1	AT1G21360	51.261	119	54	2	1	116	1	118	2.75e-37	125
MS.gene33512.t1	AT3G21260	50.413	121	55	1	1	116	8	128	4.63e-33	115
MS.gene33512.t1	AT1G21360	50.000	56	28	0	61	116	1	56	7.80e-13	61.6

Find 25 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCCTTAACAAATTCAAATT+TGG	0.154699	3.4:+82718292	MS.gene33512:CDS
ATTTGGTTACCTTAATATTC+TGG	0.236559	3.4:-82718012	None:intergenic
CCAGCCTCATGATCTTCTTC+AGG	0.264906	3.4:-82717793	None:intergenic
AACCAAATTTGAATTTGTTA+AGG	0.324351	3.4:-82718294	None:intergenic
ACCAAATTTGAATTTGTTAA+GGG	0.329032	3.4:-82718293	None:intergenic
TACATGGTTCTACAAGTTCT+TGG	0.339211	3.4:+82717853	MS.gene33512:CDS
AGACATTCACCAGAATATTA+AGG	0.371673	3.4:+82718003	MS.gene33512:CDS
AAAGATAGGAAAGGCTTTGT+TGG	0.395007	3.4:-82717826	None:intergenic
GCGGCAGTAAATCCTTTGTT+TGG	0.397158	3.4:+82718366	MS.gene33512:CDS
GAACCATGTAACATAAAGAT+AGG	0.421906	3.4:-82717840	None:intergenic
AAGATAGGAAAGGCTTTGTT+GGG	0.447613	3.4:-82717825	None:intergenic
TTTCCTATCTTTATGTTACA+TGG	0.447670	3.4:+82717837	MS.gene33512:CDS
TGTCTCAAAACAGCCATTGT+TGG	0.448554	3.4:-82717980	None:intergenic
AATCCTTTGTTTGGCTCACT+AGG	0.459588	3.4:+82718375	MS.gene33512:CDS
AAAGTATAAGGAAAATGAAG+AGG	0.529810	3.4:+82717698	None:intergenic
AAGCACTGAAAGCTCTTCAA+TGG	0.530059	3.4:-82717758	None:intergenic
GATTGTCTTGCAGATAAGGT+AGG	0.536852	3.4:+82717956	MS.gene33512:intron
ATTGTCTTGCAGATAAGGTA+GGG	0.565172	3.4:+82717957	MS.gene33512:intron
CCTGAAGAAGATCATGAGGC+TGG	0.568689	3.4:+82717793	MS.gene33512:CDS
AATGCAAAGAAGAGAGTTAG+CGG	0.596549	3.4:+82718347	MS.gene33512:CDS
TTCAAGTCAGAAACTAGCAA+AGG	0.631092	3.4:+82718323	MS.gene33512:CDS
ATGTAACATAAAGATAGGAA+AGG	0.631348	3.4:-82717835	None:intergenic
TCACCTAGTGAGCCAAACAA+AGG	0.631414	3.4:-82718378	None:intergenic
CAAACCTGAAGAAGATCATG+AGG	0.654696	3.4:+82717789	MS.gene33512:CDS
AGATAAGGTAGGGCCAACAA+TGG	0.678454	3.4:+82717967	MS.gene33512:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAACATACTTTAAATTTGTT+TGG	-	chr3.4:82717926-82717945	None:intergenic	15.0%
!!!	AACCAAATTTGAATTTGTTA+AGG	-	chr3.4:82718297-82718316	None:intergenic	20.0%
!!!	ACCAAATTTGAATTTGTTAA+GGG	-	chr3.4:82718296-82718315	None:intergenic	20.0%
!	ACATATAGTTAGTTACACAT+TGG	+	chr3.4:82718180-82718199	MS.gene33512:intron	25.0%
!	ATGTAACATAAAGATAGGAA+AGG	-	chr3.4:82717838-82717857	None:intergenic	25.0%
!	ATTTGGTTACCTTAATATTC+TGG	-	chr3.4:82718015-82718034	None:intergenic	25.0%
!	TCAAATTATTACATGTGTGA+AGG	-	chr3.4:82718065-82718084	None:intergenic	25.0%
!	TCCCTTAACAAATTCAAATT+TGG	+	chr3.4:82718292-82718311	MS.gene33512:CDS	25.0%
!	TTTCCTATCTTTATGTTACA+TGG	+	chr3.4:82717837-82717856	MS.gene33512:CDS	25.0%
	AGACATTCACCAGAATATTA+AGG	+	chr3.4:82718003-82718022	MS.gene33512:CDS	30.0%
	GAACCATGTAACATAAAGAT+AGG	-	chr3.4:82717843-82717862	None:intergenic	30.0%
	AATGCAAAGAAGAGAGTTAG+CGG	+	chr3.4:82718347-82718366	MS.gene33512:CDS	35.0%
	ATTGTCTTGCAGATAAGGTA+GGG	+	chr3.4:82717957-82717976	MS.gene33512:intron	35.0%
	TTCAAGTCAGAAACTAGCAA+AGG	+	chr3.4:82718323-82718342	MS.gene33512:CDS	35.0%
!	AAAGATAGGAAAGGCTTTGT+TGG	-	chr3.4:82717829-82717848	None:intergenic	35.0%
!	CATTGATTGTCTTGCAGATA+AGG	+	chr3.4:82717952-82717971	MS.gene33512:intron	35.0%
!	TACATGGTTCTACAAGTTCT+TGG	+	chr3.4:82717853-82717872	MS.gene33512:CDS	35.0%
!!	AAGATAGGAAAGGCTTTGTT+GGG	-	chr3.4:82717828-82717847	None:intergenic	35.0%
	CAAACCTGAAGAAGATCATG+AGG	+	chr3.4:82717789-82717808	MS.gene33512:CDS	40.0%
	GATTGTCTTGCAGATAAGGT+AGG	+	chr3.4:82717956-82717975	MS.gene33512:intron	40.0%
	GTCATGTGTGTGTGAGTATT+TGG	-	chr3.4:82718032-82718051	None:intergenic	40.0%
	TGAGACAATATCGACACATG+TGG	-	chr3.4:82718111-82718130	None:intergenic	40.0%
	TGTCGATATTGTCTCACTGT+CGG	+	chr3.4:82718115-82718134	MS.gene33512:intron	40.0%
	TGTCTCAAAACAGCCATTGT+TGG	-	chr3.4:82717983-82718002	None:intergenic	40.0%
!	TATGTGTCATTGCATTGCAG+AGG	+	chr3.4:82718246-82718265	MS.gene33512:intron	40.0%
!!	AAGCACTGAAAGCTCTTCAA+TGG	-	chr3.4:82717761-82717780	None:intergenic	40.0%
	AGATAAGGTAGGGCCAACAA+TGG	+	chr3.4:82717967-82717986	MS.gene33512:intron	45.0%
	GCGGCAGTAAATCCTTTGTT+TGG	+	chr3.4:82718366-82718385	MS.gene33512:CDS	45.0%
	TGTCATTGCATTGCAGAGGT+TGG	+	chr3.4:82718250-82718269	MS.gene33512:intron	45.0%
	CCAGCCTCATGATCTTCTTC+AGG	-	chr3.4:82717796-82717815	None:intergenic	50.0%
	CCTGAAGAAGATCATGAGGC+TGG	+	chr3.4:82717793-82717812	MS.gene33512:CDS	50.0%
	CTCACTGTCGGACAAGAGAC+AGG	+	chr3.4:82718127-82718146	MS.gene33512:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	82717712	82718396	82717712	ID=MS.gene33512
chr3.4	mRNA	82717712	82718396	82717712	ID=MS.gene33512.t1;Parent=MS.gene33512
chr3.4	exon	82717712	82717874	82717712	ID=MS.gene33512.t1.exon1;Parent=MS.gene33512.t1
chr3.4	CDS	82717712	82717874	82717712	ID=cds.MS.gene33512.t1;Parent=MS.gene33512.t1
chr3.4	exon	82717969	82718024	82717969	ID=MS.gene33512.t1.exon2;Parent=MS.gene33512.t1
chr3.4	CDS	82717969	82718024	82717969	ID=cds.MS.gene33512.t1;Parent=MS.gene33512.t1
chr3.4	exon	82718266	82718396	82718266	ID=MS.gene33512.t1.exon3;Parent=MS.gene33512.t1
chr3.4	CDS	82718266	82718396	82718266	ID=cds.MS.gene33512.t1;Parent=MS.gene33512.t1

Gene Sequence

>MS.gene33512

ATGAAGAGGAGTAGAGATATAGAGAAGAGATCAGAGATAAACTCTGCCATTGAAGAGCTTTCAGTGCTTGTTATAGTCAAACCTGAAGAAGATCATGAGGCTGGTGCACACATCCCAACAAAGCCTTTCCTATCTTTATGTTACATGGTTCTACAAGTTCTTGGTTAGTACTAATATTTTTCTCTTTATTTTTAATCATGATTATGTAACACCAAACAAATTTAAAGTATGTTTTTTATTCATTGATTGTCTTGCAGATAAGGTAGGGCCAACAATGGCTGTTTTGAGACAAGACATTCACCAGAATATTAAGGTAACCAAATACTCACACACACATGACACTTGTGACACCTTCACACATGTAATAATTTGAGAAAATGATATAGTTGAATGTAACCACATGTGTCGATATTGTCTCACTGTCGGACAAGAGACAGGTTTTTAATTTGAAGTTACTTTTTTCAGTTAACATATAGTTAGTTACACATTGGTCAAACAAATTAACATGCAGTTATTATCAATGTGCTATTGAGTTATGTGTCATTGCATTGCAGAGGTTGGAAGCAATACATGAATCAAATCCCTTAACAAATTCAAATTTGGTTGAGATATTCAAGTCAGAAACTAGCAAAGGCAATGCAAAGAAGAGAGTTAGCGGCAGTAAATCCTTTGTTTGGCTCACTAG

Protein sequence

>MS.gene33512.t1

MKRSRDIEKRSEINSAIEELSVLVIVKPEEDHEAGAHIPTKPFLSLCYMVLQVLDKVGPTMAVLRQDIHQNIKRLEAIHESNPLTNSNLVEIFKSETSKGNAKKRVSGSKSFVWLT