Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34663.t1 | XP_013445699.1 | 92.6 | 403 | 15 | 5 | 1 | 400 | 1 | 391 | 8.10E-90 | 340.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34663.t1 | Q6ER21 | 56.9 | 274 | 71 | 2 | 67 | 340 | 41 | 267 | 1.6e-25 | 118.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34663.t1 | A0A072U1Q4 | 92.6 | 403 | 15 | 5 | 1 | 400 | 1 | 391 | 5.8e-90 | 340.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene34663.t1 | TF | zf-HD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050076 | MS.gene34663 | 0.833165 | 6.24E-56 | -1.69E-46 |
| MS.gene050581 | MS.gene34663 | 0.808779 | 2.62E-50 | -1.69E-46 |
| MS.gene050767 | MS.gene34663 | 0.80296 | 4.38E-49 | -1.69E-46 |
| MS.gene050783 | MS.gene34663 | 0.817158 | 3.81E-52 | -1.69E-46 |
| MS.gene052326 | MS.gene34663 | 0.82382 | 1.13E-53 | -1.69E-46 |
| MS.gene054376 | MS.gene34663 | 0.80174 | 7.81E-49 | -1.69E-46 |
| MS.gene054802 | MS.gene34663 | 0.829022 | 6.49E-55 | -1.69E-46 |
| MS.gene054803 | MS.gene34663 | 0.826955 | 2.04E-54 | -1.69E-46 |
| MS.gene054804 | MS.gene34663 | 0.830864 | 2.31E-55 | -1.69E-46 |
| MS.gene055277 | MS.gene34663 | 0.815651 | 8.29E-52 | -1.69E-46 |
| MS.gene055499 | MS.gene34663 | 0.804525 | 2.07E-49 | -1.69E-46 |
| MS.gene056452 | MS.gene34663 | 0.828335 | 9.52E-55 | -1.69E-46 |
| MS.gene056941 | MS.gene34663 | 0.814427 | 1.55E-51 | -1.69E-46 |
| MS.gene056942 | MS.gene34663 | 0.822316 | 2.53E-53 | -1.69E-46 |
| MS.gene058660 | MS.gene34663 | 0.806725 | 7.15E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34663.t1 | MTR_8g467340 | 89.578 | 403 | 27 | 5 | 1 | 400 | 1 | 391 | 6.85e-174 | 491 |
| MS.gene34663.t1 | MTR_7g010300 | 38.938 | 339 | 119 | 8 | 65 | 400 | 86 | 339 | 4.18e-58 | 193 |
| MS.gene34663.t1 | MTR_6g012280 | 70.968 | 62 | 18 | 0 | 66 | 127 | 90 | 151 | 9.49e-25 | 103 |
| MS.gene34663.t1 | MTR_6g012280 | 50.000 | 98 | 46 | 1 | 280 | 377 | 222 | 316 | 1.13e-24 | 103 |
| MS.gene34663.t1 | MTR_6g073013 | 62.903 | 62 | 22 | 1 | 280 | 340 | 101 | 162 | 6.42e-23 | 95.1 |
| MS.gene34663.t1 | MTR_6g073013 | 55.714 | 70 | 24 | 2 | 67 | 136 | 19 | 81 | 1.86e-18 | 82.8 |
| MS.gene34663.t1 | MTR_4g134580 | 57.353 | 68 | 29 | 0 | 280 | 347 | 284 | 351 | 1.93e-22 | 98.2 |
| MS.gene34663.t1 | MTR_4g134580 | 62.963 | 54 | 18 | 1 | 67 | 120 | 113 | 164 | 5.85e-16 | 79.0 |
| MS.gene34663.t1 | MTR_1g077020 | 60.317 | 63 | 25 | 0 | 280 | 342 | 124 | 186 | 1.06e-21 | 92.4 |
| MS.gene34663.t1 | MTR_1g077020 | 64.407 | 59 | 17 | 2 | 63 | 120 | 5 | 60 | 1.21e-20 | 89.4 |
| MS.gene34663.t1 | MTR_5g032600 | 63.492 | 63 | 23 | 0 | 280 | 342 | 209 | 271 | 2.13e-21 | 93.6 |
| MS.gene34663.t1 | MTR_5g032600 | 46.154 | 104 | 51 | 3 | 67 | 168 | 78 | 178 | 2.84e-19 | 87.4 |
| MS.gene34663.t1 | MTR_5g020510 | 56.250 | 64 | 28 | 0 | 280 | 343 | 233 | 296 | 3.83e-20 | 90.5 |
| MS.gene34663.t1 | MTR_5g020510 | 50.633 | 79 | 30 | 2 | 52 | 123 | 84 | 160 | 2.90e-19 | 87.8 |
| MS.gene34663.t1 | MTR_3g100650 | 45.783 | 83 | 45 | 0 | 260 | 342 | 191 | 273 | 1.35e-19 | 88.2 |
| MS.gene34663.t1 | MTR_3g100650 | 59.259 | 54 | 20 | 1 | 65 | 118 | 78 | 129 | 3.93e-15 | 75.5 |
| MS.gene34663.t1 | MTR_2g076780 | 46.479 | 71 | 38 | 0 | 268 | 338 | 162 | 232 | 1.74e-18 | 84.3 |
| MS.gene34663.t1 | MTR_2g076780 | 48.684 | 76 | 37 | 1 | 43 | 118 | 13 | 86 | 3.52e-16 | 77.8 |
| MS.gene34663.t1 | MTR_8g069080 | 54.839 | 62 | 28 | 0 | 277 | 338 | 202 | 263 | 1.53e-17 | 82.4 |
| MS.gene34663.t1 | MTR_8g069080 | 63.462 | 52 | 17 | 1 | 67 | 118 | 69 | 118 | 2.27e-16 | 79.0 |
| MS.gene34663.t1 | MTR_8g051480 | 48.000 | 75 | 38 | 1 | 268 | 342 | 55 | 128 | 2.39e-17 | 78.2 |
| MS.gene34663.t1 | MTR_6g012220 | 60.345 | 58 | 17 | 1 | 60 | 117 | 14 | 65 | 3.15e-17 | 76.3 |
| MS.gene34663.t1 | MTR_3g039810 | 56.667 | 60 | 24 | 1 | 65 | 124 | 18 | 75 | 4.14e-16 | 73.6 |
| MS.gene34663.t1 | MTR_8g069380 | 47.761 | 67 | 35 | 0 | 276 | 342 | 48 | 114 | 6.88e-16 | 73.9 |
| MS.gene34663.t1 | MTR_5g065440 | 55.556 | 54 | 22 | 1 | 65 | 118 | 56 | 107 | 1.28e-13 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34663.t1 | AT3G28920 | 38.400 | 375 | 126 | 11 | 1 | 374 | 7 | 277 | 3.48e-52 | 177 |
| MS.gene34663.t1 | AT1G69600 | 37.884 | 293 | 93 | 9 | 63 | 354 | 27 | 231 | 9.69e-50 | 168 |
| MS.gene34663.t1 | AT5G15210 | 72.308 | 65 | 18 | 0 | 64 | 128 | 53 | 117 | 8.31e-27 | 108 |
| MS.gene34663.t1 | AT5G15210 | 64.407 | 59 | 21 | 0 | 280 | 338 | 179 | 237 | 5.73e-22 | 95.1 |
| MS.gene34663.t1 | AT5G60480 | 65.672 | 67 | 23 | 0 | 55 | 121 | 25 | 91 | 5.42e-24 | 99.4 |
| MS.gene34663.t1 | AT5G60480 | 52.459 | 61 | 29 | 0 | 276 | 336 | 143 | 203 | 8.12e-17 | 79.3 |
| MS.gene34663.t1 | AT1G75240 | 61.905 | 63 | 24 | 0 | 277 | 339 | 237 | 299 | 2.92e-22 | 96.7 |
| MS.gene34663.t1 | AT1G75240 | 64.815 | 54 | 17 | 1 | 65 | 118 | 74 | 125 | 6.68e-17 | 81.3 |
| MS.gene34663.t1 | AT5G39760 | 77.193 | 57 | 13 | 0 | 63 | 119 | 52 | 108 | 5.30e-22 | 96.3 |
| MS.gene34663.t1 | AT1G14440 | 66.667 | 57 | 19 | 0 | 280 | 336 | 218 | 274 | 1.20e-21 | 95.1 |
| MS.gene34663.t1 | AT1G14440 | 54.545 | 66 | 28 | 1 | 64 | 129 | 87 | 150 | 1.32e-17 | 83.2 |
| MS.gene34663.t1 | AT1G14440 | 66.667 | 57 | 19 | 0 | 280 | 336 | 218 | 274 | 1.20e-21 | 95.1 |
| MS.gene34663.t1 | AT1G14440 | 54.545 | 66 | 28 | 1 | 64 | 129 | 87 | 150 | 1.32e-17 | 83.2 |
| MS.gene34663.t1 | AT4G24660 | 54.545 | 77 | 33 | 1 | 268 | 342 | 143 | 219 | 1.38e-21 | 92.8 |
| MS.gene34663.t1 | AT4G24660 | 62.500 | 56 | 19 | 1 | 65 | 120 | 47 | 100 | 1.64e-18 | 84.0 |
| MS.gene34663.t1 | AT4G24660 | 56.164 | 73 | 30 | 1 | 268 | 338 | 143 | 215 | 1.98e-21 | 92.4 |
| MS.gene34663.t1 | AT4G24660 | 62.500 | 56 | 19 | 1 | 65 | 120 | 47 | 100 | 1.32e-18 | 84.7 |
| MS.gene34663.t1 | AT2G02540 | 58.462 | 65 | 26 | 1 | 280 | 344 | 222 | 285 | 4.19e-21 | 93.2 |
| MS.gene34663.t1 | AT2G02540 | 60.345 | 58 | 21 | 1 | 63 | 120 | 83 | 138 | 6.98e-19 | 87.0 |
| MS.gene34663.t1 | AT2G02540 | 58.462 | 65 | 26 | 1 | 280 | 344 | 222 | 285 | 4.19e-21 | 93.2 |
| MS.gene34663.t1 | AT2G02540 | 60.345 | 58 | 21 | 1 | 63 | 120 | 83 | 138 | 6.98e-19 | 87.0 |
| MS.gene34663.t1 | AT2G18350 | 60.317 | 63 | 25 | 0 | 280 | 342 | 198 | 260 | 9.56e-21 | 91.3 |
| MS.gene34663.t1 | AT2G18350 | 65.385 | 52 | 16 | 1 | 67 | 118 | 82 | 131 | 3.13e-15 | 75.5 |
| MS.gene34663.t1 | AT5G65410 | 52.542 | 59 | 28 | 0 | 280 | 338 | 191 | 249 | 1.52e-18 | 85.5 |
| MS.gene34663.t1 | AT5G65410 | 62.963 | 54 | 18 | 1 | 65 | 118 | 73 | 124 | 1.97e-17 | 82.4 |
| MS.gene34663.t1 | AT1G74660 | 61.111 | 54 | 19 | 1 | 65 | 118 | 37 | 88 | 3.59e-17 | 76.6 |
| MS.gene34663.t1 | AT1G74660 | 61.111 | 54 | 19 | 1 | 65 | 118 | 37 | 88 | 7.09e-17 | 76.3 |
| MS.gene34663.t1 | AT3G50890 | 56.140 | 57 | 25 | 0 | 285 | 341 | 186 | 242 | 1.08e-16 | 79.7 |
| MS.gene34663.t1 | AT3G50890 | 40.566 | 106 | 39 | 4 | 39 | 122 | 10 | 113 | 3.79e-15 | 75.1 |
| MS.gene34663.t1 | AT3G28917 | 50.000 | 70 | 34 | 1 | 65 | 134 | 31 | 99 | 1.33e-16 | 75.5 |
| MS.gene34663.t1 | AT1G18835 | 61.111 | 54 | 16 | 1 | 65 | 118 | 24 | 72 | 1.45e-15 | 72.0 |
| MS.gene34663.t1 | AT5G42780 | 50.000 | 82 | 31 | 3 | 43 | 118 | 34 | 111 | 1.56e-13 | 70.5 |
| MS.gene34663.t1 | AT5G42780 | 45.000 | 60 | 33 | 0 | 281 | 340 | 180 | 239 | 3.34e-12 | 66.2 |
| MS.gene34663.t1 | AT1G14687 | 50.980 | 51 | 18 | 1 | 67 | 117 | 7 | 50 | 1.72e-12 | 65.9 |
Find 101 sgRNAs with CRISPR-Local
Find 117 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTAAAAGATGAAGACTTTA+AGG | 0.131907 | 8.1:+46373651 | None:intergenic |
| GATGAAGACTTTAAGGTTAT+TGG | 0.135461 | 8.1:+46373658 | None:intergenic |
| GTTCATGTTTCTTCTGTTAA+TGG | 0.168810 | 8.1:-46372554 | MS.gene34663:CDS |
| GGGCTTGTGCTTTGACTAAT+TGG | 0.181458 | 8.1:+46373208 | None:intergenic |
| GGTGATGATGGGCTTGAAAT+TGG | 0.322434 | 8.1:+46373151 | None:intergenic |
| TTAAAGTCTAATTAATTAAG+AGG | 0.354244 | 8.1:+46372526 | None:intergenic |
| CTTGGGCTTGTACTTTGACT+TGG | 0.355993 | 8.1:+46373187 | None:intergenic |
| ATTGGTGTTGTGGTAATGTT+TGG | 0.361077 | 8.1:+46373676 | None:intergenic |
| TCAGAATCTGATGTTGGAAT+TGG | 0.361684 | 8.1:-46372692 | MS.gene34663:CDS |
| GCAGCACATTTGAGGGATCT+TGG | 0.362520 | 8.1:+46373424 | None:intergenic |
| TCTGATGTTGGAATTGGAAT+TGG | 0.363182 | 8.1:-46372686 | MS.gene34663:CDS |
| TTTGAGGGATCTTGGGTCAT+TGG | 0.365376 | 8.1:+46373432 | None:intergenic |
| TTGAGGGATCTTGGGTCATT+GGG | 0.378829 | 8.1:+46373433 | None:intergenic |
| TGCTGTGGCGGTGGTGGTGG+TGG | 0.397000 | 8.1:+46373577 | None:intergenic |
| TGGTGGAGGTGATGGTTGTA+AGG | 0.406538 | 8.1:+46373353 | None:intergenic |
| TGTTGCTGTGGCGGTGGTGG+TGG | 0.411325 | 8.1:+46373574 | None:intergenic |
| TTGGGCTTGTACTTTGACTT+GGG | 0.420438 | 8.1:+46373188 | None:intergenic |
| GAGAGTGGTGGTGGTGATGA+TGG | 0.424010 | 8.1:+46373139 | None:intergenic |
| GCTCTTGTGGCTCACGGCGG+TGG | 0.424923 | 8.1:+46373380 | None:intergenic |
| GGTGGTGGTGGTTGTGGTGG+TGG | 0.428852 | 8.1:+46373253 | None:intergenic |
| CAGCACATTTGAGGGATCTT+GGG | 0.434610 | 8.1:+46373425 | None:intergenic |
| CTAGGTGGACATGCACTTGA+TGG | 0.436606 | 8.1:-46373490 | MS.gene34663:CDS |
| GGTAACGGTGGTGGTGGTTG+TGG | 0.441537 | 8.1:+46373247 | None:intergenic |
| AATAACAACAACACAAGTAG+TGG | 0.445820 | 8.1:-46372908 | MS.gene34663:CDS |
| GTGTAGATACAGCTAAGAAA+AGG | 0.451834 | 8.1:+46373295 | None:intergenic |
| GATGGGCTTGAAATTGGACT+TGG | 0.452481 | 8.1:+46373157 | None:intergenic |
| AATTGAGATTCCTTTGAAGA+TGG | 0.453296 | 8.1:+46372993 | None:intergenic |
| TTGGACTTGGGCTATGACTT+GGG | 0.456233 | 8.1:+46373170 | None:intergenic |
| TGTGGCGGTGGTGGTGGTGG+TGG | 0.457767 | 8.1:+46373580 | None:intergenic |
| GGTGGTGGAACTGTTGTTGA+AGG | 0.460734 | 8.1:+46373268 | None:intergenic |
| GGTTGTTGCTGTGGCGGTGG+TGG | 0.461889 | 8.1:+46373571 | None:intergenic |
| GATAAGAATAATGCTGCTAA+TGG | 0.462299 | 8.1:-46372635 | MS.gene34663:CDS |
| GCAAAAGAGTGATGATGGTT+TGG | 0.463952 | 8.1:-46372804 | MS.gene34663:CDS |
| ATTGGACTTGGGCTATGACT+TGG | 0.471642 | 8.1:+46373169 | None:intergenic |
| CCGCGGTGTGGCAGTGGTAA+CGG | 0.478503 | 8.1:+46373232 | None:intergenic |
| GCTGAAGAGTGATGATAAGA+AGG | 0.480903 | 8.1:+46373106 | None:intergenic |
| CTTTAAGGTTATTGGTGTTG+TGG | 0.483964 | 8.1:+46373666 | None:intergenic |
| GGCTGTTGCCGTAGTGGTGG+TGG | 0.486744 | 8.1:+46373336 | None:intergenic |
| TGGTGGTGGTGGTGTAGTAG+TGG | 0.486960 | 8.1:+46373591 | None:intergenic |
| AACGGTGGTGGTGGTTGTGG+TGG | 0.489441 | 8.1:+46373250 | None:intergenic |
| TGTGGCAGTGGTAACGGTGG+TGG | 0.492240 | 8.1:+46373238 | None:intergenic |
| TGGTGGTGTAGTAGTGGTGG+TGG | 0.494638 | 8.1:+46373597 | None:intergenic |
| GCTGGTTGTTGCTGTGGCGG+TGG | 0.495189 | 8.1:+46373568 | None:intergenic |
| CAGAAATTCTGCAATGACAT+TGG | 0.496065 | 8.1:-46372779 | MS.gene34663:CDS |
| TGGTGGTGGTTGTTGTGAGT+TGG | 0.496069 | 8.1:+46373611 | None:intergenic |
| GCCACAAGCAGCACATTTGA+GGG | 0.496943 | 8.1:+46373417 | None:intergenic |
| AGAGTGGTGGTGGTGATGAT+GGG | 0.502065 | 8.1:+46373140 | None:intergenic |
| ACAACAAGAATAGTAGTTTG+AGG | 0.503103 | 8.1:-46372721 | MS.gene34663:CDS |
| ATTGGACCGCGGTGTGGCAG+TGG | 0.507883 | 8.1:+46373226 | None:intergenic |
| TGTAGATACAGCTAAGAAAA+GGG | 0.508646 | 8.1:+46373296 | None:intergenic |
| CGTAGTGGTGGTGGAGGTGA+TGG | 0.511456 | 8.1:+46373345 | None:intergenic |
| GGCAGTGGTAACGGTGGTGG+TGG | 0.512134 | 8.1:+46373241 | None:intergenic |
| GGTGCTGGTTGTTGCTGTGG+CGG | 0.514696 | 8.1:+46373565 | None:intergenic |
| CATATGATGAATGACATTGT+TGG | 0.516395 | 8.1:+46372598 | None:intergenic |
| AAGAAATCAGAATCTGATGT+TGG | 0.519643 | 8.1:-46372698 | MS.gene34663:CDS |
| ATTCATCATATGAATGAAGA+TGG | 0.520970 | 8.1:-46372587 | MS.gene34663:CDS |
| GTGCTGCTTGTGGCTGTCAT+CGG | 0.528504 | 8.1:-46373408 | MS.gene34663:CDS |
| AGCCACAAGCAGCACATTTG+AGG | 0.529295 | 8.1:+46373416 | None:intergenic |
| AAAATGCAAAAGAGTGATGA+TGG | 0.531947 | 8.1:-46372809 | MS.gene34663:CDS |
| TCATTGGGATTGAGAGATGA+TGG | 0.533214 | 8.1:+46373448 | None:intergenic |
| GATGGTGCTGGTTGTTGCTG+TGG | 0.539760 | 8.1:+46373562 | None:intergenic |
| CATCAAGTGCATGTCCACCT+AGG | 0.550202 | 8.1:+46373491 | None:intergenic |
| ACCATCAAAACCATCTTCAA+AGG | 0.550321 | 8.1:-46373003 | MS.gene34663:CDS |
| TGGTGGTGGTGTAGTAGTGG+TGG | 0.550992 | 8.1:+46373594 | None:intergenic |
| GGCGCTGGCTGTTGCCGTAG+TGG | 0.557045 | 8.1:+46373330 | None:intergenic |
| ATCAAGTGCATGTCCACCTA+GGG | 0.558096 | 8.1:+46373492 | None:intergenic |
| TCCCTCAAATGTGCTGCTTG+TGG | 0.575497 | 8.1:-46373418 | MS.gene34663:CDS |
| GGATGTGCTCTTGTGGCTCA+CGG | 0.578300 | 8.1:+46373374 | None:intergenic |
| GGTAAATGTGAGAGTGGTGG+TGG | 0.585939 | 8.1:+46373130 | None:intergenic |
| GTTGTAAGGATGTGCTCTTG+TGG | 0.590997 | 8.1:+46373367 | None:intergenic |
| ACAACCATGGAGGATGGTGC+TGG | 0.592545 | 8.1:+46373550 | None:intergenic |
| GCTGCTAATGGTAATGATGA+TGG | 0.596655 | 8.1:-46372623 | MS.gene34663:CDS |
| GAAGAGTGATGATAAGAAGG+TGG | 0.597173 | 8.1:+46373109 | None:intergenic |
| ATGGGCTTGAAATTGGACTT+GGG | 0.599703 | 8.1:+46373158 | None:intergenic |
| CATCACCTCCACCACCACTA+CGG | 0.605016 | 8.1:-46373344 | MS.gene34663:CDS |
| TAAGAAACAACCATGGAGGA+TGG | 0.606050 | 8.1:+46373544 | None:intergenic |
| AATGACATTGGTGTTTCTAG+AGG | 0.607705 | 8.1:-46372767 | MS.gene34663:CDS |
| TGTTGCCGTAGTGGTGGTGG+AGG | 0.609429 | 8.1:+46373339 | None:intergenic |
| CCAAGAGCTAAAAGCATGTG+AGG | 0.612524 | 8.1:+46373082 | None:intergenic |
| ACAACCAGCACCATCCTCCA+TGG | 0.616140 | 8.1:-46373554 | MS.gene34663:CDS |
| ATAACAACAACACAAGTAGT+GGG | 0.619615 | 8.1:-46372907 | MS.gene34663:CDS |
| GAAGGTGGTAAATGTGAGAG+TGG | 0.625404 | 8.1:+46373124 | None:intergenic |
| GGACATGCACTTGATGGATG+TGG | 0.627027 | 8.1:-46373484 | MS.gene34663:CDS |
| GAGCAGAAAGAGAAGATGCA+TGG | 0.627308 | 8.1:-46372854 | MS.gene34663:CDS |
| TAGATACAGCTAAGAAAAGG+GGG | 0.630616 | 8.1:+46373298 | None:intergenic |
| TGATCATGATCAGAGTGTTG+TGG | 0.633373 | 8.1:+46373037 | None:intergenic |
| TGACTAATTGGACCGCGGTG+TGG | 0.633668 | 8.1:+46373220 | None:intergenic |
| GCTGGCTGTTGCCGTAGTGG+TGG | 0.640850 | 8.1:+46373333 | None:intergenic |
| TGCTTTGACTAATTGGACCG+CGG | 0.655033 | 8.1:+46373215 | None:intergenic |
| TCATGATCAGAGTGTTGTGG+TGG | 0.665411 | 8.1:+46373040 | None:intergenic |
| GTAGATACAGCTAAGAAAAG+GGG | 0.668570 | 8.1:+46373297 | None:intergenic |
| TTCTTTGTAAGAAACAACCA+TGG | 0.679590 | 8.1:+46373537 | None:intergenic |
| GTACAGGACAAAATTCAGCA+AGG | 0.690921 | 8.1:-46372876 | MS.gene34663:CDS |
| GGTGGTAAATGTGAGAGTGG+TGG | 0.697780 | 8.1:+46373127 | None:intergenic |
| AATCATGCTGCATCCCTAGG+TGG | 0.699222 | 8.1:-46373505 | MS.gene34663:CDS |
| TTTGTAAGAAACAACCATGG+AGG | 0.701159 | 8.1:+46373540 | None:intergenic |
| CGGTGTGGCAGTGGTAACGG+TGG | 0.709392 | 8.1:+46373235 | None:intergenic |
| TGTGCTCTTGTGGCTCACGG+CGG | 0.720758 | 8.1:+46373377 | None:intergenic |
| AGATACAGCTAAGAAAAGGG+GGG | 0.740250 | 8.1:+46373299 | None:intergenic |
| CCGTTACCACTGCCACACCG+CGG | 0.766284 | 8.1:-46373232 | MS.gene34663:CDS |
| TTGGGATTGAGAGATGATGG+TGG | 0.770102 | 8.1:+46373451 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTCATCATATGAATGAAGA+TGG | - | chr8.1:46373675-46373694 | MS.gene34663:CDS | 25.0% |
| ! | TGTAAAAGATGAAGACTTTA+AGG | + | chr8.1:46372614-46372633 | None:intergenic | 25.0% |
| !! | AAGTCTTCATCTTTTACAAA+CGG | - | chr8.1:46372616-46372635 | MS.gene34663:CDS | 25.0% |
| AAAATGCAAAAGAGTGATGA+TGG | - | chr8.1:46373453-46373472 | MS.gene34663:CDS | 30.0% | |
| AAGAAATCAGAATCTGATGT+TGG | - | chr8.1:46373564-46373583 | MS.gene34663:CDS | 30.0% | |
| AATAACAACAACACAAGTAG+TGG | - | chr8.1:46373354-46373373 | MS.gene34663:CDS | 30.0% | |
| ACAACAAGAATAGTAGTTTG+AGG | - | chr8.1:46373541-46373560 | MS.gene34663:CDS | 30.0% | |
| ATAACAACAACACAAGTAGT+GGG | - | chr8.1:46373355-46373374 | MS.gene34663:CDS | 30.0% | |
| CATATGATGAATGACATTGT+TGG | + | chr8.1:46373667-46373686 | None:intergenic | 30.0% | |
| GATAAGAATAATGCTGCTAA+TGG | - | chr8.1:46373627-46373646 | MS.gene34663:CDS | 30.0% | |
| GTTCATGTTTCTTCTGTTAA+TGG | - | chr8.1:46373708-46373727 | MS.gene34663:CDS | 30.0% | |
| TGTAGATACAGCTAAGAAAA+GGG | + | chr8.1:46372969-46372988 | None:intergenic | 30.0% | |
| TTCTTTGTAAGAAACAACCA+TGG | + | chr8.1:46372728-46372747 | None:intergenic | 30.0% | |
| ! | AATTGAGATTCCTTTGAAGA+TGG | + | chr8.1:46373272-46373291 | None:intergenic | 30.0% |
| !! | CATGGTTTTTCTGAGAAATT+GGG | - | chr8.1:46373426-46373445 | MS.gene34663:CDS | 30.0% |
| !! | GATGAAGACTTTAAGGTTAT+TGG | + | chr8.1:46372607-46372626 | None:intergenic | 30.0% |
| ACCATCAAAACCATCTTCAA+AGG | - | chr8.1:46373259-46373278 | MS.gene34663:CDS | 35.0% | |
| CAGAAATTCTGCAATGACAT+TGG | - | chr8.1:46373483-46373502 | MS.gene34663:CDS | 35.0% | |
| GTAGATACAGCTAAGAAAAG+GGG | + | chr8.1:46372968-46372987 | None:intergenic | 35.0% | |
| GTGTAGATACAGCTAAGAAA+AGG | + | chr8.1:46372970-46372989 | None:intergenic | 35.0% | |
| TAGATACAGCTAAGAAAAGG+GGG | + | chr8.1:46372967-46372986 | None:intergenic | 35.0% | |
| TCAGAATCTGATGTTGGAAT+TGG | - | chr8.1:46373570-46373589 | MS.gene34663:CDS | 35.0% | |
| TCTGATGTTGGAATTGGAAT+TGG | - | chr8.1:46373576-46373595 | MS.gene34663:CDS | 35.0% | |
| TTTGTAAGAAACAACCATGG+AGG | + | chr8.1:46372725-46372744 | None:intergenic | 35.0% | |
| ! | CTAGAGGAGTTTTCAAAGTT+TGG | - | chr8.1:46373511-46373530 | MS.gene34663:CDS | 35.0% |
| ! | TGTTGTTGTTGTTGTTGTTG+TGG | + | chr8.1:46373323-46373342 | None:intergenic | 35.0% |
| !! | AATGACATTGGTGTTTCTAG+AGG | - | chr8.1:46373495-46373514 | MS.gene34663:CDS | 35.0% |
| !! | ATTGGTGTTGTGGTAATGTT+TGG | + | chr8.1:46372589-46372608 | None:intergenic | 35.0% |
| !! | CTTTAAGGTTATTGGTGTTG+TGG | + | chr8.1:46372599-46372618 | None:intergenic | 35.0% |
| !! | GCATGGTTTTTCTGAGAAAT+TGG | - | chr8.1:46373425-46373444 | MS.gene34663:CDS | 35.0% |
| !! | GTTTTTCTGAGAAATTGGGT+TGG | - | chr8.1:46373430-46373449 | MS.gene34663:CDS | 35.0% |
| !!! | TCCTTTGAAGATGGTTTTGA+TGG | + | chr8.1:46373263-46373282 | None:intergenic | 35.0% |
| !!! | TTTGAAGATGGTTTTGATGG+TGG | + | chr8.1:46373260-46373279 | None:intergenic | 35.0% |
| AAAAATCATGCTGCATCCCT+AGG | - | chr8.1:46372754-46372773 | MS.gene34663:CDS | 40.0% | |
| AGATACAGCTAAGAAAAGGG+GGG | + | chr8.1:46372966-46372985 | None:intergenic | 40.0% | |
| ATGGGCTTGAAATTGGACTT+GGG | + | chr8.1:46373107-46373126 | None:intergenic | 40.0% | |
| GAAGAGTGATGATAAGAAGG+TGG | + | chr8.1:46373156-46373175 | None:intergenic | 40.0% | |
| GCTGAAGAGTGATGATAAGA+AGG | + | chr8.1:46373159-46373178 | None:intergenic | 40.0% | |
| GTACAGGACAAAATTCAGCA+AGG | - | chr8.1:46373386-46373405 | MS.gene34663:CDS | 40.0% | |
| TAAGAAACAACCATGGAGGA+TGG | + | chr8.1:46372721-46372740 | None:intergenic | 40.0% | |
| TCATTGGGATTGAGAGATGA+TGG | + | chr8.1:46372817-46372836 | None:intergenic | 40.0% | |
| ! | ACACAAGTAGTGGGAAAAAG+AGG | - | chr8.1:46373364-46373383 | MS.gene34663:CDS | 40.0% |
| ! | GCTGCTAATGGTAATGATGA+TGG | - | chr8.1:46373639-46373658 | MS.gene34663:CDS | 40.0% |
| ! | TTGGGCTTGTACTTTGACTT+GGG | + | chr8.1:46373077-46373096 | None:intergenic | 40.0% |
| !! | GCAAAAGAGTGATGATGGTT+TGG | - | chr8.1:46373458-46373477 | MS.gene34663:CDS | 40.0% |
| !! | TGATCATGATCAGAGTGTTG+TGG | + | chr8.1:46373228-46373247 | None:intergenic | 40.0% |
| ATCAAGTGCATGTCCACCTA+GGG | + | chr8.1:46372773-46372792 | None:intergenic | 45.0% | |
| ATTGGACTTGGGCTATGACT+TGG | + | chr8.1:46373096-46373115 | None:intergenic | 45.0% | |
| CAGCACATTTGAGGGATCTT+GGG | + | chr8.1:46372840-46372859 | None:intergenic | 45.0% | |
| CTTGGGCTTGTACTTTGACT+TGG | + | chr8.1:46373078-46373097 | None:intergenic | 45.0% | |
| GAAGGTGGTAAATGTGAGAG+TGG | + | chr8.1:46373141-46373160 | None:intergenic | 45.0% | |
| GAGCAGAAAGAGAAGATGCA+TGG | - | chr8.1:46373408-46373427 | MS.gene34663:CDS | 45.0% | |
| GGGCTTGTGCTTTGACTAAT+TGG | + | chr8.1:46373057-46373076 | None:intergenic | 45.0% | |
| GTTGTAAGGATGTGCTCTTG+TGG | + | chr8.1:46372898-46372917 | None:intergenic | 45.0% | |
| TGCTTTGACTAATTGGACCG+CGG | + | chr8.1:46373050-46373069 | None:intergenic | 45.0% | |
| TTGAGGGATCTTGGGTCATT+GGG | + | chr8.1:46372832-46372851 | None:intergenic | 45.0% | |
| TTGGACTTGGGCTATGACTT+GGG | + | chr8.1:46373095-46373114 | None:intergenic | 45.0% | |
| TTTGAGGGATCTTGGGTCAT+TGG | + | chr8.1:46372833-46372852 | None:intergenic | 45.0% | |
| ! | CCAAGAGCTAAAAGCATGTG+AGG | + | chr8.1:46373183-46373202 | None:intergenic | 45.0% |
| ! | GATGGGCTTGAAATTGGACT+TGG | + | chr8.1:46373108-46373127 | None:intergenic | 45.0% |
| ! | GTAGTGGGAAAAAGAGGTAC+AGG | - | chr8.1:46373370-46373389 | MS.gene34663:CDS | 45.0% |
| ! | TTGGGATTGAGAGATGATGG+TGG | + | chr8.1:46372814-46372833 | None:intergenic | 45.0% |
| !! | AAGAAAAGGGGGGCTGTTTT+TGG | + | chr8.1:46372956-46372975 | None:intergenic | 45.0% |
| !! | GGTGATGATGGGCTTGAAAT+TGG | + | chr8.1:46373114-46373133 | None:intergenic | 45.0% |
| !! | TCATGATCAGAGTGTTGTGG+TGG | + | chr8.1:46373225-46373244 | None:intergenic | 45.0% |
| !!! | CACATGCTTTTAGCTCTTGG+TGG | - | chr8.1:46373183-46373202 | MS.gene34663:CDS | 45.0% |
| !!! | CCTCACATGCTTTTAGCTCT+TGG | - | chr8.1:46373180-46373199 | MS.gene34663:CDS | 45.0% |
| !!! | GAAGATGGTTTTGATGGTGG+TGG | + | chr8.1:46373257-46373276 | None:intergenic | 45.0% |
| AATCATGCTGCATCCCTAGG+TGG | - | chr8.1:46372757-46372776 | MS.gene34663:CDS | 50.0% | |
| AGCCACAAGCAGCACATTTG+AGG | + | chr8.1:46372849-46372868 | None:intergenic | 50.0% | |
| CATCAAGTGCATGTCCACCT+AGG | + | chr8.1:46372774-46372793 | None:intergenic | 50.0% | |
| CTAGGTGGACATGCACTTGA+TGG | - | chr8.1:46372772-46372791 | MS.gene34663:CDS | 50.0% | |
| GCAGCACATTTGAGGGATCT+TGG | + | chr8.1:46372841-46372860 | None:intergenic | 50.0% | |
| GCCACAAGCAGCACATTTGA+GGG | + | chr8.1:46372848-46372867 | None:intergenic | 50.0% | |
| GGTAAATGTGAGAGTGGTGG+TGG | + | chr8.1:46373135-46373154 | None:intergenic | 50.0% | |
| GGTGGTAAATGTGAGAGTGG+TGG | + | chr8.1:46373138-46373157 | None:intergenic | 50.0% | |
| ! | GGACATGCACTTGATGGATG+TGG | - | chr8.1:46372778-46372797 | MS.gene34663:CDS | 50.0% |
| ! | TGGTGGAGGTGATGGTTGTA+AGG | + | chr8.1:46372912-46372931 | None:intergenic | 50.0% |
| !! | AGAGTGGTGGTGGTGATGAT+GGG | + | chr8.1:46373125-46373144 | None:intergenic | 50.0% |
| !! | GGTGGTGGAACTGTTGTTGA+AGG | + | chr8.1:46372997-46373016 | None:intergenic | 50.0% |
| !! | TCCCTCAAATGTGCTGCTTG+TGG | - | chr8.1:46372844-46372863 | MS.gene34663:CDS | 50.0% |
| !! | TGGTGGTGGTTGTTGTGAGT+TGG | + | chr8.1:46372654-46372673 | None:intergenic | 50.0% |
| !!! | GATGGTTTTGATGGTGGTGG+TGG | + | chr8.1:46373254-46373273 | None:intergenic | 50.0% |
| ACAACCAGCACCATCCTCCA+TGG | - | chr8.1:46372708-46372727 | MS.gene34663:CDS | 55.0% | |
| ACAACCATGGAGGATGGTGC+TGG | + | chr8.1:46372715-46372734 | None:intergenic | 55.0% | |
| CATCACCTCCACCACCACTA+CGG | - | chr8.1:46372918-46372937 | MS.gene34663:CDS | 55.0% | |
| GGATGTGCTCTTGTGGCTCA+CGG | + | chr8.1:46372891-46372910 | None:intergenic | 55.0% | |
| TGACTAATTGGACCGCGGTG+TGG | + | chr8.1:46373045-46373064 | None:intergenic | 55.0% | |
| ! | TGGTGGTGTAGTAGTGGTGG+TGG | + | chr8.1:46372668-46372687 | None:intergenic | 55.0% |
| !! | GAGAGTGGTGGTGGTGATGA+TGG | + | chr8.1:46373126-46373145 | None:intergenic | 55.0% |
| !! | GATGGTGCTGGTTGTTGCTG+TGG | + | chr8.1:46372703-46372722 | None:intergenic | 55.0% |
| !! | GTGCTGCTTGTGGCTGTCAT+CGG | - | chr8.1:46372854-46372873 | MS.gene34663:CDS | 55.0% |
| !! | TGGTGGTGGTGGTGTAGTAG+TGG | + | chr8.1:46372674-46372693 | None:intergenic | 55.0% |
| !! | TGGTGGTGGTGTAGTAGTGG+TGG | + | chr8.1:46372671-46372690 | None:intergenic | 55.0% |
| TGTGCTCTTGTGGCTCACGG+CGG | + | chr8.1:46372888-46372907 | None:intergenic | 60.0% | |
| !! | AACGGTGGTGGTGGTTGTGG+TGG | + | chr8.1:46373015-46373034 | None:intergenic | 60.0% |
| !! | CGTAGTGGTGGTGGAGGTGA+TGG | + | chr8.1:46372920-46372939 | None:intergenic | 60.0% |
| !! | GGTAACGGTGGTGGTGGTTG+TGG | + | chr8.1:46373018-46373037 | None:intergenic | 60.0% |
| !! | GGTGCTGGTTGTTGCTGTGG+CGG | + | chr8.1:46372700-46372719 | None:intergenic | 60.0% |
| !! | TGTGGCAGTGGTAACGGTGG+TGG | + | chr8.1:46373027-46373046 | None:intergenic | 60.0% |
| !! | TGTTGCCGTAGTGGTGGTGG+AGG | + | chr8.1:46372926-46372945 | None:intergenic | 60.0% |
| ATTGGACCGCGGTGTGGCAG+TGG | + | chr8.1:46373039-46373058 | None:intergenic | 65.0% | |
| CCGCGGTGTGGCAGTGGTAA+CGG | + | chr8.1:46373033-46373052 | None:intergenic | 65.0% | |
| CCGTTACCACTGCCACACCG+CGG | - | chr8.1:46373030-46373049 | MS.gene34663:CDS | 65.0% | |
| GCTGGCTGTTGCCGTAGTGG+TGG | + | chr8.1:46372932-46372951 | None:intergenic | 65.0% | |
| GCTGGTTGTTGCTGTGGCGG+TGG | + | chr8.1:46372697-46372716 | None:intergenic | 65.0% | |
| GGCTGTTGCCGTAGTGGTGG+TGG | + | chr8.1:46372929-46372948 | None:intergenic | 65.0% | |
| GGTTGTTGCTGTGGCGGTGG+TGG | + | chr8.1:46372694-46372713 | None:intergenic | 65.0% | |
| ! | TGTTGCTGTGGCGGTGGTGG+TGG | + | chr8.1:46372691-46372710 | None:intergenic | 65.0% |
| !! | CGGTGTGGCAGTGGTAACGG+TGG | + | chr8.1:46373030-46373049 | None:intergenic | 65.0% |
| !! | GGCAGTGGTAACGGTGGTGG+TGG | + | chr8.1:46373024-46373043 | None:intergenic | 65.0% |
| !! | GGTGGTGGTGGTTGTGGTGG+TGG | + | chr8.1:46373012-46373031 | None:intergenic | 65.0% |
| !!! | AGGGGGGCTGTTTTTGGCGC+TGG | + | chr8.1:46372950-46372969 | None:intergenic | 65.0% |
| GCTCTTGTGGCTCACGGCGG+TGG | + | chr8.1:46372885-46372904 | None:intergenic | 70.0% | |
| !! | GGCGCTGGCTGTTGCCGTAG+TGG | + | chr8.1:46372935-46372954 | None:intergenic | 70.0% |
| !! | TGCTGTGGCGGTGGTGGTGG+TGG | + | chr8.1:46372688-46372707 | None:intergenic | 70.0% |
| !! | TGTGGCGGTGGTGGTGGTGG+TGG | + | chr8.1:46372685-46372704 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 46372541 | 46373743 | 46372541 | ID=MS.gene34663 |
| chr8.1 | mRNA | 46372541 | 46373743 | 46372541 | ID=MS.gene34663.t1;Parent=MS.gene34663 |
| chr8.1 | exon | 46372541 | 46373743 | 46372541 | ID=MS.gene34663.t1.exon1;Parent=MS.gene34663.t1 |
| chr8.1 | CDS | 46372541 | 46373743 | 46372541 | ID=cds.MS.gene34663.t1;Parent=MS.gene34663.t1 |
| Gene Sequence |
| Protein sequence |
