Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34976.t1 | RHN42315.1 | 100 | 115 | 0 | 0 | 20 | 134 | 50 | 164 | 1.40E-57 | 232.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34976.t1 | A0A396GU48 | 100.0 | 115 | 0 | 0 | 20 | 134 | 50 | 164 | 9.8e-58 | 232.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049298 | MS.gene34976 | 0.811826 | 5.76E-51 | -1.69E-46 |
| MS.gene050707 | MS.gene34976 | 0.825428 | 4.71E-54 | -1.69E-46 |
| MS.gene052209 | MS.gene34976 | 0.817244 | 3.65E-52 | -1.69E-46 |
| MS.gene05652 | MS.gene34976 | 0.80354 | 3.32E-49 | -1.69E-46 |
| MS.gene057669 | MS.gene34976 | 0.812668 | 3.78E-51 | -1.69E-46 |
| MS.gene058128 | MS.gene34976 | 0.827757 | 1.31E-54 | -1.69E-46 |
| MS.gene058853 | MS.gene34976 | 0.828134 | 1.06E-54 | -1.69E-46 |
| MS.gene059616 | MS.gene34976 | 0.804484 | 2.11E-49 | -1.69E-46 |
| MS.gene059905 | MS.gene34976 | 0.823774 | 1.16E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34976.t1 | MTR_5g027940 | 36.842 | 76 | 47 | 1 | 15 | 90 | 50 | 124 | 9.67e-14 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34976.t1 | AT1G31940 | 43.396 | 53 | 30 | 0 | 15 | 67 | 49 | 101 | 2.95e-12 | 60.5 |
| MS.gene34976.t1 | AT2G35585 | 41.818 | 55 | 31 | 1 | 15 | 68 | 48 | 102 | 4.36e-11 | 57.4 |
Find 42 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGGAAAATAGTGATATTC+TGG | 0.208224 | 4.4:+18007908 | None:intergenic |
| CGCTTTCGCACTCCTCGAGC+TGG | 0.260475 | 4.4:+18008015 | None:intergenic |
| GGCTCCATAGGATGTTCAAC+TGG | 0.393061 | 4.4:+18008884 | None:intergenic |
| AGGAAGAGAGGTGAGATGTC+TGG | 0.414508 | 4.4:-18008075 | MS.gene34976:CDS |
| GCTTTCGCACTCCTCGAGCT+GGG | 0.417036 | 4.4:+18008016 | None:intergenic |
| TTTCCCAGTTGAACATCCTA+TGG | 0.422933 | 4.4:-18008888 | MS.gene34976:CDS |
| ACACTAATGACTGAAGATTC+AGG | 0.437311 | 4.4:+18008827 | None:intergenic |
| ACCCTCACTCATGGATCATC+AGG | 0.442814 | 4.4:-18007985 | MS.gene34976:CDS |
| TTGGAAAATAGTGATATTCT+GGG | 0.444826 | 4.4:+18007909 | None:intergenic |
| TTTCCAAGTGCTTCAGCAGT+TGG | 0.454811 | 4.4:-18007893 | MS.gene34976:CDS |
| AGAGGTGAGATGTCTGGGTC+TGG | 0.464003 | 4.4:-18008069 | MS.gene34976:CDS |
| AGAGGAACACAGAAAGCATA+AGG | 0.478521 | 4.4:-18008095 | MS.gene34976:CDS |
| TGTCCAACTGCTGAAGCACT+TGG | 0.496596 | 4.4:+18007890 | None:intergenic |
| TCTTCATCCGGTGGCTCCAT+AGG | 0.504589 | 4.4:+18008872 | None:intergenic |
| GGGTCTGGACAAAGAACAGC+TGG | 0.506189 | 4.4:-18008054 | MS.gene34976:CDS |
| AGATGTGGCATTCTCATCAA+TGG | 0.507933 | 4.4:+18007946 | None:intergenic |
| ATATTCTGGGATTGTAGAGG+AGG | 0.508528 | 4.4:+18007922 | None:intergenic |
| CGGCATCACACCCTCACTCA+TGG | 0.508751 | 4.4:-18007994 | MS.gene34976:CDS |
| ATGACTGAAGATTCAGGCAT+TGG | 0.525006 | 4.4:+18008833 | None:intergenic |
| GCCACCGGATGAAGATCGTC+CGG | 0.527419 | 4.4:-18008864 | MS.gene34976:CDS |
| GGAAGAGAGGTGAGATGTCT+GGG | 0.529996 | 4.4:-18008074 | MS.gene34976:CDS |
| CAGCTCGAGGAGTGCGAAAG+CGG | 0.537056 | 4.4:-18008014 | MS.gene34976:CDS |
| TGGACAAAGAACAGCTGGCA+TGG | 0.537925 | 4.4:-18008049 | MS.gene34976:CDS |
| ATGAAAGGATGCATGAAAAG+AGG | 0.540753 | 4.4:-18008113 | MS.gene34976:CDS |
| TCAGGCATTGGACATTTCAC+CGG | 0.545910 | 4.4:+18008845 | None:intergenic |
| TATTCTGGGATTGTAGAGGA+GGG | 0.548828 | 4.4:+18007923 | None:intergenic |
| TTCACCGGACGATCTTCATC+CGG | 0.561909 | 4.4:+18008860 | None:intergenic |
| CTTTCGCACTCCTCGAGCTG+GGG | 0.565176 | 4.4:+18008017 | None:intergenic |
| TGAACATCCTATGGAGCCAC+CGG | 0.581194 | 4.4:-18008879 | MS.gene34976:CDS |
| ACAGAAAGCATAAGGAAGAG+AGG | 0.581626 | 4.4:-18008087 | MS.gene34976:CDS |
| CAACCCTCTGATGATGATGA+TGG | 0.596745 | 4.4:+18008927 | None:intergenic |
| TCCTGATGATCCATGAGTGA+GGG | 0.599861 | 4.4:+18007984 | None:intergenic |
| GACACAGATGCCCCAGCTCG+AGG | 0.604247 | 4.4:-18008027 | MS.gene34976:CDS |
| ACCGGACGATCTTCATCCGG+TGG | 0.608873 | 4.4:+18008863 | None:intergenic |
| GATTGTAGAGGAGGGAGATG+TGG | 0.615192 | 4.4:+18007931 | None:intergenic |
| GTGATATTCTGGGATTGTAG+AGG | 0.620846 | 4.4:+18007919 | None:intergenic |
| TCTGATGATGATGATGGTGG+TGG | 0.634001 | 4.4:+18008933 | None:intergenic |
| GCTCCATAGGATGTTCAACT+GGG | 0.634698 | 4.4:+18008885 | None:intergenic |
| ACCACCATCATCATCATCAG+AGG | 0.640128 | 4.4:-18008931 | MS.gene34976:CDS |
| CCACCATCATCATCATCAGA+GGG | 0.663413 | 4.4:-18008930 | MS.gene34976:CDS |
| CTCCTGATGATCCATGAGTG+AGG | 0.684933 | 4.4:+18007983 | None:intergenic |
| CCCTCTGATGATGATGATGG+TGG | 0.698156 | 4.4:+18008930 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACTAATAATAAAACTAAA+AGG | - | chr4.4:18008266-18008285 | MS.gene34976:intron | 10.0% |
| !! | AACAATTATTGACAAATGTT+TGG | - | chr4.4:18008518-18008537 | MS.gene34976:intron | 20.0% |
| !! | ATAAAGCATGAAGATAAAAA+TGG | + | chr4.4:18008579-18008598 | None:intergenic | 20.0% |
| !! | CAAAATGTCTAATGAATATT+TGG | + | chr4.4:18008385-18008404 | None:intergenic | 20.0% |
| !!! | CAAATATTCATTAGACATTT+TGG | - | chr4.4:18008383-18008402 | MS.gene34976:intron | 20.0% |
| !!! | TTATTTCACTAAAACTTGTT+TGG | - | chr4.4:18008595-18008614 | MS.gene34976:intron | 20.0% |
| ! | AAGGAAATCAATATAGTTCT+AGG | + | chr4.4:18008477-18008496 | None:intergenic | 25.0% |
| ! | GACACATTATTATATGAACA+CGG | + | chr4.4:18008183-18008202 | None:intergenic | 25.0% |
| ! | TATTTGGATAGATATATCAC+AGG | + | chr4.4:18008369-18008388 | None:intergenic | 25.0% |
| ! | TTGGAAAATAGTGATATTCT+GGG | + | chr4.4:18008920-18008939 | None:intergenic | 25.0% |
| !! | AAAACTTGTTTGGATTCTTT+AGG | - | chr4.4:18008605-18008624 | MS.gene34976:intron | 25.0% |
| !!! | TAGAACTATATTGATTTCCT+TGG | - | chr4.4:18008476-18008495 | MS.gene34976:intron | 25.0% |
| AATGTTTGGCAACAATTTAC+CGG | - | chr4.4:18008532-18008551 | MS.gene34976:intron | 30.0% | |
| CTTGGAAAATAGTGATATTC+TGG | + | chr4.4:18008921-18008940 | None:intergenic | 30.0% | |
| GTTACCTACTTATCTTAAGA+TGG | - | chr4.4:18008045-18008064 | MS.gene34976:CDS | 30.0% | |
| TTTGCCATCTTAAGATAAGT+AGG | + | chr4.4:18008052-18008071 | None:intergenic | 30.0% | |
| ! | ATAATGTGTCACTAAACACT+AGG | - | chr4.4:18008193-18008212 | MS.gene34976:intron | 30.0% |
| ! | GACTAGAATCAGCTTTTAAA+TGG | - | chr4.4:18008288-18008307 | MS.gene34976:intron | 30.0% |
| ! | TCATTAGACATTTTGGACTA+GGG | - | chr4.4:18008390-18008409 | MS.gene34976:intron | 30.0% |
| ! | TTCATTAGACATTTTGGACT+AGG | - | chr4.4:18008389-18008408 | MS.gene34976:intron | 30.0% |
| ACACTAATGACTGAAGATTC+AGG | + | chr4.4:18008002-18008021 | None:intergenic | 35.0% | |
| ACATTTAACAACTGCTACCA+AGG | + | chr4.4:18008496-18008515 | None:intergenic | 35.0% | |
| ACCAATTTGGAAATTTCAGG+TGG | - | chr4.4:18008223-18008242 | MS.gene34976:intron | 35.0% | |
| ACCACCTGAAATTTCCAAAT+TGG | + | chr4.4:18008227-18008246 | None:intergenic | 35.0% | |
| ATGAAAGGATGCATGAAAAG+AGG | - | chr4.4:18008713-18008732 | MS.gene34976:intron | 35.0% | |
| ATTATATGAACACGGCTACA+AGG | + | chr4.4:18008175-18008194 | None:intergenic | 35.0% | |
| ! | CATGACTTTTACAGGATGAA+AGG | - | chr4.4:18008698-18008717 | MS.gene34976:intron | 35.0% |
| !! | CATTAGACATTTTGGACTAG+GGG | - | chr4.4:18008391-18008410 | MS.gene34976:intron | 35.0% |
| AGATGTGGCATTCTCATCAA+TGG | + | chr4.4:18008883-18008902 | None:intergenic | 40.0% | |
| ATGACTGAAGATTCAGGCAT+TGG | + | chr4.4:18007996-18008015 | None:intergenic | 40.0% | |
| CAACAATTTACCGGTCATTC+TGG | - | chr4.4:18008541-18008560 | MS.gene34976:intron | 40.0% | |
| CTAGAAAGAGAAGCTGCAAA+GGG | + | chr4.4:18008419-18008438 | None:intergenic | 40.0% | |
| GGGACCAATTTGGAAATTTC+AGG | - | chr4.4:18008220-18008239 | MS.gene34976:intron | 40.0% | |
| GTCACTAAACACTAGGCTAA+GGG | - | chr4.4:18008200-18008219 | MS.gene34976:intron | 40.0% | |
| TCAGTAGTTTCCAGAATGAC+CGG | + | chr4.4:18008554-18008573 | None:intergenic | 40.0% | |
| TGTCACTAAACACTAGGCTA+AGG | - | chr4.4:18008199-18008218 | MS.gene34976:intron | 40.0% | |
| TTTCCCAGTTGAACATCCTA+TGG | - | chr4.4:18007938-18007957 | MS.gene34976:CDS | 40.0% | |
| ! | ACAGAAAGCATAAGGAAGAG+AGG | - | chr4.4:18008739-18008758 | MS.gene34976:intron | 40.0% |
| ! | AGAGGAACACAGAAAGCATA+AGG | - | chr4.4:18008731-18008750 | MS.gene34976:intron | 40.0% |
| ! | CTGACAAGCATGACTTTTAC+AGG | - | chr4.4:18008690-18008709 | MS.gene34976:intron | 40.0% |
| !! | ATATTCTGGGATTGTAGAGG+AGG | + | chr4.4:18008907-18008926 | None:intergenic | 40.0% |
| !! | GCAGTTCATTCTGAAGTTCA+GGG | - | chr4.4:18008655-18008674 | MS.gene34976:intron | 40.0% |
| !! | GTGATATTCTGGGATTGTAG+AGG | + | chr4.4:18008910-18008929 | None:intergenic | 40.0% |
| !! | TATTCTGGGATTGTAGAGGA+GGG | + | chr4.4:18008906-18008925 | None:intergenic | 40.0% |
| !! | TGCAGTTCATTCTGAAGTTC+AGG | - | chr4.4:18008654-18008673 | MS.gene34976:intron | 40.0% |
| ACCACCATCATCATCATCAG+AGG | - | chr4.4:18007895-18007914 | MS.gene34976:CDS | 45.0% | |
| ACTAGGCTAAGGGACCAATT+TGG | - | chr4.4:18008210-18008229 | MS.gene34976:intron | 45.0% | |
| ATGGCAAAGCATGCTTTGCA+TGG | - | chr4.4:18008064-18008083 | MS.gene34976:CDS | 45.0% | |
| CCACCATCATCATCATCAGA+GGG | - | chr4.4:18007896-18007915 | MS.gene34976:CDS | 45.0% | |
| GCTAGAAAGAGAAGCTGCAA+AGG | + | chr4.4:18008420-18008439 | None:intergenic | 45.0% | |
| GCTCCATAGGATGTTCAACT+GGG | + | chr4.4:18007944-18007963 | None:intergenic | 45.0% | |
| TCCTGATGATCCATGAGTGA+GGG | + | chr4.4:18008845-18008864 | None:intergenic | 45.0% | |
| TTTCCAAGTGCTTCAGCAGT+TGG | - | chr4.4:18008933-18008952 | MS.gene34976:CDS | 45.0% | |
| ! | CAACCCTCTGATGATGATGA+TGG | + | chr4.4:18007902-18007921 | None:intergenic | 45.0% |
| !! | TCAGGCATTGGACATTTCAC+CGG | + | chr4.4:18007984-18008003 | None:intergenic | 45.0% |
| !! | TCTGATGATGATGATGGTGG+TGG | + | chr4.4:18007896-18007915 | None:intergenic | 45.0% |
| ACCCTCACTCATGGATCATC+AGG | - | chr4.4:18008841-18008860 | MS.gene34976:CDS | 50.0% | |
| AGGAAGAGAGGTGAGATGTC+TGG | - | chr4.4:18008751-18008770 | MS.gene34976:intron | 50.0% | |
| CTCCTGATGATCCATGAGTG+AGG | + | chr4.4:18008846-18008865 | None:intergenic | 50.0% | |
| GATTGTAGAGGAGGGAGATG+TGG | + | chr4.4:18008898-18008917 | None:intergenic | 50.0% | |
| GGAAGAGAGGTGAGATGTCT+GGG | - | chr4.4:18008752-18008771 | MS.gene34976:intron | 50.0% | |
| GGCTCCATAGGATGTTCAAC+TGG | + | chr4.4:18007945-18007964 | None:intergenic | 50.0% | |
| TGAACATCCTATGGAGCCAC+CGG | - | chr4.4:18007947-18007966 | MS.gene34976:CDS | 50.0% | |
| TGGACAAAGAACAGCTGGCA+TGG | - | chr4.4:18008777-18008796 | MS.gene34976:intron | 50.0% | |
| TTCACCGGACGATCTTCATC+CGG | + | chr4.4:18007969-18007988 | None:intergenic | 50.0% | |
| ! | TGTCCAACTGCTGAAGCACT+TGG | + | chr4.4:18008939-18008958 | None:intergenic | 50.0% |
| !! | CCCTCTGATGATGATGATGG+TGG | + | chr4.4:18007899-18007918 | None:intergenic | 50.0% |
| AGAGGTGAGATGTCTGGGTC+TGG | - | chr4.4:18008757-18008776 | MS.gene34976:intron | 55.0% | |
| GGGTCTGGACAAAGAACAGC+TGG | - | chr4.4:18008772-18008791 | MS.gene34976:intron | 55.0% | |
| TCTTCATCCGGTGGCTCCAT+AGG | + | chr4.4:18007957-18007976 | None:intergenic | 55.0% | |
| ACCGGACGATCTTCATCCGG+TGG | + | chr4.4:18007966-18007985 | None:intergenic | 60.0% | |
| CGGCATCACACCCTCACTCA+TGG | - | chr4.4:18008832-18008851 | MS.gene34976:CDS | 60.0% | |
| CTTTCGCACTCCTCGAGCTG+GGG | + | chr4.4:18008812-18008831 | None:intergenic | 60.0% | |
| GCCACCGGATGAAGATCGTC+CGG | - | chr4.4:18007962-18007981 | MS.gene34976:CDS | 60.0% | |
| GCTTTCGCACTCCTCGAGCT+GGG | + | chr4.4:18008813-18008832 | None:intergenic | 60.0% | |
| ! | CAGCTCGAGGAGTGCGAAAG+CGG | - | chr4.4:18008812-18008831 | MS.gene34976:intron | 60.0% |
| CGCTTTCGCACTCCTCGAGC+TGG | + | chr4.4:18008814-18008833 | None:intergenic | 65.0% | |
| GACACAGATGCCCCAGCTCG+AGG | - | chr4.4:18008799-18008818 | MS.gene34976:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 18007876 | 18008972 | 18007876 | ID=MS.gene34976 |
| chr4.4 | mRNA | 18007876 | 18008972 | 18007876 | ID=MS.gene34976.t1;Parent=MS.gene34976 |
| chr4.4 | exon | 18008829 | 18008972 | 18008829 | ID=MS.gene34976.t1.exon1;Parent=MS.gene34976.t1 |
| chr4.4 | CDS | 18008829 | 18008972 | 18008829 | ID=cds.MS.gene34976.t1;Parent=MS.gene34976.t1 |
| chr4.4 | exon | 18007876 | 18008136 | 18007876 | ID=MS.gene34976.t1.exon2;Parent=MS.gene34976.t1 |
| chr4.4 | CDS | 18007876 | 18008136 | 18007876 | ID=cds.MS.gene34976.t1;Parent=MS.gene34976.t1 |
| Gene Sequence |
| Protein sequence |
