AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35473


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35473.t1 MTR_3g075030 92.797 236 10 2 1 229 1 236 2.27e-160 443
MS.gene35473.t1 MTR_4g103840 46.718 259 90 11 1 224 17 262 6.22e-55 177
MS.gene35473.t1 MTR_6g023190 32.463 268 126 9 7 224 28 290 4.49e-25 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35473.t1 AT3G48510 41.696 283 97 11 1 224 21 294 1.69e-44 150
MS.gene35473.t1 AT5G50360 38.989 277 108 12 1 224 21 289 1.30e-43 148
MS.gene35473.t1 AT5G63350 37.500 288 110 10 1 224 21 302 5.79e-39 137
MS.gene35473.t1 AT5G40800 35.156 256 127 10 2 224 21 270 1.59e-30 114
MS.gene35473.t1 AT3G27250 34.470 264 125 8 4 224 22 280 1.48e-28 109
MS.gene35473.t1 AT3G27250 34.470 264 125 8 4 224 22 280 1.48e-28 109
MS.gene35473.t1 AT5G40790 33.571 280 125 10 4 224 24 301 3.13e-25 100

Find 73 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATTTATATCGGACGGTTA+TGG 0.272493 3.4:+73620461 MS.gene35473:CDS
AGTAGAGGATCTGGAGTTTC+GGG 0.311049 3.4:-73620206 None:intergenic
GATTTATATCGGACGGTTAT+GGG 0.323289 3.4:+73620462 MS.gene35473:CDS
AAGGACAAGTTTGGTTGGTT+AGG 0.328445 3.4:+73620528 MS.gene35473:CDS
GAGGAAGGAAGTAGAGGATC+TGG 0.372917 3.4:-73620215 None:intergenic
AAGTAGAGGATCTGGAGTTT+CGG 0.378062 3.4:-73620207 None:intergenic
CGAGGAGAGGAAAGTGAAAC+TGG 0.397568 3.4:+73620421 MS.gene35473:CDS
GTGGAGGAATAGTGCATATA+GGG 0.406861 3.4:+73620493 MS.gene35473:CDS
GATCTTACAGCGGTAGGGTT+AGG 0.420934 3.4:+73620311 MS.gene35473:CDS
TCGGAGGCGGTGCATGGCTT+AGG 0.448865 3.4:-73620068 None:intergenic
AGGACAAGTTTGGTTGGTTA+GGG 0.450227 3.4:+73620529 MS.gene35473:CDS
GAATAGTGCATATAGGGAAA+TGG 0.450785 3.4:+73620499 MS.gene35473:CDS
GGCAGCATGGAGATGAAGGC+TGG 0.454057 3.4:+73620372 MS.gene35473:CDS
AAGTGGACAGAATCATGTTA+AGG 0.454487 3.4:+73620030 MS.gene35473:CDS
CGTGGAGGAATAGTGCATAT+AGG 0.458439 3.4:+73620492 MS.gene35473:CDS
TAACATGATTCTGTCCACTT+TGG 0.465109 3.4:-73620027 None:intergenic
AACTGTCCCTCCACCGGCGG+TGG 0.469426 3.4:+73620166 MS.gene35473:CDS
ACTGGAGAAGGACACGTGTC+CGG 0.470490 3.4:+73620439 MS.gene35473:CDS
CTGGAGAAGGACACGTGTCC+GGG 0.485377 3.4:+73620440 MS.gene35473:CDS
GTGAGAGTACAGTACGCGTA+TGG 0.487916 3.4:+73620584 MS.gene35473:CDS
GCCGTGCGATGCGTGGAGAT+TGG 0.491883 3.4:+73620622 MS.gene35473:CDS
TGGTGGGTGAAGGACAAGTT+TGG 0.508774 3.4:+73620519 MS.gene35473:CDS
GTGAAAGAGGATTCACGTGC+AGG 0.513519 3.4:+73620561 MS.gene35473:CDS
CGTGAATCCTCTTTCACTGT+AGG 0.519583 3.4:-73620555 None:intergenic
TCGACGTCGGAGGCGGTGCA+TGG 0.520028 3.4:-73620074 None:intergenic
AGAGTCACCACCGCCGGTGG+AGG 0.532600 3.4:-73620173 None:intergenic
CACGTGTCCGGGATTTATAT+CGG 0.538547 3.4:+73620451 MS.gene35473:CDS
GGGTGAAGGACAAGTTTGGT+TGG 0.538666 3.4:+73620523 MS.gene35473:CDS
CTGGAGTTTCGGGAAGCAGC+GGG 0.540339 3.4:-73620196 None:intergenic
CTTACAGCGGTAGGGTTAGG+AGG 0.546084 3.4:+73620314 MS.gene35473:CDS
AAGGTGGATCTTACAGCGGT+AGG 0.548232 3.4:+73620305 MS.gene35473:CDS
CCTTCTCCGATGTGGTAGCA+TGG 0.556821 3.4:-73620286 None:intergenic
AAAAGCGTCGACGTCGGAGG+CGG 0.558175 3.4:-73620081 None:intergenic
ACGTGGCAGCATGGAGATGA+AGG 0.562247 3.4:+73620368 MS.gene35473:CDS
AGGGTACCCTACAGTGAAAG+AGG 0.563763 3.4:+73620548 MS.gene35473:CDS
TAACCGTCCGATATAAATCC+CGG 0.564401 3.4:-73620458 None:intergenic
TAGTGCATATAGGGAAATGG+TGG 0.564825 3.4:+73620502 MS.gene35473:CDS
GAAGAGTGCCGTGCGATGCG+TGG 0.568763 3.4:+73620615 MS.gene35473:CDS
AGGTGGATCTTACAGCGGTA+GGG 0.572112 3.4:+73620306 MS.gene35473:CDS
TGCTACCACATCGGAGAAGG+TGG 0.572613 3.4:+73620289 MS.gene35473:CDS
AGCGGGAGAGTCACCACCGC+CGG 0.578527 3.4:-73620179 None:intergenic
TATAGGGAAATGGTGGGTGA+AGG 0.578922 3.4:+73620509 MS.gene35473:CDS
GAGTCACCACCGCCGGTGGA+GGG 0.581071 3.4:-73620172 None:intergenic
GTGCATGGCTTAGGAGCGAT+AGG 0.582617 3.4:-73620059 None:intergenic
GTGAATCCTCTTTCACTGTA+GGG 0.584545 3.4:-73620554 None:intergenic
AGAGTAGAGACGACGAGGAG+AGG 0.588357 3.4:+73620408 MS.gene35473:CDS
CCATGCTACCACATCGGAGA+AGG 0.589985 3.4:+73620286 MS.gene35473:CDS
TGAGAGTACAGTACGCGTAT+GGG 0.590021 3.4:+73620585 MS.gene35473:CDS
TTGGCTGAGGAAGGAAGTAG+AGG 0.590328 3.4:-73620221 None:intergenic
AGCTTACGAAATGTCCAAAG+TGG 0.592157 3.4:+73620013 None:intergenic
TCCAATCTCCACGCATCGCA+CGG 0.601495 3.4:-73620623 None:intergenic
TGAAAGAGGATTCACGTGCA+GGG 0.607088 3.4:+73620562 MS.gene35473:CDS
GCAGCATGGAGATGAAGGCT+GGG 0.611472 3.4:+73620373 MS.gene35473:CDS
TGTCTGAGCTTCGAGAATCA+CGG 0.611686 3.4:+73620263 MS.gene35473:CDS
TCTGGAGTTTCGGGAAGCAG+CGG 0.617319 3.4:-73620197 None:intergenic
TTCTCGAAGCTCAGACACAC+CGG 0.622372 3.4:-73620257 None:intergenic
GAGGAAAGTGAAACTGGAGA+AGG 0.622774 3.4:+73620427 MS.gene35473:CDS
AGTGCATATAGGGAAATGGT+GGG 0.624526 3.4:+73620503 MS.gene35473:CDS
ATGGAGATGAAGGCTGGGAG+TGG 0.626519 3.4:+73620378 MS.gene35473:CDS
CTAGAAAGGGAAACAACTCA+AGG 0.628678 3.4:-73620690 None:intergenic
AAGATCCACCTTCTCCGATG+TGG 0.639110 3.4:-73620294 None:intergenic
GCGTGGAGATTGGACTGTGG+TGG 0.643557 3.4:+73620632 MS.gene35473:CDS
GGGAGAGTCACCACCGCCGG+TGG 0.643642 3.4:-73620176 None:intergenic
TGTCCGGGATTTATATCGGA+CGG 0.666853 3.4:+73620455 MS.gene35473:CDS
ACGGTTATGGGAGAGTGACG+TGG 0.666874 3.4:+73620474 MS.gene35473:CDS
AAAACAAACGGTGTAACCGT+CGG 0.690022 3.4:+73620138 MS.gene35473:CDS
TCACGGCCATGCTACCACAT+CGG 0.701017 3.4:+73620280 MS.gene35473:CDS
GATGAAGAGTAGAGACGACG+AGG 0.704591 3.4:+73620403 MS.gene35473:CDS
GTAACTGACACGTGGCAGCA+TGG 0.705335 3.4:+73620359 MS.gene35473:CDS
GTTATGGGAGAGTGACGTGG+AGG 0.729117 3.4:+73620477 MS.gene35473:CDS
TTGAATGCGTAACTGACACG+TGG 0.739002 3.4:+73620351 MS.gene35473:CDS
GGAGAAGGTGGATCTTACAG+CGG 0.748589 3.4:+73620301 MS.gene35473:CDS
GATGCGTGGAGATTGGACTG+TGG 0.766424 3.4:+73620629 MS.gene35473:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATCACTCGAAAAACAAA+CGG + chr3.4:73620126-73620145 MS.gene35473:CDS 25.0%
AAGTGGACAGAATCATGTTA+AGG + chr3.4:73620030-73620049 MS.gene35473:CDS 35.0%
AGCCAAAAACACAAACAAAC+CGG + chr3.4:73620238-73620257 MS.gene35473:CDS 35.0%
GAATAGTGCATATAGGGAAA+TGG + chr3.4:73620499-73620518 MS.gene35473:CDS 35.0%
GATTTATATCGGACGGTTAT+GGG + chr3.4:73620462-73620481 MS.gene35473:CDS 35.0%
TAACATGATTCTGTCCACTT+TGG - chr3.4:73620030-73620049 None:intergenic 35.0%
!!! GTGATTTTTTCTAGATCCGT+CGG - chr3.4:73620114-73620133 None:intergenic 35.0%
!!! TGATTTTTTCTAGATCCGTC+GGG - chr3.4:73620113-73620132 None:intergenic 35.0%
!!! TTTGTTTGTGTTTTTGGCTG+AGG - chr3.4:73620237-73620256 None:intergenic 35.0%
AAAACAAACGGTGTAACCGT+CGG + chr3.4:73620138-73620157 MS.gene35473:CDS 40.0%
AGTGCATATAGGGAAATGGT+GGG + chr3.4:73620503-73620522 MS.gene35473:CDS 40.0%
CTAGAAAGGGAAACAACTCA+AGG - chr3.4:73620693-73620712 None:intergenic 40.0%
GGATTTATATCGGACGGTTA+TGG + chr3.4:73620461-73620480 MS.gene35473:CDS 40.0%
GTGAATCCTCTTTCACTGTA+GGG - chr3.4:73620557-73620576 None:intergenic 40.0%
GTGGAGGAATAGTGCATATA+GGG + chr3.4:73620493-73620512 MS.gene35473:CDS 40.0%
TAACCGTCCGATATAAATCC+CGG - chr3.4:73620461-73620480 None:intergenic 40.0%
TAGTGCATATAGGGAAATGG+TGG + chr3.4:73620502-73620521 MS.gene35473:CDS 40.0%
! AAGGACAAGTTTGGTTGGTT+AGG + chr3.4:73620528-73620547 MS.gene35473:CDS 40.0%
! AAGTAGAGGATCTGGAGTTT+CGG - chr3.4:73620210-73620229 None:intergenic 40.0%
! AGGACAAGTTTGGTTGGTTA+GGG + chr3.4:73620529-73620548 MS.gene35473:CDS 40.0%
!!! CACCGGTTTGTTTGTGTTTT+TGG - chr3.4:73620243-73620262 None:intergenic 40.0%
!!! TTTGTGTTTTTGGCTGAGGA+AGG - chr3.4:73620233-73620252 None:intergenic 40.0%
!!! TTTTGAAAAAGCGTCGACGT+CGG - chr3.4:73620090-73620109 None:intergenic 40.0%
CACGTGTCCGGGATTTATAT+CGG + chr3.4:73620451-73620470 MS.gene35473:CDS 45.0%
CGTGAATCCTCTTTCACTGT+AGG - chr3.4:73620558-73620577 None:intergenic 45.0%
CGTGGAGGAATAGTGCATAT+AGG + chr3.4:73620492-73620511 MS.gene35473:CDS 45.0%
GAGGAAAGTGAAACTGGAGA+AGG + chr3.4:73620427-73620446 MS.gene35473:CDS 45.0%
TGAAAGAGGATTCACGTGCA+GGG + chr3.4:73620562-73620581 MS.gene35473:CDS 45.0%
TGTCCGGGATTTATATCGGA+CGG + chr3.4:73620455-73620474 MS.gene35473:CDS 45.0%
TGTCTGAGCTTCGAGAATCA+CGG + chr3.4:73620263-73620282 MS.gene35473:CDS 45.0%
TTGAATGCGTAACTGACACG+TGG + chr3.4:73620351-73620370 MS.gene35473:CDS 45.0%
! AGTAGAGGATCTGGAGTTTC+GGG - chr3.4:73620209-73620228 None:intergenic 45.0%
! TGAGAGTACAGTACGCGTAT+GGG + chr3.4:73620585-73620604 MS.gene35473:CDS 45.0%
!! TATAGGGAAATGGTGGGTGA+AGG + chr3.4:73620509-73620528 MS.gene35473:CDS 45.0%
!!! GACGCTTTTTCAAAACCCGA+CGG + chr3.4:73620095-73620114 MS.gene35473:CDS 45.0%
AAGATCCACCTTCTCCGATG+TGG - chr3.4:73620297-73620316 None:intergenic 50.0%
AAGGTGGATCTTACAGCGGT+AGG + chr3.4:73620305-73620324 MS.gene35473:CDS 50.0%
AGGTGGATCTTACAGCGGTA+GGG + chr3.4:73620306-73620325 MS.gene35473:CDS 50.0%
CGAGGAGAGGAAAGTGAAAC+TGG + chr3.4:73620421-73620440 MS.gene35473:CDS 50.0%
GAGAAAAACTGTCCCTCCAC+CGG + chr3.4:73620160-73620179 MS.gene35473:CDS 50.0%
GAGGAAGGAAGTAGAGGATC+TGG - chr3.4:73620218-73620237 None:intergenic 50.0%
GATCTTACAGCGGTAGGGTT+AGG + chr3.4:73620311-73620330 MS.gene35473:CDS 50.0%
GATGAAGAGTAGAGACGACG+AGG + chr3.4:73620403-73620422 MS.gene35473:CDS 50.0%
GGAGAAGGTGGATCTTACAG+CGG + chr3.4:73620301-73620320 MS.gene35473:CDS 50.0%
GGGTGAAGGACAAGTTTGGT+TGG + chr3.4:73620523-73620542 MS.gene35473:CDS 50.0%
GTGAAAGAGGATTCACGTGC+AGG + chr3.4:73620561-73620580 MS.gene35473:CDS 50.0%
TTCTCGAAGCTCAGACACAC+CGG - chr3.4:73620260-73620279 None:intergenic 50.0%
TTGGCTGAGGAAGGAAGTAG+AGG - chr3.4:73620224-73620243 None:intergenic 50.0%
! AGGGTACCCTACAGTGAAAG+AGG + chr3.4:73620548-73620567 MS.gene35473:CDS 50.0%
! GAGGGACAGTTTTTCTCCGA+CGG - chr3.4:73620157-73620176 None:intergenic 50.0%
! GTGAGAGTACAGTACGCGTA+TGG + chr3.4:73620584-73620603 MS.gene35473:CDS 50.0%
! TGGTGGGTGAAGGACAAGTT+TGG + chr3.4:73620519-73620538 MS.gene35473:CDS 50.0%
!! TGGACTGTGGTGGTTTTGCA+TGG + chr3.4:73620642-73620661 MS.gene35473:CDS 50.0%
AAAAACTGTCCCTCCACCGG+CGG + chr3.4:73620163-73620182 MS.gene35473:CDS 55.0%
ACGGTTATGGGAGAGTGACG+TGG + chr3.4:73620474-73620493 MS.gene35473:CDS 55.0%
ACGTGGCAGCATGGAGATGA+AGG + chr3.4:73620368-73620387 MS.gene35473:CDS 55.0%
ACTGGAGAAGGACACGTGTC+CGG + chr3.4:73620439-73620458 MS.gene35473:CDS 55.0%
AGAGTAGAGACGACGAGGAG+AGG + chr3.4:73620408-73620427 MS.gene35473:CDS 55.0%
ATGGAGATGAAGGCTGGGAG+TGG + chr3.4:73620378-73620397 MS.gene35473:CDS 55.0%
CCATGCTACCACATCGGAGA+AGG + chr3.4:73620286-73620305 MS.gene35473:CDS 55.0%
CCTTCTCCGATGTGGTAGCA+TGG - chr3.4:73620289-73620308 None:intergenic 55.0%
CTTACAGCGGTAGGGTTAGG+AGG + chr3.4:73620314-73620333 MS.gene35473:CDS 55.0%
GATGCGTGGAGATTGGACTG+TGG + chr3.4:73620629-73620648 MS.gene35473:CDS 55.0%
GCAGCATGGAGATGAAGGCT+GGG + chr3.4:73620373-73620392 MS.gene35473:CDS 55.0%
GTAACTGACACGTGGCAGCA+TGG + chr3.4:73620359-73620378 MS.gene35473:CDS 55.0%
GTGCATGGCTTAGGAGCGAT+AGG - chr3.4:73620062-73620081 None:intergenic 55.0%
GTTATGGGAGAGTGACGTGG+AGG + chr3.4:73620477-73620496 MS.gene35473:CDS 55.0%
TCACGGCCATGCTACCACAT+CGG + chr3.4:73620280-73620299 MS.gene35473:CDS 55.0%
TCCAATCTCCACGCATCGCA+CGG - chr3.4:73620626-73620645 None:intergenic 55.0%
TGCTACCACATCGGAGAAGG+TGG + chr3.4:73620289-73620308 MS.gene35473:CDS 55.0%
! TCTGGAGTTTCGGGAAGCAG+CGG - chr3.4:73620200-73620219 None:intergenic 55.0%
!! TGAAAAAGCGTCGACGTCGG+AGG - chr3.4:73620087-73620106 None:intergenic 55.0%
CTGGAGAAGGACACGTGTCC+GGG + chr3.4:73620440-73620459 MS.gene35473:CDS 60.0%
CTGGAGTTTCGGGAAGCAGC+GGG - chr3.4:73620199-73620218 None:intergenic 60.0%
GCGTGGAGATTGGACTGTGG+TGG + chr3.4:73620632-73620651 MS.gene35473:CDS 60.0%
GGCAGCATGGAGATGAAGGC+TGG + chr3.4:73620372-73620391 MS.gene35473:CDS 60.0%
!! AAAAGCGTCGACGTCGGAGG+CGG - chr3.4:73620084-73620103 None:intergenic 60.0%
GCCGTGCGATGCGTGGAGAT+TGG + chr3.4:73620622-73620641 MS.gene35473:CDS 65.0%
TCGGAGGCGGTGCATGGCTT+AGG - chr3.4:73620071-73620090 None:intergenic 65.0%
!! GAAGAGTGCCGTGCGATGCG+TGG + chr3.4:73620615-73620634 MS.gene35473:CDS 65.0%
AACTGTCCCTCCACCGGCGG+TGG + chr3.4:73620166-73620185 MS.gene35473:CDS 70.0%
AGAGTCACCACCGCCGGTGG+AGG - chr3.4:73620176-73620195 None:intergenic 70.0%
AGCGGGAGAGTCACCACCGC+CGG - chr3.4:73620182-73620201 None:intergenic 70.0%
GAGTCACCACCGCCGGTGGA+GGG - chr3.4:73620175-73620194 None:intergenic 70.0%
TCGACGTCGGAGGCGGTGCA+TGG - chr3.4:73620077-73620096 None:intergenic 70.0%
GGGAGAGTCACCACCGCCGG+TGG - chr3.4:73620179-73620198 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 73620023 73620712 73620023 ID=MS.gene35473
chr3.4 mRNA 73620023 73620712 73620023 ID=MS.gene35473.t1;Parent=MS.gene35473
chr3.4 exon 73620023 73620712 73620023 ID=MS.gene35473.t1.exon1;Parent=MS.gene35473.t1
chr3.4 CDS 73620023 73620712 73620023 ID=cds.MS.gene35473.t1;Parent=MS.gene35473.t1
Gene Sequence

>MS.gene35473

ATGTCCAAAGTGGACAGAATCATGTTAAGGTTTCGTCCTATCGCTCCTAAGCCATGCACCGCCTCCGACGTCGACGCTTTTTCAAAACCCGACGGATCTAGAAAAAATCACTCGAAAAACAAACGGTGTAACCGTCGGAGAAAAACTGTCCCTCCACCGGCGGTGGTGACTCTCCCGCTGCTTCCCGAAACTCCAGATCCTCTACTTCCTTCCTCAGCCAAAAACACAAACAAACCGGTGTGTCTGAGCTTCGAGAATCACGGCCATGCTACCACATCGGAGAAGGTGGATCTTACAGCGGTAGGGTTAGGAGGATCAGTCGTAACAGTTGAATGCGTAACTGACACGTGGCAGCATGGAGATGAAGGCTGGGAGTGGTTGATGAAGAGTAGAGACGACGAGGAGAGGAAAGTGAAACTGGAGAAGGACACGTGTCCGGGATTTATATCGGACGGTTATGGGAGAGTGACGTGGAGGAATAGTGCATATAGGGAAATGGTGGGTGAAGGACAAGTTTGGTTGGTTAGGGTACCCTACAGTGAAAGAGGATTCACGTGCAGGGTGAGAGTACAGTACGCGTATGGGAAAGAGAGAAGAGTGCCGTGCGATGCGTGGAGATTGGACTGTGGTGGTTTTGCATGGAGATTAGATGTTACTGCTGCTCTTGCCTTGAGTTGTTTCCCTTTCTAG

Protein sequence

>MS.gene35473.t1

MSKVDRIMLRFRPIAPKPCTASDVDAFSKPDGSRKNHSKNKRCNRRRKTVPPPAVVTLPLLPETPDPLLPSSAKNTNKPVCLSFENHGHATTSEKVDLTAVGLGGSVVTVECVTDTWQHGDEGWEWLMKSRDDEERKVKLEKDTCPGFISDGYGRVTWRNSAYREMVGEGQVWLVRVPYSERGFTCRVRVQYAYGKERRVPCDAWRLDCGGFAWRLDVTAALALSCFPF