AlfalfaGEDB | MS.gene055912

M. sativa cultivar XinJiangDaYe / MS.gene055912

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055912.t1	XP_003590484.1	99	102	1	0	1	102	118	219	5.50E-51	209.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055912.t1	Q8RUN2	88.9	90	10	0	1	90	113	202	7.5e-41	167.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055912.t1	G7IBJ6	99.0	102	1	0	1	102	118	219	4.0e-51	209.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene055912	0.821993	3.00E-53	-1.69E-46
MS.gene049340	MS.gene055912	0.807446	5.03E-50	-1.69E-46
MS.gene049341	MS.gene055912	0.812437	4.24E-51	-1.69E-46
MS.gene050020	MS.gene055912	0.813614	2.34E-51	-1.69E-46
MS.gene051328	MS.gene055912	0.816309	5.91E-52	-1.69E-46
MS.gene052133	MS.gene055912	0.808723	2.69E-50	-1.69E-46
MS.gene055162	MS.gene055912	0.803854	2.86E-49	-1.69E-46
MS.gene055912	MS.gene057705	0.802765	4.80E-49	-1.69E-46
MS.gene055912	MS.gene061176	0.821341	4.25E-53	-1.69E-46
MS.gene055912	MS.gene061177	0.819917	9.03E-53	-1.69E-46
MS.gene055912	MS.gene061178	0.82164	3.63E-53	-1.69E-46
MS.gene055912	MS.gene061947	0.844683	6.49E-59	-1.69E-46
MS.gene055912	MS.gene06371	0.805047	1.61E-49	-1.69E-46
MS.gene055912	MS.gene066105	0.812876	3.40E-51	-1.69E-46
MS.gene055912	MS.gene06645	0.802605	5.18E-49	-1.69E-46
MS.gene055912	MS.gene067521	0.811053	8.48E-51	-1.69E-46
MS.gene055912	MS.gene07100	0.808468	3.05E-50	-1.69E-46
MS.gene055912	MS.gene073785	0.808792	2.60E-50	-1.69E-46
MS.gene055912	MS.gene08428	0.817327	3.49E-52	-1.69E-46
MS.gene055912	MS.gene09873	0.821351	4.23E-53	-1.69E-46
MS.gene055912	MS.gene21614	0.802652	5.07E-49	-1.69E-46
MS.gene055912	MS.gene23058	0.819476	1.14E-52	-1.69E-46
MS.gene055912	MS.gene24610	0.947936	2.54E-106	-1.69E-46
MS.gene055912	MS.gene25435	0.804416	2.18E-49	-1.69E-46
MS.gene055912	MS.gene27668	0.810348	1.21E-50	-1.69E-46
MS.gene055912	MS.gene29880	0.815278	1.00E-51	-1.69E-46
MS.gene055912	MS.gene34369	0.801589	8.38E-49	-1.69E-46
MS.gene055912	MS.gene35048	0.814137	1.80E-51	-1.69E-46
MS.gene055912	MS.gene35214	0.808773	2.63E-50	-1.69E-46
MS.gene055912	MS.gene35473	0.801223	9.96E-49	-1.69E-46
MS.gene055912	MS.gene40595	0.827624	1.41E-54	-1.69E-46
MS.gene055912	MS.gene40919	0.810359	1.20E-50	-1.69E-46
MS.gene055912	MS.gene44141	0.8167	4.83E-52	-1.69E-46
MS.gene055912	MS.gene45479	0.822015	2.97E-53	-1.69E-46
MS.gene055912	MS.gene54919	0.813453	2.54E-51	-1.69E-46
MS.gene055912	MS.gene55887	0.802171	6.37E-49	-1.69E-46
MS.gene055912	MS.gene61727	0.80327	3.78E-49	-1.69E-46
MS.gene055912	MS.gene61729	0.80864	2.80E-50	-1.69E-46
MS.gene055912	MS.gene63032	0.818287	2.12E-52	-1.69E-46
MS.gene055912	MS.gene64101	0.815054	1.12E-51	-1.69E-46
MS.gene055912	MS.gene64650	0.802842	4.63E-49	-1.69E-46
MS.gene055912	MS.gene68136	0.814013	1.91E-51	-1.69E-46
MS.gene055912	MS.gene68735	0.8266	2.48E-54	-1.69E-46
MS.gene055912	MS.gene69717	0.800258	1.57E-48	-1.69E-46
MS.gene055912	MS.gene70398	0.807853	4.13E-50	-1.69E-46
MS.gene055912	MS.gene71215	0.837996	3.74E-57	-1.69E-46
MS.gene055912	MS.gene72904	0.80436	2.24E-49	-1.69E-46
MS.gene055912	MS.gene75604	0.822106	2.83E-53	-1.69E-46
MS.gene055912	MS.gene88680	0.807206	5.66E-50	-1.69E-46
MS.gene055912	MS.gene89562	0.806492	8.01E-50	-1.69E-46
MS.gene055912	MS.gene90981	0.828752	7.55E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 26 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTACATGCAGGTGGTTA+TGG	0.290968	1.1:+48651368	None:intergenic
ACAATTATGTGGCGCGCATT+TGG	0.351381	1.1:-48651635	None:intergenic
CTTGAGATGTCATACTCTTC+AGG	0.376180	1.1:-48651861	None:intergenic
AAAAGAGTTAGTGAAGAAAC+TGG	0.405307	1.1:+48651673	MS.gene055912:CDS
AATTACATGCAGGTGGTTAT+GGG	0.415602	1.1:+48651369	None:intergenic
GTCATAACCTGGGCTCAACT+TGG	0.447889	1.1:+48651413	MS.gene055912:CDS
GTGGGACTAGTAAATGTTGA+TGG	0.489170	1.1:+48651554	MS.gene055912:CDS
TGACTTCAAGAAGCTCCTCT+AGG	0.523303	1.1:-48651394	None:intergenic
ACTTGGGATTCATGACAAAC+CGG	0.526362	1.1:+48651430	MS.gene055912:CDS
CATCGACAAAGCTGTGGAAG+AGG	0.532010	1.1:+48651601	MS.gene055912:CDS
AGCTTCTTGAAGTCATAACC+TGG	0.532925	1.1:+48651402	MS.gene055912:CDS
GCGTTGCTTCGAAGTTGAGC+TGG	0.538596	1.1:+48651814	MS.gene055912:CDS
GAAGCAACGCCTTCGTGACA+AGG	0.544836	1.1:-48651801	None:intergenic
GACTTCAAGAAGCTCCTCTA+GGG	0.553887	1.1:-48651393	None:intergenic
TCATAACCTGGGCTCAACTT+GGG	0.556522	1.1:+48651414	MS.gene055912:CDS
CTGAAGAGTATGACATCTCA+AGG	0.559781	1.1:+48651862	MS.gene055912:CDS
TTCGAAGTTGAGCTGGCAGA+TGG	0.564883	1.1:+48651821	MS.gene055912:CDS
TTCATTCATCGACAAAGCTG+TGG	0.574795	1.1:+48651595	MS.gene055912:CDS
ATGAATCCCAAGTTGAGCCC+AGG	0.585691	1.1:-48651420	None:intergenic
ATCGACAAAGCTGTGGAAGA+GGG	0.594245	1.1:+48651602	MS.gene055912:CDS
GATGTCATACTCTTCAGGAT+AGG	0.595686	1.1:-48651856	None:intergenic
GCTTCTTGAAGTCATAACCT+GGG	0.658696	1.1:+48651403	MS.gene055912:CDS
GATTATGTTCCTTGTCACGA+AGG	0.661791	1.1:+48651792	MS.gene055912:CDS
TAGGTGCTGATACAATTATG+TGG	0.681813	1.1:-48651646	None:intergenic
AGAGTTAGTGAAGAAACTGG+AGG	0.710150	1.1:+48651676	MS.gene055912:CDS
AGGTGGTTATGGGACCCTAG+AGG	0.728214	1.1:+48651379	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TGAATTAGTTAATAATATTT+AGG	-	chr1.1:48651485-48651504	None:intergenic	10.0%
!	AATGTCAAATCTAGTTAAAC+TGG	-	chr1.1:48651521-48651540	None:intergenic	25.0%
!	TATTATGTTTGATTCATCAC+AGG	+	chr1.1:48651770-48651789	MS.gene055912:intron	25.0%
!!	ATTTAGGTTCCTTAACAAAA+TGG	-	chr1.1:48651469-48651488	None:intergenic	25.0%
	AAAAGAGTTAGTGAAGAAAC+TGG	+	chr1.1:48651673-48651692	MS.gene055912:CDS	30.0%
	AAATGGTACAAGTCAATTAC+CGG	-	chr1.1:48651452-48651471	None:intergenic	30.0%
!	TTCACTAACTCTTTTGATGT+AGG	-	chr1.1:48651668-48651687	None:intergenic	30.0%
!!	TGACTTGTACCATTTTGTTA+AGG	+	chr1.1:48651457-48651476	MS.gene055912:intron	30.0%
!!	TAGGTGCTGATACAATTATG+TGG	-	chr1.1:48651649-48651668	None:intergenic	35.0%
	ACTTGGGATTCATGACAAAC+CGG	+	chr1.1:48651430-48651449	MS.gene055912:CDS	40.0%
	AGAGTATGACATCTCAAGGT+AGG	+	chr1.1:48651866-48651885	MS.gene055912:CDS	40.0%
	AGAGTTAGTGAAGAAACTGG+AGG	+	chr1.1:48651676-48651695	MS.gene055912:CDS	40.0%
	AGCTTCTTGAAGTCATAACC+TGG	+	chr1.1:48651402-48651421	MS.gene055912:CDS	40.0%
	CTTGAGATGTCATACTCTTC+AGG	-	chr1.1:48651864-48651883	None:intergenic	40.0%
	GATGTCATACTCTTCAGGAT+AGG	-	chr1.1:48651859-48651878	None:intergenic	40.0%
	GATTATGTTCCTTGTCACGA+AGG	+	chr1.1:48651792-48651811	MS.gene055912:CDS	40.0%
	GCTTCTTGAAGTCATAACCT+GGG	+	chr1.1:48651403-48651422	MS.gene055912:CDS	40.0%
	TTCATTCATCGACAAAGCTG+TGG	+	chr1.1:48651595-48651614	MS.gene055912:CDS	40.0%
!	CTGAAGAGTATGACATCTCA+AGG	+	chr1.1:48651862-48651881	MS.gene055912:CDS	40.0%
!	GTGGGACTAGTAAATGTTGA+TGG	+	chr1.1:48651554-48651573	MS.gene055912:CDS	40.0%
	ACAATTATGTGGCGCGCATT+TGG	-	chr1.1:48651638-48651657	None:intergenic	45.0%
	ATCGACAAAGCTGTGGAAGA+GGG	+	chr1.1:48651602-48651621	MS.gene055912:CDS	45.0%
	GACTTCAAGAAGCTCCTCTA+GGG	-	chr1.1:48651396-48651415	None:intergenic	45.0%
	TCATAACCTGGGCTCAACTT+GGG	+	chr1.1:48651414-48651433	MS.gene055912:CDS	45.0%
	TGACTTCAAGAAGCTCCTCT+AGG	-	chr1.1:48651397-48651416	None:intergenic	45.0%
	TTTGACATTCCACAACTCGC+AGG	+	chr1.1:48651532-48651551	MS.gene055912:intron	45.0%
	ACATTCCACAACTCGCAGGT+GGG	+	chr1.1:48651536-48651555	MS.gene055912:intron	50.0%
	CATCGACAAAGCTGTGGAAG+AGG	+	chr1.1:48651601-48651620	MS.gene055912:CDS	50.0%
	GTCATAACCTGGGCTCAACT+TGG	+	chr1.1:48651413-48651432	MS.gene055912:CDS	50.0%
	TTCGAAGTTGAGCTGGCAGA+TGG	+	chr1.1:48651821-48651840	MS.gene055912:CDS	50.0%
!	ATGAATCCCAAGTTGAGCCC+AGG	-	chr1.1:48651423-48651442	None:intergenic	50.0%
	GAAGCAACGCCTTCGTGACA+AGG	-	chr1.1:48651804-48651823	None:intergenic	55.0%
	GACATTCCACAACTCGCAGG+TGG	+	chr1.1:48651535-48651554	MS.gene055912:intron	55.0%
	GCGTTGCTTCGAAGTTGAGC+TGG	+	chr1.1:48651814-48651833	MS.gene055912:CDS	55.0%
	CTAGTCCCACCTGCGAGTTG+TGG	-	chr1.1:48651544-48651563	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	48651381	48651887	48651381	ID=MS.gene055912
chr1.1	mRNA	48651381	48651887	48651381	ID=MS.gene055912.t1;Parent=MS.gene055912
chr1.1	exon	48651381	48651451	48651381	ID=MS.gene055912.t1.exon1;Parent=MS.gene055912.t1
chr1.1	CDS	48651381	48651451	48651381	ID=cds.MS.gene055912.t1;Parent=MS.gene055912.t1
chr1.1	exon	48651554	48651697	48651554	ID=MS.gene055912.t1.exon2;Parent=MS.gene055912.t1
chr1.1	CDS	48651554	48651697	48651554	ID=cds.MS.gene055912.t1;Parent=MS.gene055912.t1
chr1.1	exon	48651792	48651887	48651792	ID=MS.gene055912.t1.exon3;Parent=MS.gene055912.t1
chr1.1	CDS	48651792	48651887	48651792	ID=cds.MS.gene055912.t1;Parent=MS.gene055912.t1

Gene Sequence

>MS.gene055912

GTGGTTATGGGACCCTAGAGGAGCTTCTTGAAGTCATAACCTGGGCTCAACTTGGGATTCATGACAAACCGGTAATTGACTTGTACCATTTTGTTAAGGAACCTAAATATTATTAACTAATTCACATTGTGCTATTACCAGTTTAACTAGATTTGACATTCCACAACTCGCAGGTGGGACTAGTAAATGTTGATGGATACTTTAATTCATTACTTTCATTCATCGACAAAGCTGTGGAAGAGGGATTTATCAGTCCAAATGCGCGCCACATAATTGTATCAGCACCTACATCAAAAGAGTTAGTGAAGAAACTGGAGGTAAAATCAAAGTGTTTGTATATGCAATCACATTCTTTTCTTCAGCAGAATTAAGCAAAAATGATATATATTTATTATGTTTGATTCATCACAGGATTATGTTCCTTGTCACGAAGGCGTTGCTTCGAAGTTGAGCTGGCAGATGGAACAAGAGCTAGCCTATCCTGAAGAGTATGACATCTCAAGGTAG

Protein sequence

>MS.gene055912.t1

VVMGPURSFLKSUPGLNLGFMTNRWDUUMLMDTLIHYFHSSTKLWKRDLSVQMRATULYQHLHQKSUURNWRIMFLVTKALLRSUAGRWNKSUPILKSMTSQG