Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35777.t1 | XP_003597047.1 | 98.7 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 2.10E-81 | 311.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35777.t1 | G7IKM8 | 98.7 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 1.5e-81 | 311.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049239 | MS.gene35777 | 0.811081 | 8.37E-51 | -1.69E-46 |
| MS.gene049298 | MS.gene35777 | 0.843471 | 1.37E-58 | -1.69E-46 |
| MS.gene050710 | MS.gene35777 | 0.804356 | 2.25E-49 | -1.69E-46 |
| MS.gene051539 | MS.gene35777 | 0.810007 | 1.43E-50 | -1.69E-46 |
| MS.gene051838 | MS.gene35777 | -0.816512 | 5.32E-52 | -1.69E-46 |
| MS.gene053108 | MS.gene35777 | 0.829788 | 4.23E-55 | -1.69E-46 |
| MS.gene054941 | MS.gene35777 | 0.824607 | 7.36E-54 | -1.69E-46 |
| MS.gene056387 | MS.gene35777 | -0.811718 | 6.08E-51 | -1.69E-46 |
| MS.gene05652 | MS.gene35777 | 0.805311 | 1.42E-49 | -1.69E-46 |
| MS.gene056618 | MS.gene35777 | 0.832915 | 7.20E-56 | -1.69E-46 |
| MS.gene057669 | MS.gene35777 | 0.819009 | 1.45E-52 | -1.69E-46 |
| MS.gene057717 | MS.gene35777 | 0.801198 | 1.01E-48 | -1.69E-46 |
| MS.gene058128 | MS.gene35777 | 0.815361 | 9.61E-52 | -1.69E-46 |
| MS.gene058853 | MS.gene35777 | 0.814118 | 1.81E-51 | -1.69E-46 |
| MS.gene05926 | MS.gene35777 | 0.809636 | 1.72E-50 | -1.69E-46 |
| MS.gene05960 | MS.gene35777 | 0.800819 | 1.21E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35777.t1 | MTR_2g089050 | 98.734 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 7.73e-113 | 317 |
| MS.gene35777.t1 | MTR_4g045673 | 70.253 | 158 | 47 | 0 | 1 | 158 | 1 | 158 | 6.25e-80 | 234 |
| MS.gene35777.t1 | MTR_4g092130 | 60.127 | 158 | 63 | 0 | 1 | 158 | 1 | 158 | 5.69e-71 | 211 |
| MS.gene35777.t1 | MTR_2g010840 | 60.127 | 158 | 63 | 0 | 1 | 158 | 1 | 158 | 1.25e-61 | 188 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene35777.t1 | AT4G21310 | 68.354 | 158 | 50 | 0 | 1 | 158 | 1 | 158 | 2.21e-78 | 230 |
| MS.gene35777.t1 | AT2G32280 | 62.025 | 158 | 60 | 0 | 1 | 158 | 1 | 158 | 1.37e-71 | 213 |
| MS.gene35777.t1 | AT2G32280 | 62.025 | 158 | 60 | 0 | 1 | 158 | 1 | 158 | 1.37e-71 | 213 |
| MS.gene35777.t1 | AT4G21310 | 65.000 | 120 | 42 | 0 | 39 | 158 | 1 | 120 | 2.04e-54 | 168 |
| MS.gene35777.t1 | AT1G11500 | 41.212 | 165 | 90 | 1 | 1 | 158 | 1 | 165 | 4.92e-36 | 123 |
| MS.gene35777.t1 | AT1G11500 | 41.212 | 165 | 90 | 1 | 1 | 158 | 6 | 170 | 6.57e-36 | 123 |
| MS.gene35777.t1 | AT1G11500 | 43.860 | 114 | 64 | 0 | 45 | 158 | 24 | 137 | 1.90e-27 | 100 |
| MS.gene35777.t1 | AT1G05291 | 38.776 | 147 | 83 | 3 | 12 | 155 | 23 | 165 | 1.44e-23 | 92.0 |
Find 37 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTCATAAGGCCTTCATGTT+TGG | 0.331948 | 2.1:+12993124 | MS.gene35777:CDS |
| GATGTCACAGCTGGGATTCT+TGG | 0.346927 | 2.1:+12992754 | MS.gene35777:CDS |
| ACCGCAGCCACCTAGCAAAT+TGG | 0.354925 | 2.1:-12993188 | None:intergenic |
| GCCATTGAGAGTTGCCTATT+GGG | 0.372087 | 2.1:-12993247 | None:intergenic |
| GGCCATTGAGAGTTGCCTAT+TGG | 0.393490 | 2.1:-12993248 | None:intergenic |
| ATGTCTCTTGATTGTTATCA+TGG | 0.396765 | 2.1:+12992732 | MS.gene35777:CDS |
| GGATTGAGCATGTTGGTGAT+AGG | 0.405472 | 2.1:+12993616 | MS.gene35777:CDS |
| TTCCTTAGTCACTAATTAAG+AGG | 0.431827 | 2.1:-12993764 | None:intergenic |
| GTTGTTGATCAGGATTGTTT+TGG | 0.434468 | 2.1:+12993588 | MS.gene35777:intron |
| GACCATCACTTTCTATCCAT+TGG | 0.452040 | 2.1:+12993682 | MS.gene35777:CDS |
| GCCAATTTGCTAGGTGGCTG+CGG | 0.471857 | 2.1:+12993187 | MS.gene35777:CDS |
| ACCCAATAGGCAACTCTCAA+TGG | 0.473987 | 2.1:+12993246 | MS.gene35777:CDS |
| TTCAAAAGTCTTCACCCAAT+AGG | 0.481487 | 2.1:+12993233 | MS.gene35777:CDS |
| GTTATCATGGATGTCACAGC+TGG | 0.486047 | 2.1:+12992745 | MS.gene35777:CDS |
| TCAGAAATGGCTAGAGAAGT+TGG | 0.488463 | 2.1:+12992700 | None:intergenic |
| AGGTTAAACACTTGAGATTG+TGG | 0.506672 | 2.1:+12993080 | MS.gene35777:intron |
| ATCACTTTCTATCCATTGGT+GGG | 0.512449 | 2.1:+12993686 | MS.gene35777:CDS |
| AACATGAAGGCCTTATGACT+TGG | 0.519268 | 2.1:-12993121 | None:intergenic |
| CATGTTATAGCCAATTTGCT+AGG | 0.526723 | 2.1:+12993178 | MS.gene35777:CDS |
| GTGTAGAGATCCAAGTCATA+AGG | 0.530738 | 2.1:+12993111 | MS.gene35777:CDS |
| GATTGAGCATGTTGGTGATA+GGG | 0.548783 | 2.1:+12993617 | MS.gene35777:CDS |
| CATCACTTTCTATCCATTGG+TGG | 0.556658 | 2.1:+12993685 | MS.gene35777:CDS |
| GGCTGTTGGATTGAGCATGT+TGG | 0.561637 | 2.1:+12993609 | MS.gene35777:CDS |
| AAAACAAACAACAGATCAAG+TGG | 0.563378 | 2.1:+12993643 | MS.gene35777:CDS |
| CTAGCAAATTGGCTATAACA+TGG | 0.567612 | 2.1:-12993177 | None:intergenic |
| AGTCACTAATTAAGAGGCAG+TGG | 0.571955 | 2.1:-12993758 | None:intergenic |
| TATATTACCATGTTAAAATG+AGG | 0.574693 | 2.1:-12993273 | None:intergenic |
| AAAACACAAAATCCCACCAA+TGG | 0.578066 | 2.1:-12993698 | None:intergenic |
| TAGCAAATTGGCTATAACAT+GGG | 0.592154 | 2.1:-12993176 | None:intergenic |
| AGCTGAAATTGCTCAAAACA+AGG | 0.596775 | 2.1:+12992783 | MS.gene35777:CDS |
| TTACCATGTTAAAATGAGGA+TGG | 0.599472 | 2.1:-12993269 | None:intergenic |
| TGCAGCTAATCCAAACATGA+AGG | 0.600785 | 2.1:-12993134 | None:intergenic |
| AACAGATCAAGTGGTACTTG+TGG | 0.611029 | 2.1:+12993652 | MS.gene35777:CDS |
| GTTATAGCCAATTTGCTAGG+TGG | 0.649884 | 2.1:+12993181 | MS.gene35777:CDS |
| TTATCATGGATGTCACAGCT+GGG | 0.651085 | 2.1:+12992746 | MS.gene35777:CDS |
| AAGAAACACAGAATAGACCA+TGG | 0.662268 | 2.1:-12993723 | None:intergenic |
| CACCAATGGATAGAAAGTGA+TGG | 0.663881 | 2.1:-12993684 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTTATTTTACAAAATC+AGG | + | chr2.1:12993060-12993079 | MS.gene35777:intron | 10.0% |
| !! | AAAATTAATGTACATTATCA+TGG | - | chr2.1:12992984-12993003 | None:intergenic | 15.0% |
| !! | TATATTACCATGTTAAAATG+AGG | - | chr2.1:12993276-12993295 | None:intergenic | 20.0% |
| !!! | AAATTCAGTGTTTTAAAAAC+CGG | - | chr2.1:12993492-12993511 | None:intergenic | 20.0% |
| !!! | GTTTTATAGAATTAGAAACA+TGG | - | chr2.1:12992828-12992847 | None:intergenic | 20.0% |
| ! | CAACAACGTAATTAAAACAA+TGG | - | chr2.1:12993575-12993594 | None:intergenic | 25.0% |
| ! | TCAATCACAGTACAATTATT+AGG | - | chr2.1:12992942-12992961 | None:intergenic | 25.0% |
| !!! | TTTTAATTACGTTGTTGATC+AGG | + | chr2.1:12993578-12993597 | MS.gene35777:intron | 25.0% |
| AAAACAAACAACAGATCAAG+TGG | + | chr2.1:12993643-12993662 | MS.gene35777:CDS | 30.0% | |
| ACCATGATAAAAAGTTCCAT+TGG | + | chr2.1:12993453-12993472 | MS.gene35777:intron | 30.0% | |
| ATGTCTCTTGATTGTTATCA+TGG | + | chr2.1:12992732-12992751 | MS.gene35777:CDS | 30.0% | |
| CATTTATTAGTGAACTCTCA+TGG | - | chr2.1:12993539-12993558 | None:intergenic | 30.0% | |
| GAAAACAAGTAGAGGAATTA+GGG | - | chr2.1:12993300-12993319 | None:intergenic | 30.0% | |
| TAGCAAATTGGCTATAACAT+GGG | - | chr2.1:12993179-12993198 | None:intergenic | 30.0% | |
| TTACCATGTTAAAATGAGGA+TGG | - | chr2.1:12993272-12993291 | None:intergenic | 30.0% | |
| TTCAACTGCTTACAAAACTA+TGG | - | chr2.1:12993416-12993435 | None:intergenic | 30.0% | |
| ! | ACCAATGGAACTTTTTATCA+TGG | - | chr2.1:12993457-12993476 | None:intergenic | 30.0% |
| !!! | TAGTTTTGTAAGCAGTTGAA+CGG | + | chr2.1:12993416-12993435 | MS.gene35777:intron | 30.0% |
| AAAACACAAAATCCCACCAA+TGG | - | chr2.1:12993701-12993720 | None:intergenic | 35.0% | |
| AAGAAACACAGAATAGACCA+TGG | - | chr2.1:12993726-12993745 | None:intergenic | 35.0% | |
| ACATTGTCGAAAACAAGTAG+AGG | - | chr2.1:12993308-12993327 | None:intergenic | 35.0% | |
| AGCTGAAATTGCTCAAAACA+AGG | + | chr2.1:12992783-12992802 | MS.gene35777:CDS | 35.0% | |
| AGGTTAAACACTTGAGATTG+TGG | + | chr2.1:12993080-12993099 | MS.gene35777:intron | 35.0% | |
| ATCACTTTCTATCCATTGGT+GGG | + | chr2.1:12993686-12993705 | MS.gene35777:CDS | 35.0% | |
| CGAAAACAAGTAGAGGAATT+AGG | - | chr2.1:12993301-12993320 | None:intergenic | 35.0% | |
| CTAGCAAATTGGCTATAACA+TGG | - | chr2.1:12993180-12993199 | None:intergenic | 35.0% | |
| TTCAAAAGTCTTCACCCAAT+AGG | + | chr2.1:12993233-12993252 | MS.gene35777:CDS | 35.0% | |
| ! | CATGTTATAGCCAATTTGCT+AGG | + | chr2.1:12993178-12993197 | MS.gene35777:CDS | 35.0% |
| ! | GTTGTTGATCAGGATTGTTT+TGG | + | chr2.1:12993588-12993607 | MS.gene35777:intron | 35.0% |
| ! | TGTCTAGGGTTTGGTTATTT+AGG | + | chr2.1:12993382-12993401 | MS.gene35777:intron | 35.0% |
| !! | CATTGGTTCAATATTCAGTC+CGG | + | chr2.1:12993470-12993489 | MS.gene35777:intron | 35.0% |
| AACAGATCAAGTGGTACTTG+TGG | + | chr2.1:12993652-12993671 | MS.gene35777:CDS | 40.0% | |
| AGCAGTTGAACGGTTTAAAG+AGG | + | chr2.1:12993426-12993445 | MS.gene35777:intron | 40.0% | |
| AGTCATAAGGCCTTCATGTT+TGG | + | chr2.1:12993124-12993143 | MS.gene35777:CDS | 40.0% | |
| CACCAATGGATAGAAAGTGA+TGG | - | chr2.1:12993687-12993706 | None:intergenic | 40.0% | |
| CATCACTTTCTATCCATTGG+TGG | + | chr2.1:12993685-12993704 | MS.gene35777:CDS | 40.0% | |
| CGGACTGAATATTGAACCAA+TGG | - | chr2.1:12993472-12993491 | None:intergenic | 40.0% | |
| GACCATCACTTTCTATCCAT+TGG | + | chr2.1:12993682-12993701 | MS.gene35777:CDS | 40.0% | |
| GTGTAGAGATCCAAGTCATA+AGG | + | chr2.1:12993111-12993130 | MS.gene35777:CDS | 40.0% | |
| TGCAGCTAATCCAAACATGA+AGG | - | chr2.1:12993137-12993156 | None:intergenic | 40.0% | |
| TTATCATGGATGTCACAGCT+GGG | + | chr2.1:12992746-12992765 | MS.gene35777:CDS | 40.0% | |
| ! | AACATGAAGGCCTTATGACT+TGG | - | chr2.1:12993124-12993143 | None:intergenic | 40.0% |
| ! | GGGATTTTGTGTTTTGTCCA+TGG | + | chr2.1:12993706-12993725 | MS.gene35777:CDS | 40.0% |
| !! | GATTGAGCATGTTGGTGATA+GGG | + | chr2.1:12993617-12993636 | MS.gene35777:CDS | 40.0% |
| !! | GTTATAGCCAATTTGCTAGG+TGG | + | chr2.1:12993181-12993200 | MS.gene35777:CDS | 40.0% |
| !! | TGGCCATCCTCATTTTAACA+TGG | + | chr2.1:12993266-12993285 | MS.gene35777:CDS | 40.0% |
| !!! | ATCAGGATTGTTTTGGCTGT+TGG | + | chr2.1:12993595-12993614 | MS.gene35777:intron | 40.0% |
| ACCCAATAGGCAACTCTCAA+TGG | + | chr2.1:12993246-12993265 | MS.gene35777:CDS | 45.0% | |
| ACTATGAAACCGCACTGTCT+AGG | + | chr2.1:12993367-12993386 | MS.gene35777:intron | 45.0% | |
| CTATGAAACCGCACTGTCTA+GGG | + | chr2.1:12993368-12993387 | MS.gene35777:intron | 45.0% | |
| GCCATTGAGAGTTGCCTATT+GGG | - | chr2.1:12993250-12993269 | None:intergenic | 45.0% | |
| GTTATCATGGATGTCACAGC+TGG | + | chr2.1:12992745-12992764 | MS.gene35777:CDS | 45.0% | |
| TAACCAAACCCTAGACAGTG+CGG | - | chr2.1:12993379-12993398 | None:intergenic | 45.0% | |
| !! | GGATTGAGCATGTTGGTGAT+AGG | + | chr2.1:12993616-12993635 | MS.gene35777:CDS | 45.0% |
| GATGTCACAGCTGGGATTCT+TGG | + | chr2.1:12992754-12992773 | MS.gene35777:CDS | 50.0% | |
| GGCCATTGAGAGTTGCCTAT+TGG | - | chr2.1:12993251-12993270 | None:intergenic | 50.0% | |
| GGCTGTTGGATTGAGCATGT+TGG | + | chr2.1:12993609-12993628 | MS.gene35777:CDS | 50.0% | |
| ! | AAACCGCACTGTCTAGGGTT+TGG | + | chr2.1:12993373-12993392 | MS.gene35777:intron | 50.0% |
| ACCGCAGCCACCTAGCAAAT+TGG | - | chr2.1:12993191-12993210 | None:intergenic | 55.0% | |
| !! | GCCAATTTGCTAGGTGGCTG+CGG | + | chr2.1:12993187-12993206 | MS.gene35777:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 12992706 | 12993771 | 12992706 | ID=MS.gene35777 |
| chr2.1 | mRNA | 12992706 | 12993771 | 12992706 | ID=MS.gene35777.t1;Parent=MS.gene35777 |
| chr2.1 | exon | 12992706 | 12992804 | 12992706 | ID=MS.gene35777.t1.exon1;Parent=MS.gene35777.t1 |
| chr2.1 | CDS | 12992706 | 12992804 | 12992706 | ID=cds.MS.gene35777.t1;Parent=MS.gene35777.t1 |
| chr2.1 | exon | 12993082 | 12993287 | 12993082 | ID=MS.gene35777.t1.exon2;Parent=MS.gene35777.t1 |
| chr2.1 | CDS | 12993082 | 12993287 | 12993082 | ID=cds.MS.gene35777.t1;Parent=MS.gene35777.t1 |
| chr2.1 | exon | 12993600 | 12993771 | 12993600 | ID=MS.gene35777.t1.exon3;Parent=MS.gene35777.t1 |
| chr2.1 | CDS | 12993600 | 12993771 | 12993600 | ID=cds.MS.gene35777.t1;Parent=MS.gene35777.t1 |
| Gene Sequence |
| Protein sequence |
