AlfalfaGEDB | MS.gene35821

M. sativa cultivar XinJiangDaYe / MS.gene35821

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35821.t1	XP_003596984.2	95.1	123	5	1	1	122	1	123	3.40E-47	197.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35821.t1	G7ITZ1	95.1	123	5	1	1	122	1	123	2.4e-47	197.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051914	MS.gene35821	0.818427	1.97E-52	-1.69E-46
MS.gene054018	MS.gene35821	0.989493	2.34E-178	-1.69E-46
MS.gene054633	MS.gene35821	0.802631	5.12E-49	-1.69E-46
MS.gene054662	MS.gene35821	0.863409	2.54E-64	-1.69E-46
MS.gene05640	MS.gene35821	0.803287	3.75E-49	-1.69E-46
MS.gene057367	MS.gene35821	0.813857	2.07E-51	-1.69E-46
MS.gene060291	MS.gene35821	0.804391	2.21E-49	-1.69E-46
MS.gene06093	MS.gene35821	0.807713	4.42E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35821.t1	MTR_2g088320	96.748	123	3	1	1	122	1	123	8.38e-82	235

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene35821.t1	AT5G54970	44.706	85	44	2	13	94	15	99	2.74e-13	62.0

Find 50 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGGTGGTGGTGGTGAGGC+TGG	0.218611	2.1:+13334520	None:intergenic
CGGAGGCGAGCTAGGGTTCC+TGG	0.289851	2.1:+13334610	None:intergenic
ACCATCGGCGGCTGCGGCGG+CGG	0.308022	2.1:+13334480	None:intergenic
GATCTTGGTCTTGAATCATT+TGG	0.367487	2.1:-13334343	MS.gene35821:CDS
GGTTTCCCTTCCTTCTTCTC+CGG	0.381260	2.1:-13334460	MS.gene35821:CDS
GCTGCTGACTCAGCAGATCT+TGG	0.404060	2.1:-13334358	MS.gene35821:CDS
AGGTCTTGTTCTCTGCTTCA+TGG	0.408985	2.1:+13334666	None:intergenic
TGGAAGTGGTTTGGTAAAGT+AGG	0.419142	2.1:+13334646	None:intergenic
GGTGGTGGTGGTGAGGCTGG+TGG	0.422166	2.1:+13334523	None:intergenic
TGATCGGAGGGAGGTGGTGG+TGG	0.422451	2.1:+13334511	None:intergenic
GTTTCCCTTCCTTCTTCTCC+GGG	0.422834	2.1:-13334459	MS.gene35821:CDS
TTGAGTCACGGAGGCGAGCT+AGG	0.435623	2.1:+13334602	None:intergenic
AGGGAAACCATCGGCGGCTG+CGG	0.436360	2.1:+13334474	None:intergenic
GCGGCTGCGGCGGCGGTTTG+AGG	0.436624	2.1:+13334487	None:intergenic
GGAGTTGAGACGGTGAGATC+TGG	0.440317	2.1:+13334570	None:intergenic
TCCTTCTTCTCCGGGATCTA+CGG	0.444986	2.1:-13334451	MS.gene35821:CDS
GAGCTAGGGTTCCTGGTTTG+AGG	0.446447	2.1:+13334617	None:intergenic
GGCTGATCGGAGGGAGGTGG+TGG	0.453951	2.1:+13334508	None:intergenic
ACCGTAGATCCCGGAGAAGA+AGG	0.460453	2.1:+13334450	None:intergenic
ACCGCCGCCGCAGCCGCCGA+TGG	0.468049	2.1:-13334481	MS.gene35821:CDS
TTCCTGGTTTGAGGAATTTG+TGG	0.469740	2.1:+13334626	None:intergenic
AGGAATTTGTGGAAGTGGTT+TGG	0.473781	2.1:+13334637	None:intergenic
GTTTGAGGAATTTGTGGAAG+TGG	0.474784	2.1:+13334632	None:intergenic
TCTGCTGAGTCAGCAGCAGC+AGG	0.484937	2.1:+13334364	None:intergenic
CTTGGTCTTGAATCATTTGG+TGG	0.505567	2.1:-13334340	MS.gene35821:CDS
GAAACCATCGGCGGCTGCGG+CGG	0.505671	2.1:+13334477	None:intergenic
TCAGCAGCAGCAGGACTACC+AGG	0.510522	2.1:+13334373	None:intergenic
TGAGGCTGATCGGAGGGAGG+TGG	0.511381	2.1:+13334505	None:intergenic
GTGGTGGTGAGGCTGGTGGA+CGG	0.514928	2.1:+13334527	None:intergenic
GGAGGGAGGTGGTGGTGGTG+AGG	0.526828	2.1:+13334516	None:intergenic
GCGACGAGTTTCTTCCGTTG+TGG	0.540155	2.1:+13334418	None:intergenic
GCGGTTTGAGGCTGATCGGA+GGG	0.544351	2.1:+13334499	None:intergenic
TGAGTCACGGAGGCGAGCTA+GGG	0.544597	2.1:+13334603	None:intergenic
TAGATCCCGGAGAAGAAGGA+AGG	0.559260	2.1:+13334454	None:intergenic
GGCGGCGGTTTGAGGCTGAT+CGG	0.567721	2.1:+13334495	None:intergenic
GGCGGTTTGAGGCTGATCGG+AGG	0.568671	2.1:+13334498	None:intergenic
TGGCGGTTATCTTTGAGTCA+CGG	0.583959	2.1:+13334590	None:intergenic
TCTGAGAGAGGGAGTTGAGA+CGG	0.588321	2.1:+13334560	None:intergenic
ACATCGAGGACCGTAGATCC+CGG	0.588493	2.1:+13334441	None:intergenic
GTTTGAGGCTGATCGGAGGG+AGG	0.605220	2.1:+13334502	None:intergenic
TTCCACAAATTCCTCAAACC+AGG	0.613859	2.1:-13334628	MS.gene35821:CDS
GAAGAAGGAAGGGAAACCAT+CGG	0.615545	2.1:+13334465	None:intergenic
TTCTTCCGTTGTGGACATCG+AGG	0.627074	2.1:+13334427	None:intergenic
AGATCCCGGAGAAGAAGGAA+GGG	0.635957	2.1:+13334455	None:intergenic
GTAGATTGAGATCTGAGAGA+GGG	0.642846	2.1:+13334549	None:intergenic
GGTAGATTGAGATCTGAGAG+AGG	0.644225	2.1:+13334548	None:intergenic
GTTGAGACGGTGAGATCTGG+CGG	0.649621	2.1:+13334573	None:intergenic
ACGGTCCTCGATGTCCACAA+CGG	0.679494	2.1:-13334432	MS.gene35821:CDS
CGGTTATCTTTGAGTCACGG+AGG	0.737996	2.1:+13334593	None:intergenic
GAAGGAAGGGAAACCATCGG+CGG	0.745154	2.1:+13334468	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGTCAGTTTTCTTTGTTCC+TGG	-	chr2.1:13334591-13334610	MS.gene35821:CDS	35.0%
!!	GATCTTGGTCTTGAATCATT+TGG	-	chr2.1:13334639-13334658	MS.gene35821:CDS	35.0%
	AGGAATTTGTGGAAGTGGTT+TGG	+	chr2.1:13334348-13334367	None:intergenic	40.0%
	GTAGATTGAGATCTGAGAGA+GGG	+	chr2.1:13334436-13334455	None:intergenic	40.0%
	GTTTGAGGAATTTGTGGAAG+TGG	+	chr2.1:13334353-13334372	None:intergenic	40.0%
	TTCCACAAATTCCTCAAACC+AGG	-	chr2.1:13334354-13334373	MS.gene35821:CDS	40.0%
!	TTCCTGGTTTGAGGAATTTG+TGG	+	chr2.1:13334359-13334378	None:intergenic	40.0%
!!	CTTGGTCTTGAATCATTTGG+TGG	-	chr2.1:13334642-13334661	MS.gene35821:CDS	40.0%
!!	TGGAAGTGGTTTGGTAAAGT+AGG	+	chr2.1:13334339-13334358	None:intergenic	40.0%
	GAAGAAGGAAGGGAAACCAT+CGG	+	chr2.1:13334520-13334539	None:intergenic	45.0%
	GGTAGATTGAGATCTGAGAG+AGG	+	chr2.1:13334437-13334456	None:intergenic	45.0%
	TGGCGGTTATCTTTGAGTCA+CGG	+	chr2.1:13334395-13334414	None:intergenic	45.0%
	AGATCCCGGAGAAGAAGGAA+GGG	+	chr2.1:13334530-13334549	None:intergenic	50.0%
	CGGTTATCTTTGAGTCACGG+AGG	+	chr2.1:13334392-13334411	None:intergenic	50.0%
	GGTTTCCCTTCCTTCTTCTC+CGG	-	chr2.1:13334522-13334541	MS.gene35821:CDS	50.0%
	GTTTCCCTTCCTTCTTCTCC+GGG	-	chr2.1:13334523-13334542	MS.gene35821:CDS	50.0%
	TAGATCCCGGAGAAGAAGGA+AGG	+	chr2.1:13334531-13334550	None:intergenic	50.0%
	TCCTTCTTCTCCGGGATCTA+CGG	-	chr2.1:13334531-13334550	MS.gene35821:CDS	50.0%
	TCTGAGAGAGGGAGTTGAGA+CGG	+	chr2.1:13334425-13334444	None:intergenic	50.0%
	TTCTTCCGTTGTGGACATCG+AGG	+	chr2.1:13334558-13334577	None:intergenic	50.0%
	ACATCGAGGACCGTAGATCC+CGG	+	chr2.1:13334544-13334563	None:intergenic	55.0%
	ACCGTAGATCCCGGAGAAGA+AGG	+	chr2.1:13334535-13334554	None:intergenic	55.0%
	ACGGTCCTCGATGTCCACAA+CGG	-	chr2.1:13334550-13334569	MS.gene35821:CDS	55.0%
	GAAGGAAGGGAAACCATCGG+CGG	+	chr2.1:13334517-13334536	None:intergenic	55.0%
	GCGACGAGTTTCTTCCGTTG+TGG	+	chr2.1:13334567-13334586	None:intergenic	55.0%
	GGAGTTGAGACGGTGAGATC+TGG	+	chr2.1:13334415-13334434	None:intergenic	55.0%
	GTTGAGACGGTGAGATCTGG+CGG	+	chr2.1:13334412-13334431	None:intergenic	55.0%
!	GAGCTAGGGTTCCTGGTTTG+AGG	+	chr2.1:13334368-13334387	None:intergenic	55.0%
!!	GCTGCTGACTCAGCAGATCT+TGG	-	chr2.1:13334624-13334643	MS.gene35821:CDS	55.0%
	TCAGCAGCAGCAGGACTACC+AGG	+	chr2.1:13334612-13334631	None:intergenic	60.0%
	TCTGCTGAGTCAGCAGCAGC+AGG	+	chr2.1:13334621-13334640	None:intergenic	60.0%
	TGAGTCACGGAGGCGAGCTA+GGG	+	chr2.1:13334382-13334401	None:intergenic	60.0%
	TTGAGTCACGGAGGCGAGCT+AGG	+	chr2.1:13334383-13334402	None:intergenic	60.0%
!	GCGGTTTGAGGCTGATCGGA+GGG	+	chr2.1:13334486-13334505	None:intergenic	60.0%
!	GTTTGAGGCTGATCGGAGGG+AGG	+	chr2.1:13334483-13334502	None:intergenic	60.0%
	AGGGAAACCATCGGCGGCTG+CGG	+	chr2.1:13334511-13334530	None:intergenic	65.0%
	TGAGGCTGATCGGAGGGAGG+TGG	+	chr2.1:13334480-13334499	None:intergenic	65.0%
!	GGCGGCGGTTTGAGGCTGAT+CGG	+	chr2.1:13334490-13334509	None:intergenic	65.0%
!	GGCGGTTTGAGGCTGATCGG+AGG	+	chr2.1:13334487-13334506	None:intergenic	65.0%
!	GTGGTGGTGAGGCTGGTGGA+CGG	+	chr2.1:13334458-13334477	None:intergenic	65.0%
!	TGATCGGAGGGAGGTGGTGG+TGG	+	chr2.1:13334474-13334493	None:intergenic	65.0%
	CGGAGGCGAGCTAGGGTTCC+TGG	+	chr2.1:13334375-13334394	None:intergenic	70.0%
	GAAACCATCGGCGGCTGCGG+CGG	+	chr2.1:13334508-13334527	None:intergenic	70.0%
	GGCTGATCGGAGGGAGGTGG+TGG	+	chr2.1:13334477-13334496	None:intergenic	70.0%
!!	GGAGGGAGGTGGTGGTGGTG+AGG	+	chr2.1:13334469-13334488	None:intergenic	70.0%
!!	GGAGGTGGTGGTGGTGAGGC+TGG	+	chr2.1:13334465-13334484	None:intergenic	70.0%
!!	GGTGGTGGTGGTGAGGCTGG+TGG	+	chr2.1:13334462-13334481	None:intergenic	70.0%
!	ACCATCGGCGGCTGCGGCGG+CGG	+	chr2.1:13334505-13334524	None:intergenic	80.0%
!	GCGGCTGCGGCGGCGGTTTG+AGG	+	chr2.1:13334498-13334517	None:intergenic	80.0%
!	ACCGCCGCCGCAGCCGCCGA+TGG	-	chr2.1:13334501-13334520	MS.gene35821:CDS	85.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	13334318	13334686	13334318	ID=MS.gene35821
chr2.1	mRNA	13334318	13334686	13334318	ID=MS.gene35821.t1;Parent=MS.gene35821
chr2.1	exon	13334318	13334686	13334318	ID=MS.gene35821.t1.exon1;Parent=MS.gene35821.t1
chr2.1	CDS	13334318	13334686	13334318	ID=cds.MS.gene35821.t1;Parent=MS.gene35821.t1

Gene Sequence	>MS.gene35821 ATGAAGCAGAGAACAAGACCTACTTTACCAAACCACTTCCACAAATTCCTCAAACCAGGAACCCTAGCTCGCCTCCGTGACTCAAAGATAACCGCCAGATCTCACCGTCTCAACTCCCTCTCTCAGATCTCAATCTACCGTCCACCAGCCTCACCACCACCACCTCCCTCCGATCAGCCTCAAACCGCCGCCGCAGCCGCCGATGGTTTCCCTTCCTTCTTCTCCGGGATCTACGGTCCTCGATGTCCACAACGGAAGAAACTCGTCGCTGCAAAGTCAGTTTTCTTTGTTCCTGGTAGTCCTGCTGCTGCTGACTCAGCAGATCTTGGTCTTGAATCATTTGGTGGCGATATTATCGCTGCTAATTGA
Protein sequence	>MS.gene35821.t1 MKQRTRPTLPNHFHKFLKPGTLARLRDSKITARSHRLNSLSQISIYRPPASPPPPPSDQPQTAAAAADGFPSFFSGIYGPRCPQRKKLVAAKSVFFVPGSPAAADSADLGLESFGGDIIAAN