AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35962

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35962.t1 MTR_8g055840 99.344 610 4 0 1 610 1 610 0.0 1270
MS.gene35962.t1 MTR_5g040360 84.590 610 93 1 1 610 1 609 0.0 1108
MS.gene35962.t1 MTR_3g040370 71.757 609 166 4 2 609 3 606 0.0 946
MS.gene35962.t1 MTR_4g098890 68.874 604 174 5 2 597 3 600 0.0 912
MS.gene35962.t1 MTR_8g100075 54.160 613 251 6 17 608 25 628 0.0 712
MS.gene35962.t1 MTR_7g076170 55.263 608 245 6 17 601 23 626 0.0 711
MS.gene35962.t1 MTR_0019s0120 54.803 635 247 9 3 609 13 635 0.0 708
MS.gene35962.t1 MTR_1g101370 57.383 596 240 8 18 601 25 618 0.0 708
MS.gene35962.t1 MTR_8g432680 52.883 607 262 6 17 601 27 631 0.0 680
MS.gene35962.t1 MTR_7g006060 52.964 523 234 4 84 594 43 565 0.0 585
MS.gene35962.t1 MTR_0019s0120 52.809 534 212 9 3 508 13 534 0.0 562
MS.gene35962.t1 MTR_8g071280 45.660 530 278 7 84 607 70 595 2.50e-166 488
MS.gene35962.t1 MTR_8g432620 44.821 531 265 8 82 600 86 600 5.63e-164 481
MS.gene35962.t1 MTR_7g076150 45.018 542 270 8 71 600 62 587 2.19e-163 479
MS.gene35962.t1 MTR_5g026930 42.833 600 313 10 9 600 13 590 4.46e-163 479
MS.gene35962.t1 MTR_5g080720 43.178 535 279 11 75 597 116 637 8.46e-154 457
MS.gene35962.t1 MTR_1g442840 41.096 584 302 12 20 575 18 587 3.63e-150 447
MS.gene35962.t1 MTR_1g075900 42.755 559 286 12 73 610 79 624 9.48e-148 441
MS.gene35962.t1 MTR_3g068990 44.465 533 263 12 82 598 151 666 1.30e-147 442
MS.gene35962.t1 MTR_1g069785 42.910 536 273 10 87 602 65 587 1.75e-147 439
MS.gene35962.t1 MTR_1g442840 45.607 478 234 9 111 575 10 474 1.28e-146 434
MS.gene35962.t1 MTR_1g029730 44.234 529 271 12 84 599 141 658 4.11e-145 435
MS.gene35962.t1 MTR_7g076150 45.534 459 222 8 154 600 2 444 6.21e-139 412
MS.gene35962.t1 MTR_5g080720 43.061 490 248 12 63 534 98 574 1.45e-135 408
MS.gene35962.t1 MTR_5g026930 42.913 508 261 10 9 508 13 499 5.76e-133 399
MS.gene35962.t1 MTR_3g096480 41.712 549 280 18 73 600 247 776 6.45e-132 405
MS.gene35962.t1 MTR_5g083150 39.388 523 279 13 92 600 100 598 2.02e-130 395
MS.gene35962.t1 MTR_3g071530 38.803 518 285 11 92 600 152 646 9.18e-127 387
MS.gene35962.t1 MTR_8g093880 41.292 511 267 14 93 584 298 794 1.30e-126 392
MS.gene35962.t1 MTR_8g076650 41.811 519 264 15 93 591 387 887 1.40e-125 392
MS.gene35962.t1 MTR_5g021100 40.406 542 282 16 75 591 305 830 1.52e-125 390
MS.gene35962.t1 MTR_1g051055 38.795 531 292 12 87 600 89 603 1.18e-122 375
MS.gene35962.t1 MTR_3g088790 40.076 529 279 17 92 600 232 742 2.08e-121 377
MS.gene35962.t1 MTR_4g083030 32.065 552 333 11 70 599 72 603 2.62e-93 299
MS.gene35962.t1 MTR_1g022255 33.026 542 312 16 76 600 192 699 1.31e-90 295
MS.gene35962.t1 MTR_3g074780 30.648 571 344 14 70 605 140 693 1.31e-89 292
MS.gene35962.t1 MTR_5g064080 30.212 566 333 14 70 600 132 670 2.05e-84 278
MS.gene35962.t1 MTR_1g022255 28.186 408 248 12 76 467 192 570 2.71e-45 169
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35962.t1 AT1G26850 80.163 615 113 3 3 610 4 616 0.0 1057
MS.gene35962.t1 AT1G26850 80.163 615 113 3 3 610 4 616 0.0 1057
MS.gene35962.t1 AT4G18030 68.272 602 180 4 7 602 9 605 0.0 881
MS.gene35962.t1 AT1G26850 79.000 500 98 2 3 495 4 503 0.0 851
MS.gene35962.t1 AT4G10440 55.122 615 247 5 7 601 12 617 0.0 727
MS.gene35962.t1 AT1G33170 53.155 634 253 6 5 601 9 635 0.0 713
MS.gene35962.t1 AT2G45750 54.058 616 253 9 17 608 19 628 0.0 684
MS.gene35962.t1 AT4G00750 51.809 608 279 4 16 609 24 631 0.0 677
MS.gene35962.t1 AT2G43200 52.031 517 242 4 84 595 91 606 0.0 567
MS.gene35962.t1 AT2G43200 52.031 517 242 4 84 595 91 606 0.0 567
MS.gene35962.t1 AT1G31850 42.114 615 328 14 2 607 8 603 7.67e-173 504
MS.gene35962.t1 AT1G31850 42.114 615 328 14 2 607 8 603 7.67e-173 504
MS.gene35962.t1 AT1G31850 42.114 615 328 14 2 607 8 603 7.67e-173 504
MS.gene35962.t1 AT1G31850 42.114 615 328 14 2 607 8 603 7.67e-173 504
MS.gene35962.t1 AT4G19120 43.942 553 295 9 69 610 52 600 2.43e-169 495
MS.gene35962.t1 AT4G19120 43.942 553 295 9 69 610 52 600 2.43e-169 495
MS.gene35962.t1 AT4G00740 43.049 597 302 11 22 606 29 599 9.25e-165 484
MS.gene35962.t1 AT4G00740 43.049 597 302 11 22 606 29 599 9.25e-165 484
MS.gene35962.t1 AT5G06050 44.090 533 271 12 80 599 145 663 4.10e-150 449
MS.gene35962.t1 AT1G04430 41.799 567 287 12 64 602 64 615 1.49e-145 435
MS.gene35962.t1 AT1G04430 41.799 567 287 12 64 602 64 615 1.49e-145 435
MS.gene35962.t1 AT1G04430 41.799 567 287 12 64 602 64 615 1.49e-145 435
MS.gene35962.t1 AT2G39750 41.423 548 292 11 69 598 154 690 1.52e-145 437
MS.gene35962.t1 AT4G14360 43.444 511 259 10 82 575 74 571 3.44e-143 429
MS.gene35962.t1 AT4G14360 43.444 511 259 10 82 575 74 571 3.44e-143 429
MS.gene35962.t1 AT3G23300 42.329 541 278 12 82 602 77 603 8.57e-143 427
MS.gene35962.t1 AT3G23300 42.329 541 278 12 82 602 77 603 8.57e-143 427
MS.gene35962.t1 AT1G77260 40.418 574 304 14 48 598 96 654 2.26e-140 423
MS.gene35962.t1 AT4G19120 44.789 451 235 8 69 508 52 499 3.53e-139 414
MS.gene35962.t1 AT5G14430 39.234 627 331 16 10 608 8 612 9.29e-137 412
MS.gene35962.t1 AT5G14430 38.915 627 333 16 10 608 8 612 3.70e-135 408
MS.gene35962.t1 AT2G34300 41.714 525 272 13 93 600 250 757 8.75e-130 399
MS.gene35962.t1 AT2G34300 41.714 525 272 13 93 600 250 757 8.75e-130 399
MS.gene35962.t1 AT2G34300 41.714 525 272 13 93 600 250 757 8.75e-130 399
MS.gene35962.t1 AT5G04060 40.076 529 285 11 87 600 86 597 1.38e-129 393
MS.gene35962.t1 AT1G29470 41.714 525 272 14 93 600 250 757 5.32e-129 397
MS.gene35962.t1 AT1G29470 41.714 525 272 14 93 600 250 757 5.32e-129 397
MS.gene35962.t1 AT5G64030 42.095 506 262 14 93 580 308 800 5.71e-129 399
MS.gene35962.t1 AT3G10200 39.474 532 286 13 87 600 75 588 4.73e-127 387
MS.gene35962.t1 AT3G56080 38.921 519 282 11 93 600 114 608 2.85e-126 385
MS.gene35962.t1 AT3G51070 39.798 593 302 20 36 600 324 889 3.63e-126 393
MS.gene35962.t1 AT2G40280 37.524 525 286 12 89 600 86 581 6.41e-126 384
MS.gene35962.t1 AT3G10200 38.921 519 275 14 74 573 67 562 3.92e-118 363
MS.gene35962.t1 AT3G56080 40.625 448 239 10 93 529 114 545 2.43e-112 347
MS.gene35962.t1 AT3G51070 40.962 520 260 18 36 529 324 822 4.24e-109 347
MS.gene35962.t1 AT1G13860 34.522 533 297 14 82 585 79 588 1.74e-95 305
MS.gene35962.t1 AT1G13860 34.522 533 297 14 82 585 79 588 1.74e-95 305
MS.gene35962.t1 AT1G13860 34.522 533 297 14 82 585 79 588 1.74e-95 305
MS.gene35962.t1 AT1G13860 34.522 533 297 14 82 585 79 588 1.74e-95 305
MS.gene35962.t1 AT2G03480 30.990 626 347 16 10 599 16 592 1.17e-91 295
MS.gene35962.t1 AT2G03480 31.200 625 358 16 10 599 16 603 9.61e-91 293
MS.gene35962.t1 AT1G13860 37.188 441 239 12 171 585 4 432 5.21e-88 281
MS.gene35962.t1 AT1G19430 31.955 532 317 15 84 600 221 722 1.51e-85 282
MS.gene35962.t1 AT1G19430 31.955 532 317 15 84 600 221 722 1.51e-85 282
MS.gene35962.t1 AT1G78240 30.742 566 337 14 70 599 132 678 3.44e-83 275
MS.gene35962.t1 AT1G78240 30.742 566 337 14 70 599 132 678 3.44e-83 275
MS.gene35962.t1 AT2G03480 31.461 356 219 7 57 395 52 399 4.06e-50 179
MS.gene35962.t1 AT1G19430 27.960 397 247 12 87 469 224 595 4.23e-43 164

Find 152 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTTATTGGGTGCTTTC+TGG 0.079747 8.2:-41494420 MS.gene35962:CDS
TGCTGTTTCTTCTATATATT+GGG 0.197614 8.2:-41495726 MS.gene35962:CDS
TGTGAATCAAAACTTAAATT+TGG 0.201442 8.2:+41495621 None:intergenic
GTGCTGTTTCTTCTATATAT+TGG 0.235126 8.2:-41495727 MS.gene35962:CDS
ACTCCGACTCCTAAAGTTTC+TGG 0.236262 8.2:-41493776 MS.gene35962:CDS
TTCTAAGGCCTGGTGGTTAT+TGG 0.265507 8.2:-41494431 MS.gene35962:CDS
CAAGTTGGGGTGCATATCTT+TGG 0.273541 8.2:-41494772 MS.gene35962:CDS
GTTTCTAGTGGTTCTATTCC+TGG 0.277975 8.2:-41493704 MS.gene35962:CDS
TCTAAGGCCTGGTGGTTATT+GGG 0.298345 8.2:-41494430 MS.gene35962:CDS
CGGAAGATATCGCAACATTA+TGG 0.305436 8.2:-41493594 MS.gene35962:CDS
AGATCGCCGCCAGAAACTTT+AGG 0.311063 8.2:+41493767 None:intergenic
TGAAGTCGATGTGTTAATTA+AGG 0.331599 8.2:-41492416 MS.gene35962:CDS
CGCCTTCTGGTCGCAATATT+CGG 0.333406 8.2:+41492455 None:intergenic
AATTGCTGTCAAACAGTATT+GGG 0.341304 8.2:-41492311 MS.gene35962:CDS
AACCAAGTGCTGCTGATAAT+AGG 0.347469 8.2:-41495788 MS.gene35962:CDS
CATATGTCAGGTTGCAAGTT+GGG 0.349075 8.2:-41494786 MS.gene35962:intron
ATTAATTCACTGTTGGATTC+CGG 0.352872 8.2:-41493614 MS.gene35962:CDS
AGAAGGCTGAAATTGCAATT+TGG 0.354585 8.2:-41494278 MS.gene35962:CDS
GTGCAGATCTTTATAGTAGT+TGG 0.359407 8.2:-41495753 MS.gene35962:CDS
GTTCTCGCTGTTTGATTCCA+TGG 0.364915 8.2:-41494598 MS.gene35962:CDS
ATTAAGGTAAAGAAGTTAAT+CGG 0.370727 8.2:-41492400 MS.gene35962:CDS
GCATGGCAAAGAAGTGGTTT+TGG 0.370846 8.2:-41495702 MS.gene35962:CDS
TAGTTAACCTTCCAATTGAT+TGG 0.377261 8.2:+41494393 None:intergenic
CGGATTATGACAGCGCCTTC+TGG 0.378229 8.2:+41492442 None:intergenic
AAAGGTGTTACATACCCTTT+TGG 0.379736 8.2:+41495402 None:intergenic
ATCTTAAACCATTTCCAAAC+AGG 0.389098 8.2:-41493748 MS.gene35962:CDS
TATGGATATGAATGCTGGTT+TGG 0.391975 8.2:-41493576 MS.gene35962:CDS
GGGACATAATCCCTACTCTT+AGG 0.394894 8.2:+41495372 None:intergenic
TCCTCCTTTGGTCTCTGCCA+TGG 0.397409 8.2:+41494366 None:intergenic
TACGATCTCATTCATGCTAA+TGG 0.398294 8.2:-41493290 MS.gene35962:CDS
TTGAGAAGGCTATTCAGAAT+TGG 0.398684 8.2:-41495317 MS.gene35962:CDS
AATGCGTACCAGACATCATC+AGG 0.401430 8.2:+41494180 None:intergenic
AATGAGATGGAATATGAAAT+TGG 0.407077 8.2:-41492374 MS.gene35962:CDS
TTCTCGCTGTTTGATTCCAT+GGG 0.411610 8.2:-41494597 MS.gene35962:CDS
AGGGGTGTACCTGCTGTTAT+TGG 0.412993 8.2:-41494677 MS.gene35962:CDS
GCATATAGCCTGTTTGGAAA+TGG 0.416366 8.2:+41493740 None:intergenic
TTGTAAGGTGCATTAGCATA+AGG 0.420580 8.2:+41495351 None:intergenic
GGGATCGCATATAGCCTGTT+TGG 0.420901 8.2:+41493734 None:intergenic
CAAGAAAATTAATTCACTGT+TGG 0.428445 8.2:-41493621 MS.gene35962:CDS
TGCCATGTCGTTTGCACCGA+GGG 0.428690 8.2:-41494735 MS.gene35962:CDS
TGAATGAGATCGTATGTTCT+TGG 0.433817 8.2:+41493299 None:intergenic
TCTTCCTCGAGTTCCTCCTT+TGG 0.435137 8.2:+41494354 None:intergenic
ACAGCAATTAGTACTTTCTC+AGG 0.435226 8.2:+41492325 None:intergenic
AAACACCTTAGTGTTCGAAT+TGG 0.435639 8.2:+41495566 None:intergenic
CAATGTAACTTCTCTTCCTC+TGG 0.435812 8.2:+41495438 None:intergenic
TCAGAATTGGATCCAATATG+AGG 0.436848 8.2:-41495304 MS.gene35962:CDS
AGGTTGCTAAGAAACTTTGC+TGG 0.437795 8.2:-41494314 MS.gene35962:CDS
TAATTGCTGTCAAACAGTAT+TGG 0.437862 8.2:-41492312 MS.gene35962:CDS
ATCTTGACACGGAGTGTAAT+CGG 0.437871 8.2:+41495521 None:intergenic
CATAATGTTGCGATATCTTC+CGG 0.443123 8.2:+41493595 None:intergenic
ATGGATATGAATGCTGGTTT+GGG 0.443758 8.2:-41493575 MS.gene35962:CDS
ACAGCACTTGACACCGGCTG+TGG 0.452522 8.2:-41495174 MS.gene35962:CDS
CTCACTGTCCCATCATTGAT+AGG 0.455381 8.2:+41495198 None:intergenic
TTCTTACCATTTGCTCCCCA+TGG 0.455409 8.2:+41494581 None:intergenic
TGGAAGTTGATAGAGTTCTA+AGG 0.464058 8.2:-41494446 MS.gene35962:CDS
AACATTATGGATATGAATGC+TGG 0.465021 8.2:-41493581 MS.gene35962:CDS
GCCATGTCGAAGGCTCTTGA+TGG 0.465360 8.2:+41494626 None:intergenic
TTGATTCCATGGGGAGCAAA+TGG 0.467921 8.2:-41494587 MS.gene35962:intron
TCTGTTATTCCTATCAATGA+TGG 0.468006 8.2:-41495207 MS.gene35962:CDS
TTTCTGGCCCTCCAATCAAT+TGG 0.470413 8.2:-41494404 MS.gene35962:CDS
TGTATGATCCCAGCACCAAA+AGG 0.471853 8.2:-41495417 MS.gene35962:CDS
AAGTACAATTTGCTCTAGAA+AGG 0.476126 8.2:-41494697 MS.gene35962:CDS
AGTACAATTTGCTCTAGAAA+GGG 0.480566 8.2:-41494696 MS.gene35962:CDS
GGAAATTGTGTTCCACCACC+AGG 0.481645 8.2:+41495264 None:intergenic
TAACCTTCCAATTGATTGGA+GGG 0.491146 8.2:+41494397 None:intergenic
AAAAGGAGATAGTATAGCAT+TGG 0.491210 8.2:-41495679 MS.gene35962:CDS
CCTCAGACCCTGATGATGTC+TGG 0.492816 8.2:-41494188 MS.gene35962:intron
TGTAAGGTGCATTAGCATAA+GGG 0.498151 8.2:+41495352 None:intergenic
GTCCTATTATCAGCAGCACT+TGG 0.509665 8.2:+41495786 None:intergenic
TGATTGGCATCTATCATGAT+TGG 0.510283 8.2:-41493451 MS.gene35962:intron
TCTTCTATATATTGGGAGCA+TGG 0.513373 8.2:-41495719 MS.gene35962:CDS
TGGCCTCTTTAGTTTGTACC+AGG 0.518458 8.2:-41493270 MS.gene35962:CDS
ATATATGAGCGAGGACTGAT+TGG 0.519909 8.2:-41493467 MS.gene35962:CDS
TCGAAGGCTCTTGATGGGTA+TGG 0.523510 8.2:+41494632 None:intergenic
CAGCACTTGACACCGGCTGT+GGG 0.529046 8.2:-41495173 MS.gene35962:CDS
CTACTCTTAGGCCATGGAAA+AGG 0.529226 8.2:+41495384 None:intergenic
TATGTCTCAGAAGAAACTCC+AGG 0.532169 8.2:+41493686 None:intergenic
CTATTCATTCATCTAAGTCA+TGG 0.534410 8.2:-41493538 MS.gene35962:CDS
TATCCTGGTACAAACTAAAG+AGG 0.534977 8.2:+41493267 None:intergenic
TGGCCCTCCAATCAATTGGA+AGG 0.541843 8.2:-41494400 MS.gene35962:CDS
TCATATGTCAGGTTGCAAGT+TGG 0.542053 8.2:-41494787 MS.gene35962:intron
CAAGAGTCTCACAGTTGAGA+AGG 0.546705 8.2:-41495331 MS.gene35962:CDS
GAAGGAACACTTACTTATCC+TGG 0.549922 8.2:+41493252 None:intergenic
ATATGTCAGGTTGCAAGTTG+GGG 0.550068 8.2:-41494785 MS.gene35962:intron
AAACAGTATTGGGTTACTGA+TGG 0.550680 8.2:-41492301 MS.gene35962:CDS
CAGTGATTATTGTGTTGATG+TGG 0.551077 8.2:+41492273 None:intergenic
TCAACTGTGAGACTCTTGTA+AGG 0.555125 8.2:+41495336 None:intergenic
AGTTTCTTCTGAGACATACC+AGG 0.557089 8.2:-41493681 MS.gene35962:CDS
GTTGATAGAGTTCTAAGGCC+TGG 0.557635 8.2:-41494441 MS.gene35962:CDS
CTGTTATTCCTATCAATGAT+GGG 0.558379 8.2:-41495206 MS.gene35962:CDS
TATTCATTCATCTAAGTCAT+GGG 0.558548 8.2:-41493537 MS.gene35962:CDS
TGAATCCAATTCGAACACTA+AGG 0.559388 8.2:-41495571 MS.gene35962:CDS
ATCGTATGTTCTTGGATATG+TGG 0.561019 8.2:+41493307 None:intergenic
TTAACCTTCCAATTGATTGG+AGG 0.563633 8.2:+41494396 None:intergenic
AGAACAAAGAAAGATTGAAG+AGG 0.565209 8.2:-41494334 MS.gene35962:CDS
AGTTAATCGGAGGAATGAGA+TGG 0.569106 8.2:-41492387 MS.gene35962:CDS
CAGAATTGGATCCAATATGA+GGG 0.569553 8.2:-41495303 MS.gene35962:CDS
TGTGCTTCGTGAGAGTCCCT+CGG 0.570141 8.2:+41494719 None:intergenic
ATAGAACCACTAGAAACTCT+AGG 0.570235 8.2:+41493710 None:intergenic
TAATCCCTACTCTTAGGCCA+TGG 0.572458 8.2:+41495378 None:intergenic
AAATTGGTCGATCATGAAGA+TGG 0.576257 8.2:-41492358 MS.gene35962:CDS
CCAGACATCATCAGGGTCTG+AGG 0.577463 8.2:+41494188 None:intergenic
GGTTGCTAAGAAACTTTGCT+GGG 0.577899 8.2:-41494313 MS.gene35962:CDS
CCATGTCGAAGGCTCTTGAT+GGG 0.579790 8.2:+41494627 None:intergenic
CTGGGAGAAGAAGTCTGAGA+AGG 0.587700 8.2:-41494295 MS.gene35962:CDS
AGAAGACATTCTTCTCGAGA+TGG 0.592742 8.2:-41492479 MS.gene35962:CDS
AGTACTTTCTCAGGAACAAG+AGG 0.593177 8.2:+41492334 None:intergenic
GTATGATCCCAGCACCAAAA+GGG 0.596741 8.2:-41495416 MS.gene35962:CDS
TTGGGAGCATGGCAAAGAAG+TGG 0.598147 8.2:-41495708 MS.gene35962:CDS
ACCAGGATGATAACAAAATG+TGG 0.598954 8.2:-41493664 MS.gene35962:CDS
GACCGAATATTGCGACCAGA+AGG 0.601380 8.2:-41492457 MS.gene35962:CDS
ACATAAACATACCCCACAGC+CGG 0.601588 8.2:+41495161 None:intergenic
GTCCCTCGGTGCAAACGACA+TGG 0.602825 8.2:+41494733 None:intergenic
CCGCCAGAAACTTTAGGAGT+CGG 0.604385 8.2:+41493773 None:intergenic
CTATAGGTATAAGAAAATGA+AGG 0.604922 8.2:-41493807 MS.gene35962:intron
TGCAGATGGAATGTATATGA+TGG 0.612639 8.2:-41494466 MS.gene35962:intron
TTTGACTAACTTCACCAGCA+TGG 0.614335 8.2:+41495598 None:intergenic
GTGAGGACAGCACTTGACAC+CGG 0.622115 8.2:-41495180 MS.gene35962:CDS
AGAGAGGCACTGTCCTCCAG+AGG 0.624356 8.2:-41495454 MS.gene35962:CDS
GAGACCAAAGGAGGAACTCG+AGG 0.624522 8.2:-41494358 MS.gene35962:CDS
AAAACATGAACTATAGAGAG+AGG 0.627843 8.2:-41495470 MS.gene35962:CDS
GAACCACTAGAAACTCTAGG+AGG 0.628946 8.2:+41493713 None:intergenic
CCCATCAAGAGCCTTCGACA+TGG 0.629295 8.2:-41494627 MS.gene35962:CDS
ATCCCTCCTAGAGTTTCTAG+TGG 0.638071 8.2:-41493716 MS.gene35962:CDS
GGATTCGCAAAATTCTACAC+TGG 0.639914 8.2:+41494209 None:intergenic
AACCACTAGAAACTCTAGGA+GGG 0.640857 8.2:+41493714 None:intergenic
ACACTCCGTGTCAAGATCAA+CGG 0.641064 8.2:-41495515 MS.gene35962:CDS
GTACAATTTGCTCTAGAAAG+GGG 0.642156 8.2:-41494695 MS.gene35962:CDS
AGAACAATGAGCCATGTCGA+AGG 0.642478 8.2:+41494616 None:intergenic
GAAAGCACCCAATAACCACC+AGG 0.649776 8.2:+41494423 None:intergenic
GGAAGGTTAACTACAAACCA+TGG 0.649822 8.2:-41494383 MS.gene35962:CDS
GGTGGAACACAATTTCCTCA+AGG 0.651943 8.2:-41495258 MS.gene35962:CDS
TTGTCTGCTACGACATGACT+CGG 0.659816 8.2:+41494239 None:intergenic
ATATATTTATCAGCACCTTG+AGG 0.663185 8.2:+41495243 None:intergenic
TGTAACTTCTCTTCCTCTGG+AGG 0.663464 8.2:+41495441 None:intergenic
AAGGTAAAGAAGTTAATCGG+AGG 0.666069 8.2:-41492397 MS.gene35962:CDS
CAAACCATGGCAGAGACCAA+AGG 0.668045 8.2:-41494370 MS.gene35962:CDS
CCGACTCCTAAAGTTTCTGG+CGG 0.668444 8.2:-41493773 MS.gene35962:CDS
TTGCCATGTCGTTTGCACCG+AGG 0.669899 8.2:-41494736 MS.gene35962:CDS
TCTCGCTGTTTGATTCCATG+GGG 0.671713 8.2:-41494596 MS.gene35962:CDS
GAAATTGTGTTCCACCACCA+GGG 0.674923 8.2:+41495265 None:intergenic
ACCATGGCAGAGACCAAAGG+AGG 0.677739 8.2:-41494367 MS.gene35962:CDS
TCGGTATAACGAGCATCACA+AGG 0.677771 8.2:+41495540 None:intergenic
GATAGAGTTCTAAGGCCTGG+TGG 0.678122 8.2:-41494438 MS.gene35962:CDS
AGCACTTGACACCGGCTGTG+GGG 0.680746 8.2:-41495172 MS.gene35962:intron
TGACTTAGATGAATGAATAG+CGG 0.692625 8.2:+41493541 None:intergenic
ATATCTTTGGAGCAGAAATG+TGG 0.695730 8.2:-41494759 MS.gene35962:CDS
CTCGGTGTGATATATGAGCG+AGG 0.704433 8.2:-41493476 MS.gene35962:CDS
ATGCGTACCAGACATCATCA+GGG 0.712734 8.2:+41494181 None:intergenic
CTATCAATGATGGGACAGTG+AGG 0.716456 8.2:-41495197 MS.gene35962:CDS
GCACGCCGTTGATCTTGACA+CGG 0.740888 8.2:+41495510 None:intergenic
TTAACACATCGACTTCATCG+CGG 0.761114 8.2:+41492422 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATATAACAACAAAAA+GGG + chr8.2:41493927-41493946 None:intergenic 10.0%
!! TTTATTATATAACAACAAAA+AGG + chr8.2:41493928-41493947 None:intergenic 10.0%
!!! TTTTTCTTATATATTTTTGT+TGG - chr8.2:41494718-41494737 MS.gene35962:CDS 10.0%
!! AATATATAAGAAAAAAGAAC+AGG + chr8.2:41494713-41494732 None:intergenic 15.0%
!! ATAAAAACATATAGCTTATA+TGG + chr8.2:41493069-41493088 None:intergenic 15.0%
!! TATTATATAACAACAAAAAG+GGG + chr8.2:41493926-41493945 None:intergenic 15.0%
!!! TGCACATTTTAATAAAAATT+AGG - chr8.2:41495155-41495174 MS.gene35962:intron 15.0%
!!! TTATCTTTATTGTTTTATGT+TGG + chr8.2:41495282-41495301 None:intergenic 15.0%
!!! TTTTATAATGACCATATTTA+GGG + chr8.2:41495035-41495054 None:intergenic 15.0%
!!! TTTTTATAATGACCATATTT+AGG + chr8.2:41495036-41495055 None:intergenic 15.0%
!! AAAGTCATACAATCAATATA+AGG - chr8.2:41495367-41495386 MS.gene35962:CDS 20.0%
!! ATTAAGGTAAAGAAGTTAAT+CGG - chr8.2:41495674-41495693 MS.gene35962:CDS 20.0%
!! ATTATATAACAACAAAAAGG+GGG + chr8.2:41493925-41493944 None:intergenic 20.0%
!! TAATGTTGTATAGAAATAAG+AGG - chr8.2:41494213-41494232 MS.gene35962:CDS 20.0%
!! TACATATTACTTTGACTATT+CGG - chr8.2:41493234-41493253 MS.gene35962:intron 20.0%
!! TGTGAATCAAAACTTAAATT+TGG + chr8.2:41492456-41492475 None:intergenic 20.0%
!!! CACATTTTTTTCTTCAATTT+TGG - chr8.2:41495187-41495206 MS.gene35962:CDS 20.0%
!!! CTATATGTTTTTATAAGCTA+TGG - chr8.2:41493075-41493094 MS.gene35962:intron 20.0%
! AATGAGATGGAATATGAAAT+TGG - chr8.2:41495700-41495719 MS.gene35962:CDS 25.0%
! AATGTATGTATTCATATGTC+AGG - chr8.2:41493276-41493295 MS.gene35962:CDS 25.0%
! AGATCTGACATGAATAAAAT+AGG + chr8.2:41494671-41494690 None:intergenic 25.0%
! CAAGAAAATTAATTCACTGT+TGG - chr8.2:41494453-41494472 MS.gene35962:CDS 25.0%
! CTATAGGTATAAGAAAATGA+AGG - chr8.2:41494267-41494286 MS.gene35962:CDS 25.0%
! GAGAACTTAAAAGAAATAAG+CGG - chr8.2:41493100-41493119 MS.gene35962:intron 25.0%
! TAAAAACATTTCCCTCATAT+TGG + chr8.2:41492785-41492804 None:intergenic 25.0%
! TAAAATAGGATCGAATAGTT+AGG + chr8.2:41494657-41494676 None:intergenic 25.0%
! TATTCATTCATCTAAGTCAT+GGG - chr8.2:41494537-41494556 MS.gene35962:intron 25.0%
! TTATATAACAACAAAAAGGG+GGG + chr8.2:41493924-41493943 None:intergenic 25.0%
!! AAAAACAAGTTTTCTGCTAA+TGG + chr8.2:41495492-41495511 None:intergenic 25.0%
!! CACGATTTGACATTATATTT+TGG - chr8.2:41493576-41493595 MS.gene35962:CDS 25.0%
!!! ATAGTTCTAAATTGCTATTG+CGG - chr8.2:41494012-41494031 MS.gene35962:intron 25.0%
!!! CTCATTTTTCTGCAATATAA+AGG + chr8.2:41494049-41494068 None:intergenic 25.0%
!!! TGCTGTTTCTTCTATATATT+GGG - chr8.2:41492348-41492367 MS.gene35962:CDS 25.0%
!!! TTTTTGTTGGTTTTTTATCC+AGG - chr8.2:41494731-41494750 MS.gene35962:CDS 25.0%
AAAACATGAACTATAGAGAG+AGG - chr8.2:41492604-41492623 MS.gene35962:intron 30.0%
AAAAGGAGATAGTATAGCAT+TGG - chr8.2:41492395-41492414 MS.gene35962:CDS 30.0%
AACATTATGGATATGAATGC+TGG - chr8.2:41494493-41494512 MS.gene35962:intron 30.0%
AATTGCTGTCAAACAGTATT+GGG - chr8.2:41495763-41495782 MS.gene35962:CDS 30.0%
AGAACAAAGAAAGATTGAAG+AGG - chr8.2:41493740-41493759 MS.gene35962:CDS 30.0%
ATATATTTATCAGCACCTTG+AGG + chr8.2:41492834-41492853 None:intergenic 30.0%
ATCTTAAACCATTTCCAAAC+AGG - chr8.2:41494326-41494345 MS.gene35962:CDS 30.0%
ATTAAGCTGTTTAACCAAAC+AGG + chr8.2:41492986-41493005 None:intergenic 30.0%
ATTAATTCACTGTTGGATTC+CGG - chr8.2:41494460-41494479 MS.gene35962:CDS 30.0%
CTATTCATTCATCTAAGTCA+TGG - chr8.2:41494536-41494555 MS.gene35962:intron 30.0%
CTGTTATTCCTATCAATGAT+GGG - chr8.2:41492868-41492887 MS.gene35962:intron 30.0%
GCTTATATGGAAAAACACTT+TGG + chr8.2:41493056-41493075 None:intergenic 30.0%
TAATTGCTGTCAAACAGTAT+TGG - chr8.2:41495762-41495781 MS.gene35962:CDS 30.0%
TAGTTAACCTTCCAATTGAT+TGG + chr8.2:41493684-41493703 None:intergenic 30.0%
TCTGTTATTCCTATCAATGA+TGG - chr8.2:41492867-41492886 MS.gene35962:intron 30.0%
TGAAGTCGATGTGTTAATTA+AGG - chr8.2:41495658-41495677 MS.gene35962:CDS 30.0%
TGACTTAGATGAATGAATAG+CGG + chr8.2:41494536-41494555 None:intergenic 30.0%
TTAAATAGTCATTTGACCGA+AGG + chr8.2:41494843-41494862 None:intergenic 30.0%
TTAAGCTGTTTAACCAAACA+GGG + chr8.2:41492985-41493004 None:intergenic 30.0%
TTCTTTCGAAGCAGATAAAA+TGG - chr8.2:41495318-41495337 MS.gene35962:CDS 30.0%
! ATAGCTGAAAAAAGTACACT+CGG - chr8.2:41494580-41494599 MS.gene35962:intron 30.0%
! CTATAGTTCATGTTTTCTCT+CGG + chr8.2:41492600-41492619 None:intergenic 30.0%
! GCTAATTTGAGTTTATCTAC+TGG + chr8.2:41493195-41493214 None:intergenic 30.0%
!! AAGTACAATTTGCTCTAGAA+AGG - chr8.2:41493377-41493396 MS.gene35962:intron 30.0%
!! AGTACAATTTGCTCTAGAAA+GGG - chr8.2:41493378-41493397 MS.gene35962:intron 30.0%
!! GTGCTGTTTCTTCTATATAT+TGG - chr8.2:41492347-41492366 MS.gene35962:CDS 30.0%
!! TGTAGGTTTTAACGTAAGAT+GGG + chr8.2:41495112-41495131 None:intergenic 30.0%
!! TTGCAGCTAATAGTTTTACA+TGG + chr8.2:41493547-41493566 None:intergenic 30.0%
!!! GTACTTTTTTCAGCTATAGT+AGG + chr8.2:41494577-41494596 None:intergenic 30.0%
AAACACCTTAGTGTTCGAAT+TGG + chr8.2:41492511-41492530 None:intergenic 35.0%
AAATTAGCCACAATCCAAAC+TGG - chr8.2:41493207-41493226 MS.gene35962:intron 35.0%
AAATTGGTCGATCATGAAGA+TGG - chr8.2:41495716-41495735 MS.gene35962:CDS 35.0%
AACTTCACACAGTATTGCAT+TGG - chr8.2:41494919-41494938 MS.gene35962:intron 35.0%
AAGGTAAAGAAGTTAATCGG+AGG - chr8.2:41495677-41495696 MS.gene35962:CDS 35.0%
AATGCAATACTGTGTGAAGT+TGG + chr8.2:41494920-41494939 None:intergenic 35.0%
ACAGCAATTAGTACTTTCTC+AGG + chr8.2:41495752-41495771 None:intergenic 35.0%
ACCAGGATGATAACAAAATG+TGG - chr8.2:41494410-41494429 MS.gene35962:CDS 35.0%
ACCATATTTAGGGACTGAAA+TGG + chr8.2:41495025-41495044 None:intergenic 35.0%
ACTTCACACAGTATTGCATT+GGG - chr8.2:41494920-41494939 MS.gene35962:intron 35.0%
AGAAGGCTGAAATTGCAATT+TGG - chr8.2:41493796-41493815 MS.gene35962:CDS 35.0%
AGGATAAGTAAGTGTTCCTT+CGG - chr8.2:41494824-41494843 MS.gene35962:intron 35.0%
ATAGAACCACTAGAAACTCT+AGG + chr8.2:41494367-41494386 None:intergenic 35.0%
ATCGTATGTTCTTGGATATG+TGG + chr8.2:41494770-41494789 None:intergenic 35.0%
CAGAATTGGATCCAATATGA+GGG - chr8.2:41492771-41492790 MS.gene35962:intron 35.0%
CATAATGTTGCGATATCTTC+CGG + chr8.2:41494482-41494501 None:intergenic 35.0%
GAGGTTTAACAAAACTGATC+AGG + chr8.2:41492957-41492976 None:intergenic 35.0%
GTAATATGTACAAGCCAGTT+TGG + chr8.2:41493224-41493243 None:intergenic 35.0%
GTATGTTCCTATGACAAGTA+GGG + chr8.2:41493981-41494000 None:intergenic 35.0%
GTGCAGATCTTTATAGTAGT+TGG - chr8.2:41492321-41492340 MS.gene35962:CDS 35.0%
GTTTGTAGACTCAACAAACA+AGG + chr8.2:41494957-41494976 None:intergenic 35.0%
TAACCTTCCAATTGATTGGA+GGG + chr8.2:41493680-41493699 None:intergenic 35.0%
TAAGCCAGTTCTTATTCTGT+AGG - chr8.2:41494885-41494904 MS.gene35962:intron 35.0%
TACGATCTCATTCATGCTAA+TGG - chr8.2:41494784-41494803 MS.gene35962:intron 35.0%
TATCCTGGTACAAACTAAAG+AGG + chr8.2:41494810-41494829 None:intergenic 35.0%
TCAGAATTGGATCCAATATG+AGG - chr8.2:41492770-41492789 MS.gene35962:intron 35.0%
TCCATTTCAGTCCCTAAATA+TGG - chr8.2:41495021-41495040 MS.gene35962:intron 35.0%
TCTTCTATATATTGGGAGCA+TGG - chr8.2:41492355-41492374 MS.gene35962:CDS 35.0%
TGAATCCAATTCGAACACTA+AGG - chr8.2:41492503-41492522 MS.gene35962:intron 35.0%
TGAATGAGATCGTATGTTCT+TGG + chr8.2:41494778-41494797 None:intergenic 35.0%
TGAGTCTACAAACTGTTACA+TGG - chr8.2:41494964-41494983 MS.gene35962:intron 35.0%
TGATTGGCATCTATCATGAT+TGG - chr8.2:41494623-41494642 MS.gene35962:CDS 35.0%
TGCAGATGGAATGTATATGA+TGG - chr8.2:41493608-41493627 MS.gene35962:CDS 35.0%
TGGTATGCAAAATCAAGACA+CGG + chr8.2:41495472-41495491 None:intergenic 35.0%
TGTAAGGTGCATTAGCATAA+GGG + chr8.2:41492725-41492744 None:intergenic 35.0%
TGTATGTTCCTATGACAAGT+AGG + chr8.2:41493982-41494001 None:intergenic 35.0%
TTAACCTTCCAATTGATTGG+AGG + chr8.2:41493681-41493700 None:intergenic 35.0%
TTGAGAAGGCTATTCAGAAT+TGG - chr8.2:41492757-41492776 MS.gene35962:intron 35.0%
TTGTAAGGTGCATTAGCATA+AGG + chr8.2:41492726-41492745 None:intergenic 35.0%
! AAACAGTATTGGGTTACTGA+TGG - chr8.2:41495773-41495792 MS.gene35962:CDS 35.0%
! AGAGTAGTTCAAGTTTCTTC+TGG + chr8.2:41495084-41495103 None:intergenic 35.0%
! AGTAGTTCAAGTTTCTTCTG+GGG + chr8.2:41495082-41495101 None:intergenic 35.0%
! GAGTAGTTCAAGTTTCTTCT+GGG + chr8.2:41495083-41495102 None:intergenic 35.0%
! GATTCTTTTAGTCGTATGCT+CGG + chr8.2:41494092-41494111 None:intergenic 35.0%
! TATGGATATGAATGCTGGTT+TGG - chr8.2:41494498-41494517 MS.gene35962:intron 35.0%
!! ATATCTTTGGAGCAGAAATG+TGG - chr8.2:41493315-41493334 MS.gene35962:CDS 35.0%
!! ATGGATATGAATGCTGGTTT+GGG - chr8.2:41494499-41494518 MS.gene35962:intron 35.0%
!! GTACAATTTGCTCTAGAAAG+GGG - chr8.2:41493379-41493398 MS.gene35962:intron 35.0%
!! GTGTAGGTTTTAACGTAAGA+TGG + chr8.2:41495113-41495132 None:intergenic 35.0%
!! TCCACATTTTGTTATCATCC+TGG + chr8.2:41494414-41494433 None:intergenic 35.0%
!! TGATGGGTATGGCAATTTTA+TGG + chr8.2:41493434-41493453 None:intergenic 35.0%
!! TGGAAGTTGATAGAGTTCTA+AGG - chr8.2:41493628-41493647 MS.gene35962:CDS 35.0%
!! TTTGGTTGTTGATTTGCAGA+TGG - chr8.2:41493594-41493613 MS.gene35962:CDS 35.0%
!!! AAAGGTGTTACATACCCTTT+TGG + chr8.2:41492675-41492694 None:intergenic 35.0%
!!! AATGTTTTTAGATTCCCTGG+TGG - chr8.2:41492795-41492814 MS.gene35962:intron 35.0%
!!! CAAAGAAGTGGTTTTGGAAA+AGG - chr8.2:41492378-41492397 MS.gene35962:CDS 35.0%
!!! GGAAATGTTTTTAGATTCCC+TGG - chr8.2:41492792-41492811 MS.gene35962:intron 35.0%
!!! GTTTTTTATCCAGGTGTGAA+GGG - chr8.2:41494740-41494759 MS.gene35962:CDS 35.0%
!!! TCAGTTTTGTTAAACCTCCT+TGG - chr8.2:41492959-41492978 MS.gene35962:intron 35.0%
AGAAGACATTCTTCTCGAGA+TGG - chr8.2:41495595-41495614 MS.gene35962:CDS 40.0%
AGGTTGCTAAGAAACTTTGC+TGG - chr8.2:41493760-41493779 MS.gene35962:CDS 40.0%
AGTACTTTCTCAGGAACAAG+AGG + chr8.2:41495743-41495762 None:intergenic 40.0%
AGTTAATCGGAGGAATGAGA+TGG - chr8.2:41495687-41495706 MS.gene35962:CDS 40.0%
ATATATGAGCGAGGACTGAT+TGG - chr8.2:41494607-41494626 MS.gene35962:CDS 40.0%
ATCTTGACACGGAGTGTAAT+CGG + chr8.2:41492556-41492575 None:intergenic 40.0%
CAATGTAACTTCTCTTCCTC+TGG + chr8.2:41492639-41492658 None:intergenic 40.0%
CCAAAAACACCAATAACAGC+AGG + chr8.2:41493409-41493428 None:intergenic 40.0%
CGGAAGATATCGCAACATTA+TGG - chr8.2:41494480-41494499 MS.gene35962:intron 40.0%
CTTATCATCGAGAGTAGTGT+AGG + chr8.2:41495129-41495148 None:intergenic 40.0%
GAAGGAACACTTACTTATCC+TGG + chr8.2:41494825-41494844 None:intergenic 40.0%
GACAAGTAGGGAGAACTAAA+AGG + chr8.2:41493969-41493988 None:intergenic 40.0%
GCATATAGCCTGTTTGGAAA+TGG + chr8.2:41494337-41494356 None:intergenic 40.0%
GGAAGGTTAACTACAAACCA+TGG - chr8.2:41493691-41493710 MS.gene35962:CDS 40.0%
GGATTCGCAAAATTCTACAC+TGG + chr8.2:41493868-41493887 None:intergenic 40.0%
GGCATCTATCATGATTGGTA+TGG - chr8.2:41494628-41494647 MS.gene35962:CDS 40.0%
GGTTGCTAAGAAACTTTGCT+GGG - chr8.2:41493761-41493780 MS.gene35962:CDS 40.0%
TACGCCTACAGAATAAGAAC+TGG + chr8.2:41494892-41494911 None:intergenic 40.0%
TAGTTCTCCCTACTTGTCAT+AGG - chr8.2:41493971-41493990 MS.gene35962:intron 40.0%
TATGTCTCAGAAGAAACTCC+AGG + chr8.2:41494391-41494410 None:intergenic 40.0%
TCAACTGTGAGACTCTTGTA+AGG + chr8.2:41492741-41492760 None:intergenic 40.0%
TTCTCGCTGTTTGATTCCAT+GGG - chr8.2:41493477-41493496 MS.gene35962:CDS 40.0%
TTTGACTAACTTCACCAGCA+TGG + chr8.2:41492479-41492498 None:intergenic 40.0%
! AACCAAGTGCTGCTGATAAT+AGG - chr8.2:41492286-41492305 MS.gene35962:CDS 40.0%
! AACCACTAGAAACTCTAGGA+GGG + chr8.2:41494363-41494382 None:intergenic 40.0%
! ATATGTCAGGTTGCAAGTTG+GGG - chr8.2:41493289-41493308 MS.gene35962:CDS 40.0%
! CATATGTCAGGTTGCAAGTT+GGG - chr8.2:41493288-41493307 MS.gene35962:CDS 40.0%
! CTGGAAATGCTTTTGTTCTG+TGG - chr8.2:41494244-41494263 MS.gene35962:CDS 40.0%
! GAAATAAGAGGTGTACTGAC+TGG - chr8.2:41494225-41494244 MS.gene35962:CDS 40.0%
! GGTATGTAACACCTTTTCCA+TGG - chr8.2:41492679-41492698 MS.gene35962:intron 40.0%
! TCATATGTCAGGTTGCAAGT+TGG - chr8.2:41493287-41493306 MS.gene35962:CDS 40.0%
! TGCTTTTGTTCTGTGGCTAT+AGG - chr8.2:41494251-41494270 MS.gene35962:CDS 40.0%
! TTTTCCATGGCCTAAGAGTA+GGG - chr8.2:41492692-41492711 MS.gene35962:intron 40.0%
!! AGGTTTTAACGTAAGATGGG+AGG + chr8.2:41495109-41495128 None:intergenic 40.0%
!! AGTTTCTTCTGAGACATACC+AGG - chr8.2:41494393-41494412 MS.gene35962:CDS 40.0%
!! CCTGCTGTTATTGGTGTTTT+TGG - chr8.2:41493406-41493425 MS.gene35962:intron 40.0%
!! GGTTTTAACGTAAGATGGGA+GGG + chr8.2:41495108-41495127 None:intergenic 40.0%
!! GTTTCTAGTGGTTCTATTCC+TGG - chr8.2:41494370-41494389 MS.gene35962:CDS 40.0%
!! GTTTTAACGTAAGATGGGAG+GGG + chr8.2:41495107-41495126 None:intergenic 40.0%
!! TTAACACATCGACTTCATCG+CGG + chr8.2:41495655-41495674 None:intergenic 40.0%
!!! AGTTTTGATTCACACCATGC+TGG - chr8.2:41492462-41492481 MS.gene35962:CDS 40.0%
!!! GGTTTTTTATCCAGGTGTGA+AGG - chr8.2:41494739-41494758 MS.gene35962:CDS 40.0%
!!! TTACATACCCTTTTGGTGCT+GGG + chr8.2:41492668-41492687 None:intergenic 40.0%
AATGCGTACCAGACATCATC+AGG + chr8.2:41493897-41493916 None:intergenic 45.0%
ACACTCCGTGTCAAGATCAA+CGG - chr8.2:41492559-41492578 MS.gene35962:intron 45.0%
ACATAAACATACCCCACAGC+CGG + chr8.2:41492916-41492935 None:intergenic 45.0%
ACGCACTTGTGAGACTGTTT+GGG + chr8.2:41493169-41493188 None:intergenic 45.0%
ACTCCGACTCCTAAAGTTTC+TGG - chr8.2:41494298-41494317 MS.gene35962:CDS 45.0%
AGAACAATGAGCCATGTCGA+AGG + chr8.2:41493461-41493480 None:intergenic 45.0%
AGACTGTTTGGGAGAGCTTA+TGG + chr8.2:41493158-41493177 None:intergenic 45.0%
ATGCGTACCAGACATCATCA+GGG + chr8.2:41493896-41493915 None:intergenic 45.0%
CAAGAGTCTCACAGTTGAGA+AGG - chr8.2:41492743-41492762 MS.gene35962:intron 45.0%
CTACTCTTAGGCCATGGAAA+AGG + chr8.2:41492693-41492712 None:intergenic 45.0%
CTCACTGTCCCATCATTGAT+AGG + chr8.2:41492879-41492898 None:intergenic 45.0%
GAAATTGTGTTCCACCACCA+GGG + chr8.2:41492812-41492831 None:intergenic 45.0%
GAACCACTAGAAACTCTAGG+AGG + chr8.2:41494364-41494383 None:intergenic 45.0%
GGGACATAATCCCTACTCTT+AGG + chr8.2:41492705-41492724 None:intergenic 45.0%
GGTGGAACACAATTTCCTCA+AGG - chr8.2:41492816-41492835 MS.gene35962:intron 45.0%
GTACAAGCCAGTTTGGATTG+TGG + chr8.2:41493217-41493236 None:intergenic 45.0%
GTATGATCCCAGCACCAAAA+GGG - chr8.2:41492658-41492677 MS.gene35962:intron 45.0%
GTCCTATTATCAGCAGCACT+TGG + chr8.2:41492291-41492310 None:intergenic 45.0%
GTTCTCGCTGTTTGATTCCA+TGG - chr8.2:41493476-41493495 MS.gene35962:CDS 45.0%
TAATCCCTACTCTTAGGCCA+TGG + chr8.2:41492699-41492718 None:intergenic 45.0%
TACGCACTTGTGAGACTGTT+TGG + chr8.2:41493170-41493189 None:intergenic 45.0%
TCGGTATAACGAGCATCACA+AGG + chr8.2:41492537-41492556 None:intergenic 45.0%
TCTCGCTGTTTGATTCCATG+GGG - chr8.2:41493478-41493497 MS.gene35962:CDS 45.0%
TGTAACTTCTCTTCCTCTGG+AGG + chr8.2:41492636-41492655 None:intergenic 45.0%
TGTATGATCCCAGCACCAAA+AGG - chr8.2:41492657-41492676 MS.gene35962:intron 45.0%
TGTTTAACCAAACAGGGCCA+AGG + chr8.2:41492979-41492998 None:intergenic 45.0%
TTCTTACCATTTGCTCCCCA+TGG + chr8.2:41493496-41493515 None:intergenic 45.0%
TTGATTCCATGGGGAGCAAA+TGG - chr8.2:41493487-41493506 MS.gene35962:CDS 45.0%
TTGTCTGCTACGACATGACT+CGG + chr8.2:41493838-41493857 None:intergenic 45.0%
! ATCCCTCCTAGAGTTTCTAG+TGG - chr8.2:41494358-41494377 MS.gene35962:CDS 45.0%
! CAAGTTGGGGTGCATATCTT+TGG - chr8.2:41493302-41493321 MS.gene35962:CDS 45.0%
! CTATCAATGATGGGACAGTG+AGG - chr8.2:41492877-41492896 MS.gene35962:intron 45.0%
! CTTTTCCATGGCCTAAGAGT+AGG - chr8.2:41492691-41492710 MS.gene35962:intron 45.0%
! GCATGGCAAAGAAGTGGTTT+TGG - chr8.2:41492372-41492391 MS.gene35962:CDS 45.0%
! TCTAAGGCCTGGTGGTTATT+GGG - chr8.2:41493644-41493663 MS.gene35962:CDS 45.0%
! TTTCTGGCCCTCCAATCAAT+TGG - chr8.2:41493670-41493689 MS.gene35962:CDS 45.0%
!! GTTACATACCCTTTTGGTGC+TGG + chr8.2:41492669-41492688 None:intergenic 45.0%
!! GTTGATAGAGTTCTAAGGCC+TGG - chr8.2:41493633-41493652 MS.gene35962:CDS 45.0%
!! GTTTTTAGATTCCCTGGTGG+TGG - chr8.2:41492798-41492817 MS.gene35962:intron 45.0%
!! TGGCCTCTTTAGTTTGTACC+AGG - chr8.2:41494804-41494823 MS.gene35962:intron 45.0%
!! TTCAAGTTTCTTCTGGGGCT+GGG + chr8.2:41495077-41495096 None:intergenic 45.0%
!! TTCTAAGGCCTGGTGGTTAT+TGG - chr8.2:41493643-41493662 MS.gene35962:CDS 45.0%
AGATCGCCGCCAGAAACTTT+AGG + chr8.2:41494310-41494329 None:intergenic 50.0%
CAAACCATGGCAGAGACCAA+AGG - chr8.2:41493704-41493723 MS.gene35962:CDS 50.0%
CCATGTCGAAGGCTCTTGAT+GGG + chr8.2:41493450-41493469 None:intergenic 50.0%
CGCCTTCTGGTCGCAATATT+CGG + chr8.2:41495622-41495641 None:intergenic 50.0%
CTGGGAGAAGAAGTCTGAGA+AGG - chr8.2:41493779-41493798 MS.gene35962:CDS 50.0%
GACCGAATATTGCGACCAGA+AGG - chr8.2:41495617-41495636 MS.gene35962:CDS 50.0%
GGAAATTGTGTTCCACCACC+AGG + chr8.2:41492813-41492832 None:intergenic 50.0%
GGGATCGCATATAGCCTGTT+TGG + chr8.2:41494343-41494362 None:intergenic 50.0%
GGTGGTTATTGGGTGCTTTC+TGG - chr8.2:41493654-41493673 MS.gene35962:CDS 50.0%
TAAACCTCCTTGGCCCTGTT+TGG - chr8.2:41492969-41492988 MS.gene35962:intron 50.0%
TCTTCCTCGAGTTCCTCCTT+TGG + chr8.2:41493723-41493742 None:intergenic 50.0%
TGGCCCTCCAATCAATTGGA+AGG - chr8.2:41493674-41493693 MS.gene35962:CDS 50.0%
TGTGGAAAACCCTTCACACC+TGG + chr8.2:41494752-41494771 None:intergenic 50.0%
TTAACCAAACAGGGCCAAGG+AGG + chr8.2:41492976-41492995 None:intergenic 50.0%
! AGGGGTGTACCTGCTGTTAT+TGG - chr8.2:41493397-41493416 MS.gene35962:intron 50.0%
! CCGACTCCTAAAGTTTCTGG+CGG - chr8.2:41494301-41494320 MS.gene35962:CDS 50.0%
! CCGCCAGAAACTTTAGGAGT+CGG + chr8.2:41494304-41494323 None:intergenic 50.0%
! CTCGGTGTGATATATGAGCG+AGG - chr8.2:41494598-41494617 MS.gene35962:CDS 50.0%
! TCGAAGGCTCTTGATGGGTA+TGG + chr8.2:41493445-41493464 None:intergenic 50.0%
! TTGGGAGCATGGCAAAGAAG+TGG - chr8.2:41492366-41492385 MS.gene35962:CDS 50.0%
!! GAAAGCACCCAATAACCACC+AGG + chr8.2:41493654-41493673 None:intergenic 50.0%
!! GATAGAGTTCTAAGGCCTGG+TGG - chr8.2:41493636-41493655 MS.gene35962:CDS 50.0%
!! GTTCAAGTTTCTTCTGGGGC+TGG + chr8.2:41495078-41495097 None:intergenic 50.0%
ACCATGGCAGAGACCAAAGG+AGG - chr8.2:41493707-41493726 MS.gene35962:CDS 55.0%
CCAGACATCATCAGGGTCTG+AGG + chr8.2:41493889-41493908 None:intergenic 55.0%
CCCATCAAGAGCCTTCGACA+TGG - chr8.2:41493447-41493466 MS.gene35962:intron 55.0%
CGGATTATGACAGCGCCTTC+TGG + chr8.2:41495635-41495654 None:intergenic 55.0%
GAGACCAAAGGAGGAACTCG+AGG - chr8.2:41493716-41493735 MS.gene35962:CDS 55.0%
GCACGCCGTTGATCTTGACA+CGG + chr8.2:41492567-41492586 None:intergenic 55.0%
GCCATGTCGAAGGCTCTTGA+TGG + chr8.2:41493451-41493470 None:intergenic 55.0%
GTGAGGACAGCACTTGACAC+CGG - chr8.2:41492894-41492913 MS.gene35962:intron 55.0%
TCCTCCTTTGGTCTCTGCCA+TGG + chr8.2:41493711-41493730 None:intergenic 55.0%
TGTGCTTCGTGAGAGTCCCT+CGG + chr8.2:41493358-41493377 None:intergenic 55.0%
! CCTCAGACCCTGATGATGTC+TGG - chr8.2:41493886-41493905 MS.gene35962:intron 55.0%
! TGCCATGTCGTTTGCACCGA+GGG - chr8.2:41493339-41493358 MS.gene35962:intron 55.0%
! TTGCCATGTCGTTTGCACCG+AGG - chr8.2:41493338-41493357 MS.gene35962:intron 55.0%
! ACAGCACTTGACACCGGCTG+TGG - chr8.2:41492900-41492919 MS.gene35962:intron 60.0%
! AGCACTTGACACCGGCTGTG+GGG - chr8.2:41492902-41492921 MS.gene35962:intron 60.0%
! CAGCACTTGACACCGGCTGT+GGG - chr8.2:41492901-41492920 MS.gene35962:intron 60.0%
! GTCCCTCGGTGCAAACGACA+TGG + chr8.2:41493344-41493363 None:intergenic 60.0%
!! AGAGAGGCACTGTCCTCCAG+AGG - chr8.2:41492620-41492639 MS.gene35962:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 41492279 41495817 41492279 ID=MS.gene35962
chr8.2 mRNA 41492279 41495817 41492279 ID=MS.gene35962.t1;Parent=MS.gene35962
chr8.2 exon 41495173 41495817 41495173 ID=MS.gene35962.t1.exon1;Parent=MS.gene35962.t1
chr8.2 CDS 41495173 41495817 41495173 ID=cds.MS.gene35962.t1;Parent=MS.gene35962.t1
chr8.2 exon 41494588 41494798 41494588 ID=MS.gene35962.t1.exon2;Parent=MS.gene35962.t1
chr8.2 CDS 41494588 41494798 41494588 ID=cds.MS.gene35962.t1;Parent=MS.gene35962.t1
chr8.2 exon 41494189 41494483 41494189 ID=MS.gene35962.t1.exon3;Parent=MS.gene35962.t1
chr8.2 CDS 41494189 41494483 41494189 ID=cds.MS.gene35962.t1;Parent=MS.gene35962.t1
chr8.2 exon 41493452 41493823 41493452 ID=MS.gene35962.t1.exon4;Parent=MS.gene35962.t1
chr8.2 CDS 41493452 41493823 41493452 ID=cds.MS.gene35962.t1;Parent=MS.gene35962.t1
chr8.2 exon 41493266 41493343 41493266 ID=MS.gene35962.t1.exon5;Parent=MS.gene35962.t1
chr8.2 CDS 41493266 41493343 41493266 ID=cds.MS.gene35962.t1;Parent=MS.gene35962.t1
chr8.2 exon 41492279 41492510 41492279 ID=MS.gene35962.t1.exon6;Parent=MS.gene35962.t1
chr8.2 CDS 41492279 41492510 41492279 ID=cds.MS.gene35962.t1;Parent=MS.gene35962.t1
Gene Sequence

>MS.gene35962

ATGGCAAAACCAAGTGCTGCTGATAATAGGACTAGAAGTTCAGTGCAGATCTTTATAGTAGTTGGTTTGTGCTGTTTCTTCTATATATTGGGAGCATGGCAAAGAAGTGGTTTTGGAAAAGGAGATAGTATAGCATTGGAGATTACAAAGAATAACGCTGAATGTGATGTAGTTCCAAATTTAAGTTTTGATTCACACCATGCTGGTGAAGTTAGTCAAATCGATGAATCCAATTCGAACACTAAGGTGTTTAAACCTTGTGATGCTCGTTATACCGATTACACTCCGTGTCAAGATCAACGGCGTGCTATGACGTTTCCGAGAGAAAACATGAACTATAGAGAGAGGCACTGTCCTCCAGAGGAAGAGAAGTTACATTGTATGATCCCAGCACCAAAAGGGTATGTAACACCTTTTCCATGGCCTAAGAGTAGGGATTATGTCCCTTATGCTAATGCACCTTACAAGAGTCTCACAGTTGAGAAGGCTATTCAGAATTGGATCCAATATGAGGGAAATGTTTTTAGATTCCCTGGTGGTGGAACACAATTTCCTCAAGGTGCTGATAAATATATTGATCAACTCGCATCTGTTATTCCTATCAATGATGGGACAGTGAGGACAGCACTTGACACCGGCTGTGGGGTATGTTTATGTTTTTATTTATCTCAAATTCCTGATCAGTTTTGTTAAACCTCCTTGGCCCTGTTTGGTTAAACAGCTTAATTGATTACTTATAGTATAAGCTATTTCTGTCACGAAGATAAAATATTGCCAAAGTGTTTTTCCATATAAGCTATATGTTTTTATAAGCTATGGTTGAGAACTTAAAAGAAATAAGCGGAAAACAACTTATCGATATGTCATAAACTGTTTCCATAAGCTCTCCCAAACAGTCTCACAAGTGCGTATGCCAGTAGATAAACTCAAATTAGCCACAATCCAAACTGGCTTGTACATATTACTTTGACTATTCGGTTCATTATTTGTATTAAGCAATGTATGTATTCATATGTCAGGTTGCAAGTTGGGGTGCATATCTTTGGAGCAGAAATGTGGTTGCCATGTCGTTTGCACCGAGGGACTCTCACGAAGCACAAGTACAATTTGCTCTAGAAAGGGGTGTACCTGCTGTTATTGGTGTTTTTGGAACCATAAAATTGCCATACCCATCAAGAGCCTTCGACATGGCTCATTGTTCTCGCTGTTTGATTCCATGGGGAGCAAATGGTAAGAACATCAAAGTTAAAGTAAACAATGTTTTACCATGTAAAACTATTAGCTGCAAGTAAAGAAGCACGATTTGACATTATATTTTGGTTGTTGATTTGCAGATGGAATGTATATGATGGAAGTTGATAGAGTTCTAAGGCCTGGTGGTTATTGGGTGCTTTCTGGCCCTCCAATCAATTGGAAGGTTAACTACAAACCATGGCAGAGACCAAAGGAGGAACTCGAGGAAGAACAAAGAAAGATTGAAGAGGTTGCTAAGAAACTTTGCTGGGAGAAGAAGTCTGAGAAGGCTGAAATTGCAATTTGGCAAAAGATGACAGACACCGAGTCATGTCGTAGCAGACAAGACGACTCCAGTGTAGAATTTTGCGAATCCTCAGACCCTGATGATGTCTGGTACGCATTCTTACCCCCCTTTTTGTTGTTATATAATAAATATTTGTTTATTGATGTTCCTTTTAGTTCTCCCTACTTGTCATAGGAACATACATGTGTGTGATATAGTTCTAAATTGCTATTGCGGTTAAACTCAAACCTTTATATTGCAGAAAAATGAGACAATTTTAAAATCATGCATCCGAGCATACGACTAAAAGAATCTGATATTCTCTAGTAATGTTTTAGATATTGACACTTGTTGTACTGTATGTTATTGATGTCGTCTCTTGATTTTTTTTAATTGTATAAATATGAGCAATGTTTAATGTTGTATAGAAATAAGAGGTGTACTGACTGGAAATGCTTTTGTTCTGTGGCTATAGGTATAAGAAAATGAAGGCATGCGTTACTCCGACTCCTAAAGTTTCTGGCGGCGATCTTAAACCATTTCCAAACAGGCTATATGCGATCCCTCCTAGAGTTTCTAGTGGTTCTATTCCTGGAGTTTCTTCTGAGACATACCAGGATGATAACAAAATGTGGAAAAAGCATGTCAATGCATACAAGAAAATTAATTCACTGTTGGATTCCGGAAGATATCGCAACATTATGGATATGAATGCTGGTTTGGGTAGTTTTGCTGCCGCTATTCATTCATCTAAGTCATGGGTCATGAATGTTGTGCCTACTATAGCTGAAAAAAGTACACTCGGTGTGATATATGAGCGAGGACTGATTGGCATCTATCATGATTGGTATGGTTTCCTAACTATTCGATCCTATTTTATTCATGTCAGATCTCGATCTTTAACTATTCGATCCTGTTCTTTTTTCTTATATATTTTTGTTGGTTTTTTATCCAGGTGTGAAGGGTTTTCCACATATCCAAGAACATACGATCTCATTCATGCTAATGGCCTCTTTAGTTTGTACCAGGATAAGTAAGTGTTCCTTCGGTCAAATGACTATTTAACTATTCGTCTTGTTAAGTTTTATAAGCCAGTTCTTATTCTGTAGGCGTAGACAACCAACTTCACACAGTATTGCATTGGGATCTGTAGTGACCTTGTTTGTTGAGTCTACAAACTGTTACATGGTTAAAAAAACACTTTATACATGTGCTTGAGTGTGTCCATTTCAGTCCCTAAATATGGTCATTATAAAAAATATGAAAAAATTACACTCCCAGCCCCAGAAGAAACTTGAACTACTCTCCCCTCCCATCTTACGTTAAAACCTACACTACTCTCGATGATAAGTGAATATGCACATTTTAATAAAAATTAGGATTCTTATGCACATTTTTTTCTTCAATTTTGGCTCTTATTATCTTTTTTTTTTTTTTTACATATAGTTTTGAAATCTTACTAGTATCAATGTTAATTTATACCAACATAAAACAATAAAGATAAACTACAGTTCTGAGCATTCTTTCGAAGCAGATAAAATGGATATTAAAATCTTGATTTCATCTTCTAAAGTCATACAATCAATATAAGGTGTTCAAAATATTGAAAATCAATAATAACAGTTTTTGCATTTGTTACTCTTTTTTTGTTTTGTTTAATTCAGCATTCAACCGTGTCTTGATTTTGCATACCATTAGCAGAAAACTTGTTTTTAGTTTCGTGAGTTCTCTTTTATAGCTGTGTTCTTTCATGTGACTCAAGCATGTCTATGATGTAATCAAATGCAGATGCAATACAGAAGACATTCTTCTCGAGATGGACCGAATATTGCGACCAGAAGGCGCTGTCATAATCCGCGATGAAGTCGATGTGTTAATTAAGGTAAAGAAGTTAATCGGAGGAATGAGATGGAATATGAAATTGGTCGATCATGAAGATGGTCCTCTTGTTCCTGAGAAAGTACTAATTGCTGTCAAACAGTATTGGGTTACTGATGGAAACTCCACATCAACACAATAA
Protein sequence

>MS.gene35962.t1

MAKPSAADNRTRSSVQIFIVVGLCCFFYILGAWQRSGFGKGDSIALEITKNNAECDVVPNLSFDSHHAGEVSQIDESNSNTKVFKPCDARYTDYTPCQDQRRAMTFPRENMNYRERHCPPEEEKLHCMIPAPKGYVTPFPWPKSRDYVPYANAPYKSLTVEKAIQNWIQYEGNVFRFPGGGTQFPQGADKYIDQLASVIPINDGTVRTALDTGCGVASWGAYLWSRNVVAMSFAPRDSHEAQVQFALERGVPAVIGVFGTIKLPYPSRAFDMAHCSRCLIPWGANDGMYMMEVDRVLRPGGYWVLSGPPINWKVNYKPWQRPKEELEEEQRKIEEVAKKLCWEKKSEKAEIAIWQKMTDTESCRSRQDDSSVEFCESSDPDDVWYKKMKACVTPTPKVSGGDLKPFPNRLYAIPPRVSSGSIPGVSSETYQDDNKMWKKHVNAYKKINSLLDSGRYRNIMDMNAGLGSFAAAIHSSKSWVMNVVPTIAEKSTLGVIYERGLIGIYHDWCEGFSTYPRTYDLIHANGLFSLYQDKCNTEDILLEMDRILRPEGAVIIRDEVDVLIKVKKLIGGMRWNMKLVDHEDGPLVPEKVLIAVKQYWVTDGNSTSTQ