Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36144.t1 | XP_013457200.1 | 99.5 | 384 | 2 | 0 | 1 | 384 | 1 | 384 | 1.60E-223 | 785 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36144.t1 | Q66KH2 | 49.0 | 388 | 188 | 4 | 3 | 382 | 2 | 387 | 2.8e-104 | 380.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36144.t1 | A0A072UPC6 | 99.5 | 384 | 2 | 0 | 1 | 384 | 1 | 384 | 1.1e-223 | 785.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene36144 | 0.813368 | 2.65E-51 | -1.69E-46 |
| MS.gene050945 | MS.gene36144 | 0.812956 | 3.27E-51 | -1.69E-46 |
| MS.gene051959 | MS.gene36144 | 0.817395 | 3.37E-52 | -1.69E-46 |
| MS.gene051960 | MS.gene36144 | 0.821153 | 4.70E-53 | -1.69E-46 |
| MS.gene054413 | MS.gene36144 | 0.825014 | 5.91E-54 | -1.69E-46 |
| MS.gene056530 | MS.gene36144 | 0.837628 | 4.65E-57 | -1.69E-46 |
| MS.gene057001 | MS.gene36144 | 0.80562 | 1.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36144.t1 | MTR_4g094255 | 99.479 | 384 | 2 | 0 | 1 | 384 | 1 | 384 | 0.0 | 795 |
| MS.gene36144.t1 | MTR_4g094255 | 99.322 | 295 | 2 | 0 | 90 | 384 | 1 | 295 | 0.0 | 614 |
| MS.gene36144.t1 | MTR_5g062720 | 68.052 | 385 | 121 | 1 | 1 | 383 | 1 | 385 | 0.0 | 591 |
| MS.gene36144.t1 | MTR_5g062720 | 67.987 | 303 | 97 | 0 | 1 | 303 | 1 | 303 | 9.89e-166 | 466 |
| MS.gene36144.t1 | MTR_3g081480 | 35.890 | 365 | 201 | 8 | 8 | 368 | 7 | 342 | 9.06e-73 | 231 |
| MS.gene36144.t1 | MTR_2g083260 | 25.503 | 298 | 185 | 10 | 104 | 382 | 199 | 478 | 4.10e-18 | 86.3 |
| MS.gene36144.t1 | MTR_2g083260 | 33.333 | 108 | 71 | 1 | 6 | 112 | 5 | 112 | 4.80e-13 | 70.9 |
| MS.gene36144.t1 | MTR_4g080800 | 33.166 | 199 | 102 | 8 | 202 | 382 | 290 | 475 | 8.01e-17 | 82.0 |
| MS.gene36144.t1 | MTR_4g080800 | 33.645 | 107 | 70 | 1 | 6 | 111 | 5 | 111 | 2.54e-13 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36144.t1 | AT1G36050 | 70.390 | 385 | 112 | 1 | 1 | 383 | 1 | 385 | 0.0 | 592 |
| MS.gene36144.t1 | AT1G36050 | 70.419 | 382 | 111 | 1 | 1 | 380 | 1 | 382 | 0.0 | 587 |
| MS.gene36144.t1 | AT1G22200 | 69.091 | 385 | 117 | 1 | 1 | 383 | 1 | 385 | 0.0 | 580 |
| MS.gene36144.t1 | AT1G22200 | 68.657 | 335 | 103 | 1 | 1 | 333 | 1 | 335 | 7.63e-179 | 500 |
| MS.gene36144.t1 | AT3G22290 | 36.483 | 381 | 201 | 10 | 4 | 377 | 3 | 349 | 1.56e-71 | 228 |
| MS.gene36144.t1 | AT3G22290 | 36.483 | 381 | 201 | 10 | 4 | 377 | 3 | 349 | 1.56e-71 | 228 |
| MS.gene36144.t1 | AT4G27080 | 30.631 | 222 | 122 | 9 | 179 | 382 | 271 | 478 | 9.90e-19 | 88.2 |
| MS.gene36144.t1 | AT4G27080 | 31.818 | 110 | 74 | 1 | 6 | 114 | 5 | 114 | 2.36e-13 | 71.6 |
| MS.gene36144.t1 | AT4G27080 | 30.631 | 222 | 122 | 9 | 179 | 382 | 323 | 530 | 1.13e-18 | 88.2 |
| MS.gene36144.t1 | AT4G27080 | 32.039 | 103 | 69 | 1 | 13 | 114 | 64 | 166 | 3.47e-11 | 65.1 |
| MS.gene36144.t1 | AT1G50950 | 37.037 | 108 | 67 | 1 | 6 | 112 | 5 | 112 | 7.22e-15 | 76.3 |
| MS.gene36144.t1 | AT1G50950 | 25.887 | 282 | 166 | 10 | 124 | 382 | 226 | 487 | 1.87e-11 | 65.9 |
| MS.gene36144.t1 | AT1G50950 | 37.037 | 108 | 67 | 1 | 6 | 112 | 5 | 112 | 7.47e-15 | 76.3 |
| MS.gene36144.t1 | AT1G50950 | 25.887 | 282 | 166 | 10 | 124 | 382 | 221 | 482 | 2.04e-11 | 65.9 |
| MS.gene36144.t1 | AT3G20560 | 28.856 | 201 | 111 | 7 | 202 | 382 | 293 | 481 | 2.38e-14 | 74.7 |
| MS.gene36144.t1 | AT3G20560 | 35.185 | 108 | 69 | 1 | 6 | 112 | 5 | 112 | 1.01e-13 | 72.8 |
Find 88 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTACTCTTACCTTTAT+TGG | 0.086581 | 4.2:+18963569 | None:intergenic |
| GACGATTACATTGATGTTTA+AGG | 0.122289 | 4.2:+18968422 | None:intergenic |
| TCTTCAGTCAGCTATCTTTC+TGG | 0.129021 | 4.2:-18965715 | MS.gene36144:CDS |
| TAATGCTTTGCAGGATAAAA+TGG | 0.150023 | 4.2:-18964207 | MS.gene36144:intron |
| GGCTGATCTACTTGCTTTGC+AGG | 0.231010 | 4.2:-18965694 | MS.gene36144:CDS |
| CTTCAACAAGCTCCGCAATT+TGG | 0.259431 | 4.2:-18969509 | MS.gene36144:CDS |
| TACAAACCTTTATGAAATAT+TGG | 0.288463 | 4.2:+18964158 | None:intergenic |
| CAGATCAACAAATTAAGTTT+TGG | 0.299273 | 4.2:-18965513 | MS.gene36144:CDS |
| ACCAATATTTGTGCAATCAT+TGG | 0.300995 | 4.2:-18962725 | MS.gene36144:CDS |
| AAATATTGTGGTTCTTGTTT+TGG | 0.315971 | 4.2:-18967497 | MS.gene36144:CDS |
| GTGGATTCATCAATATATTA+TGG | 0.317761 | 4.2:-18962570 | MS.gene36144:CDS |
| TCCAATGATTGCACAAATAT+TGG | 0.332241 | 4.2:+18962724 | None:intergenic |
| AGAACTGGGTGCTGCAGTTC+CGG | 0.349349 | 4.2:-18963618 | MS.gene36144:CDS |
| GAACTGGGTGCTGCAGTTCC+GGG | 0.369702 | 4.2:-18963617 | MS.gene36144:CDS |
| TGTTACAGAGAGTAAGCTTT+TGG | 0.377097 | 4.2:-18968466 | MS.gene36144:CDS |
| GGGATGGGCACTGACAAATA+TGG | 0.383809 | 4.2:-18966127 | MS.gene36144:CDS |
| CTATGACATATCTCCAATAA+AGG | 0.384793 | 4.2:-18963582 | MS.gene36144:intron |
| GCATTTCAAGAGTTCAGAAC+TGG | 0.386769 | 4.2:-18963633 | MS.gene36144:CDS |
| AAACAAAGAATTGATGCTAA+TGG | 0.405935 | 4.2:-18967943 | MS.gene36144:CDS |
| GGATTGGTTAATCCTCTTGA+TGG | 0.406458 | 4.2:-18965477 | MS.gene36144:CDS |
| ATAGGTATATTCACAATTGC+GGG | 0.416093 | 4.2:-18962597 | MS.gene36144:intron |
| ATCAAGAAGAAGATGGAGAT+TGG | 0.425527 | 4.2:-18962537 | MS.gene36144:CDS |
| CAAAGTGATAGAAGCTAGAA+AGG | 0.432471 | 4.2:-18967920 | MS.gene36144:CDS |
| GGTGAAGGATGCAATATTCA+TGG | 0.439184 | 4.2:-18965795 | MS.gene36144:CDS |
| AACTTAATTTGTTGATCTGA+TGG | 0.440792 | 4.2:+18965517 | None:intergenic |
| TCGTGTTATATATTCAAATC+AGG | 0.444045 | 4.2:-18963754 | MS.gene36144:intron |
| AAATATGGATTTGATCGATC+AGG | 0.446849 | 4.2:-18966112 | MS.gene36144:intron |
| AAGAAGTTCGTGAGGCATAT+AGG | 0.446884 | 4.2:-18966155 | MS.gene36144:CDS |
| TCAAGAGGATTAACCAATCC+AGG | 0.448600 | 4.2:+18965480 | None:intergenic |
| CATGTACCAATATTTCATAA+AGG | 0.449326 | 4.2:-18964164 | MS.gene36144:intron |
| CCTGCCACCATGTTTCTGTA+AGG | 0.455242 | 4.2:+18967535 | None:intergenic |
| TTAGACACAATGGATATTAG+TGG | 0.459541 | 4.2:-18968106 | MS.gene36144:CDS |
| ACATGCCATGATCATTTCCT+TGG | 0.466746 | 4.2:+18964182 | None:intergenic |
| GAAGCTAGAAAGGAAGGAAT+TGG | 0.469298 | 4.2:-18967910 | MS.gene36144:CDS |
| ACAGTATACACGGACATTAG+AGG | 0.477413 | 4.2:-18963777 | MS.gene36144:CDS |
| TGGTCACCATTACCCTGGAT+TGG | 0.479452 | 4.2:-18965493 | MS.gene36144:CDS |
| GTGATAGAAGCTAGAAAGGA+AGG | 0.482180 | 4.2:-18967916 | MS.gene36144:CDS |
| AGATTGGAAAATATAGATGA+TGG | 0.489952 | 4.2:-18962521 | None:intergenic |
| ATTCACAATTGCGGGAATTG+TGG | 0.490506 | 4.2:-18962589 | MS.gene36144:CDS |
| GAAAACAATCAAGAAGAAGA+TGG | 0.504536 | 4.2:-18962544 | MS.gene36144:CDS |
| CTTGTTGAAGACTTTATCCA+TGG | 0.505504 | 4.2:+18969522 | None:intergenic |
| TTTCCCTATTGCAGATTGAA+AGG | 0.505700 | 4.2:-18967559 | MS.gene36144:intron |
| AATATTTGTGCAATCATTGG+AGG | 0.520187 | 4.2:-18962722 | MS.gene36144:intron |
| CTAATGTCCGTGTATACTGT+AGG | 0.520758 | 4.2:+18963780 | None:intergenic |
| GTTGCAGGTCAACTTTAAAG+AGG | 0.521061 | 4.2:-18962774 | MS.gene36144:intron |
| GATTGGTTAATCCTCTTGAT+GGG | 0.521240 | 4.2:-18965476 | MS.gene36144:intron |
| TTCTGAACTCTTGAAATGCT+CGG | 0.529904 | 4.2:+18963637 | None:intergenic |
| CTTGAAGTGAATAAAGTGGC+AGG | 0.531002 | 4.2:-18965768 | MS.gene36144:CDS |
| AATGCTTTGCAGGATAAAAT+GGG | 0.531280 | 4.2:-18964206 | MS.gene36144:intron |
| TCGTGAGGCATATAGGAAGA+AGG | 0.532464 | 4.2:-18966148 | MS.gene36144:CDS |
| TAGCTGACTGAAGAAAGCTT+TGG | 0.540463 | 4.2:+18965724 | None:intergenic |
| GGGTCATATCATACTTAGCT+CGG | 0.541362 | 4.2:+18969387 | None:intergenic |
| TACTTAGCTCGGAAATAAAG+AGG | 0.546697 | 4.2:+18969398 | None:intergenic |
| GATTAACCAATCCAGGGTAA+TGG | 0.548392 | 4.2:+18965487 | None:intergenic |
| AGGCATATAGGAAGAAGGGA+TGG | 0.551318 | 4.2:-18966143 | MS.gene36144:CDS |
| CCTTACAGAAACATGGTGGC+AGG | 0.551937 | 4.2:-18967535 | MS.gene36144:CDS |
| GAGGAAAAGCATGACGGCGG+CGG | 0.554845 | 4.2:+18969417 | None:intergenic |
| CATAGGTATATTCACAATTG+CGG | 0.556363 | 4.2:-18962598 | MS.gene36144:intron |
| TGGGTCCAAGGAAATGATCA+TGG | 0.561072 | 4.2:-18964187 | MS.gene36144:CDS |
| TATAACCGCACTCTCGCCGG+AGG | 0.565571 | 4.2:-18969457 | MS.gene36144:CDS |
| CTGCCACCATGTTTCTGTAA+GGG | 0.566564 | 4.2:+18967536 | None:intergenic |
| AAAGAAAATTACCCATCAAG+AGG | 0.569599 | 4.2:+18965465 | None:intergenic |
| TAATGTCCGTGTATACTGTA+GGG | 0.574539 | 4.2:+18963781 | None:intergenic |
| AGTATTGAACATCGAACAGC+AGG | 0.576281 | 4.2:+18968133 | None:intergenic |
| GGAAGGAATTGGCGCACCAA+AGG | 0.576811 | 4.2:-18967899 | MS.gene36144:intron |
| ACTCTTGAAATGCTCGGTCA+CGG | 0.579209 | 4.2:+18963643 | None:intergenic |
| GACGACGGTGACGACACCTC+CGG | 0.579378 | 4.2:+18969441 | None:intergenic |
| TTGGGTATGCATCCAAATTG+CGG | 0.580291 | 4.2:+18969497 | None:intergenic |
| CGTGAGGCATATAGGAAGAA+GGG | 0.582433 | 4.2:-18966147 | MS.gene36144:CDS |
| TCATAGAAGAAGAAAACTCC+CGG | 0.587428 | 4.2:+18963599 | None:intergenic |
| CATGACGGCGGCGGAGACGA+CGG | 0.590043 | 4.2:+18969426 | None:intergenic |
| ATCTCTTGAAGTGAATAAAG+TGG | 0.597114 | 4.2:-18965772 | MS.gene36144:CDS |
| TGCAGGATAAAATGGGTCCA+AGG | 0.598124 | 4.2:-18964199 | MS.gene36144:intron |
| CATTTCAAGAGTTCAGAACT+GGG | 0.599994 | 4.2:-18963632 | MS.gene36144:CDS |
| AATACTCAGTTTAGACACAA+TGG | 0.600279 | 4.2:-18968116 | MS.gene36144:CDS |
| CTTCACAATTATTACAACAG+TGG | 0.607704 | 4.2:+18966175 | None:intergenic |
| CAAAAGGTAAAGGATGAAGA+AGG | 0.608037 | 4.2:-18965816 | MS.gene36144:CDS |
| GTAAAGGATGAAGAAGGTGA+AGG | 0.610725 | 4.2:-18965810 | MS.gene36144:CDS |
| CAAGAGGATTAACCAATCCA+GGG | 0.627731 | 4.2:+18965481 | None:intergenic |
| CGACACCTCCGGCGAGAGTG+CGG | 0.635707 | 4.2:+18969452 | None:intergenic |
| GGCATATAGGAAGAAGGGAT+GGG | 0.636215 | 4.2:-18966142 | MS.gene36144:CDS |
| GAAAGGCCCTTACAGAAACA+TGG | 0.652386 | 4.2:-18967542 | MS.gene36144:CDS |
| GGTGGTCCCTACAGTATACA+CGG | 0.683793 | 4.2:-18963787 | MS.gene36144:CDS |
| AATAAAGAGGAAAAGCATGA+CGG | 0.687611 | 4.2:+18969411 | None:intergenic |
| TAATTGTGAAGAAGTTCGTG+AGG | 0.690238 | 4.2:-18966163 | MS.gene36144:CDS |
| CAATGGATATTAGTGGCGAG+CGG | 0.694408 | 4.2:-18968099 | MS.gene36144:CDS |
| AAAGAGGAAAAGCATGACGG+CGG | 0.739902 | 4.2:+18969414 | None:intergenic |
| AGGCCCTTACAGAAACATGG+TGG | 0.756459 | 4.2:-18967539 | MS.gene36144:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 18962524 | 18969542 | 18962524 | ID=MS.gene36144 |
| chr4.2 | mRNA | 18962524 | 18969542 | 18962524 | ID=MS.gene36144.t1;Parent=MS.gene36144 |
| chr4.2 | exon | 18969401 | 18969542 | 18969401 | ID=MS.gene36144.t1.exon1;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18969401 | 18969542 | 18969401 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18968431 | 18968504 | 18968431 | ID=MS.gene36144.t1.exon2;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18968431 | 18968504 | 18968431 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18968087 | 18968170 | 18968087 | ID=MS.gene36144.t1.exon3;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18968087 | 18968170 | 18968087 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18967900 | 18967980 | 18967900 | ID=MS.gene36144.t1.exon4;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18967900 | 18967980 | 18967900 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18967487 | 18967567 | 18967487 | ID=MS.gene36144.t1.exon5;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18967487 | 18967567 | 18967487 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18966113 | 18966205 | 18966113 | ID=MS.gene36144.t1.exon6;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18966113 | 18966205 | 18966113 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18965680 | 18965859 | 18965680 | ID=MS.gene36144.t1.exon7;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18965680 | 18965859 | 18965680 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18965477 | 18965544 | 18965477 | ID=MS.gene36144.t1.exon8;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18965477 | 18965544 | 18965477 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18964165 | 18964216 | 18964165 | ID=MS.gene36144.t1.exon9;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18964165 | 18964216 | 18964165 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18963755 | 18963808 | 18963755 | ID=MS.gene36144.t1.exon10;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18963755 | 18963808 | 18963755 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18963583 | 18963669 | 18963583 | ID=MS.gene36144.t1.exon11;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18963583 | 18963669 | 18963583 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18962723 | 18962789 | 18962723 | ID=MS.gene36144.t1.exon12;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18962723 | 18962789 | 18962723 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| chr4.2 | exon | 18962524 | 18962615 | 18962524 | ID=MS.gene36144.t1.exon13;Parent=MS.gene36144.t1 |
| chr4.2 | CDS | 18962524 | 18962615 | 18962524 | ID=cds.MS.gene36144.t1;Parent=MS.gene36144.t1 |
| Gene Sequence |
| Protein sequence |
