AlfalfaGEDB | MS.gene36543

M. sativa cultivar XinJiangDaYe / MS.gene36543

KEGG: K02907
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0015934 GO:0019538 GO:0032991 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044391 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1990904
PFAM: PF00327.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36543.t1	XP_013444321.1	100	106	0	0	1	106	1	106	2.00E-51	211.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36543.t1	I3S856	100.0	106	0	0	1	106	1	106	1.4e-51	211.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050621	MS.gene36543	0.800925	1.15E-48	-1.69E-46
MS.gene058223	MS.gene36543	0.802489	5.47E-49	-1.69E-46
MS.gene058429	MS.gene36543	-0.80773	4.38E-50	-1.69E-46
MS.gene060122	MS.gene36543	0.818852	1.58E-52	-1.69E-46
MS.gene060125	MS.gene36543	0.800047	1.73E-48	-1.69E-46
MS.gene06052	MS.gene36543	-0.812011	5.25E-51	-1.69E-46
MS.gene060932	MS.gene36543	-0.826874	2.14E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36543.t1	MTR_8g016300	100.000	106	0	0	1	106	1	106	2.47e-76	220
MS.gene36543.t1	MTR_8g099150	98.113	106	2	0	1	106	1	106	4.01e-75	217

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene36543.t1	AT5G55140	64.762	105	36	1	3	106	1	105	6.44e-46	144

Find 42 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTCTAGTGAGGGGTATTC+CGG	0.253922	8.2:-76836133	MS.gene36543:CDS
CACTAGAGTTATATAAAGAT+TGG	0.372018	8.2:+76836146	None:intergenic
TGTATTTGATATAGGTTAAG+AGG	0.379656	8.2:-76835930	MS.gene36543:intron
TCAACCTGCTTCTTCTGCTT+AGG	0.384301	8.2:-76835815	None:intergenic
CAACCTGCTTCTTCTGCTTA+GGG	0.390606	8.2:-76835814	None:intergenic
CATGAGCATGAATGCGTTCA+AGG	0.398457	8.2:-76836202	MS.gene36543:CDS
GATGGAACACACCTACTGTT+AGG	0.400025	8.2:-76836041	MS.gene36543:CDS
ACTCTAGTGAGGGGTATTCC+GGG	0.407993	8.2:-76836132	MS.gene36543:CDS
AGATTGGGGCTATAAGCAAT+TGG	0.415456	8.2:+76836162	None:intergenic
ACTAGAGTTATATAAAGATT+GGG	0.420712	8.2:+76836147	None:intergenic
AAGCAGAAGAAGCAGGTTGA+TGG	0.435151	8.2:+76835818	None:intergenic
AGTGCGCCTATGTTGCTTCC+TGG	0.448328	8.2:+76836107	None:intergenic
CTATGTTGCTTCCTGGTCCC+CGG	0.462046	8.2:+76836114	None:intergenic
ATGGAACACACCTACTGTTA+GGG	0.464145	8.2:-76836040	MS.gene36543:CDS
GTACAAGGCCCGCAAAGAAA+AGG	0.493013	8.2:-76835881	MS.gene36543:CDS
GATTGGGGCTATAAGCAATT+GGG	0.504706	8.2:+76836163	None:intergenic
CGGGGACCAGGAAGCAACAT+AGG	0.508472	8.2:-76836113	MS.gene36543:CDS
TGTTCCATCGCATGACAGTT+CGG	0.511917	8.2:+76836055	None:intergenic
GAAGCAACATAGGCGCACTT+TGG	0.520220	8.2:-76836103	MS.gene36543:CDS
TGAGGGGTATTCCGGGGACC+AGG	0.527749	8.2:-76836125	MS.gene36543:CDS
CCGAGCTCTTCGTCCTCCGT+TGG	0.538228	8.2:-76835848	MS.gene36543:CDS
CGGAGGACGAAGAGCTCGGT+GGG	0.539550	8.2:+76835852	None:intergenic
CTTGAACGCATTCATGCTCA+TGG	0.548540	8.2:+76836203	None:intergenic
GTACCCTAAGCAGAAGAAGC+AGG	0.555500	8.2:+76835811	None:intergenic
GCTAGTTGTTGTTGAGACAG+AGG	0.564385	8.2:-76835908	MS.gene36543:CDS
TGTTAGGGGAATGATCCAGC+AGG	0.585091	8.2:-76836025	MS.gene36543:intron
GCTATAAGCAATTGGGACAC+AGG	0.590348	8.2:+76836170	None:intergenic
GGTTGATGGTTTATGACCAA+CGG	0.595496	8.2:+76835832	None:intergenic
TCTTTATATAACTCTAGTGA+GGG	0.599888	8.2:-76836142	MS.gene36543:CDS
GCAACCGAACTGTCATGCGA+TGG	0.602415	8.2:-76836059	MS.gene36543:CDS
CTAGAGTTATATAAAGATTG+GGG	0.610839	8.2:+76836148	None:intergenic
ATCTTTATATAACTCTAGTG+AGG	0.616129	8.2:-76836143	MS.gene36543:CDS
TGGATCATTCCCCTAACAGT+AGG	0.620579	8.2:+76836030	None:intergenic
CAAGGCCCGCAAAGAAAAGG+AGG	0.626844	8.2:-76835878	MS.gene36543:CDS
CCAACGGAGGACGAAGAGCT+CGG	0.633862	8.2:+76835848	None:intergenic
TGAGACAGAGGAGATGTACA+AGG	0.642473	8.2:-76835896	MS.gene36543:CDS
TGGAACACACCTACTGTTAG+GGG	0.654422	8.2:-76836039	MS.gene36543:CDS
CTCTAGTGAGGGGTATTCCG+GGG	0.659985	8.2:-76836131	MS.gene36543:CDS
ACTTTGGAAGCACTACGTCT+CGG	0.676793	8.2:-76836087	MS.gene36543:CDS
ACGGAGGACGAAGAGCTCGG+TGG	0.687356	8.2:+76835851	None:intergenic
CTTTATATAACTCTAGTGAG+GGG	0.705279	8.2:-76836141	MS.gene36543:CDS
TGATGGTTTATGACCAACGG+AGG	0.718299	8.2:+76835835	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACTAGAGTTATATAAAGATT+GGG	+	chr8.2:76835873-76835892	None:intergenic	20.0%
!	ATCTTTATATAACTCTAGTG+AGG	-	chr8.2:76835874-76835893	MS.gene36543:CDS	25.0%
!	CACTAGAGTTATATAAAGAT+TGG	+	chr8.2:76835874-76835893	None:intergenic	25.0%
!	CTAGAGTTATATAAAGATTG+GGG	+	chr8.2:76835872-76835891	None:intergenic	25.0%
!	GCAAACTTTGTATTTGATAT+AGG	-	chr8.2:76836079-76836098	MS.gene36543:CDS	25.0%
!	TCTTTATATAACTCTAGTGA+GGG	-	chr8.2:76835875-76835894	MS.gene36543:CDS	25.0%
!	TGTATTTGATATAGGTTAAG+AGG	-	chr8.2:76836087-76836106	MS.gene36543:CDS	25.0%
	CTTTATATAACTCTAGTGAG+GGG	-	chr8.2:76835876-76835895	MS.gene36543:CDS	30.0%
!	GCAGATATGTCATTTCAAAA+TGG	+	chr8.2:76836040-76836059	None:intergenic	30.0%
	TGCCTGTATACTAACAACAT+CGG	-	chr8.2:76836057-76836076	MS.gene36543:CDS	35.0%
	AGATTGGGGCTATAAGCAAT+TGG	+	chr8.2:76835858-76835877	None:intergenic	40.0%
	ATGGAACACACCTACTGTTA+GGG	-	chr8.2:76835977-76835996	MS.gene36543:intron	40.0%
	GATTGGGGCTATAAGCAATT+GGG	+	chr8.2:76835857-76835876	None:intergenic	40.0%
	TGCCGATGTTGTTAGTATAC+AGG	+	chr8.2:76836062-76836081	None:intergenic	40.0%
!!	GGTTGATGGTTTATGACCAA+CGG	+	chr8.2:76836188-76836207	None:intergenic	40.0%
	AGAGCTATGTTGAAACCTGC+TGG	+	chr8.2:76836010-76836029	None:intergenic	45.0%
	GATGGAACACACCTACTGTT+AGG	-	chr8.2:76835976-76835995	MS.gene36543:intron	45.0%
	GCTATAAGCAATTGGGACAC+AGG	+	chr8.2:76835850-76835869	None:intergenic	45.0%
	TGAGACAGAGGAGATGTACA+AGG	-	chr8.2:76836121-76836140	MS.gene36543:CDS	45.0%
	TGATGGTTTATGACCAACGG+AGG	+	chr8.2:76836185-76836204	None:intergenic	45.0%
	TGGAACACACCTACTGTTAG+GGG	-	chr8.2:76835978-76835997	MS.gene36543:intron	45.0%
	TGGATCATTCCCCTAACAGT+AGG	+	chr8.2:76835990-76836009	None:intergenic	45.0%
	TGTTCCATCGCATGACAGTT+CGG	+	chr8.2:76835965-76835984	None:intergenic	45.0%
!	AAGCAGAAGAAGCAGGTTGA+TGG	+	chr8.2:76836202-76836221	None:intergenic	45.0%
!	GCTAGTTGTTGTTGAGACAG+AGG	-	chr8.2:76836109-76836128	MS.gene36543:CDS	45.0%
!!	AACTCTAGTGAGGGGTATTC+CGG	-	chr8.2:76835884-76835903	MS.gene36543:CDS	45.0%
!!	ACTTTGGAAGCACTACGTCT+CGG	-	chr8.2:76835930-76835949	MS.gene36543:intron	45.0%
	GAAGCAACATAGGCGCACTT+TGG	-	chr8.2:76835914-76835933	MS.gene36543:CDS	50.0%
	GTACAAGGCCCGCAAAGAAA+AGG	-	chr8.2:76836136-76836155	MS.gene36543:CDS	50.0%
	TGTTAGGGGAATGATCCAGC+AGG	-	chr8.2:76835992-76836011	MS.gene36543:intron	50.0%
!	GGCTTCCTCCTTTTCTTTGC+GGG	+	chr8.2:76836147-76836166	None:intergenic	50.0%
!	GGGCTTCCTCCTTTTCTTTG+CGG	+	chr8.2:76836148-76836167	None:intergenic	50.0%
!!	ACTCTAGTGAGGGGTATTCC+GGG	-	chr8.2:76835885-76835904	MS.gene36543:CDS	50.0%
	CAAGGCCCGCAAAGAAAAGG+AGG	-	chr8.2:76836139-76836158	MS.gene36543:CDS	55.0%
	GCAACCGAACTGTCATGCGA+TGG	-	chr8.2:76835958-76835977	MS.gene36543:intron	55.0%
!	AGTGCGCCTATGTTGCTTCC+TGG	+	chr8.2:76835913-76835932	None:intergenic	55.0%
!	CTATGTTGCTTCCTGGTCCC+CGG	+	chr8.2:76835906-76835925	None:intergenic	55.0%
!!	CTCTAGTGAGGGGTATTCCG+GGG	-	chr8.2:76835886-76835905	MS.gene36543:CDS	55.0%
	CCAACGGAGGACGAAGAGCT+CGG	+	chr8.2:76836172-76836191	None:intergenic	60.0%
	CGGGGACCAGGAAGCAACAT+AGG	-	chr8.2:76835904-76835923	MS.gene36543:CDS	60.0%
	ACGGAGGACGAAGAGCTCGG+TGG	+	chr8.2:76836169-76836188	None:intergenic	65.0%
	CCGAGCTCTTCGTCCTCCGT+TGG	-	chr8.2:76836169-76836188	MS.gene36543:CDS	65.0%
	CGGAGGACGAAGAGCTCGGT+GGG	+	chr8.2:76836168-76836187	None:intergenic	65.0%
!!	TGAGGGGTATTCCGGGGACC+AGG	-	chr8.2:76835892-76835911	MS.gene36543:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	76835816	76836223	76835816	ID=MS.gene36543
chr8.2	mRNA	76835816	76836223	76835816	ID=MS.gene36543.t1;Parent=MS.gene36543
chr8.2	exon	76836026	76836223	76836026	ID=MS.gene36543.t1.exon1;Parent=MS.gene36543.t1
chr8.2	CDS	76836026	76836223	76836026	ID=cds.MS.gene36543.t1;Parent=MS.gene36543.t1
chr8.2	exon	76835816	76835938	76835816	ID=MS.gene36543.t1.exon2;Parent=MS.gene36543.t1
chr8.2	CDS	76835816	76835938	76835816	ID=cds.MS.gene36543.t1;Parent=MS.gene36543.t1

Gene Sequence

>MS.gene36543

ATGAGCATGAATGCGTTCAAGGCATTTAAGTCCTGTGTCCCAATTGCTTATAGCCCCAATCTTTATATAACTCTAGTGAGGGGTATTCCGGGGACCAGGAAGCAACATAGGCGCACTTTGGAAGCACTACGTCTCGGCAAATGCAACCGAACTGTCATGCGATGGAACACACCTACTGTTAGGGGAATGATCCAGCAGGTTTCAACATAGCTCTATCAAATCCATTTTGAAATGACATATCTGCCTGTATACTAACAACATCGGCAAACTTTGTATTTGATATAGGTTAAGAGGCTAGTTGTTGTTGAGACAGAGGAGATGTACAAGGCCCGCAAAGAAAAGGAGGAAGCCCACCGAGCTCTTCGTCCTCCGTTGGTCATAAACCATCAACCTGCTTCTTCTGCTTAG

Protein sequence

>MS.gene36543.t1

MSMNAFKAFKSCVPIAYSPNLYITLVRGIPGTRKQHRRTLEALRLGKCNRTVMRWNTPTVRGMIQQVKRLVVVETEEMYKARKEKEEAHRALRPPLVINHQPASSA