Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36838.t1 | XP_004510012.1 | 90.7 | 215 | 20 | 0 | 1 | 215 | 84 | 298 | 7.90E-108 | 399.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36838.t1 | Q64LD2 | 34.2 | 199 | 124 | 3 | 21 | 215 | 227 | 422 | 2.4e-28 | 127.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36838.t1 | A0A1S2YU82 | 90.7 | 215 | 20 | 0 | 1 | 215 | 84 | 298 | 5.7e-108 | 399.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049850 | MS.gene36838 | 0.805595 | 1.24E-49 | -1.69E-46 |
| MS.gene050303 | MS.gene36838 | 0.802245 | 6.15E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene36838 | 0.807618 | 4.63E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene36838 | 0.804367 | 2.24E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene36838 | 0.80713 | 5.88E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene36838 | 0.804549 | 2.05E-49 | -1.69E-46 |
| MS.gene058829 | MS.gene36838 | 0.803257 | 3.80E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene36838 | 0.810249 | 1.27E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTGCTGGGATGGACAATT+TGG | 0.150720 | 8.4:-43950517 | MS.gene36838:CDS |
| AATTCAAATATCTTCCTTTC+TGG | 0.153427 | 8.4:-43950276 | MS.gene36838:CDS |
| CATAACTATAATCGAAATTT+AGG | 0.181559 | 8.4:-43950201 | MS.gene36838:CDS |
| AGGAAGATATTTGAATTATC+TGG | 0.183670 | 8.4:+43950282 | None:intergenic |
| GCTCACCTCCTTGCCTCTGC+TGG | 0.219959 | 8.4:-43950531 | MS.gene36838:CDS |
| TCAATAATGCTAAAATGTTA+TGG | 0.268196 | 8.4:+43950910 | None:intergenic |
| CAAAGGGACAAATCAAACTA+TGG | 0.288706 | 8.4:-43950248 | MS.gene36838:CDS |
| AAGCTGTCTGCAACTCTATC+TGG | 0.297095 | 8.4:-43950843 | MS.gene36838:CDS |
| CAATAATGCTAAAATGTTAT+GGG | 0.304689 | 8.4:+43950911 | None:intergenic |
| CAAACACCGAGTTCAGGTTT+TGG | 0.328754 | 8.4:+43950868 | None:intergenic |
| GTGAGAATTCTATTATCGTT+TGG | 0.344806 | 8.4:-43950101 | MS.gene36838:CDS |
| TGAGAATTCTATTATCGTTT+GGG | 0.353439 | 8.4:-43950100 | MS.gene36838:CDS |
| ATTCAAATATCTTCCTTTCT+GGG | 0.354275 | 8.4:-43950275 | MS.gene36838:CDS |
| ATTTCTTCTAGTGACGTATC+TGG | 0.385677 | 8.4:-43950135 | MS.gene36838:CDS |
| TGGACAATTTGGTTTGCATA+TGG | 0.393563 | 8.4:-43950506 | MS.gene36838:CDS |
| CTCACCTCCTTGCCTCTGCT+GGG | 0.394833 | 8.4:-43950530 | MS.gene36838:CDS |
| CCTCCTTGCCTCTGCTGGGA+TGG | 0.398726 | 8.4:-43950526 | MS.gene36838:CDS |
| CAGCAGTGAAAGATGTAAAA+TGG | 0.401012 | 8.4:-43950431 | MS.gene36838:CDS |
| ACAAGTACCGTTGTCTAATC+AGG | 0.413649 | 8.4:-43950067 | MS.gene36838:CDS |
| AGGTTGGTTGATGTTGAAAA+GGG | 0.433049 | 8.4:-43950360 | MS.gene36838:CDS |
| GGAAGATATTTGAATTATCT+GGG | 0.436810 | 8.4:+43950283 | None:intergenic |
| AAGGTTGGTTGATGTTGAAA+AGG | 0.438941 | 8.4:-43950361 | MS.gene36838:CDS |
| ATACATTGGTCATCAACTAA+TGG | 0.444550 | 8.4:-43950798 | MS.gene36838:intron |
| TGGTTTGCATATGGAACGTA+TGG | 0.473476 | 8.4:-43950497 | MS.gene36838:CDS |
| TTCAAAGAAGATCAAATGGC+TGG | 0.481015 | 8.4:-43950321 | MS.gene36838:CDS |
| GGGAACTTCTTGCTTTCTTG+TGG | 0.481727 | 8.4:-43950399 | MS.gene36838:CDS |
| TTCAAATATCTTCCTTTCTG+GGG | 0.504458 | 8.4:-43950274 | MS.gene36838:CDS |
| TGAAAAGGGGATGGAAACTC+AGG | 0.548181 | 8.4:-43950346 | MS.gene36838:CDS |
| AAAGGGACAAATCAAACTAT+GGG | 0.552104 | 8.4:-43950247 | MS.gene36838:CDS |
| TATAAATATAGGGAGTATCT+CGG | 0.557018 | 8.4:-43950946 | None:intergenic |
| TCAAATATCTTCCTTTCTGG+GGG | 0.557329 | 8.4:-43950273 | MS.gene36838:CDS |
| GGTTGATGTTGAAAAGGGGA+TGG | 0.571477 | 8.4:-43950355 | MS.gene36838:CDS |
| CATCTTTCACTGCTGCATTG+TGG | 0.587898 | 8.4:+43950439 | None:intergenic |
| GTGGATATGATTGCACATCA+AGG | 0.600456 | 8.4:-43950380 | MS.gene36838:CDS |
| TGTCCATCCCAGCAGAGGCA+AGG | 0.605709 | 8.4:+43950523 | None:intergenic |
| CAAATTGTCCATCCCAGCAG+AGG | 0.614450 | 8.4:+43950518 | None:intergenic |
| GAGCTGTCAATGCTATACAT+TGG | 0.616281 | 8.4:-43950812 | MS.gene36838:CDS |
| ATATGATTGCACATCAAGGT+TGG | 0.628197 | 8.4:-43950376 | MS.gene36838:CDS |
| GATGTAAAATGGTCTCAACA+AGG | 0.630392 | 8.4:-43950420 | MS.gene36838:CDS |
| ATGTAAAATGGTCTCAACAA+GGG | 0.656310 | 8.4:-43950419 | MS.gene36838:CDS |
| GAGTCACCAAAACCTGAACT+CGG | 0.656319 | 8.4:-43950874 | MS.gene36838:CDS |
| GGTTGGTTGATGTTGAAAAG+GGG | 0.664123 | 8.4:-43950359 | MS.gene36838:CDS |
| CCATCCCAGCAGAGGCAAGG+AGG | 0.679470 | 8.4:+43950526 | None:intergenic |
| AGATATTTGAATTATCTGGG+TGG | 0.709902 | 8.4:+43950286 | None:intergenic |
| TATCGTACCTGATTAGACAA+CGG | 0.715508 | 8.4:+43950060 | None:intergenic |
| AAATATAGGGAGTATCTCGG+AGG | 0.740361 | 8.4:-43950943 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTTGAGTTTACAAAAAAT+GGG | - | chr8.4:43950827-43950846 | MS.gene36838:CDS | 20.0% |
| !! | CAATAATGCTAAAATGTTAT+GGG | + | chr8.4:43950086-43950105 | None:intergenic | 20.0% |
| !! | CATAACTATAATCGAAATTT+AGG | - | chr8.4:43950793-43950812 | MS.gene36838:intron | 20.0% |
| !! | TCAATAATGCTAAAATGTTA+TGG | + | chr8.4:43950087-43950106 | None:intergenic | 20.0% |
| !!! | ATACAACTTTTAGGAAATAA+TGG | - | chr8.4:43950258-43950277 | MS.gene36838:CDS | 20.0% |
| ! | AAAAGTTGTATCACAAACAA+GGG | + | chr8.4:43950249-43950268 | None:intergenic | 25.0% |
| ! | AATTCAAATATCTTCCTTTC+TGG | - | chr8.4:43950718-43950737 | MS.gene36838:intron | 25.0% |
| ! | AGGAAGATATTTGAATTATC+TGG | + | chr8.4:43950715-43950734 | None:intergenic | 25.0% |
| ! | ATTCAAATATCTTCCTTTCT+GGG | - | chr8.4:43950719-43950738 | MS.gene36838:intron | 25.0% |
| ! | GATGTTGAGTTTACAAAAAA+TGG | - | chr8.4:43950826-43950845 | MS.gene36838:CDS | 25.0% |
| ! | GGAAGATATTTGAATTATCT+GGG | + | chr8.4:43950714-43950733 | None:intergenic | 25.0% |
| ! | TAAAAGTTGTATCACAAACA+AGG | + | chr8.4:43950250-43950269 | None:intergenic | 25.0% |
| ! | TACTAATAACATATACAGCA+AGG | + | chr8.4:43950415-43950434 | None:intergenic | 25.0% |
| ! | TGAGAATTCTATTATCGTTT+GGG | - | chr8.4:43950894-43950913 | MS.gene36838:CDS | 25.0% |
| !! | TTGTTTGTGATACAACTTTT+AGG | - | chr8.4:43950249-43950268 | MS.gene36838:CDS | 25.0% |
| AAAGGGACAAATCAAACTAT+GGG | - | chr8.4:43950747-43950766 | MS.gene36838:intron | 30.0% | |
| AGATATTTGAATTATCTGGG+TGG | + | chr8.4:43950711-43950730 | None:intergenic | 30.0% | |
| ATACATTGGTCATCAACTAA+TGG | - | chr8.4:43950196-43950215 | MS.gene36838:CDS | 30.0% | |
| ATGTAAAATGGTCTCAACAA+GGG | - | chr8.4:43950575-43950594 | MS.gene36838:intron | 30.0% | |
| GTGAGAATTCTATTATCGTT+TGG | - | chr8.4:43950893-43950912 | MS.gene36838:CDS | 30.0% | |
| TTCAAATATCTTCCTTTCTG+GGG | - | chr8.4:43950720-43950739 | MS.gene36838:intron | 30.0% | |
| ! | GGTTTTCAAAGAAGATCAAA+TGG | - | chr8.4:43950669-43950688 | MS.gene36838:intron | 30.0% |
| ATATGATTGCACATCAAGGT+TGG | - | chr8.4:43950618-43950637 | MS.gene36838:intron | 35.0% | |
| ATTTCTTCTAGTGACGTATC+TGG | - | chr8.4:43950859-43950878 | MS.gene36838:CDS | 35.0% | |
| CAAAGGGACAAATCAAACTA+TGG | - | chr8.4:43950746-43950765 | MS.gene36838:intron | 35.0% | |
| CAGCAGTGAAAGATGTAAAA+TGG | - | chr8.4:43950563-43950582 | MS.gene36838:intron | 35.0% | |
| GATGTAAAATGGTCTCAACA+AGG | - | chr8.4:43950574-43950593 | MS.gene36838:intron | 35.0% | |
| TATCGTACCTGATTAGACAA+CGG | + | chr8.4:43950937-43950956 | None:intergenic | 35.0% | |
| TCAAATATCTTCCTTTCTGG+GGG | - | chr8.4:43950721-43950740 | MS.gene36838:intron | 35.0% | |
| TTCAAAGAAGATCAAATGGC+TGG | - | chr8.4:43950673-43950692 | MS.gene36838:intron | 35.0% | |
| TTGTCTAATCAGGTACGATA+GGG | - | chr8.4:43950937-43950956 | MS.gene36838:CDS | 35.0% | |
| ! | AAGGTTGGTTGATGTTGAAA+AGG | - | chr8.4:43950633-43950652 | MS.gene36838:intron | 35.0% |
| ! | AGGTTGGTTGATGTTGAAAA+GGG | - | chr8.4:43950634-43950653 | MS.gene36838:intron | 35.0% |
| !! | TGGACAATTTGGTTTGCATA+TGG | - | chr8.4:43950488-43950507 | MS.gene36838:CDS | 35.0% |
| GAGCTGTCAATGCTATACAT+TGG | - | chr8.4:43950182-43950201 | MS.gene36838:CDS | 40.0% | |
| GTGGATATGATTGCACATCA+AGG | - | chr8.4:43950614-43950633 | MS.gene36838:intron | 40.0% | |
| GTTGTCTAATCAGGTACGAT+AGG | - | chr8.4:43950936-43950955 | MS.gene36838:CDS | 40.0% | |
| ! | ACAAGTACCGTTGTCTAATC+AGG | - | chr8.4:43950927-43950946 | MS.gene36838:CDS | 40.0% |
| ! | GGTTGGTTGATGTTGAAAAG+GGG | - | chr8.4:43950635-43950654 | MS.gene36838:intron | 40.0% |
| ! | TGGTTTGCATATGGAACGTA+TGG | - | chr8.4:43950497-43950516 | MS.gene36838:CDS | 40.0% |
| AAGCTGTCTGCAACTCTATC+TGG | - | chr8.4:43950151-43950170 | MS.gene36838:CDS | 45.0% | |
| CAAGGATATCTCTCTTGCTC+TGG | + | chr8.4:43950397-43950416 | None:intergenic | 45.0% | |
| CATCTTTCACTGCTGCATTG+TGG | + | chr8.4:43950558-43950577 | None:intergenic | 45.0% | |
| CTATCCTCTCCTTCATGTGT+GGG | + | chr8.4:43950373-43950392 | None:intergenic | 45.0% | |
| GAGTCACCAAAACCTGAACT+CGG | - | chr8.4:43950120-43950139 | MS.gene36838:CDS | 45.0% | |
| GGGAACTTCTTGCTTTCTTG+TGG | - | chr8.4:43950595-43950614 | MS.gene36838:intron | 45.0% | |
| TCTATCCTCTCCTTCATGTG+TGG | + | chr8.4:43950374-43950393 | None:intergenic | 45.0% | |
| TGAAAAGGGGATGGAAACTC+AGG | - | chr8.4:43950648-43950667 | MS.gene36838:intron | 45.0% | |
| ! | GCTTTTCAAACACCGAGTTC+AGG | + | chr8.4:43950135-43950154 | None:intergenic | 45.0% |
| ! | GGTTGATGTTGAAAAGGGGA+TGG | - | chr8.4:43950639-43950658 | MS.gene36838:intron | 45.0% |
| ! | TTCCTTTCTGGGGGATCAAA+GGG | - | chr8.4:43950730-43950749 | MS.gene36838:intron | 45.0% |
| !! | CAAACACCGAGTTCAGGTTT+TGG | + | chr8.4:43950129-43950148 | None:intergenic | 45.0% |
| AGTCACACACCCACACATGA+AGG | - | chr8.4:43950361-43950380 | MS.gene36838:CDS | 50.0% | |
| CAAATTGTCCATCCCAGCAG+AGG | + | chr8.4:43950479-43950498 | None:intergenic | 50.0% | |
| CTCTGCTGGGATGGACAATT+TGG | - | chr8.4:43950477-43950496 | MS.gene36838:CDS | 50.0% | |
| ! | CTTCCTTTCTGGGGGATCAA+AGG | - | chr8.4:43950729-43950748 | MS.gene36838:intron | 50.0% |
| CACACCCACACATGAAGGAG+AGG | - | chr8.4:43950366-43950385 | MS.gene36838:CDS | 55.0% | |
| GTCCCTTTGATCCCCCAGAA+AGG | + | chr8.4:43950735-43950754 | None:intergenic | 55.0% | |
| AGGCAAGGAGGTGAGCTGCA+AGG | + | chr8.4:43950459-43950478 | None:intergenic | 60.0% | |
| CTCACCTCCTTGCCTCTGCT+GGG | - | chr8.4:43950464-43950483 | MS.gene36838:CDS | 60.0% | |
| TGTCCATCCCAGCAGAGGCA+AGG | + | chr8.4:43950474-43950493 | None:intergenic | 60.0% | |
| CCATCCCAGCAGAGGCAAGG+AGG | + | chr8.4:43950471-43950490 | None:intergenic | 65.0% | |
| CCTCCTTGCCTCTGCTGGGA+TGG | - | chr8.4:43950468-43950487 | MS.gene36838:CDS | 65.0% | |
| GCTCACCTCCTTGCCTCTGC+TGG | - | chr8.4:43950463-43950482 | MS.gene36838:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 43950059 | 43950957 | 43950059 | ID=MS.gene36838 |
| chr8.4 | mRNA | 43950059 | 43950957 | 43950059 | ID=MS.gene36838.t1;Parent=MS.gene36838 |
| chr8.4 | exon | 43950799 | 43950957 | 43950799 | ID=MS.gene36838.t1.exon1;Parent=MS.gene36838.t1 |
| chr8.4 | CDS | 43950799 | 43950957 | 43950799 | ID=cds.MS.gene36838.t1;Parent=MS.gene36838.t1 |
| chr8.4 | exon | 43950059 | 43950552 | 43950059 | ID=MS.gene36838.t1.exon2;Parent=MS.gene36838.t1 |
| chr8.4 | CDS | 43950059 | 43950552 | 43950059 | ID=cds.MS.gene36838.t1;Parent=MS.gene36838.t1 |
| Gene Sequence |
| Protein sequence |
