Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36868.t1 | XP_013444918.1 | 90.2 | 184 | 18 | 0 | 1 | 184 | 1 | 184 | 2.60E-91 | 344.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36868.t1 | Q58FX0 | 34.1 | 135 | 72 | 5 | 6 | 131 | 8 | 134 | 8.9e-08 | 58.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36868.t1 | I3S8A1 | 90.2 | 184 | 18 | 0 | 1 | 184 | 1 | 184 | 1.9e-91 | 344.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene36868 | 0.805418 | 1.35E-49 | -1.69E-46 |
| MS.gene051226 | MS.gene36868 | 0.829354 | 5.39E-55 | -1.69E-46 |
| MS.gene054892 | MS.gene36868 | 0.846596 | 1.97E-59 | -1.69E-46 |
| MS.gene054893 | MS.gene36868 | 0.853448 | 2.37E-61 | -1.69E-46 |
| MS.gene057093 | MS.gene36868 | 0.808007 | 3.83E-50 | -1.69E-46 |
| MS.gene057174 | MS.gene36868 | 0.80292 | 4.46E-49 | -1.69E-46 |
| MS.gene057622 | MS.gene36868 | 0.828758 | 7.52E-55 | -1.69E-46 |
| MS.gene06023 | MS.gene36868 | 0.821844 | 3.25E-53 | -1.69E-46 |
| MS.gene060412 | MS.gene36868 | 0.805954 | 1.04E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36868.t1 | MTR_8g432470 | 90.217 | 184 | 18 | 0 | 1 | 184 | 1 | 184 | 3.51e-123 | 345 |
| MS.gene36868.t1 | MTR_8g432440 | 88.108 | 185 | 20 | 2 | 1 | 184 | 1 | 184 | 3.31e-117 | 330 |
| MS.gene36868.t1 | MTR_8g432420 | 88.108 | 185 | 20 | 2 | 1 | 184 | 1 | 184 | 3.31e-117 | 330 |
| MS.gene36868.t1 | MTR_8g432460 | 86.957 | 184 | 23 | 1 | 1 | 184 | 1 | 183 | 4.11e-117 | 330 |
| MS.gene36868.t1 | MTR_8g432450 | 89.831 | 177 | 17 | 1 | 1 | 176 | 1 | 177 | 7.86e-115 | 323 |
| MS.gene36868.t1 | MTR_8g432490 | 84.239 | 184 | 28 | 1 | 1 | 184 | 1 | 183 | 1.64e-112 | 318 |
| MS.gene36868.t1 | MTR_3g064780 | 81.215 | 181 | 33 | 1 | 1 | 181 | 1 | 180 | 1.52e-106 | 305 |
| MS.gene36868.t1 | MTR_8g432480 | 52.222 | 180 | 57 | 6 | 5 | 175 | 7 | 166 | 1.76e-49 | 158 |
| MS.gene36868.t1 | MTR_7g076000 | 36.264 | 182 | 83 | 8 | 5 | 177 | 2 | 159 | 1.38e-25 | 97.1 |
| MS.gene36868.t1 | MTR_8g432400 | 36.559 | 186 | 96 | 8 | 5 | 180 | 2 | 175 | 2.03e-22 | 89.4 |
| MS.gene36868.t1 | MTR_8g432370 | 36.757 | 185 | 96 | 7 | 5 | 180 | 2 | 174 | 1.65e-21 | 86.7 |
| MS.gene36868.t1 | MTR_8g432390 | 37.634 | 186 | 94 | 8 | 5 | 180 | 2 | 175 | 8.23e-21 | 85.1 |
| MS.gene36868.t1 | MTR_8g432350 | 36.612 | 183 | 95 | 7 | 5 | 178 | 2 | 172 | 1.86e-20 | 84.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36868.t1 | AT2G45760 | 34.074 | 135 | 72 | 6 | 6 | 131 | 8 | 134 | 2.13e-11 | 60.5 |
Find 51 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTATGGTGGTCGATTAAC+TGG | 0.276699 | 8.4:-64126889 | MS.gene36868:CDS |
| TTCAAAAGTGTTCGTCCTAT+TGG | 0.279263 | 8.4:-64127102 | MS.gene36868:CDS |
| TCAAATCTTTAAATAATGTT+TGG | 0.280513 | 8.4:+64126802 | None:intergenic |
| AAAATTCGAGTTTCCTTTCA+TGG | 0.287575 | 8.4:-64127194 | MS.gene36868:CDS |
| TCAAAAGTGTTCGTCCTATT+GGG | 0.298427 | 8.4:-64127101 | MS.gene36868:CDS |
| TTGCTATTGGTATTCCCTTT+TGG | 0.313784 | 8.4:-64126834 | MS.gene36868:CDS |
| CAAATCTTTAAATAATGTTT+GGG | 0.339025 | 8.4:+64126803 | None:intergenic |
| TAATTGTTACACGACTAAAA+TGG | 0.339799 | 8.4:-64127244 | MS.gene36868:CDS |
| TGAGTTATAAATTGAGGGAT+TGG | 0.355220 | 8.4:-64127014 | MS.gene36868:CDS |
| AACTCTAATGGTGTTGCTAT+TGG | 0.365765 | 8.4:-64126847 | MS.gene36868:CDS |
| GATTGGGAAGGGAAGCAAAA+TGG | 0.374296 | 8.4:-64126997 | MS.gene36868:CDS |
| GCAATTCTTGCCACCCCAAT+AGG | 0.383363 | 8.4:+64127087 | None:intergenic |
| GTTTGGGTAATTCCACCAAA+AGG | 0.410769 | 8.4:+64126819 | None:intergenic |
| GGTGGCAAGAATTGCCGTTT+CGG | 0.415973 | 8.4:-64127079 | MS.gene36868:CDS |
| CAGTTTGAGGGTGGTTGTGC+CGG | 0.417940 | 8.4:-64126962 | MS.gene36868:CDS |
| AGTTATTCATTTCAGTTTGA+GGG | 0.452076 | 8.4:-64126974 | MS.gene36868:CDS |
| GAGTTATAAATTGAGGGATT+GGG | 0.478191 | 8.4:-64127013 | MS.gene36868:CDS |
| AAAACTTCTCTTTCCATGAA+AGG | 0.488634 | 8.4:+64127181 | None:intergenic |
| TTTGGGTAATTCCACCAAAA+GGG | 0.494809 | 8.4:+64126820 | None:intergenic |
| GAGTTATTCATTTCAGTTTG+AGG | 0.495462 | 8.4:-64126975 | MS.gene36868:CDS |
| GATGCATATGTCGTCGTTCG+AGG | 0.506231 | 8.4:-64127276 | MS.gene36868:CDS |
| AAAGATAACGACGATAATTG+TGG | 0.511679 | 8.4:-64127219 | MS.gene36868:CDS |
| AAGATAACGACGATAATTGT+GGG | 0.511918 | 8.4:-64127218 | MS.gene36868:CDS |
| TTAGAGATCACGGTTATATC+CGG | 0.513948 | 8.4:-64127321 | MS.gene36868:CDS |
| TTAATTCTCTACTATGGGTG+TGG | 0.519159 | 8.4:-64127355 | None:intergenic |
| CCCGTTTAAAATATCCGAAA+CGG | 0.521654 | 8.4:+64127065 | None:intergenic |
| TGCAAGGTCAATCACGTTTG+AGG | 0.523501 | 8.4:-64127139 | MS.gene36868:CDS |
| GTGGTGCATGGAAAGAATTA+TGG | 0.540917 | 8.4:-64126904 | MS.gene36868:CDS |
| GAGAAGCAAACGGTGGTGCA+TGG | 0.551670 | 8.4:-64126916 | MS.gene36868:CDS |
| GTTGGTGTCAAGAACTCTAA+TGG | 0.557347 | 8.4:-64126859 | MS.gene36868:CDS |
| TATTCATTTCAGTTTGAGGG+TGG | 0.561056 | 8.4:-64126971 | MS.gene36868:CDS |
| CAAAAGTGTTCGTCCTATTG+GGG | 0.562181 | 8.4:-64127100 | MS.gene36868:CDS |
| ATAAATTGAGGGATTGGGAA+GGG | 0.564987 | 8.4:-64127008 | MS.gene36868:CDS |
| TGAACATGCCATTGCATGCA+AGG | 0.566598 | 8.4:-64127155 | MS.gene36868:CDS |
| GGTTGTGCCGGAGCAAAGAT+CGG | 0.574606 | 8.4:-64126950 | MS.gene36868:CDS |
| TGGTGGTCGATTAACTGGAA+TGG | 0.578834 | 8.4:-64126884 | MS.gene36868:CDS |
| CGATTAACTGGAATGGATGT+TGG | 0.583378 | 8.4:-64126877 | MS.gene36868:CDS |
| AACGACGACATATGCATCCT+CGG | 0.584005 | 8.4:+64127281 | None:intergenic |
| ACGGTAACCGATCTTTGCTC+CGG | 0.597422 | 8.4:+64126943 | None:intergenic |
| TATAAATTGAGGGATTGGGA+AGG | 0.601127 | 8.4:-64127009 | MS.gene36868:CDS |
| CTCACGTACATTAGAGATCA+CGG | 0.606187 | 8.4:-64127331 | MS.gene36868:CDS |
| GTGATTGACCTTGCATGCAA+TGG | 0.618595 | 8.4:+64127147 | None:intergenic |
| GCAAAGATCGGTTACCGTGG+TGG | 0.620546 | 8.4:-64126938 | MS.gene36868:CDS |
| TACCGTGGTGGAGAAGCAAA+CGG | 0.621907 | 8.4:-64126926 | MS.gene36868:CDS |
| CACCGTTTGCTTCTCCACCA+CGG | 0.628730 | 8.4:+64126924 | None:intergenic |
| AAGTGTTCGTCCTATTGGGG+TGG | 0.646267 | 8.4:-64127097 | MS.gene36868:CDS |
| CGTGATCTCTAATGTACGTG+AGG | 0.648406 | 8.4:+64127332 | None:intergenic |
| CGAAAATATTCATGTAACCG+AGG | 0.661605 | 8.4:-64127298 | MS.gene36868:CDS |
| GGAGCAAAGATCGGTTACCG+TGG | 0.687601 | 8.4:-64126941 | MS.gene36868:CDS |
| GTGCATGGAAAGAATTATGG+TGG | 0.715501 | 8.4:-64126901 | MS.gene36868:CDS |
| CGTGGTGGAGAAGCAAACGG+TGG | 0.754469 | 8.4:-64126923 | MS.gene36868:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTTGAGTTATAAATTG+AGG | - | chr8.4:64127132-64127151 | MS.gene36868:CDS | 15.0% |
| !!! | AATTTTGAGTTATAAATTGA+GGG | - | chr8.4:64127133-64127152 | MS.gene36868:CDS | 15.0% |
| ! | AGTTATTCATTTCAGTTTGA+GGG | - | chr8.4:64127178-64127197 | MS.gene36868:CDS | 25.0% |
| ! | TAATTGTTACACGACTAAAA+TGG | - | chr8.4:64126908-64126927 | MS.gene36868:CDS | 25.0% |
| AAAACTTCTCTTTCCATGAA+AGG | + | chr8.4:64126974-64126993 | None:intergenic | 30.0% | |
| AAAGATAACGACGATAATTG+TGG | - | chr8.4:64126933-64126952 | MS.gene36868:CDS | 30.0% | |
| AAGATAACGACGATAATTGT+GGG | - | chr8.4:64126934-64126953 | MS.gene36868:CDS | 30.0% | |
| GAGTTATAAATTGAGGGATT+GGG | - | chr8.4:64127139-64127158 | MS.gene36868:CDS | 30.0% | |
| GAGTTATTCATTTCAGTTTG+AGG | - | chr8.4:64127177-64127196 | MS.gene36868:CDS | 30.0% | |
| TGAGTTATAAATTGAGGGAT+TGG | - | chr8.4:64127138-64127157 | MS.gene36868:CDS | 30.0% | |
| ! | AAAATTCGAGTTTCCTTTCA+TGG | - | chr8.4:64126958-64126977 | MS.gene36868:CDS | 30.0% |
| !! | TATTTTAAACGGGGATGAAT+CGG | - | chr8.4:64127097-64127116 | MS.gene36868:CDS | 30.0% |
| AATTATGGTGGTCGATTAAC+TGG | - | chr8.4:64127263-64127282 | MS.gene36868:CDS | 35.0% | |
| ATAAATTGAGGGATTGGGAA+GGG | - | chr8.4:64127144-64127163 | MS.gene36868:CDS | 35.0% | |
| CGAAAATATTCATGTAACCG+AGG | - | chr8.4:64126854-64126873 | MS.gene36868:CDS | 35.0% | |
| TATAAATTGAGGGATTGGGA+AGG | - | chr8.4:64127143-64127162 | MS.gene36868:CDS | 35.0% | |
| TATTCATTTCAGTTTGAGGG+TGG | - | chr8.4:64127181-64127200 | MS.gene36868:CDS | 35.0% | |
| TTAGAGATCACGGTTATATC+CGG | - | chr8.4:64126831-64126850 | MS.gene36868:CDS | 35.0% | |
| TTTGGGTAATTCCACCAAAA+GGG | + | chr8.4:64127335-64127354 | None:intergenic | 35.0% | |
| ! | CCCGTTTAAAATATCCGAAA+CGG | + | chr8.4:64127090-64127109 | None:intergenic | 35.0% |
| ! | GGTTACATGAATATTTTCGC+CGG | + | chr8.4:64126853-64126872 | None:intergenic | 35.0% |
| !! | AACTCTAATGGTGTTGCTAT+TGG | - | chr8.4:64127305-64127324 | MS.gene36868:CDS | 35.0% |
| !! | CCGTTTCGGATATTTTAAAC+GGG | - | chr8.4:64127087-64127106 | MS.gene36868:CDS | 35.0% |
| !! | CGTTTCGGATATTTTAAACG+GGG | - | chr8.4:64127088-64127107 | MS.gene36868:CDS | 35.0% |
| !! | GCCGTTTCGGATATTTTAAA+CGG | - | chr8.4:64127086-64127105 | MS.gene36868:CDS | 35.0% |
| !! | TCAAAAGTGTTCGTCCTATT+GGG | - | chr8.4:64127051-64127070 | MS.gene36868:CDS | 35.0% |
| !! | TTCAAAAGTGTTCGTCCTAT+TGG | - | chr8.4:64127050-64127069 | MS.gene36868:CDS | 35.0% |
| !! | TTGCTATTGGTATTCCCTTT+TGG | - | chr8.4:64127318-64127337 | MS.gene36868:CDS | 35.0% |
| CGATTAACTGGAATGGATGT+TGG | - | chr8.4:64127275-64127294 | MS.gene36868:CDS | 40.0% | |
| CTCACGTACATTAGAGATCA+CGG | - | chr8.4:64126821-64126840 | MS.gene36868:CDS | 40.0% | |
| GTGCATGGAAAGAATTATGG+TGG | - | chr8.4:64127251-64127270 | MS.gene36868:CDS | 40.0% | |
| GTGGTGCATGGAAAGAATTA+TGG | - | chr8.4:64127248-64127267 | MS.gene36868:CDS | 40.0% | |
| GTTTGGGTAATTCCACCAAA+AGG | + | chr8.4:64127336-64127355 | None:intergenic | 40.0% | |
| ! | CTATTGGTATTCCCTTTTGG+TGG | - | chr8.4:64127321-64127340 | MS.gene36868:CDS | 40.0% |
| !! | CAAAAGTGTTCGTCCTATTG+GGG | - | chr8.4:64127052-64127071 | MS.gene36868:CDS | 40.0% |
| !! | GTTGGTGTCAAGAACTCTAA+TGG | - | chr8.4:64127293-64127312 | MS.gene36868:CDS | 40.0% |
| AACGACGACATATGCATCCT+CGG | + | chr8.4:64126874-64126893 | None:intergenic | 45.0% | |
| CGTGATCTCTAATGTACGTG+AGG | + | chr8.4:64126823-64126842 | None:intergenic | 45.0% | |
| GATTGGGAAGGGAAGCAAAA+TGG | - | chr8.4:64127155-64127174 | MS.gene36868:CDS | 45.0% | |
| GTGATTGACCTTGCATGCAA+TGG | + | chr8.4:64127008-64127027 | None:intergenic | 45.0% | |
| TGAACATGCCATTGCATGCA+AGG | - | chr8.4:64126997-64127016 | MS.gene36868:CDS | 45.0% | |
| TGCAAGGTCAATCACGTTTG+AGG | - | chr8.4:64127013-64127032 | MS.gene36868:CDS | 45.0% | |
| TGGTGGTCGATTAACTGGAA+TGG | - | chr8.4:64127268-64127287 | MS.gene36868:CDS | 45.0% | |
| AAGTGTTCGTCCTATTGGGG+TGG | - | chr8.4:64127055-64127074 | MS.gene36868:CDS | 50.0% | |
| ACGGTAACCGATCTTTGCTC+CGG | + | chr8.4:64127212-64127231 | None:intergenic | 50.0% | |
| GATGCATATGTCGTCGTTCG+AGG | - | chr8.4:64126876-64126895 | MS.gene36868:CDS | 50.0% | |
| GCAATTCTTGCCACCCCAAT+AGG | + | chr8.4:64127068-64127087 | None:intergenic | 50.0% | |
| ! | GGTGGCAAGAATTGCCGTTT+CGG | - | chr8.4:64127073-64127092 | MS.gene36868:CDS | 50.0% |
| ! | TACCGTGGTGGAGAAGCAAA+CGG | - | chr8.4:64127226-64127245 | MS.gene36868:CDS | 50.0% |
| CACCGTTTGCTTCTCCACCA+CGG | + | chr8.4:64127231-64127250 | None:intergenic | 55.0% | |
| CAGTTTGAGGGTGGTTGTGC+CGG | - | chr8.4:64127190-64127209 | MS.gene36868:CDS | 55.0% | |
| GAGAAGCAAACGGTGGTGCA+TGG | - | chr8.4:64127236-64127255 | MS.gene36868:CDS | 55.0% | |
| GCAAAGATCGGTTACCGTGG+TGG | - | chr8.4:64127214-64127233 | MS.gene36868:CDS | 55.0% | |
| GGAGCAAAGATCGGTTACCG+TGG | - | chr8.4:64127211-64127230 | MS.gene36868:CDS | 55.0% | |
| GGTTGTGCCGGAGCAAAGAT+CGG | - | chr8.4:64127202-64127221 | MS.gene36868:CDS | 55.0% | |
| ! | CGTGGTGGAGAAGCAAACGG+TGG | - | chr8.4:64127229-64127248 | MS.gene36868:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 64126810 | 64127364 | 64126810 | ID=MS.gene36868 |
| chr8.4 | mRNA | 64126810 | 64127364 | 64126810 | ID=MS.gene36868.t1;Parent=MS.gene36868 |
| chr8.4 | exon | 64126810 | 64127364 | 64126810 | ID=MS.gene36868.t1.exon1;Parent=MS.gene36868.t1 |
| chr8.4 | CDS | 64126810 | 64127364 | 64126810 | ID=cds.MS.gene36868.t1;Parent=MS.gene36868.t1 |
| Gene Sequence |
| Protein sequence |
