Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36896.t1 | RHN40044.1 | 67.3 | 150 | 30 | 4 | 1 | 146 | 1 | 135 | 9.90E-46 | 193 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36896.t1 | Q7M443 | 38.2 | 136 | 65 | 5 | 14 | 145 | 2 | 122 | 5.6e-13 | 75.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36896.t1 | A0A396GKD7 | 67.3 | 150 | 30 | 4 | 1 | 146 | 1 | 135 | 7.2e-46 | 193.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052187 | MS.gene36896 | 0.834444 | 2.99E-56 | -1.69E-46 |
| MS.gene053074 | MS.gene36896 | 0.813764 | 2.17E-51 | -1.69E-46 |
| MS.gene05468 | MS.gene36896 | 0.811612 | 6.42E-51 | -1.69E-46 |
| MS.gene055359 | MS.gene36896 | 0.825731 | 4.00E-54 | -1.69E-46 |
| MS.gene055860 | MS.gene36896 | 0.811129 | 8.17E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene36896 | 0.81986 | 9.31E-53 | -1.69E-46 |
| MS.gene059124 | MS.gene36896 | 0.808197 | 3.49E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene36896.t1 | MTR_0002s0040 | 51.282 | 156 | 50 | 4 | 1 | 145 | 1 | 141 | 6.12e-40 | 135 |
| MS.gene36896.t1 | MTR_0263s0020 | 51.282 | 156 | 50 | 4 | 1 | 145 | 1 | 141 | 7.05e-40 | 135 |
| MS.gene36896.t1 | MTR_0002s1060 | 51.299 | 154 | 48 | 6 | 4 | 146 | 6 | 143 | 2.37e-32 | 116 |
| MS.gene36896.t1 | MTR_0062s0090 | 42.254 | 142 | 59 | 3 | 14 | 146 | 4 | 131 | 6.41e-24 | 94.4 |
| MS.gene36896.t1 | MTR_0062s0040 | 42.254 | 142 | 59 | 3 | 14 | 146 | 4 | 131 | 6.41e-24 | 94.4 |
| MS.gene36896.t1 | MTR_0062s0060 | 42.254 | 142 | 59 | 3 | 14 | 146 | 4 | 131 | 6.41e-24 | 94.4 |
| MS.gene36896.t1 | MTR_1457s0010 | 59.524 | 84 | 17 | 2 | 65 | 146 | 1 | 69 | 3.92e-23 | 90.5 |
| MS.gene36896.t1 | MTR_0443s0040 | 42.969 | 128 | 54 | 5 | 14 | 138 | 16 | 127 | 4.68e-21 | 87.0 |
| MS.gene36896.t1 | MTR_0062s0020 | 40.559 | 143 | 60 | 3 | 14 | 146 | 4 | 131 | 1.14e-20 | 85.9 |
| MS.gene36896.t1 | MTR_0062s0170 | 38.732 | 142 | 68 | 4 | 1 | 138 | 1 | 127 | 3.41e-19 | 82.0 |
| MS.gene36896.t1 | MTR_0062s0110 | 38.732 | 142 | 68 | 4 | 1 | 138 | 1 | 127 | 3.41e-19 | 82.0 |
| MS.gene36896.t1 | MTR_0062s0130 | 38.732 | 142 | 68 | 4 | 1 | 138 | 1 | 127 | 3.41e-19 | 82.0 |
| MS.gene36896.t1 | MTR_2g036240 | 38.732 | 142 | 68 | 4 | 1 | 138 | 1 | 127 | 3.41e-19 | 82.0 |
| MS.gene36896.t1 | MTR_2g036260 | 38.732 | 142 | 68 | 4 | 1 | 138 | 1 | 127 | 3.41e-19 | 82.0 |
| MS.gene36896.t1 | MTR_2g036290 | 38.732 | 142 | 68 | 4 | 1 | 138 | 1 | 127 | 3.41e-19 | 82.0 |
| MS.gene36896.t1 | MTR_8g046730 | 38.732 | 142 | 68 | 4 | 1 | 138 | 1 | 127 | 3.41e-19 | 82.0 |
| MS.gene36896.t1 | MTR_6g079630 | 37.226 | 137 | 69 | 4 | 12 | 145 | 32 | 154 | 1.56e-17 | 77.4 |
| MS.gene36896.t1 | MTR_2g096030 | 37.037 | 135 | 68 | 5 | 14 | 145 | 31 | 151 | 6.41e-15 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGGGAATATAAAAGATTT+TGG | 0.113401 | 8.4:+63386873 | None:intergenic |
| AATCGATTTCCATGTCATTT+TGG | 0.188672 | 8.4:-63386835 | MS.gene36896:CDS |
| GGAGAGAAAGGATATTTAGT+AGG | 0.309124 | 8.4:+63386618 | None:intergenic |
| ATCCAAGACTACACGTTTGA+TGG | 0.357506 | 8.4:-63386543 | MS.gene36896:CDS |
| GAGAGAAAGGATATTTAGTA+GGG | 0.368934 | 8.4:+63386619 | None:intergenic |
| GAATCGATCAACAGTGTTAC+CGG | 0.376698 | 8.4:+63386929 | None:intergenic |
| CATCTTCGTTGTAAGTCTCA+TGG | 0.421031 | 8.4:+63386805 | None:intergenic |
| CGGAAAATTCTATGCCGATT+GGG | 0.474111 | 8.4:-63386772 | MS.gene36896:CDS |
| TGCCATCAAACGTGTAGTCT+TGG | 0.476476 | 8.4:+63386541 | None:intergenic |
| CTAAGTACCACCTTGACCTT+GGG | 0.479326 | 8.4:+63386699 | None:intergenic |
| CTGCGGTATACCACGTGTGC+AGG | 0.504725 | 8.4:-63386739 | MS.gene36896:CDS |
| CCGGAAAATTCTATGCCGAT+TGG | 0.509755 | 8.4:-63386773 | MS.gene36896:CDS |
| GCTAAGTACCACCTTGACCT+TGG | 0.518197 | 8.4:+63386698 | None:intergenic |
| CATGAGACTTACAACGAAGA+TGG | 0.533162 | 8.4:-63386804 | MS.gene36896:CDS |
| ATCTTCGTTGTAAGTCTCAT+GGG | 0.537135 | 8.4:+63386806 | None:intergenic |
| GAGAAAGGATATTTAGTAGG+GGG | 0.545897 | 8.4:+63386621 | None:intergenic |
| ACTTACAACGAAGATGGAAA+AGG | 0.547716 | 8.4:-63386798 | MS.gene36896:CDS |
| GCAGATATCAAGATCCCAAT+CGG | 0.555323 | 8.4:+63386758 | None:intergenic |
| GTCAAGGTGGTACTTAGCAT+TGG | 0.558729 | 8.4:-63386693 | MS.gene36896:CDS |
| TCGTGATATACAAAGTGTGC+AGG | 0.567577 | 8.4:-63386667 | MS.gene36896:CDS |
| AAGGTGGTACTTAGCATTGG+TGG | 0.572579 | 8.4:-63386690 | MS.gene36896:CDS |
| AACGAAGATGGAAAAGGCAC+CGG | 0.582283 | 8.4:-63386792 | MS.gene36896:CDS |
| AGAGAAAGGATATTTAGTAG+GGG | 0.590103 | 8.4:+63386620 | None:intergenic |
| GGGCAAAGGCCAAAATGACA+TGG | 0.593006 | 8.4:+63386826 | None:intergenic |
| ACAATGTCTCATCTCATCAC+CGG | 0.607947 | 8.4:-63386948 | MS.gene36896:CDS |
| GTTGTAAGTCTCATGGGCAA+AGG | 0.623453 | 8.4:+63386812 | None:intergenic |
| GCTCAAGTATCCCAAGGTCA+AGG | 0.636705 | 8.4:-63386709 | MS.gene36896:CDS |
| AGCTTGATATCCTGCACACG+TGG | 0.641314 | 8.4:+63386729 | None:intergenic |
| ATCGACGGCATTATCACACC+AGG | 0.643275 | 8.4:+63386581 | None:intergenic |
| TATCAAGCTCAAGTATCCCA+AGG | 0.668476 | 8.4:-63386715 | MS.gene36896:CDS |
| GCAGGATATCATCAAGCACA+AGG | 0.687092 | 8.4:-63386649 | MS.gene36896:CDS |
| CAAGTATCCCAAGGTCAAGG+TGG | 0.692556 | 8.4:-63386706 | MS.gene36896:CDS |
| GATTGGGATCTTGATATCTG+CGG | 0.706223 | 8.4:-63386756 | MS.gene36896:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATAAAAGATTTTGGGTA+TGG | + | chr8.4:63386596-63386615 | None:intergenic | 20.0% |
| !! | CAGGGAATATAAAAGATTTT+GGG | + | chr8.4:63386601-63386620 | None:intergenic | 25.0% |
| !! | TCAGGGAATATAAAAGATTT+TGG | + | chr8.4:63386602-63386621 | None:intergenic | 25.0% |
| !!! | ATTTTGTTGTAGTCAATATC+AGG | + | chr8.4:63386620-63386639 | None:intergenic | 25.0% |
| !!! | TTCATTTTTCGAGAATACAT+TGG | - | chr8.4:63386563-63386582 | MS.gene36896:CDS | 25.0% |
| !!! | TTTTGTTGTAGTCAATATCA+GGG | + | chr8.4:63386619-63386638 | None:intergenic | 25.0% |
| AGAGAAAGGATATTTAGTAG+GGG | + | chr8.4:63386855-63386874 | None:intergenic | 30.0% | |
| GAGAGAAAGGATATTTAGTA+GGG | + | chr8.4:63386856-63386875 | None:intergenic | 30.0% | |
| !! | AATCGATTTCCATGTCATTT+TGG | - | chr8.4:63386637-63386656 | MS.gene36896:CDS | 30.0% |
| !!! | TCTTTTTCAATAGGAGAGAA+AGG | + | chr8.4:63386869-63386888 | None:intergenic | 30.0% |
| ACTTACAACGAAGATGGAAA+AGG | - | chr8.4:63386674-63386693 | MS.gene36896:CDS | 35.0% | |
| ATCTTCGTTGTAAGTCTCAT+GGG | + | chr8.4:63386669-63386688 | None:intergenic | 35.0% | |
| CTCCTATTGAAAAAGAATCC+TGG | - | chr8.4:63386873-63386892 | MS.gene36896:CDS | 35.0% | |
| GAGAAAGGATATTTAGTAGG+GGG | + | chr8.4:63386854-63386873 | None:intergenic | 35.0% | |
| GGAGAGAAAGGATATTTAGT+AGG | + | chr8.4:63386857-63386876 | None:intergenic | 35.0% | |
| !!! | CACCAGGATTCTTTTTCAAT+AGG | + | chr8.4:63386878-63386897 | None:intergenic | 35.0% |
| ATCCAAGACTACACGTTTGA+TGG | - | chr8.4:63386929-63386948 | MS.gene36896:CDS | 40.0% | |
| CATCTTCGTTGTAAGTCTCA+TGG | + | chr8.4:63386670-63386689 | None:intergenic | 40.0% | |
| CATGAGACTTACAACGAAGA+TGG | - | chr8.4:63386668-63386687 | MS.gene36896:CDS | 40.0% | |
| GAATCGATCAACAGTGTTAC+CGG | + | chr8.4:63386546-63386565 | None:intergenic | 40.0% | |
| GATTGGGATCTTGATATCTG+CGG | - | chr8.4:63386716-63386735 | MS.gene36896:CDS | 40.0% | |
| GCAGATATCAAGATCCCAAT+CGG | + | chr8.4:63386717-63386736 | None:intergenic | 40.0% | |
| TATCAAGCTCAAGTATCCCA+AGG | - | chr8.4:63386757-63386776 | MS.gene36896:CDS | 40.0% | |
| TCGTGATATACAAAGTGTGC+AGG | - | chr8.4:63386805-63386824 | MS.gene36896:CDS | 40.0% | |
| ! | CCAATCGGCATAGAATTTTC+CGG | + | chr8.4:63386702-63386721 | None:intergenic | 40.0% |
| ! | CGGAAAATTCTATGCCGATT+GGG | - | chr8.4:63386700-63386719 | MS.gene36896:CDS | 40.0% |
| !! | GATCTTTTTGAGCGAATCGA+CGG | + | chr8.4:63386909-63386928 | None:intergenic | 40.0% |
| CCGGAAAATTCTATGCCGAT+TGG | - | chr8.4:63386699-63386718 | MS.gene36896:CDS | 45.0% | |
| CTAAGTACCACCTTGACCTT+GGG | + | chr8.4:63386776-63386795 | None:intergenic | 45.0% | |
| GCAGGATATCATCAAGCACA+AGG | - | chr8.4:63386823-63386842 | MS.gene36896:CDS | 45.0% | |
| GTCAAGGTGGTACTTAGCAT+TGG | - | chr8.4:63386779-63386798 | MS.gene36896:CDS | 45.0% | |
| GTTGTAAGTCTCATGGGCAA+AGG | + | chr8.4:63386663-63386682 | None:intergenic | 45.0% | |
| TGCCATCAAACGTGTAGTCT+TGG | + | chr8.4:63386934-63386953 | None:intergenic | 45.0% | |
| ! | AACGAAGATGGAAAAGGCAC+CGG | - | chr8.4:63386680-63386699 | MS.gene36896:CDS | 45.0% |
| ! | AAGGTGGTACTTAGCATTGG+TGG | - | chr8.4:63386782-63386801 | MS.gene36896:CDS | 45.0% |
| AGCTTGATATCCTGCACACG+TGG | + | chr8.4:63386746-63386765 | None:intergenic | 50.0% | |
| ATCGACGGCATTATCACACC+AGG | + | chr8.4:63386894-63386913 | None:intergenic | 50.0% | |
| CAAGTATCCCAAGGTCAAGG+TGG | - | chr8.4:63386766-63386785 | MS.gene36896:CDS | 50.0% | |
| GCTAAGTACCACCTTGACCT+TGG | + | chr8.4:63386777-63386796 | None:intergenic | 50.0% | |
| GCTCAAGTATCCCAAGGTCA+AGG | - | chr8.4:63386763-63386782 | MS.gene36896:CDS | 50.0% | |
| GGGCAAAGGCCAAAATGACA+TGG | + | chr8.4:63386649-63386668 | None:intergenic | 50.0% | |
| CTGCGGTATACCACGTGTGC+AGG | - | chr8.4:63386733-63386752 | MS.gene36896:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 63386527 | 63386967 | 63386527 | ID=MS.gene36896 |
| chr8.4 | mRNA | 63386527 | 63386967 | 63386527 | ID=MS.gene36896.t1;Parent=MS.gene36896 |
| chr8.4 | exon | 63386527 | 63386967 | 63386527 | ID=MS.gene36896.t1.exon1;Parent=MS.gene36896.t1 |
| chr8.4 | CDS | 63386527 | 63386967 | 63386527 | ID=cds.MS.gene36896.t1;Parent=MS.gene36896.t1 |
| Gene Sequence |
| Protein sequence |
