Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37396.t1 | XP_024629570.1 | 93.8 | 81 | 5 | 0 | 5 | 85 | 14 | 94 | 2.70E-19 | 104.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37396.t1 | A0A072TLP7 | 91.6 | 83 | 7 | 0 | 3 | 85 | 11 | 93 | 3.3e-19 | 103.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050438 | MS.gene37396 | 0.809442 | 1.89E-50 | -1.69E-46 |
| MS.gene050815 | MS.gene37396 | 0.818372 | 2.03E-52 | -1.69E-46 |
| MS.gene053008 | MS.gene37396 | 0.814032 | 1.89E-51 | -1.69E-46 |
| MS.gene058906 | MS.gene37396 | 0.812337 | 4.46E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene37396 | 0.810229 | 1.28E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene37396 | 0.829267 | 5.66E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37396.t1 | MTR_8g017610 | 97.561 | 82 | 2 | 0 | 4 | 85 | 15 | 96 | 3.09e-51 | 155 |
| MS.gene37396.t1 | MTR_8g017610 | 97.561 | 82 | 2 | 0 | 4 | 85 | 12 | 93 | 3.40e-51 | 155 |
| MS.gene37396.t1 | MTR_2g039890 | 41.406 | 128 | 18 | 3 | 5 | 75 | 52 | 179 | 3.77e-17 | 72.0 |
| MS.gene37396.t1 | MTR_2g039890 | 51.899 | 79 | 37 | 1 | 4 | 81 | 5 | 83 | 2.32e-16 | 69.7 |
| MS.gene37396.t1 | MTR_2g039890 | 41.406 | 128 | 18 | 3 | 5 | 75 | 99 | 226 | 4.65e-17 | 72.4 |
| MS.gene37396.t1 | MTR_2g039890 | 56.522 | 69 | 29 | 1 | 4 | 71 | 52 | 120 | 2.84e-16 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37396.t1 | AT1G72690 | 57.895 | 76 | 30 | 1 | 4 | 77 | 10 | 85 | 1.49e-25 | 90.9 |
| MS.gene37396.t1 | AT5G57000 | 54.795 | 73 | 29 | 1 | 7 | 75 | 23 | 95 | 2.74e-18 | 73.9 |
| MS.gene37396.t1 | AT5G57000 | 54.795 | 73 | 29 | 1 | 7 | 75 | 70 | 142 | 6.97e-18 | 73.9 |
| MS.gene37396.t1 | AT1G17490 | 50.725 | 69 | 27 | 1 | 10 | 78 | 15 | 76 | 3.92e-17 | 69.3 |
Find 25 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAGTGTCAGATACTTTCTT+GGG | 0.308999 | 8.4:-74322856 | None:intergenic |
| CTTAGTGTCAGATACTTTCT+TGG | 0.343708 | 8.4:-74322857 | None:intergenic |
| TTAGCCCTCTCAAGAATGTT+TGG | 0.369322 | 8.4:-74322664 | None:intergenic |
| AACAGCTATGATCACTTTGA+TGG | 0.413880 | 8.4:-74322886 | None:intergenic |
| TTCCATGAGTTTCTTTGTCA+TGG | 0.419220 | 8.4:-74322729 | None:intergenic |
| AGATACTTTCTTGGGGAGAA+TGG | 0.422648 | 8.4:-74322848 | None:intergenic |
| AAGCTCCCGGTGTGTTTGAA+AGG | 0.468558 | 8.4:+74322794 | MS.gene37396:CDS |
| AGCTCCCGGTGTGTTTGAAA+GGG | 0.525240 | 8.4:+74322795 | MS.gene37396:CDS |
| CGCCATGACAAAGAAACTCA+TGG | 0.536708 | 8.4:+74322727 | MS.gene37396:CDS |
| CAAGAAAGTATCTGACACTA+AGG | 0.540357 | 8.4:+74322858 | MS.gene37396:CDS |
| CATTCTTGAGAGGGCTAAGG+AGG | 0.553782 | 8.4:+74322669 | MS.gene37396:CDS |
| AAACATTCTTGAGAGGGCTA+AGG | 0.562461 | 8.4:+74322666 | MS.gene37396:CDS |
| CTTCACCCTTTCAAACACAC+CGG | 0.571153 | 8.4:-74322800 | None:intergenic |
| AGCTCCAAACATTCTTGAGA+GGG | 0.578483 | 8.4:+74322660 | MS.gene37396:CDS |
| GTTGAAGAAGTCAAAGCTCC+CGG | 0.580117 | 8.4:+74322781 | MS.gene37396:CDS |
| TGAGTTTCTTTGTCATGGCG+AGG | 0.584602 | 8.4:-74322724 | None:intergenic |
| CGGTGTGTTTGAAAGGGTGA+AGG | 0.593554 | 8.4:+74322801 | MS.gene37396:CDS |
| TTCACCCTTTCAAACACACC+GGG | 0.602200 | 8.4:-74322799 | None:intergenic |
| AAGCTCCAAACATTCTTGAG+AGG | 0.602370 | 8.4:+74322659 | MS.gene37396:CDS |
| TAGTGTCAGATACTTTCTTG+GGG | 0.605693 | 8.4:-74322855 | None:intergenic |
| GAGGGCTAAGGAGGAGACTG+AGG | 0.618637 | 8.4:+74322678 | MS.gene37396:CDS |
| AGAGCTTGAAGCTGTAGTTG+AGG | 0.634155 | 8.4:+74322825 | MS.gene37396:CDS |
| ATGACAAAGAAACTCATGGA+AGG | 0.643744 | 8.4:+74322731 | MS.gene37396:CDS |
| AAGGAATGATGACATTGATG+AGG | 0.657096 | 8.4:+74322750 | MS.gene37396:CDS |
| AGGAATGATGACATTGATGA+GGG | 0.670735 | 8.4:+74322751 | MS.gene37396:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAACTATTTTATAAATGATT+TGG | + | chr8.4:74322573-74322592 | MS.gene37396:intron | 10.0% |
| !! | AATGAAACAGAATATACAAA+AGG | - | chr8.4:74322621-74322640 | None:intergenic | 20.0% |
| !! | ATGAAACAGAATATACAAAA+GGG | - | chr8.4:74322620-74322639 | None:intergenic | 20.0% |
| !!! | GATAAGTAATTATTGGTTTT+AGG | + | chr8.4:74322499-74322518 | MS.gene37396:intron | 20.0% |
| ! | TGAAACAGAATATACAAAAG+GGG | - | chr8.4:74322619-74322638 | None:intergenic | 25.0% |
| !!! | TATTGGTTTTAGGTATATGT+GGG | + | chr8.4:74322509-74322528 | MS.gene37396:intron | 25.0% |
| !!! | TTATTGGTTTTAGGTATATG+TGG | + | chr8.4:74322508-74322527 | MS.gene37396:intron | 25.0% |
| TTAGTGTCAGATACTTTCTT+GGG | - | chr8.4:74322859-74322878 | None:intergenic | 30.0% | |
| AAGGAATGATGACATTGATG+AGG | + | chr8.4:74322750-74322769 | MS.gene37396:CDS | 35.0% | |
| AGGAATGATGACATTGATGA+GGG | + | chr8.4:74322751-74322770 | MS.gene37396:CDS | 35.0% | |
| ATGACAAAGAAACTCATGGA+AGG | + | chr8.4:74322731-74322750 | MS.gene37396:CDS | 35.0% | |
| CTTAGTGTCAGATACTTTCT+TGG | - | chr8.4:74322860-74322879 | None:intergenic | 35.0% | |
| TAGTGTCAGATACTTTCTTG+GGG | - | chr8.4:74322858-74322877 | None:intergenic | 35.0% | |
| TTCCATGAGTTTCTTTGTCA+TGG | - | chr8.4:74322732-74322751 | None:intergenic | 35.0% | |
| !! | CAAGAAAGTATCTGACACTA+AGG | + | chr8.4:74322858-74322877 | MS.gene37396:CDS | 35.0% |
| AAACATTCTTGAGAGGGCTA+AGG | + | chr8.4:74322666-74322685 | MS.gene37396:CDS | 40.0% | |
| AAGCTCCAAACATTCTTGAG+AGG | + | chr8.4:74322659-74322678 | MS.gene37396:CDS | 40.0% | |
| AGATACTTTCTTGGGGAGAA+TGG | - | chr8.4:74322851-74322870 | None:intergenic | 40.0% | |
| AGCTCCAAACATTCTTGAGA+GGG | + | chr8.4:74322660-74322679 | MS.gene37396:CDS | 40.0% | |
| TTAGCCCTCTCAAGAATGTT+TGG | - | chr8.4:74322667-74322686 | None:intergenic | 40.0% | |
| AGAGCTTGAAGCTGTAGTTG+AGG | + | chr8.4:74322825-74322844 | MS.gene37396:CDS | 45.0% | |
| CGCCATGACAAAGAAACTCA+TGG | + | chr8.4:74322727-74322746 | MS.gene37396:CDS | 45.0% | |
| CTTCACCCTTTCAAACACAC+CGG | - | chr8.4:74322803-74322822 | None:intergenic | 45.0% | |
| GTTGAAGAAGTCAAAGCTCC+CGG | + | chr8.4:74322781-74322800 | MS.gene37396:CDS | 45.0% | |
| TGAGTTTCTTTGTCATGGCG+AGG | - | chr8.4:74322727-74322746 | None:intergenic | 45.0% | |
| TTCACCCTTTCAAACACACC+GGG | - | chr8.4:74322802-74322821 | None:intergenic | 45.0% | |
| AAGCTCCCGGTGTGTTTGAA+AGG | + | chr8.4:74322794-74322813 | MS.gene37396:CDS | 50.0% | |
| AGCTCCCGGTGTGTTTGAAA+GGG | + | chr8.4:74322795-74322814 | MS.gene37396:CDS | 50.0% | |
| CATTCTTGAGAGGGCTAAGG+AGG | + | chr8.4:74322669-74322688 | MS.gene37396:CDS | 50.0% | |
| CGGTGTGTTTGAAAGGGTGA+AGG | + | chr8.4:74322801-74322820 | MS.gene37396:CDS | 50.0% | |
| GAGGGCTAAGGAGGAGACTG+AGG | + | chr8.4:74322678-74322697 | MS.gene37396:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 74322497 | 74322897 | 74322497 | ID=MS.gene37396 |
| chr8.4 | mRNA | 74322497 | 74322897 | 74322497 | ID=MS.gene37396.t1;Parent=MS.gene37396 |
| chr8.4 | exon | 74322497 | 74322503 | 74322497 | ID=MS.gene37396.t1.exon1;Parent=MS.gene37396.t1 |
| chr8.4 | CDS | 74322497 | 74322503 | 74322497 | ID=cds.MS.gene37396.t1;Parent=MS.gene37396.t1 |
| chr8.4 | exon | 74322647 | 74322897 | 74322647 | ID=MS.gene37396.t1.exon2;Parent=MS.gene37396.t1 |
| chr8.4 | CDS | 74322647 | 74322897 | 74322647 | ID=cds.MS.gene37396.t1;Parent=MS.gene37396.t1 |
| Gene Sequence |
| Protein sequence |
