Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059151.t1 | XP_003588519.1 | 99.6 | 249 | 1 | 0 | 1 | 249 | 85 | 333 | 1.00E-151 | 545.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059151.t1 | Q84JL3 | 92.4 | 249 | 17 | 1 | 1 | 247 | 77 | 325 | 9.1e-141 | 500.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059151.t1 | A8W460 | 99.6 | 249 | 1 | 0 | 1 | 249 | 85 | 333 | 7.4e-152 | 545.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene059151 | 0.810677 | 1.02E-50 | -1.69E-46 |
| MS.gene050303 | MS.gene059151 | 0.803198 | 3.91E-49 | -1.69E-46 |
| MS.gene050689 | MS.gene059151 | 0.810157 | 1.33E-50 | -1.69E-46 |
| MS.gene050815 | MS.gene059151 | 0.814772 | 1.30E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene059151 | 0.813978 | 1.95E-51 | -1.69E-46 |
| MS.gene05134 | MS.gene059151 | 0.81338 | 2.64E-51 | -1.69E-46 |
| MS.gene052012 | MS.gene059151 | 0.801433 | 9.03E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene059151 | 0.809112 | 2.22E-50 | -1.69E-46 |
| MS.gene052611 | MS.gene059151 | 0.800118 | 1.67E-48 | -1.69E-46 |
| MS.gene052863 | MS.gene059151 | 0.816268 | 6.04E-52 | -1.69E-46 |
| MS.gene053008 | MS.gene059151 | 0.867401 | 1.41E-65 | -1.69E-46 |
| MS.gene05517 | MS.gene059151 | 0.812133 | 4.94E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene059151 | 0.80823 | 3.43E-50 | -1.69E-46 |
| MS.gene056215 | MS.gene059151 | 0.80387 | 2.84E-49 | -1.69E-46 |
| MS.gene057140 | MS.gene059151 | 0.807172 | 5.75E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene059151 | 0.81793 | 2.55E-52 | -1.69E-46 |
| MS.gene058783 | MS.gene059151 | 0.806638 | 7.46E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene059842 | 0.814527 | 1.47E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene060322 | 0.802585 | 5.23E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene060429 | 0.802229 | 6.19E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene060586 | 0.809003 | 2.35E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene060803 | 0.800552 | 1.37E-48 | -1.69E-46 |
| MS.gene059151 | MS.gene061866 | 0.812056 | 5.14E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene061869 | 0.81687 | 4.43E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene061874 | 0.808182 | 3.51E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene061877 | 0.877466 | 6.14E-69 | -1.69E-46 |
| MS.gene059151 | MS.gene063025 | 0.80382 | 2.90E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene064070 | 0.82518 | 5.40E-54 | -1.69E-46 |
| MS.gene059151 | MS.gene064071 | 0.801979 | 6.97E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene064072 | 0.801259 | 9.80E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene064353 | 0.820579 | 6.37E-53 | -1.69E-46 |
| MS.gene059151 | MS.gene066105 | 0.828652 | 7.98E-55 | -1.69E-46 |
| MS.gene059151 | MS.gene068245 | 0.803854 | 2.86E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene068777 | 0.827306 | 1.68E-54 | -1.69E-46 |
| MS.gene059151 | MS.gene070242 | 0.818179 | 2.24E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene071480 | 0.851496 | 8.55E-61 | -1.69E-46 |
| MS.gene059151 | MS.gene071481 | 0.833116 | 6.42E-56 | -1.69E-46 |
| MS.gene059151 | MS.gene073954 | 0.802557 | 5.30E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene07420 | 0.813176 | 2.92E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene074603 | 0.80595 | 1.04E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene074606 | 0.845421 | 4.11E-59 | -1.69E-46 |
| MS.gene059151 | MS.gene074616 | 0.811344 | 7.34E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene074617 | 0.812236 | 4.69E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene08428 | 0.838346 | 3.04E-57 | -1.69E-46 |
| MS.gene059151 | MS.gene08813 | 0.825762 | 3.93E-54 | -1.69E-46 |
| MS.gene059151 | MS.gene09277 | 0.822298 | 2.55E-53 | -1.69E-46 |
| MS.gene059151 | MS.gene09873 | 0.825011 | 5.91E-54 | -1.69E-46 |
| MS.gene059151 | MS.gene21928 | 0.804901 | 1.73E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene22218 | 0.805828 | 1.11E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene22270 | 0.815342 | 9.71E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene22740 | 0.824559 | 7.56E-54 | -1.69E-46 |
| MS.gene059151 | MS.gene23006 | 0.83351 | 5.12E-56 | -1.69E-46 |
| MS.gene059151 | MS.gene23889 | 0.830002 | 3.75E-55 | -1.69E-46 |
| MS.gene059151 | MS.gene24623 | 0.804273 | 2.34E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene24626 | 0.8131 | 3.04E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene24628 | 0.807536 | 4.82E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene27947 | 0.808967 | 2.39E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene28658 | 0.816289 | 5.97E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene28944 | 0.851278 | 9.85E-61 | -1.69E-46 |
| MS.gene059151 | MS.gene30335 | 0.807738 | 4.37E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene30920 | 0.811549 | 6.62E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene35048 | 0.81438 | 1.59E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene35135 | 0.829032 | 6.46E-55 | -1.69E-46 |
| MS.gene059151 | MS.gene35214 | 0.821864 | 3.22E-53 | -1.69E-46 |
| MS.gene059151 | MS.gene35611 | 0.829568 | 4.79E-55 | -1.69E-46 |
| MS.gene059151 | MS.gene37396 | 0.810229 | 1.28E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene39408 | 0.800667 | 1.29E-48 | -1.69E-46 |
| MS.gene059151 | MS.gene39572 | 0.839369 | 1.65E-57 | -1.69E-46 |
| MS.gene059151 | MS.gene39882 | 0.831456 | 1.65E-55 | -1.69E-46 |
| MS.gene059151 | MS.gene40595 | 0.814399 | 1.57E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene40971 | 0.824453 | 8.01E-54 | -1.69E-46 |
| MS.gene059151 | MS.gene43992 | 0.827796 | 1.28E-54 | -1.69E-46 |
| MS.gene059151 | MS.gene47338 | 0.811286 | 7.55E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene47339 | 0.812096 | 5.03E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene47340 | 0.811444 | 6.98E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene47531 | 0.843427 | 1.41E-58 | -1.69E-46 |
| MS.gene059151 | MS.gene49283 | 0.813864 | 2.06E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene49320 | 0.817788 | 2.75E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene49793 | 0.808896 | 2.47E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene50708 | 0.801326 | 9.49E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene51788 | 0.802425 | 5.64E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene52357 | 0.823653 | 1.23E-53 | -1.69E-46 |
| MS.gene059151 | MS.gene53579 | 0.825234 | 5.24E-54 | -1.69E-46 |
| MS.gene059151 | MS.gene54556 | 0.946008 | 1.04E-104 | -1.69E-46 |
| MS.gene059151 | MS.gene54919 | 0.802336 | 5.89E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene55887 | 0.805908 | 1.06E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene56067 | 0.816825 | 4.53E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene56414 | 0.816454 | 5.48E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene59769 | 0.811265 | 7.63E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene59949 | 0.800306 | 1.53E-48 | -1.69E-46 |
| MS.gene059151 | MS.gene60019 | 0.815603 | 8.50E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene62009 | 0.812045 | 5.17E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene62393 | 0.811677 | 6.21E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene64071 | 0.859662 | 3.54E-63 | -1.69E-46 |
| MS.gene059151 | MS.gene64243 | 0.80169 | 7.99E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene64650 | 0.819196 | 1.32E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene67217 | 0.800309 | 1.53E-48 | -1.69E-46 |
| MS.gene059151 | MS.gene68735 | 0.803102 | 4.09E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene69947 | 0.82781 | 1.27E-54 | -1.69E-46 |
| MS.gene059151 | MS.gene70398 | 0.807534 | 4.82E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene70460 | 0.804846 | 1.78E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene71299 | 0.815516 | 8.88E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene71705 | 0.801447 | 8.97E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene71949 | 0.812811 | 3.51E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene72203 | 0.811197 | 7.90E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene72347 | 0.817899 | 2.60E-52 | -1.69E-46 |
| MS.gene059151 | MS.gene72413 | 0.808935 | 2.42E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene73364 | 0.80959 | 1.75E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene73640 | 0.808237 | 3.42E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene75003 | 0.801762 | 7.73E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene76344 | 0.804804 | 1.81E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene78659 | 0.808997 | 2.35E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene81900 | 0.820529 | 6.54E-53 | -1.69E-46 |
| MS.gene059151 | MS.gene82879 | 0.809204 | 2.12E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene87407 | 0.834718 | 2.55E-56 | -1.69E-46 |
| MS.gene059151 | MS.gene87954 | 0.814587 | 1.43E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene88145 | 0.811442 | 6.99E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene88243 | 0.800037 | 1.74E-48 | -1.69E-46 |
| MS.gene059151 | MS.gene88371 | 0.805732 | 1.16E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene88680 | 0.810193 | 1.30E-50 | -1.69E-46 |
| MS.gene059151 | MS.gene90861 | 0.8237 | 1.20E-53 | -1.69E-46 |
| MS.gene059151 | MS.gene90981 | 0.812842 | 3.46E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene92188 | 0.814725 | 1.33E-51 | -1.69E-46 |
| MS.gene059151 | MS.gene95625 | 0.805846 | 1.10E-49 | -1.69E-46 |
| MS.gene059151 | MS.gene95675 | 0.800858 | 1.18E-48 | -1.69E-46 |
| MS.gene059151 | MS.gene99657 | 0.816919 | 4.31E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059151.t1 | MTR_1g008120 | 99.598 | 249 | 1 | 0 | 1 | 249 | 85 | 333 | 0.0 | 527 |
| MS.gene059151.t1 | MTR_3g091510 | 95.582 | 249 | 10 | 1 | 1 | 249 | 77 | 324 | 0.0 | 505 |
| MS.gene059151.t1 | MTR_8g027520 | 89.960 | 249 | 25 | 0 | 1 | 249 | 70 | 318 | 8.13e-177 | 489 |
| MS.gene059151.t1 | MTR_8g027520 | 89.960 | 249 | 25 | 0 | 1 | 249 | 61 | 309 | 1.02e-176 | 488 |
| MS.gene059151.t1 | MTR_7g117415 | 83.534 | 249 | 38 | 1 | 1 | 249 | 78 | 323 | 1.07e-161 | 451 |
| MS.gene059151.t1 | MTR_3g017590 | 83.830 | 235 | 38 | 0 | 1 | 235 | 70 | 304 | 5.65e-153 | 428 |
| MS.gene059151.t1 | MTR_8g038370 | 78.298 | 235 | 51 | 0 | 1 | 235 | 129 | 363 | 2.67e-145 | 410 |
| MS.gene059151.t1 | MTR_7g093600 | 78.298 | 235 | 51 | 0 | 1 | 235 | 76 | 310 | 7.65e-142 | 400 |
| MS.gene059151.t1 | MTR_1g054335 | 77.447 | 235 | 53 | 0 | 1 | 235 | 75 | 309 | 4.90e-140 | 395 |
| MS.gene059151.t1 | MTR_5g076660 | 32.667 | 150 | 95 | 3 | 1 | 150 | 50 | 193 | 8.38e-17 | 79.0 |
| MS.gene059151.t1 | MTR_7g024290 | 26.042 | 192 | 132 | 4 | 1 | 192 | 28 | 209 | 1.19e-15 | 75.5 |
| MS.gene059151.t1 | MTR_5g067400 | 33.607 | 122 | 79 | 2 | 1 | 121 | 28 | 148 | 5.14e-15 | 73.6 |
| MS.gene059151.t1 | MTR_4g094468 | 27.000 | 200 | 125 | 7 | 1 | 195 | 114 | 297 | 6.88e-14 | 70.5 |
| MS.gene059151.t1 | MTR_2g038430 | 33.036 | 112 | 70 | 2 | 1 | 109 | 42 | 151 | 1.81e-13 | 69.3 |
| MS.gene059151.t1 | MTR_5g076370 | 25.551 | 227 | 153 | 6 | 1 | 227 | 51 | 261 | 2.14e-13 | 68.9 |
| MS.gene059151.t1 | MTR_7g094390 | 36.364 | 99 | 61 | 1 | 1 | 99 | 189 | 285 | 4.43e-13 | 68.6 |
| MS.gene059151.t1 | MTR_5g061290 | 32.479 | 117 | 73 | 4 | 1 | 116 | 35 | 146 | 8.97e-13 | 67.0 |
| MS.gene059151.t1 | MTR_5g076540 | 32.903 | 155 | 88 | 5 | 1 | 150 | 28 | 171 | 1.27e-12 | 65.9 |
| MS.gene059151.t1 | MTR_5g076720 | 33.036 | 112 | 70 | 2 | 1 | 109 | 99 | 208 | 2.89e-12 | 65.9 |
| MS.gene059151.t1 | MTR_5g076505 | 29.677 | 155 | 93 | 5 | 1 | 150 | 24 | 167 | 3.52e-12 | 65.5 |
| MS.gene059151.t1 | MTR_5g076520 | 30.667 | 150 | 98 | 3 | 1 | 150 | 28 | 171 | 3.46e-11 | 62.4 |
| MS.gene059151.t1 | MTR_1g046650 | 34.343 | 99 | 62 | 2 | 1 | 99 | 28 | 123 | 4.05e-11 | 62.4 |
| MS.gene059151.t1 | MTR_2g038420 | 29.310 | 116 | 79 | 2 | 1 | 116 | 42 | 154 | 5.76e-11 | 62.0 |
| MS.gene059151.t1 | MTR_1g046650 | 34.343 | 99 | 62 | 2 | 1 | 99 | 59 | 154 | 6.17e-11 | 62.0 |
| MS.gene059151.t1 | MTR_1g046650 | 34.343 | 99 | 62 | 2 | 1 | 99 | 28 | 123 | 6.20e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059151.t1 | AT3G61790 | 92.369 | 249 | 17 | 1 | 1 | 247 | 77 | 325 | 1.17e-175 | 486 |
| MS.gene059151.t1 | AT4G27880 | 89.157 | 249 | 27 | 0 | 1 | 249 | 78 | 326 | 2.08e-173 | 480 |
| MS.gene059151.t1 | AT5G53360 | 89.908 | 218 | 22 | 0 | 32 | 249 | 15 | 232 | 1.60e-152 | 424 |
| MS.gene059151.t1 | AT5G53360 | 89.908 | 218 | 22 | 0 | 32 | 249 | 15 | 232 | 1.60e-152 | 424 |
| MS.gene059151.t1 | AT3G58040 | 82.553 | 235 | 41 | 0 | 1 | 235 | 74 | 308 | 4.25e-148 | 416 |
| MS.gene059151.t1 | AT3G58040 | 82.553 | 235 | 41 | 0 | 1 | 235 | 74 | 308 | 4.25e-148 | 416 |
| MS.gene059151.t1 | AT2G41980 | 80.426 | 235 | 46 | 0 | 1 | 235 | 71 | 305 | 5.82e-145 | 407 |
| MS.gene059151.t1 | AT5G53360 | 89.167 | 120 | 13 | 0 | 130 | 249 | 60 | 179 | 2.32e-79 | 237 |
| MS.gene059151.t1 | AT3G58040 | 76.596 | 141 | 30 | 1 | 1 | 138 | 74 | 214 | 8.60e-75 | 226 |
| MS.gene059151.t1 | AT3G13672 | 58.333 | 168 | 67 | 1 | 71 | 235 | 45 | 212 | 2.05e-72 | 220 |
| MS.gene059151.t1 | AT3G13672 | 50.769 | 195 | 66 | 2 | 71 | 235 | 45 | 239 | 5.91e-67 | 207 |
| MS.gene059151.t1 | AT5G37870 | 40.000 | 110 | 63 | 2 | 1 | 110 | 59 | 165 | 3.25e-19 | 84.7 |
| MS.gene059151.t1 | AT5G37930 | 40.777 | 103 | 59 | 1 | 1 | 103 | 127 | 227 | 7.04e-18 | 82.0 |
| MS.gene059151.t1 | AT5G37890 | 26.638 | 229 | 153 | 6 | 1 | 227 | 65 | 280 | 2.41e-16 | 77.0 |
| MS.gene059151.t1 | AT5G62800 | 25.328 | 229 | 159 | 6 | 1 | 227 | 57 | 275 | 1.48e-15 | 75.5 |
| MS.gene059151.t1 | AT5G62800 | 25.532 | 188 | 132 | 4 | 1 | 186 | 57 | 238 | 3.54e-15 | 73.9 |
| MS.gene059151.t1 | AT1G66660 | 40.426 | 94 | 54 | 1 | 1 | 94 | 120 | 211 | 1.70e-14 | 72.4 |
| MS.gene059151.t1 | AT1G66650 | 37.255 | 102 | 61 | 2 | 1 | 101 | 100 | 199 | 2.83e-14 | 71.6 |
| MS.gene059151.t1 | AT5G37910 | 34.951 | 103 | 65 | 1 | 1 | 103 | 52 | 152 | 7.65e-13 | 67.0 |
| MS.gene059151.t1 | AT1G66620 | 35.354 | 99 | 62 | 1 | 1 | 99 | 58 | 154 | 1.13e-12 | 67.0 |
| MS.gene059151.t1 | AT1G66630 | 37.500 | 104 | 61 | 2 | 1 | 102 | 63 | 164 | 4.57e-12 | 65.1 |
Find 67 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTAGTATGGAAAGATTTC+TGG | 0.159871 | 1.2:+11585234 | None:intergenic |
| TGATAGGTATTTCATTGTTT+TGG | 0.211856 | 1.2:-11584666 | MS.gene059151:intron |
| CCTCATAATTCAACGAAATA+TGG | 0.309243 | 1.2:-11584436 | MS.gene059151:CDS |
| TGCTTCAGCTTGCTGTAGTA+TGG | 0.315397 | 1.2:+11585222 | None:intergenic |
| TAAGAGTCACAGGAAGAATA+TGG | 0.339068 | 1.2:-11584374 | MS.gene059151:CDS |
| CGAAGGAATGCCATGTAAAC+TGG | 0.349784 | 1.2:+11584597 | None:intergenic |
| CATGGCATTCCTTCGTTTCA+TGG | 0.350645 | 1.2:-11584589 | MS.gene059151:CDS |
| AGCCTCGAGGTTGGGGCAAA+TGG | 0.361379 | 1.2:-11584534 | MS.gene059151:CDS |
| CAAATGGAAGAAAACTCATT+TGG | 0.371206 | 1.2:-11584518 | MS.gene059151:CDS |
| ACACATCAGCTGCAAAGATT+TGG | 0.392931 | 1.2:+11584315 | None:intergenic |
| ATACCTACAGCCTCGAGGTT+GGG | 0.402306 | 1.2:-11584542 | MS.gene059151:CDS |
| CCATATTTCGTTGAATTATG+AGG | 0.425488 | 1.2:+11584436 | None:intergenic |
| TAGGTGTCTGGCACTGGAAA+AGG | 0.426106 | 1.2:-11585304 | MS.gene059151:CDS |
| CCATGCAAATACTACTCTCT+TGG | 0.426909 | 1.2:-11585261 | MS.gene059151:CDS |
| AGATATTAGGTGTCTGGCAC+TGG | 0.427576 | 1.2:-11585310 | MS.gene059151:CDS |
| GGTATACAAACCCCACCATC+TGG | 0.433787 | 1.2:+11584336 | None:intergenic |
| GACAAGAGCTTGGAGATATT+AGG | 0.446367 | 1.2:-11585323 | MS.gene059151:CDS |
| TTTCCTCGTTGCACATTTGA+GGG | 0.453343 | 1.2:-11585124 | MS.gene059151:CDS |
| ATTTCCTCGTTGCACATTTG+AGG | 0.454644 | 1.2:-11585125 | MS.gene059151:CDS |
| CAAAGTGAAGGCAGAAGTAT+TGG | 0.460533 | 1.2:+11584643 | None:intergenic |
| ACATTTGAGGGACGATCATA+AGG | 0.461663 | 1.2:-11585112 | MS.gene059151:CDS |
| ATCTCCAAGCTCTTGTCTAC+AGG | 0.469456 | 1.2:+11585329 | None:intergenic |
| AAATGGAAGAAAACTCATTT+GGG | 0.470132 | 1.2:-11584517 | MS.gene059151:CDS |
| CACTTTGAAGCTTTCCAGCT+AGG | 0.473624 | 1.2:-11584627 | MS.gene059151:CDS |
| AAACTGGAGCCATGCCTAGC+TGG | 0.474045 | 1.2:+11584613 | None:intergenic |
| GCTTGGAGATATTAGGTGTC+TGG | 0.474212 | 1.2:-11585316 | MS.gene059151:CDS |
| ATGGCATTCCTTCGTTTCAT+GGG | 0.491316 | 1.2:-11584588 | MS.gene059151:CDS |
| TACCTACAGCCTCGAGGTTG+GGG | 0.519942 | 1.2:-11584541 | MS.gene059151:CDS |
| CAGTTCCTTCCTATCACCAC+CGG | 0.520559 | 1.2:+11584401 | None:intergenic |
| CCATACACTTGTCCTTATGC+TGG | 0.527298 | 1.2:-11585177 | MS.gene059151:CDS |
| GAACTGAAATTAAGAGTCAC+AGG | 0.528820 | 1.2:-11584384 | MS.gene059151:CDS |
| GAGCACTCTGATCCAGCATA+AGG | 0.535134 | 1.2:+11585165 | None:intergenic |
| AGGCATGGCTCCAGTTTACA+TGG | 0.535252 | 1.2:-11584607 | MS.gene059151:CDS |
| CCAGCATAAGGACAAGTGTA+TGG | 0.536800 | 1.2:+11585177 | None:intergenic |
| TGAAGCTTTCCAGCTAGGCA+TGG | 0.545484 | 1.2:-11584622 | MS.gene059151:CDS |
| TTTCTCCGGTGGTGATAGGA+AGG | 0.551436 | 1.2:-11584406 | MS.gene059151:CDS |
| AACAACAGAATCCAGATGGT+GGG | 0.552181 | 1.2:-11584347 | MS.gene059151:CDS |
| TATACCTACAGCCTCGAGGT+TGG | 0.558753 | 1.2:-11584543 | MS.gene059151:CDS |
| GGATCAGAGTGCTCTGCTGT+TGG | 0.562708 | 1.2:-11585156 | MS.gene059151:CDS |
| CCGACCTGTAGACAAGAGCT+TGG | 0.568050 | 1.2:-11585333 | MS.gene059151:CDS |
| GTGAAGTAGAGAATGCTACT+TGG | 0.568738 | 1.2:-11585035 | MS.gene059151:CDS |
| TGGGAGACGAGAACGAGGCT+CGG | 0.575368 | 1.2:-11584569 | MS.gene059151:CDS |
| TTCCATTTGCCCCAACCTCG+AGG | 0.594074 | 1.2:+11584532 | None:intergenic |
| CCAAGAGAGTAGTATTTGCA+TGG | 0.602573 | 1.2:+11585261 | None:intergenic |
| TGGAAGAAAACTCATTTGGG+AGG | 0.602668 | 1.2:-11584514 | MS.gene059151:CDS |
| GAACAACAGAATCCAGATGG+TGG | 0.613047 | 1.2:-11584348 | MS.gene059151:CDS |
| GCTGGAAAGCTTCAAAGTGA+AGG | 0.613710 | 1.2:+11584631 | None:intergenic |
| ACAACAGAATCCAGATGGTG+GGG | 0.614888 | 1.2:-11584346 | MS.gene059151:CDS |
| GGAAGAAAACTCATTTGGGA+GGG | 0.623722 | 1.2:-11584513 | MS.gene059151:CDS |
| CATAAGGTGGACATGCACAC+AGG | 0.632218 | 1.2:-11585096 | MS.gene059151:CDS |
| TTTCATGGGAGACGAGAACG+AGG | 0.636980 | 1.2:-11584574 | MS.gene059151:CDS |
| TGTGTTCCACATGTAAAACA+AGG | 0.639674 | 1.2:-11585371 | MS.gene059151:CDS |
| TGCCCCAACCTCGAGGCTGT+AGG | 0.640496 | 1.2:+11584539 | None:intergenic |
| AAAGAACAACAGAATCCAGA+TGG | 0.642877 | 1.2:-11584351 | MS.gene059151:CDS |
| GATTTGACTTCACGTAACGA+TGG | 0.651834 | 1.2:+11585061 | None:intergenic |
| AAAGTTCGAGATAGTCATGA+CGG | 0.664593 | 1.2:-11584459 | MS.gene059151:CDS |
| CCAAGCTCTTGTCTACAGGT+CGG | 0.665523 | 1.2:+11585333 | None:intergenic |
| GCTTTCTTGCAGTGCCACAA+CGG | 0.673342 | 1.2:-11585402 | None:intergenic |
| TCTCGTCTCCCATGAAACGA+AGG | 0.676802 | 1.2:+11584580 | None:intergenic |
| TGGCTATCGCGGATACTCCG+TGG | 0.686071 | 1.2:+11584486 | None:intergenic |
| TTTGAGGGACGATCATAAGG+TGG | 0.697457 | 1.2:-11585109 | MS.gene059151:CDS |
| GTGTTCCACATGTAAAACAA+GGG | 0.699010 | 1.2:-11585370 | MS.gene059151:CDS |
| GAACTATACCTACAGCCTCG+AGG | 0.705784 | 1.2:-11584547 | MS.gene059151:CDS |
| CGTCCCTCAAATGTGCAACG+AGG | 0.714067 | 1.2:+11585121 | None:intergenic |
| GTAGCATTCTCTACTTCACG+CGG | 0.721685 | 1.2:+11585039 | None:intergenic |
| AACACAATGTGTGACCGTTG+TGG | 0.724979 | 1.2:+11585388 | None:intergenic |
| TCATTTGGGAGGGTACACCA+CGG | 0.762771 | 1.2:-11584503 | MS.gene059151:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAAAATCTATATTTGTAA+CGG | + | chr1.2:11584771-11584790 | None:intergenic | 15.0% |
| !! | AATACCTATCAAAGAAAAAA+TGG | + | chr1.2:11585035-11585054 | None:intergenic | 20.0% |
| ! | AAATGGAAGAAAACTCATTT+GGG | - | chr1.2:11585193-11585212 | MS.gene059151:CDS | 25.0% |
| ! | ATCAAAGAAAAAATGGAAGT+TGG | + | chr1.2:11585028-11585047 | None:intergenic | 25.0% |
| !! | TAATGTGCAACTTTGTATTT+TGG | - | chr1.2:11584833-11584852 | MS.gene059151:intron | 25.0% |
| !! | TGATAGGTATTTCATTGTTT+TGG | - | chr1.2:11585044-11585063 | MS.gene059151:CDS | 25.0% |
| !!! | ACTTCCATTTTTTCTTTGAT+AGG | - | chr1.2:11585028-11585047 | MS.gene059151:CDS | 25.0% |
| CAAATGGAAGAAAACTCATT+TGG | - | chr1.2:11585192-11585211 | MS.gene059151:CDS | 30.0% | |
| CCATATTTCGTTGAATTATG+AGG | + | chr1.2:11585277-11585296 | None:intergenic | 30.0% | |
| CCTCATAATTCAACGAAATA+TGG | - | chr1.2:11585274-11585293 | MS.gene059151:CDS | 30.0% | |
| AAAGAACAACAGAATCCAGA+TGG | - | chr1.2:11585359-11585378 | MS.gene059151:CDS | 35.0% | |
| AAAGTTCGAGATAGTCATGA+CGG | - | chr1.2:11585251-11585270 | MS.gene059151:CDS | 35.0% | |
| ACTGTCAGATCAAGATCAAA+TGG | + | chr1.2:11584887-11584906 | None:intergenic | 35.0% | |
| CTGTAGTATGGAAAGATTTC+TGG | + | chr1.2:11584479-11584498 | None:intergenic | 35.0% | |
| GAACTGAAATTAAGAGTCAC+AGG | - | chr1.2:11585326-11585345 | MS.gene059151:CDS | 35.0% | |
| GTGTTCCACATGTAAAACAA+GGG | - | chr1.2:11584340-11584359 | MS.gene059151:CDS | 35.0% | |
| TAAGAGTCACAGGAAGAATA+TGG | - | chr1.2:11585336-11585355 | MS.gene059151:CDS | 35.0% | |
| TGTGTTCCACATGTAAAACA+AGG | - | chr1.2:11584339-11584358 | MS.gene059151:CDS | 35.0% | |
| ! | CATGACTATCTCGAACTTTT+CGG | + | chr1.2:11585250-11585269 | None:intergenic | 35.0% |
| !! | AATATGGCCCTATTTTTCTC+CGG | - | chr1.2:11585290-11585309 | MS.gene059151:CDS | 35.0% |
| AACAACAGAATCCAGATGGT+GGG | - | chr1.2:11585363-11585382 | MS.gene059151:CDS | 40.0% | |
| AACGGCACATATTTGACAGA+GGG | + | chr1.2:11584753-11584772 | None:intergenic | 40.0% | |
| AATGGCTCGTGTTTCAACTT+TGG | + | chr1.2:11584869-11584888 | None:intergenic | 40.0% | |
| ACATTTGAGGGACGATCATA+AGG | - | chr1.2:11584598-11584617 | MS.gene059151:CDS | 40.0% | |
| ATCACCACCGGAGAAAAATA+GGG | + | chr1.2:11585300-11585319 | None:intergenic | 40.0% | |
| ATCACCAGATCAAGATTGGA+CGG | - | chr1.2:11584809-11584828 | MS.gene059151:intron | 40.0% | |
| ATGGCATTCCTTCGTTTCAT+GGG | - | chr1.2:11585122-11585141 | MS.gene059151:CDS | 40.0% | |
| ATTTCCTCGTTGCACATTTG+AGG | - | chr1.2:11584585-11584604 | MS.gene059151:CDS | 40.0% | |
| CACAATCACCAGATCAAGAT+TGG | - | chr1.2:11584805-11584824 | MS.gene059151:intron | 40.0% | |
| CCAAGAGAGTAGTATTTGCA+TGG | + | chr1.2:11584452-11584471 | None:intergenic | 40.0% | |
| CCATGCAAATACTACTCTCT+TGG | - | chr1.2:11584449-11584468 | MS.gene059151:CDS | 40.0% | |
| GACAAGAGCTTGGAGATATT+AGG | - | chr1.2:11584387-11584406 | MS.gene059151:CDS | 40.0% | |
| GGAAGAAAACTCATTTGGGA+GGG | - | chr1.2:11585197-11585216 | MS.gene059151:CDS | 40.0% | |
| GTGAAGTAGAGAATGCTACT+TGG | - | chr1.2:11584675-11584694 | MS.gene059151:intron | 40.0% | |
| TAACGGCACATATTTGACAG+AGG | + | chr1.2:11584754-11584773 | None:intergenic | 40.0% | |
| TATCACCACCGGAGAAAAAT+AGG | + | chr1.2:11585301-11585320 | None:intergenic | 40.0% | |
| TGGAAGAAAACTCATTTGGG+AGG | - | chr1.2:11585196-11585215 | MS.gene059151:CDS | 40.0% | |
| TTTCCTCGTTGCACATTTGA+GGG | - | chr1.2:11584586-11584605 | MS.gene059151:CDS | 40.0% | |
| ! | CAAAGTGAAGGCAGAAGTAT+TGG | + | chr1.2:11585070-11585089 | None:intergenic | 40.0% |
| ! | GATTTGACTTCACGTAACGA+TGG | + | chr1.2:11584652-11584671 | None:intergenic | 40.0% |
| ! | TATTTTTCTCCGGTGGTGAT+AGG | - | chr1.2:11585300-11585319 | MS.gene059151:CDS | 40.0% |
| AACACAATGTGTGACCGTTG+TGG | + | chr1.2:11584325-11584344 | None:intergenic | 45.0% | |
| ACAACAGAATCCAGATGGTG+GGG | - | chr1.2:11585364-11585383 | MS.gene059151:CDS | 45.0% | |
| ACTCGTCAGCTCGTTATTCA+CGG | + | chr1.2:11584923-11584942 | None:intergenic | 45.0% | |
| AGATATTAGGTGTCTGGCAC+TGG | - | chr1.2:11584400-11584419 | MS.gene059151:CDS | 45.0% | |
| ATCTCCAAGCTCTTGTCTAC+AGG | + | chr1.2:11584384-11584403 | None:intergenic | 45.0% | |
| CATGGCATTCCTTCGTTTCA+TGG | - | chr1.2:11585121-11585140 | MS.gene059151:CDS | 45.0% | |
| CCAGCATAAGGACAAGTGTA+TGG | + | chr1.2:11584536-11584555 | None:intergenic | 45.0% | |
| CCATACACTTGTCCTTATGC+TGG | - | chr1.2:11584533-11584552 | MS.gene059151:CDS | 45.0% | |
| CGAAGGAATGCCATGTAAAC+TGG | + | chr1.2:11585116-11585135 | None:intergenic | 45.0% | |
| GAACAACAGAATCCAGATGG+TGG | - | chr1.2:11585362-11585381 | MS.gene059151:CDS | 45.0% | |
| GCTGGAAAGCTTCAAAGTGA+AGG | + | chr1.2:11585082-11585101 | None:intergenic | 45.0% | |
| GCTTGGAGATATTAGGTGTC+TGG | - | chr1.2:11584394-11584413 | MS.gene059151:CDS | 45.0% | |
| GTAGCATTCTCTACTTCACG+CGG | + | chr1.2:11584674-11584693 | None:intergenic | 45.0% | |
| TTAGCCGTCCAATCTTGATC+TGG | + | chr1.2:11584816-11584835 | None:intergenic | 45.0% | |
| TTTGAGGGACGATCATAAGG+TGG | - | chr1.2:11584601-11584620 | MS.gene059151:CDS | 45.0% | |
| ! | GACTATCTCGAACTTTTCGG+TGG | + | chr1.2:11585247-11585266 | None:intergenic | 45.0% |
| !! | CACTTTGAAGCTTTCCAGCT+AGG | - | chr1.2:11585083-11585102 | MS.gene059151:CDS | 45.0% |
| !! | TGCTTCAGCTTGCTGTAGTA+TGG | + | chr1.2:11584491-11584510 | None:intergenic | 45.0% |
| !!! | GTGCACCCTTGTTTTACATG+TGG | + | chr1.2:11584348-11584367 | None:intergenic | 45.0% |
| AGGCATGGCTCCAGTTTACA+TGG | - | chr1.2:11585103-11585122 | MS.gene059151:CDS | 50.0% | |
| ATACCTACAGCCTCGAGGTT+GGG | - | chr1.2:11585168-11585187 | MS.gene059151:CDS | 50.0% | |
| CAGTTCCTTCCTATCACCAC+CGG | + | chr1.2:11585312-11585331 | None:intergenic | 50.0% | |
| CATAAGGTGGACATGCACAC+AGG | - | chr1.2:11584614-11584633 | MS.gene059151:CDS | 50.0% | |
| CCAAGCTCTTGTCTACAGGT+CGG | + | chr1.2:11584380-11584399 | None:intergenic | 50.0% | |
| GAACTATACCTACAGCCTCG+AGG | - | chr1.2:11585163-11585182 | MS.gene059151:CDS | 50.0% | |
| GAGCACTCTGATCCAGCATA+AGG | + | chr1.2:11584548-11584567 | None:intergenic | 50.0% | |
| GGTATACAAACCCCACCATC+TGG | + | chr1.2:11585377-11585396 | None:intergenic | 50.0% | |
| TATACCTACAGCCTCGAGGT+TGG | - | chr1.2:11585167-11585186 | MS.gene059151:CDS | 50.0% | |
| TCTCGTCTCCCATGAAACGA+AGG | + | chr1.2:11585133-11585152 | None:intergenic | 50.0% | |
| TGAAGCTTTCCAGCTAGGCA+TGG | - | chr1.2:11585088-11585107 | MS.gene059151:CDS | 50.0% | |
| TTTCATGGGAGACGAGAACG+AGG | - | chr1.2:11585136-11585155 | MS.gene059151:CDS | 50.0% | |
| TTTCTCCGGTGGTGATAGGA+AGG | - | chr1.2:11585304-11585323 | MS.gene059151:CDS | 50.0% | |
| ! | GAACTTTTCGGTGGCTATCG+CGG | + | chr1.2:11585238-11585257 | None:intergenic | 50.0% |
| ! | TAGGTGTCTGGCACTGGAAA+AGG | - | chr1.2:11584406-11584425 | MS.gene059151:CDS | 50.0% |
| ! | TCATTTGGGAGGGTACACCA+CGG | - | chr1.2:11585207-11585226 | MS.gene059151:CDS | 50.0% |
| !! | ATGGCCCTATTTTTCTCCGG+TGG | - | chr1.2:11585293-11585312 | MS.gene059151:CDS | 50.0% |
| AAACTGGAGCCATGCCTAGC+TGG | + | chr1.2:11585100-11585119 | None:intergenic | 55.0% | |
| CCGACCTGTAGACAAGAGCT+TGG | - | chr1.2:11584377-11584396 | MS.gene059151:CDS | 55.0% | |
| CGTCCCTCAAATGTGCAACG+AGG | + | chr1.2:11584592-11584611 | None:intergenic | 55.0% | |
| TACCTACAGCCTCGAGGTTG+GGG | - | chr1.2:11585169-11585188 | MS.gene059151:CDS | 55.0% | |
| TTCCATTTGCCCCAACCTCG+AGG | + | chr1.2:11585181-11585200 | None:intergenic | 55.0% | |
| ! | GGATCAGAGTGCTCTGCTGT+TGG | - | chr1.2:11584554-11584573 | MS.gene059151:CDS | 55.0% |
| AGCCTCGAGGTTGGGGCAAA+TGG | - | chr1.2:11585176-11585195 | MS.gene059151:CDS | 60.0% | |
| TGGCTATCGCGGATACTCCG+TGG | + | chr1.2:11585227-11585246 | None:intergenic | 60.0% | |
| TGGGAGACGAGAACGAGGCT+CGG | - | chr1.2:11585141-11585160 | MS.gene059151:CDS | 60.0% | |
| TGCCCCAACCTCGAGGCTGT+AGG | + | chr1.2:11585174-11585193 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 11584320 | 11585412 | 11584320 | ID=MS.gene059151 |
| chr1.2 | mRNA | 11584320 | 11585412 | 11584320 | ID=MS.gene059151.t1;Parent=MS.gene059151 |
| chr1.2 | exon | 11585026 | 11585412 | 11585026 | ID=MS.gene059151.t1.exon1;Parent=MS.gene059151.t1 |
| chr1.2 | CDS | 11585026 | 11585412 | 11585026 | ID=cds.MS.gene059151.t1;Parent=MS.gene059151.t1 |
| chr1.2 | exon | 11584320 | 11584682 | 11584320 | ID=MS.gene059151.t1.exon2;Parent=MS.gene059151.t1 |
| chr1.2 | CDS | 11584320 | 11584682 | 11584320 | ID=cds.MS.gene059151.t1;Parent=MS.gene059151.t1 |
| Gene Sequence |
| Protein sequence |
