Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37467.t1 | KEH35837.1 | 94.4 | 108 | 2 | 1 | 1 | 108 | 1 | 104 | 1.90E-46 | 194.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37467.t1 | A0A072V1Y9 | 94.4 | 108 | 2 | 1 | 1 | 108 | 1 | 104 | 1.4e-46 | 194.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050864 | MS.gene37467 | 0.820421 | 6.92E-53 | -1.69E-46 |
| MS.gene050904 | MS.gene37467 | -0.800242 | 1.58E-48 | -1.69E-46 |
| MS.gene051718 | MS.gene37467 | 0.802906 | 4.49E-49 | -1.69E-46 |
| MS.gene055251 | MS.gene37467 | 0.815623 | 8.41E-52 | -1.69E-46 |
| MS.gene056998 | MS.gene37467 | 0.80174 | 7.81E-49 | -1.69E-46 |
| MS.gene058382 | MS.gene37467 | 0.809184 | 2.15E-50 | -1.69E-46 |
| MS.gene058386 | MS.gene37467 | 0.800582 | 1.35E-48 | -1.69E-46 |
| MS.gene06042 | MS.gene37467 | 0.848278 | 6.79E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37467.t1 | MTR_3g103330 | 94.444 | 108 | 2 | 1 | 1 | 108 | 1 | 104 | 1.04e-69 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCGAGTCAAAGCTCTTC+TGG | 0.318499 | 3.4:+90961181 | MS.gene37467:CDS |
| CCTTCTCCTGCTGGTGGAAC+CGG | 0.386344 | 3.4:-90961383 | None:intergenic |
| GGTTGCAGTGGAAAGCTTGA+TGG | 0.410694 | 3.4:+90961202 | MS.gene37467:CDS |
| CGCCATGACGGTGGTGTAGC+CGG | 0.417162 | 3.4:+90961364 | MS.gene37467:CDS |
| TTTACCGGTCCTTCTCCTGC+TGG | 0.444782 | 3.4:-90961392 | None:intergenic |
| GCAGGAGAAGGACCGGTAAA+GGG | 0.444911 | 3.4:+90961395 | MS.gene37467:CDS |
| AGCAGGAGAAGGACCGGTAA+AGG | 0.449501 | 3.4:+90961394 | MS.gene37467:CDS |
| GAAACCACTGCAGGTGGAAG+AGG | 0.474000 | 3.4:-90961134 | None:intergenic |
| TTCATCTAGATCATTGTTCG+TGG | 0.475117 | 3.4:-90961435 | None:intergenic |
| GAACATCAATGGCAACTCAT+TGG | 0.481610 | 3.4:-90961326 | None:intergenic |
| GTGTAGCCGGTTCCACCAGC+AGG | 0.482386 | 3.4:+90961377 | MS.gene37467:CDS |
| GTTGATCAATGGTGTCACTA+TGG | 0.489333 | 3.4:+90961237 | MS.gene37467:CDS |
| ACCGGTCCTTCTCCTGCTGG+TGG | 0.523534 | 3.4:-90961389 | None:intergenic |
| TTACCATCGTTGAACATCAA+TGG | 0.525954 | 3.4:-90961337 | None:intergenic |
| ATCTTCTCCGTCTTCTTCTG+TGG | 0.526120 | 3.4:-90961302 | None:intergenic |
| ACTACCTCTTCCACCTGCAG+TGG | 0.560557 | 3.4:+90961130 | MS.gene37467:CDS |
| CAAAGCTCTTCTGGTTGCAG+TGG | 0.566167 | 3.4:+90961190 | MS.gene37467:CDS |
| TCCACCAGCAGGAGAAGGAC+CGG | 0.569119 | 3.4:+90961388 | MS.gene37467:CDS |
| CCGGTTCCACCAGCAGGAGA+AGG | 0.578852 | 3.4:+90961383 | MS.gene37467:CDS |
| TCGAAGTATTCGATGATGAT+CGG | 0.586920 | 3.4:-90961165 | None:intergenic |
| GTAGCTATGTGGTTGATCAA+TGG | 0.596285 | 3.4:+90961226 | MS.gene37467:CDS |
| GAAGTTGAAGAAACCACTGC+AGG | 0.609331 | 3.4:-90961143 | None:intergenic |
| AACCGGCTACACCACCGTCA+TGG | 0.612729 | 3.4:-90961366 | None:intergenic |
| GTTGAAGAAACCACTGCAGG+TGG | 0.625992 | 3.4:-90961140 | None:intergenic |
| GGTAATCTCCGCCATGACGG+TGG | 0.628227 | 3.4:+90961355 | MS.gene37467:CDS |
| CGCATCGCCACAGAAGAAGA+CGG | 0.672339 | 3.4:+90961295 | MS.gene37467:CDS |
| GATGGTAATCTCCGCCATGA+CGG | 0.678032 | 3.4:+90961352 | MS.gene37467:CDS |
| AGCTTGATGGAGTAGCTATG+TGG | 0.699449 | 3.4:+90961215 | MS.gene37467:CDS |
| CGGCTACACCACCGTCATGG+CGG | 0.715775 | 3.4:-90961363 | None:intergenic |
| TTGCCATTGATGTTCAACGA+TGG | 0.744804 | 3.4:+90961334 | MS.gene37467:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTACCATCGTTGAACATCAA+TGG | - | chr3.4:90961340-90961359 | None:intergenic | 35.0% | |
| ! | TACACTTTTCTTCCCTTTAC+CGG | - | chr3.4:90961410-90961429 | None:intergenic | 35.0% |
| ! | TCGAAGTATTCGATGATGAT+CGG | - | chr3.4:90961168-90961187 | None:intergenic | 35.0% |
| GAACATCAATGGCAACTCAT+TGG | - | chr3.4:90961329-90961348 | None:intergenic | 40.0% | |
| GTAGCTATGTGGTTGATCAA+TGG | + | chr3.4:90961226-90961245 | MS.gene37467:CDS | 40.0% | |
| TTGCCATTGATGTTCAACGA+TGG | + | chr3.4:90961334-90961353 | MS.gene37467:CDS | 40.0% | |
| !! | GTTGATCAATGGTGTCACTA+TGG | + | chr3.4:90961237-90961256 | MS.gene37467:CDS | 40.0% |
| ACTTCGAGTCAAAGCTCTTC+TGG | + | chr3.4:90961181-90961200 | MS.gene37467:CDS | 45.0% | |
| AGCTTGATGGAGTAGCTATG+TGG | + | chr3.4:90961215-90961234 | MS.gene37467:CDS | 45.0% | |
| ATCTTCTCCGTCTTCTTCTG+TGG | - | chr3.4:90961305-90961324 | None:intergenic | 45.0% | |
| GAAGTTGAAGAAACCACTGC+AGG | - | chr3.4:90961146-90961165 | None:intergenic | 45.0% | |
| GATGGTAATCTCCGCCATGA+CGG | + | chr3.4:90961352-90961371 | MS.gene37467:CDS | 50.0% | |
| GGTTGCAGTGGAAAGCTTGA+TGG | + | chr3.4:90961202-90961221 | MS.gene37467:CDS | 50.0% | |
| GTTGAAGAAACCACTGCAGG+TGG | - | chr3.4:90961143-90961162 | None:intergenic | 50.0% | |
| ! | CAAAGCTCTTCTGGTTGCAG+TGG | + | chr3.4:90961190-90961209 | MS.gene37467:CDS | 50.0% |
| ACTACCTCTTCCACCTGCAG+TGG | + | chr3.4:90961130-90961149 | MS.gene37467:CDS | 55.0% | |
| AGCAGGAGAAGGACCGGTAA+AGG | + | chr3.4:90961394-90961413 | MS.gene37467:CDS | 55.0% | |
| CGCATCGCCACAGAAGAAGA+CGG | + | chr3.4:90961295-90961314 | MS.gene37467:CDS | 55.0% | |
| GAAACCACTGCAGGTGGAAG+AGG | - | chr3.4:90961137-90961156 | None:intergenic | 55.0% | |
| GCAGGAGAAGGACCGGTAAA+GGG | + | chr3.4:90961395-90961414 | MS.gene37467:CDS | 55.0% | |
| TTTACCGGTCCTTCTCCTGC+TGG | - | chr3.4:90961395-90961414 | None:intergenic | 55.0% | |
| AACCGGCTACACCACCGTCA+TGG | - | chr3.4:90961369-90961388 | None:intergenic | 60.0% | |
| CCTTCTCCTGCTGGTGGAAC+CGG | - | chr3.4:90961386-90961405 | None:intergenic | 60.0% | |
| GGTAATCTCCGCCATGACGG+TGG | + | chr3.4:90961355-90961374 | MS.gene37467:CDS | 60.0% | |
| TCCACCAGCAGGAGAAGGAC+CGG | + | chr3.4:90961388-90961407 | MS.gene37467:CDS | 60.0% | |
| ACCGGTCCTTCTCCTGCTGG+TGG | - | chr3.4:90961392-90961411 | None:intergenic | 65.0% | |
| CCGGTTCCACCAGCAGGAGA+AGG | + | chr3.4:90961383-90961402 | MS.gene37467:CDS | 65.0% | |
| CGCCATGACGGTGGTGTAGC+CGG | + | chr3.4:90961364-90961383 | MS.gene37467:CDS | 65.0% | |
| CGGCTACACCACCGTCATGG+CGG | - | chr3.4:90961366-90961385 | None:intergenic | 65.0% | |
| ! | GTGTAGCCGGTTCCACCAGC+AGG | + | chr3.4:90961377-90961396 | MS.gene37467:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 90961121 | 90961447 | 90961121 | ID=MS.gene37467 |
| chr3.4 | mRNA | 90961121 | 90961447 | 90961121 | ID=MS.gene37467.t1;Parent=MS.gene37467 |
| chr3.4 | exon | 90961121 | 90961447 | 90961121 | ID=MS.gene37467.t1.exon1;Parent=MS.gene37467.t1 |
| chr3.4 | CDS | 90961121 | 90961447 | 90961121 | ID=cds.MS.gene37467.t1;Parent=MS.gene37467.t1 |
| Gene Sequence |
| Protein sequence |
