Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37696.t1 | XP_013469748.1 | 88.4 | 199 | 21 | 1 | 1 | 199 | 1 | 197 | 1.00E-85 | 326.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37696.t1 | P11432 | 78.9 | 199 | 39 | 1 | 1 | 199 | 1 | 196 | 1.4e-78 | 293.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37696.t1 | A0A072VQ49 | 88.4 | 199 | 21 | 1 | 1 | 199 | 1 | 197 | 7.4e-86 | 326.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049170 | MS.gene37696 | 0.807414 | 5.11E-50 | -1.69E-46 |
| MS.gene05081 | MS.gene37696 | 0.819244 | 1.29E-52 | -1.69E-46 |
| MS.gene051734 | MS.gene37696 | 0.825088 | 5.67E-54 | -1.69E-46 |
| MS.gene052188 | MS.gene37696 | 0.807469 | 4.98E-50 | -1.69E-46 |
| MS.gene052469 | MS.gene37696 | 0.852195 | 5.41E-61 | -1.69E-46 |
| MS.gene052870 | MS.gene37696 | 0.842773 | 2.11E-58 | -1.69E-46 |
| MS.gene052872 | MS.gene37696 | 0.836567 | 8.68E-57 | -1.69E-46 |
| MS.gene053824 | MS.gene37696 | 0.805159 | 1.53E-49 | -1.69E-46 |
| MS.gene05494 | MS.gene37696 | 0.814464 | 1.52E-51 | -1.69E-46 |
| MS.gene055224 | MS.gene37696 | 0.805522 | 1.28E-49 | -1.69E-46 |
| MS.gene055539 | MS.gene37696 | 0.836575 | 8.64E-57 | -1.69E-46 |
| MS.gene055741 | MS.gene37696 | 0.811658 | 6.27E-51 | -1.69E-46 |
| MS.gene055744 | MS.gene37696 | 0.814392 | 1.58E-51 | -1.69E-46 |
| MS.gene057019 | MS.gene37696 | 0.800826 | 1.20E-48 | -1.69E-46 |
| MS.gene059717 | MS.gene37696 | 0.834624 | 2.69E-56 | -1.69E-46 |
| MS.gene061154 | MS.gene37696 | 0.800434 | 1.44E-48 | -1.69E-46 |
| MS.gene061231 | MS.gene37696 | 0.809089 | 2.25E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37696.t1 | MTR_1g102570 | 90.556 | 180 | 15 | 1 | 20 | 199 | 21 | 198 | 7.32e-109 | 310 |
| MS.gene37696.t1 | MTR_1g102800 | 90.556 | 180 | 15 | 1 | 20 | 199 | 20 | 197 | 1.90e-108 | 309 |
| MS.gene37696.t1 | MTR_1g102780 | 90.000 | 180 | 18 | 0 | 20 | 199 | 21 | 200 | 1.05e-103 | 297 |
| MS.gene37696.t1 | MTR_1g102810 | 74.317 | 183 | 33 | 2 | 17 | 199 | 14 | 182 | 4.97e-94 | 272 |
| MS.gene37696.t1 | MTR_1g102830 | 76.111 | 180 | 29 | 2 | 20 | 199 | 21 | 186 | 5.39e-94 | 272 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37696.t1 | AT3G22840 | 49.528 | 212 | 77 | 4 | 1 | 199 | 1 | 195 | 7.84e-61 | 188 |
| MS.gene37696.t1 | AT4G14690 | 52.000 | 200 | 88 | 4 | 1 | 199 | 1 | 193 | 6.08e-58 | 181 |
Find 51 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTCAATGAGCACCATTTC+TGG | 0.210857 | 1.1:+73765531 | MS.gene37696:CDS |
| TGTGATGGTGGTGGTGGTTT+TGG | 0.242663 | 1.1:-73765833 | None:intergenic |
| TGGTGGCATCCCATGGTTCT+TGG | 0.273833 | 1.1:+73766139 | MS.gene37696:CDS |
| GGTGATGATGATGGTGTAAT+TGG | 0.282689 | 1.1:-73765812 | None:intergenic |
| GGTGGCATCCCATGGTTCTT+GGG | 0.286207 | 1.1:+73766140 | MS.gene37696:CDS |
| GGTTTGTTTGATCAAATATC+TGG | 0.314167 | 1.1:+73766116 | MS.gene37696:CDS |
| TATGTTGGGTTTCATTGCTT+TGG | 0.354498 | 1.1:+73766280 | MS.gene37696:CDS |
| CAGAAGGGATGTTGCTGAAT+TGG | 0.358772 | 1.1:-73765569 | None:intergenic |
| AATGGAAGGTTGGCTATGAT+TGG | 0.393839 | 1.1:+73766053 | MS.gene37696:CDS |
| GGCCTTTACAGAGTATGTTA+AGG | 0.395379 | 1.1:+73766301 | MS.gene37696:CDS |
| GCCTTTACAGAGTATGTTAA+GGG | 0.401917 | 1.1:+73766302 | MS.gene37696:CDS |
| CCATTGATTCTCTCAGGTGC+TGG | 0.411549 | 1.1:-73766035 | None:intergenic |
| AACCTTCCATTGATTCTCTC+AGG | 0.436061 | 1.1:-73766041 | None:intergenic |
| TGTCCTCAGATGCAGAACTA+TGG | 0.436951 | 1.1:+73766243 | MS.gene37696:CDS |
| GAATGGAAGAATTGCTATGT+TGG | 0.442937 | 1.1:+73766265 | MS.gene37696:CDS |
| GTGATGATGATGGTGTAATT+GGG | 0.448255 | 1.1:-73765811 | None:intergenic |
| GACTCTAGACTTGCCAGAAA+TGG | 0.452328 | 1.1:-73765544 | None:intergenic |
| TGCCATCAAGTCTGAAAACT+TGG | 0.455106 | 1.1:-73766003 | None:intergenic |
| GCTGATTGTGATGGTGGTGG+TGG | 0.464676 | 1.1:-73765839 | None:intergenic |
| TACCTTTGGTGCGCGAGTAT+AGG | 0.465801 | 1.1:-73765861 | None:intergenic |
| TCAGACTTGATGGCATTTGG+TGG | 0.470606 | 1.1:+73766011 | MS.gene37696:CDS |
| GTGGCATCCCATGGTTCTTG+GGG | 0.484949 | 1.1:+73766141 | MS.gene37696:CDS |
| CAGACTTGATGGCATTTGGT+GGG | 0.492385 | 1.1:+73766012 | MS.gene37696:CDS |
| AGTGTTGAGTCTAAGTCAAA+AGG | 0.509995 | 1.1:+73766215 | MS.gene37696:CDS |
| AATGGAAGAATTGCTATGTT+GGG | 0.512582 | 1.1:+73766266 | MS.gene37696:CDS |
| CCAGCACCTGAGAGAATCAA+TGG | 0.516939 | 1.1:+73766035 | MS.gene37696:CDS |
| CACTTGTCCCCAAGAACCAT+GGG | 0.531790 | 1.1:-73766148 | None:intergenic |
| AACCTTAATTACCTCAGCCA+TGG | 0.545130 | 1.1:-73765637 | None:intergenic |
| GGTGGAGATAGCAAATGGAC+AGG | 0.547189 | 1.1:+73766094 | MS.gene37696:CDS |
| ATTCCATAGTTCTGCATCTG+AGG | 0.548696 | 1.1:-73766246 | None:intergenic |
| ATAGCATTCCTTCTGAAGCT+AGG | 0.551512 | 1.1:-73765600 | None:intergenic |
| ACCCTTAACATACTCTGTAA+AGG | 0.552111 | 1.1:-73766303 | None:intergenic |
| AGCCTATACTCGCGCACCAA+AGG | 0.555920 | 1.1:+73765859 | MS.gene37696:CDS |
| TGAGAGAATCAATGGAAGGT+TGG | 0.560259 | 1.1:+73766043 | MS.gene37696:CDS |
| TTTGATCAAATATCTGGTGG+TGG | 0.563790 | 1.1:+73766122 | MS.gene37696:CDS |
| GATGGTGTAATTGGGTCTAC+AGG | 0.568013 | 1.1:-73765803 | None:intergenic |
| TAGGCTGATTGTGATGGTGG+TGG | 0.569312 | 1.1:-73765842 | None:intergenic |
| TTGTTTGATCAAATATCTGG+TGG | 0.570609 | 1.1:+73766119 | MS.gene37696:CDS |
| AAGCTAGGCATGTACACAGA+AGG | 0.579754 | 1.1:-73765585 | None:intergenic |
| TAGACTCAACACTAACACCT+TGG | 0.586516 | 1.1:-73766205 | None:intergenic |
| TATCTGGTGGTGGCATCCCA+TGG | 0.587584 | 1.1:+73766132 | MS.gene37696:CDS |
| CGAGTATAGGCTGATTGTGA+TGG | 0.595009 | 1.1:-73765848 | None:intergenic |
| TCAGATGCAGAACTATGGAA+TGG | 0.597938 | 1.1:+73766248 | MS.gene37696:CDS |
| TATCTTGAAAGTTCGATCCA+TGG | 0.599815 | 1.1:+73765620 | MS.gene37696:CDS |
| ACACTTGTCCCCAAGAACCA+TGG | 0.606138 | 1.1:-73766149 | None:intergenic |
| AGCTAGGCATGTACACAGAA+GGG | 0.614155 | 1.1:-73765584 | None:intergenic |
| GAAAGTTCGATCCATGGCTG+AGG | 0.622095 | 1.1:+73765626 | MS.gene37696:CDS |
| TGTACATGCCTAGCTTCAGA+AGG | 0.622254 | 1.1:+73765592 | MS.gene37696:CDS |
| GTATAGGCTGATTGTGATGG+TGG | 0.641892 | 1.1:-73765845 | None:intergenic |
| GTGGAGATAGCAAATGGACA+GGG | 0.645295 | 1.1:+73766095 | MS.gene37696:CDS |
| CACCTGAGAGAATCAATGGA+AGG | 0.646646 | 1.1:+73766039 | MS.gene37696:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATAAACTAAATTTAATTA+AGG | + | chr1.1:73765662-73765681 | MS.gene37696:intron | 10.0% |
| !!! | TTTATGTATAGTATTGATTT+TGG | + | chr1.1:73765922-73765941 | MS.gene37696:intron | 15.0% |
| !! | ACATAATATGTTTATGATTC+AGG | + | chr1.1:73765754-73765773 | MS.gene37696:intron | 20.0% |
| !! | GAATCATAAACATATTATGT+TGG | - | chr1.1:73765754-73765773 | None:intergenic | 20.0% |
| ! | ATCGTCATTATTCAATGAAA+GGG | - | chr1.1:73765963-73765982 | None:intergenic | 25.0% |
| ! | TATCGTCATTATTCAATGAA+AGG | - | chr1.1:73765964-73765983 | None:intergenic | 25.0% |
| AATGGAAGAATTGCTATGTT+GGG | + | chr1.1:73766266-73766285 | MS.gene37696:CDS | 30.0% | |
| TTGTTTGATCAAATATCTGG+TGG | + | chr1.1:73766119-73766138 | MS.gene37696:CDS | 30.0% | |
| ! | GGTTTGTTTGATCAAATATC+TGG | + | chr1.1:73766116-73766135 | MS.gene37696:CDS | 30.0% |
| !! | TCTTTGATTCCATTTTTCCA+AGG | + | chr1.1:73766188-73766207 | MS.gene37696:CDS | 30.0% |
| ACACTAACACCTTGGAAAAA+TGG | - | chr1.1:73766200-73766219 | None:intergenic | 35.0% | |
| ACCCTTAACATACTCTGTAA+AGG | - | chr1.1:73766306-73766325 | None:intergenic | 35.0% | |
| AGAACAGTTATGCTTACCTT+TGG | - | chr1.1:73765878-73765897 | None:intergenic | 35.0% | |
| GAATGGAAGAATTGCTATGT+TGG | + | chr1.1:73766265-73766284 | MS.gene37696:CDS | 35.0% | |
| GCCTTTACAGAGTATGTTAA+GGG | + | chr1.1:73766302-73766321 | MS.gene37696:CDS | 35.0% | |
| TATCTTGAAAGTTCGATCCA+TGG | + | chr1.1:73765620-73765639 | MS.gene37696:CDS | 35.0% | |
| TATGTTGGGTTTCATTGCTT+TGG | + | chr1.1:73766280-73766299 | MS.gene37696:CDS | 35.0% | |
| TGAGCAAAAAGAGCAATCAA+AGG | + | chr1.1:73765778-73765797 | MS.gene37696:CDS | 35.0% | |
| TTTGATCAAATATCTGGTGG+TGG | + | chr1.1:73766122-73766141 | MS.gene37696:CDS | 35.0% | |
| ! | AGTGTTGAGTCTAAGTCAAA+AGG | + | chr1.1:73766215-73766234 | MS.gene37696:CDS | 35.0% |
| ! | GGTGATGATGATTGTGTAAT+CGG | - | chr1.1:73765733-73765752 | None:intergenic | 35.0% |
| !! | GTGATGATGATGGTGTAATT+GGG | - | chr1.1:73765814-73765833 | None:intergenic | 35.0% |
| !! | TTTTCAGACTTGATGGCATT+TGG | + | chr1.1:73766008-73766027 | MS.gene37696:CDS | 35.0% |
| !!! | ATTGGTTTTGTAGCAGCAAT+GGG | + | chr1.1:73766071-73766090 | MS.gene37696:CDS | 35.0% |
| AACCTTAATTACCTCAGCCA+TGG | - | chr1.1:73765640-73765659 | None:intergenic | 40.0% | |
| ATCCATGGCTGAGGTAATTA+AGG | + | chr1.1:73765635-73765654 | MS.gene37696:intron | 40.0% | |
| GGCCTTTACAGAGTATGTTA+AGG | + | chr1.1:73766301-73766320 | MS.gene37696:CDS | 40.0% | |
| TAGACTCAACACTAACACCT+TGG | - | chr1.1:73766208-73766227 | None:intergenic | 40.0% | |
| TCAGATGCAGAACTATGGAA+TGG | + | chr1.1:73766248-73766267 | MS.gene37696:CDS | 40.0% | |
| TGAGAGAATCAATGGAAGGT+TGG | + | chr1.1:73766043-73766062 | MS.gene37696:CDS | 40.0% | |
| TGCCATCAAGTCTGAAAACT+TGG | - | chr1.1:73766006-73766025 | None:intergenic | 40.0% | |
| ! | AATGGAAGGTTGGCTATGAT+TGG | + | chr1.1:73766053-73766072 | MS.gene37696:CDS | 40.0% |
| ! | ATTCCATAGTTCTGCATCTG+AGG | - | chr1.1:73766249-73766268 | None:intergenic | 40.0% |
| ! | CACCAAGTTTTCAGACTTGA+TGG | + | chr1.1:73766001-73766020 | MS.gene37696:CDS | 40.0% |
| !! | AACCTTCCATTGATTCTCTC+AGG | - | chr1.1:73766044-73766063 | None:intergenic | 40.0% |
| !! | ATAGCATTCCTTCTGAAGCT+AGG | - | chr1.1:73765603-73765622 | None:intergenic | 40.0% |
| !! | GGTGATGATGATGGTGTAAT+TGG | - | chr1.1:73765815-73765834 | None:intergenic | 40.0% |
| !!! | GATTGGTTTTGTAGCAGCAA+TGG | + | chr1.1:73766070-73766089 | MS.gene37696:CDS | 40.0% |
| !!! | TTGGTTTTGTAGCAGCAATG+GGG | + | chr1.1:73766072-73766091 | MS.gene37696:CDS | 40.0% |
| AAGCTAGGCATGTACACAGA+AGG | - | chr1.1:73765588-73765607 | None:intergenic | 45.0% | |
| AGCTAGGCATGTACACAGAA+GGG | - | chr1.1:73765587-73765606 | None:intergenic | 45.0% | |
| AGCTCAATGAGCACCATTTC+TGG | + | chr1.1:73765531-73765550 | MS.gene37696:CDS | 45.0% | |
| CACCTGAGAGAATCAATGGA+AGG | + | chr1.1:73766039-73766058 | MS.gene37696:CDS | 45.0% | |
| CAGAAGGGATGTTGCTGAAT+TGG | - | chr1.1:73765572-73765591 | None:intergenic | 45.0% | |
| CGAGTATAGGCTGATTGTGA+TGG | - | chr1.1:73765851-73765870 | None:intergenic | 45.0% | |
| GACTCTAGACTTGCCAGAAA+TGG | - | chr1.1:73765547-73765566 | None:intergenic | 45.0% | |
| GATGGTGTAATTGGGTCTAC+AGG | - | chr1.1:73765806-73765825 | None:intergenic | 45.0% | |
| GTATAGGCTGATTGTGATGG+TGG | - | chr1.1:73765848-73765867 | None:intergenic | 45.0% | |
| GTGGAGATAGCAAATGGACA+GGG | + | chr1.1:73766095-73766114 | MS.gene37696:CDS | 45.0% | |
| TGTACATGCCTAGCTTCAGA+AGG | + | chr1.1:73765592-73765611 | MS.gene37696:CDS | 45.0% | |
| TGTCCTCAGATGCAGAACTA+TGG | + | chr1.1:73766243-73766262 | MS.gene37696:CDS | 45.0% | |
| ! | CAGACTTGATGGCATTTGGT+GGG | + | chr1.1:73766012-73766031 | MS.gene37696:CDS | 45.0% |
| ! | TCAGACTTGATGGCATTTGG+TGG | + | chr1.1:73766011-73766030 | MS.gene37696:CDS | 45.0% |
| !!! | GGTGGTTTTGGTGATGATGA+TGG | - | chr1.1:73765824-73765843 | None:intergenic | 45.0% |
| !!! | TGGTTTTGTAGCAGCAATGG+GGG | + | chr1.1:73766073-73766092 | MS.gene37696:CDS | 45.0% |
| ACACTTGTCCCCAAGAACCA+TGG | - | chr1.1:73766152-73766171 | None:intergenic | 50.0% | |
| ATGGGGGTGGAGATAGCAAA+TGG | + | chr1.1:73766089-73766108 | MS.gene37696:CDS | 50.0% | |
| CACTTGTCCCCAAGAACCAT+GGG | - | chr1.1:73766151-73766170 | None:intergenic | 50.0% | |
| CCAGCACCTGAGAGAATCAA+TGG | + | chr1.1:73766035-73766054 | MS.gene37696:CDS | 50.0% | |
| GAAAGTTCGATCCATGGCTG+AGG | + | chr1.1:73765626-73765645 | MS.gene37696:CDS | 50.0% | |
| GGTGGAGATAGCAAATGGAC+AGG | + | chr1.1:73766094-73766113 | MS.gene37696:CDS | 50.0% | |
| ! | TACCTTTGGTGCGCGAGTAT+AGG | - | chr1.1:73765864-73765883 | None:intergenic | 50.0% |
| ! | TTTTGTAGCAGCAATGGGGG+TGG | + | chr1.1:73766076-73766095 | MS.gene37696:CDS | 50.0% |
| !! | CCATTGATTCTCTCAGGTGC+TGG | - | chr1.1:73766038-73766057 | None:intergenic | 50.0% |
| !! | TAGGCTGATTGTGATGGTGG+TGG | - | chr1.1:73765845-73765864 | None:intergenic | 50.0% |
| !!! | TGTGATGGTGGTGGTGGTTT+TGG | - | chr1.1:73765836-73765855 | None:intergenic | 50.0% |
| AGCCTATACTCGCGCACCAA+AGG | + | chr1.1:73765859-73765878 | MS.gene37696:CDS | 55.0% | |
| GGTGGCATCCCATGGTTCTT+GGG | + | chr1.1:73766140-73766159 | MS.gene37696:CDS | 55.0% | |
| GTGGCATCCCATGGTTCTTG+GGG | + | chr1.1:73766141-73766160 | MS.gene37696:CDS | 55.0% | |
| TGGTGGCATCCCATGGTTCT+TGG | + | chr1.1:73766139-73766158 | MS.gene37696:CDS | 55.0% | |
| !! | GCTGATTGTGATGGTGGTGG+TGG | - | chr1.1:73765842-73765861 | None:intergenic | 55.0% |
| !! | TATCTGGTGGTGGCATCCCA+TGG | + | chr1.1:73766132-73766151 | MS.gene37696:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 73765498 | 73766340 | 73765498 | ID=MS.gene37696 |
| chr1.1 | mRNA | 73765498 | 73766340 | 73765498 | ID=MS.gene37696.t1;Parent=MS.gene37696 |
| chr1.1 | exon | 73765498 | 73765647 | 73765498 | ID=MS.gene37696.t1.exon1;Parent=MS.gene37696.t1 |
| chr1.1 | CDS | 73765498 | 73765647 | 73765498 | ID=cds.MS.gene37696.t1;Parent=MS.gene37696.t1 |
| chr1.1 | exon | 73765776 | 73765880 | 73765776 | ID=MS.gene37696.t1.exon2;Parent=MS.gene37696.t1 |
| chr1.1 | CDS | 73765776 | 73765880 | 73765776 | ID=cds.MS.gene37696.t1;Parent=MS.gene37696.t1 |
| chr1.1 | exon | 73765996 | 73766340 | 73765996 | ID=MS.gene37696.t1.exon3;Parent=MS.gene37696.t1 |
| chr1.1 | CDS | 73765996 | 73766340 | 73765996 | ID=cds.MS.gene37696.t1;Parent=MS.gene37696.t1 |
| Gene Sequence |
| Protein sequence |
