Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38740.t1 | XP_003603001.1 | 99.6 | 237 | 1 | 0 | 1 | 237 | 1 | 237 | 4.70E-130 | 473.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38740.t1 | O22227 | 44.8 | 172 | 82 | 4 | 72 | 235 | 130 | 296 | 3.5e-33 | 143.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38740.t1 | G7J5T6 | 99.6 | 237 | 1 | 0 | 1 | 237 | 1 | 237 | 3.4e-130 | 473.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053074 | MS.gene38740 | 0.806073 | 9.82E-50 | -1.69E-46 |
| MS.gene054473 | MS.gene38740 | 0.805638 | 1.21E-49 | -1.69E-46 |
| MS.gene054618 | MS.gene38740 | 0.927902 | 6.14E-92 | -1.69E-46 |
| MS.gene059228 | MS.gene38740 | 0.807836 | 4.16E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene38740 | 0.830193 | 3.37E-55 | -1.69E-46 |
| MS.gene061049 | MS.gene38740 | 0.808493 | 3.01E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38740.t1 | MTR_3g101300 | 99.578 | 237 | 1 | 0 | 1 | 237 | 1 | 237 | 3.73e-180 | 493 |
| MS.gene38740.t1 | MTR_8g040690 | 41.564 | 243 | 120 | 9 | 2 | 236 | 19 | 247 | 2.79e-53 | 172 |
| MS.gene38740.t1 | MTR_3g094830 | 45.098 | 204 | 96 | 7 | 41 | 235 | 62 | 258 | 5.34e-52 | 169 |
| MS.gene38740.t1 | MTR_8g040680 | 43.689 | 206 | 103 | 6 | 36 | 236 | 60 | 257 | 1.95e-51 | 168 |
| MS.gene38740.t1 | MTR_2g054380 | 49.102 | 167 | 76 | 3 | 72 | 235 | 109 | 269 | 2.48e-48 | 160 |
| MS.gene38740.t1 | MTR_4g112370 | 46.821 | 173 | 77 | 6 | 69 | 235 | 96 | 259 | 1.58e-44 | 150 |
| MS.gene38740.t1 | MTR_2g055990 | 45.000 | 180 | 87 | 6 | 60 | 235 | 87 | 258 | 4.22e-43 | 146 |
| MS.gene38740.t1 | MTR_1g053130 | 45.665 | 173 | 79 | 4 | 72 | 235 | 117 | 283 | 2.08e-39 | 137 |
| MS.gene38740.t1 | MTR_3g086740 | 42.458 | 179 | 86 | 7 | 65 | 236 | 75 | 243 | 6.22e-38 | 132 |
| MS.gene38740.t1 | MTR_7g064810 | 41.667 | 180 | 87 | 6 | 60 | 236 | 59 | 223 | 3.16e-35 | 125 |
| MS.gene38740.t1 | MTR_8g089865 | 40.000 | 170 | 89 | 7 | 73 | 236 | 59 | 221 | 3.10e-33 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38740.t1 | AT5G42680 | 61.885 | 244 | 80 | 5 | 1 | 237 | 1 | 238 | 5.50e-101 | 293 |
| MS.gene38740.t1 | AT5G42680 | 61.885 | 244 | 80 | 5 | 1 | 237 | 1 | 238 | 5.50e-101 | 293 |
| MS.gene38740.t1 | AT4G39610 | 46.581 | 234 | 99 | 10 | 20 | 236 | 40 | 264 | 2.40e-58 | 186 |
| MS.gene38740.t1 | AT5G06990 | 43.333 | 240 | 114 | 8 | 8 | 236 | 33 | 261 | 4.56e-54 | 175 |
| MS.gene38740.t1 | AT2G21990 | 47.291 | 203 | 92 | 7 | 41 | 236 | 58 | 252 | 1.32e-53 | 173 |
| MS.gene38740.t1 | AT3G25640 | 50.000 | 172 | 72 | 7 | 73 | 235 | 100 | 266 | 8.87e-49 | 161 |
| MS.gene38740.t1 | AT3G25640 | 50.000 | 172 | 72 | 7 | 73 | 235 | 126 | 292 | 1.65e-48 | 161 |
| MS.gene38740.t1 | AT2G41660 | 44.509 | 173 | 83 | 4 | 71 | 235 | 129 | 296 | 2.22e-44 | 151 |
| MS.gene38740.t1 | AT5G23100 | 42.051 | 195 | 79 | 5 | 69 | 235 | 88 | 276 | 3.33e-40 | 139 |
| MS.gene38740.t1 | AT2G37880 | 43.617 | 188 | 88 | 8 | 55 | 236 | 70 | 245 | 7.82e-40 | 137 |
| MS.gene38740.t1 | AT1G21050 | 41.176 | 170 | 81 | 6 | 73 | 236 | 89 | 245 | 5.97e-36 | 127 |
| MS.gene38740.t1 | AT1G21050 | 41.176 | 170 | 81 | 6 | 73 | 236 | 125 | 281 | 1.06e-35 | 128 |
| MS.gene38740.t1 | AT5G65340 | 40.571 | 175 | 86 | 5 | 72 | 236 | 84 | 250 | 3.72e-32 | 118 |
| MS.gene38740.t1 | AT1G76610 | 38.235 | 170 | 84 | 6 | 70 | 236 | 75 | 226 | 1.22e-29 | 110 |
| MS.gene38740.t1 | AT2G22460 | 36.145 | 166 | 95 | 4 | 71 | 234 | 85 | 241 | 9.32e-26 | 101 |
Find 46 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTAGATTTGAAAGAGTAAT+TGG | 0.213989 | 3.3:-83943118 | MS.gene38740:CDS |
| TGAAAGAGTAATTGGTTCTA+AGG | 0.266032 | 3.3:-83943110 | MS.gene38740:CDS |
| GAAAAGTTGGGTATTCAATT+AGG | 0.280770 | 3.3:-83943273 | MS.gene38740:CDS |
| AGAGGTGTGTCCATGGGAGC+TGG | 0.305516 | 3.3:-83943205 | MS.gene38740:CDS |
| CCATTATTAATATTATTATC+AGG | 0.336954 | 3.3:+83943061 | None:intergenic |
| GCCACAACAGGAACAATCTT+TGG | 0.337368 | 3.3:-83943499 | MS.gene38740:CDS |
| CCTGATAATAATATTAATAA+TGG | 0.346292 | 3.3:-83943061 | MS.gene38740:CDS |
| TATTGTAATGGAAGAAAAGT+TGG | 0.347703 | 3.3:-83943286 | MS.gene38740:CDS |
| AGTTGTTGGAAGAGTTTGTT+TGG | 0.358226 | 3.3:-83943315 | MS.gene38740:CDS |
| TGCAAAATGGTAGCACTATT+AGG | 0.361254 | 3.3:-83943553 | MS.gene38740:CDS |
| GTTAACATCTGGTTGCAAAA+TGG | 0.363174 | 3.3:-83943566 | MS.gene38740:CDS |
| TTCAATTAGGAGGAAACAAA+TGG | 0.393295 | 3.3:-83943260 | MS.gene38740:CDS |
| TACCTCTCATATAAGTCATT+TGG | 0.396969 | 3.3:+83943137 | None:intergenic |
| GTTTGGGCTGTTTATTGTAA+TGG | 0.399156 | 3.3:-83943298 | MS.gene38740:CDS |
| CTCTTCCCTATGTTAACATC+TGG | 0.399968 | 3.3:-83943577 | MS.gene38740:CDS |
| ACAATCTTTGGTTACAGAAA+AGG | 0.421379 | 3.3:-83943487 | MS.gene38740:CDS |
| CGAAGGAGTGATGCTATTGC+TGG | 0.423322 | 3.3:+83943664 | None:intergenic |
| ATTGTAATGGAAGAAAAGTT+GGG | 0.435261 | 3.3:-83943285 | MS.gene38740:CDS |
| AACGAATAAGAAGAAGTTGT+TGG | 0.437700 | 3.3:-83943329 | MS.gene38740:CDS |
| GAGGTGTGTCCATGGGAGCT+GGG | 0.448845 | 3.3:-83943204 | MS.gene38740:CDS |
| TTCAAAGCACTTGTTAGCAT+TGG | 0.451055 | 3.3:+83943403 | None:intergenic |
| ACCTACCGCATCTGATCATA+AGG | 0.451382 | 3.3:-83943176 | MS.gene38740:CDS |
| TTAAAAGACAGTGCCACAAC+AGG | 0.477843 | 3.3:-83943511 | MS.gene38740:CDS |
| TTGCAACCAGATGTTAACAT+AGG | 0.495244 | 3.3:+83943571 | None:intergenic |
| TCACTCCTTCGCCACACAAC+AGG | 0.500035 | 3.3:-83943652 | MS.gene38740:CDS |
| AATATTAATAATGGTGCTCA+AGG | 0.504970 | 3.3:-83943052 | MS.gene38740:CDS |
| AGGTGTGTCCATGGGAGCTG+GGG | 0.517152 | 3.3:-83943203 | MS.gene38740:CDS |
| AGGTAACACCCCAGCTCCCA+TGG | 0.530725 | 3.3:+83943195 | None:intergenic |
| AAGCGCAACAAATTCTCTGC+AGG | 0.533172 | 3.3:-83943622 | MS.gene38740:CDS |
| TGCAACCAGATGTTAACATA+GGG | 0.541359 | 3.3:+83943572 | None:intergenic |
| GATCGCCTGTTGTGTGGCGA+AGG | 0.553228 | 3.3:+83943647 | None:intergenic |
| TGATATTGTTAGAATCGCGT+TGG | 0.555089 | 3.3:-83943365 | MS.gene38740:CDS |
| GATCATAAGGAAAGTTCTGA+TGG | 0.563793 | 3.3:-83943163 | MS.gene38740:CDS |
| TCAATTAGGAGGAAACAAAT+GGG | 0.565629 | 3.3:-83943259 | MS.gene38740:CDS |
| GCATTTGAGAGGTGTGTCCA+TGG | 0.565962 | 3.3:-83943212 | MS.gene38740:CDS |
| CAAGTTATGCAGCATTTGAG+AGG | 0.568598 | 3.3:-83943223 | MS.gene38740:CDS |
| CATGGACTAGAGTGCACAGA+TGG | 0.570529 | 3.3:+83943703 | None:intergenic |
| CGCAACAAATTCTCTGCAGG+AGG | 0.576933 | 3.3:-83943619 | MS.gene38740:CDS |
| CTCTAGTCCATGCTATACAA+TGG | 0.577403 | 3.3:-83943692 | MS.gene38740:CDS |
| GTTGTTGGAAGAGTTTGTTT+GGG | 0.579676 | 3.3:-83943314 | MS.gene38740:CDS |
| CATTTGAGAGGTGTGTCCAT+GGG | 0.583648 | 3.3:-83943211 | MS.gene38740:CDS |
| AAGTTGGGTATTCAATTAGG+AGG | 0.586438 | 3.3:-83943270 | MS.gene38740:CDS |
| ACCAAAGATTGTTCCTGTTG+TGG | 0.612454 | 3.3:+83943498 | None:intergenic |
| TCCTTATGATCAGATGCGGT+AGG | 0.615081 | 3.3:+83943175 | None:intergenic |
| ACTTTCCTTATGATCAGATG+CGG | 0.640661 | 3.3:+83943171 | None:intergenic |
| GACCAAATGACTTATATGAG+AGG | 0.654037 | 3.3:-83943139 | MS.gene38740:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CCATTATTAATATTATTATC+AGG | + | chr3.3:83943663-83943682 | None:intergenic | 15.0% |
| !! | CCTGATAATAATATTAATAA+TGG | - | chr3.3:83943660-83943679 | MS.gene38740:CDS | 15.0% |
| ! | AATATTAATAATGGTGCTCA+AGG | - | chr3.3:83943669-83943688 | MS.gene38740:CDS | 25.0% |
| ! | ACAAAAAATATGCTAAACTC+AGG | + | chr3.3:83943696-83943715 | None:intergenic | 25.0% |
| !! | ATTGTAATGGAAGAAAAGTT+GGG | - | chr3.3:83943436-83943455 | MS.gene38740:CDS | 25.0% |
| !! | TATTGTAATGGAAGAAAAGT+TGG | - | chr3.3:83943435-83943454 | MS.gene38740:CDS | 25.0% |
| AACGAATAAGAAGAAGTTGT+TGG | - | chr3.3:83943392-83943411 | MS.gene38740:CDS | 30.0% | |
| ACAATCTTTGGTTACAGAAA+AGG | - | chr3.3:83943234-83943253 | MS.gene38740:CDS | 30.0% | |
| GAAAAGTTGGGTATTCAATT+AGG | - | chr3.3:83943448-83943467 | MS.gene38740:CDS | 30.0% | |
| GGTAACTCAATGAGAAAAAT+CGG | + | chr3.3:83943300-83943319 | None:intergenic | 30.0% | |
| GGTAGATTTGAAAGAGTAAT+TGG | - | chr3.3:83943603-83943622 | MS.gene38740:CDS | 30.0% | |
| TACCTCTCATATAAGTCATT+TGG | + | chr3.3:83943587-83943606 | None:intergenic | 30.0% | |
| TCAATTAGGAGGAAACAAAT+GGG | - | chr3.3:83943462-83943481 | MS.gene38740:CDS | 30.0% | |
| TTCAATTAGGAGGAAACAAA+TGG | - | chr3.3:83943461-83943480 | MS.gene38740:CDS | 30.0% | |
| ! | TCTTTTAACATAGGCTTTCT+TGG | + | chr3.3:83943198-83943217 | None:intergenic | 30.0% |
| !! | TGAAAGAGTAATTGGTTCTA+AGG | - | chr3.3:83943611-83943630 | MS.gene38740:CDS | 30.0% |
| AAGTTGGGTATTCAATTAGG+AGG | - | chr3.3:83943451-83943470 | MS.gene38740:CDS | 35.0% | |
| ACTTTCCTTATGATCAGATG+CGG | + | chr3.3:83943553-83943572 | None:intergenic | 35.0% | |
| GACCAAATGACTTATATGAG+AGG | - | chr3.3:83943582-83943601 | MS.gene38740:CDS | 35.0% | |
| GTTAACATCTGGTTGCAAAA+TGG | - | chr3.3:83943155-83943174 | MS.gene38740:CDS | 35.0% | |
| GTTTGGGCTGTTTATTGTAA+TGG | - | chr3.3:83943423-83943442 | MS.gene38740:CDS | 35.0% | |
| TGATATTGTTAGAATCGCGT+TGG | - | chr3.3:83943356-83943375 | MS.gene38740:CDS | 35.0% | |
| TGCAAAATGGTAGCACTATT+AGG | - | chr3.3:83943168-83943187 | MS.gene38740:CDS | 35.0% | |
| TGCAACCAGATGTTAACATA+GGG | + | chr3.3:83943152-83943171 | None:intergenic | 35.0% | |
| TTGCAACCAGATGTTAACAT+AGG | + | chr3.3:83943153-83943172 | None:intergenic | 35.0% | |
| ! | AGTTGTTGGAAGAGTTTGTT+TGG | - | chr3.3:83943406-83943425 | MS.gene38740:CDS | 35.0% |
| ! | GATCATAAGGAAAGTTCTGA+TGG | - | chr3.3:83943558-83943577 | MS.gene38740:CDS | 35.0% |
| ! | GGATTTTCCATTGTATAGCA+TGG | + | chr3.3:83943039-83943058 | None:intergenic | 35.0% |
| ! | GTTGTTGGAAGAGTTTGTTT+GGG | - | chr3.3:83943407-83943426 | MS.gene38740:CDS | 35.0% |
| ! | TTCAAAGCACTTGTTAGCAT+TGG | + | chr3.3:83943321-83943340 | None:intergenic | 35.0% |
| ACCAAAGATTGTTCCTGTTG+TGG | + | chr3.3:83943226-83943245 | None:intergenic | 40.0% | |
| CAAGTTATGCAGCATTTGAG+AGG | - | chr3.3:83943498-83943517 | MS.gene38740:CDS | 40.0% | |
| CTCTAGTCCATGCTATACAA+TGG | - | chr3.3:83943029-83943048 | MS.gene38740:CDS | 40.0% | |
| CTCTTCCCTATGTTAACATC+TGG | - | chr3.3:83943144-83943163 | MS.gene38740:CDS | 40.0% | |
| TTAAAAGACAGTGCCACAAC+AGG | - | chr3.3:83943210-83943229 | MS.gene38740:CDS | 40.0% | |
| ! | GTGGCACTGTCTTTTAACAT+AGG | + | chr3.3:83943207-83943226 | None:intergenic | 40.0% |
| AAGCGCAACAAATTCTCTGC+AGG | - | chr3.3:83943099-83943118 | MS.gene38740:CDS | 45.0% | |
| ACCTACCGCATCTGATCATA+AGG | - | chr3.3:83943545-83943564 | MS.gene38740:CDS | 45.0% | |
| GCCACAACAGGAACAATCTT+TGG | - | chr3.3:83943222-83943241 | MS.gene38740:CDS | 45.0% | |
| TCCTTATGATCAGATGCGGT+AGG | + | chr3.3:83943549-83943568 | None:intergenic | 45.0% | |
| ! | CATTTGAGAGGTGTGTCCAT+GGG | - | chr3.3:83943510-83943529 | MS.gene38740:CDS | 45.0% |
| CGAAGGAGTGATGCTATTGC+TGG | + | chr3.3:83943060-83943079 | None:intergenic | 50.0% | |
| CGCAACAAATTCTCTGCAGG+AGG | - | chr3.3:83943102-83943121 | MS.gene38740:CDS | 50.0% | |
| ! | GCATTTGAGAGGTGTGTCCA+TGG | - | chr3.3:83943509-83943528 | MS.gene38740:CDS | 50.0% |
| ! | GCTTTTGATCGCCTGTTGTG+TGG | + | chr3.3:83943083-83943102 | None:intergenic | 50.0% |
| !! | CATGGACTAGAGTGCACAGA+TGG | + | chr3.3:83943021-83943040 | None:intergenic | 50.0% |
| TCACTCCTTCGCCACACAAC+AGG | - | chr3.3:83943069-83943088 | MS.gene38740:CDS | 55.0% | |
| AGGTAACACCCCAGCTCCCA+TGG | + | chr3.3:83943529-83943548 | None:intergenic | 60.0% | |
| AGGTGTGTCCATGGGAGCTG+GGG | - | chr3.3:83943518-83943537 | MS.gene38740:CDS | 60.0% | |
| GAGGTGTGTCCATGGGAGCT+GGG | - | chr3.3:83943517-83943536 | MS.gene38740:CDS | 60.0% | |
| GATCGCCTGTTGTGTGGCGA+AGG | + | chr3.3:83943077-83943096 | None:intergenic | 60.0% | |
| ! | AGAGGTGTGTCCATGGGAGC+TGG | - | chr3.3:83943516-83943535 | MS.gene38740:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 83943015 | 83943728 | 83943015 | ID=MS.gene38740 |
| chr3.3 | mRNA | 83943015 | 83943728 | 83943015 | ID=MS.gene38740.t1;Parent=MS.gene38740 |
| chr3.3 | exon | 83943015 | 83943728 | 83943015 | ID=MS.gene38740.t1.exon1;Parent=MS.gene38740.t1 |
| chr3.3 | CDS | 83943015 | 83943728 | 83943015 | ID=cds.MS.gene38740.t1;Parent=MS.gene38740.t1 |
| Gene Sequence |
| Protein sequence |
