AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene054618


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054618.t1 MTR_3g101300 99.578 237 1 0 1 237 1 237 3.73e-180 493
MS.gene054618.t1 MTR_8g040690 41.564 243 120 9 2 236 19 247 2.79e-53 172
MS.gene054618.t1 MTR_3g094830 45.098 204 96 7 41 235 62 258 5.34e-52 169
MS.gene054618.t1 MTR_8g040680 43.689 206 103 6 36 236 60 257 1.95e-51 168
MS.gene054618.t1 MTR_2g054380 49.102 167 76 3 72 235 109 269 2.48e-48 160
MS.gene054618.t1 MTR_4g112370 46.821 173 77 6 69 235 96 259 1.58e-44 150
MS.gene054618.t1 MTR_2g055990 45.000 180 87 6 60 235 87 258 4.22e-43 146
MS.gene054618.t1 MTR_1g053130 45.665 173 79 4 72 235 117 283 2.08e-39 137
MS.gene054618.t1 MTR_3g086740 42.458 179 86 7 65 236 75 243 6.22e-38 132
MS.gene054618.t1 MTR_7g064810 41.667 180 87 6 60 236 59 223 3.16e-35 125
MS.gene054618.t1 MTR_8g089865 40.000 170 89 7 73 236 59 221 3.10e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene054618.t1 AT5G42680 61.885 244 80 5 1 237 1 238 5.50e-101 293
MS.gene054618.t1 AT5G42680 61.885 244 80 5 1 237 1 238 5.50e-101 293
MS.gene054618.t1 AT4G39610 46.581 234 99 10 20 236 40 264 2.40e-58 186
MS.gene054618.t1 AT5G06990 43.333 240 114 8 8 236 33 261 4.56e-54 175
MS.gene054618.t1 AT2G21990 47.291 203 92 7 41 236 58 252 1.32e-53 173
MS.gene054618.t1 AT3G25640 50.000 172 72 7 73 235 100 266 8.87e-49 161
MS.gene054618.t1 AT3G25640 50.000 172 72 7 73 235 126 292 1.65e-48 161
MS.gene054618.t1 AT2G41660 44.509 173 83 4 71 235 129 296 2.22e-44 151
MS.gene054618.t1 AT5G23100 42.051 195 79 5 69 235 88 276 3.33e-40 139
MS.gene054618.t1 AT2G37880 43.617 188 88 8 55 236 70 245 7.82e-40 137
MS.gene054618.t1 AT1G21050 41.176 170 81 6 73 236 89 245 5.97e-36 127
MS.gene054618.t1 AT1G21050 41.176 170 81 6 73 236 125 281 1.06e-35 128
MS.gene054618.t1 AT5G65340 40.571 175 86 5 72 236 84 250 3.72e-32 118
MS.gene054618.t1 AT1G76610 38.235 170 84 6 70 236 75 226 1.22e-29 110
MS.gene054618.t1 AT2G22460 36.145 166 95 4 71 234 85 241 9.32e-26 101

Find 46 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAGATTTGAAAGAGTAAT+TGG 0.213989 3.4:-89196960 MS.gene054618:CDS
TGAAAGAGTAATTGGTTCTA+AGG 0.266032 3.4:-89196952 MS.gene054618:CDS
GAAAAGTTGGGTATTCAATT+AGG 0.280770 3.4:-89197115 MS.gene054618:CDS
AGAGGTGTGTCCATGGGAGC+TGG 0.305516 3.4:-89197047 MS.gene054618:CDS
CCATTATTAATATTATTATC+AGG 0.336954 3.4:+89196903 None:intergenic
GCCACAACAGGAACAATCTT+TGG 0.337368 3.4:-89197341 MS.gene054618:CDS
CCTGATAATAATATTAATAA+TGG 0.346292 3.4:-89196903 MS.gene054618:CDS
TATTGTAATGGAAGAAAAGT+TGG 0.347703 3.4:-89197128 MS.gene054618:CDS
AGTTGTTGGAAGAGTTTGTT+TGG 0.358226 3.4:-89197157 MS.gene054618:CDS
TGCAAAATGGTAGCACTATT+AGG 0.363693 3.4:-89197395 MS.gene054618:CDS
TTCAATTAGGAGGAAACAAA+TGG 0.393295 3.4:-89197102 MS.gene054618:CDS
TACCTCTCATATAAGTCATT+TGG 0.396969 3.4:+89196979 None:intergenic
GTTTGGGCTGTTTATTGTAA+TGG 0.399156 3.4:-89197140 MS.gene054618:CDS
GTTAACATCAGGTTGCAAAA+TGG 0.412225 3.4:-89197408 MS.gene054618:CDS
ACAATCTTTGGTTACAGAAA+AGG 0.421379 3.4:-89197329 MS.gene054618:CDS
CGAAGGAGTGATGCTATTGC+TGG 0.423322 3.4:+89197506 None:intergenic
ATTGTAATGGAAGAAAAGTT+GGG 0.435261 3.4:-89197127 MS.gene054618:CDS
AACGAATAAGAAGAAGTTGT+TGG 0.437700 3.4:-89197171 MS.gene054618:CDS
CTCTTCCCTATGTTAACATC+AGG 0.442390 3.4:-89197419 MS.gene054618:CDS
GAGGTGTGTCCATGGGAGCT+GGG 0.448845 3.4:-89197046 MS.gene054618:CDS
TTCAAAGCACTTGTTAGCAT+TGG 0.451055 3.4:+89197245 None:intergenic
ACCTACCGCATCTGATCATA+AGG 0.451382 3.4:-89197018 MS.gene054618:CDS
TTGCAACCTGATGTTAACAT+AGG 0.475328 3.4:+89197413 None:intergenic
TTAAAAGACAGTGCCACAAC+AGG 0.477843 3.4:-89197353 MS.gene054618:CDS
TCACTCCTTCGCCACACAAC+AGG 0.500035 3.4:-89197494 MS.gene054618:CDS
AATATTAATAATGGTGCTCA+AGG 0.504970 3.4:-89196894 MS.gene054618:CDS
AGGTGTGTCCATGGGAGCTG+GGG 0.517152 3.4:-89197045 MS.gene054618:CDS
AGGTAACACCCCAGCTCCCA+TGG 0.530725 3.4:+89197037 None:intergenic
AAGCGCAACAAATTCTCTGC+AGG 0.533172 3.4:-89197464 MS.gene054618:CDS
TGCAACCTGATGTTAACATA+GGG 0.538919 3.4:+89197414 None:intergenic
GATCGCCTGTTGTGTGGCGA+AGG 0.553228 3.4:+89197489 None:intergenic
TGATATTGTTAGAATCGCGT+TGG 0.555089 3.4:-89197207 MS.gene054618:CDS
GATCATAAGGAAAGTTCTGA+TGG 0.563793 3.4:-89197005 MS.gene054618:CDS
TCAATTAGGAGGAAACAAAT+GGG 0.565629 3.4:-89197101 MS.gene054618:CDS
GCATTTGAGAGGTGTGTCCA+TGG 0.565962 3.4:-89197054 MS.gene054618:CDS
CAAGTTATGCAGCATTTGAG+AGG 0.568598 3.4:-89197065 MS.gene054618:CDS
CATGGACTAGAGTGCACAGA+TGG 0.570529 3.4:+89197545 None:intergenic
CGCAACAAATTCTCTGCAGG+AGG 0.576933 3.4:-89197461 MS.gene054618:CDS
CTCTAGTCCATGCTATACAA+TGG 0.577403 3.4:-89197534 MS.gene054618:CDS
GTTGTTGGAAGAGTTTGTTT+GGG 0.579676 3.4:-89197156 MS.gene054618:CDS
CATTTGAGAGGTGTGTCCAT+GGG 0.583648 3.4:-89197053 MS.gene054618:CDS
AAGTTGGGTATTCAATTAGG+AGG 0.586438 3.4:-89197112 MS.gene054618:CDS
ACCAAAGATTGTTCCTGTTG+TGG 0.612454 3.4:+89197340 None:intergenic
TCCTTATGATCAGATGCGGT+AGG 0.615081 3.4:+89197017 None:intergenic
ACTTTCCTTATGATCAGATG+CGG 0.640661 3.4:+89197013 None:intergenic
GACCAAATGACTTATATGAG+AGG 0.654037 3.4:-89196981 MS.gene054618:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CCATTATTAATATTATTATC+AGG + chr3.4:89197505-89197524 None:intergenic 15.0%
!! CCTGATAATAATATTAATAA+TGG - chr3.4:89197502-89197521 MS.gene054618:CDS 15.0%
! AATATTAATAATGGTGCTCA+AGG - chr3.4:89197511-89197530 MS.gene054618:CDS 25.0%
! ACAAAAAAGATACTAAACTC+AGG + chr3.4:89197538-89197557 None:intergenic 25.0%
!! ATTGTAATGGAAGAAAAGTT+GGG - chr3.4:89197278-89197297 MS.gene054618:CDS 25.0%
!! TATTGTAATGGAAGAAAAGT+TGG - chr3.4:89197277-89197296 MS.gene054618:CDS 25.0%
AACGAATAAGAAGAAGTTGT+TGG - chr3.4:89197234-89197253 MS.gene054618:CDS 30.0%
ACAATCTTTGGTTACAGAAA+AGG - chr3.4:89197076-89197095 MS.gene054618:CDS 30.0%
GAAAAGTTGGGTATTCAATT+AGG - chr3.4:89197290-89197309 MS.gene054618:CDS 30.0%
GGTAACTCAATGAGAAAAAT+CGG + chr3.4:89197142-89197161 None:intergenic 30.0%
GGTAGATTTGAAAGAGTAAT+TGG - chr3.4:89197445-89197464 MS.gene054618:CDS 30.0%
TACCTCTCATATAAGTCATT+TGG + chr3.4:89197429-89197448 None:intergenic 30.0%
TCAATTAGGAGGAAACAAAT+GGG - chr3.4:89197304-89197323 MS.gene054618:CDS 30.0%
TTCAATTAGGAGGAAACAAA+TGG - chr3.4:89197303-89197322 MS.gene054618:CDS 30.0%
! TCTTTTAACATAGGCTTTCT+TGG + chr3.4:89197040-89197059 None:intergenic 30.0%
!! TGAAAGAGTAATTGGTTCTA+AGG - chr3.4:89197453-89197472 MS.gene054618:CDS 30.0%
AAGTTGGGTATTCAATTAGG+AGG - chr3.4:89197293-89197312 MS.gene054618:CDS 35.0%
ACTTTCCTTATGATCAGATG+CGG + chr3.4:89197395-89197414 None:intergenic 35.0%
GACCAAATGACTTATATGAG+AGG - chr3.4:89197424-89197443 MS.gene054618:CDS 35.0%
GTTTGGGCTGTTTATTGTAA+TGG - chr3.4:89197265-89197284 MS.gene054618:CDS 35.0%
TGATATTGTTAGAATCGCGT+TGG - chr3.4:89197198-89197217 MS.gene054618:CDS 35.0%
TGCAAAATGGTAGCACTATT+AGG - chr3.4:89197010-89197029 MS.gene054618:CDS 35.0%
TGCAACCTGATGTTAACATA+GGG + chr3.4:89196994-89197013 None:intergenic 35.0%
TTGCAACCTGATGTTAACAT+AGG + chr3.4:89196995-89197014 None:intergenic 35.0%
! AGTTGTTGGAAGAGTTTGTT+TGG - chr3.4:89197248-89197267 MS.gene054618:CDS 35.0%
! GATCATAAGGAAAGTTCTGA+TGG - chr3.4:89197400-89197419 MS.gene054618:CDS 35.0%
! GGATTTTCCATTGTATAGCA+TGG + chr3.4:89196881-89196900 None:intergenic 35.0%
! GTTAACATCAGGTTGCAAAA+TGG - chr3.4:89196997-89197016 MS.gene054618:CDS 35.0%
! GTTGTTGGAAGAGTTTGTTT+GGG - chr3.4:89197249-89197268 MS.gene054618:CDS 35.0%
! TTCAAAGCACTTGTTAGCAT+TGG + chr3.4:89197163-89197182 None:intergenic 35.0%
ACCAAAGATTGTTCCTGTTG+TGG + chr3.4:89197068-89197087 None:intergenic 40.0%
CAAGTTATGCAGCATTTGAG+AGG - chr3.4:89197340-89197359 MS.gene054618:CDS 40.0%
CTCTAGTCCATGCTATACAA+TGG - chr3.4:89196871-89196890 MS.gene054618:CDS 40.0%
CTCTTCCCTATGTTAACATC+AGG - chr3.4:89196986-89197005 MS.gene054618:CDS 40.0%
TTAAAAGACAGTGCCACAAC+AGG - chr3.4:89197052-89197071 MS.gene054618:CDS 40.0%
! GTGGCACTGTCTTTTAACAT+AGG + chr3.4:89197049-89197068 None:intergenic 40.0%
AAGCGCAACAAATTCTCTGC+AGG - chr3.4:89196941-89196960 MS.gene054618:CDS 45.0%
ACCTACCGCATCTGATCATA+AGG - chr3.4:89197387-89197406 MS.gene054618:CDS 45.0%
GCCACAACAGGAACAATCTT+TGG - chr3.4:89197064-89197083 MS.gene054618:CDS 45.0%
TCCTTATGATCAGATGCGGT+AGG + chr3.4:89197391-89197410 None:intergenic 45.0%
! CATTTGAGAGGTGTGTCCAT+GGG - chr3.4:89197352-89197371 MS.gene054618:CDS 45.0%
CGAAGGAGTGATGCTATTGC+TGG + chr3.4:89196902-89196921 None:intergenic 50.0%
CGCAACAAATTCTCTGCAGG+AGG - chr3.4:89196944-89196963 MS.gene054618:CDS 50.0%
! GCATTTGAGAGGTGTGTCCA+TGG - chr3.4:89197351-89197370 MS.gene054618:CDS 50.0%
! GCTTTTGATCGCCTGTTGTG+TGG + chr3.4:89196925-89196944 None:intergenic 50.0%
!! CATGGACTAGAGTGCACAGA+TGG + chr3.4:89196863-89196882 None:intergenic 50.0%
TCACTCCTTCGCCACACAAC+AGG - chr3.4:89196911-89196930 MS.gene054618:CDS 55.0%
AGGTAACACCCCAGCTCCCA+TGG + chr3.4:89197371-89197390 None:intergenic 60.0%
AGGTGTGTCCATGGGAGCTG+GGG - chr3.4:89197360-89197379 MS.gene054618:CDS 60.0%
GAGGTGTGTCCATGGGAGCT+GGG - chr3.4:89197359-89197378 MS.gene054618:CDS 60.0%
GATCGCCTGTTGTGTGGCGA+AGG + chr3.4:89196919-89196938 None:intergenic 60.0%
! AGAGGTGTGTCCATGGGAGC+TGG - chr3.4:89197358-89197377 MS.gene054618:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 89196857 89197570 89196857 ID=MS.gene054618
chr3.4 mRNA 89196857 89197570 89196857 ID=MS.gene054618.t1;Parent=MS.gene054618
chr3.4 exon 89196857 89197570 89196857 ID=MS.gene054618.t1.exon1;Parent=MS.gene054618.t1
chr3.4 CDS 89196857 89197570 89196857 ID=cds.MS.gene054618.t1;Parent=MS.gene054618.t1
Gene Sequence

>MS.gene054618

ATGCCATCTGTGCACTCTAGTCCATGCTATACAATGGAAAATCCAGCAATAGCATCACTCCTTCGCCACACAACAGGCGATCAAAAGCGCAACAAATTCTCTGCAGGAGGTCTCTTAAAGATGTTCAAACTCTTCCCTATGTTAACATCAGGTTGCAAAATGGTAGCACTATTAGGAAGACCAAGAAAGCCTATGTTAAAAGACAGTGCCACAACAGGAACAATCTTTGGTTACAGAAAAGGAAGAGTTAGCATAGCAATACAAGAAGATACGCGACAAATGCCGATTTTTCTCATTGAGTTACCAATGCTAACAAGTGCTTTGAACAAAGAAATGTCGTCTGATATTGTTAGAATCGCGTTGGAGAGTGAGACGAAAACGAATAAGAAGAAGTTGTTGGAAGAGTTTGTTTGGGCTGTTTATTGTAATGGAAGAAAAGTTGGGTATTCAATTAGGAGGAAACAAATGGGTGATGAAGAACTTCAAGTTATGCAGCATTTGAGAGGTGTGTCCATGGGAGCTGGGGTGTTACCTACCGCATCTGATCATAAGGAAAGTTCTGATGGTGACCAAATGACTTATATGAGAGGTAGATTTGAAAGAGTAATTGGTTCTAAGGATTCTGAAGCATTTTATATGATTAATCCTGATAATAATATTAATAATGGTGCTCAAGGACCTGAGTTTAGTATCTTTTTTGTTAGAGCTCATTAG

Protein sequence

>MS.gene054618.t1

MPSVHSSPCYTMENPAIASLLRHTTGDQKRNKFSAGGLLKMFKLFPMLTSGCKMVALLGRPRKPMLKDSATTGTIFGYRKGRVSIAIQEDTRQMPIFLIELPMLTSALNKEMSSDIVRIALESETKTNKKKLLEEFVWAVYCNGRKVGYSIRRKQMGDEELQVMQHLRGVSMGAGVLPTASDHKESSDGDQMTYMRGRFERVIGSKDSEAFYMINPDNNINNGAQGPEFSIFFVRAH